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Dr. Maria Doyle ([email protected]) and Dr. Stefano Mangiola ([email protected]) | ||
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# Workshop Description | ||
## Workshop Description | ||
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This workshop will present how to perform analysis of RNA sequencing data following the tidy data paradigm. The tidy data paradigm provides a standard way to organise data values within a dataset, where each variable is a column, each observation is a row, and data is manipulated using an easy-to-understand vocabulary. Most importantly, the data structure remains consistent across manipulation and analysis functions. | ||
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* Familiarity with tidyverse syntax | ||
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Recommended Background Reading | ||
[Introduction to R for Biologists](https://mblue9.github.io/r-intro-biologists/intro_r_biologists.html) | ||
[Introduction to R for Biologists](https://melbournebioinformatics.github.io/r-intro-biologists/intro_r_biologists.html) | ||
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## Workshop Participation | ||
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Students will be expected to participate in the workshop in a hands-on way, following along with the code provided and performing exercises. | ||
Students will be expected to participate in the workshop in a hands-on way, following along with the code provided and performing exercises. | ||
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## _R_ / _Bioconductor_ packages used | ||
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* tidyverse | ||
* tidybulk | ||
* tidyHeatmap | ||
* edgeR | ||
* devtools | ||
* ggrepel | ||
* airway | ||
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## Time outline | ||
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| Activity | Time | | ||
|----------------------------------------------|------| | ||
| Data exploration | 30m | | ||
| Data dimensionality reduction and clustering | 30m | | ||
| Differential gene expression | 30m | | ||
| Data visualisation | 30m | | ||
| Data preprocessing | 15m | | ||
| Data dimensionality reduction and clustering | 15m | | ||
| Differential gene expression | 10m | | ||
| Data visualisation | 20m | | ||
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# Workshop goals and objectives | ||
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* Recall the key concepts of RNA sequencing data analysis | ||
* Apply the concepts to publicly available data | ||
* Create plots that summarise the information content of the data and analysis results | ||
* Create plots that summarise the information content of the data and analysis results |