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Work w/ Alison
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Ivica Ceraj committed Mar 27, 2013
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8 changes: 8 additions & 0 deletions html_app/assignments/mit706s13/gen/instructions.gss.css
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4 changes: 2 additions & 2 deletions html_app/assignments/mit706s13/gen/instructions.soy.js

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9 changes: 9 additions & 0 deletions html_app/assignments/mit706s13/instructions.gss
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17 changes: 8 additions & 9 deletions html_app/assignments/mit706s13/instructions.soy
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{template .assignment_overview}
<div class='scb_s_mit706s13_assignment_overview' xmlns="http://www.w3.org/1999/html"
xmlns="http://www.w3.org/1999/html" xmlns="http://www.w3.org/1999/html">
This assignment is due at beginning of lecture (9:30 am) on Thursday, April 25th, 2013
This extra credit assignment is due at the beginning of lecture (9:30 am) on Thursday, April 25th, 2013.<br>
(Up to 5 points)
<h2>Goal</h2>
The goal of this assignment is to enhance learning of experimental design and analysis by providing you with the
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<div>1</div>
</div>
<div class='scb_objective_text'>
<p>

To further characterize the mutant strains, your advisor recommends that you first
analyze the mutant strains' DNA content by flow cytometry. While there are other more cost-prohibitive ways to
characterize cell cycle mutants, using expensive reagents, such as antibodies, she would like to keep costs low.
She hopes that the results of the DNA content analysis will provide the necessary data to further characterize
these cell cycle mutants and determine the arrest point at which the mutant strains are arresting.
</p>
<p>
Perform a flow cytometry experiment(s) to further characterize the Mutant 1 and Mutant 2 strains. Based on your
flow cytometry results and analysis, which cell cycle arrest point(s) are responsible for the phenotype you
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<div>2</div>
</div>
<div class='scb_objective_text'>
<p>

You present your flow cytometry experiment results and analyses to your faculty
advisor. She suggests that it will be beneficial to conduct other experiments to further characterize the mutant
strains. She urges you to perform western blotting to determine the protein levels and mobility of key cell
cycle proteins in the mutant strains when grown at the restrictive temperature and allows you to purchase many
antibodies to complete a thorough western blotting analysis. See the <b>Reference Information</b> (below) for more
information about these antibodies.
</p><p>
<p>
Perform a western blotting experiment(s) to further characterize the cell cycle defects in the Mutant 1 and
Mutant 2 strains. Based on your western blotting results, which cell cycle arrest point(s) are responsible for
the phenotype you observe for the Mutant 1 and Mutant 2 strains? For each mutant strain, describe how the
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experiments.
</li>
<li>
Chemical treatments. a) Cell cycle specific drugs that provide known, expected results under appropriate
Chemical treatments. <br>a) Cell cycle specific drugs that provide known, expected results under appropriate
conditions to which you may compare your experimental samples involving the mutant strains. The drugs are:
alpha factor = a mating pheromone, which results in a cell cycle arrest in G1; hydroxyurea = a drug that
depletes the cell of deoxyribonucleotides (dNTPs), which results in a cell cycle arrest in S-phase; and
nocodazole = a drug that inhibits polymerization of microtubules, which results in a cell cycle arrest in
mitosis. b) Protein Phosphatase 1 (PP1) = a phosphatase enzyme that removes phosphate groups from all
mitosis. <br>b) Other drugs. Protein Phosphatase 1 (PP1) = a phosphatase enzyme that removes phosphate groups from all
phosphorylated-serine, -threonine and -tyrosine amino acids.
</li>
<li>
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</tbody>

</table>
</li>
</ol>
<p>
For these experiments in StarCellBio you can assume that all of these antibodies recognize the phosphorylated
and
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cleavage sites:<br>
<img src='/static/assignments/mit706s13/assets/cleavage_sites.png'>
</p>
</li>
</ol>


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