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# Snakemake workflow: RNA-Seq-Pop | ||
# RNA-Seq-Pop | ||
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[![Snakemake](https://img.shields.io/badge/snakemake-≥5.11.0-brightgreen.svg)](https://snakemake.bitbucket.io) | ||
[![Build Status](https://travis-ci.org/sanjaynagi/rna-seq-ir.svg?branch=master)](https://travis-ci.org/snakemake-workflows/rna-seq-ir) | ||
[![Build Status](https://travis-ci.org/sanjaynagi/rna-seq-pop.svg?branch=master)](https://travis-ci.org/snakemake-workflows/rna-seq-pop) | ||
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This workflow performs various analyses of illumina paired-end RNA-Sequencing data: | ||
This snakemake workflow performs various analyses of illumina paired-end RNA-Sequencing data: | ||
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* Quality control of fastq reads with [FASTQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) | ||
* QC metrics integrated into one final QC report with [multiQC](https://multiqc.info/) | ||
* Differential expression analysis with [Kallisto](https://pachterlab.github.io/kallisto/) at the gene level ([DESeq2](https://bioconductor.org/packages/release/bioc/html/DESeq2.html)) and transcript level ([Sleuth](https://github.com/pachterlab/sleuth)) | ||
* Variant calling with [freebayes](https://github.com/freebayes/freebayes), and an Fst and [Population branch statistic (PBS)](https://science.sciencemag.org/content/329/5987/75) analysis, both in windows and at the gene-level ([Scikit-allel](https://scikit-allel.readthedocs.io/en/stable/)). | ||
* Various summary statistics are calculated (Wattersons Theta, Sequence Diversity etc) | ||
* Various summary statistics are calculated (Wattersons Theta, Sequence Diversity, Dxy etc) | ||
* Differential SNP testing with the R package [kissDE](https://bioconductor.org/packages/release/bioc/html/kissDE.html), which accounts for allele-specific expression. | ||
* Gene Set Enrichment analyses and Venn diagrams. | ||
* Allele counts at pre-specified loci of choice. | ||
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Whenever you want to synchronize your workflow copy with new developments from upstream, do the following. | ||
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1. Once, register the upstream repository in your local copy: `git remote add -f upstream [email protected]:snakemake-workflows/rna-seq-ir.git` or `git remote add -f upstream https://github.com/snakemake-workflows/rna-seq-ir.git` if you do not have setup ssh keys. | ||
1. Once, register the upstream repository in your local copy: `git remote add -f upstream [email protected]:snakemake-workflows/rna-seq-pop.git` or `git remote add -f upstream https://github.com/snakemake-workflows/rna-seq-pop.git` if you do not have setup ssh keys. | ||
2. Update the upstream version: `git fetch upstream`. | ||
3. Create a diff with the current version: `git diff HEAD upstream/master workflow > upstream-changes.diff`. | ||
4. Investigate the changes: `vim upstream-changes.diff`. | ||
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