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Update README.md
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sanjaynagi authored Nov 30, 2022
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Expand Up @@ -15,6 +15,9 @@ Primer design in *Anopheles gambiae s.l* taking into account SNP variation in pr

#### Release notes

- 0.5.12 - for cDNA primers, designing over exon-exon junctions is now optional due to `cDNA_exon_junction` argument. Docstring added to `designPrimers()`.
- 0.5.11 - feedback on troubleshooting now provided in `designPrimers()` function if primer design fails
- 0.5.10 - region string provided instead of contig
- 0.5.9 - 'cDNA primers' replaces 'qPCR primers' throughout.
- 0.5.8 - Two notebooks, short and long, including all in one function. can now check the primers for specificity with gget blat implementation. Most versions >0.4.0 were for a workshop and github actions CI.
- 0.4.0 - plot_primer_ag3_frequencies() now uses plotly to make an interactive plot, where one can hover over primer bases, which returns the exact frequency for each base.
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