Skip to content

Commit

Permalink
Merge pull request #169 from nf-core/affy_docs
Browse files Browse the repository at this point in the history
Affy docs
  • Loading branch information
pinin4fjords authored Oct 16, 2023
2 parents 8f78242 + 689a5a8 commit 94a2a71
Show file tree
Hide file tree
Showing 3 changed files with 20 additions and 4 deletions.
3 changes: 2 additions & 1 deletion CHANGELOG.md
Original file line number Diff line number Diff line change
Expand Up @@ -15,7 +15,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
- [[#137](https://github.com/nf-core/differentialabundance/pull/137)] - Add `--sizefactors_from_controls` and `--gene_id_col` for DESeq2 module to modules.config ([@WackerO](https://github.com/WackerO), review by [@pinin4fjords](https://github.com/pinin4fjords))
- [[#145](https://github.com/nf-core/differentialabundance/pull/145)] - Template update for nf-core/tools v2.9 ([@nf-core-bot](https://github.com/nf-core-bot), review by [@pinin4fjords](https://github.com/pinin4fjords), [@WackerO](https://github.com/WackerO))
- [[#147](https://github.com/nf-core/differentialabundance/pull/147)] - Add Maxquant analysis module ([@WackerO](https://github.com/WackerO), review by [@pinin4fjords](https://github.com/pinin4fjords))
- [[#166](https://github.com/nf-core/differentialabundance/issues/166)] - Output a parameter-resolved R Markdown document, as well as rendered HTML ([@pinin4fjords](https://github.com/pinin4fjords), review by )
- [[#166](https://github.com/nf-core/differentialabundance/issues/166)] - Output a parameter-resolved R Markdown document, as well as rendered HTML ([@pinin4fjords](https://github.com/pinin4fjords), review by [@WackerO](https://github.com/WackerO))
- [[#119](https://github.com/nf-core/differentialabundance/issues/119)] - Document sample sheet for Affy arrays ([@pinin4fjords](https://github.com/pinin4fjords), review by)

### `Fixed`

Expand Down
3 changes: 0 additions & 3 deletions assets/samplesheet.csv

This file was deleted.

18 changes: 18 additions & 0 deletions docs/usage.md
Original file line number Diff line number Diff line change
Expand Up @@ -39,6 +39,24 @@ TREATED_REP3,AEG588A2_S1_L004_R1_001.fastq.gz,AEG588A2_S1_L004_R2_001.fastq.gz,t

The file can be tab or comma separated.

### Affymetrix arrays

Abundances for Affy arrays are provided in CEL files within an archive. When creating sample sheets for Affy arrays, it's crucial to include a column that specifies which file corresponds to each sample. This file column is essential for linking each sample to its corresponding data file, as shown in the example below:

```
"file","id","name","patient","phenotype"
"GSM1229341_Gudjohnsson_001_6690_PP.CEL.gz","GSM1229341","p6690_PP","6690","lesional"
"GSM1229342_Gudjohnsson_002_6690_PN.CEL.gz","GSM1229342","p6690_PN","6690","uninvolved"
"GSM1229343_Gudjohnsson_003_7450_PN.CEL.gz","GSM1229343","p7450_PN","7450","uninvolved"
"GSM1229344_Gudjohnsson_004_7450_PP.CEL.gz","GSM1229344","p7450_PP","7450","lesional"
"GSM1229345_Gudjohnsson_005_7912_PP.CEL.gz","GSM1229345","p7912_PP","7912","lesional"
"GSM1229346_Gudjohnsson_006_7912_PN.CEL.gz","GSM1229346","p7912_PN","7912","uninvolved"
"GSM1229347_Gudjohnsson_007_8470_PP.CEL.gz","GSM1229347","p8470_PP","6690","lesional"
"GSM1229348_Gudjohnsson_008_8470_PN.CEL.gz","GSM1229348","p8470_PN","6690","uninvolved"
```

The "file" column in this example is used to specify the data file associated with each sample, which is essential for data analysis and interpretation.

## Abundance values

### RNA-seq and similar
Expand Down

0 comments on commit 94a2a71

Please sign in to comment.