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Merge branch '325-mixed-model-in-limma' of github.com:nf-core/differe…
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KamilMaliszArdigen committed Dec 3, 2024
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1 change: 0 additions & 1 deletion CHANGELOG.md
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Expand Up @@ -14,7 +14,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
- [[#354](https://github.com/nf-core/differentialabundance/pull/354)] - Warning message within the R Markdown report to control when genes don't have annotation data ([@alanmmobbs93](https://github.com/alanmmobbs93)). Review by [@WackerO](https://github.com/WackerO) and [@pinin4fjords](https://github.com/pinin4fjords).
- [[#325](https://github.com/nf-core/differentialabundance/pull/325)] - Integration of limma voom for rnaseq data with mixed models ([@KamilMaliszArdigen](https://github.com/KamilMaliszArdigen), review by [@pinin4fjords](https://github.com/pinin4fjords))


### Fixed

- [[#358](https://github.com/nf-core/differentialabundance/pull/358)] - Fixed nf-tests not running due to `--changed-since HEAD^`([@atrigila](https://github.com/atrigila), review by [@pinin4fjords](https://github.com/pinin4fjords))
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2 changes: 1 addition & 1 deletion docs/usage.md
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Expand Up @@ -274,6 +274,7 @@ With this configuration in place deployment should happen automatically every ti
There is also a [Shiny server application](https://posit.co/download/shiny-server/), which you can install on your own infrastruture and use to host applications yourself.

## Limma mixed models

> To use advanced mixed model in your analysis, you need to prepare your submission sheet by combining experimental factors into a single composite variable. See the necessary steps:
### Step 1: Create a Composite Factor Column
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After creating the composite variable (e.g., `Condition.Tissue`), use this column in your input contrast file to define the specific contrasts you want to test. Make sure all levels of the composite factor are correctly labeled and match the values in your contrast file.


## Gene set enrichment analysis

Currently, two tools can be used to do gene set enrichment analysis.
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