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Merge branch 'release/v2.2' of https://github.com/fidelram/deepTools
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dpryan79 committed Mar 1, 2016
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Expand Up @@ -76,7 +76,10 @@ Regular bigWig track
Separate tracks for each strand
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Sometimes it makes sense to generate two independent :ref:`bigWig` files for all reads on the forward and reverse strand, respectively.
Sometimes it makes sense to generate two independent :ref:`bigWig` files for all reads on the forward and reverse strand, respectively. As of deepTools version 2.2, one can simply use the ``--filterRNAstrand`` option, such as ``--filterRNAstrand forward`` or ``--filterRNAstrand reverse``. This handles paired-end and single-end datasets. For older versions of deepTools, please see the instructions below.

Versions before 2.2
*******************

To follow the examples, you need to know that ``-f`` will tell ``samtools view`` to **include** reads with the indicated flag, while ``-F`` will lead to the **exclusion** of reads with the respective flag.

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