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@@ -38,9 +38,9 @@ | |
\KOMAoptions{parskip=half}} | ||
\makeatother | ||
\usepackage{xcolor} | ||
\usepackage[top=30mm,left=30mm,heightrounded]{geometry} | ||
\usepackage[top=10mm,bottom=10mm,left=30mm,heightrounded]{geometry} | ||
\setlength{\emergencystretch}{3em} % prevent overfull lines | ||
\setcounter{secnumdepth}{-\maxdimen} % remove section numbering | ||
\setcounter{secnumdepth}{5} | ||
% Make \paragraph and \subparagraph free-standing | ||
\ifx\paragraph\undefined\else | ||
\let\oldparagraph\paragraph | ||
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@@ -114,7 +114,26 @@ | |
\usepackage[normalem]{ulem} | ||
\usepackage{makecell} | ||
\usepackage{xcolor} | ||
\usepackage{lineno} | ||
\usepackage{fancyhdr, graphicx} | ||
\usepackage[hidelinks]{hyperref} | ||
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\fancyhead{\vspace{70pt}} | ||
\renewcommand{\headrulewidth}{1.8pt} | ||
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\makeatletter | ||
\def\@maketitle{% | ||
\newpage | ||
\hfill\href{https://doi.org/10.24072/pci.archaeo.100389}{\includegraphics[width=3.5cm]{badge_PCI_Archaeology.png}}\hfill | ||
\null | ||
\begin{center} | ||
{\LARGE | ||
\textbf\newline{\@title} | ||
} | ||
%\newline | ||
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\end{center} | ||
\par} | ||
\makeatother | ||
\makeatletter | ||
\makeatother | ||
\makeatletter | ||
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@@ -196,15 +215,13 @@ | |
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\begin{document} | ||
%% modified from: https://github.com/quarto-journals/plos/blob/main/_extensions/plos/partials/before-body.tex | ||
\begin{flushleft} | ||
{\LARGE | ||
\textbf\newline{Multiproxy analysis exploring patterns of diet and | ||
disease in dental calculus and skeletal remains from a 19th century | ||
Dutch population} | ||
} | ||
\newline | ||
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\vspace{12pt} | ||
\maketitle | ||
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\begin{center} | ||
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\thispagestyle{fancy} | ||
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% Insert author names, affiliations and corresponding author email | ||
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\normalsize{Bjørn Peare | ||
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@@ -214,20 +231,23 @@ | |
Casna}\textsuperscript{1}, \normalsize{Menno | ||
Hoogland}\textsuperscript{1}, \normalsize{Historisch Genootschap | ||
Beemster}\textsuperscript{3}, \normalsize{Amanda G. | ||
Henry}\textsuperscript{1} | ||
\\ | ||
\bigskip | ||
\small{1} \small{Department of Archaeological Sciences, Leiden | ||
University}\\ \small{2} \small{Department of Forensic Medicine, Aarhus | ||
University}\\ \small{3} \small{Historisch Genootschap Beemster} | ||
\newline | ||
Henry}\textsuperscript{1}\vspace{12pt} | ||
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% Use the asterisk to denote corresponding authorship and provide email address in note below. | ||
* [email protected] | ||
\rule{\textwidth}{1.8pt}\vspace{8pt} | ||
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\end{flushleft} | ||
\end{center} | ||
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\linenumbers | ||
\textsuperscript{\footnotesize{1}}\textit{\small{Department of | ||
Archaeological Sciences, Leiden | ||
University}}\\ \textsuperscript{\footnotesize{2}}\textit{\small{Department | ||
of Forensic Medicine, Aarhus | ||
University}}\\ \textsuperscript{\footnotesize{3}}\textit{\small{Historisch | ||
Genootschap Beemster}} | ||
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% Use the asterisk to denote corresponding authorship and provide email address in note below. | ||
* \small{[email protected]} | ||
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\vspace{12pt} | ||
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\begin{abstract} | ||
Dental calculus is an excellent source of information on the dietary | ||
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@@ -270,12 +290,15 @@ | |
children and women. | ||
\end{abstract} | ||
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Keywords: dental calculus; LC-MS/MS; alkaloids; dental pathology; | ||
sinusitis; caffeine; tobacco | ||
\ifdefined\Shaded\renewenvironment{Shaded}{\begin{tcolorbox}[borderline west={3pt}{0pt}{shadecolor}, interior hidden, enhanced, boxrule=0pt, sharp corners, breakable, frame hidden]}{\end{tcolorbox}}\fi | ||
\small \textbf{Keywords:} dental calculus; LC-MS/MS; alkaloids; dental | ||
pathology; sinusitis; caffeine; tobacco \\ | ||
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\vfill | ||
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\newgeometry{top=30mm,left=30mm}\ifdefined\Shaded\renewenvironment{Shaded}{\begin{tcolorbox}[breakable, interior hidden, borderline west={3pt}{0pt}{shadecolor}, enhanced, boxrule=0pt, sharp corners, frame hidden]}{\end{tcolorbox}}\fi | ||
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\hypertarget{introduction}{% | ||
\subsection{Introduction}\label{introduction}} | ||
\section{Introduction}\label{introduction}} | ||
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Dental calculus has proven to be an excellent source of a wide variety | ||
of information about our past. The increased accessibility and | ||
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@@ -346,7 +369,7 @@ \subsection{Introduction}\label{introduction}} | |
identification of pipe notches in the dentition. | ||
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\hypertarget{materials}{% | ||
\subsection{Materials}\label{materials}} | ||
\section{Materials}\label{materials}} | ||
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The sample consists of 41 individuals from Middenbeemster, a 19th | ||
century rural Dutch site. The village of Middenbeemster and the | ||
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@@ -390,10 +413,10 @@ \subsection{Materials}\label{materials}} | |
\end{figure} | ||
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\hypertarget{methods}{% | ||
\subsection{Methods}\label{methods}} | ||
\section{Methods}\label{methods}} | ||
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\hypertarget{skeletal-analysis}{% | ||
\subsubsection{Skeletal analysis}\label{skeletal-analysis}} | ||
\subsection{Skeletal analysis}\label{skeletal-analysis}} | ||
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Demographic and pathological analyses were conducted in the Laboratory | ||
for Human Osteoarchaeology at Leiden University. Sex was estimated using | ||
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@@ -409,7 +432,7 @@ \subsubsection{Skeletal analysis}\label{skeletal-analysis}} | |
bones and the extent of taphonomic degradation. | ||
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\hypertarget{paleopathology}{% | ||
\paragraph{Paleopathology}\label{paleopathology}} | ||
\subsubsection{Paleopathology}\label{paleopathology}} | ||
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Pathological conditions and lesions that occur frequently in the | ||
population were included in the analysis. Data were dichotomised to | ||
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@@ -435,7 +458,7 @@ \subsubsection{Skeletal analysis}\label{skeletal-analysis}} | |
associated pitting. | ||
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\hypertarget{dental-pathology}{% | ||
\paragraph{Dental pathology}\label{dental-pathology}} | ||
\subsubsection{Dental pathology}\label{dental-pathology}} | ||
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Caries ratios were calculated by dividing the number of lesions by the | ||
number of teeth scored, resulting in a single caries ratio per | ||
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@@ -457,7 +480,7 @@ \subsubsection{Skeletal analysis}\label{skeletal-analysis}} | |
of each tooth to alveolar bone (Maat \& Mastwijk, 2005). | ||
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\hypertarget{calculus-sampling}{% | ||
\subsubsection{Calculus sampling}\label{calculus-sampling}} | ||
\subsection{Calculus sampling}\label{calculus-sampling}} | ||
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Where possible, we used material that had already been sampled for a | ||
previous study to prevent unnecessary repeated sampling of individuals. | ||
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@@ -479,7 +502,7 @@ \subsubsection{Calculus sampling}\label{calculus-sampling}} | |
chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. | ||
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\hypertarget{uhplc-msms}{% | ||
\subsubsection{UHPLC-MS/MS}\label{uhplc-msms}} | ||
\subsection{UHPLC-MS/MS}\label{uhplc-msms}} | ||
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The list of targeted compounds included both naturally occurring | ||
compounds known to have been used in the past, as well as synthetic | ||
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@@ -504,7 +527,7 @@ \subsubsection{UHPLC-MS/MS}\label{uhplc-msms}} | |
validation, see the original study by Sørensen and colleagues (2021). | ||
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\hypertarget{statistical-analysis}{% | ||
\subsubsection{Statistical analysis}\label{statistical-analysis}} | ||
\subsection{Statistical analysis}\label{statistical-analysis}} | ||
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All compounds and pathological conditions/lesions were converted to a | ||
presence/absence score. Pearson product-moment correlation was applied | ||
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@@ -524,7 +547,7 @@ \subsubsection{Statistical analysis}\label{statistical-analysis}} | |
were calculated with the \textbf{psych} package (Revelle, 2022). | ||
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\hypertarget{results}{% | ||
\subsection{Results}\label{results}} | ||
\section{Results}\label{results}} | ||
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Multiple compounds were detected in the dental calculus samples. | ||
Compounds detected at a lower concentration than the lower limit of | ||
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@@ -636,7 +659,7 @@ \subsection{Results}\label{results}} | |
nicotine and cotinine, and had no signs of a pipe notch. | ||
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\hypertarget{correlations-between-detected-alkaloids-and-diseases}{% | ||
\subsubsection{Correlations between detected alkaloids and | ||
\subsection{Correlations between detected alkaloids and | ||
diseases}\label{correlations-between-detected-alkaloids-and-diseases}} | ||
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For further statistical analyses, only the UHPLC-MS/MS results from | ||
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@@ -761,7 +784,7 @@ \subsubsection{Correlations between detected alkaloids and | |
\end{figure} | ||
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\hypertarget{discussion}{% | ||
\subsection{Discussion}\label{discussion}} | ||
\section{Discussion}\label{discussion}} | ||
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In this study we were able to extract and identify multiple alkaloids | ||
and salicylic acid from the dental calculus of individuals from | ||
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@@ -1042,7 +1065,7 @@ \subsection{Discussion}\label{discussion}} | |
pathways of dietary (and other) compounds. | ||
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\hypertarget{conclusions}{% | ||
\subsection{Conclusions}\label{conclusions}} | ||
\section{Conclusions}\label{conclusions}} | ||
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This preliminary study outlines the benefits of using calculus to target | ||
a variety of compounds that could have been consumed as medicine or | ||
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@@ -1064,8 +1087,8 @@ \subsection{Conclusions}\label{conclusions}} | |
whether dietary, medicinal, recreational, or otherwise. | ||
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\hypertarget{acknowledgements}{% | ||
\subsection*{Acknowledgements}\label{acknowledgements}} | ||
\addcontentsline{toc}{subsection}{Acknowledgements} | ||
\section*{Acknowledgements}\label{acknowledgements}} | ||
\addcontentsline{toc}{section}{Acknowledgements} | ||
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We wish to thank Kirsten Ziesemer for helping track down the calculus | ||
samples from her studies. Additionally, we owe special thanks to Vincent | ||
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@@ -1075,14 +1098,25 @@ \subsection*{Acknowledgements}\label{acknowledgements}} | |
preprint and providing helpful comments, and Mario Zimmerman and two | ||
other anonymous reviewers for their time and invaluable insights. | ||
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\hypertarget{funding}{% | ||
\section*{Funding}\label{funding}} | ||
\addcontentsline{toc}{section}{Funding} | ||
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This research has received funding from the European Research Council | ||
under the European Union's Horizon 2020 research and innovation program, | ||
grant agreement number STG--677576 (``HARVEST''). | ||
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\hypertarget{conflict-of-interest-disclosure}{% | ||
\section*{Conflict of interest | ||
disclosure}\label{conflict-of-interest-disclosure}} | ||
\addcontentsline{toc}{section}{Conflict of interest disclosure} | ||
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The authors have no conflicts to declare. | ||
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\hypertarget{data-availability-statement}{% | ||
\subsection*{Data Availability | ||
\section*{Data Availability | ||
Statement}\label{data-availability-statement}} | ||
\addcontentsline{toc}{subsection}{Data Availability Statement} | ||
\addcontentsline{toc}{section}{Data Availability Statement} | ||
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All raw data is available on Zenodo | ||
(\url{https://doi.org/10.5281/zenodo.8061483}). Analysis scripts, and | ||
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@@ -1091,16 +1125,9 @@ \subsection*{Data Availability | |
(\url{https://doi.org/10.5281/zenodo.7649825}) using the structure | ||
recommended by the \textbf{rrtools} R package (Marwick, 2019). | ||
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\hypertarget{conflict-of-interest-disclosure}{% | ||
\subsection*{Conflict of interest | ||
disclosure}\label{conflict-of-interest-disclosure}} | ||
\addcontentsline{toc}{subsection}{Conflict of interest disclosure} | ||
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The authors have no conflicts to declare. | ||
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\hypertarget{references}{% | ||
\subsection*{References}\label{references}} | ||
\addcontentsline{toc}{subsection}{References} | ||
\section*{References}\label{references}} | ||
\addcontentsline{toc}{section}{References} | ||
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\hypertarget{refs}{} | ||
\begin{CSLReferences}{1}{0} | ||
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\small{$it.number$} $if(it.name)$\small{$it.name$}$endif$ | ||
\textsuperscript{\footnotesize{$it.number$}}$if(it.name)$\textit{\small{$it.name$}}$endif$ |
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@@ -0,0 +1,20 @@ | ||
\usepackage{fancyhdr, graphicx} | ||
\usepackage[hidelinks]{hyperref} | ||
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\fancyhead{\vspace{70pt}} | ||
\renewcommand{\headrulewidth}{1.8pt} | ||
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\makeatletter | ||
\def\@maketitle{% | ||
\newpage | ||
\hfill\href{https://doi.org/10.24072/pci.archaeo.100389}{\includegraphics[width=3.5cm]{badge_PCI_Archaeology.png}}\hfill | ||
\null | ||
\begin{center} | ||
{\LARGE | ||
\textbf\newline{\@title} | ||
} | ||
%\newline | ||
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\end{center} | ||
\par} | ||
\makeatother |
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