RC HC3+HC4: Site specific recombination and horizontal gene transfer

Sofie Thijs ([email protected])

date: 8/10/2024

     

Here you will find the questions and resources to go through the various course topics related to mechanisms that induce genetic variation, repair, and to get familiar with their biotechnological applications.

Schedule

Time	Activity
11h00-11h15	Explanation how to go through the exercises
11h15-12h00	Examples of site-specific recombination and GATE-WAY cloning
12h00-12h50	Searching for transposable elements in eukaryotes
	10/10/2024
11h30-12h00	Searching for transposable elements in prokaryotes
12h00-12h45	Modifications to CRISPR-cas9
12h45-13h00	Wrap up and feedback

Learning outcomes

By the end of the RC, you should be able to:

• Explain what GATEWAY Cloning Technology is and on which natural mechanism it is based. You know that there are different GATEWAY expression vector systems, and you can give an example of how GATEWAY cloning is used.

• You can understand and discuss transposable elements in eukaryotes, and how it has contributed to genetic diversity

• You know how to use web-based databases for searching transposons, IS, phages, CRISPR in bacteria, fungi, plant and animal cells

• You can schematise the process of CRISPRcas9, give an example of an application, and you can discuss prime editing.

Course instructor

[email protected]