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MoonRatII, A Tote-Size Portable Incubator for Rapid Field Work

Aliases used by other developers include Moonrat, Rice MiniCubator, and Rice Petri FI. Visit the Legacy folder for the work of volunteers prior to December 2022 and the hand built prototype.

MoonratII development effort began January 2023.


Status, January 2025

Project presentation on YouTube (Click image to watch)

Watch the video

Updated Experiment.com succesful project funding round

In January 2023, 13 generous patrons funded the production of five complete MoonRatII prototypes. we updated them with the project results.

2024 field testing resulted in future unit changes:

  • Heater board redesign with a Dallas One wire digital temperature thermometer and a 4.7 ohm pull up resistor.
  • Design a universal heater board cage to secure the heater board beneath the container lid in any external enclosure.
  • Adjust top button spacing in KiCad.
  • Add bottom mounting holes to the enclosure.

Status, November 2024

A scientific research paper is tentatively scheduled for March 2025 in the HardwareX journal.

Status, September 2024

Prototypes of the MoonratII were field tested in two locations - the Galapagos Islands by researcher Auja Bywater and in Tanzania by Dr. Robert Read. The heater features the TMP36 low voltage temperature sensor. A HardwareX scientific research paper is slated for October 2024 publication.

Status, April 2024

The MoonRatII team has produced five Printed Circuit Assemblies (Control and Heater) of the Rev 1 PWB for use by the USA team and five Printed Circuit Assemblies (Control and Heater) of the Rev 2 PWB for use by the Mexico team. Enclosure design for the Controller Assembly of Rev 3 PWB is underway TBA.

Status January 2024

Dr. Robert Read and Melanie Laporte presented the MoonRatII project. Watch the presentation.

Current Design Summary

In one schematic and PCB, we have developed a "Control Assembly" and a "Heater Assembly".

  • The Control Assembly will be in an enclosure detachable from the incubation chamber.
  • The Heater Assembly will mounted internal to the incubation assembly under the unit top. Current to a heating pad or pads has changed from the previously used TMP36 low voltage temperature sensor to a digital temperature sensor.
  • A rechargable battery with its recharging station will complete a MoonRatII incubator system.

Image Gallery - Current FreeCAD Assembly Designs

A B C

2023 MoonRatII Design

Fundamental Modularity: The 6-wire appliance

The connections from the controller to the heater needs only 6 wires:

  1. GND (ground, nominally voltage 0).
  2. +12V PWR (the heating elements requires approximately 12V power).
  3. HEAT ON (Vin).
  4. +5V (for the thermometer).
  5. SIG - data from the digital "one-wire" format.
  6. UNK - a final wire is included for future functionality that is unknown right now. It is connected as a redundant ground in Rev 1 and Rev 2.

These (esp. the power) should probably be rated for 2 amps, but in practice 1 amp is probably good enough.

Initial Design Approach Block Diagram

From the 2020 design.
https://user-images.githubusercontent.com/5296671/86680945-b470ad80-bfc4-11ea-9ef6-19c4f70aceba.png
Basic Structure of Portable Incubator (1)

Motivation

By building a small, portable, intelligent incubator that can maintain constant elevated temperature, a variety of biological experiments and assays can be performed "in the field" without having access to a electric grid power.

Origin

This is an offshoot of the Rapid E. coli project. It is an attempt to build a better, smaller, more intelligent portable incubator that the Armadillo, described elsewhere and buildable from an instructable.

References (Incomplete)

Acknowledgements

Thanks to Dr. Sabia Abidi of Rice University for input and references.

MoonratII Team

Robert L. Read - Founder of Public Invention

F. Lee Erickson

Melanie Laporte

Silvia Castillas

Harshit Kumar

Enrique Ruiz

Horacio Garcia


Updated by Melanie Laporte, 20240512