TUT-tailing

Classification of templated and non-templated isomiRs

Example 1: Templated isomiR (miR-148b-3p)

     TCAGTGCATCACAGAACTTTGTc      read obtained
     |||||||||||||||||||||||
GAAAGTCAGTGCATCACAGAACTTTGTCTCGA  genomic reference

Example 2: Untemplated isomiR (miR-148b-3p)

     TCAGTGCATCACAGAACTTTGTt      read obtained
     ||||||||||||||||||||||0
GAAAGTCAGTGCATCACAGAACTTTGTCTCGA  genomic reference 

Example 3: Templated isomiR with multiple paralogs (miR-1-3p)

    TGGAATGTAAAGAAGTATGTATt      read obtained
    |||||||||||||||||||||||
GCTATGGAATGTAAAGAAGTATGTATCTCA  genomic reference paralog 1
GCTATGGAATGTAAAGAAGTATGTATTTTT  genomic reference paralog 2

In order to avoid confounding effects, if a read could be templated at least for one of the paralogs generating that miRNA, it was considered as a templated read.