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# nailpolish call | ||
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Consensus call duplicated reads | ||
The reads must first have been [indexed](./generate-index.md). | ||
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## Usage | ||
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```shell | ||
$ nailpolish call --help | ||
Generate a consensus-called 'cleaned up' file | ||
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Usage: nailpolish call [OPTIONS] --index <INDEX> --input <INPUT> | ||
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Options: | ||
--index <INDEX> the index file | ||
--input <INPUT> the input .fastq | ||
--output <OUTPUT> the output .fasta; note that quality values are not preserved | ||
-t, --threads <THREADS> the number of threads to use [default: 4] | ||
-d, --duplicates-only only show the duplicated reads, not the single ones | ||
-r, --report-original-reads for each duplicate group of reads, report the original reads along with the consensus | ||
-h, --help Print help | ||
``` | ||
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## Output format | ||
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TODO | ||
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## Options | ||
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- `--duplicates-only`: By default, _nailpolish_ will consensus call all duplicated reads and also produces each | ||
non-duplicated read intact. With this flag, only the duplicated reads will be produced, and all non-duplicated reads | ||
will be skipped. | ||
- `--report-original-reads`: By default, when reads are consensus called, only the consensus read is produced. | ||
With this flag, each of the original reads will be produced as well. |
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# nailpolish summary | ||
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Quickly review the quality and duplicate rate of the dataset. | ||
The reads must first have been [indexed](./generate-index.md). | ||
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## Usage | ||
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```shell | ||
$ nailpolish summary --help | ||
Generate a summary of duplicate statistics from an index file | ||
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Usage: nailpolish summary [OPTIONS] --index <INDEX> | ||
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Options: | ||
--index <INDEX> the index file | ||
--output <OUTPUT> output file [default: summary.html] | ||
-h, --help Print help | ||
``` | ||
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## Output | ||
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[See an example summary output file.](../assets/summary.html) | ||
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<iframe src="../assets/summary.html" style="width: 100%; height: 60vh; min-height: 500px;"></iframe> |
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