Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

CpG Methylation Calculation from bam file #43

Open
Irfanwustl opened this issue Sep 17, 2020 · 0 comments
Open

CpG Methylation Calculation from bam file #43

Irfanwustl opened this issue Sep 17, 2020 · 0 comments

Comments

@Irfanwustl
Copy link

Hi,
I am trying to understand which read to consider file for methylation value calculation from the bam file generated by BISCUIT? Also how to figure out which is "C strand" and "G strand" from the generated bam? For example, in the following biscuit tview image, there are 14 non duplicated reads (all has mapq=60) for the highlighted CpG.

biscuit

However, the generated bedgraph file is saying that the total depth is 10.
Screen Shot 2020-09-17 at 3 18 26 PM

Will you please explain what I am missing here? I have also attached the corresponding sample sam file (for some reason can not attach the bam file) if you want to reproduce it. Thanks

corres_pos_cgr1-1222701_nodup.txt

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant