MATLAB script to annotate polysomes in cellular tomograms based on ribosome coordinate and rotation information from subtomo analysis/averaging.
This script was first described in the manuscript by Xue et al, 2021. Preprint on bioRxiv.
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A motivelist(motl) table to combine and store all relevant information for each ribosomes, such as coordinates, rotations, class info, etc.
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Use ribosomes' mRNA exit to entry distance to define polysomes and their sequence.
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Extract quantitative information on polysome arrangement, such as overall distribution, relative orientations, etc.
Require MATLAB 2016b or equivalent.
Put the two associated fromEuler_RELION.m and rotmat2eulang.m files in MATLAB search Path.
Open the polysome_detect.m, and run it step by step (select and F9).