diff --git a/.changeset/lemon-beans-admire.md b/.changeset/lemon-beans-admire.md
new file mode 100644
index 000000000000..d593bd74340f
--- /dev/null
+++ b/.changeset/lemon-beans-admire.md
@@ -0,0 +1,5 @@
+---
+'@astrojs/db': patch
+---
+
+Fixes a bug where `astro:db:seed` couldn't access to the environment variable `ASTRO_DATABASE_FILE`
diff --git a/packages/db/src/core/integration/vite-plugin-db.ts b/packages/db/src/core/integration/vite-plugin-db.ts
index 29e98222ea87..7e10699df70a 100644
--- a/packages/db/src/core/integration/vite-plugin-db.ts
+++ b/packages/db/src/core/integration/vite-plugin-db.ts
@@ -124,12 +124,13 @@ export function getLocalVirtualModContents({
 	tables: DBTables;
 	root: URL;
 }) {
+	const { ASTRO_DATABASE_FILE } = getAstroEnv();
 	const dbInfo = getRemoteDatabaseInfo();
 	const dbUrl = new URL(DB_PATH, root);
 	return `
 import { asDrizzleTable, createLocalDatabaseClient, normalizeDatabaseUrl } from ${RUNTIME_IMPORT};
 
-const dbUrl = normalizeDatabaseUrl(import.meta.env.ASTRO_DATABASE_FILE, ${JSON.stringify(dbUrl)});
+const dbUrl = normalizeDatabaseUrl(${JSON.stringify(ASTRO_DATABASE_FILE)}, ${JSON.stringify(dbUrl)});
 export const db = createLocalDatabaseClient({ dbUrl, enableTransactions: ${dbInfo.url === 'libsql'} });
 
 export * from ${RUNTIME_VIRTUAL_IMPORT};