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Hi Wouter,
we have noticed that the length violin plots do not necessarily fit with the Length Histograms when using several samples. Plots were created using v.1.22.0 but I tried the latest version and get the same results.
Here is an example showing the violin plots (the highlighted sample is also the one show in the next plot:
And here is the corresponding histogramm with all samples except only the circled one is shown:
Here we would expect a peak at 10Kb. And if I run NanoComp on only that one sample it seems to be doing it right:
Do you have any idea on what might be wrong or suggestions if I did something wrong? I am running it pretty vanilla NanoComp --outdir ./output --fastq *_ont.fastq.gz
The text was updated successfully, but these errors were encountered:
Hi Wouter,
we have noticed that the length violin plots do not necessarily fit with the Length Histograms when using several samples. Plots were created using v.1.22.0 but I tried the latest version and get the same results.
Here is an example showing the violin plots (the highlighted sample is also the one show in the next plot:
![image](https://private-user-images.githubusercontent.com/32764481/251128458-bcc9a4a9-3917-4111-ac0d-256dbcaf16ae.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.J6g1_UdjQK8kVAm1p8I0UGCBg5dOt0WbU6oVr67Fc10)
And here is the corresponding histogramm with all samples except only the circled one is shown:
![image](https://private-user-images.githubusercontent.com/32764481/251128705-24ecbeaf-0e4c-4f79-a6df-b363a9a51296.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.FM1rIkEzHjDeDHNwnM5A-emM9kN_WCaN80480or7pWc)
Here we would expect a peak at 10Kb. And if I run NanoComp on only that one sample it seems to be doing it right:
![image](https://private-user-images.githubusercontent.com/32764481/251129499-a0b283c0-e8c2-4839-af80-dad59dfb5795.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.qid2YIDqCHBZqEOD3MxOyEpXPXTd1vWoVcB7XCRXK0U)
![image](https://private-user-images.githubusercontent.com/32764481/251130253-77ccdd6c-42e0-4bbb-aa39-c128559083f2.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.3f9T2fqYkH-qSA95CAtnJywoqwFQKMYY7DoC-2G2R90)
Do you have any idea on what might be wrong or suggestions if I did something wrong? I am running it pretty vanilla
NanoComp --outdir ./output --fastq *_ont.fastq.gz
The text was updated successfully, but these errors were encountered: