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PrimerDesign.jar not being created #5

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DanBeaton opened this issue Mar 11, 2021 · 0 comments
Open

PrimerDesign.jar not being created #5

DanBeaton opened this issue Mar 11, 2021 · 0 comments

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@DanBeaton
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Hello
Following the instructions for installing EcoFunPrimer and RDPTools, the PrimerDesign.jar is not being created in the PrimerDesign/dist folder as is described in the instructions.

A jar file called EcoFunPrimer.jar is installed in the PrimerDesign/dist folder and libPrimer3.so file with a lib directory.

Separately, installing MetaFunPrimer, a PrimerDesign.jar is created inside the MetaFunPrimer/src directory, not the RDPTools/PrimerDesign/dist directory, so I could test the instruction: java -Xmx8g -jar /path/to/PrimerDesign.jar [--help].

The output is

ERROR: Program needs os type, used default: mac
ERROR: Program needs output file directory to start
ERROR: Program needs sequence input fasta file to start
Exception in thread "main" java.lang.NullPointerException
at edu.msu.cme.rdp.readseq.utils.SeqUtils.guessFileFormat(SeqUtils.java:326)
at edu.msu.cme.rdp.readseq.utils.SeqUtils.getSeqReaderCore(SeqUtils.java:261)
at edu.msu.cme.rdp.readseq.readers.SequenceReader.(SequenceReader.java:38)
at edu.msu.cme.rdp.readseq.readers.SequenceReader.readFully(SequenceReader.java:75)
at edu.msu.cme.rdp.primerdesign.selectprimers.SelectPrimerPipeline.main(SelectPrimerPipeline.java:68)
at edu.msu.cme.rdp.primerdesign.selectprimers.PrimerDesign.main(PrimerDesign.java:233)

In contract, the installed EcoFunPrimer.jar out put is

java -Xmx8g -jar EcoFunPrimer.jar [--help]
USAGE: java -jar EcoFunPrimer.jar
screen - Screen for primer regions with for the best coverage at each position
select - Select primer pairs using slidescale or user defined fwd and rev primer positions

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