diff --git a/mypy/config_parser.py b/mypy/config_parser.py
index a0f93f663522..76396168550c 100644
--- a/mypy/config_parser.py
+++ b/mypy/config_parser.py
@@ -608,6 +608,11 @@ def parse_mypy_comments(
         # method is to create a config parser.
         parser = configparser.RawConfigParser()
         options, parse_errors = mypy_comments_to_config_map(line, template)
+
+        if "python_version" in options:
+            errors.append((lineno, "python_version not supported in inline configuration"))
+            del options["python_version"]
+
         parser["dummy"] = options
         errors.extend((lineno, x) for x in parse_errors)
 
diff --git a/test-data/unit/check-inline-config.test b/test-data/unit/check-inline-config.test
index bedba811d95b..c81dcac94afd 100644
--- a/test-data/unit/check-inline-config.test
+++ b/test-data/unit/check-inline-config.test
@@ -323,3 +323,7 @@ class Foo:
 foo = Foo()
 if foo: ...
 42 + "no"  # type: ignore
+
+
+[case testInlinePythonVersion]
+# mypy: python-version=3.10  # E: python_version not supported in inline configuration