diff --git a/README.md b/README.md index 3933b09..4ac72d7 100644 --- a/README.md +++ b/README.md @@ -322,12 +322,17 @@ quite light and usually start running as soon as they are scheduled. ### 5e) Known limitations * **Swapping if the number of queries too high.** If the number of queries is - too high, the auxiliary Python scripts start to use too much memory, which + too high (e.g., 10M Illumina reads), + the auxiliary Python scripts start to use too much memory, which may result in swapping. Try to keep the number of queries moderate and ideally their names short. * **No support for ambiguous characters in queries.** As the tools used internally by MOF-Search support only the nucleotide alphabet, all non-ACGT characters in queries are first converted to A. +* **Too many reported hits.** When queries have too many equally good hits in + the database, even if the threshold on the maximum number of hits is chosen + low – for instance 10 – the program will take top 10 + ties, which can be a + huge number (especially for short sequences). ## 6. License