diff --git a/tests/unit/test_io_hdf5_streaming.py b/tests/unit/test_io_hdf5_streaming.py
index d1c9d1ab3..0b146d413 100644
--- a/tests/unit/test_io_hdf5_streaming.py
+++ b/tests/unit/test_io_hdf5_streaming.py
@@ -89,6 +89,12 @@ def test_basic_read(self):
         with get_hdf5io(s3_path, "r", manager=self.manager, driver="ros3") as io:
             io.read()
 
+    def test_basic_read_with_aws_region(self):
+        s3_path = "https://dandiarchive.s3.amazonaws.com/blobs/11e/c89/11ec8933-1456-4942-922b-94e5878bb991"
+
+        with get_hdf5io(s3_path, "r", manager=self.manager, driver="ros3", aws_region="us-east-2") as io:
+            io.read()
+
 # Util functions and classes to enable loading of the NWB namespace -- see pynwb/src/pynwb/spec.py