From 5fa65976eced9c977301be223b4a228137af1da2 Mon Sep 17 00:00:00 2001 From: Jethro Rainford Date: Wed, 7 Jul 2021 16:44:14 +0100 Subject: [PATCH] readme update --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 563d98d5..f8201a5a 100644 --- a/README.md +++ b/README.md @@ -37,7 +37,7 @@ As a minimum, Athena requires 3 input files. These are a bed file for the gene p - transcript file: `chromosome start end gene transcript exon` - coverage file: `chromosome start end coverage` -n.b. the process for creating the transcript file may be found [here][transcript-file-url] +n.b. the process for creating the transcript file may be found [here][transcript-file-url]. ### Annotating BED file The BED file containing regions of interest is first required to be annotated with gene, exon and coverage information prior to analysis. This may be done using [BEDtools intersect][bedtools-intersect-url], with a file containing transcript to gene and exon information, and then the per base coverage data. Currently, 100% overlap is required between coordinates in the panel bed file and the transcript annotation file, therefore you must ensure any added flank regions etc. are the same.