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Hello, hicexplorer is amazing! I successfully used it to to analyze A/B compartment and call TADs, it was really helpful, but i encountered a problem recently. For example, as the pictures show, TADs were not called on some area of seveal chromsomes in different species.
I think it is not the problem of pygenometrack, because I checked the TAD_boundaries.bed and TAD_domains.bed, the relevant information is indeed missing for these areas, for example, the length of chromsome in first picture is 19032923bp, the domain information of this chromsome is below:
The version of hicexplorer i used is the latest one 3.7.6 installed by conda, and the version of python is 3.8.
The code I used and the feedback are listed below:
INFO:pygenometracks.tracksClass:initialize 1. [x-axis]
INFO:pygenometracks.tracksClass:initialize 2. [hic matrix]
WARNING:hicmatrix.HiCMatrix:Bin size is not homogeneous. Median 50000.000000
INFO:pygenometracks.tracksClass:initialize 3. [tads]
100%|█████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| 17/17 [00:00<00:00, 13850.65it/s]
INFO:pygenometracks.tracksClass:time initializing track(s):
INFO:pygenometracks.tracksClass:2.6904544830322266
DEBUG:pygenometracks.tracksClass:Figure size in cm is 40 x 2.6697970769549277. Dpi is set to 72
INFO:pygenometracks.tracksClass:plotting 1. [x-axis]
INFO:pygenometracks.tracksClass:plotting 2. [hic matrix]
WARNING:pygenometracks.tracks.GenomeTrack:*Warning*
The region to plot extends beyond the chromosome size. Please check.
Chr11 size: 19032923. Region to plot 0-49610884
INFO:pygenometracks.tracks.GenomeTrack:setting min, max values for track 2. [hic matrix] to: 1.0, 50.0
INFO:pygenometracks.tracksClass:plotting 3. [tads]
DEBUG:pygenometracks.tracks.GenomeTrack:ylim (0.0, 900000.0)
Meantime, i also found that althought other chromsomes seemed normal, but both ends of these are clear, Can hicexplorer not identify TADs at both ends of the chromosome?
Thank you very much for your time and assistance. I look forward to your guidance on this matter.
best
The text was updated successfully, but these errors were encountered:
Hello, hicexplorer is amazing! I successfully used it to to analyze A/B compartment and call TADs, it was really helpful, but i encountered a problem recently. For example, as the pictures show, TADs were not called on some area of seveal chromsomes in different species.
I think it is not the problem of pygenometrack, because I checked the TAD_boundaries.bed and TAD_domains.bed, the relevant information is indeed missing for these areas, for example, the length of chromsome in first picture is 19032923bp, the domain information of this chromsome is below:
The version of hicexplorer i used is the latest one 3.7.6 installed by conda, and the version of python is 3.8.
The code I used and the feedback are listed below:
Meantime, i also found that althought other chromsomes seemed normal, but both ends of these are clear, Can hicexplorer not identify TADs at both ends of the chromosome?
Thank you very much for your time and assistance. I look forward to your guidance on this matter.
best
The text was updated successfully, but these errors were encountered: