diff --git a/dandi/consts.py b/dandi/consts.py
index 59b422165..34391185a 100644
--- a/dandi/consts.py
+++ b/dandi/consts.py
@@ -167,7 +167,7 @@ def urls(self) -> Iterator[str]:
 VIDEO_FILE_EXTENSIONS = [".mp4", ".avi", ".wmv", ".mov", ".flv", ".mkv"]
 VIDEO_FILE_MODULES = ["processing", "acquisition"]
 
-ZARR_EXTENSIONS = [".ngff", ".zarr", ".nwb"]
+ZARR_EXTENSIONS = [".ngff", ".zarr"]
 
 #: Maximum allowed depth of a Zarr directory tree
 MAX_ZARR_DEPTH = 7