diff --git a/compose/cryostat.yml b/compose/cryostat.yml
index e70122f27..24e509f92 100644
--- a/compose/cryostat.yml
+++ b/compose/cryostat.yml
@@ -10,6 +10,7 @@ services:
       - ${XDG_RUNTIME_DIR}/podman/podman.sock:/run/user/1000/podman/podman.sock:Z
       - jmxtls_cfg:/truststore:U
       - templates:/opt/cryostat.d/templates.d:U
+      - probes:/opt/cryostat.d/probes.d:U
     security_opt:
       - label:disable
     hostname: cryostat
@@ -50,3 +51,5 @@ volumes:
     external: true
   templates:
     external: true
+  probes:
+    external: true
diff --git a/smoketest.bash b/smoketest.bash
index f6e2fd7d4..87ad9fc2b 100755
--- a/smoketest.bash
+++ b/smoketest.bash
@@ -266,6 +266,8 @@ cleanup() {
     ${container_engine} volume rm jmxtls_cfg || true
     ${container_engine} rm templates_helper || true
     ${container_engine} volume rm templates || true
+    ${container_engine} rm probes_helper || true
+    ${container_engine} volume rm probes || true
     truncate -s 0 "${HOSTSFILE}"
     for i in "${PIDS[@]}"; do
         kill -0 "${i}" && kill "${i}"
@@ -334,6 +336,15 @@ createEventTemplateVolume() {
 }
 createEventTemplateVolume
 
+createProbeTemplateVolume() {
+    "${container_engine}" volume create probes
+    "${container_engine}" container create --name probes_helper -v probes:/probes registry.access.redhat.com/ubi9/ubi-micro
+    if [ -d "${DIR}/probes" ]; then
+        "${container_engine}" cp "${DIR}/probes" probes_helper:/probes
+    fi
+}
+createProbeTemplateVolume
+
 setupUserHosts() {
     # This requires https://github.com/figiel/hosts to work. See README.
     truncate -s 0 "${HOSTSFILE}"
diff --git a/src/main/java/io/cryostat/ConfigProperties.java b/src/main/java/io/cryostat/ConfigProperties.java
index b117d3965..322cf74be 100644
--- a/src/main/java/io/cryostat/ConfigProperties.java
+++ b/src/main/java/io/cryostat/ConfigProperties.java
@@ -57,6 +57,7 @@ public class ConfigProperties {
 
     public static final String CUSTOM_TEMPLATES_DIR = "templates-dir";
     public static final String PRESET_TEMPLATES_DIR = "preset-templates-dir";
+    public static final String PROBE_TEMPLATES_DIR = "probe-templates-dir";
     public static final String SSL_TRUSTSTORE_DIR = "ssl.truststore.dir";
     public static final String RULES_DIR = "rules-dir";
 
diff --git a/src/main/java/io/cryostat/jmcagent/S3ProbeTemplateService.java b/src/main/java/io/cryostat/jmcagent/S3ProbeTemplateService.java
index a22588846..f72b73263 100644
--- a/src/main/java/io/cryostat/jmcagent/S3ProbeTemplateService.java
+++ b/src/main/java/io/cryostat/jmcagent/S3ProbeTemplateService.java
@@ -18,6 +18,8 @@
 import java.io.IOException;
 import java.io.InputStream;
 import java.nio.charset.StandardCharsets;
+import java.nio.file.Files;
+import java.nio.file.Path;
 import java.util.ArrayList;
 import java.util.List;
 import java.util.Map;
@@ -58,6 +60,9 @@ public class S3ProbeTemplateService implements ProbeTemplateService {
     @ConfigProperty(name = ConfigProperties.AWS_BUCKET_NAME_PROBE_TEMPLATES)
     String bucket;
 
+    @ConfigProperty(name = ConfigProperties.PROBE_TEMPLATES_DIR)
+    Path dir;
+
     @Inject S3Client storage;
     @Inject StorageBuckets storageBuckets;
 
@@ -74,6 +79,36 @@ public class S3ProbeTemplateService implements ProbeTemplateService {
 
     void onStart(@Observes StartupEvent evt) {
         storageBuckets.createIfNecessary(bucket);
+        if (!checkDir()) {
+            return;
+        }
+        try {
+            Files.walk(dir)
+                    .filter(Files::isRegularFile)
+                    .filter(Files::isReadable)
+                    .forEach(
+                            path -> {
+                                try (var is = Files.newInputStream(path)) {
+                                    logger.debugv(
+                                            "Uploading probe template from {0} to S3",
+                                            path.toString());
+                                    addTemplate(is, path.toString());
+                                } catch (IOException | SAXException e) {
+                                    logger.error(e);
+                                } catch (DuplicateProbeTemplateException e) {
+                                    logger.warn(e);
+                                }
+                            });
+        } catch (IOException e) {
+            logger.warn(e);
+        }
+    }
+
+    private boolean checkDir() {
+        return Files.exists(dir)
+                && Files.isReadable(dir)
+                && Files.isExecutable(dir)
+                && Files.isDirectory(dir);
     }
 
     @Override
@@ -187,6 +222,11 @@ public ProbeTemplate addTemplate(InputStream stream, String fileName)
             ProbeTemplate template = new ProbeTemplate();
             template.setFileName(fileName);
             template.deserialize(stream);
+            var existing = getTemplates();
+            if (existing.stream()
+                    .anyMatch(t -> Objects.equals(t.getFileName(), template.getFileName()))) {
+                throw new DuplicateProbeTemplateException(template.getFileName());
+            }
             storage.putObject(
                     PutObjectRequest.builder()
                             .bucket(bucket)
@@ -243,4 +283,10 @@ private Tagging createTemplateTagging(
                         .toList());
         return Tagging.builder().tagSet(tags).build();
     }
+
+    static class DuplicateProbeTemplateException extends IllegalArgumentException {
+        DuplicateProbeTemplateException(String templateName) {
+            super(String.format("Probe Template with name \"%s\" already exists", templateName));
+        }
+    }
 }
diff --git a/src/main/resources/application.properties b/src/main/resources/application.properties
index c9c684f94..7d05159d3 100644
--- a/src/main/resources/application.properties
+++ b/src/main/resources/application.properties
@@ -53,6 +53,7 @@ cryostat.agent.tls.required=true
 conf-dir=/opt/cryostat.d
 rules-dir=${conf-dir}/rules.d
 templates-dir=${conf-dir}/templates.d
+probe-templates-dir=${conf-dir}/probes.d
 preset-templates-dir=${conf-dir}/presets.d
 ssl.truststore=${conf-dir}/truststore.p12
 ssl.truststore.dir=/truststore