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+# Index Generation
+
+### Directory overview:
+
+#### **index-generation.wdl**:
+workflow code to create the following assets:
+* NT and NR indexes from NCBI with redundant sequences removed - we use this to develop additional indexes (notebaly minimap and diamond indexes).
+  Subsequently, we compile a "short DB" comprising solely the accessions that were identified from non-host alignment. This database is then used
+  to blast (blastx for NR blastn for NT) assembled contigs during the mNGS postprocessing phase.
+ 
+    * By using blastx we translates nucleotide hits from NT into protein sequences using the three different reading frames, we then search this sequence against the protein database (NR in this case). We also get out alignment statistics which we display in the mNGS sample report.
+    * By using blastn we can find regions of similarity between nucleotide sequences (in this case our contigs and NT). We also get out alignment statistics which we display in the mNGS sample report.
+          
+* nt_loc.marisa and nr_loc.marisa - index to quickly access an accession and it's sequence from either NT or NR.
+* nt_info.marisa and nr_info.marisa - index to quickly go from accession ID to accession name, sequence length, and offset information from either NT and NR
+* Minimap indexes - chunked minimap index for non host alignment for NT
+* diamond indexes - chunked diamond index for non host alignment for NR
+* accession2taxid.marisa - index to quickly go from accession ID to taxon ID - used for determining the optimal taxon assignment for each read from the alignment
+   results
+* taxid-lineages.marisa - index used to go from tax ID to taxonomy IDs (taxid for species, genus, family) - used for determining the optimal taxon assignment for each read from the alignment
+   results (calling hits), for example if a read aligns to multiple distinct references, we need to assess at which level in the taxonomic hierarchy the multiple alignments reach consensus. We also use this file for generating taxon counts. 
+   
+#### **ncbi-compress**: 
+compression code written in rust to remove redundant sequences from NT and NR
+
+#### **helpful_for_analysis**
+jupyter notebooks used for helpful common analysis steps including:
+* querying NCBI for an accession and lineage (used to investigate reads in the "all taxa with neither family nor genus classification" report for mNGS)
+* querying marisa trie files - notebook to easily query all marisa trie files generated from index generation workflow
+* compare non host alignment times between two projects - this was used to benchmark how long it took to do non host alignment on old and new indexes.
+* generate taxon lineage changelog for a sample report - get a readout on which reads from a sample report have a taxon / lineage change between new and old index runs. Used for comp bio validation purposes mainly.
+* checking sequence retention for different index compression runs - this notebook was handy for running multiple compression runs and summarizing which reads were dropped, helpful for early analysis and benchmarking of compression workflow.
+
+#### **ncbitax2lin.py**
+used to generate taxid-lineages.marisa 
+
+#### **generate_accession2taxid.py**
+used to generate accession2taxis.marisa
+
+#### **generate_ncbi_db_index.py**
+used to generate nt_loc.marisa and nr_loc.marisa
+
+#### **generate_lineage_csvs.py**
+used to generate versioned-taxid-lineages.csv file used to populate taxon_lineage database table, also generates changelogs for deleted taxa, changed taxa, and new taxa.