diff --git a/homeassistant/package_constraints.txt b/homeassistant/package_constraints.txt
index 953049da035f58..278c7ef6d4d914 100644
--- a/homeassistant/package_constraints.txt
+++ b/homeassistant/package_constraints.txt
@@ -145,7 +145,3 @@ matplotlib==3.6.1
 # pyOpenSSL 23.0.0 or later required to avoid import errors when
 # cryptography 39.0.0 is installed with botocore
 pyOpenSSL>=23.0.0
-
-# virtualenv 20.18 only compatible with <3
-# https://github.com/pypa/virtualenv/issues/2499
-platformdirs<3
diff --git a/script/gen_requirements_all.py b/script/gen_requirements_all.py
index e55479de8ea938..4456a3312b5f41 100755
--- a/script/gen_requirements_all.py
+++ b/script/gen_requirements_all.py
@@ -153,10 +153,6 @@
 # pyOpenSSL 23.0.0 or later required to avoid import errors when
 # cryptography 39.0.0 is installed with botocore
 pyOpenSSL>=23.0.0
-
-# virtualenv 20.18 only compatible with <3
-# https://github.com/pypa/virtualenv/issues/2499
-platformdirs<3
 """
 
 IGNORE_PRE_COMMIT_HOOK_ID = (