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Flag high coefficient of variation #465

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jakebeal opened this issue May 20, 2019 · 0 comments
Open

Flag high coefficient of variation #465

jakebeal opened this issue May 20, 2019 · 0 comments

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@jakebeal
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Steve Haase is looking for flags when a sample has a high coefficient of variation, which can indicate machine failures, such as air bubbles and malfunctioning fluidics.

Some key questions to resolve in order to define this well are:

  • Is this CV in arithmetic or geometric statistics?
  • Is this an absolute threshold or relative to other samples? (e.g., CV >50% higher than median sample)
  • Is this on certain channels, on every individual channel, or jointly across channels?
  • Should this be a parametric measure, when dealing with transient transfections that naturally have a wide range?
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