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curl of hg38bundle.tar.gz doesn't work #2

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rgiannico opened this issue Oct 3, 2018 · 4 comments
Open

curl of hg38bundle.tar.gz doesn't work #2

rgiannico opened this issue Oct 3, 2018 · 4 comments

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@rgiannico
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Hi Mark,
I'm so sorry to bother you again, but I just found this command doesn't work

$ curl -o external_data/grc38.tar.gz ftp://[email protected]/bundle/hg38/hg38bundle.tar.gz
  % Total    % Received % Xferd  Average Speed   Time    Time     Time  Current
                                 Dload  Upload   Total   Spent    Left  Speed
  0     0    0     0    0     0      0      0 --:--:--  0:00:01 --:--:--     0
curl: (78) RETR response: 550

And it's because the file is no more available if you look at the ftp site: ftp://ftp.broadinstitute.org/bundle/hg38/

This of course affects both the Dockerfile and the standard installation instructions.
Do you have any idea how to solve this?

@ProzacR
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ProzacR commented Nov 19, 2018

I tried to install it and face exactly same issue.

@whaleyr
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whaleyr commented Nov 20, 2018

The Broad Institute has reorganized their FTP server and we're having a hard time finding the equivalent file to the one used in the script.

If you find the equivalent file please post the URL here and we'll update the script. Otherwise, we post a fix if/when we can find it ourselves.

@mwaldron104
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I have a researcher that wants to use this tool, but there's still this issue of the hg38bundle.tar.gz file not being available. Has no one been able to locate it in a year? I sent a message to the Broad Institute, but received no reply.

@wbgalvao
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I have a researcher that wants to use this tool, but there's still this issue of the hg38bundle.tar.gz file not being available. Has no one been able to locate it in a year? I sent a message to the Broad Institute, but received no reply.

For anyone running into this problem, I was able to download the reference files from Broad's hg38 bundle connecting to their ftp server (ftp.broadinstitute.org). You can use gsapubftp-anonymous as username and your email address as password to login "anonymously".

There you can find the \bundle\hg38\ directory with all the reference files needed for the pipeline. Note that the Homo_sapiens_assembly38.known_indels.vcf.gz is under the beta directory.

If you want to do this from the command line you can run the following command:

wget ftp://gsapubftp-anonymous:`[email protected]`@ftp.broadinstitute.org/bundle/hg38/<target-file>

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