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Hi, I am running lima on PacBio MAS-seq long-read scRNA-seq data. This is the command I used:
lima /home/jiayiwang/loggedfs_david_penton/data/kinnex/segmented/PB258_3_r0362_bcM0004-segmented.bam data/primer/10x_3kit_primers.fasta /home/jiayiwang/loggedfs_david_penton/result/preprocessing/primer_removal/PB258_3_r0362_bcM0004.fl.5p--3p.bam --no-reports --num-threads 4 --isoseq
This command works for my other samples/bams. However, it returns error for this specific sample:
terminate called after throwing an instance of 'std::length_error'
what(): cannot create std::vector larger than max_size()
Aborted (core dumped)
I am not sure what this error means. Is the number of reads too large? My bam file is 67GB. I tried --split-bam, however, the error persists. It will be great if you could help me with this.
Thanks very much in advance!
The text was updated successfully, but these errors were encountered:
Hi, I am running lima on PacBio MAS-seq long-read scRNA-seq data. This is the command I used:
lima /home/jiayiwang/loggedfs_david_penton/data/kinnex/segmented/PB258_3_r0362_bcM0004-segmented.bam data/primer/10x_3kit_primers.fasta /home/jiayiwang/loggedfs_david_penton/result/preprocessing/primer_removal/PB258_3_r0362_bcM0004.fl.5p--3p.bam --no-reports --num-threads 4 --isoseq
This command works for my other samples/bams. However, it returns error for this specific sample:
I am not sure what this error means. Is the number of reads too large? My bam file is 67GB. I tried
--split-bam
, however, the error persists. It will be great if you could help me with this.Thanks very much in advance!
The text was updated successfully, but these errors were encountered: