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Write wrapper to build MC3 reference directory #12

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kamichiotti opened this issue Feb 10, 2018 · 2 comments
Open

Write wrapper to build MC3 reference directory #12

kamichiotti opened this issue Feb 10, 2018 · 2 comments

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@kamichiotti
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  • Address writing permissions problem with /mc3_dev/mc3/util/bgzip.cwl and ./mc3_dev/mc3/util/dbsnp_index/dbsnp_index.cwl, then include in wrapper (as noted in separate Github issue)
  • Write *gzi file for human reference
  • Write *tbi files for the cosmic and dbsnp references
  • Write *fai file for Homo_sapiens_assembly19.fasta
  • Temporary addition of Broad Indelocator files from broad_mc3_vcfs.tar.gz for testing purposes.
  • Files to pull in:
    1000G_phase1.indels.hg19.sites.vcf
    1000G_phase1.indels.hg19.sites.vcf.idx
    b37_cosmic_v54_120711.vcf.gz
    centromere_hg19.bed
    dbsnp_132_b37.leftAligned.vcf.gz
    Homo_sapiens_assembly19.dict
    Homo_sapiens_assembly19.fasta
    Mills_and_1000G_gold_standard.indels.hg19.sites.vcf
    Mills_and_1000G_gold_standard.indels.hg19.sites.vcf.idx
@kamichiotti
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Move prep_ref subworkflow in mc3_variant.cwl to the reference directory workflow (better to store two references than decompressing reference for each sample).

@kamichiotti
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kamichiotti commented Mar 20, 2018

Include download and preparation of homo_sapiens_vep_86_GRCh37.tar.gz, ExAC_nonTCGA.r0.3.1.sites.vep.vcf.gz and dbNSFPv2.9.1.zip for VEP.

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