diff --git a/pkgs/development/python-modules/nose-exclude/default.nix b/pkgs/development/python-modules/nose-exclude/default.nix
deleted file mode 100644
index 81d4e1a3cb750..0000000000000
--- a/pkgs/development/python-modules/nose-exclude/default.nix
+++ /dev/null
@@ -1,29 +0,0 @@
-{
-  stdenv,
-  lib,
-  buildPythonPackage,
-  fetchPypi,
-  nose,
-}:
-
-buildPythonPackage rec {
-  pname = "nose-exclude";
-  version = "0.5.0";
-  format = "setuptools";
-
-  src = fetchPypi {
-    inherit pname version;
-    sha256 = "f78fa8b41eeb815f0486414f710f1eea0949e346cfb11d59ba6295ed69e84304";
-  };
-
-  propagatedBuildInputs = [ nose ];
-
-  # "OSError: AF_UNIX path too long" for darwin
-  doCheck = !stdenv.isDarwin;
-
-  meta = {
-    license = lib.licenses.lgpl21;
-    description = "Exclude specific directories from nosetests runs";
-    homepage = "https://github.com/kgrandis/nose-exclude";
-  };
-}
diff --git a/pkgs/top-level/python-aliases.nix b/pkgs/top-level/python-aliases.nix
index 49eec3d93d70f..53a63162091c6 100644
--- a/pkgs/top-level/python-aliases.nix
+++ b/pkgs/top-level/python-aliases.nix
@@ -307,6 +307,7 @@ mapAliases ({
   nose-cov = throw "nose-cov has been removed, it was archived and unmaintained since 2012"; # added 2024-05-21
   nose-cover3 = throw "nose-cover3 has been removed, it was using setuptools 2to3 translation feature, which has been removed in setuptools 58"; # added 2022-02-16
   nose-cprof = throw "nose-cprof has been removed since it has not been maintained for 7 years and there are no dependent packages"; # added 2024-05-21
+  nose-exclude = throw "nose-exclude has been removed since it has not been maintained since 2016"; # added 2024-05-21
   nose-randomly = throw "nose-randomly has been removed, it was archived and unmaintained since 2019"; # added 2024-05-22
   nose_progressive = throw "nose_progressive has been removed, it was using setuptools 2to3 translation feature, which has been removed in setuptools 58"; #added 2023-02-21
   nose_warnings_filters = nose-warnings-filters; # added 2024-01-07
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 150965f1d2052..49635b86b0abf 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -8859,8 +8859,6 @@ self: super: with self; {
 
   nose3 = callPackage ../development/python-modules/nose3 { };
 
-  nose-exclude = callPackage ../development/python-modules/nose-exclude { };
-
   nose-timer = callPackage ../development/python-modules/nose-timer { };
 
   nose-pattern-exclude = callPackage ../development/python-modules/nose-pattern-exclude { };