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1-2-engineered-human-myocardium.html
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<li class="has-sub"><a href="index.html#intro"><span class="toc-section-number">1</span> Introduction</a><ul>
<li><a href="1-1-need-for-better-therapeutics.html#need-for-better-therapeutics"><span class="toc-section-number">1.1</span> Need for better therapeutics</a></li>
<li><a href="1-2-engineered-human-myocardium.html#engineered-human-myocardium"><span class="toc-section-number">1.2</span> Engineered Human Myocardium</a></li>
<li class="has-sub"><a href="1-3-rna-sequencing.html#rna-sequencing"><span class="toc-section-number">1.3</span> RNA Sequencing</a><ul>
<li><a href="1-3-rna-sequencing.html#bulk-rna-seq"><span class="toc-section-number">1.3.1</span> Bulk RNA Seq</a></li>
<li><a href="1-3-rna-sequencing.html#single-cell-rna-seq"><span class="toc-section-number">1.3.2</span> Single-cell RNA Seq</a></li>
</ul></li>
<li><a href="1-4-computational-deconvolution.html#computational-deconvolution"><span class="toc-section-number">1.4</span> Computational deconvolution</a></li>
<li><a href="1-5-principal-component-analysis-pca.html#principal-component-analysis-pca"><span class="toc-section-number">1.5</span> Principal Component Analysis (PCA)</a></li>
<li><a href="1-6-rationale-for-the-current-work.html#rationale-for-the-current-work"><span class="toc-section-number">1.6</span> Rationale for the current work</a></li>
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<li><a href="2-aims-and-objectives.html#aims-and-objectives"><span class="toc-section-number">2</span> Aims and Objectives</a></li>
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<li><a href="4-results-and-discussion.html#results-and-discussion"><span class="toc-section-number">4</span> Results and Discussion</a></li>
<li><a href="5-conclusion-and-future-work.html#conclusion-and-future-work"><span class="toc-section-number">5</span> Conclusion and Future Work</a></li>
<li><a href="6-references.html#references"><span class="toc-section-number">6</span> References</a></li>
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<div id="engineered-human-myocardium" class="section level2">
<h2><span class="header-section-number">1.2</span> Engineered Human Myocardium</h2>
<p>Translation to clinics require a production protocol that is compliant with current good manufacturing practices (cGMP). Ths has already been established and optimized by the working group over the years <span class="citation">(Tiburcy et al. <a href="#ref-tiburcyDefinedEngineeredHuman2017">2017</a>)</span>. Briefly, iPSCs are differentiated to not only cardiomyocytes, but also supportive stromal cell population using serum-free, GMP-friendly media and protocols. These differentiated cells are then combined in a fixed, optimized ratio within a collagen matrix. This mixture is then pipetted onto circular casting molds to make EHM rings or onto other shapes of molds to obtain the desired output form of the EHM see <a href="1-2-engineered-human-myocardium.html#fig:patch-fig">1.1</a>. Ideally, several such EHMs would be stacked to make a muscle layer of optimum thickness and sutured onto the failing myocardium, which would then integrate and assist mechanically in pumping.
> write about characteristics of EHM
> maturity/ immaturity? more on protocol?
> get more images for patches</p>
<div class="figure" style="text-align: center"><span id="fig:patch-fig"></span>
<img src="data/Patch_Zimmermann_Feb_2017.png" alt="Various shapes and forms of EHMs for clinical and experimental applications" width="50%" />
<p class="caption">
Figure 1.1: Various shapes and forms of EHMs for clinical and experimental applications
</p>
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</div>
<h3> References</h3>
<div id="refs" class="references">
<div id="ref-tiburcyDefinedEngineeredHuman2017">
<p>Tiburcy, Malte, James E. Hudson, Paul Balfanz, Susanne Schlick, Tim Meyer, Mei-Ling Chang Liao, Elif Levent, et al. 2017. “Defined Engineered Human Myocardium with Advanced Maturation for Applications in Heart Failure Modelling and Repair.” <em>Circulation</em> 135 (19): 1832–47. <a href="https://doi.org/10.1161/CIRCULATIONAHA.116.024145">https://doi.org/10.1161/CIRCULATIONAHA.116.024145</a>.</p>
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