diff --git a/psi-ms.obo b/psi-ms.obo
index 3bdcddb..8c60892 100644
--- a/psi-ms.obo
+++ b/psi-ms.obo
@@ -1,5 +1,5 @@
 format-version: 1.2
-data-version: 4.1.188
+data-version: 4.1.189
 date: 13:01:2025 17:58
 saved-by: Joshua Klein
 default-namespace: MS
@@ -8495,6 +8495,15 @@ def: "Cleavage agent Trypsin/P." [PSI:PI]
 is_a: MS:1001045 ! cleavage agent name
 relationship: has_regexp MS:1001339 ! (?<=[KR])
 
+[Term]
+id: MS:1003434
+name: AlphaLP
+def: "A serine protease that hydrolyzes peptide bonds at the C-terminus of threonine, alanine, serine, and valine. (EC:3.4.21.12)" [DOI:10.1038/228438a0, DOI:10.1021/bi00445a015]
+is_a: MS:1001045 ! cleavage agent name
+synonym: "alpha-lytic endopeptidase" EXACT []
+synonym: "alpha-lytic protease" EXACT []
+relationship: has_regexp MS:1003435 ! (?<=[TASV])
+
 [Term]
 id: MS:1001314
 name: V8-DE
@@ -12865,6 +12874,12 @@ name: (?<=W)
 def: "Regular expression for 2-iodobenzoate." [PSI:PI]
 is_a: MS:1001180 ! Cleavage agent regular expression
 
+[Term]
+id: MS:1003435
+name: (?<=[TSAV])
+def: "Regular expression for alphaLP." [PSI:PI]
+is_a: MS:1001180 ! Cleavage agent regular expression
+
 [Term]
 id: MS:1001961
 name: peptide spectrum match scoring algorithm