Skip to content

Files

Latest commit

oospinaoospina
Update README
Jul 26, 2022
ffdab65 · Jul 26, 2022

History

History
38 lines (31 loc) · 2.52 KB

README.md

File metadata and controls

38 lines (31 loc) · 2.52 KB

A primer on pre-processing, visualization, clustering, and phenotyping of barcode-based spatial transcriptomics

By: Oscar Ospina, Alex Soupir, and Brooke L. Fridley
From: Statistical Genomics

This repository contains the code to create the figures in the book chapter "A primer on pre-processing, visualization, clustering, and phenotyping of barcode-based spatial transcriptomics", part of the book (under preparation) entitled "Statistical Genomics" (Eds: Drs. Brooke L. Fridley and Xuefeng Wang).

Data used to create the figures in this chapter are not included in this repository and will need to be downloaded by the reader from the following sources:

Figure 1:

Museum of Spatial Transcriptomics (by Moses and Pachter, 2022): Excel spreadsheet containing metadata on published articles and preprints. The code contains commands to directly download and read the data within R via googlesheets4. LINK TO PAPER DESCRIBING THE DATABASE.

Figures 3, 4, 5, 6, and 8:

Ductal breast carcinoma 10X Visium sample, available at the 10X data repository. Before download, registration to 10X Genomics may be required. To download the data, The reader can query "Human Breast Cancer Block A Section 2", select "Spatial Gene Expression", and then, select "Space Ranger 1.1.0". Clicking on the query result will take the reader to the sample download webpage. The code in this chapter uses the barcode matrices and the spatial imaging data. LINK TO 10X GENOMICS DATA REPOSITORY.

Figure 7:

Human kidney GeoMx sample, available at Nanostring's Spatial Organ Atlas website. Before download, registration to Nanostring may be required. To download the data, the reader can click on "Data Downloads" and then click on "Count Results". This link will produce a compressed .gz file, which must be decompressed by the reader. The resulting folder contains the Excel spreadsheet with ROI metadata and gene counts necessary to generate the chapter's figure. LINK TO NANOSTRING SPATIAL ORGAN ATLAS.

Figure 9:

Mouse kidney single-cell and spatial data sets from the R libraries TabulaMurisSenisData (Soneson et al. 2022) GITHUB and TENxVisiumData (Crowell, 2022) GITHUB. The code contains commands to directly download and read the data sets within R.