diff --git a/docs/tutorial/01_opt_external_models/Ch_External_Models.ipynb b/docs/tutorial/01_opt_external_models/Ch_External_Models.ipynb new file mode 100644 index 00000000..027afd11 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/Ch_External_Models.ipynb @@ -0,0 +1,2064 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "b365f293-55b6-467d-acdb-ec110988c8fd", + "metadata": {}, + "source": [ + "# Using Customized and External Cell and Channel Models in BioNet" + ] + }, + { + "cell_type": "markdown", + "id": "88d41f7b-4ff9-4f25-99fd-9bc0c0804fa5", + "metadata": {}, + "source": [ + "Through the main tutorials, we've been using networks built off cell models downloaded from the [Allen Cell-Types Database](https://celltypes.brain-map.org/data). While the Allen Cell-Types Databases has a comprehensive and continuingly growing selection of optimized cell-type models to download and use, for many scientists, it may not meet their needs. BMTK supports incorporating many cell types and synaptic models into their simulations, including pre-built models from non-Allen Institute organizations. It also allows users multiple ways to create their customized models. \n", + "\n", + "This section will focus on single-cell spiking models, including conductance-based models with multiple channels and/or compartments per model. We do not recommend using Rates-based models with BioNet, and they will likely not work.\n", + "\n", + "The following section will show users multiple ways to import or create their own networks and simulations. Many of the multi-compartment biophysically detailed models developed for the NEURON simulator are written in [.hoc](https://www.neuron.yale.edu/neuron/static/new_doc/programming/hocsyntax.html) format. However, another popular format is models written in [NeuroML v2](https://docs.neuroml.org/Userdocs/NeuroMLv2.html). In other cases, models may be written in Python. BioNet supports these options and allows users to readily implement other options, too.\n", + "\n", + "If you are unfamiliar with how and where to find cell and channel models that have been created by other scientists, two good options to start looking at are:\n", + "1. [ModelDB](https://modeldb.science/)\n", + "2. [Open Source Brain](https://v2.opensourcebrain.org/)\n", + "\n", + "\n", + "**Note** - scripts and files for running this tutorial can be found in the directory [01_opt_external_models/](https://github.com/AllenInstitute/bmtk/tree/develop/docs/tutorial/01_opt_external_models)" + ] + }, + { + "cell_type": "markdown", + "id": "668af9a7-054d-402f-9747-b164388f23ac", + "metadata": {}, + "source": [ + "---\n", + "\n", + "## Contents\n", + "\n", + "1. [Cell Models](#cell-models)\n", + "\n", + " 1.1. [Example: Loading a simple NEURON HOC template cell model into your network simulation](#example-simple-hoc)\n", + " \n", + " 1.2. [Example: Overriding the way which BMTK creates cell models before simulation](#example-modify-init)\n", + " \n", + " 1.3. [Example: Loading a python-based neuronal model into your simulation](#example-python-models)\n", + "\n", + "\n", + "2. [Modifying an existing cell model](#mod-existing-cell-model)\n", + "\n", + " 2.1. [Example: Building your own python-based cell models](#example-custom-cells)\n", + " \n", + " 2.2. [Example: Adding customized mechanisms into an existing cell model](#model-processing-added-mech)\n", + " \n", + " 2.3. [Example: Modifying channel conductance in an instantiated cell](#model-processing-channel-conductance)\n", + "---" + ] + }, + { + "cell_type": "markdown", + "id": "a08c7955-0ed7-427a-a7eb-dbf97e212cc8", + "metadata": {}, + "source": [ + "## 1. Cell Models " + ] + }, + { + "cell_type": "markdown", + "id": "406d9391-8865-44a6-ba50-16dc2df98a3b", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 1.1. Example: Loading in a Simple Hoc Model " + ] + }, + { + "cell_type": "markdown", + "id": "392eeeb2-95f7-44c6-a75f-6a2a8c20dc3c", + "metadata": {}, + "source": [ + "\n", + "##### Step 1: \n", + "\n", + "**Note**: *This step has already been done in the current notebook and does not need to be repeated, but may be useful when initializing other examples*\n", + "\n", + "The first thing we'll do is to download and unpack the model files from ModelDB, which for the Hay L5PC models can be found at [https://modeldb.science/139653](https://modeldb.science/139653). At the site, you can use the download button to download a zip file with the appropriate .hoc (cell model), .mod (ion channels), and .asc (morphologies) files.\n", + "\n", + "\n", + "
\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "To run properly, we will need to move the following files from the download zip file into our **components/** folder so BMTK will be able to find them during the simulation:\n", + "* Move **mod/** folder => **componets/mechanisms/mod/**.\n", + "* Move **morphologies/\\*.asc** files => **components/morphologies/** folder.\n", + "* Move **models/L5PCtemplate.hoc** and **models/L5PCbiophys4.hoc** => **componets/templates/** folder (*note*: The zip file contains four different version of L5PCbiophys model stored in different hoc files. However, only one can be loaded into NEURON at a time, hence we are only moving one of the files and not all of them).\n", + " \n" + ] + }, + { + "cell_type": "markdown", + "id": "b89a7c1e-8b28-4db5-bf96-9598dc3d8bb0", + "metadata": {}, + "source": [ + "The model contains customized ion channels in the **components/mechanisms/mod/** folder that require compiling using the `nrnivmod` command. In a shell or using jupyter notebook you'll need to run the following:" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "933ad3b3-d8ea-4b14-add0-554f3723571b", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "/home/shared/workspace/docs/tutorial/01_opt_external_models/components/mechanisms\n", + "Mod files: \"modfiles/CaDynamics.mod\" \"modfiles/CaDynamics_E2.mod\" \"modfiles/Ca_HVA.mod\" \"modfiles/Ca_LVA.mod\" \"modfiles/Ca_LVAst.mod\" \"modfiles/Cav2_1.mod\" \"modfiles/Cav3_1.mod\" \"modfiles/Cav3_2.mod\" \"modfiles/Cav3_3.mod\" \"modfiles/Gfluct.mod\" \"modfiles/HCN1_Angeloetal2007.mod\" \"modfiles/Ih.mod\" \"modfiles/Im.mod\" \"modfiles/Im_v2.mod\" \"modfiles/K_P.mod\" \"modfiles/K_Pst.mod\" \"modfiles/K_T.mod\" \"modfiles/K_Tst.mod\" \"modfiles/Kca11.mod\" \"modfiles/Kca22.mod\" \"modfiles/Kca31.mod\" \"modfiles/Kd.mod\" \"modfiles/Kir23.mod\" \"modfiles/Kv11.mod\" \"modfiles/Kv15.mod\" \"modfiles/Kv2like.mod\" \"modfiles/Kv33.mod\" \"modfiles/Kv34.mod\" \"modfiles/Kv3_1.mod\" \"modfiles/Kv43.mod\" \"modfiles/Leak.mod\" \"modfiles/NaTa.mod\" \"modfiles/NaTa_t.mod\" \"modfiles/NaTg.mod\" \"modfiles/NaTs.mod\" \"modfiles/NaTs2_t.mod\" \"modfiles/NaV.mod\" \"modfiles/Nap.mod\" \"modfiles/Nap_Et2.mod\" \"modfiles/Nav16.mod\" \"modfiles/PC_Gaba_det_vi_alfa1.mod\" \"modfiles/PURKINJE_Ampa_det_vi.mod\" \"modfiles/ProbAMPANMDA2.mod\" \"modfiles/ProbUDFsyn2.mod\" \"modfiles/SK.mod\" \"modfiles/SK_E2.mod\" \"modfiles/SKv3_1.mod\" \"modfiles/cdp5_CAM.mod\" \"modfiles/epsp.mod\" \"modfiles/exp1isyn.mod\" \"modfiles/exp1syn.mod\" \"modfiles/stp1syn.mod\" \"modfiles/stp2syn.mod\" \"modfiles/stp3syn.mod\" \"modfiles/stp4syn.mod\" \"modfiles/stp5isyn.mod\" \"modfiles/stp5syn.mod\" \"modfiles/vecevent.mod\"\n", + "\n", + "COBJS=''\n", + " -> \u001b[32mCompiling\u001b[0m mod_func.c\n", + "gcc -O2 -I. -I/opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/include -I/nrnwheel/openmpi/include -fPIC -c mod_func.c -o mod_func.o\n", + " => \u001b[32mLINKING\u001b[0m shared library ./libnrnmech.so\n", + "g++ -O2 -DVERSION_INFO='8.0.0' -std=c++11 -shared -fPIC -I /opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/include -o ./libnrnmech.so -Wl,-soname,libnrnmech.so \\\n", + " ./mod_func.o ./CaDynamics.o ./CaDynamics_E2.o ./Ca_HVA.o ./Ca_LVA.o ./Ca_LVAst.o ./Cav2_1.o ./Cav3_1.o ./Cav3_2.o ./Cav3_3.o ./Gfluct.o ./HCN1_Angeloetal2007.o ./Ih.o ./Im.o ./Im_v2.o ./K_P.o ./K_Pst.o ./K_T.o ./K_Tst.o ./Kca11.o ./Kca22.o ./Kca31.o ./Kd.o ./Kir23.o ./Kv11.o ./Kv15.o ./Kv2like.o ./Kv33.o ./Kv34.o ./Kv3_1.o ./Kv43.o ./Leak.o ./NaTa.o ./NaTa_t.o ./NaTg.o ./NaTs.o ./NaTs2_t.o ./NaV.o ./Nap.o ./Nap_Et2.o ./Nav16.o ./PC_Gaba_det_vi_alfa1.o ./PURKINJE_Ampa_det_vi.o ./ProbAMPANMDA2.o ./ProbUDFsyn2.o ./SK.o ./SK_E2.o ./SKv3_1.o ./cdp5_CAM.o ./epsp.o ./exp1isyn.o ./exp1syn.o ./stp1syn.o ./stp2syn.o ./stp3syn.o ./stp4syn.o ./stp5isyn.o ./stp5syn.o ./vecevent.o -L/opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/lib -lnrniv -Wl,-rpath,/opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/lib \n", + "rm -f ./.libs/libnrnmech.so ; mkdir -p ./.libs ; cp ./libnrnmech.so ./.libs/libnrnmech.so\n", + "Successfully created x86_64/special\n" + ] + } + ], + "source": [ + "! cd components/mechanisms && nrnivmodl modfiles" + ] + }, + { + "cell_type": "markdown", + "id": "9674554b-2bdb-4454-81d6-43cf6f9fe082", + "metadata": {}, + "source": [ + "##### Step 2: Building the Network\n", + "\n", + "Building a network using the downloaded L5PC Hay model is very similar to how we built biophysically detailed network models in [previous chapters](../Ch3_multicells/3.%20Multipopulation%20biophysical.ipynb). We use the `add_nodes()` method to add a population of 1 or more L5PC `biophysical` cells and the `add_edges()` to connect them up with other cells. However, due to the model type and parameters, we will need to make some changes compared to how we utilize Allen-Cell Type models.\n", + "\n", + "* We set **model_template**=`hoc:L5PCtemplate` instead of the Allen `ctdb:Biophys1` value. The name of the Hoc Template for loading this particular model comes from the components/templates/L5PCtemplate.hoc file\n", + "* In our Allen Cell-Types models, we had set the value **model_processing** to 'aibs_perisomatic', which told bmtk to call the special post-intialization directive `aibs_perisomatic`, which would modify each cell object in a specific manner. Although it is possible to create custom directives to update and modify each cell after the template has been loaded (see below), we don't want to do so. Instead, we leave the model_processing attribute blank.\n", + "* In our Allen Cell-Types models, we also had a **dynamics_params** option that would update and add cell parameters after the cell had been initialized. However, for our L5PC Hay model, we have no current need to update the parameters (we'll instead use the parameter values stored in the .hoc files), so we will not have any dynamics_params attributes.\n", + " " + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "ef2cc58c-6104-48ab-9c90-8c2ae3366597", + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "WARNING:root:No edges have been made for this network, skipping saving of edges file.\n" + ] + } + ], + "source": [ + "from bmtk.builder.networks import NetworkBuilder\n", + "\n", + "net = NetworkBuilder('L5')\n", + "net.add_nodes(\n", + " N=10,\n", + " model_type='biophysical',\n", + " model_template='hoc:L5PCtemplate',\n", + " morphology='cell3.asc'\n", + ")\n", + "net.build()\n", + "net.save(output_dir='network_L5')" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "0f5ce031-8c95-4d89-846e-cba00e282ab1", + "metadata": {}, + "outputs": [], + "source": [ + "virt_exc = NetworkBuilder('virt_exc')\n", + "virt_exc.add_nodes(\n", + " N=20,\n", + " model_type='virtual',\n", + " ei_type='exc'\n", + ")\n", + "conns = virt_exc.add_edges(\n", + " source=virt_exc.nodes(),\n", + " target=net.nodes(),\n", + " connection_rule=12,\n", + " model_template='Exp2Syn',\n", + " dynamics_params='AMPA_ExcToExc.json',\n", + " distance_range=[0.0, 1.0e20],\n", + " target_sections=['soma', 'basal', 'apical'],\n", + " delay=2.0,\n", + " syn_weight=0.01\n", + ")\n", + "\n", + "virt_exc.build()\n", + "virt_exc.save(output_dir='network_L5')" + ] + }, + { + "cell_type": "markdown", + "id": "630bbd89-4ba9-4878-be1e-11a7e90ad732", + "metadata": {}, + "source": [ + "##### Step 3: Running the simulation\n", + "\n", + "Running the simulation with the downloaded L5PC models is no different than running the simulations with the Allen Cell-Types Database cell models. BMTK will be able to use the configuration **components** sections along with the **model_template** and **morphology** values to automatically load in our 10 L5PC cells. The results for these cells, including spikes, Vm traces, etc., will be saved in the same format and paths as our other examples." + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "6a5659ff-4ec4-4f4a-afd2-16291b8b6738", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:09,287 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "numprocs=1\n", + "2024-10-21 18:38:09,412 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:16,890 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:16,902 [INFO] Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:16,982 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:16,984 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:16,986 [INFO] Block save every 5000 steps\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Block save every 5000 steps\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:30,278 [INFO] step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:43,513 [INFO] step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:38:56,897 [INFO] step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:10,212 [INFO] step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:10,249 [INFO] Simulation completed in 53.27 seconds \n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Simulation completed in 53.27 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.L5.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "83ef2b8f-4c81-48ec-b8f6-faa3a07cfaa7", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 1.2. Example: Modifying Model Initialization
" + ] + }, + { + "cell_type": "markdown", + "id": "0c24a66f-8db0-44b0-948e-c4dbf9f93210", + "metadata": {}, + "source": [ + "If we search Open Source Brain, we can see their own more recent versions of [L5 Pyramidal Cell models from the same lab](https://v2.opensourcebrain.org/repositories/2180). Suppose we wanted to incorporate these newer models alongside the original L5 PC network created above. We would need to do the following:\n", + "1. Download the [files](https://github.com/OpenSourceBrain/267587/tree/master/Single%20Cell%20Modelling/Current-Step%20Simulations/L5Pyr_young)\n", + "2. Move the morphology, mode, and model template files to their appropriate location\n", + "3. Since these files also include new .mod files, we would have to recompile them:\n", + "```bash\n", + " $ cd components/mechanisms && nrnivmodl modfiles\n", + "```\n", + "\n", + "The newer models have a different morphology file (`Hl5PN1.swc`) and HOC Template name (`HNTemplate`). So in order to add these new cells to the old one we can add the following call in our build script (**build_network.L5_updated.py**):\n", + "\n", + "```python\n", + "net.add_nodes(\n", + " N=5,\n", + " model_type='biophysical',\n", + " model_template='hoc:HNTemplate',\n", + " morphology='HL5PN1.swc'\n", + ")\n", + "```\n", + "\n", + "*Note: We'll also modify the script to write the network to a different directory*\n" + ] + }, + { + "cell_type": "markdown", + "id": "1d73fd45-d720-42d8-81da-7bb889872012", + "metadata": {}, + "source": [ + "We could try to run the **L5_updated** model like before, but it would throw an error\n", + "\n", + "
\n", + "⚠️ NEURON: init not enough arguments\n", + "
\n", + "\n", + "The Reason: **We are not calling the HNTemplate correctly.**" + ] + }, + { + "cell_type": "markdown", + "id": "cbae5da5-3592-4020-a4cb-51e53284cac7", + "metadata": {}, + "source": [ + "If we look through the code and the documentation, we can see that, to instantiate an HNTemplate cell model instance, we must pass two parameters: The morphology file and the cell-type (one of a preset number of string values that will determine how specific properties are set within the model). In this case, the call would look like:\n", + "\n", + "```python\n", + " h.HNTemplate(morphology_file_path, \"HL5PN1\")\n", + "```\n", + "\n", + "The built-in BMTK function that tries to load hoc files, [loadHOC](https://github.com/AllenInstitute/bmtk/blob/develop/bmtk/simulator/bionet/default_setters/cell_models.py#L43), does not know how to load this specific template. And even if it did, you may run into other models that require different, unseen ways of initialization. \n", + "\n", + "While it is possible to overwrite the `loadHOC` function, it is not ideal. To avoid breaking existing models, we would need to have multiple cases based on each possible template." + ] + }, + { + "cell_type": "markdown", + "id": "0dcb4381-0ca6-4460-8973-bac09438a69b", + "metadata": {}, + "source": [ + "**Solution**\n", + "\n", + "Instead of using one function to load in every template, we can create our own custom function to load the HNTemplate. To achieve this, BMTK uses a special [python decorator](https://peps.python.org/pep-0318/) to assign to function users have written (in either the notebook or run_bionet.py script). Below, we show an example of how to do this using a custom function we'll call loadHNTemplate():" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "861c2dfc-ce17-420b-b5e1-beb294996ff5", + "metadata": {}, + "outputs": [], + "source": [ + "from neuron import h\n", + "from bmtk.simulator.bionet import cell_model\n", + "from bmtk.simulator.bionet.io_tools import io\n", + "\n", + "@cell_model(directive='hoc:HNTemplate', model_type='biophysical')\n", + "def loadHNTemplate(cell, template_name, dynamics_params):\n", + " io.log_info(f'Calling loadHNTemplate() for cell {cell[\"node_id\"]}, template {template_name}')\n", + " hobj = h.HNTemplate(cell['morphology'], \"HL5PN1\")\n", + " return hobj" + ] + }, + { + "cell_type": "markdown", + "id": "8f99de13-a200-445e-b70a-bd2b3fbdd08e", + "metadata": {}, + "source": [ + " * The `@cell_model` is a decorator we put at the front of our function. Using `directive=hoc:Template` and `model_type=biophysical` parameters indicates to BMTK that whenever a cell model needs to be built for a *biophysical*, *HNTemplate* hoc template, it will use this function.\n", + " * Our function requires three parameters;\n", + " * The `cell` parameter contains attributes of each individual cell and can be used to extract properties like the morphology file, the node-id, coordinates, etc.\n", + " * The `template_name` is, in this case, just the string value \"HNTemplate\"\n", + " * The `dynamics_params` is a dictionary from the **dynamics_params** json or hdf5. In this network, we never set the dynamics_params attributes, so the value will be None.\n", + " * We need to return either a hoc-object or some other Python class built using the NEURON API.\n", + " * For a sanity check, we also included a log statement. We should only see this message for the 5 newer HNTemplate models, not for the other original \"L5PCTemplate\" cells.\n", + "\n", + "\n", + "During simulation, this function will be called for every cell with matching \"template_name\" and \"model_type\"." + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "id": "d315eb04-6c0f-415b-aedd-21a8c8253ba8", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:10,266 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-10-21 18:39:10,335 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:14,153 [INFO] Calling loadHNTemplate() for cell 5, template HNTemplate\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Calling loadHNTemplate() for cell 5, template HNTemplate\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:14,377 [INFO] Calling loadHNTemplate() for cell 6, template HNTemplate\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Calling loadHNTemplate() for cell 6, template HNTemplate\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:14,596 [INFO] Calling loadHNTemplate() for cell 7, template HNTemplate\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Calling loadHNTemplate() for cell 7, template HNTemplate\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:14,813 [INFO] Calling loadHNTemplate() for cell 8, template HNTemplate\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Calling loadHNTemplate() for cell 8, template HNTemplate\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:15,043 [INFO] Calling loadHNTemplate() for cell 9, template HNTemplate\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Calling loadHNTemplate() for cell 9, template HNTemplate\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:15,258 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:15,282 [INFO] Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:15,346 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:15,347 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:15,348 [INFO] Block save every 5000 steps\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Block save every 5000 steps\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:35,798 [INFO] step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:39:56,404 [INFO] step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:17,730 [INFO] step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:38,490 [INFO] step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:38,524 [INFO] Simulation completed in 83.18 seconds \n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Simulation completed in 83.18 seconds \n" + ] + } + ], + "source": [ + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.L5_updated.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "76b4dafc-4f86-4013-aee0-3188269e5a43", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 1.3. Example: Loading a python-based neuronal model into your simulation
" + ] + }, + { + "cell_type": "markdown", + "id": "fc168bf2-4487-40a7-96b0-b00ad0748336", + "metadata": {}, + "source": [ + "Often, rather than being written in old .hoc format, scientists may instead release their models written in Python. For example, in this [mouse Purkinje cell model](https://github.com/OpenSourceBrain/267694/tree/main/mouse_model), the model is itself saved in the `Purkinje_morph_1.Purkinje_Morph_1` Python class. In nearly the same manner as we did with the .hoc files above, we can import these Python-based single-cell models into our network to use with BMTK.\n", + "\n", + "As before, we must download the necessary files, but in this case, the *.py files, including the file containing the cell-model class/template, should be in the same directory as our `run_bionet.py` script so that we may import it. Also, the Python script expects the .asc morphology file to be hard-coded in the path **./morphology/soma_10c.asc**, so we'll put it there instead of the normal components directory (unless you want to edit the file).\n" + ] + }, + { + "cell_type": "markdown", + "id": "629ba385-8115-498c-bb3f-d900047ddba2", + "metadata": {}, + "source": [ + "When we build our population of Purkinje cells, the \"model_template\" will have schema value `python` and a value that is just the name of the resources, e.g., the python class `Purkinje_morph_1`.\n", + "\n", + "```\n", + "net.add_nodes( \n", + " pop_name='Scnn1a',\n", + " model_type='biophysical',\n", + " model_template='python:Purkinje_morph_1',\n", + " spines_on=0\n", + ")\n", + "```\n", + "\n", + "So that BMTK can load the template, we will once again register a special function using the `cell_model` decorator. The class can be built with or without dendritic spines, which we are required to pass in. The `__init__` method of `Purkinje_Morpho_1` will build the model for each given cell, which we will pass back to BTMK.\n", + "\n", + "Also, for good measure, we added some optional logging." + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "id": "4d416c2d-a536-408a-bd35-c476e846d547", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "from bmtk.simulator.bionet.io_tools import io\n", + "from Purkinje_morpho_1 import Purkinje_Morpho_1\n", + "\n", + "@bionet.cell_model(directive='python:Purkinje_morph_1', model_type='biophysical')\n", + "def loadPurkinjeModel(cell, template_name, dynamics_params):\n", + " io.log_info(f'Loading cell {cell.node_id}, tempalate {template_name}, with spines_on={cell[\"spines_on\"]}')\n", + " cell = Purkinje_Morpho_1(cell['spines_on'])\n", + " return cell" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "id": "004d316b-6f4f-4d99-b521-ece8c51c9bdc", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:38,556 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-10-21 18:40:38,613 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:38,614 [INFO] Loading cell 0, tempalate Purkinje_morph_1, with spines_on=0\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Loading cell 0, tempalate Purkinje_morph_1, with spines_on=0\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "\n", + "3856 lines read\n", + "indiv number 138\n", + "2024-10-21 18:40:38,784 [INFO] Loading cell 1, tempalate Purkinje_morph_1, with spines_on=1\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Loading cell 1, tempalate Purkinje_morph_1, with spines_on=1\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "\n", + "3856 lines read\n", + "indiv number 138\n", + "spine active!!\n", + "dend_total_len 7680\n", + "list_dend_complete 7680\n", + "somma_location_spines 7680\n", + "spines_placed 0\n", + "spines_placed 500\n", + "spines_placed 1000\n", + "spines_placed 1500\n", + "spines_placed 2000\n", + "spines_placed 2500\n", + "spines_placed 3000\n", + "spines_placed 3500\n", + "spines_placed 4000\n", + "spines_placed 4500\n", + "spines_placed 5000\n", + "spines_placed 5500\n", + "spines_placed 6000\n", + "spines_placed 6500\n", + "spines_placed 7000\n", + "spines_placed 7500\n", + "spines_syn: spine_head_0\n", + "spines_syn: spine_head_5000\n", + "spine_head_aa between 0 - 0.3 -> 0\n", + "spine_head_aa_SC between 0.3 - 0.75 -> 1494\n", + "spine_head_pf_SC between 0.75 - 1.5 -> 6184\n", + "!!!!!!!!!!!!!!! spine_total 7678 !!!!!!!!!!!!!!!\n", + "2024-10-21 18:40:39,509 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:39,574 [INFO] Building virtual cell stimulations for virt_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building virtual cell stimulations for virt_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:39,590 [INFO] Running simulation for 1000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Running simulation for 1000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:39,591 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:40:39,592 [INFO] Block save every 5000 steps\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Block save every 5000 steps\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:43:40,420 [INFO] step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,760 [INFO] step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,787 [INFO] Simulation completed in 5.0 minutes, 54.2 seconds \n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Simulation completed in 5.0 minutes, 54.2 seconds \n" + ] + } + ], + "source": [ + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.purkinje.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "642172f8-b7f3-4216-8a67-15e530b97c94", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 1.4. Example: Building your own python-based cell models " + ] + }, + { + "cell_type": "markdown", + "id": "ee20e24c-9767-44b8-9701-280526f7a379", + "metadata": {}, + "source": [ + "Another option is to build a cell instance yourself using the NEURON Python API. As with above, you just need to change the **model_template** parameter for the given cell(s) you want to build from scratch and create your own `@cell_model` function that will be called prior to the simulation to generate individual NEURON cell instances for appropriate node in the network. The code can be put inside your **run_bionet.py** script or imported from another file in the same directory.\n", + "\n", + "The following example is a simple model taken from the [NEURON ball-and-stick tutorial](https://neuron.yale.edu/neuron/docs/ball-and-stick-model-part-1) (See *build_network.ball_and_stick.py* file see how the network was created)." + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "9a309390-dbc4-4caf-8bfc-3a0a742d77f9", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "from bmtk.simulator.bionet.io_tools import io\n", + "from neuron import h\n", + "\n", + "class BallAndStick:\n", + " def __init__(self, cell):\n", + " self._gid = cell['node_id']\n", + " self._setup_morphology(cell)\n", + " self._setup_biophysics(cell)\n", + "\n", + " def _setup_morphology(self, cell):\n", + " self.soma = h.Section(name='soma', cell=self)\n", + " self.dend = h.Section(name='dend', cell=self)\n", + " self.all = [self.soma, self.dend]\n", + " self.dend.connect(self.soma)\n", + " self.soma.L = self.soma.diam = 12.6157\n", + " self.dend.L = 200\n", + " self.dend.diam = 1\n", + " \n", + " def _setup_biophysics(self, cell):\n", + " for sec in self.all:\n", + " sec.Ra = 100 # Axial resistance in Ohm * cm\n", + " sec.cm = 1 # Membrane capacitance in micro Farads / cm^2\n", + " self.soma.insert('hh') \n", + " for seg in self.soma:\n", + " seg.hh.gnabar = cell['gnabar'] # Sodium conductance in S/cm2\n", + " seg.hh.gkbar = cell['gkbar'] # Potassium conductance in S/cm2\n", + " seg.hh.gl = cell['gl'] # Leak conductance in S/cm2\n", + " seg.hh.el = cell['el'] # Reversal potential in mV\n", + " # Insert passive current in the dendrite # <-- NEW\n", + " self.dend.insert('pas') # <-- NEW\n", + " for seg in self.dend: # <-- NEW\n", + " seg.pas.g = cell['g_pas'] # Passive conductance in S/cm2 # <-- NEW\n", + " seg.pas.e = cell['e_pas'] # Leak reversal potential mV # <-- NEW \n", + " \n", + " def __repr__(self):\n", + " return 'BallAndStick[{}]'.format(self._gid)\n", + "\n", + "@bionet.cell_model(directive='python:loadBAS', model_type='biophysical')\n", + "def loadPurkinjeModel(cell, template_name, dynamics_params):\n", + " bas_cell = BallAndStick(cell)\n", + " io.log_info(f'Adding {bas_cell} to network')\n", + " return bas_cell\n" + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "id": "dd9efc30-b0cd-4f2c-8ace-d214f43a5e16", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,816 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-10-21 18:46:33,859 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "NEURON: Biophys : a template cannot be redefined\n", + " in Biophys.hoc near line 1\n", + " begintemplate Biophys\n", + " ^\n", + " xopen(\"Biophys.hoc\")\n", + " execute1(\"{xopen(\"Bi...\")\n", + " load_file(\"Biophys.hoc\")\n", + "NEURON: L5PCbiophys : a template cannot be redefined\n", + " in L5PCbiophys4.hoc near line 10\n", + " begintemplate L5PCbiophys\n", + " ^\n", + " xopen(\"L5PCbiophy...\")\n", + " execute1(\"{xopen(\"L5...\")\n", + " load_file(\"L5PCbiophy...\")\n", + "NEURON: L5PCtemplate : a template cannot be redefined\n", + " in L5PCtemplate.hoc near line 8\n", + " begintemplate L5PCtemplate\n", + " ^\n", + " xopen(\"L5PCtempla...\")\n", + " execute1(\"{xopen(\"L5...\")\n", + " load_file(\"L5PCtempla...\")\n", + "NEURON: HNTemplate : a template cannot be redefined\n", + " in HNTemplate.hoc near line 1\n", + " begintemplate HNTemplate\n", + " ^\n", + " xopen(\"HNTemplate.hoc\")\n", + " execute1(\"{xopen(\"HN...\")\n", + " load_file(\"HNTemplate.hoc\")\n", + "INFO:NEURONIOUtils:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,862 [INFO] Adding BallAndStick[0] to network\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Adding BallAndStick[0] to network\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,864 [INFO] Adding BallAndStick[1] to network\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Adding BallAndStick[1] to network\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,866 [INFO] Adding BallAndStick[2] to network\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Adding BallAndStick[2] to network\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,868 [INFO] Adding BallAndStick[3] to network\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Adding BallAndStick[3] to network\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,870 [INFO] Adding BallAndStick[4] to network\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Adding BallAndStick[4] to network\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,872 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,935 [INFO] Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,974 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,975 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:46:33,976 [INFO] Block save every 5000 steps\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Block save every 5000 steps\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:49:33,651 [INFO] step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:52:27,649 [INFO] step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:55:22,206 [INFO] step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:17,138 [INFO] step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:17,161 [INFO] Simulation completed in 11.0 minutes, 43.19 seconds \n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Simulation completed in 11.0 minutes, 43.19 seconds \n" + ] + } + ], + "source": [ + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.ball_and_stick.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "a3f5e4e4-5321-4bd0-a0ae-7f066940ceac", + "metadata": {}, + "source": [ + "For more information on how to build cells from scratch using NEURON see the [official documentation](https://www.neuron.yale.edu/neuron/static/py_doc/modelspec/programmatic/topology.html)." + ] + }, + { + "cell_type": "markdown", + "id": "3c8bec31-7364-4dce-b6eb-f2fd9691225e", + "metadata": {}, + "source": [ + "## 2. Modifying an existing cell model" + ] + }, + { + "cell_type": "markdown", + "id": "e467baa9-9dd9-4707-8b40-d2e20f6b7d50", + "metadata": {}, + "source": [ + "Very often, modelers may want to use an already existing cell model but make some minor changes of their own. For example, you may want to download a prebuilt channel mechanic (in the form of a .mod or .hoc file) to insert into a subset of an already existing network. You may want to edit the cell morphologies to add or remove sections of the neuron. You may want to adjust the channel densities based on the location of the section.\n", + "\n", + "One option to achieve this is to take an existing Hoc, Python, NeuroML, etc., cell model template and manually edit the file! This is **NOT** a good option for multiple reasons; your changes may lead to unintended side-effects - especially if you're not familiar with the language or how it was created. On top of that, having to rebuild the network and have multiple versions of a Hoc/Python template can be tedious if you are trying to test and debug your changes on the fly." + ] + }, + { + "cell_type": "markdown", + "id": "efd97775-98a0-41c3-b755-91912b4de19d", + "metadata": {}, + "source": [ + "**Solution**\n", + "\n", + "SONATA and BMTK provide support for making changes to an already instantiated cell model using the [**model_processing**](https://github.com/AllenInstitute/sonata/blob/master/docs/SONATA_DEVELOPER_GUIDE.md#nodes---optional-reserved-attributes) attribute. When an attribute is defined for a subpopulation of cells, it defines a directive and/or function to call on said cell immediately after it has been initialized.\n", + "\n", + "In BMTK, implementing your own custom function for \"model_processing\" is done in the same manner as with cell-loading. You can use the `@model_processing` decorator to a function that is inside or called in your *run_bionet.py* script.\n", + "\n", + "```python\n", + "from bmtk.simulator.bionent import model_processing\n", + "\n", + "@model_processing\n", + "def process_my_cell(hoc_obj, cell, dynamics_params):\n", + " updated_hoc_obj = # ... modify hoc object based on parameters stored in cell and/or dynamics params.\n", + " return updated_hoc_obj\n", + "```\n", + "\n", + "Then, when building the cells (or by editing either the nodes.h5 or node_types.csv file), you can add a reference to the `process_my_cell` function.\n", + "\n", + "```python\n", + "net.add_nodes(\n", + " model_type='biophysical',\n", + " model_template='hoc:Biophys1',\n", + " model_processing='process_my_cell`,\n", + " ...\n", + ")\n", + "```\n", + "\n", + "BMTK will also allow you to call multiple *model_process` functions in sequence by separating the calls by a comma:\n", + "\n", + "```python\n", + "net.add_nodes(\n", + " model_type='biophysical',\n", + " model_template='hoc:Biophys1',\n", + " model_processing='process_cell_1,process_cell_2`,\n", + " ...\n", + ")\n", + "```" + ] + }, + { + "cell_type": "markdown", + "id": "16035559-b787-464b-9dce-966715524956", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 2.1. Example: Adding customized mechanisms into an existing cell model " + ] + }, + { + "cell_type": "markdown", + "id": "577e0532-3a4e-4d11-9837-0828b5a65b27", + "metadata": {}, + "source": [ + "Besides cell models, repositories like ModelDB and Open Source Brain also contain a wide array of membrane density mechanisms (e.g., currents, and channels) that we may want to add to our own existing cell models. In NEURON, these are typically written as .mod files in the NMODL language and require pre-compilation using the *nrnivmodl* command. \n", + "\n", + "In this example, we will try downloading arbitrary sodium channel models and show how we can dynamically insert them into our L5 cells using the `@model_processing` directive." + ] + }, + { + "cell_type": "markdown", + "id": "988c638f-e0d1-466a-afe9-15e4fc1a3cc4", + "metadata": {}, + "source": [ + "#### Step 1: Downloading and compiling mechanisms\n", + "\n", + "First, we will find mechanisms models (e.g., .mod) files to use in our models. Here we [randomly choose sodium channels](https://modeldb.science/140249?tab=1) from ModelDB. Click on the download button to download the files.\n", + "\n", + "We would then need to move the downloaded .mod files into the *components/mechansism/modfiles* and rerun the command `cd components/mechanisms && nrnivmodl`. However, to make things more logical, BMTK allows us to have multiple *mechanisms* directories, so if it turns in the end, we don't want to use the new mechanisms, they will be easier to delete. Instead, we will put the .mod files into *components/dLGN_mechanisms/modfiles*, and run `nrnivmodl` from the new directory. Then, to make sure BMTK knows where to find the new mechanisms, we need to update the components section of the SONATA config:\n", + "\n", + "```json\n", + "\"components\": {\n", + " \"templates_dir\": \"$COMPONENTS_DIR/templates\",\n", + " \"mechanisms_dir\": [\n", + " \"$COMPONENTS_DIR/mechanisms\", \n", + " \"$COMPONENTS_DIR/dLGN_mechanisms\"\n", + " ],\n", + " ...\n", + "}\n", + "```" + ] + }, + { + "cell_type": "markdown", + "id": "2022b063-a091-4963-9860-a1ffaaf448bd", + "metadata": {}, + "source": [ + "#### Step 2: Setting directive for a population of neurons\n", + "\n", + "We need to make minor updates to our build network so that every subset of cells that will have these new mechanisms added will have model_processing attribute pointing to our customized post-initialization function.\n", + "\n", + "```python\n", + "net.add_nodes(\n", + " N=1,\n", + " model_type='biophysical',\n", + " model_template='hoc:L5PCtemplate',\n", + " morphology='cell3.asc',\n", + " model_processing='add_channels'\n", + ")\n", + "```\n", + "\n", + "Or if you don't want to rebuild the entire network, you can also just add a **model_processing** to the node_types.csv." + ] + }, + { + "cell_type": "markdown", + "id": "a68bc00a-207d-4c46-8372-c301d5d771d2", + "metadata": {}, + "source": [ + "#### Step 3: Add model_processing function and run\n", + "\n", + "Now, we can add our customized `add_channels` function, which will be called for each applicable cell right before the simulation. Like with the `@cell_model` directive, the function can be placed inside or imported into our *run_bionet.py* script, has a predefined signature, and should return an HOC instantiated cell object.\n", + "\n", + "Here, we will have our `add_channels` simply iterate through each section of the cell and insert the downloaded compiled IC channel:\n", + "```\n", + "@bionet.model_processing\n", + "def add_channels(hoc_obj, cell, dynamics_params):\n", + " for sec in hoc_obj.all:\n", + " if 'axon' in sec.name():\n", + " continue\n", + "\n", + " sec.insert('iahp')\n", + " setattr(sec, 'gkbar_iahp', 3.0e-04)\n", + " setattr(sec, 'taum_iahp', 0.5)\n", + " \n", + " return hoc_obj\n", + "```\n", + "Notes:\n", + "* For most models, we can use the `.all` property to iterate through every section of the cell. Depending on the model, you may also have section iterators like `.soma`, `.axon`, `.dend`, `.apical`, `.basal`.\n", + "* We don't want to insert IC channels into the axon sections. Unfortunately, there is no flag to indicate if a section is a soma, axon, dend, etc. So instead, we use the section's `name` to spike axons (Typically, a section name for a HOC or Python template will be **TemplateName\\[cell_id\\].axon\\[sec_num\\]**\n", + "* Next, we use the `.insert` command to insert the channel into our section\n", + "* We use the Python' setattr' function to change the default values of parameters. The specific mod file will determine which parameters can be changed and their range.\n", + "\n", + "You can run an example using:\n", + "```\n", + "$ python run.added_channels.py config.added_channels.json\n", + "```" + ] + }, + { + "cell_type": "markdown", + "id": "a3c29c73-4d20-4590-9a7b-f4068ef72906", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 2.2. Example: Modifying channel conductance in an instantiated cell. " + ] + }, + { + "cell_type": "markdown", + "id": "9e082778-9f86-4e8c-b9eb-1361868abd17", + "metadata": {}, + "source": [ + "Another thing we can also use the `@model_processing` directive is to update intrinsic properties, or even the morphology and topology, of a given cell right before running a simulation. \n", + "\n", + "In this example, we will want to take an already existing model and modify the conductance of the hyperpolarization-activated channels (**Ih**) in the apical dendrites based on their distance from the soma. After some investigation, we find that the conductance for our model is controlled by the parameter `gbar_Ih`. So, we want to half this parameter for all sections that are at a certain arc-length distance from the soma. We define `model_processing` function `adjust_densities`." + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "c2bd8ff4-0ed7-4cfc-aa97-453272362079", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "from bmtk.simulator.bionet.io_tools import io\n", + "\n", + "@bionet.model_processing\n", + "def adjust_densities(hoc_obj, cell, dynamics_params):\n", + " n_segs = 0\n", + " n_segs_updated = 0\n", + " for sec in hoc_obj.apic:\n", + " for seg in sec:\n", + " n_segs += 1\n", + " if h.distance(seg) > 1000:\n", + " n_segs_updated += 1\n", + " org_cond = getattr(seg, 'gbar_Ih')\n", + " new_cond = org_cond*0.5\n", + " setattr(seg, 'gbar_Ih', new_cond)\n", + " \n", + " io.log_info(f'{n_segs_updated} out of {n_segs} apical dendritic segments updated.')\n", + " return hoc_obj\n" + ] + }, + { + "cell_type": "markdown", + "id": "db0eb4a8-6759-456a-becf-bc46df2b4cc9", + "metadata": {}, + "source": [ + "Some notes to consider about this function:\n", + "* To only iterate through the **apic** section types (which will ignore soma, axon, and basal section), we use `hoc_obj.apic` list instead of `hoc_obj.all` as we did with the previous example\n", + "* A section may be partitioned into one or more segments. Each section will have the same attributes and inserted mechanisms. However, the actual property values of each individual segment, like distance and conductance, may vary. Hence, we need to update each individual segment of each section using the code:\n", + "```python\n", + " for section in hoc_obj.all:\n", + " for segment in section:\n", + " ....\n", + "```\n", + "* [`h.distance(seg)`](https://www.neuron.yale.edu/neuron/static/new_doc/modelspec/programmatic/topology/geometry.html#distance) is a built-in NEURON function that measures arc-length from each segment to the soma, in microns. Only segments that are >1000 um will have their Ih conductances reduced. The rest will be left to default value.\n", + "* We use the built-in Python functions `getattr` and `setattr` to fetch and set the gbar_Ih value of each segment object.\n" + ] + }, + { + "cell_type": "code", + "execution_count": 12, + "id": "4709c87c-9bf5-400d-990c-6bb87cdbec73", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:17,186 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-10-21 18:58:17,220 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:17,979 [INFO] 262 out of 573 apical dendritic segments updated.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:262 out of 573 apical dendritic segments updated.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:17,982 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:18,045 [INFO] Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:18,065 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Running simulation for 2000.000 ms with the time step 0.100 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:18,067 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Starting timestep: 0 at t_sim: 0.000 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 18:58:18,068 [INFO] Block save every 5000 steps\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Block save every 5000 steps\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:01:19,953 [INFO] step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:5000 t_sim:500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:04:13,908 [INFO] step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:10000 t_sim:1000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:07:06,708 [INFO] step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:15000 t_sim:1500.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:09:59,401 [INFO] step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils: step:20000 t_sim:2000.00 ms\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:09:59,432 [INFO] Simulation completed in 11.0 minutes, 41.37 seconds \n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Simulation completed in 11.0 minutes, 41.37 seconds \n" + ] + } + ], + "source": [ + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.modified_densities.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "d747b8e0-e8d0-49cf-9f99-21420d8e417c", + "metadata": {}, + "source": [ + "#### Help with investigating and updating a model\n", + "\n", + "Trying to figure out what mechanisms, section types, and other parameters of instantiated HOC cell can be tricky, as can trying to figure out how to change an existing cell. A good place to start is by looking through the official NEURON documentation [1](https://www.neuron.yale.edu/neuron/static/new_doc/modelspec/programmatic/topology/geometry.html) [2](https://www.neuron.yale.edu/neuron/static/new_doc/modelspec/programmatic/topology/geometry.html). One useful function is the `h.psection()` that will provide valuable information about each section/segment of a cell.\n", + "\n", + "Below, we can overwrite the previous version of `adjust_densities` with one that will print information about each section type (although only for the first section of each type - there are 1200+ sections in total, so we won't try to print them all out). Not only is this helpful for inspecting the cell, it shows how useful it can be to change the way cells are processed on the fly.\n" + ] + }, + { + "cell_type": "code", + "execution_count": 13, + "id": "5ee753b8-9267-4e0b-951c-dd7db5a785c5", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:09:59,446 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-10-21 19:09:59,464 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "L5PCtemplate[16].apic[0]\n", + "{'Ra': 100.0,\n", + " 'cell': L5PCtemplate[16],\n", + " 'cm': [2.0],\n", + " 'density_mechs': {'CaDynamics_E2': {'decay': [35.725651],\n", + " 'depth': [0.1],\n", + " 'gamma': [0.000637],\n", + " 'minCai': [0.0001]},\n", + " 'Ca_HVA': {'g': [0.0],\n", + " 'gbar': [1e-05],\n", + " 'h': [0.0],\n", + " 'ica': [0.0],\n", + " 'm': [0.0]},\n", + " 'Ca_LVAst': {'gCa_LVAst': [0.0],\n", + " 'gCa_LVAstbar': [0.00141954],\n", + " 'h': [0.0],\n", + " 'ica': [0.0],\n", + " 'm': [0.0]},\n", + " 'Ih': {'g': [0.0],\n", + " 'gbar': [0.0001309879],\n", + " 'ihcn': [0.0],\n", + " 'm': [0.0],\n", + " 'shift1': [154.9],\n", + " 'shift2': [11.9],\n", + " 'shift3': [0.0],\n", + " 'shift4': [33.1],\n", + " 'shift5': [6.43],\n", + " 'shift6': [193.0]},\n", + " 'Im': {'g': [0.0], 'gbar': [1e-05], 'ik': [0.0], 'm': [0.0]},\n", + " 'NaTs2_t': {'gNaTs2_t': [0.0],\n", + " 'gNaTs2_tbar': [0.021489],\n", + " 'h': [0.0],\n", + " 'ina': [0.0],\n", + " 'm': [0.0]},\n", + " 'SK_E2': {'gSK_E2': [0.0],\n", + " 'gSK_E2bar': [2e-06],\n", + " 'ik': [0.0],\n", + " 'z': [0.0]},\n", + " 'SKv3_1': {'gSKv3_1': [0.0],\n", + " 'gSKv3_1bar': [0.001808],\n", + " 'ik': [0.0],\n", + " 'm': [0.0]},\n", + " 'pas': {'e': [-90.0], 'g': [6e-05], 'i': [0.0]}},\n", + " 'hoc_internal_name': '__nrnsec_0x55555e538290',\n", + " 'ions': {'ca': {'cai': [5e-05],\n", + " 'cao': [2.0],\n", + " 'dica_dv_': [0.0],\n", + " 'eca': [132.4579341637009],\n", + " 'ica': [0.0]},\n", + " 'k': {'dik_dv_': [0.0],\n", + " 'ek': [-85.0],\n", + " 'ik': [0.0],\n", + " 'ki': [54.4],\n", + " 'ko': [2.5]},\n", + " 'na': {'dina_dv_': [0.0],\n", + " 'ena': [50.0],\n", + " 'ina': [0.0],\n", + " 'nai': [10.0],\n", + " 'nao': [140.0]}},\n", + " 'morphology': {'L': 16.729761891618992,\n", + " 'diam': [8.118265357665159],\n", + " 'parent': L5PCtemplate[16].soma[0](0.5),\n", + " 'pts3d': [(-39.77000045776367,\n", + " 29.8799991607666,\n", + " -24.969999313354492,\n", + " 9.550000190734863),\n", + " (-39.77000045776367,\n", + " 29.8799991607666,\n", + " -24.969999313354492,\n", + " 9.550000190734863),\n", + " (-39.54999923706055,\n", + " 33.040000915527344,\n", + " -26.06999969482422,\n", + " 8.640000343322754),\n", + " (-38.86000061035156,\n", + " 37.06999969482422,\n", + " -27.100000381469727,\n", + " 7.730000019073486),\n", + " (-37.95000076293945,\n", + " 39.939998626708984,\n", + " -27.56999969482422,\n", + " 7.730000019073486),\n", + " (-37.5,\n", + " 42.2400016784668,\n", + " -27.56999969482422,\n", + " 7.730000019073486),\n", + " (-37.04999923706055,\n", + " 45.689998626708984,\n", + " -29.020000457763672,\n", + " 7.730000019073486)],\n", + " 'trueparent': L5PCtemplate[16].soma[0](0.5)},\n", + " 'name': 'L5PCtemplate[16].apic[0]',\n", + " 'nseg': 1,\n", + " 'point_processes': {},\n", + " 'regions': set(),\n", + " 'species': set()}\n", + "2024-10-21 19:10:00,357 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 19:10:00,363 [INFO] Building virtual cell stimulations for virt_exc_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:NEURONIOUtils:Building virtual cell stimulations for virt_exc_spikes\n" + ] + } + ], + "source": [ + "from pprint import pprint\n", + "\n", + "@bionet.model_processing(name='adjust_densities')\n", + "def check_mechanisms(hoc_obj, cell, dynamics_params):\n", + " for sec in hoc_obj.apic:\n", + " print(sec.name())\n", + " pprint(sec.psection())\n", + " break\n", + "\n", + " return hoc_obj\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.modified_densities.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "d1dd8d48-ad1a-4790-9f19-040f053cb430", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3 (ipykernel)", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.9.7" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/01_opt_external_models/Hay_Model_SingleCell.ipynb b/docs/tutorial/01_opt_external_models/Hay_Model_SingleCell.ipynb new file mode 100644 index 00000000..369a5803 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/Hay_Model_SingleCell.ipynb @@ -0,0 +1,288 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "9e6ef3db", + "metadata": {}, + "source": [ + "# Step 1. Build the cell" + ] + }, + { + "cell_type": "markdown", + "id": "abe9d87f", + "metadata": {}, + "source": [ + "Here we just have to specify the name of the hoc-template and the morphology, files which should be found in `components/templates/` and `components/morphologies`, respectively.\n", + "\n", + "In this model, the dynamic params are built into the .hoc files so there is no need to specify a **dynamics_params** attribute. Similarly there is no need for axon post-processing in these models so we no longer have a **model_processing** attribute.\n", + "\n", + "The other attributes are optional and can be removed or used for the Hay model specifically." + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "cf9f4baa", + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "WARNING:root:No edges have been made for this network, skipping saving of edges file.\n" + ] + } + ], + "source": [ + "from bmtk.builder.networks import NetworkBuilder\n", + "\n", + "net = NetworkBuilder('cell')\n", + "net.add_nodes(\n", + " N=1,\n", + " model_type='biophysical',\n", + " model_template='hoc:L5PCtemplate.hoc',\n", + " morphology='cell3.asc',\n", + " \n", + " # optional\n", + " # ei_type='exc',\n", + " # model_name='Scnn1a',\n", + " # species='mouse',\n", + " # location='VISp',\n", + " # layer='L4'\n", + ")\n", + "net.build()\n", + "net.save(output_dir='network')" + ] + }, + { + "cell_type": "markdown", + "id": "d4887333", + "metadata": {}, + "source": [ + "\n", + "Next, we can add synaptic inputs like we did in the workshop. Note that some properties like **target_sections** and **syn_weight** can and should be adjusted according to the target model type." + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "38cdd4b2", + "metadata": {}, + "outputs": [], + "source": [ + "virt_exc = NetworkBuilder('virt_exc')\n", + "virt_exc.add_nodes(\n", + " N=20,\n", + " model_type='virtual',\n", + " ei_type='exc'\n", + ")\n", + "conns = virt_exc.add_edges(\n", + " source=virt_exc.nodes(),\n", + " target=net.nodes(),\n", + " connection_rule=12,\n", + " model_template='Exp2Syn',\n", + " dynamics_params='AMPA_ExcToExc.json',\n", + " distance_range=[0.0, 1.0e20],\n", + " target_sections=['soma', 'basal', 'apical'],\n", + " delay=2.0,\n", + " syn_weight=0.01\n", + ")\n", + "\n", + "virt_exc.build()\n", + "virt_exc.save(output_dir='network')" + ] + }, + { + "cell_type": "markdown", + "id": "d3230a4d", + "metadata": {}, + "source": [ + "# Step 2: Running the model" + ] + }, + { + "cell_type": "markdown", + "id": "06ba21d8", + "metadata": {}, + "source": [ + "To create a cell object from the L5PCtemplate.hoc, we need some specialized code to load in the NEURON template. To do that, we create a special function called loadHayModel that includes customized Python instructions for initializing and building our cell. \n", + "\n", + "We use the custom Python **decorator** `@bionet.cell_model` to tell BMTK that whenever a cell has **model_template=hoc:L5PCtemplate.hoc\"**, it should call the `loadHayModel` function, which will build the cell and return it as a HOC object. We can also use the `cell_model` template for loading other models, if necessary." + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "a8f05216", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "from neuron import h\n", + "\n", + "\n", + "h.load_file(\"import3d.hoc\")\n", + "\n", + "@bionet.cell_model(directive='hoc:L5PCtemplate.hoc', model_type='biophysical')\n", + "def loadHayModel(cell, template_name, dynamics_params):\n", + " morphology_file = cell['morphology']\n", + " hobj = h.L5PCtemplate(str(morphology_file))\n", + " return hobj" + ] + }, + { + "cell_type": "markdown", + "id": "8842ff7c", + "metadata": {}, + "source": [ + "After that the simulation can be ran like normal" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "a751669e", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-04-29 11:29:02,857 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "NEURON: Import3d_Section : a template cannot be redefined\n", + " in import3d/import3d_sec.hoc near line 1\n", + " begintemplate Import3d_Section\n", + " ^\n", + " xopen(\"import3d/i...\")\n", + " xopen(\"import3d.hoc\")\n", + " execute1(\"{xopen(\"im...\")\n", + " load_file(\"/local1/wo...\")\n", + "NEURON: Im is not a MECHANISM\n", + " in L5PCbiophys4.hoc near line 22\n", + " \tinsert Im \n", + " ^\n", + " xopen(\"L5PCbiophy...\")\n", + " " + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "NEURON mechanisms not found in ./components/mechanisms.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + " execute1(\"{xopen(\"L5...\")\n", + " load_file(\"L5PCbiophy...\")\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-04-29 11:29:03,148 [INFO] Building cells.\n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "# conf = bionet.Config.from_json('config.iclamp.json')\n", + "conf = bionet.Config.from_json('config.with_syns.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "1c4fef7b", + "metadata": {}, + "source": [ + "Like with the Allen Institute model, the Hay NEURON model will record variables like spikes, membrane traces, and other parameters that BMTK will instruct it to. The output is in the same format, and thus, we can read it like with the models we used in the workshop." + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "fc6b31b7", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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\n", 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.spike_trains import plot_raster\n", + "from bmtk.analyzer.compartment import plot_traces\n", + "\n", + "_ = plot_raster(config_file='config.with_syns.json')\n", + "_ = plot_traces(config_file='config.with_syns.json', report_name='v_report')" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "6f7fa777", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3 (ipykernel)", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.12.3" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/01_opt_external_models/Purkinje_morpho_1.py b/docs/tutorial/01_opt_external_models/Purkinje_morpho_1.py new file mode 100644 index 00000000..f20e5c87 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/Purkinje_morpho_1.py @@ -0,0 +1,1054 @@ +from neuron import h +import numpy as np +import random +import math +import sys +from random import choice + +from Purkinje_morpho_1_number import number_ind_1 + +from Synapses import Synapse_py3 + +class Purkinje_Morpho_1: + def __init__(self,spines_on = 0): + + h.load_file('stdlib.hoc') + h.load_file('import3d.hoc') + + cell = h.Import3d_Neurolucida3() + cell.input('morphology/soma_10c.asc') + + + i3d = h.Import3d_GUI(cell,0) + i3d.instantiate(self) + + #the file contains all the results for the population. Some are good, some are bad, some are ugly. + conductvalues = np.genfromtxt("R_01_final_pop.txt") + indiv_number = number_ind_1['indiv'] + print( 'indiv number', indiv_number) + + + self.x = self.y = self.z = 0 + def set_position(x, y, z): + for sec in self.all: + for i in range(sec.n3d()): + sec.pt3dchange(i, + x - self.x + sec.x3d(i), + y - self.y + sec.y3d(i), + z - self.z + sec.z3d(i), + sec.diam3d(i)) + self.x, self.y, self.z = x, y, z + + self.soma[0].nseg = 1 + (2 * int(self.soma[0].L / 40)) + self.soma[0].cm = 2 + self.soma[0].Ra = 122 + + self.soma[0].insert('Leak') + self.soma[0].e_Leak = -61 + self.soma[0].gmax_Leak = 1E-3 + + self.soma[0].insert('Nav1_6') + self.soma[0].gbar_Nav1_6 = conductvalues[indiv_number, 14] + self.soma[0].ena = 60 + + self.soma[0].insert('Kv1_1') + self.soma[0].gbar_Kv1_1 = conductvalues[indiv_number, 15] + + self.soma[0].insert('Kv1_5') + self.soma[0].gKur_Kv1_5 = conductvalues[indiv_number, 35] + + self.soma[0].insert('Kv3_4') + self.soma[0].gkbar_Kv3_4 = conductvalues[indiv_number, 16] + + self.soma[0].insert('Kir2_3') + self.soma[0].gkbar_Kir2_3 = conductvalues[indiv_number, 17] + + self.soma[0].insert('Cav2_1') + self.soma[0].pcabar_Cav2_1 = conductvalues[indiv_number,18] + + self.soma[0].insert('Cav3_1') + self.soma[0].pcabar_Cav3_1 = conductvalues[indiv_number,19] + + self.soma[0].insert('Cav3_2') + self.soma[0].gcabar_Cav3_2 =conductvalues[indiv_number,20] + + self.soma[0].insert('Cav3_3') + self.soma[0].pcabar_Cav3_3 = conductvalues[indiv_number,21] + + self.soma[0].insert('Kca1_1') + self.soma[0].gbar_Kca1_1 = conductvalues[indiv_number,22] + + self.soma[0].insert('Kca2_2') + self.soma[0].gkbar_Kca2_2 = conductvalues[indiv_number,23] + + self.soma[0].insert('Kca3_1') + self.soma[0].gkbar_Kca3_1 = conductvalues[indiv_number,24] + self.soma[0].ek = -88 + + self.soma[0].insert('HCN1') + self.soma[0].gbar_HCN1 = conductvalues[indiv_number,25] + self.soma[0].eh = -34.4 + + self.soma[0].insert('cdp5_CAM') + + self.soma[0].TotalPump_cdp5_CAM = 5e-8 + + self.soma[0].push() + self.soma[0].eca = 137.52625 + + h.define_shape() + if spines_on == 1: #only for spines + set_position(200, 962, 0) + h.pop_section() + + self.whatami = "prk" + +#Dend coordinate + + + for d in self.dend: + d.Ra = 122 + if spines_on == 1: + d.cm = 2 + if spines_on == 0: + d.cm = (11.510294 * math.exp( - 1.376463 * d.diam) + 2.120503) + + d.nseg = 1 + (2 * int(d.L / 40)) + + d.insert('Leak') + d.e_Leak = -61 + d.gmax_Leak = 0.0003 + + d.insert('Kv3_3') + d.gbar_Kv3_3 = conductvalues[indiv_number,3] + + d.insert('Kv4_3') + d.gkbar_Kv4_3 = conductvalues[indiv_number,4] + + d.insert('Cav2_1') + d.pcabar_Cav2_1 = conductvalues[indiv_number,6]*6 + + d.insert('Cav3_3') + d.pcabar_Cav3_3 = conductvalues[indiv_number,9] + + d.insert('Kca1_1') + d.gbar_Kca1_1 = conductvalues[indiv_number,10] + + d.insert('HCN1') + d.gbar_HCN1 = conductvalues[indiv_number,13] + d.eh = -34.4 + + d.insert('Kca2_2') + d.gkbar_Kca2_2 = conductvalues[indiv_number,11] + + d.insert('cdp5_CAM') + + d.TotalPump_cdp5_CAM = 6e-8 + + if d.diam >= 1.6: + d.cm = 2 + + d.insert('Kv1_1') + d.gbar_Kv1_1 = conductvalues[indiv_number,1] + + d.insert('Kv1_5') + d.gKur_Kv1_5 = conductvalues[indiv_number,2] + + d.insert('Kir2_3') + d.gkbar_Kir2_3 = conductvalues[indiv_number,5] + + d.insert('Cav3_1') + d.pcabar_Cav3_1 = conductvalues[indiv_number,7] + + d.insert('Cav3_2') + d.gcabar_Cav3_2 = conductvalues[indiv_number,8] + + d.insert('Kca3_1') + d.gkbar_Kca3_1 = conductvalues[indiv_number,12] + + if d.diam >=3.3: + d.cm = 2 + d.insert('Nav1_6') + d.gbar_Nav1_6 = conductvalues[indiv_number,0] + d.ena = 60 + + d.ek = -88 + + d.push() + d.eca = 137.52625 + h.pop_section() + + + +#Axon AIS. First section after the soma[0] + self.axonAIS = h.Section(name='axonAIS') + self.axonAIS.L = 17 #from Foust 2010 and clark 2005 and Somogyi76 + self.axonAIS.nseg = 1 + self.axonAIS.diam = 0.97 #ito 1983 + self.axonAIS.cm = 1 + + self.axonAIS.Ra = 122 + + self.axonAIS.insert('Leak') + self.axonAIS.e_Leak = -61 + self.axonAIS.gmax_Leak = 0.0003 + + self.axonAIS.insert('Nav1_6') + self.axonAIS.gbar_Nav1_6 = conductvalues[indiv_number,26]*1.5 + self.axonAIS.ena = 60 + + self.axonAIS.insert('Kv3_4') + self.axonAIS.gkbar_Kv3_4 = conductvalues[indiv_number,27] + self.axonAIS.ek = -88 + + self.axonAIS.insert('Cav2_1') + self.axonAIS.pcabar_Cav2_1 = conductvalues[indiv_number,28] + + self.axonAIS.insert('Cav3_1') + self.axonAIS.pcabar_Cav3_1 = conductvalues[indiv_number,29] + + self.axonAIS.insert('cdp5_CAM') + + self.axonAIS.TotalPump_cdp5_CAM = 2e-8 + + self.axonAIS.push() + self.axonAIS.eca = 137.52625 + h.pop_section() + +#AISK + + self.axonAISK = h.Section(name='axonAISK') + self.axonAISK.L = 4 + self.axonAISK.nseg = 1 + self.axonAISK.diam = 0.97 + self.axonAISK.cm = 1 + + self.axonAISK.Ra = 122 + + self.axonAISK.insert('Leak') + self.axonAISK.e_Leak = -61 + self.axonAISK.gmax_Leak = 0.0003 + + self.axonAISK.insert('Kv1_1') + self.axonAISK.gbar_Kv1_1 = conductvalues[indiv_number,30] + self.axonAISK.ek = -88 #aggiunto 28 gennaio 2016. + + + +#First Myelination + + self.axonmyelin = h.Section(name='axonmyelin') + self.axonmyelin.L = 100 #da 60 a 150um + self.axonmyelin.nseg = 1 + (2 * int(self.axonmyelin.L / 40)) + self.axonmyelin.diam = 0.73 + + + self.axonmyelin.insert('pas') #from brill77 + self.axonmyelin.e_pas = -61 #from brill77 + self.axonmyelin.g_pas = 5.60e-9 #from brill77 + self.axonmyelin.cm = 1.87e-11 #from brill77 + self.axonmyelin.Ra = 122 + +#NEW NODES of RANVIER + + secnumber = 3 + +#The IN paranode + self.renv_node = [h.Section(name='renv_node'+str(x)) for x in range(secnumber)] + + for a in self.renv_node: + a.L = 4 + a.nseg = 1 #+ (2 * int(a.L / 40)) + a.diam = 0.73 + a.cm = 1 + + a.Ra = 122 + + a.insert('Leak') + a.e_Leak = -61 + a.gmax_Leak = 0.0003 + + a.insert('Nav1_6') + a.gbar_Nav1_6 = conductvalues[indiv_number,31] #0.03 + a.ena = 60 + + a.insert('Kv3_4') + a.gkbar_Kv3_4 = conductvalues[indiv_number,32] #0.02 + a.ek = -88 + + a.insert('Cav2_1') + a.pcabar_Cav2_1 = conductvalues[indiv_number,33] #1e-5 + + a.insert('Cav3_1') + a.pcabar_Cav3_1 = conductvalues[indiv_number,34] #2.2e-4 + + a.insert('cdp5_CAM') + + a.TotalPump_cdp5_CAM = 5e-7 + + a.push() + a.eca = 137.52625 + h.pop_section() + +#Myelin sections + self.axoninode = [h.Section(name='axoninode'+str(x)) for x in range(secnumber)] + for d in self.axoninode: + d.L = 100 + d.nseg = 1 #+ (2 * int(d.L / 40)) + d.diam = 0.73 + + + d.insert('pas') #from brill77 + d.e_pas = -61 #from brill77 + d.g_pas = 5.60e-9 #from brill77 + d.cm = 1.87e-11 #from brill77 + d.Ra = 122 + + +#Collateral. + self.axoncoll = h.Section(name='axoncoll') + self.axoncoll.nseg = 1 + self.axoncoll.diam = 0.6 + self.axoncoll.L = 100 + #self.axoncoll.Ra = 122 #critical + + self.axoncoll.insert('Leak') + self.axoncoll.e_Leak = -61 + self.axoncoll.gmax_Leak = 0.0003 + + self.axoncoll.insert('Nav1_6') + self.axoncoll.gbar_Nav1_6 = 0.03 + self.axoncoll.ena = 60 + + self.axoncoll.insert('Kv3_4') + self.axoncoll.gkbar_Kv3_4 = 0.02 + self.axoncoll.ek = -88 + + self.axoncoll.insert('Cav3_1') + self.axoncoll.pcabar_Cav3_1 = 1e-5 + + self.axoncoll.insert('Cav2_1') + self.axoncoll.pcabar_Cav2_1 = 2.2e-4 + + self.axoncoll.insert('cdp5_CAM') + + + self.axoncoll.TotalPump_cdp5_CAM = 5e-7 + + self.axoncoll.push() + h.ion_style("ca_ion", 1, 1, 0, 1, 0) + self.axoncoll.eca = 137.52625 + self.axoncoll.cai = h.cai0_ca_ion + self.axoncoll.cao = h.cao0_ca_ion + h.pop_section() + +#Collateral second part + self.axoncoll2 = h.Section(name='axoncoll2') + self.axoncoll2.nseg = 1 + self.axoncoll2.diam = 0.6 + self.axoncoll2.L = 100 + #self.axoncoll2.Ra = 122 + + self.axoncoll2.insert('Leak') + self.axoncoll2.e_Leak = -61 + self.axoncoll2.gmax_Leak = 0.0003 + + self.axoncoll2.insert('Nav1_6') + self.axoncoll2.gbar_Nav1_6 = 0.03 + self.axoncoll2.ena = 60 + + + self.axoncoll2.insert('Kv3_4') + self.axoncoll2.gkbar_Kv3_4 = 0.02 + self.axoncoll2.ek = -88 + + self.axoncoll2.insert('cdp5_CAM') + + self.axoncoll2.insert('Cav3_1') + self.axoncoll2.pcabar_Cav3_1 = 1e-5 + + self.axoncoll2.insert('Cav2_1') + self.axoncoll2.pcabar_Cav2_1 = 2.2e-4 + + self.axoncoll2.TotalPump_cdp5_CAM = 5e-7 + + self.axoncoll2.push() + h.ion_style("ca_ion", 1, 1, 0, 1, 0) + self.axoncoll2.eca = 137.52625 + self.axoncoll2.cai = h.cai0_ca_ion + self.axoncoll2.cao = h.cao0_ca_ion + h.pop_section() + + self.axonAIS.connect(self.soma[0],0,0) + self.axonAISK.connect(self.axonAIS,1,0) + self.axonmyelin.connect(self.axonAISK,1,0) #myelin1 + self.renv_node[0].connect(self.axonmyelin,1,0) #node1 + self.axoninode[0].connect(self.renv_node[0],1,0) #myelin2 + self.renv_node[1].connect(self.axoninode[0],1,0) #node2 + self.axoninode[1].connect(self.renv_node[1],1,0) #myelin3 + self.renv_node[2].connect(self.axoninode[1],1,0) #node3 + self.axoninode[2].connect(self.renv_node[2],1,0) #myelin4 + + self.axoncoll.connect(self.renv_node[1],1,0) + self.axoncoll2.connect(self.axoncoll,1,0) + + if spines_on == 1: + dend_index = [] + dend_local_len = [] + dend_len = [] + spine_location = [] + self.dend_spine = [] + + #number of spikes per micron + spine_per_micron = 2 + + for e in self.dend: + if e.diam >= 0 and e.diam < 1.5: + self.dend_spine.append(e) + + original_dend_index = [] + for old_index, section_acess in enumerate(self.dend): + if section_acess.diam >= 0 and section_acess.diam < 1.5: + original_dend_index.append(old_index) + + + for local_index, x_dend in enumerate(self.dend_spine): + dend_index.append(local_index) + dend_local_len.append(int(x_dend.L) * spine_per_micron) + + dend_len.append(int(x_dend.L) * spine_per_micron) + + to_list = np.linspace(0.05, 0.95, num = int(x_dend.L) * spine_per_micron) + spine_location.append(to_list.tolist()) + + dend_total_len = sum(dend_len) + info_dend = list(zip(dend_index,dend_local_len)) + + info_dend_old_index = list(zip(original_dend_index,dend_local_len)) + spine_number = int(dend_total_len) + somma_location_spines = sum(spine_location,[]) + + + self.spine_head = [h.Section(name='spine_head_'+str(x)) for x in range(spine_number)] + + for i in self.spine_head: + i.nseg = 1 + i.diam = 1 + i.cm = 2 + i.L = 0.35 + i.Ra = 122 + + i.insert('Leak') + i.e_Leak = -61 + i.gmax_Leak = 1e-5 + + i.insert('Cav2_1') + i.pcabar_Cav2_1 = 1e-5 + + i.insert('Cav3_1') + i.pcabar_Cav3_1 = 1e-6 + + i.insert('Kca1_1') + i.gbar_Kca1_1 = 0.01 + + i.insert('Kca2_2') + i.gkbar_Kca2_2 = 1e-3 + + i.insert('Kv4_3') + i.gkbar_Kv4_3 = 0.005 + + + + i.insert('cdp5_CAM') + i.Nannuli_cdp5_CAM = 0.326 + (1.94 * (i.diam)) + (0.289*(i.diam)*(i.diam)) - ((3.33e-2)*(i.diam)*(i.diam)*(i.diam)) + ((1.55e-3)*(i.diam)*(i.diam)*(i.diam)*(i.diam)) - (2.55e-5*(i.diam)*(i.diam)*(i.diam)*(i.diam)*(i.diam)) + i.Buffnull2_cdp5_CAM = 64.2 - 57.3* math.exp(-(i.diam)/1.4) + i.rf3_cdp5_CAM = 0.162 - 0.106* math.exp(-(i.diam)/2.29) + i.rf4_cdp5_CAM = 0.003 + + i.push() + h.ion_style("ca_ion", 1, 1, 0, 1, 0) + i.eca = 137.52625 + i.cai = h.cai0_ca_ion + i.cao = h.cao0_ca_ion + h.pop_section() + + i.TotalPump_cdp5_CAM = 7e-10 + +#NECK OF THE SPINE + self.spine_neck = [h.Section(name='spine_neck_'+str(x)) for x in range(spine_number)] + + for x in self.spine_neck: + x.nseg = 1 + x.diam = 0.2 + x.cm = 3 + x.L = 0.7 + x.Ra = 122 + + x.insert('Leak') + x.e_Leak = -61 + x.gmax_Leak = 1e-5 + + + for i,j in enumerate(self.spine_head): + j.connect(self.spine_neck[i],1,0) + + print("spine active!!") + + list_dend_local = [] + c,v = zip(*info_dend) + for i in range(len(info_dend)): + new_list = [] + new_list = [c[i]]*v[i] + + + list_dend_local.append(new_list) + + list_dend_old_index = [] + c_old,v_old = zip(*info_dend_old_index) + for i in range(len(info_dend_old_index)): + new_list_oldindex = [] + + new_list_oldindex = [c_old[i]]*v_old[i] + + + list_dend_old_index.append(new_list_oldindex) + + list_dend_complete = sum(list_dend_local,[]) + + list_dend_complete_oldindex = sum(list_dend_old_index,[]) + + + self.total_list = list(zip(range(dend_total_len), list_dend_complete, somma_location_spines)) + + + print('dend_total_len', len(range(dend_total_len))) + print('list_dend_complete', len(list_dend_complete)) + print('somma_location_spines', len(somma_location_spines)) + + spine_sec_aa = [] + spine_sec_pf = [] + spine_sec_aa_SC = [] + + spine_print_list = range(0, len(self.total_list), 500) + + + c_source,v_dest,z_step = zip(*self.total_list) # outside the for... not inside.... + for spines_created in range(len(self.total_list)): + if spines_created in spine_print_list: + print('spines_placed', spines_created) + #AA + if self.dend_spine[v_dest[spines_created]].diam <= 0.3: + self.spine_neck[c_source[spines_created]].connect(self.dend_spine[v_dest[spines_created]],z_step[spines_created],0) + spine_sec_aa.append(c_source[spines_created]) + + #AA/SC + if self.dend_spine[v_dest[spines_created]].diam > 0.3 and self.dend_spine[v_dest[spines_created]].diam <= 0.75: + self.spine_neck[c_source[spines_created]].connect(self.dend_spine[v_dest[spines_created]],z_step[spines_created],0) + spine_sec_aa_SC.append(c_source[spines_created]) + + #PF/SC + if self.dend_spine[v_dest[spines_created]].diam > 0.75 and self.dend_spine[v_dest[spines_created]].diam <= 1.5: + self.spine_neck[c_source[spines_created]].connect(self.dend_spine[v_dest[spines_created]],z_step[spines_created],0) + spine_sec_pf.append(c_source[spines_created]) + + #AA + self.spine_head_aa = [] + #SC + self.spine_head_aa_SC = [] + #PF + self.spine_head_pf = [] + + spine_print_list_syn = range(0, len(self.spine_head), 5000) + + for i_head, e_head in enumerate(self.spine_head): + if i_head in spine_print_list_syn: + print('spines_syn: ', e_head) + elif i_head in spine_sec_aa: + self.spine_head_aa.append(e_head) + elif i_head in spine_sec_aa_SC: + self.spine_head_aa_SC.append(e_head) + elif i_head in spine_sec_pf: + self.spine_head_pf.append(e_head) + elif i_head in spine_sec_CF: + self.spine_head_CF.append(e_head) + + + + print('spine_head_aa between 0 - 0.3 ->', len(self.spine_head_aa)) + print('spine_head_aa_SC between 0.3 - 0.75 ->', len(self.spine_head_aa_SC)) + print('spine_head_pf_SC between 0.75 - 1.5 ->', len(self.spine_head_pf)) + + print('!!!!!!!!!!!!!!! spine_total', len(self.spine_head_aa_SC) + len(self.spine_head_aa) + len(self.spine_head_pf), ' !!!!!!!!!!!!!!!') + + + self.dendAA_SC = [] + self.dendAA_SC_index = [] + + self.dendPF_SC = [] + self.dendPF_SC_index = [] + + for i,e in enumerate(self.dend): + if e.diam > 0.3 and e.diam < 0.75: + self.dendAA_SC.append(e) + self.dendAA_SC_index.append(i) + + if e.diam > 0.75 and e.diam < 1.5: + self.dendPF_SC.append(e) + self.dendPF_SC_index.append(i) + + +#Recorder section. + + + self.time_vector = h.Vector() + self.time_vector.record(h._ref_t) + + self.vm_soma = h.Vector() + self.vm_soma.record(self.soma[0](0.5)._ref_v) + + + def createsyn(self, npf, naa, numpfstl): + + + + self.dendparallel = [] + self.dendascending = [] + + self.dendAAstl = [] + self.dendPFstl = [] + + #new subdivision for the synapses + for i in self.dend: + if i.diam > 0.75 and i.diam < 1.5: + self.dendparallel.append(i) + self.dendPFstl.append(i) + + if i.diam <= 0.75: + self.dendascending.append(i) + + + + print("PF") + print(len(self.dendparallel)) + print("AA") + print(len(self.dendascending)) + + #Parallel fiber list + self.PF_L = [] + + #Ascending axon list + self.AA_L = [] + + #Stellate list + self.SC_L = [] + + #Randomizer + self.pfrand = [] + self.aarand = [] + self.stlrand = [] + self.bgrand = [] + + + self.pfstlrand = [] + self.aastlrand = [] + + #New lists for the new stellate setup + + self.pfSC_L = [] + self.aaSC_L = [] + self.cfSC_L = [] + + + + n = len(self.dendparallel) + n2 = len(self.dendascending) + npfstl = len(self.dendPFstl) + + + +#PF random + for j in range(npf): + n -= 1 + i = random.randint(0, n) + self.dendparallel[i], self.dendparallel[n] = self.dendparallel[n], self.dendparallel[i] + self.pfrand.append(self.dendparallel[n]) + self.PF_L.append(Synapse_py3('pf',self,self.pfrand[j])) + + +#AA random + for j in range(naa): + n2 -= 1 + i = random.randint(0, n2) + self.dendascending[i], self.dendascending[n2] = self.dendascending[n2], self.dendascending[i] + self.aarand.append(self.dendascending[n2]) + self.AA_L.append(Synapse_py3('aa',self,self.aarand[j])) + +#STl_PF random + for j in range(numpfstl): + npfstl -= 1 + i = random.randint(0, npfstl) + self.dendPFstl[i], self.dendPFstl[npfstl] = self.dendPFstl[npfstl], self.dendPFstl[i] + self.stlrand.append(self.dendPFstl[npfstl]) + self.SC_L.append(Synapse_py3('stl',self,self.stlrand[j])) + +#revised code 2020 python3 + def dendrites_xy_nospine(self,min_x, max_x, min_y,max_y,syntype):#,option_random,number_random_syn): + + self.dend_cood_x = {} + self.dend_cood_y = {} + for i, j in enumerate(self.dend): + j.push() + self.dend_cood_x["x0_"+str(i)] = h.x3d(0) + self.dend_cood_y["y0_"+str(i)] = h.y3d(0) + h.pop_section() + + #only of X + dend_x_values = sorted(self.dend_cood_x.values()) #sorting of the values + dend_x_kyes = sorted(self.dend_cood_x, key=self.dend_cood_x.get) #sorting of the keys based on the values + dend_list_zip_x = zip(dend_x_kyes, dend_x_values) + + #print 'dimensions X' + #print min_x + #print max_x + + #only of Y + dend_y_values = sorted(self.dend_cood_y.values()) #sorting of the values + dend_y_kyes = sorted(self.dend_cood_y, key=self.dend_cood_y.get) #sorting of the keys based on the values + dend_list_zip_y = zip(dend_y_kyes, dend_y_values) + + #print 'dimensions Y' + #print min_y + #print max_y + + self.x_coord_dend = [] + xlist = [] + for i in dend_list_zip_x: + if i[1] > min_x and i[1] < max_x: + xlist.append(i[1]) + self.x_coord_dend.append(int(i[0][3:])) + + + self.y_coord_dend = [] + ylist = [] + for i in dend_list_zip_y: + if i[1] > min_y and i[1] < max_y: + ylist.append(i[1]) + self.y_coord_dend.append(int(i[0][3:])) + + #print "dend number" + #print len(self.x_coord_dend) + #print len(self.y_coord_dend) + + self.total_dend = [] + self.total_dend = set(self.x_coord_dend).intersection(self.y_coord_dend) + + #print 'number syn' + #print self.total_dend + + print('dend set', self.total_dend) + print('Dend synapses ON.') + + self.diam = [] + for x in self.total_dend: + self.diam.append(self.dend[x].diam) + + self.dend_diam = zip(self.total_dend, self.diam) + + #new list of synapses + + self.AAtotal = [] + self.PFtotal = [] + self.SCtotal = [] + + if syntype == 0: + self.AAdendminmax = [] + if syntype == 1: + self.PFdendminmax = [] + if syntype == 2: + self.SCdendminmax = [] + + for x in self.dend_diam: + if syntype == 0: + if x[1] <= 0.75: + #if option_random == 1: + #self.AAtotal.append(self.dend[x[0]]) + #else: + self.AAdendminmax.append(Synapse_py3('aa',self,self.dend[x[0]])) + #print('AA') + + if syntype == 1: + if x[1] > 0.75 and x[1] < 1.6: + #if option_random == 1: + #self.PFtotal.append(self.dend[x[0]]) + ##print('x[0]', x[0]) + #else: + self.PFdendminmax.append(Synapse_py3('pf',self,self.dend[x[0]])) + #print('x[0]', x[0]) + #print('nope') + + if syntype == 2: + if x[1] >= 0.3 and x[1] < 1.6: + #if option_random == 1: + #self.SCtotal.append(self.dend[x[0]]) + #else: + self.SCdendminmax.append(Synapse_py3('stl',self,self.dend[x[0]])) + + if syntype == 0: + print('AA syn number!!!!!!!!!!!!!!!!!!!!!!!!!!!', len(self.AAdendminmax)) + + #self.AAdendminmax = self.AAdendminmax + self.AAdendminmax + self.AAdendminmax + elif syntype == 1: + print('PF syn number!!!!!!!!!!!!!!!!!!!!!!!!!!!', len(self.PFdendminmax)) + + #self.PFdendminmax = self.PFdendminmax + self.PFdendminmax + self.PFdendminmax + + elif syntype == 2: + print('STL syn number!!!!!!!!!!!!!!!!!!!!!!!!!!', len(self.SCdendminmax)) + +#new spine coordinator + + def spine_space_limit(self): + h.define_shape() + self.spinehead_coord_x = {} + self.spinehead_coord_y = {} + + print('checking the size of the morphology') + for i, j in enumerate(self.spine_head): + j.push() + self.spinehead_coord_x["x0_"+str(i)] = h.x3d(0) + self.spinehead_coord_y["y0_"+str(i)] = h.y3d(0) + h.pop_section() + + ##only of X + self.spine_space_x = sorted(self.spinehead_coord_x.values()) + + ##only of Y + self.spine_space_y = sorted(self.spinehead_coord_y.values()) + + def dendrites_xy(self,min_x, max_x, min_y,max_y): + self.dend_cood_x = {} + self.dend_cood_y = {} + for i, j in enumerate(self.dend): + j.push() + self.dend_cood_x["x0_"+str(i)] = h.x3d(0) + self.dend_cood_y["y0_"+str(i)] = h.y3d(0) + h.pop_section() + + #only of X + dend_x_values = sorted(self.dend_cood_x.values()) + dend_x_kyes = sorted(self.dend_cood_x, key=self.dend_cood_x.get) + dend_list_zip_x = zip(dend_x_kyes, dend_x_values) + + + #only of Y + dend_y_values = sorted(self.dend_cood_y.values()) + dend_y_kyes = sorted(self.dend_cood_y, key=self.dend_cood_y.get) + dend_list_zip_y = zip(dend_y_kyes, dend_y_values) + + + self.x_coord_dend = [] + xlist = [] + for i in dend_list_zip_x: + if i[1] > min_x and i[1] < max_x: + xlist.append(i[1]) + self.x_coord_dend.append(int(i[0][3:])) + + + self.y_coord_dend = [] + ylist = [] + for i in dend_list_zip_y: + if i[1] > min_y and i[1] < max_y: + ylist.append(i[1]) + self.y_coord_dend.append(int(i[0][3:])) + + self.total_spines = [] + self.total_spines = set(self.x_coord_dend).intersection(self.y_coord_dend) + + print('total_dend_SC', len(self.total_spines)) + + + #New code dend only on X and Y at the same time to gnerate spots of activation + def spine_heads_x_y(self,min_x, max_x, min_y,max_y): + h.define_shape() #gives pt3d x,v,z to all the spines + self.spinehead_coord_x = {} + self.spinehead_coord_y = {} + for i, j in enumerate(self.spine_head): + j.push() + self.spinehead_coord_x["x0_"+str(i)] = h.x3d(0) + self.spinehead_coord_y["y0_"+str(i)] = h.y3d(0) + h.pop_section() + + #only of X + spine_x_values = sorted(self.spinehead_coord_x.values()) #sorting of the values + spine_x_kyes = sorted(self.spinehead_coord_x, key=self.spinehead_coord_x.get) #sorting of the keys based on the values + spine_list_zip_x = zip(spine_x_kyes, spine_x_values) + + + #only of Y + spine_y_values = sorted(self.spinehead_coord_y.values()) #sorting of the values + spine_y_kyes = sorted(self.spinehead_coord_y, key=self.spinehead_coord_y.get) #sorting of the keys based on the values + spine_list_zip_y = zip(spine_y_kyes, spine_y_values) + + + self.x_coord_spinehead = [] + xlist = [] + for i in spine_list_zip_x: + if i[1] > min_x and i[1] < max_x: + xlist.append(i[1]) + self.x_coord_spinehead.append(int(i[0][3:])) + + + self.y_coord_spinehead = [] + ylist = [] + for i in spine_list_zip_y: + if i[1] > min_y and i[1] < max_y: + ylist.append(i[1]) + self.y_coord_spinehead.append(int(i[0][3:])) + + self.total_spines = [] + self.total_spines = set(self.x_coord_spinehead).intersection(self.y_coord_spinehead) + + print('total_spines', len(self.total_spines)) + + + + + def activator(self, syntype, option_random, n_aa_set, n_pf_set, n_aa_sc_set, n_pf_sc_set, n_aa_aa_set): + print('spine synapses ON.') + +#AA only with random and fixed + self.AAdendminmax = [] + self.aarand = [] + self.aarand_spine_num = [] + self.AA_total = [] + + if syntype == 0: + for sec_name_value_AA in self.spine_head_aa: + for y in self.total_spines: + if 'spine_head_' + str(y) == str(sec_name_value_AA): + if option_random == 0: + self.AAdendminmax.append(Synapse_py3('aa',self,sec_name_value_AA)) + self.aarand_spine_num.append(self.sec_name_value_AA.name()) + else: + self.AA_total.append(self.sec_name_value_AA) + + if option_random == 1: + + naa = len(self.AA_total) + for z in range(int((naa/100)*n_aa_set)): + naa -= 1 + i = random.randint(0, naa) + self.AA_total[i], self.AA_total[naa] = self.AA_total[naa], self.AA_total[i] + self.aarand.append(self.AA_total[naa]) + self.AAdendminmax.append(Synapse_py3('aa',self,self.self.aarand[z])) + self.aarand_spine_num.append(self.aarand[z].name()) + + + +#PF only with random and fixed + self.PFdendminmax = [] + self.pfrand = [] + self.pfrand_spine_num = [] + self.PF_total = [] + + if syntype == 1: + for sec_name_value_PF in self.spine_head_pf: + for y in self.total_spines: + if 'spine_head_' + str(y) == str(sec_name_value_PF): + if option_random == 0: + self.PFdendminmax.append(Synapse_py3('pf',self,sec_name_value_PF)) + self.pfrand_spine_num.append(sec_name_value_PF.name()) + + else: + self.PF_total.append(sec_name_value_PF) + + if option_random == 1: + npf = len(self.PF_total) + for z in range(int((npf/100)*n_pf_set)): + npf -= 1 + i = random.randint(0, npf) + self.PF_total[i], self.PF_total[npf] = self.PF_total[npf], self.PF_total[i] + self.pfrand.append(self.PF_total[npf]) + self.PFdendminmax.append(Synapse_py3('pf',self,self.pfrand[z])) + self.pfrand_spine_num.append(self.pfrand[z].name()) + +#SC on AA only with random and fixed + self.SC_AAdendminmax = [] + self.SC_AA_rand = [] + self.SC_AA_spine_num = [] + self.SC_AA_total = [] + if syntype == 2: + for sec_name_value_SC_AA in self.dendAA_SC_index: + for y in self.total_spines: + if str(y) == str(sec_name_value_SC_AA): + if option_random == 0: + self.SC_AAdendminmax.append(Synapse_py3('stl',self,self.dend[sec_name_value_SC_AA])) + self.SC_AA_spine_num.append(sec_name_value_SC_AA) + else: + self.SC_AA_total.append(self.dend[sec_name_value_SC_PF]) + + if option_random == 1: + naasc = len(self.SC_AA_total) + for z in range(int((naasc/100)*n_aa_sc_set)): + naasc -= 1 + i = random.randint(0, naasc) + self.SC_AA_total[i], self.SC_AA_total[naasc] = self.SC_AA_total[naasc], self.SC_AA_total[i] + self.SC_AA_rand.append(self.SC_AA_total[naasc]) + self.SC_AAdendminmax.append(Synapse_py3('stl',self,self.SC_AA_rand[z])) + self.SC_AA_spine_num.append(self.SC_AA_rand[z].name()) + +#SC on PF only with random and fixed + self.SC_PFdendminmax = [] + self.SC_PF_rand = [] + self.SC_PF_spine_num = [] + self.SC_PF_total = [] + + if syntype == 3: + for sec_name_value_SC_PF in self.dendPF_SC_index: + for y in self.total_spines: + if y == sec_name_value_SC_PF: + if option_random == 0: + #print('no random SC_PF') + self.SC_PFdendminmax.append(Synapse_py3('stl',self,self.dend[sec_name_value_SC_PF])) + self.SC_PF_spine_num.append(sec_name_value_SC_PF) + else: + self.SC_PF_total.append(self.dend[sec_name_value_SC_PF]) + + if option_random == 1: + naapf = len(self.SC_PF_total) + for z in range(int((naapf/100)*n_pf_sc_set)): + naapf -= 1 + i = random.randint(0, naapf) + self.SC_PF_total[i], self.SC_PF_total[naapf] = self.SC_PF_total[naapf], self.SC_PF_total[i] + self.SC_PF_rand.append(self.SC_PF_total[naapf]) + self.SC_PFdendminmax.append(Synapse_py3('stl',self,self.SC_PF_rand[z])) + self.SC_PF_spine_num.append(self.SC_PF_rand[z].name()) + + + +#AA only in SC location + self.AA_AAdendminmax = [] + self.AA_AA_rand = [] + self.AA_AA_spine_num = [] + self.AA_AA_total = [] + + if syntype == 4: + for sec_name_value_AA_AA in self.spine_head_aa_SC: + for y in self.total_spines: + if 'spine_head_' + str(y) == str(sec_name_value_AA_AA): + if option_random == 0: + self.AA_AAdendminmax.append(Synapse_py3('aa',self,sec_name_value_AA_AA)) + self.AA_AA_spine_num.append(sec_name_value_AA_AA.name()) + else: + self.AA_AA_total.append(sec_name_value_AA_AA) + + if option_random == 1: + naa_aasc = len(self.AA_AA_total) + for z in range(int((naa_aasc/100)*n_aa_aa_set)): + naa_aasc -= 1 + i = random.randint(0, naa_aasc) + self.AA_AA_total[i], self.AA_AA_total[naa_aasc] = self.AA_AA_total[naa_aasc], self.AA_AA_total[i] + self.AA_AA_rand.append(self.AA_AA_total[naa_aasc]) + self.AA_AAdendminmax.append(Synapse_py3('aa',self,self.AA_AA_rand[z])) + self.AA_AA_spine_num.append(self.AA_AA_rand[z].name()) + diff --git a/docs/tutorial/01_opt_external_models/Purkinje_morpho_1_number.py b/docs/tutorial/01_opt_external_models/Purkinje_morpho_1_number.py new file mode 100644 index 00000000..54d79c54 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/Purkinje_morpho_1_number.py @@ -0,0 +1,6 @@ +number_ind_1 = dict() +number_ind_1['indiv'] = 138 +number_ind_1['morfo_n'] = 1 + + + diff --git a/docs/tutorial/01_opt_external_models/R_01_final_pop.txt b/docs/tutorial/01_opt_external_models/R_01_final_pop.txt new file mode 100644 index 00000000..200a62fc --- /dev/null +++ b/docs/tutorial/01_opt_external_models/R_01_final_pop.txt @@ -0,0 +1,576 @@ +0.01571631487541 0.00121504393028 0.00015181081724 0.01113971955228 0.00090913988455 0.00001443231345 0.00132550449949 0.00000461599317 0.00176140530165 0.00019009085054 0.03296487032394 0.00109084501250 0.00566709120465 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0.00000705345611 0.00099388809579 0.00014082725894 0.01163855091786 0.00148652168657 0.00735229700133 0.00091820524516 0.76574618543479 0.00980579170740 0.00018627088852 0.00000609168341 0.01751419767927 0.03596627460944 0.01270855958966 0.00020388112695 0.00001161562771 0.00045602628309 diff --git a/docs/tutorial/01_opt_external_models/Synapses.py b/docs/tutorial/01_opt_external_models/Synapses.py new file mode 100644 index 00000000..318dc968 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/Synapses.py @@ -0,0 +1,63 @@ +from neuron import h + +class Synapse_py3: + def __init__(self,source,target,section,weight = 1): + + self.input = h.NetStim(0.5) + self.input.start = -10 + self.input.number = 1 + self.input.interval = 1e9 + self.weight = weight + + + self.postsyns = {} + + if (type(source) == type('s')): + sourcetype = source + + +#Purkinje cell + if sourcetype == 'pf': + if target.whatami == 'prk': + # Make a PF synapse onto a purkinje cell + # Use deterministic synapses* + self.whatami = "syn_pf2prk_det" + self.postsyns['AMPA'] = [h.PF_syn(0.5, sec=section)] + self.postsyns['AMPA'][0].tau_facil=10.8*5# + self.postsyns['AMPA'][0].tau_rec=35.1 + self.postsyns['AMPA'][0].tau_1=3*2 + self.postsyns['AMPA'][0].gmax = 2800 + self.postsyns['AMPA'][0].U=0.13 + + self.nc_syn = [h.NetCon(self.input,receptor[0],0,0.1,1) for receptor in self.postsyns.values()] + + elif sourcetype == 'aa': + if target.whatami == 'prk': + # Make a ascending axon synapse onto a purkinje cell + # Use deterministic synapses + self.whatami = "syn_aa2prk_det" + self.postsyns['AMPA'] = [h.PF_syn(0.5, sec=section)] + self.postsyns['AMPA'][0].tau_facil=10.8*5 + self.postsyns['AMPA'][0].tau_rec=35.1*1 + self.postsyns['AMPA'][0].tau_1=3*5 + self.postsyns['AMPA'][0].gmax = 2800 + self.postsyns['AMPA'][0].U=0.13 + + self.nc_syn = [h.NetCon(self.input,receptor[0],0,0.1,1) for receptor in self.postsyns.values()] + + elif sourcetype == 'stl': + if target.whatami == 'prk': + self.whatami = "syn_stl2prk_alpha1" + self.postsyns['GABA'] = [h.PC_gaba_alpha1(0.5, sec=section)] # self.postsyns + self.postsyns['GABA'][0].tau_facil=4 + self.postsyns['GABA'][0].tau_rec=15 + self.postsyns['GABA'][0].tau_1=1 + self.postsyns['GABA'][0].Erev = -60 + self.postsyns['GABA'][0].gmaxA1 = 2600 + self.postsyns['GABA'][0].U=0.35 + self.nc_syn = [h.NetCon(self.input,receptor[0],0,0.1,1) for receptor in self.postsyns.values()] + + else: + print('SOURCE TYPE DOES NOT EXIST SOMETHING WRONG!!!!!!!!!') + + diff --git a/docs/tutorial/01_opt_external_models/_static/_tutorial_images/modeldb_hay_screen.png b/docs/tutorial/01_opt_external_models/_static/_tutorial_images/modeldb_hay_screen.png new file mode 100755 index 00000000..3a10a762 Binary files /dev/null and b/docs/tutorial/01_opt_external_models/_static/_tutorial_images/modeldb_hay_screen.png differ diff --git a/docs/tutorial/01_opt_external_models/build_network.L5_updated.py b/docs/tutorial/01_opt_external_models/build_network.L5_updated.py new file mode 100644 index 00000000..e45ce0cb --- /dev/null +++ b/docs/tutorial/01_opt_external_models/build_network.L5_updated.py @@ -0,0 +1,43 @@ +from bmtk.builder.networks import NetworkBuilder + +net = NetworkBuilder('L5') +net.add_nodes( + N=5, + model_type='biophysical', + model_template='hoc:L5PCtemplate', + morphology='cell3.asc' +) + +net.add_nodes( + N=5, + model_type='biophysical', + model_template='hoc:HNTemplate', + morphology='HL5PN1.swc' +) + + +net.build() +net.save(output_dir='network_L5_updated') + + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=20, + model_type='virtual', + ei_type='exc' +) +conns = virt_exc.add_edges( + source=virt_exc.nodes(), + target=net.nodes(), + connection_rule=12, + model_template='Exp2Syn', + dynamics_params='AMPA_ExcToExc.json', + distance_range=[0.0, 1.0e20], + target_sections=['soma', 'basal', 'apical'], + delay=2.0, + syn_weight=0.01 +) + +virt_exc.build() +virt_exc.save(output_dir='network_L5_updated') \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/build_network.added_channels.py b/docs/tutorial/01_opt_external_models/build_network.added_channels.py new file mode 100644 index 00000000..6f8e7efb --- /dev/null +++ b/docs/tutorial/01_opt_external_models/build_network.added_channels.py @@ -0,0 +1,36 @@ +from bmtk.builder.networks import NetworkBuilder + +net = NetworkBuilder('L5') +net.add_nodes( + N=1, + model_type='biophysical', + model_template='hoc:L5PCtemplate', + morphology='cell3.asc', + model_processing='add_channels' +) + + +net.build() +net.save(output_dir='network_added_channels') + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=20, + model_type='virtual', + ei_type='exc' +) +conns = virt_exc.add_edges( + source=virt_exc.nodes(), + target=net.nodes(), + connection_rule=12, + model_template='Exp2Syn', + dynamics_params='AMPA_ExcToExc.json', + distance_range=[0.0, 1.0e20], + target_sections=['soma', 'basal', 'apical'], + delay=2.0, + syn_weight=0.01 +) + +virt_exc.build() +virt_exc.save(output_dir='network_added_channels') \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/build_network.ball_and_stick.py b/docs/tutorial/01_opt_external_models/build_network.ball_and_stick.py new file mode 100644 index 00000000..d2d11de5 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/build_network.ball_and_stick.py @@ -0,0 +1,42 @@ +import numpy as np +from bmtk.builder.networks import NetworkBuilder + + +net = NetworkBuilder('BAS') +net.add_nodes( + N=5, + model_type='biophysical', + model_template='python:loadBAS', + gnabar=np.random.uniform(0.10, 0.15, size=5), + gkbar=np.random.uniform(0.02, 0.04, size=5), + gl=np.random.uniform(0.0001, 0.0005, size=5), + el=np.random.uniform(-80.0, -50.0, size=5), + g_pas = 0.001, + e_pas = -65 +) + + +net.build() +net.save(output_dir='network_BAS') + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=20, + model_type='virtual', + ei_type='exc' +) +conns = virt_exc.add_edges( + source=virt_exc.nodes(), + target=net.nodes(), + connection_rule=12, + model_template='Exp2Syn', + dynamics_params='AMPA_ExcToExc.json', + distance_range=[0.0, 1.0e20], + target_sections=['soma', 'dend'], + delay=1.0, + syn_weight=0.05 +) + +virt_exc.build() +virt_exc.save(output_dir='network_BAS') \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/build_network.modified_densities.py b/docs/tutorial/01_opt_external_models/build_network.modified_densities.py new file mode 100644 index 00000000..9585b3b5 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/build_network.modified_densities.py @@ -0,0 +1,36 @@ +from bmtk.builder.networks import NetworkBuilder + +net = NetworkBuilder('L5') +net.add_nodes( + N=1, + model_type='biophysical', + model_template='hoc:L5PCtemplate', + morphology='cell3.asc', + model_processing='adjust_densities' +) + + +net.build() +net.save(output_dir='network_modified_densities') + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=20, + model_type='virtual', + ei_type='exc' +) +conns = virt_exc.add_edges( + source=virt_exc.nodes(), + target=net.nodes(), + connection_rule=12, + model_template='Exp2Syn', + dynamics_params='AMPA_ExcToExc.json', + distance_range=[0.0, 1.0e20], + target_sections=['soma', 'basal', 'apical'], + delay=2.0, + syn_weight=0.01 +) + +virt_exc.build() +virt_exc.save(output_dir='network_modified_densities') \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/build_network.purkinje.py b/docs/tutorial/01_opt_external_models/build_network.purkinje.py new file mode 100644 index 00000000..6887be23 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/build_network.purkinje.py @@ -0,0 +1,45 @@ +import os +import numpy as np + +from bmtk.builder.networks import NetworkBuilder + + +np.random.seed(100) + +net = NetworkBuilder('purkinje') +net.add_nodes( + pop_name='Scnn1a', + model_type='biophysical', + model_template='python:Purkinje_morph_1', + spines_on=0 +) +net.add_nodes( + pop_name='Scnn1a', + model_type='biophysical', + model_template='python:Purkinje_morph_1', + spines_on=1 +) + + +net.build() +net.save(output_dir='network_Purkinje') + + +virt = NetworkBuilder('virt_exc') +virt.add_nodes( + N=5, + model_type='virtual' +) +virt.add_edges( + source=virt.nodes(), target=net.nodes(), + connection_rule=12, + syn_weight=6.4e-05, + distance_range=[50.0, 10.0e20], + target_sections=['dend'], + delay=1.0, + dynamics_params='AMPA_ExcToExc.json', + model_template='exp2syn' +) + +virt.build() +virt.save(output_dir='network_Purkinje') diff --git a/docs/tutorial/01_opt_external_models/components/dLGN_mechanisms/modfiles/iahp.mod b/docs/tutorial/01_opt_external_models/components/dLGN_mechanisms/modfiles/iahp.mod new file mode 100755 index 00000000..1c6d487b --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/dLGN_mechanisms/modfiles/iahp.mod @@ -0,0 +1,96 @@ +TITLE Medium duration Ca-dependent potassium current +: +: Ca++ dependent K+ current IC responsible for medium duration AHP +: +: Original file written by Alain Destexhe, Salk Institute, Nov 3, 1992 +: Modified by Geir Halnes, Norwegian University of Life Sciences, Mar 13, 2011 + + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +NEURON { + SUFFIX iahp + USEION k READ ek WRITE ik VALENCE 1 + USEION Ca READ Cai VALENCE 2 + RANGE gkbar, g, minf, taum + GLOBAL beta, cac, m_inf, tau_m, x +} + + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) + (molar) = (1/liter) + (mM) = (millimolar) +} + + +PARAMETER { + v (mV) + ek = -90 (mV) + celsius = 36 (degC) + Cai = 5e-5 (mM) : Initial [Ca]i = 50 nM (Cai is simulated by separate mod-file) + gkbar = 1.3e-4 (mho/cm2) : Conductance (modified from hoc-file) + beta = 0.02 (1/ms) : Backward rate constant + cac = 4.3478e-4(mM) : Middle point of m_inf fcn + taumin = 1 (ms) : Minimal value of the time cst + x = 2 : Binding cites +} + + + + +STATE { + m +} + + +ASSIGNED { + ik (mA/cm2) + g (mho/cm2) + m_inf + tau_m (ms) + minf + taum + tadj +} + + +BREAKPOINT { + SOLVE states METHOD cnexp + minf = m_inf + taum = tau_m + g = gkbar*m*m + ik = g * (v - ek) +} + +DERIVATIVE states { + evaluate_fct(v,Cai) + m' = (m_inf - m) / tau_m +} + + +UNITSOFF +INITIAL { +: activation kinetics are assumed to be at 22 deg. C +: Q10 is assumed to be 3 + + VERBATIM + Cai = _ion_Cai; + ENDVERBATIM + + tadj = 3 ^ ((celsius-22.0)/10) + evaluate_fct(v,Cai) + m = m_inf + minf = m_inf + taum = tau_m +} + +PROCEDURE evaluate_fct(v(mV),Cai(mM)) { LOCAL car, tcar + car = (Cai/cac)^x + m_inf = car / ( 1 + car ) + tau_m = 1 / beta / (1 + car) / tadj + if(tau_m < taumin) { tau_m = taumin } : min value of time cst +} + +UNITSON diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/CaDynamics.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/CaDynamics.mod new file mode 100644 index 00000000..d66cdb6c --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/CaDynamics.mod @@ -0,0 +1,40 @@ +: Dynamics that track inside calcium concentration +: modified from Destexhe et al. 1994 + +NEURON { + SUFFIX CaDynamics + USEION ca READ ica WRITE cai + RANGE decay, gamma, minCai, depth +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + FARADAY = (faraday) (coulombs) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) +} + +PARAMETER { + gamma = 0.05 : percent of free calcium (not buffered) + decay = 80 (ms) : rate of removal of calcium + depth = 0.1 (um) : depth of shell + minCai = 1e-4 (mM) +} + +ASSIGNED {ica (mA/cm2)} + +INITIAL { + cai = minCai +} + +STATE { + cai (mM) + } + +BREAKPOINT { SOLVE states METHOD cnexp } + +DERIVATIVE states { + cai' = -(10000)*(ica*gamma/(2*FARADAY*depth)) - (cai - minCai)/decay +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/CaDynamics_E2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/CaDynamics_E2.mod new file mode 100755 index 00000000..dd4337ff --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/CaDynamics_E2.mod @@ -0,0 +1,36 @@ +: Dynamics that track inside calcium concentration +: modified from Destexhe et al. 1994 + +NEURON { + SUFFIX CaDynamics_E2 + USEION ca READ ica WRITE cai + RANGE decay, gamma, minCai, depth +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + FARADAY = (faraday) (coulombs) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) +} + +PARAMETER { + gamma = 0.05 : percent of free calcium (not buffered) + decay = 80 (ms) : rate of removal of calcium + depth = 0.1 (um) : depth of shell + minCai = 1e-4 (mM) +} + +ASSIGNED {ica (mA/cm2)} + +STATE { + cai (mM) + } + +BREAKPOINT { SOLVE states METHOD cnexp } + +DERIVATIVE states { + cai' = -(10000)*(ica*gamma/(2*FARADAY*depth)) - (cai - minCai)/decay +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_HVA.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_HVA.mod new file mode 100644 index 00000000..fbf55470 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_HVA.mod @@ -0,0 +1,72 @@ +:Comment : +:Reference : : Reuveni, Friedman, Amitai, and Gutnick, J.Neurosci. 1993 + +NEURON { + SUFFIX Ca_HVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + UNITSOFF + if((v == -27) ){ + v = v+0.0001 + } + mAlpha = (0.055*(-27-v))/(exp((-27-v)/3.8) - 1) + mBeta = (0.94*exp((-75-v)/17)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + hAlpha = (0.000457*exp((-13-v)/50)) + hBeta = (0.0065/(exp((-v-15)/28)+1)) + hInf = hAlpha/(hAlpha + hBeta) + hTau = 1/(hAlpha + hBeta) + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_LVA.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_LVA.mod new file mode 100644 index 00000000..11d0b3f4 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_LVA.mod @@ -0,0 +1,68 @@ +:Comment : LVA ca channel. Note: mtau is an approximation from the plots +:Reference : : Avery and Johnston 1996, tau from Randall 1997 +:Comment: shifted by -10 mv to correct for junction potential +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Ca_LVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1.0000/(1+ exp((v - -30.000)/-6)) + mTau = (5.0000 + 20.0000/(1+exp((v - -25.000)/5)))/qt + hInf = 1.0000/(1+ exp((v - -80.000)/6.4)) + hTau = (20.0000 + 50.0000/(1+exp((v - -40.000)/7)))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_LVAst.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_LVAst.mod new file mode 100755 index 00000000..9226d15c --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ca_LVAst.mod @@ -0,0 +1,68 @@ +:Comment : LVA ca channel. Note: mtau is an approximation from the plots +:Reference : : Avery and Johnston 1996, tau from Randall 1997 +:Comment: shifted by -10 mv to correct for junction potential +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Ca_LVAst + USEION ca READ eca WRITE ica + RANGE gCa_LVAstbar, gCa_LVAst, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gCa_LVAstbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + gCa_LVAst (S/cm2) + mInf + mTau (ms) + hInf + hTau (ms) +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gCa_LVAst = gCa_LVAstbar*m*m*h + ica = gCa_LVAst*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1.0000/(1+ exp((v - -30.000)/-6)) + mTau = (5.0000 + 20.0000/(1+exp((v - -25.000)/5)))/qt + hInf = 1.0000/(1+ exp((v - -80.000)/6.4)) + hTau = (20.0000 + 50.0000/(1+exp((v - -40.000)/7)))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav2_1.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav2_1.mod new file mode 100644 index 00000000..65aacf8d --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav2_1.mod @@ -0,0 +1,133 @@ +TITLE P-type calcium channel + +COMMENT + +Constructed from the recording data provided by Bruce Bean. +Reference: Swensen AM and Bean BP (2005) Robustness of burst firing in dissociated purkinje neurons with acute or long-term reductions in sodium conductance. J Neurosci 25:3509-20 + +Current Model Reference: Anwar H, Hong S, De Schutter E (2010) Controlling Ca2+-activated K+ channels with models of Ca2+ buffering in Purkinje cell. Cerebellum* + +*Article available as Open Access + +PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20981513 + + +Written by Sungho Hong, Computational Neuroscience Unit, Okinawa Institute of Science and Technology, 2009. +Contact: Sungho Hong (shhong@oist.jp) + +Suffix from newCaP to Cav2_1 + + +ENDCOMMENT + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +NEURON { + SUFFIX Cav2_1 + USEION ca READ cai, cao WRITE ica + RANGE pcabar, ica, gk, vhalfm, cvm, vshift, taum, minf +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (nA) = (nanoamp) + (pA) = (picoamp) + (S) = (siemens) + (nS) = (nanosiemens) + (pS) = (picosiemens) + (um) = (micron) + (molar) = (1/liter) + (mM) = (millimolar) +} + +CONSTANT { + q10 = 3 + F = 9.6485e4 (coulombs) + R = 8.3145 (joule/kelvin) +} + +PARAMETER { + v (mV) + celsius (degC) + + cai (mM) + cao (mM) + + vhalfm = -29.458 (mV) + cvm = 8.429(mV) + vhalfh = -11.039 (mV) + cvh = 16.098 (mV) + vshift = 0 (mV) + + pcabar = 2.2e-4 (cm/s) +} + +ASSIGNED { + qt + ica (mA/cm2) + minf + taum (ms) + gk (coulombs/cm3) + T (kelvin) + E (volt) + zeta +} + +STATE { m } + +INITIAL { + qt = q10^((celsius-23 (degC))/10 (degC)) + T = kelvinfkt( celsius ) + rates(v) + m = minf +} + +BREAKPOINT { + SOLVE states METHOD cnexp + + ica = (1e3) * pcabar * m * m * m * gk +} + +DERIVATIVE states { + rates(v) + m' = (minf-m)/taum +} + +FUNCTION ghk( v (mV), ci (mM), co (mM), z ) (coulombs/cm3) { + E = (1e-3) * v + zeta = (z*F*E)/(R*T) + + if ( fabs(1-exp(-zeta)) < 1e-6 ) { + ghk = (1e-6) * (z*F) * (ci - co*exp(-zeta)) * (1 + zeta/2) + } else { + ghk = (1e-6) * (z*zeta*F) * (ci - co*exp(-zeta)) / (1-exp(-zeta)) + } +} + +PROCEDURE rates( v (mV) ) { + + minf = 1 / ( 1 + exp(-(v-vhalfm-vshift)/cvm) ) + + taum = taumfkt(v-vshift)/qt + + gk = ghk(v-vshift, cai, cao, 2) +} + + +FUNCTION kelvinfkt( t (degC) ) (kelvin) { + UNITSOFF + kelvinfkt = 273.19 + t + UNITSON +} + +FUNCTION taumfkt( v (mV) ) (ms) { + UNITSOFF + if (v>=-40) { + taumfkt = 0.2702 + 1.1622 * exp(-(v+26.798)*(v+26.798)/164.19) + } else { + taumfkt = 0.6923 * exp(v/1089.372) + } + UNITSON +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_1.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_1.mod new file mode 100644 index 00000000..5231a538 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_1.mod @@ -0,0 +1,144 @@ +TITLE Low threshold calcium current Cerebellum Purkinje Cell Model + +COMMENT + +Kinetics adapted to fit the Cav3.1 Iftinca et al 2006, Temperature dependence of T-type Calcium channel gating, NEUROSCIENCE + +Reference: Anwar H, Hong S, De Schutter E (2010) Controlling Ca2+-activated K+ channels with models of Ca2+ buffering in Purkinje cell. Cerebellum* + +*Article available as Open Access + +PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20981513 + +Written by Haroon Anwar, Computational Neuroscience Unit, Okinawa Institute of Science and Technology, 2010. +Contact: Haroon Anwar (anwar@oist.jp) + +Suffix from CaT3_1 to CaV3_1 + +ENDCOMMENT + + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +NEURON { + SUFFIX Cav3_1 + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE g, pcabar, minf, taum, hinf, tauh + RANGE ica, m ,h + + } + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) + +} + +CONSTANT { + F = 9.6485e4 (coulombs) + R = 8.3145 (joule/kelvin) + q10 = 3 +} + +PARAMETER { + v (mV) + celsius (degC) + eca (mV) + pcabar = 2.5e-4 (cm/s) + cai (mM) : adjusted for eca=120 mV + cao (mM) + + v0_m_inf = -52 (mV) + v0_h_inf = -72 (mV) + k_m_inf = -5 (mV) + k_h_inf = 7 (mV) + + C_tau_m = 1 + A_tau_m = 1.0 + v0_tau_m1 = -40 (mV) + v0_tau_m2 = -102 (mV) + k_tau_m1 = 9 (mV) + k_tau_m2 = -18 (mV) + + C_tau_h = 15 + A_tau_h = 1.0 + v0_tau_h1 = -32 (mV) + k_tau_h1 = 7 (mV) + + } + + +STATE { + m h +} + +ASSIGNED { + ica (mA/cm2) + g (coulombs/cm3) + minf + taum (ms) + hinf + tauh (ms) + T (kelvin) + E (volt) + zeta + qt +} + +BREAKPOINT { + SOLVE castate METHOD cnexp + + ica = (1e3) *pcabar*m*m *h * g +} + +DERIVATIVE castate { + evaluate_fct(v) + + m' = (minf - m) / taum + h' = (hinf - h) / tauh +} + +FUNCTION ghk( v (mV), ci (mM), co (mM), z ) (coulombs/cm3) { + E = (1e-3) * v + zeta = (z*F*E)/(R*T) + + + if ( fabs(1-exp(-zeta)) < 1e-6 ) { + ghk = (1e-6) * (z*F) * (ci - co*exp(-zeta)) * (1 + zeta/2) + } else { + ghk = (1e-6) * (z*zeta*F) * (ci - co*exp(-zeta)) / (1-exp(-zeta)) + } +} + + +UNITSOFF +INITIAL { + + T = kelvinfkt (celsius) + + evaluate_fct(v) + m = minf + h = hinf + qt = q10^((celsius-37 (degC))/10 (degC)) +} + +PROCEDURE evaluate_fct(v(mV)) { + + minf = 1.0 / ( 1 + exp((v - v0_m_inf)/k_m_inf) ) + hinf = 1.0 / ( 1 + exp((v - v0_h_inf)/k_h_inf) ) + if (v<=-90) { + taum = 1 + } else { + taum = ( C_tau_m + A_tau_m / (exp((v - v0_tau_m1)/ k_tau_m1) + exp((v - v0_tau_m2)/k_tau_m2))) / qt + } + tauh = ( C_tau_h + A_tau_h / exp((v - v0_tau_h1)/k_tau_h1) ) / qt + g = ghk(v, cai, cao, 2) +} + +FUNCTION kelvinfkt( t (degC) ) (kelvin) { + kelvinfkt = 273.19 + t +} + +UNITSON diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_2.mod new file mode 100644 index 00000000..bf7754f8 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_2.mod @@ -0,0 +1,103 @@ +TITLE Low threshold calcium current +: +: Ca++ current responsible for low threshold spikes (LTS) +: RETICULAR THALAMUS +: Differential equations +: +: Model of Huguenard & McCormick, J Neurophysiol 68: 1373-1383, 1992. +: +: Written by Alain Destexhe, Salk Institute, Sept 18, 1992 +: +: - Biophysical properties of the T current were from recordings of +: - human recombinant Cav3.2 T-channel in HEK-293 cells +: - see Vitko et al., J. Neurosci 25(19) :4844-4855, 2005 +: - Q10 and shift parameters are fixed +: +: +: Suffix from CaT3_2 to Cav3_2 + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +NEURON { + SUFFIX Cav3_2 + USEION ca READ cai, cao WRITE ica + RANGE gcabar, m_inf, tau_m, h_inf, tau_h, shift, i,ica +} + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) + + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +PARAMETER { + v (mV) + celsius = 36 (degC) +: eca = 120 (mV) + gcabar = .0008 (mho/cm2) + shift = 0 (mV) + cai = 2.4e-4 (mM) : adjusted for eca=120 mV + cao = 2 (mM) +} + +STATE { + m h +} + +ASSIGNED { + ica (mA/cm2) + carev (mV) + m_inf + tau_m (ms) + h_inf + tau_h (ms) + phi_m + phi_h + i (mA/cm2) +} + +BREAKPOINT { + SOLVE castate METHOD cnexp + carev = (1e3) * (R*(celsius+273.15))/(2*FARADAY) * log (cao/cai) + ica = gcabar * m*m*h * (v-carev) + i = ica : diagnostic i added to display the current +} + +DERIVATIVE castate { + evaluate_fct(v) + + m' = (m_inf - m) / tau_m + h' = (h_inf - h) / tau_h +} + +UNITSOFF +INITIAL { +: +: Activation functions and kinetics were obtained from +: Vitko et al., 2005 at 23-25 deg. +: Transformation to 36 deg assuming Q10 of 5 and 3 for m and h +: (as in Coulter et al., J Physiol 414: 587, 1989) +: + phi_m = 5 ^ (12/10) + phi_h = 3 ^ (12/10) + + evaluate_fct(v) + + m = m_inf + h = h_inf +} + +PROCEDURE evaluate_fct(v(mV)) { +: + + m_inf = 1.0 / ( 1 + exp(-(v+shift+54.8)/7.4) ) + h_inf = 1.0 / ( 1 + exp((v+shift+85.5)/7.18) ) + + tau_m = ( 1.9 + 1.0 / ( exp((v+shift+37.0)/11.9) + exp(-(v+shift+131.6)/21) ) ) / phi_m + tau_h = 13.7 + (1942 + exp((v+shift+164)/9.2)) / (1 + exp((v+shift+89.3)/3.7) ) / phi_h +} +UNITSON diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_3.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_3.mod new file mode 100644 index 00000000..bf8f2833 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Cav3_3.mod @@ -0,0 +1,96 @@ +TITLE CaV 3.3 CA3 hippocampal neuron + +COMMENT + Cell model: CA3 hippocampal neuron + + Created by jun xu @ Clancy Lab of Cornell University Medical College on 3/27/05 + + Geometry: single-compartment model modified on 04/19/07 + Xu J, Clancy CE (2008) Ionic mechanisms of endogenous bursting in CA3 hippocampal pyramidal neurons: + a model study. PLoS ONE 3:e2056- [PubMed] + +ENDCOMMENT + + + NEURON { + : CaT--alpha 1I CaV3.3 + SUFFIX Cav3_3 + USEION ca READ cai, cao WRITE ica + RANGE gCav3_3bar, pcabar, ica, tau_l, tau_n, n_inf, l_inf +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gCav3_3bar = 0.00001 (S/cm2) + vhalfn = -41.5 :mv + vhalfl = -69.8 + kn = 6.2 + kl = -6.1 + q10 = 2.3 + pcabar = 0.0001 : cm/s to check!!! + z= 2 + F = 96520 : Farady constant (coulomb/mol) + R = 8.3134 : gas constant (J/K.mol) + PI = 3.14 +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + gCav3_3 (S/cm2) + n_inf + tau_n + l_inf + tau_l + cai (mM) + cao (mM) + qt + T : absolute temperature (K) + ghk + w +} + +STATE { + n + l +} + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = gCav3_3bar*pcabar*n*n*l*ghk +} + +DERIVATIVE states { + rates() + n' = (n_inf-n)/tau_n + l' = (l_inf-l)/tau_l + } + +INITIAL{ + T = celsius+273.14 + qt = pow(q10,(celsius-28)/10) + rates() + n = n_inf + l = l_inf +} + +PROCEDURE rates(){ + n_inf = 1/(1+exp(-(v-vhalfn)/kn)) + l_inf = 1/(1+exp(-(v-vhalfl)/kl)) + + if (v > -60) { + tau_n = (7.2+0.02*exp(-v/14.7))/qt + tau_l = (79.5+2.0*exp(-v/9.3))/qt + }else{ + tau_n = (0.875*exp((v+120)/41))/qt + tau_l = 260/qt + } + + w = v*0.001*z*F/(R*T) + ghk = -0.001*z*F*(cao-cai*exp(w))*w/(exp(w)-1) +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Gfluct.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Gfluct.mod new file mode 100644 index 00000000..2d87676a --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Gfluct.mod @@ -0,0 +1,229 @@ +TITLE Fluctuating conductances + +COMMENT +----------------------------------------------------------------------------- + + Fluctuating conductance model for synaptic bombardment + ====================================================== + +THEORY + + Synaptic bombardment is represented by a stochastic model containing + two fluctuating conductances g_e(t) and g_i(t) descibed by: + + Isyn = g_e(t) * [V - E_e] + g_i(t) * [V - E_i] + d g_e / dt = -(g_e - g_e0) / tau_e + sqrt(D_e) * Ft + d g_i / dt = -(g_i - g_i0) / tau_i + sqrt(D_i) * Ft + + where E_e, E_i are the reversal potentials, g_e0, g_i0 are the average + conductances, tau_e, tau_i are time constants, D_e, D_i are noise diffusion + coefficients and Ft is a gaussian white noise of unit standard deviation. + + g_e and g_i are described by an Ornstein-Uhlenbeck (OU) stochastic process + where tau_e and tau_i represent the "correlation" (if tau_e and tau_i are + zero, g_e and g_i are white noise). The estimation of OU parameters can + be made from the power spectrum: + + S(w) = 2 * D * tau^2 / (1 + w^2 * tau^2) + + and the diffusion coeffient D is estimated from the variance: + + D = 2 * sigma^2 / tau + + +NUMERICAL RESOLUTION + + The numerical scheme for integration of OU processes takes advantage + of the fact that these processes are gaussian, which led to an exact + update rule independent of the time step dt (see Gillespie DT, Am J Phys + 64: 225, 1996): + + x(t+dt) = x(t) * exp(-dt/tau) + A * N(0,1) + + where A = sqrt( D*tau/2 * (1-exp(-2*dt/tau)) ) and N(0,1) is a normal + random number (avg=0, sigma=1) + + +IMPLEMENTATION + + This mechanism is implemented as a nonspecific current defined as a + point process. + + +PARAMETERS + + The mechanism takes the following parameters: + + E_e = 0 (mV) : reversal potential of excitatory conductance + E_i = -75 (mV) : reversal potential of inhibitory conductance + + g_e0 = 0.0121 (umho) : average excitatory conductance + g_i0 = 0.0573 (umho) : average inhibitory conductance + + std_e = 0.0030 (umho) : standard dev of excitatory conductance + std_i = 0.0066 (umho) : standard dev of inhibitory conductance + + tau_e = 2.728 (ms) : time constant of excitatory conductance + tau_i = 10.49 (ms) : time constant of inhibitory conductance + + +Gfluct2: conductance cannot be negative + + +REFERENCE + + Destexhe, A., Rudolph, M., Fellous, J-M. and Sejnowski, T.J. + Fluctuating synaptic conductances recreate in-vivo--like activity in + neocortical neurons. Neuroscience 107: 13-24 (2001). + + (electronic copy available at http://cns.iaf.cnrs-gif.fr) + + + A. Destexhe, 1999 + +----------------------------------------------------------------------------- +ENDCOMMENT + + + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +NEURON { + POINT_PROCESS Gfluct2 + RANGE g_e, g_i, E_e, E_i, g_e0, g_i0, g_e1, g_i1 + RANGE std_e, std_i, tau_e, tau_i, D_e, D_i + RANGE new_seed + NONSPECIFIC_CURRENT i + THREADSAFE + POINTER donotuse +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + dt (ms) + + E_e = 0 (mV) : reversal potential of excitatory conductance + E_i = -75 (mV) : reversal potential of inhibitory conductance + + g_e0 = 0.0121 (umho) : average excitatory conductance + g_i0 = 0.0573 (umho) : average inhibitory conductance + + std_e = 0.0030 (umho) : standard dev of excitatory conductance + std_i = 0.0066 (umho) : standard dev of inhibitory conductance + + tau_e = 2.728 (ms) : time constant of excitatory conductance + tau_i = 10.49 (ms) : time constant of inhibitory conductance +} + +ASSIGNED { + v (mV) : membrane voltage + i (nA) : fluctuating current + g_e (umho) : total excitatory conductance + g_i (umho) : total inhibitory conductance + g_e1 (umho) : fluctuating excitatory conductance + g_i1 (umho) : fluctuating inhibitory conductance + D_e (umho umho /ms) : excitatory diffusion coefficient + D_i (umho umho /ms) : inhibitory diffusion coefficient + exp_e + exp_i + amp_e (umho) + amp_i (umho) + donotuse +} + +INITIAL { + g_e1 = 0 + g_i1 = 0 + if(tau_e != 0) { + D_e = 2 * std_e * std_e / tau_e + exp_e = exp(-dt/tau_e) + amp_e = std_e * sqrt( (1-exp(-2*dt/tau_e)) ) + } + if(tau_i != 0) { + D_i = 2 * std_i * std_i / tau_i + exp_i = exp(-dt/tau_i) + amp_i = std_i * sqrt( (1-exp(-2*dt/tau_i)) ) + } +} + +BREAKPOINT { + SOLVE oup + if(tau_e==0) { + g_e = std_e * grand() + } + if(tau_i==0) { + g_i = std_i * grand() + } + g_e = g_e0 + g_e1 + if(g_e < 0) { g_e = 0 } + g_i = g_i0 + g_i1 + if(g_i < 0) { g_i = 0 } + i = g_e * (v - E_e) + g_i * (v - E_i) +} + + +PROCEDURE oup() { : use grand() + if(tau_e!=0) { + g_e1 = exp_e * g_e1 + amp_e * grand() + } + if(tau_i!=0) { + g_i1 = exp_i * g_i1 + amp_i * grand() + } +} + + +PROCEDURE new_seed(seed) { : procedure to set the seed + set_seed(seed) + VERBATIM + printf("Setting random generator with seed = %g\n", _lseed); + ENDVERBATIM +} + +VERBATIM +double nrn_random_pick(void* r); +void* nrn_random_arg(int argpos); +ENDVERBATIM + +FUNCTION grand() { +VERBATIM + if (_p_donotuse) { + /* + : Supports separate independent but reproducible streams for + : each instance. However, the corresponding hoc Random + : distribution MUST be set to Random.uniform(0,1) + */ + _lgrand = nrn_random_pick(_p_donotuse); + }else{ + /* only can be used in main thread */ + if (_nt != nrn_threads) { +hoc_execerror("multithread random in InUnif"," only via hoc Random"); + } +ENDVERBATIM + : the old standby. Cannot use if reproducible parallel sim + : independent of nhost or which host this instance is on + : is desired, since each instance on this cpu draws from + : the same stream + grand = normrand(0,1) +VERBATIM + } +ENDVERBATIM +} + +PROCEDURE noiseFromRandom() { +VERBATIM + { + void** pv = (void**)(&_p_donotuse); + if (ifarg(1)) { + *pv = nrn_random_arg(1); + }else{ + *pv = (void*)0; + } + } +ENDVERBATIM +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/HCN1_Angeloetal2007.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/HCN1_Angeloetal2007.mod new file mode 100644 index 00000000..793af2f2 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/HCN1_Angeloetal2007.mod @@ -0,0 +1,101 @@ +TITLE I-h HCN1 channel from Kamilla Angelo, Michael London,Soren R. Christensen, and Michael Hausser 2007 J. of Neurosci. +COMMENT + +We call it HCN1 as PC express only HCN1 Santoro et al. 2000 +:aggiunta di correzione per Q10 by ERICA GRANDI + +ENDCOMMENT + +NEURON { + SUFFIX HCN1 + USEION h READ eh WRITE ih VALENCE 1 + RANGE gbar, hinf,tauh,ratetau,ih + RANGE hinf,tauh,eh +} + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) +} + + +CONSTANT { + q10=3 +} + +PARAMETER { + v (mV) + : eh =-34.4 (mV) + gbar=.0001 (mho/cm2) + ratetau = 1 (ms) + rec_temp = 23 (deg) : we set it here at room temperature as in Angelo et al. they forogot tp mention the recording temperature + ljp = 9.3 (mV) : liquid_junction_potential + v_inf_half_noljp = -90.3 (mV) + v_inf_k = 9.67 (mV) + v_tau_const = 0.0018 (1) + v_tau_half1_noljp = -68 (mV) + v_tau_half2_noljp = -68 (mV) + v_tau_k1 = -22 (mv) + v_tau_k2 = 7.14 (mv) + } + +STATE { + h +} + +ASSIGNED { + eh (mV) + ih (mA/cm2) + hinf + tauh + celsius (deg) + v_inf_half (mV) + v_tau_half1 (mV) + v_tau_half2 (mV) + qt +} + +INITIAL { + + : ADD Q10 correction!!!!! FATTO!!! + qt = q10^((celsius-37 (degC))/10 (degC)) + v_inf_half = (v_inf_half_noljp - ljp) + v_tau_half1 = (v_tau_half1_noljp - ljp) + v_tau_half2 = (v_tau_half2_noljp - ljp) + + rate(v) + h=hinf +} + +BREAKPOINT { + SOLVE states METHOD cnexp + ih = h*gbar*(v-eh) +} + +DERIVATIVE states { + rate(v) + h' = (hinf - h)/tauh +} + +PROCEDURE rate(v (mV)) { + : hinf=1/( 1+exp((90+v)/9.67) ) + : tauh=ratetau*1/(0.0018*( exp((v+68)/-22) + exp((v+68)/7.14) )) + hinf = 1 / (1+exp( (v-v_inf_half) / v_inf_k) ) + tauh = (ratetau / (v_tau_const * ( exp( (v-v_tau_half1) / v_tau_k1) + exp( (v-v_tau_half2) / v_tau_k2) )))/qt +} + + + + + + + + + + + + + + + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ih.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ih.mod new file mode 100644 index 00000000..0e0c822d --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Ih.mod @@ -0,0 +1,73 @@ +:Comment : +:Reference : : Kole,Hallermann,and Stuart, J. Neurosci. 2006 + +NEURON { + SUFFIX Ih + NONSPECIFIC_CURRENT ihcn + RANGE gbar, g, ihcn, shift1, shift2, shift3, shift4, shift5, shift6 +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + ehcn = -45.0 (mV) + shift1 = 154.9 + shift2 = 11.9 + shift3 = 0 + shift4 = 33.1 + shift5 = 6.43 + shift6 = 193 +} + +ASSIGNED { + v (mV) + ihcn (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ihcn = g*(v-ehcn) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + if(v == -shift1){ + v = v + 0.0001 + } + if(shift4 == 0){ + shift4 = shift4 + 0.0001 + } + if(shift2 == 0){ + shift2 = shift2 + 0.0001 + } + mAlpha = 0.001*(shift5)*(v+shift1)/(exp((v+shift1)/(shift2))-1) + mBeta = 0.001*(shift6)*exp((v+shift3)/(shift4)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Im.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Im.mod new file mode 100644 index 00000000..14d8566c --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Im.mod @@ -0,0 +1,61 @@ +:Reference : : Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Im + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Im_v2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Im_v2.mod new file mode 100755 index 00000000..fc219f71 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Im_v2.mod @@ -0,0 +1,59 @@ +: Based on Im model of Vervaeke et al. (2006) + +NEURON { + SUFFIX Im_v2 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-30)/10) + mAlpha = 0.007 * exp( (6 * 0.4 * (v - (-48))) / 26.12 ) + mBeta = 0.007 * exp( (-6 * (1 - 0.4) * (v - (-48))) / 26.12 ) + + mInf = mAlpha / (mAlpha + mBeta) + mTau = (15 + 1 / (mAlpha + mBeta)) / qt +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_P.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_P.mod new file mode 100644 index 00000000..899a4240 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_P.mod @@ -0,0 +1,72 @@ +:Comment : The persistent component of the K current +:Reference : : Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 +:Comment : shifted -10 mv to correct for junction potential +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + + +NEURON { + SUFFIX K_P + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + UNITSOFF + v = v + 10 + mInf = (1/(1 + exp(-(v+1)/12))) + if(v<-50){ + mTau = (1.25+175.03*exp(-v * -0.026))/qt + }else{ + mTau = ((1.25+13*exp(-v*0.026)))/qt + } + hInf = 1/(1 + exp(-(v+54)/-11)) + hTau = (360+(1010+24*(v+55))*exp(-((v+75)/48)^2))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_Pst.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_Pst.mod new file mode 100755 index 00000000..a6e27461 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_Pst.mod @@ -0,0 +1,72 @@ +:Comment : The persistent component of the K current +:Reference : : Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 +:Comment : shifted -10 mv to correct for junction potential +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + + +NEURON { + SUFFIX K_Pst + USEION k READ ek WRITE ik + RANGE gK_Pstbar, gK_Pst, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gK_Pstbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gK_Pst (S/cm2) + mInf + mTau (ms) + hInf + hTau (ms) +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gK_Pst = gK_Pstbar*m*m*h + ik = gK_Pst*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + UNITSOFF + v = v + 10 + mInf = (1/(1 + exp(-(v+1)/12))) + if(v<-50){ + mTau = (1.25+175.03*exp(-v * -0.026))/qt + }else{ + mTau = ((1.25+13*exp(-v*0.026)))/qt + } + hInf = 1/(1 + exp(-(v+54)/-11)) + hTau = (360+(1010+24*(v+55))*exp(-((v+75)/48)^2))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_T.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_T.mod new file mode 100644 index 00000000..a82db648 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_T.mod @@ -0,0 +1,68 @@ +:Comment : The transient component of the K current +:Reference : : Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 +:Comment : shifted -10 mv to correct for junction potential +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX K_T + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*(m^4)*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1/(1 + exp(-(v+0)/19)) + mTau = (0.34+0.92*exp(-((v+71)/59)^2))/qt + hInf = 1/(1 + exp(-(v+66)/-10)) + hTau = (8+49*exp(-((v+73)/23)^2))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_Tst.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_Tst.mod new file mode 100755 index 00000000..9d2849af --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/K_Tst.mod @@ -0,0 +1,68 @@ +:Comment : The transient component of the K current +:Reference : : Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 +:Comment : shifted -10 mv to correct for junction potential +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX K_Tst + USEION k READ ek WRITE ik + RANGE gK_Tstbar, gK_Tst, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gK_Tstbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gK_Tst (S/cm2) + mInf + mTau (ms) + hInf + hTau (ms) +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gK_Tst = gK_Tstbar*(m^4)*h + ik = gK_Tst*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1/(1 + exp(-(v+0)/19)) + mTau = (0.34+0.92*exp(-((v+71)/59)^2))/qt + hInf = 1/(1 + exp(-(v+66)/-10)) + hTau = (8+49*exp(-((v+73)/23)^2))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca11.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca11.mod new file mode 100644 index 00000000..46bcd656 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca11.mod @@ -0,0 +1,187 @@ +TITLE Large conductance Ca2+ activated K+ channel mslo + +COMMENT + +Parameters from Cox et al. (1987) J Gen Physiol 110:257-81 (patch 1). + +Current Model Reference: Anwar H, Hong S, De Schutter E (2010) Controlling Ca2+-activated K+ channels with models of Ca2+ buffering in Purkinje cell. Cerebellum* + +*Article available as Open Access + +PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20981513 + + +Written by Sungho Hong, Okinawa Institute of Science and Technology, March 2009. +Contact: Sungho Hong (shhong@oist.jp) + +Suffix from mslo to Kca1_1 + +ENDCOMMENT + +NEURON { + SUFFIX Kca1_1 + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE g, gbar, ik + +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (kilocoulombs) + R = (k-mole) (joule/degC) +} + +CONSTANT { + q10 = 3 +} + +PARAMETER { + gbar = 0.01 (S/cm2) + + Qo = 0.73 + Qc = -0.67 + + k1 = 1.0e3 (/mM) + onoffrate = 1 (/ms) + + L0 = 1806 + Kc = 11.0e-3 (mM) + Ko = 1.1e-3 (mM) + + pf0 = 2.39e-3 (/ms) + pf1 = 7.0e-3 (/ms) + pf2 = 40e-3 (/ms) + pf3 = 295e-3 (/ms) + pf4 = 557e-3 (/ms) + + pb0 = 3936e-3 (/ms) + pb1 = 1152e-3 (/ms) + pb2 = 659e-3 (/ms) + pb3 = 486e-3 (/ms) + pb4 = 92e-3 (/ms) +} + +ASSIGNED { + : rates + c01 (/ms) + c12 (/ms) + c23 (/ms) + c34 (/ms) + o01 (/ms) + o12 (/ms) + o23 (/ms) + o34 (/ms) + f0 (/ms) + f1 (/ms) + f2 (/ms) + f3 (/ms) + f4 (/ms) + + c10 (/ms) + c21 (/ms) + c32 (/ms) + c43 (/ms) + o10 (/ms) + o21 (/ms) + o32 (/ms) + o43 (/ms) + b0 (/ms) + b1 (/ms) + b2 (/ms) + b3 (/ms) + b4 (/ms) + + v (mV) + cai (mM) + ek (mV) + ik (milliamp/cm2) + g (S/cm2) + celsius (degC) +} + +STATE { + C0 FROM 0 TO 1 + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + O0 FROM 0 TO 1 + O1 FROM 0 TO 1 + O2 FROM 0 TO 1 + O3 FROM 0 TO 1 + O4 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * (O0 + O1 + O2 + O3 + O4) + ik = g * (v - ek) +} + +INITIAL { +: rates(v, cai) +: SOLVE seqinitial + SOLVE activation STEADYSTATE sparse +} + +KINETIC activation { + rates(v, cai) + ~ C0 <-> C1 (c01,c10) + ~ C1 <-> C2 (c12,c21) + ~ C2 <-> C3 (c23,c32) + ~ C3 <-> C4 (c34,c43) + ~ O0 <-> O1 (o01,o10) + ~ O1 <-> O2 (o12,o21) + ~ O2 <-> O3 (o23,o32) + ~ O3 <-> O4 (o34,o43) + ~ C0 <-> O0 (f0 , b0) + ~ C1 <-> O1 (f1 , b1) + ~ C2 <-> O2 (f2 , b2) + ~ C3 <-> O3 (f3 , b3) + ~ C4 <-> O4 (f4 , b4) + +CONSERVE C0 + C1 + C2 + C3 + C4 + O0 + O1 + O2 + O3 + O4 = 1 +} + +PROCEDURE rates(v(mV), ca (mM)) { + LOCAL qt, alpha, beta + + qt = q10^((celsius-23 (degC))/10 (degC)) + + c01 = 4*ca*k1*onoffrate*qt + c12 = 3*ca*k1*onoffrate*qt + c23 = 2*ca*k1*onoffrate*qt + c34 = 1*ca*k1*onoffrate*qt + o01 = 4*ca*k1*onoffrate*qt + o12 = 3*ca*k1*onoffrate*qt + o23 = 2*ca*k1*onoffrate*qt + o34 = 1*ca*k1*onoffrate*qt + + c10 = 1*Kc*k1*onoffrate*qt + c21 = 2*Kc*k1*onoffrate*qt + c32 = 3*Kc*k1*onoffrate*qt + c43 = 4*Kc*k1*onoffrate*qt + o10 = 1*Ko*k1*onoffrate*qt + o21 = 2*Ko*k1*onoffrate*qt + o32 = 3*Ko*k1*onoffrate*qt + o43 = 4*Ko*k1*onoffrate*qt + + alpha = exp(Qo*FARADAY*v/R/(273.15 + celsius)) + beta = exp(Qc*FARADAY*v/R/(273.15 + celsius)) + + f0 = pf0*alpha*qt + f1 = pf1*alpha*qt + f2 = pf2*alpha*qt + f3 = pf3*alpha*qt + f4 = pf4*alpha*qt + + b0 = pb0*beta*qt + b1 = pb1*beta*qt + b2 = pb2*beta*qt + b3 = pb3*beta*qt + b4 = pb4*beta*qt +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca22.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca22.mod new file mode 100644 index 00000000..b477bd0f --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca22.mod @@ -0,0 +1,132 @@ +TITLE SK2 multi-state model Cerebellum Golgi Cell Model + +COMMENT + +Author:Sergio Solinas, Lia Forti, Egidio DAngelo +Based on data from: Hirschberg, Maylie, Adelman, Marrion J Gen Physiol 1998 +Last revised: May 2007 + +Published in: + Sergio M. Solinas, Lia Forti, Elisabetta Cesana, + Jonathan Mapelli, Erik De Schutter and Egidio D`Angelo (2008) + Computational reconstruction of pacemaking and intrinsic + electroresponsiveness in cerebellar golgi cells + Frontiers in Cellular Neuroscience 2:2 + +Suffix from SK2 to Kca2_2 + +ENDCOMMENT + +NEURON{ + SUFFIX Kca2_2 + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gkbar, g, ik, tcorr +} + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) + (molar) = (1/liter) + (mM) = (millimolar) +} + +PARAMETER { + celsius (degC) + cai (mM) + gkbar = 0.038 (mho/cm2) + Q10 = 3 (1) + diff = 3 (1) : diffusion factor + +: rates ca-indipendent + invc1 = 80e-3 ( /ms) + invc2 = 80e-3 ( /ms) + invc3 = 200e-3 ( /ms) + + invo1 = 1 ( /ms) + invo2 = 100e-3 ( /ms) + diro1 = 160e-3 ( /ms) + diro2 = 1.2 ( /ms) + +: rates ca-dipendent + dirc2 = 200 ( /ms-mM ) + dirc3 = 160 ( /ms-mM ) + dirc4 = 80 ( /ms-mM ) + +} + +ASSIGNED{ + v (mV) + ek (mV) + g (mho/cm2) + ik (mA/cm2) + invc1_t ( /ms) + invc2_t ( /ms) + invc3_t ( /ms) + invo1_t ( /ms) + invo2_t ( /ms) + diro1_t ( /ms) + diro2_t ( /ms) + dirc2_t ( /ms-mM) + dirc3_t ( /ms-mM) + dirc4_t ( /ms-mM) + tcorr (1) + + dirc2_t_ca ( /ms) + dirc3_t_ca ( /ms) + dirc4_t_ca ( /ms) +} + +STATE { + c1 + c2 + c3 + c4 + o1 + o2 +} + +BREAKPOINT{ + SOLVE kin METHOD sparse + g = gkbar*(o1+o2) :(mho/cm2) + ik = g*(v-ek) :(mA/cm2) +} + +INITIAL{ + rate(celsius) + SOLVE kin STEADYSTATE sparse +} + +KINETIC kin{ + rates(cai/diff) + ~c1<->c2 (dirc2_t_ca, invc1_t) + ~c2<->c3 (dirc3_t_ca, invc2_t) + ~c3<->c4 (dirc4_t_ca, invc3_t) + ~c3<->o1 (diro1_t, invo1_t) + ~c4<->o2 (diro2_t, invo2_t) + CONSERVE c1+c2+c3+c4+o2+o1=1 +} + +FUNCTION temper (Q10, celsius (degC)) { + temper = Q10^((celsius -23(degC)) / 10(degC)) +} + +PROCEDURE rates(cai(mM)){ + dirc2_t_ca = dirc2_t*cai + dirc3_t_ca = dirc3_t*cai + dirc4_t_ca = dirc4_t*cai +} + +PROCEDURE rate (celsius(degC)) { + tcorr = temper (Q10,celsius) + invc1_t = invc1*tcorr + invc2_t = invc2*tcorr + invc3_t = invc3*tcorr + invo1_t = invo1*tcorr + invo2_t = invo2*tcorr + diro1_t = diro1*tcorr + diro2_t = diro2*tcorr + dirc2_t = dirc2*tcorr + dirc3_t = dirc3*tcorr + dirc4_t = dirc4*tcorr +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca31.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca31.mod new file mode 100644 index 00000000..2fd47b8b --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kca31.mod @@ -0,0 +1,92 @@ +TITLE Calcium dependent potassium channel +: Implemented in Rubin and Cleland (2006) J Neurophysiology +: Parameters from Bhalla and Bower (1993) J Neurophysiology +: Adapted from /usr/local/neuron/demo/release/nachan.mod - squid +: by Andrew Davison, The Babraham Institute [Brain Res Bulletin, 2000] + +:Suffix from Kca3 to Kca3_1 + +NEURON { + THREADSAFE + SUFFIX Kca3_1 + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gkbar, ik, Yconcdep, Yvdep + RANGE Yalpha, Ybeta, tauY, Y_inf +} + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) + (molar) = (1/liter) + (mM) = (millimolar) +} + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +CONSTANT { + q10 = 3 +} + +PARAMETER { + v (mV) + dt (ms) + gkbar= 0.120 (mho/cm2) <0,1e9> + Ybeta = 0.05 (/ms) + cai (mM) := 1e-5 (mM) +} + + +STATE { + Y +} + +ASSIGNED { + ik (mA/cm2) + Yalpha (/ms) + Yvdep + Yconcdep (/ms) + tauY (ms) + Y_inf + ek (mV) + + qt +} + +INITIAL { + rate(v,cai) + Y = Yalpha/(Yalpha + Ybeta) + qt = q10^((celsius-37 (degC))/10 (degC)) +} + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gkbar*Y*(v - ek) +} + +DERIVATIVE state { + rate(v,cai) + Y' = Yalpha*(1-Y) - Ybeta*Y +} + +PROCEDURE rate(v(mV),cai(mM)) { + vdep(v) + concdep(cai) + Yalpha = Yvdep*Yconcdep + tauY = 1/(Yalpha + Ybeta) + Y_inf = Yalpha/(Yalpha + Ybeta) /qt +} + +PROCEDURE vdep(v(mV)) { + TABLE Yvdep FROM -100 TO 100 WITH 100 + Yvdep = exp((v*1(/mV)+70)/27) +} + +PROCEDURE concdep(cai(mM)) { + TABLE Yconcdep FROM 0 TO 0.01 WITH 1000 + if (cai < 0.01) { + Yconcdep = 500(/ms)*( 0.015-cai*1(/mM) )/( exp((0.015-cai*1(/mM))/0.0013) -1 ) + } else { + Yconcdep = 500(/ms)*0.005/( exp(0.005/0.0013) -1 ) + } +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kd.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kd.mod new file mode 100755 index 00000000..82cbe59a --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kd.mod @@ -0,0 +1,62 @@ +: Based on Kd model of Foust et al. (2011) + + +NEURON { + SUFFIX Kd + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * h + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h' = (hInf - h) / hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-23)/10) + mInf = 1 - 1 / (1 + exp((v - (-43)) / 8)) + mTau = 1 + hInf = 1 / (1 + exp((v - (-67)) / 7.3)) + hTau = 1500 +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kir23.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kir23.mod new file mode 100644 index 00000000..669e10cf --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kir23.mod @@ -0,0 +1,95 @@ +TITLE Cerebellum Granule Cell Model + +COMMENT + Reference: Theta-Frequency Bursting and Resonance in Cerebellar Granule Cells:Experimental + Evidence and Modeling of a Slow K+-Dependent Mechanism + Egidio D'Angelo,Thierry Nieus,Arianna Maffei,Simona Armano,Paola Rossi,Vanni Taglietti, + Andrea Fontana and Giovanni Naldi + +Suffix from Ubc_Kir to Kir2_3 +ENDCOMMENT + +NEURON { + SUFFIX Kir2_3 + USEION k READ ek WRITE ik + RANGE gkbar, ik, g, alpha_d, beta_d, ek + RANGE Aalpha_d, Kalpha_d, V0alpha_d + RANGE Abeta_d, Kbeta_d, V0beta_d + RANGE d_inf, tau_d +} + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) +} + +PARAMETER { + Aalpha_d = 0.13289 (/ms) + + + Kalpha_d = -24.3902 (mV) + + V0alpha_d = -83.94 (mV) + Abeta_d = 0.16994 (/ms) + + + Kbeta_d = 35.714 (mV) + + V0beta_d = -83.94 (mV) + v (mV) + gkbar = 0.0009 (mho/cm2) + ek (mV) + celsius = 30 (degC) +} + +STATE { + d +} + +ASSIGNED { + ik (mA/cm2) + d_inf + tau_d (ms) + g (mho/cm2) + alpha_d (/ms) + beta_d (/ms) +} + +INITIAL { + rate(v) + d = d_inf +} + +BREAKPOINT { + SOLVE states METHOD derivimplicit + g = gkbar*d + ik = g*(v - ek) + alpha_d = alp_d(v) + beta_d = bet_d(v) +} + +DERIVATIVE states { + rate(v) + d' =(d_inf - d)/tau_d +} + +FUNCTION alp_d(v(mV))(/ms) { LOCAL Q10 + Q10 = 3^((celsius-20(degC))/10(degC)) + alp_d = Q10*Aalpha_d*exp((v-V0alpha_d)/Kalpha_d) +} + +FUNCTION bet_d(v(mV))(/ms) { LOCAL Q10 + Q10 = 3^((celsius-20(degC))/10(degC)) + bet_d = Q10*Abeta_d*exp((v-V0beta_d)/Kbeta_d) +} + +PROCEDURE rate(v (mV)) {LOCAL a_d, b_d + TABLE d_inf, tau_d + DEPEND Aalpha_d, Kalpha_d, V0alpha_d, + Abeta_d, Kbeta_d, V0beta_d, celsius FROM -100 TO 100 WITH 200 + a_d = alp_d(v) + b_d = bet_d(v) + tau_d = 1/(a_d + b_d) + d_inf = a_d/(a_d + b_d) +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv11.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv11.mod new file mode 100644 index 00000000..a8ea3e27 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv11.mod @@ -0,0 +1,145 @@ +TITLE Voltage-gated low threshold potassium current from Kv1 subunits + +COMMENT + +NEURON implementation of a potassium channel from Kv1.1 subunits +Kinetical scheme: Hodgkin-Huxley m^4, no inactivation + +Experimental data taken from: +Human Kv1.1 expressed in xenopus oocytes: Zerr et al., J Neurosci 18, 2842, 2848, 1998 +Vhalf = -28.8 +- 2.3 mV; k = 8.1+- 0.9 mV + +The voltage dependency of the rate constants was approximated by: + +alpha = ca * exp(-(v+cva)/cka) +beta = cb * exp(-(v+cvb)/ckb) + +Parameters ca, cva, cka, cb, cvb, ckb +were determined from least square-fits to experimental data of G/Gmax(v) and tau(v). +Values are defined in the CONSTANT block. +Model includes calculation of Kv gating current + +Reference: Akemann et al., Biophys. J. (2009) 96: 3959-3976 + +Laboratory for Neuronal Circuit Dynamics +RIKEN Brain Science Institute, Wako City, Japan +http://www.neurodynamics.brain.riken.jp + +Date of Implementation: April 2007 +Contact: akemann@brain.riken.jp + +Suffix from Kv1 to Kv1_1 + +ENDCOMMENT + + +NEURON { + THREADSAFE + SUFFIX Kv1_1 + USEION k READ ek WRITE ik + NONSPECIFIC_CURRENT i + RANGE g, gbar, ik, i , igate, nc + RANGE ninf, taun + RANGE gateCurrent, gunit +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (nA) = (nanoamp) + (pA) = (picoamp) + (S) = (siemens) + (nS) = (nanosiemens) + (pS) = (picosiemens) + (um) = (micron) + (molar) = (1/liter) + (mM) = (millimolar) +} + +CONSTANT { + e0 = 1.60217646e-19 (coulombs) + q10 = 2.7 + + ca = 0.12889 (1/ms) + cva = 45 (mV) + cka = -33.90877 (mV) + + cb = 0.12889 (1/ms) + cvb = 45 (mV) + ckb = 12.42101 (mV) + + zn = 2.7978 (1) : valence of n-gate +} + +PARAMETER { + gateCurrent = 0 (1) : gating currents ON = 1 OFF = 0 + + gbar = 0.004 (S/cm2) <0,1e9> + gunit = 16 (pS) : unitary conductance +} + + +ASSIGNED { + celsius (degC) + v (mV) + + ik (mA/cm2) + i (mA/cm2) + igate (mA/cm2) + ek (mV) + g (S/cm2) + nc (1/cm2) : membrane density of channel + + ninf (1) + taun (ms) + alphan (1/ms) + betan (1/ms) + qt (1) +} + +STATE { n } + +INITIAL { + nc = (1e12) * gbar / gunit + qt = q10^((celsius-22 (degC))/10 (degC)) + rates(v) + n = ninf +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * n^4 + ik = g * (v - ek) + igate = nc * (1e6) * e0 * 4 * zn * ngateFlip() + + if (gateCurrent != 0) { + i = igate + } +} + +DERIVATIVE states { + rates(v) + n' = (ninf-n)/taun +} + +PROCEDURE rates(v (mV)) { + alphan = alphanfkt(v) + betan = betanfkt(v) + ninf = alphan/(alphan+betan) + taun = 1/(qt*(alphan + betan)) +} + +FUNCTION alphanfkt(v (mV)) (1/ms) { + alphanfkt = ca * exp(-(v+cva)/cka) +} + +FUNCTION betanfkt(v (mV)) (1/ms) { + betanfkt = cb * exp(-(v+cvb)/ckb) +} + +FUNCTION ngateFlip() (1/ms) { + ngateFlip = (ninf-n)/taun +} + + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv15.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv15.mod new file mode 100644 index 00000000..3b84f430 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv15.mod @@ -0,0 +1,118 @@ +TITLE Cardiac IKur current & nonspec cation current with identical kinetics +: Hodgkin - Huxley type channels, modified to fit IKur data from Feng et al Am J Physiol 1998 275:H1717 - H 1725 +: Suffix from Kv15 to Kv1_5 + +NEURON { + SUFFIX Kv1_5 + USEION k READ ek WRITE ik :,ki,ko + :USEION na READ nai,nao + :USEION no WRITE ino VALENCE 1: nonspecific cation current + RANGE gKur, ik, ino, Tauact, Tauinactf,Tauinacts, gnonspec, nao, nai, ko,ki + RANGE minf, ninf, uinf, mtau , ntau, utau +} + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) + (mM) = (milli/liter) + F = (faraday) (coulombs) + R = (k-mole) (joule/degC) +} + +PARAMETER { + gKur=0.13195e-3 (S/cm2) <0,1e9> + Tauact=1 (ms) + Tauinactf=1 (ms) + Tauinacts=1 (ms) + gnonspec=0 (S/cm2) <0,1e9> +} +STATE { + m n u +} + +ASSIGNED { + v (mV) + celsius (degC) : 37 + ik (mA/cm2) + minf ninf uinf + mtau (ms) + ntau (ms) + utau (ms) + ek (mV) + ino (mA/cm2) + ki (mM) + ko (mM) + nai (mM) + nao (mM) +} + +INITIAL { + rates(v) + m = minf + n = ninf + u = uinf +} + +BREAKPOINT { LOCAL z + z = (R*(celsius+273.15))/F + SOLVE states METHOD derivimplicit + ik = gKur*(0.1 + 1/(1 + exp(-(v - 15)/13)))*m*m*m*n*u*(v - ek) + ino=gnonspec*(0.1 + 1/(1 + exp(-(v - 15)/13)))*m*m*m*n*u*(v - z*log((nao+ko)/(nai+ki))) +} + +DERIVATIVE states { : exact when v held constant + rates(v) + m' = (minf - m)/mtau + n' = (ninf - n)/ntau + u' = (uinf - u)/utau +} + +UNITSOFF +FUNCTION alp(v(mV),i) { LOCAL q10 : order m n + v = v + q10 = 2.2^((celsius - 37)/10) + if (i==0) { + alp = q10*0.65/(exp(-(v + 10)/8.5) + exp(-(v - 30)/59)) + } else if (i==1) { + alp = 0.001*q10/(2.4 +10.9* exp(-(v + 90)/78)) + } + +} + +FUNCTION bet(v(mV),i) (/ms) { LOCAL q10 : order m n u + v = v + q10 = 2.2^((celsius - 37)/10) + if (i==0){ + bet = q10*0.65/(2.5 + exp((v + 82)/17)) + }else if (i==1){ + bet = q10*0.001*exp((v - 168)/16) + } +} + +FUNCTION ce(v(mV),i)(/ms) { : order m n u + v = v + + if (i==0) { + ce = 1/(1 + exp(-(v + 30.3)/9.6)) + }else if (i==1){ + ce = 1*(0.25+1/(1.35 + exp((v + 7)/14))) + + }else if (i==2){ + ce = 1*(0.1+1/(1.1 + exp((v + 7)/14))) + } +} + + +PROCEDURE rates(v) {LOCAL a,b,c : + + a = alp(v,0) b=bet(v,0) c = ce(v,0) + mtau = 1/(a + b)/3*Tauact + minf = c + a = alp(v,1) b=bet(v,1) c = ce(v,1) + ntau = 1/(a + b)/3*Tauinactf + ninf = c + c = ce(v,2) + uinf = c + utau = 6800*Tauinacts +} +UNITSON diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv2like.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv2like.mod new file mode 100755 index 00000000..1cbdf843 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv2like.mod @@ -0,0 +1,86 @@ +: Kv2-like channel +: Adapted from model implemented in Keren et al. 2005 +: Adjusted parameters to be similar to guangxitoxin-sensitive current in mouse CA1 pyramids from Liu and Bean 2014 + + +NEURON { + SUFFIX Kv2like + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mAlpha + mBeta + mTau + hInf + h1Tau + h2Tau +} + +STATE { + m + h1 + h2 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * m * (0.5 * h1 + 0.5 * h2) + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h1' = (hInf - h1) / h1Tau + h2' = (hInf - h2) / h2Tau +} + +INITIAL{ + rates() + m = mInf + h1 = hInf + h2 = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mAlpha = 0.12 * vtrap( -(v - 43), 11.0) + mBeta = 0.02 * exp(-(v + 1.27) / 120) + mInf = mAlpha / (mAlpha + mBeta) + mTau = 2.5 * (1 / (qt * (mAlpha + mBeta))) + + hInf = 1/(1 + exp((v + 58) / 11)) + h1Tau = (360 + (1010 + 23.7 * (v + 54)) * exp(-((v + 75) / 48)^2)) / qt + h2Tau = (2350 + 1380 * exp(-0.011 * v) - 210 * exp(-0.03 * v)) / qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv33.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv33.mod new file mode 100644 index 00000000..3d49d891 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv33.mod @@ -0,0 +1,141 @@ +TITLE Voltage-gated potassium channel from Kv3 subunits + +COMMENT +Voltage-gated potassium channel with high threshold and fast activation/deactivation kinetics + +KINETIC SCHEME: Hodgkin-Huxley (n^4) +n'= alpha * (1-n) - betha * n +g(v) = gbar * n^4 * ( v-ek ) + +The rate constants of activation (alpha) and deactivation (beta) were approximated by: + +alpha(v) = ca * exp(-(v+cva)/cka) +beta(v) = cb * exp(-(v+cvb)/ckb) + +Parameters can, cvan, ckan, cbn, cvbn, ckbn are given in the CONSTANT block. +Values derive from least-square fits to experimental data of G/Gmax(v) and taun(v) in Martina et al. J Neurophys. 97 (563-671, 2007. +Model includes a calculation of Kv gating current + +Reference: Akemann et al., Biophys. J. (2009) 96: 3959-3976 + +Laboratory for Neuronal Circuit Dynamics +RIKEN Brain Science Institute, Wako City, Japan +http://www.neurodynamics.brain.riken.jp + +Date of Implementation: April 2007 +Contact: akemann@brain.riken.jp + +Suffix from Kv3 to Kv3_3 + +ENDCOMMENT + + +NEURON { + THREADSAFE + SUFFIX Kv3_3 + USEION k READ ek WRITE ik + NONSPECIFIC_CURRENT i + RANGE gbar, g, ik, i, igate, nc + RANGE ninf, taun + RANGE gateCurrent, gunit +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (nA) = (nanoamp) + (pA) = (picoamp) + (S) = (siemens) + (mS) = (millisiemens) + (nS) = (nanosiemens) + (pS) = (picosiemens) + (um) = (micron) + (molar) = (1/liter) + (mM) = (millimolar) +} + +CONSTANT { + e0 = 1.60217646e-19 (coulombs) + q10 = 2.7 + + ca = 0.22 (1/ms) + cva = 16 (mV) + cka = -26.5 (mV) + cb = 0.22 (1/ms) + cvb = 16 (mV) + ckb = 26.5 (mV) + + zn = 1.9196 (1) : valence of n-gate +} + +PARAMETER { + gateCurrent = 0 (1) : gating currents ON = 1 OFF = 0 + + gbar = 0.005 (S/cm2) <0,1e9> + gunit = 16 (pS) : unitary conductance +} + +ASSIGNED { + celsius (degC) + v (mV) + + ik (mA/cm2) + igate (mA/cm2) + i (mA/cm2) + + ek (mV) + g (S/cm2) + nc (1/cm2) + qt (1) + + ninf (1) + taun (ms) + alpha (1/ms) + beta (1/ms) +} + +STATE { n } + +INITIAL { + nc = (1e12) * gbar / gunit + qt = q10^((celsius-22 (degC))/10 (degC)) + rateConst(v) + n = ninf +} + +BREAKPOINT { + SOLVE state METHOD cnexp + g = gbar * n^4 + ik = g * (v - ek) + igate = nc * (1e6) * e0 * 4 * zn * ngateFlip() + + if (gateCurrent != 0) { + i = igate + } +} + +DERIVATIVE state { + rateConst(v) + n' = alpha * (1-n) - beta * n +} + +PROCEDURE rateConst(v (mV)) { + alpha = qt * alphaFkt(v) + beta = qt * betaFkt(v) + ninf = alpha / (alpha + beta) + taun = 1 / (alpha + beta) +} + +FUNCTION alphaFkt(v (mV)) (1/ms) { + alphaFkt = ca * exp(-(v+cva)/cka) +} + +FUNCTION betaFkt(v (mV)) (1/ms) { + betaFkt = cb * exp(-(v+cvb)/ckb) +} + +FUNCTION ngateFlip() (1/ms) { + ngateFlip = (ninf-n)/taun +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv34.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv34.mod new file mode 100644 index 00000000..6b187a87 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv34.mod @@ -0,0 +1,100 @@ +: HH TEA-sensitive Purkinje potassium current +: Created 8/5/02 - nwg + +: Suffix from kpkj to Kv3_4 + +NEURON { + SUFFIX Kv3_4 + USEION k READ ek WRITE ik + RANGE gkbar, ik + RANGE minf, hinf, mtau, htau +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) +} + +CONSTANT { + q10 = 3 +} + +PARAMETER { + v (mV) + + gkbar = .004 (mho/cm2) + + mivh = -24 (mV) + mik = 15.4 (1) + mty0 = .00012851 + mtvh1 = 100.7 (mV) + mtk1 = 12.9 (1) + mtvh2 = -56.0 (mV) + mtk2 = -23.1 (1) + + hiy0 = .31 + hiA = .69 + hivh = -5.802 (mV) + hik = 11.2 (1) + + ek +} + +ASSIGNED { + ik (mA/cm2) + minf + mtau (ms) + hinf + htau (ms) + qt +} + +STATE { + m + h +} + +INITIAL { + rates(v) + m = minf + h = hinf + + qt = q10^((celsius-37 (degC))/10 (degC)) +} + +BREAKPOINT { + SOLVE states METHOD cnexp + ik = gkbar * m^3 * h * (v - ek) +} + +DERIVATIVE states { + rates(v) + m' = (minf - m) / mtau + h' = (hinf - h) / htau +} + +PROCEDURE rates( Vm (mV)) { + LOCAL v + v = Vm + 11 : Account for Junction Potential + minf = 1/(1+exp(-(v-mivh)/mik)) + mtau = (1000) * mtau_func(v) /qt + hinf = hiy0 + hiA/(1+exp((v-hivh)/hik)) + htau = 1000 * htau_func(v) / qt +} + +FUNCTION mtau_func (v (mV)) (ms) { + if (v < -35) { + mtau_func = (3.4225e-5+.00498*exp(-v/-28.29))*3 + } else { + mtau_func = (mty0 + 1/(exp((v+mtvh1)/mtk1)+exp((v+mtvh2)/mtk2))) + } +} + +FUNCTION htau_func(Vm (mV)) (ms) { + if ( Vm > 0) { + htau_func = .0012+.0023*exp(-.141*Vm) + } else { + htau_func = 1.2202e-05 + .012 * exp(-((Vm-(-56.3))/49.6)^2) + } +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv3_1.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv3_1.mod new file mode 100644 index 00000000..46f2741b --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv3_1.mod @@ -0,0 +1,55 @@ +:Comment : +:Reference : : Characterization of a Shaw-related potassium channel family in rat brain, The EMBO Journal, vol.11, no.7,2473-2486 (1992) + +NEURON { + SUFFIX Kv3_1 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + mInf = 1/(1+exp(((v -(18.700 + vshift))/(-9.700)))) + mTau = 0.2*20.000/(1+exp(((v -(-46.560 + vshift))/(-44.140)))) + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv43.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv43.mod new file mode 100644 index 00000000..7ef88c14 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Kv43.mod @@ -0,0 +1,154 @@ +TITLE Cerebellum Granule Cell Model + +COMMENT + KA channel + + Author: E.D'Angelo, T.Nieus, A. Fontana + Last revised: Egidio 3.12.2003 + +:Suffix from GRC_KA to Kv4_3 +ENDCOMMENT + +NEURON { + SUFFIX Kv4_3 + USEION k READ ek WRITE ik + RANGE gkbar, ik, g, alpha_a, beta_a, alpha_b, beta_b + RANGE Aalpha_a, Kalpha_a, V0alpha_a + RANGE Abeta_a, Kbeta_a, V0beta_a + RANGE Aalpha_b, Kalpha_b, V0alpha_b + RANGE Abeta_b, Kbeta_b, V0beta_b + RANGE V0_ainf, K_ainf, V0_binf, K_binf + RANGE a_inf, tau_a, b_inf, tau_b +} + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) +} + +PARAMETER { + Aalpha_a = 0.8147 (/ms) :4.88826 + Kalpha_a = -23.32708 (mV) + V0alpha_a = -9.17203 (mV) + Abeta_a = 0.1655 (/ms) : 0.99285 + Kbeta_a = 19.47175 (mV) + V0beta_a = -18.27914 (mV) + + Aalpha_b = 0.0368 (/ms) : 0.11042 + Kalpha_b = 12.8433 (mV) + V0alpha_b = -111.33209 (mV) + Abeta_b = 0.0345(/ms) : 0.10353 + Kbeta_b = -8.90123 (mV) + V0beta_b = -49.9537 (mV) + + V0_ainf = -38(mV) + K_ainf = -17(mV) + + V0_binf = -78.8 (mV) + K_binf = 8.4 (mV) + v (mV) + gkbar= 0.0032 (mho/cm2) :0.003 + celsius = 30 (degC) +} + +STATE { + a + b +} + +ASSIGNED { + ik (mA/cm2) + a_inf + b_inf + tau_a (ms) + tau_b (ms) + g (mho/cm2) + alpha_a (/ms) + beta_a (/ms) + alpha_b (/ms) + beta_b (/ms) + ek (mV) +} + +INITIAL { + rate(v) + a = a_inf + b = b_inf +} + +BREAKPOINT { + SOLVE states METHOD derivimplicit + g = gkbar*a*a*a*b + ik = g*(v - ek) + alpha_a = alp_a(v) + beta_a = bet_a(v) + alpha_b = alp_b(v) + beta_b = bet_b(v) +} + +DERIVATIVE states { + rate(v) + a' =(a_inf - a)/tau_a + b' =(b_inf - b)/tau_b +} + +FUNCTION alp_a(v(mV))(/ms) { LOCAL Q10 + Q10 = 3^((celsius-25.5(degC))/10(degC)) +: alp_a = Q10*Aalpha_a*exp(Kalpha_a*(v-V0alpha_a)) +: alp_a = -0.04148(/mV-ms)*linoid(v+67.697(mV),-3.857(mV)) + alp_a = Q10*Aalpha_a*sigm(v-V0alpha_a,Kalpha_a) +} + +FUNCTION bet_a(v(mV))(/ms) { LOCAL Q10 + Q10 = 3^((celsius-25.5(degC))/10(degC)) +: bet_a = Q10*Abeta_a*exp(Kbeta_a*(v-V0beta_a)) +: bet_a = 0.0359(/mV-ms)*linoid(v+45.878(mV),23.654(mV)) + bet_a = Q10*Abeta_a/(exp((v-V0beta_a)/Kbeta_a)) +} + +FUNCTION alp_b(v(mV))(/ms) { LOCAL Q10 + Q10 = 3^((celsius-25.5(degC))/10(degC)) +: alp_b = Q10*Aalpha_b*exp(Kalpha_b*(v-V0alpha_b)) +: alp_b = 0.356(/mV-ms)*linoid(v+231.03(mV),17.8(mV)) + alp_b = Q10*Aalpha_b*sigm(v-V0alpha_b,Kalpha_b) +} + +FUNCTION bet_b(v(mV))(/ms) { LOCAL Q10 + Q10 = 3^((celsius-25.5(degC))/10(degC)) +: bet_b = Q10*Abeta_b*exp(Kbeta_b*(v-V0beta_b)) +: bet_b = -0.00825(/mV-ms)*linoid(v+43.284(mV),-8.927(mV)) + bet_b = Q10*Abeta_b*sigm(v-V0beta_b,Kbeta_b) +} + +PROCEDURE rate(v (mV)) {LOCAL a_a, b_a, a_b, b_b + TABLE a_inf, tau_a, b_inf, tau_b + DEPEND Aalpha_a, Kalpha_a, V0alpha_a, + Abeta_a, Kbeta_a, V0beta_a, + Aalpha_b, Kalpha_b, V0alpha_b, + Abeta_b, Kbeta_b, V0beta_b, celsius FROM -100 TO 30 WITH 13000 + a_a = alp_a(v) + b_a = bet_a(v) + a_b = alp_b(v) + b_b = bet_b(v) + a_inf = 1/(1+exp((v-V0_ainf)/K_ainf)) + tau_a = 1/(a_a + b_a) + b_inf = 1/(1+exp((v-V0_binf)/K_binf)) + tau_b = 1/(a_b + b_b) +: Bardoni Belluzzi data +: a_inf = 1/(1+exp(-(v+46.7)/19.8)) +: tau_a = 0.41*exp(-(v+43.5)/42.8)+0.167 +: b_inf = 1/(1+exp((v+78.8)/8.4)) +: tau_b = 10.8 + 0.03*v + 1/(57.9*exp(0.127*v)+0.000134*exp(-0.059*v)) +} + +FUNCTION linoid(x (mV),y (mV)) (mV) { + if (fabs(x/y) < 1e-6) { + linoid = y*(1 - x/y/2) + }else{ + linoid = x/(exp(x/y) - 1) + } +} + +FUNCTION sigm(x (mV),y (mV)) { + sigm = 1/(exp(x/y) + 1) +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Leak.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Leak.mod new file mode 100644 index 00000000..4dd56417 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Leak.mod @@ -0,0 +1,77 @@ +COMMENT + + ************************************************** + File generated by: neuroConstruct v1.3.7 + ************************************************** + + +ENDCOMMENT + + +: This is a NEURON mod file generated from a ChannelML file + +: Unit system of original ChannelML file: Physiological Units + +COMMENT + ChannelML file containing a single Channel description +ENDCOMMENT + +TITLE Channel: Leak + +COMMENT + Simple example of a leak/passive conductance. Note: for GENESIS cells with a single leak conductance, + it is better to use the Rm and Em variables for a passive current. +ENDCOMMENT + + +UNITS { + (mA) = (milliamp) + (mV) = (millivolt) + (S) = (siemens) + (um) = (micrometer) + (molar) = (1/liter) + (mM) = (millimolar) + (l) = (liter) +} + + + +NEURON { + + + SUFFIX Leak + : A non specific current is present + RANGE e + NONSPECIFIC_CURRENT il + + RANGE gmax, gion,il + +} + +PARAMETER { + + + gmax = 0.0003 (S/cm2) : default value, should be overwritten when conductance placed on cell + + e = -63 (mV) : default value, should be overwritten when conductance placed on cell + +} + + + +ASSIGNED { + + + v (mV) + + il (mA/cm2) + +} + +BREAKPOINT { + il = gmax*(v - e) + + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTa.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTa.mod new file mode 100755 index 00000000..fcf7bd39 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTa.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTa + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -48 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -69 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTa_t.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTa_t.mod new file mode 100755 index 00000000..44365c9a --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTa_t.mod @@ -0,0 +1,79 @@ +:Reference :Colbert and Pan 2002 + +NEURON { + SUFFIX NaTa_t + USEION na READ ena WRITE ina + RANGE gNaTa_tbar, gNaTa_t, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gNaTa_tbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gNaTa_t (S/cm2) + mInf + mTau (ms) + mAlpha + mBeta + hInf + hTau (ms) + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gNaTa_t = gNaTa_tbar*m*m*m*h + ina = gNaTa_t*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + if(v == -38){ + v = v+0.0001 + } + mAlpha = (0.182 * (v- -38))/(1-(exp(-(v- -38)/6))) + mBeta = (0.124 * (-v -38))/(1-(exp(-(-v -38)/6))) + mTau = (1/(mAlpha + mBeta))/qt + mInf = mAlpha/(mAlpha + mBeta) + + if(v == -66){ + v = v + 0.0001 + } + + hAlpha = (-0.015 * (v- -66))/(1-(exp((v- -66)/6))) + hBeta = (-0.015 * (-v -66))/(1-(exp((-v -66)/6))) + hTau = (1/(hAlpha + hBeta))/qt + hInf = hAlpha/(hAlpha + hBeta) + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTg.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTg.mod new file mode 100644 index 00000000..63051557 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTg.mod @@ -0,0 +1,83 @@ +:Reference :Colbert and Pan 2002 + +NEURON { + SUFFIX NaTg + USEION na READ ena WRITE ina + RANGE gbar, g, ina, vshifth, vshiftm, slopeh, slopem +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshifth = 0 (mV) + vshiftm = 0 (mV) + slopeh = 6 + slopem = 6 +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + if(v == (-38+vshiftm)){ + v = v+0.0001 + } + mAlpha = (0.182 * (v- (-38+vshiftm)))/(1-(exp(-(v- (-38+vshiftm))/slopem))) + mBeta = (0.124 * (-v + (-38+vshiftm)))/(1-(exp(-(-v + (-38+vshiftm))/slopem))) + mTau = (1/(mAlpha + mBeta))/qt + mInf = mAlpha/(mAlpha + mBeta) + + if(v == (-66+vshifth)){ + v = v + 0.0001 + } + + hAlpha = (-0.015 * (v- (-66+vshifth)))/(1-(exp((v- (-66+vshifth))/slopeh))) + hBeta = (-0.015 * (-v +(-66+vshifth)))/(1-(exp((-v +(-66+vshifth))/slopeh))) + hTau = (1/(hAlpha + hBeta))/qt + hInf = hAlpha/(hAlpha + hBeta) + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTs.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTs.mod new file mode 100755 index 00000000..f753e718 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTs.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTs + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -40 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -66 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTs2_t.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTs2_t.mod new file mode 100755 index 00000000..9cdde2b6 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaTs2_t.mod @@ -0,0 +1,79 @@ +:Reference :Colbert and Pan 2002 +:comment: took the NaTa and shifted both activation/inactivation by 6 mv + +NEURON { + SUFFIX NaTs2_t + USEION na READ ena WRITE ina + RANGE gNaTs2_tbar, gNaTs2_t, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gNaTs2_tbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gNaTs2_t (S/cm2) + mInf + mTau (ms) + mAlpha + mBeta + hInf + hTau (ms) + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gNaTs2_t = gNaTs2_tbar*m*m*m*h + ina = gNaTs2_t*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + if(v == -32){ + v = v+0.0001 + } + mAlpha = (0.182 * (v- -32))/(1-(exp(-(v- -32)/6))) + mBeta = (0.124 * (-v -32))/(1-(exp(-(-v -32)/6))) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + if(v == -60){ + v = v + 0.0001 + } + hAlpha = (-0.015 * (v- -60))/(1-(exp((v- -60)/6))) + hBeta = (-0.015 * (-v -60))/(1-(exp((-v -60)/6))) + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaV.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaV.mod new file mode 100755 index 00000000..a7023956 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/NaV.mod @@ -0,0 +1,186 @@ +TITLE Mouse sodium current +: Kinetics of Carter et al. (2012) +: Based on 37 degC recordings from mouse hippocampal CA1 pyramids + +NEURON { + SUFFIX NaV + USEION na READ ena WRITE ina + RANGE g, gbar +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) +} + +PARAMETER { + gbar = .015 (S/cm2) + + : kinetic parameters + Con = 0.01 (/ms) : closed -> inactivated transitions + Coff = 40 (/ms) : inactivated -> closed transitions + Oon = 8 (/ms) : open -> Ineg transition + Ooff = 0.05 (/ms) : Ineg -> open transition + alpha = 400 (/ms) + beta = 12 (/ms) + gamma = 250 (/ms) : opening + delta = 60 (/ms) : closing + + alfac = 2.51 + btfac = 5.32 + + : Vdep + x1 = 24 (mV) : Vdep of activation (alpha) + x2 = -24 (mV) : Vdep of deactivation (beta) +} + +ASSIGNED { + + : rates + f01 (/ms) + f02 (/ms) + f03 (/ms) + f04 (/ms) + f0O (/ms) + f11 (/ms) + f12 (/ms) + f13 (/ms) + f14 (/ms) + f1n (/ms) + fi1 (/ms) + fi2 (/ms) + fi3 (/ms) + fi4 (/ms) + fi5 (/ms) + fin (/ms) + + b01 (/ms) + b02 (/ms) + b03 (/ms) + b04 (/ms) + b0O (/ms) + b11 (/ms) + b12 (/ms) + b13 (/ms) + b14 (/ms) + b1n (/ms) + bi1 (/ms) + bi2 (/ms) + bi3 (/ms) + bi4 (/ms) + bi5 (/ms) + bin (/ms) + + v (mV) + ena (mV) + ina (milliamp/cm2) + g (S/cm2) + celsius (degC) +} + +STATE { + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + C5 FROM 0 TO 1 + I1 FROM 0 TO 1 + I2 FROM 0 TO 1 + I3 FROM 0 TO 1 + I4 FROM 0 TO 1 + I5 FROM 0 TO 1 + O FROM 0 TO 1 + I6 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * O + ina = g * (v - ena) +} + +INITIAL { + rates(v) + SOLVE seqinitial +} + +KINETIC activation +{ + rates(v) + ~ C1 <-> C2 (f01,b01) + ~ C2 <-> C3 (f02,b02) + ~ C3 <-> C4 (f03,b03) + ~ C4 <-> C5 (f04,b04) + ~ C5 <-> O (f0O,b0O) + ~ O <-> I6 (fin,bin) + ~ I1 <-> I2 (f11,b11) + ~ I2 <-> I3 (f12,b12) + ~ I3 <-> I4 (f13,b13) + ~ I4 <-> I5 (f14,b14) + ~ I5 <-> I6 (f1n,b1n) + ~ C1 <-> I1 (fi1,bi1) + ~ C2 <-> I2 (fi2,bi2) + ~ C3 <-> I3 (fi3,bi3) + ~ C4 <-> I4 (fi4,bi4) + ~ C5 <-> I5 (fi5,bi5) + + CONSERVE C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +LINEAR seqinitial { : sets initial equilibrium + ~ I1*bi1 + C2*b01 - C1*( fi1+f01) = 0 + ~ C1*f01 + I2*bi2 + C3*b02 - C2*(b01+fi2+f02) = 0 + ~ C2*f02 + I3*bi3 + C4*b03 - C3*(b02+fi3+f03) = 0 + ~ C3*f03 + I4*bi4 + C5*b04 - C4*(b03+fi4+f04) = 0 + ~ C4*f04 + I5*bi5 + O*b0O - C5*(b04+fi5+f0O) = 0 + ~ C5*f0O + I6*bin - O*(b0O+fin) = 0 + + ~ C1*fi1 + I2*b11 - I1*( bi1+f11) = 0 + ~ I1*f11 + C2*fi2 + I3*b12 - I2*(b11+bi2+f12) = 0 + ~ I2*f12 + C3*fi3 + I4*bi3 - I3*(b12+bi3+f13) = 0 + ~ I3*f13 + C4*fi4 + I5*b14 - I4*(b13+bi4+f14) = 0 + ~ I4*f14 + C5*fi5 + I6*b1n - I5*(b14+bi5+f1n) = 0 + + ~ C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +PROCEDURE rates(v(mV) ) +{ + LOCAL qt + qt = 2.3^((celsius-37)/10) + + f01 = qt * 4 * alpha * exp(v/x1) + f02 = qt * 3 * alpha * exp(v/x1) + f03 = qt * 2 * alpha * exp(v/x1) + f04 = qt * 1 * alpha * exp(v/x1) + f0O = qt * gamma + f11 = qt * 4 * alpha * alfac * exp(v/x1) + f12 = qt * 3 * alpha * alfac * exp(v/x1) + f13 = qt * 2 * alpha * alfac * exp(v/x1) + f14 = qt * 1 * alpha * alfac * exp(v/x1) + f1n = qt * gamma + fi1 = qt * Con + fi2 = qt * Con * alfac + fi3 = qt * Con * alfac^2 + fi4 = qt * Con * alfac^3 + fi5 = qt * Con * alfac^4 + fin = qt * Oon + + b01 = qt * 1 * beta * exp(v/x2) + b02 = qt * 2 * beta * exp(v/x2) + b03 = qt * 3 * beta * exp(v/x2) + b04 = qt * 4 * beta * exp(v/x2) + b0O = qt * delta + b11 = qt * 1 * beta * exp(v/x2) / btfac + b12 = qt * 2 * beta * exp(v/x2) / btfac + b13 = qt * 3 * beta * exp(v/x2) / btfac + b14 = qt * 4 * beta * exp(v/x2) / btfac + b1n = qt * delta + bi1 = qt * Coff + bi2 = qt * Coff / (btfac) + bi3 = qt * Coff / (btfac^2) + bi4 = qt * Coff / (btfac^3) + bi5 = qt * Coff / (btfac^4) + bin = qt * Ooff +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nap.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nap.mod new file mode 100644 index 00000000..245fce35 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nap.mod @@ -0,0 +1,85 @@ +:Comment : mtau deduced from text (said to be 6 times faster than for NaTa) +:Comment : so I used the equations from NaT and multiplied by 6 +:Reference : Modeled according to kinetics derived from Magistretti & Alonso 1999 +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Nap + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + mInf = 1.0/(1+exp((v- -52.6)/-4.6)) + if(v == -38){ + v = v+0.0001 + } + mAlpha = (0.182 * (v- -38))/(1-(exp(-(v- -38)/6))) + mBeta = (0.124 * (-v -38))/(1-(exp(-(-v -38)/6))) + mTau = 6*(1/(mAlpha + mBeta))/qt + + if(v == -17){ + v = v + 0.0001 + } + if(v == -64.4){ + v = v+0.0001 + } + + hInf = 1.0/(1+exp((v- -48.8)/10)) + hAlpha = -2.88e-6 * (v + 17) / (1 - exp((v + 17)/4.63)) + hBeta = 6.94e-6 * (v + 64.4) / (1 - exp(-(v + 64.4)/2.63)) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nap_Et2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nap_Et2.mod new file mode 100755 index 00000000..c0e52bb6 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nap_Et2.mod @@ -0,0 +1,85 @@ +:Comment : mtau deduced from text (said to be 6 times faster than for NaTa) +:Comment : so I used the equations from NaT and multiplied by 6 +:Reference : Modeled according to kinetics derived from Magistretti & Alonso 1999 +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Nap_Et2 + USEION na READ ena WRITE ina + RANGE gNap_Et2bar, gNap_Et2, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gNap_Et2bar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gNap_Et2 (S/cm2) + mInf + mTau (ms) + mAlpha + mBeta + hInf + hTau (ms) + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gNap_Et2 = gNap_Et2bar*m*m*m*h + ina = gNap_Et2*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + mInf = 1.0/(1+exp((v- -52.6)/-4.6)) + if(v == -38){ + v = v+0.0001 + } + mAlpha = (0.182 * (v- -38))/(1-(exp(-(v- -38)/6))) + mBeta = (0.124 * (-v -38))/(1-(exp(-(-v -38)/6))) + mTau = 6*(1/(mAlpha + mBeta))/qt + + if(v == -17){ + v = v + 0.0001 + } + if(v == -64.4){ + v = v+0.0001 + } + + hInf = 1.0/(1+exp((v- -48.8)/10)) + hAlpha = -2.88e-6 * (v + 17) / (1 - exp((v + 17)/4.63)) + hBeta = 6.94e-6 * (v + 64.4) / (1 - exp(-(v + 64.4)/2.63)) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nav16.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nav16.mod new file mode 100644 index 00000000..2fa601d1 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/Nav16.mod @@ -0,0 +1,203 @@ +TITLE resurgent sodium channel + +COMMENT +Neuron implementation of a resurgent sodium channel (with blocking particle) +Based om updated kinetic parameters from Raman and Bean, Biophys.J. 80 (2001) 729 + +Modified from Khaliq et al., J.Neurosci. 23(2003)4899 +by qt-correction of all rate constants + +Laboratory for Neuronal Circuit Dynamics +RIKEN Brain Science Institute, Wako City, Japan +http://www.neurodynamics.brain.riken.jp + +Reference: Akemann and Knoepfel, J.Neurosci. 26 (2006) 4602 +Date of Implementation: May 2005 +Contact: akemann@brain.riken.jp + +Suffix from Narsg to Nav1_6 + +ENDCOMMENT + +NEURON { + SUFFIX Nav1_6 + USEION na READ ena WRITE ina + RANGE g, gbar, ina, f0O, fin, fip + +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) +} + +CONSTANT { + q10 = 3 +} + +PARAMETER { + gbar = 0.016 (S/cm2) + celsius (degC) + + : kinetic parameters + Con = 0.005 (/ms) : closed -> inactivated transitions + Coff = 0.5 (/ms) : inactivated -> closed transitions + Oon = 0.75 (/ms) : open -> Ineg transition + Ooff = 0.005 (/ms) : Ineg -> open transition + alpha = 150 (/ms) : activation + beta = 3 (/ms) : deactivation + gamma = 150 (/ms) : opening + delta = 40 (/ms) : closing, greater than BEAN/KUO = 0.2 + epsilon = 1.75 (/ms) : open -> Iplus for tau = 0.3 ms at +30 with x5 + zeta = 0.03 (/ms) : Iplus -> open for tau = 25 ms at -30 with x6 + + : Vdep + x1 = 20 (mV) : Vdep of activation (alpha) + x2 = -20 (mV) : Vdep of deactivation (beta) + x3 = 1e12 (mV) : Vdep of opening (gamma) + x4 = -1e12 (mV) : Vdep of closing (delta) + x5 = 1e12 (mV) : Vdep into Ipos (epsilon) + x6 = -25 (mV) : Vdep out of Ipos (zeta) +} + +ASSIGNED { + alfac : microscopic reversibility factors + btfac + + : rates + f01 (/ms) + f02 (/ms) + f03 (/ms) + f04 (/ms) + f0O (/ms) + fip (/ms) + f11 (/ms) + f12 (/ms) + f13 (/ms) + f14 (/ms) + f1n (/ms) + fi1 (/ms) + fi2 (/ms) + fi3 (/ms) + fi4 (/ms) + fi5 (/ms) + fin (/ms) + + b01 (/ms) + b02 (/ms) + b03 (/ms) + b04 (/ms) + b0O (/ms) + bip (/ms) + b11 (/ms) + b12 (/ms) + b13 (/ms) + b14 (/ms) + b1n (/ms) + bi1 (/ms) + bi2 (/ms) + bi3 (/ms) + bi4 (/ms) + bi5 (/ms) + bin (/ms) + + v (mV) + ena (mV) + ina (milliamp/cm2) + g (S/cm2) + qt +} + +STATE { + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + C5 FROM 0 TO 1 + I1 FROM 0 TO 1 + I2 FROM 0 TO 1 + I3 FROM 0 TO 1 + I4 FROM 0 TO 1 + I5 FROM 0 TO 1 + O FROM 0 TO 1 + B FROM 0 TO 1 + I6 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * O + ina = g * (v - ena) +} + +INITIAL { + qt = q10^((celsius-22 (degC))/10 (degC)) + rates(v) +} + +KINETIC activation +{ + rates(v) + ~ C1 <-> C2 (f01,b01) + ~ C2 <-> C3 (f02,b02) + ~ C3 <-> C4 (f03,b03) + ~ C4 <-> C5 (f04,b04) + ~ C5 <-> O (f0O,b0O) + ~ O <-> B (fip,bip) + ~ O <-> I6 (fin,bin) + ~ I1 <-> I2 (f11,b11) + ~ I2 <-> I3 (f12,b12) + ~ I3 <-> I4 (f13,b13) + ~ I4 <-> I5 (f14,b14) + ~ I5 <-> I6 (f1n,b1n) + ~ C1 <-> I1 (fi1,bi1) + ~ C2 <-> I2 (fi2,bi2) + ~ C3 <-> I3 (fi3,bi3) + ~ C4 <-> I4 (fi4,bi4) + ~ C5 <-> I5 (fi5,bi5) + +CONSERVE C1 + C2 + C3 + C4 + C5 + O + B + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + + +PROCEDURE rates(v(mV) ) +{ + alfac = (Oon/Con)^(1/4) + btfac = (Ooff/Coff)^(1/4) + f01 = 4 * alpha * exp(v/x1) * qt + f02 = 3 * alpha * exp(v/x1) * qt + f03 = 2 * alpha * exp(v/x1) * qt + f04 = 1 * alpha * exp(v/x1) * qt + f0O = gamma * exp(v/x3) * qt + fip = epsilon * exp(v/x5) * qt + f11 = 4 * alpha * alfac * exp(v/x1) * qt + f12 = 3 * alpha * alfac * exp(v/x1) * qt + f13 = 2 * alpha * alfac * exp(v/x1) * qt + f14 = 1 * alpha * alfac * exp(v/x1) * qt + f1n = gamma * exp(v/x3) * qt + fi1 = Con * qt + fi2 = Con * alfac * qt + fi3 = Con * alfac^2 * qt + fi4 = Con * alfac^3 * qt + fi5 = Con * alfac^4 * qt + fin = Oon * qt + + b01 = 1 * beta * exp(v/x2) * qt + b02 = 2 * beta * exp(v/x2) * qt + b03 = 3 * beta * exp(v/x2) * qt + b04 = 4 * beta * exp(v/x2) * qt + b0O = delta * exp(v/x4) * qt + bip = zeta * exp(v/x6) * qt + b11 = 1 * beta * btfac * exp(v/x2) * qt + b12 = 2 * beta * btfac * exp(v/x2) * qt + b13 = 3 * beta * btfac * exp(v/x2) * qt + b14 = 4 * beta * btfac * exp(v/x2) * qt + b1n = delta * exp(v/x4) * qt + bi1 = Coff * qt + bi2 = Coff * btfac * qt + bi3 = Coff * btfac^2 * qt + bi4 = Coff * btfac^3 * qt + bi5 = Coff * btfac^4 * qt + bin = Ooff * qt +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/PC_Gaba_det_vi_alfa1.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/PC_Gaba_det_vi_alfa1.mod new file mode 100644 index 00000000..9a413049 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/PC_Gaba_det_vi_alfa1.mod @@ -0,0 +1,307 @@ +TITLE Gaba mod file only for fitting purpose + +COMMENT +Thierry Nieus, unpublished results, please keep reserved. +Variables with suffix "_s" are meant for single IPSC fit, with suffix "_2" for CGP experimental condition + Does not work with variable dt! +ENDCOMMENT + + + +NEURON { + POINT_PROCESS PC_gaba_alpha1 + + NONSPECIFIC_CURRENT i + RANGE Q10_diff,Q10_channel + RANGE g, ic ,Cdur ,Tmax, Erev + RANGE Open + + RANGE kon,koff,d3,r3,d1d2,r1r2,a1,b1,a2,b2,r1,r2,d1,d2 + + RANGE gmaxA1,onSET + RANGE gA1 + + RANGE xout,yout,zout,uout + + RANGE tau_1,tau_rec,tau_facil,U,T,U_2 + RANGE diff_flag,diff_flag2,M,Rd,Diff,lamd + RANGE nd,diffus ,Trelease, gmax_factor, syntype + RANGE C, CA1, CA2, DA1, DA2, DA2f, OA1, OA2 +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) + (mM) = (milli/liter) + (pS) = (picosiemens) + PI = (pi)(1) + } + + + + PARAMETER { + syntype + gmax_factor = 1 + : Parametri Postsinaptici + gmaxA1 = 918.807 (pS) + Q10_diff = 1.5 + Q10_channel = 2.4 + Cdur = 0.3 (ms) + + : A1 kinetic rates EC50=(around) 20 uM, U=0.35 + kon = 20 (/ms/mM) : 20 + koff = 2 (/ms) + d3 = 15 (/ms) : 15 + r3 = 0.15 (/ms) : 0.15, use 3.75 for slices in the PR model + d1d2 = 15 (/ms/mM) : 15 + r1r2 = 0.007 (/ms) : 0.007 + a1 = 0.06 (/ms) : 0.06 + b1 = 0.03 (/ms) + a2 = 0.4 (/ms) : 0.4 + b2 = 10 (/ms) + r1 = 7e-4 (/ms) + r2 = 6e-3 (/ms) : 6e-3 + d1 = 3.3e-4 (/ms) + d2 = 1.2 (/ms) : 1.2 + + Erev = -60 (mV) + + : Parametri Presinaptici + tau_1 = 0.1 (ms) < 1e-9, 1e9 > + tau_rec = 36.169 (ms) < 1e-9, 1e9 > :43.4 + tau_facil = 58.517 (ms) < 0, 1e9 > : 6.22 + U = 0.35 < 0, 1 > + + + Tmax = 1 (mM) + onSET = 1 + + + : Diffusion parameters + : Diffusion: M=21.500, R=1.033, D=0.223, lamd=0.02 as in excitatory synapses + + M = 7.506 : numero di (kilo) molecole in una vescicola (52.76) + Rd = 0.978 (um) :4.79 :4.96 : 4.96 (first fit!) + Diff = 0.223 (um2/ms) + + lamd = 20 (nm) + diff_flag = 1 : flag diffusion on/off + + nd = 1 : kernel exponent of diffusion + + + celsius (degC) +} + + +ASSIGNED { + v (mV) : postsynaptic voltage + i (nA) : current = g*(v - Erev) + ic (nA) : current = g*(v - Erev) + g (pS) : conductance + gA1 (nA) + + Open + diffus + + T (mM) + + Trelease (mM) + + Mres (mM) + tpre (ms) + + xout + yout + zout + uout + + tspike[100] (ms) : will be initialized by the pointprocess + PRE[100] + :PRE_2[500] + numpulses + tzero + gbar_Q10 (mho/cm2) + Q10 (1) +} + +STATE { + : CTRL - A1 + C + CA1 + CA2 + DA1 + DA2 + DA2f + OA1 + OA2 + +} + +INITIAL { + : CTRL - A1 + C=1 + CA1=0 + CA2=0 + DA1=0 + DA2=0 + DA2f=0 + OA1=0 + OA2=0 + + + Open=0 + + T=0 (mM) + + gbar_Q10 = Q10_diff^((celsius-30)/10) + Q10 = Q10_channel^((celsius-30)/10) + + numpulses=0 + Mres=1e3* (1e3 * 1e15 / 6.022e23 * M) : (M) to (mM) so 1e3, 1um^3=1dm^3*1e-15 so 1e15 + FROM i=1 TO 100 { PRE[i-1]=0 tspike[i-1]=0 } :PRE_2[500]=0} + tspike[0]=1e12 (ms) + if(tau_1>=tau_rec){ + printf("Warning: tau_1 (%g) should never be higher neither equal to tau_rec (%g)!\n",tau_1,tau_rec) + tau_rec=tau_1+1e-5 + :printf("tau_rec has been set to %g\n",tau_rec) + } + onSET = 1 + +} + FUNCTION imax(a,b) { + if (a>b) { imax=a } + else { imax=b } + } + +FUNCTION diffusione(){ + LOCAL DifWave,i,cntc,fi,aaa + DifWave=0 + cntc=imax(numpulses-100,0) + FROM i=cntc TO numpulses{ + fi=fmod(i,100) + tzero=tspike[fi] + if(t>tzero){ + aaa = (-Rd*Rd/(4*Diff*(t-tzero))) + if(fabs(aaa)<699){ + DifWave=DifWave+PRE[fi]*Mres*exp(aaa)/((4*PI*Diff*(1e-3)*lamd)*(t-tzero)) : ^nd nd =1 + }else{ + if(aaa>0){ + DifWave=DifWave+PRE[fi]*Mres*exp(699)/((4*PI*Diff*(1e-3)*lamd)*(t-tzero)) : ^nd nd =1 + }else{ + DifWave=DifWave+PRE[fi]*Mres*exp(-699)/((4*PI*Diff*(1e-3)*lamd)*(t-tzero)) : ^nd nd =1 + } + } + } + } + diffusione=DifWave +} + + + +BREAKPOINT { + SOLVE kstates METHOD sparse + + Open=OA1+OA2 + + gA1 = gmaxA1 * gbar_Q10 * Open + + g = gA1 * gmax_factor + + i = (1e-6) * g * ( v - Erev ) + ic = i +} + +KINETIC kstates { + + : ************ + : *** CTRL *** + : ************ + + + diffus=diffusione() + Trelease = T :+ diffus + + : *** ALFA 1 *** + + : second row + ~ C <-> CA1 (2*kon*Trelease*Q10,koff*Q10) + ~ CA1 <-> CA2 (kon*Trelease*Q10,2*koff*Q10) + ~ CA2 <-> DA2f (d3*Q10,r3*Q10) + : third row + ~ DA1 <-> DA2 (d1d2*Trelease*Q10,r1r2*Q10) + : first <=> second row + ~ OA1 <-> CA1 (a1*Q10,b1*Q10) + ~ OA2 <-> CA2 (a2*Q10,b2*Q10) + : third <=> second row + ~ DA1 <-> CA1 (r1*Q10,d1*Q10) + ~ DA2 <-> CA2 (r2*Q10,d2*Q10) + CONSERVE C+CA1+CA2+DA1+DA2+DA2f+OA1+OA2=1 + +} + + +NET_RECEIVE(weight,on, nspike,tzero (ms),x,y,z,u, tsyn (ms)) {LOCAL fi + INITIAL { + x = 0 + y = 0 + z = 0 + u = 0 + + xout=x + yout=y + zout=z + uout=u + tsyn = t + nspike = 1 + } + + if(onSET){ + on=0 + onSET=0 + + } + + if (flag == 0) { + : Qui faccio rientrare la modulazione presinaptica + nspike = nspike + 1 + :printf("T = %g (mM)\n",Trelease) + if (!on) { + tzero = t + tpre=t : activates diffusion + on = 1 + + z = z*exp(-(t - tsyn)/tau_rec) + z = z + ( y*(exp(-(t - tsyn)/tau_1) - exp(-(t - tsyn)/tau_rec)) / ((tau_1/tau_rec)-1) ) + y = y*exp(-(t - tsyn)/tau_1) + x = 1-y-z + if (tau_facil > 0) { + u = u*exp(-(t - tsyn)/tau_facil) + u = u + U * ( 1 - u ) + } else { u = U } + y = y + x * u + T=Tmax*y + + + + fi=fmod(numpulses,100) + PRE[fi]=y : PRE[numpulses]=y + + tspike[fi] = t + numpulses=numpulses+1 + + + + tsyn = t + } + net_send(Cdur, nspike) + + } + + if (flag == nspike) { + T = 0 + + on = 0 + } +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/PURKINJE_Ampa_det_vi.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/PURKINJE_Ampa_det_vi.mod new file mode 100644 index 00000000..ff3ba55e --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/PURKINJE_Ampa_det_vi.mod @@ -0,0 +1,234 @@ +TITLE + +COMMENT +ENDCOMMENT + +NEURON { + POINT_PROCESS PF_syn + NONSPECIFIC_CURRENT i + RANGE Q10_diff,Q10_channel + RANGE R, g, ic + RANGE Cdur, Erev + RANGE r1FIX, r2, r3,r4,r5,gmax,r1,r6,r6FIX,kB + RANGE tau_1, tau_rec, tau_facil, U + RANGE PRE,T,Tmax,x + RANGE y_read,z_read,u_read + RANGE C,O,D + + RANGE diffuse,Trelease,lamd + RANGE M,Diff,Rd + + RANGE tspike + RANGE nd, syntype, gmax_factor +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) + (mM) = (milli/liter) + (pS) = (picosiemens) + (nS) = (nanosiemens) + (um) = (micrometer) + PI = (pi) (1) + } + + PARAMETER { + syntype + gmax_factor = 1 + Q10_diff = 1.1 + Q10_channel = 2.4 + : Parametri Postsinaptici + gmax = 1200 (pS) + + U = 0.13 (1) < 0, 1 > + tau_rec = 35.1 (ms) < 1e-9, 1e9 > + tau_facil = 54 (ms) < 0, 1e9 > + + M = 21.515 : numero di (kilo) molecole in una vescicola + Rd = 1.03 (um) + Diff = 0.223 (um2/ms) + + Cdur = 0.3 (ms) + r1FIX = 5.4 : 1.66 (/ms/mM) + r2 = 0.82 : 0.137 (/ms) + r3 = 0 (/ms) + r4 = 0 (/ms) + r5 = 0.013 : 0.013 (/ms) + r6FIX = 1.12 : 0.237 (/ms/mM) + Erev = 0 (mV) + kB = 0.44 (mM) + + : Parametri Presinaptici + tau_1 = 6 (ms) < 1e-9, 1e9 > + + + + u0 = 0 (1) < 0, 1 > : se u0=0 al primo colpo y=U + Tmax = 1 (mM) + + : Diffusion + diffuse = 0 + + lamd = 20 (nm) + nd = 1 + celsius (degC) +} + + +ASSIGNED { + v (mV) : postsynaptic voltage + i (nA) : current = g*(v - Erev) + ic (nA) : current = g*(v - Erev) + g (pS) : conductance + r1 (/ms) + r6 (/ms) + T (mM) + + Trelease (mM) + tspike[800] (ms) + x + y_read + z_read + u_read + tsyn (ms) + PRE[800] + + Mres (mM) + numpulses + tzero + gbar_Q10 (mho/cm2) + Q10 (1) +} + +STATE { + C + O + D +} + +INITIAL { + C=1 + O=0 + D=0 + T=0 (mM) + numpulses=0 + Trelease=0 (mM) + tspike[0]=1e12 (ms) + + gbar_Q10 = Q10_diff^((celsius-30)/10) + Q10 = Q10_channel^((celsius-30)/10) + + : fattore di conversione che comprende molecole -> mM + : n molecole/(Na*V) -> M/(6.022e23*1dm^3) + + Mres = 1e3 * ( 1e3 * 1e15 / 6.022e23 * M ) : (M) to (mM) so 1e3, 1um^3=1dm^3*1e-15 so 1e15 + numpulses=0 + + FROM i=1 TO 800 { PRE[i-1]=0 tspike[i-1]=0 } + tspike[0]=1e12 (ms) + if(tau_1>=tau_rec){ + printf("Warning: tau_1 (%g) should never be higher neither equal to tau_rec (%g)!\n",tau_1,tau_rec) + tau_rec=tau_1+1e-5 + } + } + + FUNCTION imax(a,b) { + if (a>b) { imax=a } + else { imax=b } + } + +FUNCTION diffusione(){ + LOCAL DifWave,i,cntc,fi + DifWave=0 + cntc=imax(numpulses-5,0) + FROM i=cntc TO numpulses{ + :printf ("%g %g ",numpulses,fmod(numpulses,10)) + fi=fmod(i,800) + :printf ("%g %g %g __ ",i,numpulses,fi) + tzero=tspike[fi] + if(t>tzero){ + DifWave=DifWave+PRE[fi]*Mres*exp(-Rd*Rd/(4*Diff*(t-tzero)))/((4*PI*Diff*(1e-3)*lamd)*(t-tzero))^nd + } + } + :printf("\n\n") + diffusione=DifWave +} + +BREAKPOINT { + + if ( diffuse && (t>tspike[0]) ) { Trelease= T + diffusione() } else { Trelease=T } + + SOLVE kstates METHOD sparse + g = gmax * gbar_Q10 * O + i = (1e-6) * g * (v - Erev) * gmax_factor + ic = i +} + +KINETIC kstates { + r1 = r1FIX * Trelease^2 / (Trelease + kB)^2 + r6 = r6FIX * Trelease^2 / (Trelease + kB)^2 + ~ C <-> O (r1*Q10,r2*Q10) + :~ O <-> D (r3*Q10,r4*Q10) + ~ D <-> C (r5*Q10,r6*Q10) + CONSERVE C+O+D = 1 +} + + +NET_RECEIVE(weight, on, nspike, tzero (ms),y, z, u, tsyn (ms)) {LOCAL fi + +: *********** ATTENZIONE! *********** +: +: Qualora si vogliano utilizzare impulsi di glutammato saturanti e' +: necessario che il pulse sia piu' corto dell'intera simulazione +: altrimenti la variabile on non torna al suo valore di default. + +INITIAL { + y = 0 + z = 0 + u = u0 + tsyn = t + nspike = 1 +} + if (flag == 0) { + : Qui faccio rientrare la modulazione presinaptica + nspike = nspike + 1 + if (!on) { + tzero = t + on = 1 + z = z*exp( - (t - tsyn) / (tau_rec) ) + z = z + ( y*(exp(-(t - tsyn)/tau_1) - exp(-(t - tsyn)/(tau_rec)))/((tau_1/(tau_rec))-1) ) + y = y*exp(-(t - tsyn)/tau_1) + x = 1-y-z + + if (tau_facil > 0) { + u = u*exp(-(t - tsyn)/tau_facil) + u = u + U * (1+30*u) * (exp(-5*u)- exp(-5)) + } else { u = U } + + y = y + x * u + + T=Tmax*y + fi=fmod(numpulses,800) + PRE[fi]=y : PRE[numpulses]=y + y_read = y + z_read = z + u_read = u + :PRE=1 : Istruzione non necessaria ma se ommesso allora le copie dell'oggetto successive alla prima non funzionano! + :} + : all'inizio numpulses=0 ! + + tspike[fi] = t + numpulses=numpulses+1 + tsyn = t + + } + net_send(Cdur, nspike) + } + if (flag == nspike) { + tzero = t + T = 0 + on = 0 + } +} + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/ProbAMPANMDA2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/ProbAMPANMDA2.mod new file mode 100644 index 00000000..8d88b75c --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/ProbAMPANMDA2.mod @@ -0,0 +1,204 @@ +TITLE AMPA and NMDA receptor with presynaptic short-term plasticity + + +COMMENT +AMPA and NMDA receptor conductance using a dual-exponential profile +presynaptic short-term plasticity based on Fuhrmann et al. 2002 +Implemented by Srikanth Ramaswamy, Blue Brain Project, July 2009 +Etay: changed weight to be equal for NMDA and AMPA, gmax accessible in Neuron + +ENDCOMMENT + + +NEURON { + + POINT_PROCESS ProbAMPANMDA2 + RANGE tau_r_AMPA, tau_d_AMPA, tau_r_NMDA, tau_d_NMDA + RANGE Use, u, Dep, Fac, u0, weight_NMDA + RANGE i, i_AMPA, i_NMDA, g_AMPA, g_NMDA, e, gmax + NONSPECIFIC_CURRENT i, i_AMPA,i_NMDA + POINTER rng +} + +PARAMETER { + + tau_r_AMPA = 0.2 (ms) : dual-exponential conductance profile + tau_d_AMPA = 1.7 (ms) : IMPORTANT: tau_r < tau_d + tau_r_NMDA = 0.29 (ms) : dual-exponential conductance profile + tau_d_NMDA = 43 (ms) : IMPORTANT: tau_r < tau_d + Use = 1.0 (1) : Utilization of synaptic efficacy (just initial values! Use, Dep and Fac are overwritten by BlueBuilder assigned values) + Dep = 100 (ms) : relaxation time constant from depression + Fac = 10 (ms) : relaxation time constant from facilitation + e = 0 (mV) : AMPA and NMDA reversal potential + mg = 1 (mM) : initial concentration of mg2+ + mggate + gmax = .001 (uS) : weight conversion factor (from nS to uS) + u0 = 0 :initial value of u, which is the running value of Use +} + +COMMENT +The Verbatim block is needed to generate random nos. from a uniform distribution between 0 and 1 +for comparison with Pr to decide whether to activate the synapse or not +ENDCOMMENT + +VERBATIM + +#include +#include +#include + +double nrn_random_pick(void* r); +void* nrn_random_arg(int argpos); + +ENDVERBATIM + + +ASSIGNED { + + v (mV) + i (nA) + i_AMPA (nA) + i_NMDA (nA) + g_AMPA (uS) + g_NMDA (uS) + factor_AMPA + factor_NMDA + rng + weight_NMDA +} + +STATE { + + A_AMPA : AMPA state variable to construct the dual-exponential profile - decays with conductance tau_r_AMPA + B_AMPA : AMPA state variable to construct the dual-exponential profile - decays with conductance tau_d_AMPA + A_NMDA : NMDA state variable to construct the dual-exponential profile - decays with conductance tau_r_NMDA + B_NMDA : NMDA state variable to construct the dual-exponential profile - decays with conductance tau_d_NMDA +} + +INITIAL{ + + LOCAL tp_AMPA, tp_NMDA + + A_AMPA = 0 + B_AMPA = 0 + + A_NMDA = 0 + B_NMDA = 0 + + tp_AMPA = (tau_r_AMPA*tau_d_AMPA)/(tau_d_AMPA-tau_r_AMPA)*log(tau_d_AMPA/tau_r_AMPA) :time to peak of the conductance + tp_NMDA = (tau_r_NMDA*tau_d_NMDA)/(tau_d_NMDA-tau_r_NMDA)*log(tau_d_NMDA/tau_r_NMDA) :time to peak of the conductance + + factor_AMPA = -exp(-tp_AMPA/tau_r_AMPA)+exp(-tp_AMPA/tau_d_AMPA) :AMPA Normalization factor - so that when t = tp_AMPA, gsyn = gpeak + factor_AMPA = 1/factor_AMPA + + factor_NMDA = -exp(-tp_NMDA/tau_r_NMDA)+exp(-tp_NMDA/tau_d_NMDA) :NMDA Normalization factor - so that when t = tp_NMDA, gsyn = gpeak + factor_NMDA = 1/factor_NMDA + +} + +BREAKPOINT { + + SOLVE state METHOD cnexp + mggate = 1 / (1 + exp(0.062 (/mV) * -(v)) * (mg / 3.57 (mM))) :mggate kinetics - Jahr & Stevens 1990 + g_AMPA = gmax*(B_AMPA-A_AMPA) :compute time varying conductance as the difference of state variables B_AMPA and A_AMPA + g_NMDA = gmax*(B_NMDA-A_NMDA) * mggate :compute time varying conductance as the difference of state variables B_NMDA and A_NMDA and mggate kinetics + i_AMPA = g_AMPA*(v-e) :compute the AMPA driving force based on the time varying conductance, membrane potential, and AMPA reversal + i_NMDA = g_NMDA*(v-e) :compute the NMDA driving force based on the time varying conductance, membrane potential, and NMDA reversal + i = i_AMPA + i_NMDA +} + +DERIVATIVE state{ + + A_AMPA' = -A_AMPA/tau_r_AMPA + B_AMPA' = -B_AMPA/tau_d_AMPA + A_NMDA' = -A_NMDA/tau_r_NMDA + B_NMDA' = -B_NMDA/tau_d_NMDA +} + + +NET_RECEIVE (weight,weight_AMPA, weight_NMDA, Pv, Pr, u, tsyn (ms)){ + + weight_AMPA = weight + weight_NMDA = weight + :printf("NMDA weight = %g\n", weight_NMDA) + + INITIAL{ + Pv=1 + u=u0 + tsyn=t + } + + : calc u at event- + if (Fac > 0) { + u = u*exp(-(t - tsyn)/Fac) :update facilitation variable if Fac>0 Eq. 2 in Fuhrmann et al. + } else { + u = Use + } + if(Fac > 0){ + u = u + Use*(1-u) :update facilitation variable if Fac>0 Eq. 2 in Fuhrmann et al. + } + + + Pv = 1 - (1-Pv) * exp(-(t-tsyn)/Dep) :Probability Pv for a vesicle to be available for release, analogous to the pool of synaptic + :resources available for release in the deterministic model. Eq. 3 in Fuhrmann et al. + Pr = u * Pv :Pr is calculated as Pv * u (running value of Use) + Pv = Pv - u * Pv :update Pv as per Eq. 3 in Fuhrmann et al. + :printf("Pv = %g\n", Pv) + :printf("Pr = %g\n", Pr) + tsyn = t + + if (erand() < Pr){ + + A_AMPA = A_AMPA + weight_AMPA*factor_AMPA + B_AMPA = B_AMPA + weight_AMPA*factor_AMPA + A_NMDA = A_NMDA + weight_NMDA*factor_NMDA + B_NMDA = B_NMDA + weight_NMDA*factor_NMDA + + } +} + +PROCEDURE setRNG() { +VERBATIM + { + /** + * This function takes a NEURON Random object declared in hoc and makes it usable by this mod file. + * Note that this method is taken from Brett paper as used by netstim.hoc and netstim.mod + * which points out that the Random must be in negexp(1) mode + */ + void** pv = (void**)(&_p_rng); + if( ifarg(1)) { + *pv = nrn_random_arg(1); + } else { + *pv = (void*)0; + } + } +ENDVERBATIM +} + +FUNCTION erand() { +VERBATIM + //FILE *fi; + double value; + if (_p_rng) { + /* + :Supports separate independent but reproducible streams for + : each instance. However, the corresponding hoc Random + : distribution MUST be set to Random.negexp(1) + */ + value = nrn_random_pick(_p_rng); + //fi = fopen("RandomStreamMCellRan4.txt", "w"); + //fprintf(fi,"random stream for this simulation = %lf\n",value); + //printf("random stream for this simulation = %lf\n",value); + return value; + }else{ +ENDVERBATIM + : the old standby. Cannot use if reproducible parallel sim + : independent of nhost or which host this instance is on + : is desired, since each instance on this cpu draws from + : the same stream + erand = exprand(1) +VERBATIM + } +ENDVERBATIM + erand = value +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/ProbUDFsyn2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/ProbUDFsyn2.mod new file mode 100644 index 00000000..9e152cfa --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/ProbUDFsyn2.mod @@ -0,0 +1,174 @@ +TITLE AMPA and NMDA receptor with presynaptic short-term plasticity + + +COMMENT +AMPA and NMDA receptor conductance using a dual-exponential profile +presynaptic short-term plasticity based on Fuhrmann et al. 2002 +Implemented by Srikanth Ramaswamy, Blue Brain Project, July 2009 +Etay: changed weight to be equal for NMDA and AMPA, gmax accessible in Neuron + +ENDCOMMENT + + +NEURON { + + POINT_PROCESS ProbUDFsyn2 + RANGE tau_r, tau_d + RANGE Use, u, Dep, Fac, u0 + RANGE i, g, e, gmax + NONSPECIFIC_CURRENT i + POINTER rng +} + +PARAMETER { + + tau_r = 0.2 (ms) : dual-exponential conductance profile + tau_d = 1.7 (ms) : IMPORTANT: tau_r < tau_d + Use = 1.0 (1) : Utilization of synaptic efficacy (just initial values! Use, Dep and Fac are overwritten by BlueBuilder assigned values) + Dep = 100 (ms) : relaxation time constant from depression + Fac = 10 (ms) : relaxation time constant from facilitation + e = 0 (mV) : AMPA and NMDA reversal potential + gmax = .001 (uS) : weight conversion factor (from nS to uS) + u0 = 0 :initial value of u, which is the running value of Use +} + +COMMENT +The Verbatim block is needed to generate random nos. from a uniform distribution between 0 and 1 +for comparison with Pr to decide whether to activate the synapse or not +ENDCOMMENT + +VERBATIM + +#include +#include +#include + +double nrn_random_pick(void* r); +void* nrn_random_arg(int argpos); + +ENDVERBATIM + + +ASSIGNED { + + v (mV) + i (nA) + g (uS) + factor + rng + weight_NMDA +} + +STATE { + A : state variable to construct the dual-exponential profile - decays with conductance tau_r_AMPA + B : state variable to construct the dual-exponential profile - decays with conductance tau_d_AMPA +} + +INITIAL{ + + LOCAL tp + + A = 0 + B = 0 + + + tp = (tau_r*tau_d)/(tau_d-tau_r)*log(tau_d/tau_r) :time to peak of the conductance + + factor = -exp(-tp/tau_r)+exp(-tp/tau_d) : Normalization factor - so that when t = tp, gsyn = gpeak + factor = 1/factor + +} + +BREAKPOINT { + + SOLVE state METHOD cnexp + g = gmax*(B-A) :compute time varying conductance as the difference of state variables B and A + i = g*(v-e) :compute the driving force based on the time varying conductance, membrane potential, and reversal +} + +DERIVATIVE state{ + + A' = -A/tau_r + B' = -B/tau_d +} + + +NET_RECEIVE (weight, Pv, Pr, u, tsyn (ms)){ + + INITIAL{ + Pv=1 + u=u0 + tsyn=t + } + + : calc u at event- + if (Fac > 0) { + u = u*exp(-(t - tsyn)/Fac) :update facilitation variable if Fac>0 Eq. 2 in Fuhrmann et al. + } else { + u = Use + } + if(Fac > 0){ + u = u + Use*(1-u) :update facilitation variable if Fac>0 Eq. 2 in Fuhrmann et al. + } + + + Pv = 1 - (1-Pv) * exp(-(t-tsyn)/Dep) :Probability Pv for a vesicle to be available for release, analogous to the pool of synaptic + :resources available for release in the deterministic model. Eq. 3 in Fuhrmann et al. + Pr = u * Pv :Pr is calculated as Pv * u (running value of Use) + Pv = Pv - u * Pv :update Pv as per Eq. 3 in Fuhrmann et al. + :printf("Pv = %g\n", Pv) + :printf("Pr = %g\n", Pr) + tsyn = t + + if (erand() < Pr){ + + A = A + weight*factor + B = B + weight*factor + } +} + +PROCEDURE setRNG() { +VERBATIM + { + /** + * This function takes a NEURON Random object declared in hoc and makes it usable by this mod file. + * Note that this method is taken from Brett paper as used by netstim.hoc and netstim.mod + * which points out that the Random must be in negexp(1) mode + */ + void** pv = (void**)(&_p_rng); + if( ifarg(1)) { + *pv = nrn_random_arg(1); + } else { + *pv = (void*)0; + } + } +ENDVERBATIM +} + +FUNCTION erand() { +VERBATIM + //FILE *fi; + double value; + if (_p_rng) { + /* + :Supports separate independent but reproducible streams for + : each instance. However, the corresponding hoc Random + : distribution MUST be set to Random.negexp(1) + */ + value = nrn_random_pick(_p_rng); + //fi = fopen("RandomStreamMCellRan4.txt", "w"); + //fprintf(fi,"random stream for this simulation = %lf\n",value); + //printf("random stream for this simulation = %lf\n",value); + return value; + }else{ +ENDVERBATIM + : the old standby. Cannot use if reproducible parallel sim + : independent of nhost or which host this instance is on + : is desired, since each instance on this cpu draws from + : the same stream + erand = exprand(1) +VERBATIM + } +ENDVERBATIM + erand = value +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SK.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SK.mod new file mode 100644 index 00000000..8bfa3b72 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SK.mod @@ -0,0 +1,56 @@ +: SK-type calcium-activated potassium current +: Reference : Kohler et al. 1996 + +NEURON { + SUFFIX SK + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gbar, g, ik +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (milli/liter) +} + +PARAMETER { + v (mV) + gbar = .000001 (mho/cm2) + zTau = 1 (ms) + ek (mV) + cai (mM) +} + +ASSIGNED { + zInf + ik (mA/cm2) + g (S/cm2) +} + +STATE { + z FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * z + ik = g * (v - ek) +} + +DERIVATIVE states { + rates(cai) + z' = (zInf - z) / zTau +} + +PROCEDURE rates(ca(mM)) { + if(ca < 1e-7){ + ca = ca + 1e-07 + } + zInf = 1/(1 + (0.00043 / ca)^4.8) +} + +INITIAL { + rates(cai) + z = zInf +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SK_E2.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SK_E2.mod new file mode 100755 index 00000000..6a0c48f9 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SK_E2.mod @@ -0,0 +1,56 @@ +: SK-type calcium-activated potassium current +: Reference : Kohler et al. 1996 + +NEURON { + SUFFIX SK_E2 + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gSK_E2bar, gSK_E2, ik +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (milli/liter) +} + +PARAMETER { + v (mV) + gSK_E2bar = .000001 (mho/cm2) + zTau = 1 (ms) + ek (mV) + cai (mM) +} + +ASSIGNED { + zInf + ik (mA/cm2) + gSK_E2 (mho/cm2) +} + +STATE { + z FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gSK_E2 = gSK_E2bar * z + ik = gSK_E2 * (v - ek) +} + +DERIVATIVE states { + rates(cai) + z' = (zInf - z) / zTau +} + +PROCEDURE rates(ca(mM)) { + if(ca < 1e-7 (mM)){ + ca = ca + 1e-07 (mM) + } + zInf = 1/(1 + (0.00043 (mM)/ ca)^4.8) +} + +INITIAL { + rates(cai) + z = zInf +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SKv3_1.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SKv3_1.mod new file mode 100755 index 00000000..9c4e0042 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/SKv3_1.mod @@ -0,0 +1,54 @@ +:Comment : +:Reference : : Characterization of a Shaw-related potassium channel family in rat brain, The EMBO Journal, vol.11, no.7,2473-2486 (1992) + +NEURON { + SUFFIX SKv3_1 + USEION k READ ek WRITE ik + RANGE gSKv3_1bar, gSKv3_1, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gSKv3_1bar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gSKv3_1 (S/cm2) + mInf (1) + mTau (ms) +} + +STATE { + m (1) +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gSKv3_1 = gSKv3_1bar*m + ik = gSKv3_1*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + mInf = 1/(1+exp(((v -(18.700(mV)))/(-9.700(mV))))) + mTau = 0.2*20.000(ms)/(1+exp(((v -(-46.560(mV)))/(-44.140(mV))))) + UNITSOFF + UNITSON +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/cdp5_CAM.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/cdp5_CAM.mod new file mode 100644 index 00000000..1aba233f --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/cdp5_CAM.mod @@ -0,0 +1,373 @@ +: Calcium ion accumulation with endogenous buffers, DCM and pump + +COMMENT + +The basic code of Example 9.8 and Example 9.9 from NEURON book was adapted as: + +1) Extended using parameters from Schmidt et al. 2003. +2) Pump rate was tuned according to data from Maeda et al. 1999 +3) DCM was introduced and tuned to approximate the effect of radial diffusion + +Reference: Anwar H, Hong S, De Schutter E (2010) Controlling Ca2+-activated K+ channels with models of Ca2+ buffering in Purkinje cell. Cerebellum* + +*Article available as Open Access + +PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20981513 + +Written by Haroon Anwar, Computational Neuroscience Unit, Okinawa Institute of Science and Technology, 2010. +Contact: Haroon Anwar (anwar@oist.jp) + +ENDCOMMENT + + +NEURON { + SUFFIX cdp5_CAM + USEION ca READ cao, cai, ica WRITE cai + + RANGE ica_pmp + RANGE Nannuli, Buffnull2, rf3, rf4, vrat + RANGE CAM0, CAM1C, CAM2C, CAM1N2C, CAM1N, CAM2N, CAM2N1C, CAM1C1N, CAM4 + RANGE TotalPump + +} + + +UNITS { + (mol) = (1) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + FARADAY = (faraday) (10000 coulomb) + PI = (pi) (1) +} + +PARAMETER { + Nannuli = 10.9495 (1) + celsius (degC) + + cainull = 45e-6 (mM) + mginull =.59 (mM) + +: values for a buffer compensating the diffusion + + Buffnull1 = 0 (mM) + rf1 = 0.0134329 (/ms mM) + rf2 = 0.0397469 (/ms) + + Buffnull2 = 60.9091 (mM) + rf3 = 0.1435 (/ms mM) + rf4 = 0.0014 (/ms) + +: values for benzothiazole coumarin (BTC) + BTCnull = 0 (mM) + b1 = 5.33 (/ms mM) + b2 = 0.08 (/ms) + +: values for caged compound DMNPE-4 + DMNPEnull = 0 (mM) + c1 = 5.63 (/ms mM) + c2 = 0.107e-3 (/ms) + +: values for Calbindin (2 high and 2 low affinity binding sites) + + CBnull= .16 (mM) + nf1 =43.5 (/ms mM) + nf2 =3.58e-2 (/ms) + ns1 =5.5 (/ms mM) + ns2 =0.26e-2 (/ms) + +: values for Parvalbumin + + PVnull = .08 (mM) + m1 = 1.07e2 (/ms mM) + m2 = 9.5e-4 (/ms) + p1 = 0.8 (/ms mM) + p2 = 2.5e-2 (/ms) + +: Calmodulin concentration + CAM_start = 0.03 (mM) :Pepke 2010 + +: Calmodulin Kinetic parameters. The values are the mean between max and min. + :C-lobe + Kd1C = 0.00965 (mM) : Kd - Equilibrium binding of 1st Ca2+ to CaM C-terminus + K1Coff = 0.04 (/ms) : From 0C to 1C with X ions on N-lobe + K1Con = 5.4 (/mM ms) : From 1C to 0C with X ions on N-lobe + Kd2C = 0.00105 (mM) : Kd - Equilibrium binding of 2nd Ca2+ to CaM C-terminus + K2Coff = 0.00925 (/ms) : From 1C to 2C with X ions on N-lobe + K2Con = 15 (/mM ms) : From 2C to 1C with X ions on N-lobe + + :N-lobe + Kd1N = 0.0275 (uM) : Kd - Equilibrium binding of 1st Ca2+ to CaM N-terminus + K1Noff = 2.5 (/ms) : From 0N to 1N with X ions on C-lobe + K1Non = 142.5 (/mM ms) : From 1N to 0N with X ions on C-lobe + Kd2N = 0.00615 (mM) : Kd - Equilibrium binding of 2nd Ca2+ to CaM N-terminus + K2Noff = 0.75 (/ms) : From 1N to 2N with X ions on C-lobe + K2Non = 175 (/mM ms) : From 2N to 1N with X ions on C-lobe + + + + kpmp1 = 3e-3 (/mM-ms) + kpmp2 = 1.75e-5 (/ms) + kpmp3 = 7.255e-5 (/ms) + TotalPump = 1e-9 (mol/cm2) + +} + +ASSIGNED { + diam (um) + ica (mA/cm2) + ica_pmp (mA/cm2) + parea (um) : pump area per unit length + parea2 (um) + cai (mM) + cao (mM) + mgi (mM) + vrat (1) +} + +:CONSTANT { cao = 2 (mM) } + +STATE { + : ca[0] is equivalent to cai + : ca[] are very small, so specify absolute tolerance + : let it be ~1.5 - 2 orders of magnitude smaller than baseline level + + ca (mM) <1e-3> + mg (mM) <1e-6> + + Buff1 (mM) + Buff1_ca (mM) + + Buff2 (mM) + Buff2_ca (mM) + + BTC (mM) + BTC_ca (mM) + + DMNPE (mM) + DMNPE_ca (mM) + + CB (mM) + CB_f_ca (mM) + CB_ca_s (mM) + CB_ca_ca (mM) + + PV (mM) + PV_ca (mM) + PV_mg (mM) + +:State for the Calmodulin + + CAM0 (mM) + + :C-lobe mainly + CAM1C (mM) + CAM2C (mM) + CAM1N2C (mM) + + :N-Lobe Mainly + CAM1N (mM) + CAM2N (mM) + CAM2N1C (mM) + + :One ion on C-lobe and one on N-lobe + CAM1C1N (mM) + + :CaM complete + CAM4 (mM) + + pump (mol/cm2) <1e-15> + pumpca (mol/cm2) <1e-15> + +} + +BREAKPOINT { + SOLVE state METHOD sparse +} + +LOCAL factors_done + +INITIAL { + factors() + + ca = cainull + mg = mginull + + Buff1 = ssBuff1() + Buff1_ca = ssBuff1ca() + + Buff2 = ssBuff2() + Buff2_ca = ssBuff2ca() + + BTC = ssBTC() + BTC_ca = ssBTCca() + + DMNPE = ssDMNPE() + DMNPE_ca = ssDMNPEca() + + CB = ssCB( kdf(), kds()) + CB_f_ca = ssCBfast( kdf(), kds()) + CB_ca_s = ssCBslow( kdf(), kds()) + CB_ca_ca = ssCBca( kdf(), kds()) + + PV = ssPV( kdc(), kdm()) + PV_ca = ssPVca(kdc(), kdm()) + PV_mg = ssPVmg(kdc(), kdm()) + + :Calmodulin + CAM0 = CAM_start + CAM1C = 0 + CAM2C = 0 + CAM1N2C = 0 + CAM1N = 0 + CAM2N = 0 + CAM2N1C = 0 + CAM1C1N = 0 + CAM4 = 0 + + + parea = PI*diam + parea2 = PI*(diam-0.2) + ica = 0 + ica_pmp = 0 +: ica_pmp_last = 0 + pump = TotalPump + pumpca = 0 + + cai = ca + +} + +PROCEDURE factors() { + LOCAL r, dr2 + r = 1/2 : starts at edge (half diam) + dr2 = r/(Nannuli-1)/2 : full thickness of outermost annulus, + vrat = PI*(r-dr2/2)*2*dr2 : interior half + r = r - dr2 + +} + + +LOCAL dsq, dsqvol : can't define local variable in KINETIC block + : or use in COMPARTMENT statement + +KINETIC state { + COMPARTMENT diam*diam*vrat {ca mg Buff1 Buff1_ca Buff2 Buff2_ca BTC BTC_ca DMNPE DMNPE_ca CB CB_f_ca CB_ca_s CB_ca_ca PV PV_ca PV_mg CAM0 CAM1C CAM2C CAM1N2C CAM1N CAM2N CAM2N1C CAM1C1N CAM4} + COMPARTMENT (1e10)*parea {pump pumpca} + + + :pump + ~ ca + pump <-> pumpca (kpmp1*parea*(1e10), kpmp2*parea*(1e10)) + ~ pumpca <-> pump (kpmp3*parea*(1e10), 0) + CONSERVE pump + pumpca = TotalPump * parea * (1e10) + + ica_pmp = 2*FARADAY*(f_flux - b_flux)/parea + : all currents except pump + : ica is Ca efflux + ~ ca << (-ica*PI*diam/(2*FARADAY)) + + :RADIAL DIFFUSION OF ca, mg and mobile buffers + + dsq = diam*diam + dsqvol = dsq*vrat + ~ ca + Buff1 <-> Buff1_ca (rf1*dsqvol, rf2*dsqvol) + ~ ca + Buff2 <-> Buff2_ca (rf3*dsqvol, rf4*dsqvol) + ~ ca + BTC <-> BTC_ca (b1*dsqvol, b2*dsqvol) + ~ ca + DMNPE <-> DMNPE_ca (c1*dsqvol, c2*dsqvol) + :Calbindin + ~ ca + CB <-> CB_ca_s (nf1*dsqvol, nf2*dsqvol) + ~ ca + CB <-> CB_f_ca (ns1*dsqvol, ns2*dsqvol) + ~ ca + CB_f_ca <-> CB_ca_ca (nf1*dsqvol, nf2*dsqvol) + ~ ca + CB_ca_s <-> CB_ca_ca (ns1*dsqvol, ns2*dsqvol) + + :Paravalbumin + ~ ca + PV <-> PV_ca (m1*dsqvol, m2*dsqvol) + ~ mg + PV <-> PV_mg (p1*dsqvol, p2*dsqvol) + + :Calmodulin + :C-lobe + ~ ca + CAM0 <-> CAM1C (K1Con*dsqvol, K1Coff*dsqvol) + ~ ca + CAM1C <-> CAM2C (K2Con*dsqvol, K2Coff*dsqvol) + ~ ca + CAM2C <-> CAM1N2C (K1Non*dsqvol, K1Noff*dsqvol) + ~ ca + CAM1N2C <-> CAM4 (K2Non*dsqvol, K2Noff*dsqvol) + + :N-lobe + ~ ca + CAM0 <-> CAM1N (K1Non*dsqvol, K1Noff*dsqvol) + ~ ca + CAM1N <-> CAM2N (K2Non*dsqvol, K2Noff*dsqvol) + ~ ca + CAM2N <-> CAM2N1C (K1Con*dsqvol, K1Coff*dsqvol) + ~ ca + CAM2N1C <-> CAM4 (K2Con*dsqvol, K2Coff*dsqvol) + + :Mixed C and N lobes + ~ ca + CAM1C <-> CAM1C1N (K1Non*dsqvol, K1Noff*dsqvol) + ~ ca + CAM1N <-> CAM1C1N (K1Con*dsqvol, K1Coff*dsqvol) + ~ ca + CAM1C1N <-> CAM1N2C (K2Con*dsqvol, K2Coff*dsqvol) + ~ ca + CAM1C1N <-> CAM2N1C (K2Non*dsqvol, K2Noff*dsqvol) + + cai = ca + mgi = mg + +} + +FUNCTION ssBuff1() (mM) { + ssBuff1 = Buffnull1/(1+((rf1/rf2)*cainull)) +} +FUNCTION ssBuff1ca() (mM) { + ssBuff1ca = Buffnull1/(1+(rf2/(rf1*cainull))) +} +FUNCTION ssBuff2() (mM) { + ssBuff2 = Buffnull2/(1+((rf3/rf4)*cainull)) +} +FUNCTION ssBuff2ca() (mM) { + ssBuff2ca = Buffnull2/(1+(rf4/(rf3*cainull))) +} + +FUNCTION ssBTC() (mM) { + ssBTC = BTCnull/(1+((b1/b2)*cainull)) +} + +FUNCTION ssBTCca() (mM) { + ssBTCca = BTCnull/(1+(b2/(b1*cainull))) +} + +FUNCTION ssDMNPE() (mM) { + ssDMNPE = DMNPEnull/(1+((c1/c2)*cainull)) +} + +FUNCTION ssDMNPEca() (mM) { + ssDMNPEca = DMNPEnull/(1+(c2/(c1*cainull))) +} + +FUNCTION ssCB( kdf(), kds()) (mM) { + ssCB = CBnull/(1+kdf()+kds()+(kdf()*kds())) +} +FUNCTION ssCBfast( kdf(), kds()) (mM) { + ssCBfast = (CBnull*kds())/(1+kdf()+kds()+(kdf()*kds())) +} +FUNCTION ssCBslow( kdf(), kds()) (mM) { + ssCBslow = (CBnull*kdf())/(1+kdf()+kds()+(kdf()*kds())) +} +FUNCTION ssCBca(kdf(), kds()) (mM) { + ssCBca = (CBnull*kdf()*kds())/(1+kdf()+kds()+(kdf()*kds())) +} +FUNCTION kdf() (1) { + kdf = (cainull*nf1)/nf2 +} +FUNCTION kds() (1) { + kds = (cainull*ns1)/ns2 +} +FUNCTION kdc() (1) { + kdc = (cainull*m1)/m2 +} +FUNCTION kdm() (1) { + kdm = (mginull*p1)/p2 +} +FUNCTION ssPV( kdc(), kdm()) (mM) { + ssPV = PVnull/(1+kdc()+kdm()) +} +FUNCTION ssPVca( kdc(), kdm()) (mM) { + ssPVca = (PVnull*kdc())/(1+kdc()+kdm()) +} +FUNCTION ssPVmg( kdc(), kdm()) (mM) { + ssPVmg = (PVnull*kdm())/(1+kdc()+kdm()) +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/epsp.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/epsp.mod new file mode 100644 index 00000000..d9473547 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/epsp.mod @@ -0,0 +1,69 @@ +: this model is built-in to neuron with suffix epsp +: Schaefer et al. 2003 + +COMMENT +modified from syn2.mod +injected current with exponential rise and decay current defined by + i = 0 for t < onset and + i=amp*((1-exp(-(t-onset)/tau0))-(1-exp(-(t-onset)/tau1))) + for t > onset + + compare to experimental current injection: + i = - amp*(1-exp(-t/t1))*(exp(-t/t2)) + + -> tau1==t2 tau0 ^-1 = t1^-1 + t2^-1 +ENDCOMMENT + +INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)} + +NEURON { + POINT_PROCESS epsp + RANGE onset, tau0, tau1, imax, i, myv + NONSPECIFIC_CURRENT i +} +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + onset=0 (ms) + tau0=0.2 (ms) + tau1=3.0 (ms) + imax=0 (nA) + v (mV) +} + +ASSIGNED { i (nA) myv (mV)} + +LOCAL a[2] +LOCAL tpeak +LOCAL adjust +LOCAL amp + +BREAKPOINT { + myv = v + i = curr(t) +} + +FUNCTION myexp(x) { + if (x < -100) { + myexp = 0 + }else{ + myexp = exp(x) + } +} + +FUNCTION curr(x) { + tpeak=tau0*tau1*log(tau0/tau1)/(tau0-tau1) + adjust=1/((1-myexp(-tpeak/tau0))-(1-myexp(-tpeak/tau1))) + amp=adjust*imax + if (x < onset) { + curr = 0 + }else{ + a[0]=1-myexp(-(x-onset)/tau0) + a[1]=1-myexp(-(x-onset)/tau1) + curr = -amp*(a[0]-a[1]) + } +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/exp1isyn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/exp1isyn.mod new file mode 100755 index 00000000..e8700401 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/exp1isyn.mod @@ -0,0 +1,52 @@ +COMMENT +Current-based version of the conductance-based model exp1syn (refer to the exp1syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + +NEURON { + POINT_PROCESS exp1isyn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*e +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/exp1syn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/exp1syn.mod new file mode 100755 index 00000000..6763e687 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/exp1syn.mod @@ -0,0 +1,42 @@ +NEURON { + POINT_PROCESS exp1syn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v-e) +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp1syn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp1syn.mod new file mode 100755 index 00000000..3490c587 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp1syn.mod @@ -0,0 +1,75 @@ +COMMENT +A model (# 1 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp1syn + RANGE e, i, tau_r, p0, tau_1 + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + g +} + +INITIAL { + n=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp2syn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp2syn.mod new file mode 100755 index 00000000..ce1d1625 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp2syn.mod @@ -0,0 +1,95 @@ +COMMENT +A model (# 2 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp2syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) + (uS) = (microsiemens) + +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_1 = baseline level of release probability + tau_1 = 5 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + tau_r + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p0 +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + tau_r' = (tau_r0-tau_r)/tau_FDR +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp3syn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp3syn.mod new file mode 100755 index 00000000..31869840 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp3syn.mod @@ -0,0 +1,102 @@ +COMMENT +A model (# 3 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp3syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D + a_D = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + tau_r + D + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 + D=1 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0*D + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p0*n*D + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp4syn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp4syn.mod new file mode 100755 index 00000000..1ba964be --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp4syn.mod @@ -0,0 +1,83 @@ +COMMENT +A model (# 4 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by +solving Eqs (3,4) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + p = release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp4syn + RANGE e, i, tau_r, p0, tau_1,tau_f + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + p + g +} + +INITIAL { + n=1 + p=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + p' = 0 +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + p = p + p0*(1-p) + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp5isyn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp5isyn.mod new file mode 100755 index 00000000..d6b6c105 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp5isyn.mod @@ -0,0 +1,113 @@ +COMMENT +Current-based version of the conductance-based model stp5syn (refer to the stp5syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp5isyn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of pb + pbtilde = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + p + tau_r + D + pb + g +} + +INITIAL { + n=1 + pb=pbtilde + p=pb + tau_r=tau_r0 + D=1 + g=0 + +} + +BREAKPOINT { + SOLVE state METHOD cnexp +: i = g*(v - e) + i = g*e + Pmax = n*p*D + +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp5syn.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp5syn.mod new file mode 100755 index 00000000..621fe9e1 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/stp5syn.mod @@ -0,0 +1,131 @@ +COMMENT +A model (# 5 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,4,5,8,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." +This file could be used to simulate simpler models by setting to zero the parameters in the unused equations(e.g, a_D,a_i,a_f). + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + pb = baseline vesicle release probability + p = vesicle release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where w is the static synaptic weight and Pmax is the activity-dependent factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1. + +Implemented by Sergey L. Gratiy, + +ENDCOMMENT + +: Declaring parameters as RANGE allows them to change as a function of a position alogn the cell +NEURON { + POINT_PROCESS stp5syn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +: Declaration of the default values of parameters +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of p0 + pbtilde = 0.5 + +} +: Declaration of dependent and external variables that collectively are called ASSIGNED +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +: Declaration of the state variables +STATE { + n + p + tau_r + D + pb + g +} + +: Initial conditions for the state variables +INITIAL { + n=1 + p=1 + tau_r=tau_r0 + D=1 + pb=pbtilde + g=0 + +} + +: Integration method + assignment statements +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p*D +} + +: Definition of teh dynamics between the presynaptic activations +DERIVATIVE state { + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + g' = -g/tau_1 + +} + +: This block defines what happens to the state variables at the moment presynaptic activation +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/vecevent.mod b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/vecevent.mod new file mode 100755 index 00000000..503dfd2e --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/mechanisms/modfiles/vecevent.mod @@ -0,0 +1,71 @@ +: Vector stream of events + +NEURON { + ARTIFICIAL_CELL VecStim +} + +ASSIGNED { + index + etime (ms) + space +} + +INITIAL { + index = 0 + element() + if (index > 0) { + net_send(etime - t, 1) + } +} + +NET_RECEIVE (w) { + if (flag == 1) { + net_event(t) + element() + if (index > 0) { + net_send(etime - t, 1) + } + } +} + +VERBATIM +extern double* vector_vec(); +extern int vector_capacity(); +extern void* vector_arg(); +ENDVERBATIM + +PROCEDURE element() { +VERBATIM + { void* vv; int i, size; double* px; + i = (int)index; + if (i >= 0) { + vv = *((void**)(&space)); + if (vv) { + size = vector_capacity(vv); + px = vector_vec(vv); + if (i < size) { + etime = px[i]; + index += 1.; + }else{ + index = -1.; + } + }else{ + index = -1.; + } + } + } +ENDVERBATIM +} + +PROCEDURE play() { +VERBATIM + void** vv; + vv = (void**)(&space); + *vv = (void*)0; + if (ifarg(1)) { + *vv = vector_arg(1); + } +ENDVERBATIM +} + + diff --git a/docs/tutorial/01_opt_external_models/components/morphologies/HL5PN1.swc b/docs/tutorial/01_opt_external_models/components/morphologies/HL5PN1.swc new file mode 100644 index 00000000..11523c29 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/morphologies/HL5PN1.swc @@ -0,0 +1,12283 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): D:/BatchProcessing/681753346/reconstructions-681753346/mylinker.ano.swc_sorted.swc_part1_nosmooth.swc_assembled.swc_interpolated.swc +# id,type,x,y,z,r,pid +1 1 656.0142 1506.5908 37.0894 6.5548 -1 +2 2 650.3423 1507.895 36.4302 0.1144 1 +3 2 649.2086 1507.9407 36.7791 0.1144 2 +4 2 648.0749 1507.9865 37.128 0.1144 3 +5 2 647.0121 1508.2496 37.1795 0.1144 4 +6 2 646.0751 1508.8788 36.7091 0.1144 5 +7 2 645.1382 1509.508 36.2387 0.1144 6 +8 2 644.2013 1510.1372 35.7683 0.1144 7 +9 2 643.2643 1510.755 35.2979 0.1144 8 +10 2 642.2439 1511.224 34.9362 0.1144 9 +11 2 641.1571 1511.5672 34.6632 0.1144 10 +12 2 640.0691 1511.899 34.3899 0.1144 11 +13 2 638.9812 1512.2422 34.1166 0.1144 12 +14 2 637.9161 1512.6197 33.7394 0.1144 13 +15 2 636.8831 1513.0544 33.2122 0.1144 14 +16 2 635.8512 1513.5006 32.6847 0.1144 15 +17 2 634.8182 1513.9467 32.1574 0.1144 16 +18 2 633.7771 1514.37 31.6912 0.1144 17 +19 2 632.6709 1514.656 31.6912 0.1144 18 +20 2 631.5635 1514.9534 31.6912 0.1144 19 +21 2 630.4664 1515.2394 31.4994 0.1144 20 +22 2 629.3842 1515.5483 30.9694 0.1144 21 +23 2 628.3706 1515.9602 30.4343 0.1144 22 +24 2 627.5915 1516.7724 29.8833 0.1144 23 +25 2 626.8079 1517.5732 29.4277 0.1144 24 +26 2 625.9968 1518.3854 29.4277 0.1144 25 +27 2 625.1857 1519.1862 29.4277 0.1144 26 +28 2 624.3643 1519.8612 28.6734 0.1144 27 +29 2 623.5349 1520.4446 27.3689 0.1144 28 +30 2 622.7055 1521.0281 26.0641 0.1144 29 +31 2 621.8155 1521.7259 26.3729 0.1144 30 +32 2 620.9243 1522.4238 26.7221 0.1144 31 +33 2 620.032 1523.133 27.0715 0.1144 32 +34 2 620.04 1524.0597 27.9087 0.1144 33 +35 2 619.8684 1525.0893 28.2957 0.1144 34 +36 2 619.3055 1526.0274 28.2957 0.1144 35 +37 2 618.1982 1526.3134 28.2957 0.1144 36 +38 2 617.0908 1526.6108 28.2957 0.1144 37 +39 2 616.2591 1527.3086 28.2957 0.1144 38 +40 2 615.5681 1528.2238 28.2957 0.1144 39 +41 2 614.8771 1529.1276 28.2957 0.1144 40 +42 2 614.1964 1530.0542 28.2957 0.1144 41 +43 2 613.5261 1530.9809 28.2957 0.1144 42 +44 2 612.8545 1531.9075 28.2957 0.1144 43 +45 2 612.1189 1532.777 28.2957 0.1144 44 +46 2 611.3616 1533.635 28.2957 0.1144 45 +47 2 610.6969 1534.5502 28.1039 0.1144 46 +48 2 610.2165 1535.5569 27.5299 0.1144 47 +49 2 609.7348 1536.5636 26.9556 0.1144 48 +50 2 609.2143 1537.5703 26.5997 0.1584 49 +51 2 608.4707 1538.3826 26.4863 0.2288 50 +52 2 607.6722 1539.1262 26.2021 0.2288 51 +53 2 607.3839 1540.2015 26.0436 0.2288 52 +54 2 606.9721 1541.1396 26.04 0.2288 53 +55 2 606.2777 1541.9976 26.0397 0.2288 54 +56 2 605.8441 1543.0501 25.7673 0.2288 55 +57 2 605.5009 1544.114 25.1717 0.2288 56 +58 2 605.0033 1545.1093 24.92 0.2288 57 +59 2 604.3306 1546.0359 24.92 0.2288 58 +60 2 603.8158 1547.0426 24.92 0.2288 59 +61 2 603.4463 1548.1294 24.92 0.2288 60 +62 2 602.8628 1549.0904 24.92 0.2288 61 +63 2 602.3137 1550.0857 24.92 0.2288 62 +64 2 601.9236 1551.161 24.92 0.2288 63 +65 2 601.4191 1552.1792 24.9217 0.2288 64 +66 2 600.918 1553.163 25.5626 0.2288 65 +67 2 600.3266 1554.124 25.7499 0.2288 66 +68 2 599.3462 1554.6502 25.4979 0.2288 67 +69 2 598.4195 1555.3138 25.48 0.2288 68 +70 2 597.7835 1556.2633 25.48 0.2288 69 +71 2 597.5123 1557.1556 25.4769 0.2288 70 +72 2 597.1188 1558.2081 25.0018 0.2288 71 +73 2 596.7276 1559.2834 24.92 0.2288 72 +74 2 596.5754 1560.4046 24.92 0.2288 73 +75 2 596.3866 1561.5371 24.9189 0.2288 74 +76 2 595.6385 1562.3494 24.4479 0.2288 75 +77 2 594.8011 1563.1273 24.3617 0.2288 76 +78 2 594.3366 1564.1683 24.1391 0.2288 77 +79 2 593.7177 1564.8204 23.7938 0.2288 78 +80 2 592.8837 1565.5754 23.3022 0.2288 79 +81 2 592.2522 1566.525 23.2655 0.2288 80 +82 2 592.0806 1567.6346 23.4962 0.2288 81 +83 2 592.0246 1568.7786 23.4189 0.2288 82 +84 2 591.909 1569.4536 21.28 0.2288 83 +85 2 590.8954 1570.529 21.28 0.2288 84 +86 2 590.0203 1571.2382 21.278 0.2288 85 +87 2 588.9758 1571.6958 21.1898 0.2288 86 +88 2 588.2139 1572.3937 21.0 0.2288 87 +89 2 588.0137 1573.5148 21.0 0.2288 88 +90 2 587.5081 1574.3957 21.0 0.2288 89 +91 2 586.4293 1574.7274 21.0 0.2288 90 +92 2 585.4592 1575.1736 21.0 0.2288 91 +93 2 584.6584 1575.9858 21.0 0.2288 92 +94 2 584.1447 1576.9697 21.0 0.2288 93 +95 2 583.8049 1578.0565 21.0 0.2288 94 +96 2 583.1677 1578.9831 21.0 0.2288 95 +97 2 582.4768 1579.8869 21.0 0.2288 96 +98 2 581.5524 1580.5046 20.8496 0.2288 97 +99 2 580.5537 1581.0652 20.7376 0.2288 98 +100 2 579.5001 1581.477 20.419 0.2288 99 +101 2 578.4991 1582.0147 20.1956 0.2288 100 +102 2 577.6353 1582.7469 20.3949 0.2288 101 +103 2 577.1514 1583.765 20.44 0.2288 102 +104 2 577.1583 1584.8976 20.44 0.2288 103 +105 2 577.2853 1586.0416 20.44 0.2288 104 +106 2 576.9078 1587.1055 20.4445 0.2288 105 +107 2 576.1584 1587.8834 21.2472 0.2288 106 +108 2 575.2821 1588.6156 21.5578 0.2288 107 +109 2 574.3429 1589.2562 21.7479 0.2288 108 +110 2 573.3968 1589.8969 21.84 0.2288 109 +111 2 572.5857 1590.7091 21.84 0.2288 110 +112 2 571.8879 1591.6129 21.8406 0.2288 111 +113 2 571.4829 1592.6425 21.8814 0.2288 112 +114 2 570.4407 1593.0772 22.12 0.2288 113 +115 2 569.3127 1593.2602 22.12 0.2288 114 +116 2 568.2351 1593.6149 22.12 0.2288 115 +117 2 567.265 1594.2212 22.1208 0.2288 116 +118 2 566.2525 1594.2555 21.565 0.2288 117 +119 2 565.1349 1594.0153 21.562 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1676.6578 30.3131 0.1144 789 +791 2 518.9024 1677.3786 29.8746 0.1144 790 +792 2 518.0375 1678.1107 29.4364 0.1144 791 +793 2 517.1715 1678.8314 28.9979 0.1144 792 +794 2 516.3066 1679.5636 28.5594 0.1144 793 +795 2 515.555 1680.3873 28.4276 0.1144 794 +796 2 514.975 1681.3597 28.7585 0.1144 795 +797 2 514.395 1682.3435 29.0898 0.1144 796 +798 2 513.8162 1683.3159 29.4207 0.1144 797 +799 2 512.9067 1683.9909 29.0612 0.1144 798 +800 2 511.9903 1684.6544 28.6868 0.1144 799 +801 2 511.0728 1685.3294 28.3125 0.1144 800 +802 2 510.1565 1685.9929 27.9381 0.1144 801 +803 2 509.2402 1686.6564 27.5638 0.1144 802 +804 2 508.3238 1687.3314 27.1894 0.1144 803 +805 2 507.4063 1687.9949 26.815 0.1144 804 +806 2 506.49 1688.6584 26.4407 0.1144 805 +807 2 505.5736 1689.3334 26.0663 0.1144 806 +808 2 504.7088 1690.077 26.0322 0.1144 807 +809 2 503.8485 1690.832 26.0322 0.1144 808 +810 2 502.9893 1691.587 26.0322 0.1144 809 +811 2 502.1291 1692.3421 26.0322 0.1144 810 +812 2 501.088 1692.7882 25.8532 0.1144 811 +813 2 500.0207 1693.1886 25.6488 0.1144 812 +814 2 498.9545 1693.6005 25.4444 0.1144 813 +815 2 497.8871 1694.0009 25.2403 0.1144 814 +816 2 496.8209 1694.4127 25.0359 0.1144 815 +817 2 495.7913 1694.8932 24.9004 0.1144 816 +818 2 494.8372 1695.5224 24.9004 0.1144 817 +819 2 493.8831 1696.1516 24.9004 0.1144 818 +820 2 492.9279 1696.7808 24.9004 0.1144 819 +821 2 492.198 1697.5816 24.9715 0.1144 820 +822 2 491.8033 1698.657 25.1493 0.1144 821 +823 2 491.4098 1699.7323 25.3271 0.1144 822 +824 2 491.0162 1700.7962 25.5052 0.1144 823 +825 2 490.6227 1701.8716 25.683 0.1144 824 +826 2 490.2292 1702.947 25.8608 0.1144 825 +827 2 489.8356 1704.0109 26.0386 0.1144 826 +828 2 489.4421 1705.0862 26.2167 0.1144 827 +829 2 489.0486 1706.1616 26.3945 0.1144 828 +830 2 488.6539 1707.2255 26.5723 0.1144 829 +831 2 488.2603 1708.3009 26.7504 0.1144 830 +832 2 487.8668 1709.3762 26.9282 0.1144 831 +833 2 487.4733 1710.4516 27.106 0.1144 832 +834 2 487.2204 1711.5613 27.0864 0.1144 833 +835 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24.9004 0.1144 856 +858 2 481.5679 1738.2394 24.9004 0.1144 857 +859 2 481.1286 1739.2918 24.9004 0.1144 858 +860 2 480.6894 1740.3443 24.9004 0.1144 859 +861 2 480.2489 1741.3968 24.9004 0.1144 860 +862 2 479.8176 1742.4607 24.796 0.1144 861 +863 2 479.3966 1743.5246 24.5655 0.1144 862 +864 2 478.9756 1744.5771 24.3351 0.1144 863 +865 2 478.5546 1745.641 24.1046 0.1144 864 +866 2 478.1337 1746.6935 23.8742 0.1144 865 +867 2 477.7824 1747.7803 23.7684 0.1144 866 +868 2 477.4919 1748.89 23.7684 0.1144 867 +869 2 477.2002 1749.9997 23.7684 0.1144 868 +870 2 476.341 1750.6518 23.7684 0.1144 869 +871 2 475.3309 1751.1894 23.7684 0.1144 870 +872 2 474.2921 1751.6585 23.7684 0.1144 871 +873 2 473.219 1752.0589 23.7684 0.1144 872 +874 2 472.146 1752.4593 23.7684 0.1144 873 +875 2 471.1347 1752.9741 23.7684 0.1144 874 +876 2 470.1909 1753.6147 23.7684 0.1144 875 +877 2 469.2471 1754.2668 23.7684 0.1144 876 +878 2 468.3033 1754.9074 23.7684 0.1144 877 +879 2 467.3595 1755.5595 23.7684 0.1144 878 +880 2 466.4145 1756.2002 23.7684 0.1144 879 +881 2 465.4707 1756.8522 23.7684 0.1144 880 +882 2 464.4205 1757.2069 23.4301 0.1144 881 +883 2 463.3154 1757.4128 22.9183 0.1144 882 +884 2 462.2012 1757.5043 22.8586 0.1144 883 +885 2 461.0766 1757.4357 23.3526 0.1144 884 +886 2 460.0116 1757.5272 23.7376 0.1144 885 +887 2 459.2645 1758.3852 23.5421 0.1144 886 +888 2 458.5175 1759.2546 23.347 0.1144 887 +889 2 457.7705 1760.1126 23.1518 0.1144 888 +890 2 457.0234 1760.9821 22.9564 0.1144 889 +891 2 456.2752 1761.8401 22.7612 0.1144 890 +892 2 455.9103 1762.7782 22.8085 0.1144 891 +893 2 456.2169 1763.865 23.2823 0.1144 892 +894 2 456.5235 1764.9518 23.7563 0.1144 893 +895 2 456.8301 1766.0386 24.2304 0.1144 894 +896 2 457.1367 1767.1254 24.7041 0.1144 895 +897 2 457.0669 1768.2122 24.7705 0.1144 896 +898 2 456.7294 1769.299 24.5484 0.1144 897 +899 2 456.3931 1770.3858 24.3267 0.1144 898 +900 2 456.0567 1771.484 24.1049 0.1144 899 +901 2 455.7204 1772.5708 23.8829 0.1144 900 +902 2 455.0294 1773.3258 23.7684 0.1144 901 +903 2 453.9621 1773.7377 23.7684 0.1144 902 +904 2 452.8936 1774.1495 23.7684 0.1144 903 +905 2 451.8251 1774.5614 23.7684 0.1144 904 +906 2 450.7566 1774.9618 23.7684 0.1144 905 +907 2 449.687 1775.3736 23.7684 0.1144 906 +908 2 448.6162 1775.774 23.7684 0.1144 907 +909 2 447.5442 1776.1744 23.7684 0.1144 908 +910 2 446.5696 1776.7235 23.7684 0.1144 909 +911 2 445.7573 1777.5358 23.7684 0.1144 910 +912 2 444.9462 1778.3366 23.7684 0.1144 911 +913 2 444.1351 1779.1488 23.756 0.1144 912 +914 2 443.3286 1779.961 23.6748 0.1144 913 +915 2 442.5221 1780.7618 23.5934 0.1144 914 +916 2 441.7156 1781.5741 23.5122 0.1144 915 +917 2 440.9102 1782.3863 23.4307 0.1144 916 +918 2 440.1037 1783.1986 23.3495 0.1144 917 +919 2 439.2971 1784.0108 23.268 0.1144 918 +920 2 438.4906 1784.823 23.1868 0.1144 919 +921 2 437.6841 1785.6353 23.1053 0.1144 920 +922 2 436.8776 1786.4361 23.0241 0.1144 921 +923 2 436.0711 1787.2483 22.9426 0.1144 922 +924 2 435.2645 1788.0606 22.8614 0.1144 923 +925 2 434.458 1788.8728 22.78 0.1144 924 +926 2 433.6515 1789.685 22.6985 0.1144 925 +927 2 432.829 1790.4744 22.6366 0.1144 926 +928 2 431.9538 1791.218 22.6366 0.1144 927 +929 2 431.0786 1791.9502 22.6366 0.1144 928 +930 2 430.2023 1792.6823 22.6366 0.1144 929 +931 2 429.3272 1793.4259 22.6366 0.1144 930 +932 2 428.452 1794.1581 22.6366 0.1144 931 +933 2 427.5757 1794.9017 22.6366 0.1144 932 +934 2 426.7314 1795.6567 22.5856 0.1144 933 +935 2 425.9993 1796.5376 22.3496 0.1144 934 +936 2 425.2671 1797.407 22.1133 0.1144 935 +937 2 424.5361 1798.2879 21.8772 0.1144 936 +938 2 423.804 1799.1574 21.6409 0.1144 937 +939 2 423.264 1800.1298 21.5048 0.1144 938 +940 2 422.9848 1801.2394 21.5048 0.1144 939 +941 2 422.7057 1802.3491 21.5048 0.1144 940 +942 2 422.4277 1803.4588 21.5048 0.1144 941 +943 2 422.0674 1804.5342 21.5048 0.1144 942 +944 2 421.373 1805.4379 21.5048 0.1144 943 +945 2 420.6785 1806.3531 21.5048 0.1144 944 +946 2 419.9841 1807.2569 21.5048 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410.0302 1830.5373 22.96 0.1144 968 +970 2 409.6595 1831.6126 22.96 0.1144 969 +971 2 409.2877 1832.6994 22.96 0.1144 970 +972 2 408.9159 1833.7862 22.96 0.1144 971 +973 2 408.5441 1834.8616 22.96 0.1144 972 +974 2 408.0865 1835.9026 22.96 0.1144 973 +975 2 407.5386 1836.9094 22.96 0.1144 974 +976 2 406.9917 1837.9161 22.96 0.1144 975 +977 2 406.3671 1838.8656 23.1997 0.1144 976 +978 2 405.7288 1839.8151 23.485 0.1144 977 +979 2 405.0893 1840.7532 23.77 0.1144 978 +980 2 404.4509 1841.6913 24.0551 0.1144 979 +981 2 403.8114 1842.6408 24.3401 0.1144 980 +982 2 403.1731 1843.5789 24.6252 0.1144 981 +983 2 402.5336 1844.517 24.9102 0.1144 982 +984 2 401.8941 1845.455 25.1952 0.1144 983 +985 2 401.2557 1846.4046 25.4806 0.1144 984 +986 2 400.6162 1847.3426 25.7656 0.1144 985 +987 2 399.9779 1848.2807 26.0506 0.1144 986 +988 2 399.3384 1849.2302 26.3357 0.1144 987 +989 2 398.7 1850.1683 26.6207 0.1144 988 +990 2 398.0605 1851.1064 26.9058 0.1144 989 +991 2 397.4222 1852.0559 27.1908 0.1144 990 +992 2 396.7827 1852.994 27.4761 0.1144 991 +993 2 396.1443 1853.9321 27.7612 0.1144 992 +994 2 395.5048 1854.8816 28.0462 0.1144 993 +995 2 394.8665 1855.8197 28.3312 0.1144 994 +996 2 394.227 1856.7578 28.6163 0.1144 995 +997 2 393.5886 1857.7073 28.9013 0.1144 996 +998 2 392.9491 1858.6454 29.1866 0.1144 997 +999 2 392.3096 1859.5834 29.4717 0.1144 998 +1000 2 391.6713 1860.533 29.7567 0.1144 999 +1001 2 390.6954 1860.9334 29.848 0.1144 1000 +1002 2 389.5617 1861.0821 29.848 0.1144 1001 +1003 2 388.428 1861.2422 29.848 0.1144 1002 +1004 2 387.2943 1861.391 29.848 0.1144 1003 +1005 2 386.1606 1861.5397 29.848 0.1144 1004 +1006 2 385.0269 1861.6998 29.848 0.1144 1005 +1007 2 383.8932 1861.8486 29.848 0.1144 1006 +1008 2 382.7595 1862.0087 29.848 0.1144 1007 +1009 2 381.6258 1862.1574 29.848 0.1144 1008 +1010 2 380.4921 1862.3062 29.848 0.1144 1009 +1011 2 379.3584 1862.4663 29.848 0.1144 1010 +1012 2 378.2247 1862.615 29.848 0.1144 1011 +1013 2 377.091 1862.7638 29.848 0.1144 1012 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1034 +1036 2 351.0673 1866.619 29.848 0.1144 1035 +1037 2 349.9404 1866.8135 29.848 0.1144 1036 +1038 2 348.8136 1867.0194 29.848 0.1144 1037 +1039 2 347.6868 1867.2139 29.848 0.1144 1038 +1040 2 346.5599 1867.4084 29.848 0.1144 1039 +1041 2 345.4331 1867.6029 29.848 0.1144 1040 +1042 2 344.3062 1867.8088 29.848 0.1144 1041 +1043 2 343.1794 1868.0033 29.848 0.1144 1042 +1044 2 342.0514 1868.1978 29.848 0.1144 1043 +1045 2 340.9246 1868.3922 29.848 0.1144 1044 +1046 2 339.7977 1868.5867 29.848 0.1144 1045 +1047 2 338.6709 1868.7926 29.848 0.1144 1046 +1048 2 337.5441 1868.9871 29.848 0.1144 1047 +1049 2 336.4172 1869.1816 29.848 0.1144 1048 +1050 2 335.2904 1869.3761 29.848 0.1144 1049 +1051 2 334.1635 1869.582 29.848 0.1144 1050 +1052 2 333.0367 1869.7765 29.848 0.1144 1051 +1053 2 331.9099 1869.971 29.848 0.1144 1052 +1054 2 559.9994 1615.5797 27.4386 0.1144 713 +1055 2 560.7705 1616.4034 27.552 0.1144 1054 +1056 2 561.8504 1616.7809 27.552 0.1144 1055 +1057 2 562.7736 1617.3071 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0.1144 1955 +1957 2 625.1983 1607.9034 26.4678 0.1144 1956 +1958 2 626.1341 1607.2742 26.9391 0.1144 1957 +1959 2 627.1625 1606.8738 26.934 0.1144 1958 +1960 2 628.2745 1606.6794 26.4944 0.1144 1959 +1961 2 629.3865 1606.4734 26.0548 0.1144 1960 +1962 2 630.4996 1606.279 25.6152 0.1144 1961 +1963 2 631.6116 1606.0845 25.1756 0.1144 1962 +1964 2 632.7361 1605.9586 24.9368 0.1144 1963 +1965 2 633.879 1605.9472 25.0337 0.1144 1964 +1966 2 635.0218 1605.9472 25.1308 0.1144 1965 +1967 2 636.1658 1605.9358 25.228 0.1144 1966 +1968 2 637.3087 1605.9358 25.3252 0.1144 1967 +1969 2 638.4527 1605.9243 25.4223 0.1144 1968 +1970 2 639.5955 1605.9243 25.5192 0.1144 1969 +1971 2 640.7384 1605.9129 25.6164 0.1144 1970 +1972 2 641.8824 1605.9129 25.7135 0.1144 1971 +1973 2 643.0252 1605.9014 25.8104 0.1144 1972 +1974 2 644.1681 1605.9014 25.9076 0.1144 1973 +1975 2 645.3121 1605.89 26.0047 0.1144 1974 +1976 2 646.4275 1605.6841 26.0322 0.1144 1975 +1977 2 647.5337 1605.3866 26.0322 0.1144 1976 +1978 2 648.6388 1605.0892 26.0322 0.1144 1977 +1979 2 649.744 1604.7918 26.0322 0.1144 1978 +1980 2 650.8491 1604.4943 26.0322 0.1144 1979 +1981 2 651.9805 1604.4714 26.0322 0.1144 1980 +1982 2 653.121 1604.563 26.0322 0.1144 1981 +1983 2 654.2616 1604.643 26.0322 0.1144 1982 +1984 2 655.4022 1604.7346 26.0322 0.1144 1983 +1985 2 656.5439 1604.8146 26.0322 0.1144 1984 +1986 2 657.6845 1604.8947 26.0322 0.1144 1985 +1987 2 658.825 1604.9862 26.0322 0.1144 1986 +1988 2 659.937 1605.2379 26.0322 0.1144 1987 +1989 2 661.0444 1605.5239 26.0322 0.1144 1988 +1990 2 662.1518 1605.8214 26.0322 0.1144 1989 +1991 2 663.2592 1606.1074 26.0322 0.1144 1990 +1992 2 664.3654 1606.3018 26.0322 0.1144 1991 +1993 2 665.4717 1606.0044 26.0322 0.1144 1992 +1994 2 666.5768 1605.707 26.0322 0.1144 1993 +1995 2 667.6922 1605.6383 26.1702 0.1144 1994 +1996 2 668.8179 1605.8099 26.4594 0.1144 1995 +1997 2 669.9424 1605.9701 26.7492 0.1144 1996 +1998 2 671.0681 1606.1417 27.0388 0.1144 1997 +1999 2 672.1972 1606.3133 27.1639 0.1144 1998 +2000 2 673.3275 1606.4849 27.1639 0.1144 1999 +2001 2 674.4578 1606.6565 27.1639 0.1144 2000 +2002 2 675.5892 1606.8281 27.1639 0.1144 2001 +2003 2 676.7195 1607.0111 27.1639 0.1144 2002 +2004 2 677.8497 1607.1827 27.1639 0.1144 2003 +2005 2 678.98 1607.3543 27.1639 0.1144 2004 +2006 2 680.116 1607.4458 27.3143 0.1144 2005 +2007 2 681.2554 1607.4802 27.5551 0.1144 2006 +2008 2 682.3949 1607.5145 27.7959 0.1144 2007 +2009 2 683.5343 1607.5488 28.0367 0.1144 2008 +2010 2 684.6737 1607.5831 28.2775 0.1144 2009 +2011 2 685.8166 1607.5602 28.2957 0.1144 2010 +2012 2 686.9606 1607.5374 28.2957 0.1144 2011 +2013 2 688.1046 1607.5145 28.2957 0.1144 2012 +2014 2 689.2486 1607.4916 28.2957 0.1144 2013 +2015 2 690.3914 1607.4687 28.2957 0.1144 2014 +2016 2 691.5354 1607.4458 28.2957 0.1144 2015 +2017 2 692.6565 1607.2514 28.1697 0.1144 2016 +2018 2 693.7731 1606.9997 28.016 0.1144 2017 +2019 2 694.8896 1606.7594 27.862 0.1144 2018 +2020 2 696.005 1606.5192 27.7082 0.1144 2019 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767.0875 1600.353 33.4972 0.1144 2084 +2086 2 768.2177 1600.2844 33.8993 0.1144 2085 +2087 2 769.348 1600.2158 34.3014 0.1144 2086 +2088 2 770.4783 1600.1471 34.7038 0.1144 2087 +2089 2 771.6086 1600.0785 35.0868 0.1144 2088 +2090 2 772.7526 1600.0899 35.0868 0.1144 2089 +2091 2 773.8966 1600.1128 35.0868 0.1144 2090 +2092 2 775.0406 1600.1242 35.0868 0.1144 2091 +2093 2 776.1846 1600.1471 35.0868 0.1144 2092 +2094 2 777.3286 1600.1586 35.0868 0.1144 2093 +2095 2 778.4726 1600.1814 35.0868 0.1144 2094 +2096 2 779.6154 1600.1929 35.0868 0.1144 2095 +2097 2 780.7571 1600.2272 35.1652 0.1144 2096 +2098 2 781.8897 1600.3073 35.5043 0.1144 2097 +2099 2 783.0222 1600.3874 35.8431 0.1144 2098 +2100 2 784.1548 1600.4789 36.1822 0.1144 2099 +2101 2 785.2874 1600.559 36.521 0.1144 2100 +2102 2 786.4199 1600.639 36.86 0.1144 2101 +2103 2 787.5525 1600.7306 37.1988 0.1144 2102 +2104 2 788.4986 1600.3073 37.3503 0.1144 2103 +2105 2 789.2948 1599.495 37.3503 0.1144 2104 +2106 2 790.091 1598.6714 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938.2733 1448.7387 95.389 0.1144 3484 +3486 2 937.532 1447.9265 94.6487 0.1144 3485 +3487 2 936.7919 1447.1028 93.9086 0.1144 3486 +3488 2 936.0505 1446.2906 93.1683 0.1144 3487 +3489 2 935.3092 1445.4783 92.428 0.1144 3488 +3490 2 934.5679 1444.6546 91.6877 0.1144 3489 +3491 2 933.8266 1443.8424 90.9476 0.1144 3490 +3492 2 933.0853 1443.0187 90.2073 0.1144 3491 +3493 2 932.3451 1442.2065 89.467 0.1144 3492 +3494 2 931.6038 1441.3828 88.727 0.1144 3493 +3495 2 930.8625 1440.5706 87.9866 0.1144 3494 +3496 2 930.1212 1439.7469 87.2463 0.1144 3495 +3497 2 929.3799 1438.9346 86.5063 0.1144 3496 +3498 2 928.6386 1438.1224 85.766 0.1144 3497 +3499 2 927.8984 1437.2987 85.0256 0.1144 3498 +3500 2 927.4271 1436.4178 83.704 0.1144 3499 +3501 2 926.9866 1435.5255 82.318 0.1144 3500 +3502 2 926.545 1434.6332 80.932 0.1144 3501 +3503 2 926.1046 1433.7409 79.546 0.1144 3502 +3504 2 925.663 1432.8486 78.16 0.1144 3503 +3505 2 925.2226 1431.9677 76.774 0.1144 3504 +3506 2 924.741 1431.0525 75.6143 0.1144 3505 +3507 2 924.1529 1430.103 75.054 0.1144 3506 +3508 2 923.5649 1429.142 74.4934 0.1144 3507 +3509 2 922.9758 1428.1925 73.9332 0.1144 3508 +3510 2 922.3878 1427.243 73.3726 0.1144 3509 +3511 2 921.7997 1426.282 72.8123 0.1144 3510 +3512 2 921.2117 1425.3325 72.2518 0.1144 3511 +3513 2 920.6226 1424.3715 71.6912 0.1144 3512 +3514 2 920.0345 1423.422 71.1309 0.1144 3513 +3515 2 919.4465 1422.4725 70.5704 0.1144 3514 +3516 2 918.8585 1421.5115 70.0101 0.1144 3515 +3517 2 918.2705 1420.562 69.4495 0.1144 3516 +3518 2 917.6813 1419.601 68.8892 0.1144 3517 +3519 2 917.5852 1418.6515 67.3904 0.1144 3518 +3520 2 917.496 1417.6906 65.8764 0.1144 3519 +3521 2 917.4079 1416.7296 64.3622 0.1144 3520 +3522 2 917.3198 1415.7686 62.8477 0.1144 3521 +3523 2 917.2306 1414.8191 61.3334 0.1144 3522 +3524 2 917.1425 1413.8582 59.8195 0.1144 3523 +3525 2 917.0533 1412.8972 58.305 0.1144 3524 +3526 2 916.7558 1411.9362 57.2664 0.1144 3525 +3527 2 916.1461 1410.9867 56.9349 0.1144 3526 +3528 2 915.5363 1410.0258 56.6031 0.1144 3527 +3529 2 914.9266 1409.0648 56.2716 0.1144 3528 +3530 2 914.318 1408.1038 55.9398 0.1144 3529 +3531 2 913.7082 1407.1429 55.608 0.1144 3530 +3532 2 913.0985 1406.1934 55.2765 0.1144 3531 +3533 2 912.5024 1405.2896 54.4824 0.1144 3532 +3534 2 911.9224 1404.4545 53.188 0.1144 3533 +3535 2 911.3424 1403.6308 51.8935 0.1144 3534 +3536 2 910.7613 1402.7957 50.5991 0.1144 3535 +3537 2 910.5908 1401.7203 49.775 0.1144 3536 +3538 2 910.4513 1400.6335 48.9852 0.1144 3537 +3539 2 910.3105 1399.5467 48.195 0.1144 3538 +3540 2 910.1698 1398.4599 47.4048 0.1144 3539 +3541 2 910.0291 1397.3731 46.615 0.1144 3540 +3542 2 909.9239 1396.2978 45.7598 0.1144 3541 +3543 2 910.0703 1395.291 44.4326 0.1144 3542 +3544 2 910.2179 1394.2958 43.1052 0.1144 3543 +3545 2 910.3643 1393.3005 41.7777 0.1144 3544 +3546 2 910.5119 1392.3052 40.4505 0.1144 3545 +3547 2 910.6583 1391.3099 39.123 0.1144 3546 +3548 2 910.8482 1390.2346 38.3337 0.1144 3547 +3549 2 911.0404 1389.1478 37.5838 0.1144 3548 +3550 2 911.2326 1388.061 36.834 0.1144 3549 +3551 2 911.4248 1386.9742 36.0842 0.1144 3550 +3552 2 911.617 1385.8874 35.3343 0.1144 3551 +3553 2 911.8103 1384.8006 34.5848 0.1144 3552 +3554 2 911.8481 1383.7481 33.5199 0.1144 3553 +3555 2 911.8504 1382.707 32.3803 0.1144 3554 +3556 2 912.2279 1381.769 31.1774 0.1144 3555 +3557 2 912.7038 1380.8538 29.9583 0.1144 3556 +3558 2 913.1797 1379.9386 28.7392 0.1144 3557 +3559 2 913.6556 1379.0234 27.5201 0.1144 3558 +3560 2 914.1315 1378.1082 26.301 0.1144 3559 +3561 2 914.4415 1377.2044 25.1734 0.1144 3560 +3562 2 913.7574 1376.3121 24.5963 0.1144 3561 +3563 2 913.0733 1375.4312 24.019 0.1144 3562 +3564 2 912.388 1374.5503 23.4419 0.1144 3563 +3565 2 911.6925 1373.6694 22.96 0.1144 3564 +3566 2 910.9352 1372.8114 22.96 0.1144 3565 +3567 2 910.1778 1371.9534 22.96 0.1144 3566 +3568 2 909.4205 1371.0954 22.96 0.1144 3567 +3569 2 908.6632 1370.2374 22.96 0.1144 3568 +3570 2 907.9059 1369.3794 22.96 0.1144 3569 +3571 2 907.1485 1368.5214 22.96 0.1144 3570 +3572 2 906.3923 1367.6634 22.96 0.1144 3571 +3573 2 905.635 1366.8054 22.96 0.1144 3572 +3574 2 904.8777 1365.9474 22.96 0.1144 3573 +3575 2 904.1204 1365.0894 22.96 0.1144 3574 +3576 2 902.9192 1364.5518 22.6738 0.1144 3575 +3577 2 901.877 1364.0942 22.4258 0.1144 3576 +3578 2 900.8359 1363.6251 22.1777 0.1144 3577 +3579 2 899.7949 1363.1675 21.9296 0.1144 3578 +3580 2 898.7527 1362.7099 21.6815 0.1144 3579 +3581 2 897.7117 1362.2409 21.4334 0.1144 3580 +3582 2 896.6706 1361.7833 21.1854 0.1144 3581 +3583 2 895.6296 1361.3142 20.9376 0.1144 3582 +3584 2 894.5874 1360.8566 20.6895 0.1144 3583 +3585 2 893.5464 1360.3876 20.4414 0.1144 3584 +3586 2 892.5053 1359.93 20.1933 0.1144 3585 +3587 2 891.4631 1359.461 19.9452 0.1144 3586 +3588 2 890.4221 1359.0034 19.6972 0.1144 3587 +3589 2 889.3811 1358.5458 19.4494 0.1144 3588 +3590 2 888.34 1358.0767 19.2013 0.1144 3589 +3591 2 887.2978 1357.6191 18.9532 0.1144 3590 +3592 2 886.2568 1357.1501 18.7051 0.1144 3591 +3593 2 885.2158 1356.6925 18.457 0.1144 3592 +3594 2 884.1736 1356.2234 18.209 0.1144 3593 +3595 2 883.1325 1355.7658 17.9609 0.1144 3594 +3596 2 882.0915 1355.3082 17.7128 0.1144 3595 +3597 2 881.0505 1354.8392 17.465 0.1144 3596 +3598 2 880.0083 1354.3816 17.2169 0.1144 3597 +3599 2 878.9672 1353.9126 16.9688 0.1144 3598 +3600 2 877.9262 1353.455 16.7208 0.1144 3599 +3601 2 876.8852 1352.9859 16.4727 0.1144 3600 +3602 2 875.843 1352.5283 16.2246 0.1144 3601 +3603 2 874.8866 1351.9334 16.037 0.1144 3602 +3604 2 874.0675 1351.1441 15.9457 0.1144 3603 +3605 2 873.2472 1350.3433 15.8544 0.1144 3604 +3606 2 872.427 1349.5425 15.7632 0.1144 3605 +3607 2 871.6079 1348.7531 15.6719 0.1144 3606 +3608 2 870.7876 1347.9523 15.5806 0.1144 3607 +3609 2 869.9674 1347.1515 15.4893 0.1144 3608 +3610 2 869.1483 1346.3622 15.3983 0.1144 3609 +3611 2 868.328 1345.5614 15.307 0.1144 3610 +3612 2 867.5078 1344.7606 15.2158 0.1144 3611 +3613 2 866.6875 1343.9712 15.1245 0.1144 3612 +3614 2 865.8684 1343.1704 15.0332 0.1144 3613 +3615 2 865.0482 1342.3696 14.9419 0.1144 3614 +3616 2 864.2279 1341.5802 14.8509 0.1144 3615 +3617 2 863.4088 1340.7794 14.7596 0.1144 3616 +3618 2 862.5886 1339.9786 14.6684 0.1144 3617 +3619 2 861.7683 1339.1893 14.5771 0.1144 3618 +3620 2 860.9492 1338.3885 14.4858 0.1144 3619 +3621 2 860.129 1337.5877 14.3945 0.1144 3620 +3622 2 859.3087 1336.7983 14.3032 0.1144 3621 +3623 2 858.4885 1335.9975 14.212 0.1144 3622 +3624 2 857.6694 1335.1967 14.121 0.1144 3623 +3625 2 856.8491 1334.4074 14.0297 0.1144 3624 +3626 2 856.0289 1333.6066 13.9384 0.1144 3625 +3627 2 855.2098 1332.8058 13.8471 0.1144 3626 +3628 2 854.5291 1331.9363 13.776 0.1144 3627 +3629 2 854.3403 1330.8152 13.776 0.1144 3628 +3630 2 854.1516 1329.6826 13.776 0.1144 3629 +3631 2 853.964 1328.5501 13.776 0.1144 3630 +3632 2 853.7752 1327.429 13.776 0.1144 3631 +3633 2 853.5864 1326.2964 13.776 0.1144 3632 +3634 2 853.3988 1325.1638 13.776 0.1144 3633 +3635 2 853.4354 1324.0427 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1289.3566 9.184 0.1144 3678 +3680 2 884.2342 1288.9105 9.184 0.1144 3679 +3681 2 885.289 1288.4758 9.184 0.1144 3680 +3682 2 886.3437 1288.0296 9.184 0.1144 3681 +3683 2 887.3985 1287.5834 9.184 0.1144 3682 +3684 2 888.4533 1287.1487 9.184 0.1144 3683 +3685 2 889.508 1286.7026 9.184 0.1144 3684 +3686 2 890.5628 1286.2564 9.184 0.1144 3685 +3687 2 891.6187 1285.8217 9.184 0.1144 3686 +3688 2 892.6735 1285.3755 9.184 0.1144 3687 +3689 2 893.7283 1284.9294 9.184 0.1144 3688 +3690 2 894.783 1284.4832 9.184 0.1144 3689 +3691 2 895.8378 1284.0485 9.184 0.1144 3690 +3692 2 896.8926 1283.6023 9.184 0.1144 3691 +3693 2 897.9473 1283.1562 9.184 0.1144 3692 +3694 2 899.0032 1282.7214 9.184 0.1144 3693 +3695 2 900.058 1282.2753 9.184 0.1144 3694 +3696 2 901.1128 1281.8291 9.184 0.1144 3695 +3697 2 902.1676 1281.3944 9.184 0.1144 3696 +3698 2 903.2223 1280.9482 9.184 0.1144 3697 +3699 2 904.2771 1280.5021 9.184 0.1144 3698 +3700 2 905.3319 1280.0674 9.184 0.1144 3699 +3701 2 906.3866 1279.6212 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989.7533 1478.2882 131.4866 0.1144 3788 +3790 2 990.8676 1478.0366 131.6885 0.1144 3789 +3791 2 991.9807 1477.7849 131.8901 0.1144 3790 +3792 2 993.0938 1477.5332 132.0917 0.1144 3791 +3793 2 994.2081 1477.2815 132.2936 0.1144 3792 +3794 2 995.3212 1477.0413 132.4952 0.1144 3793 +3795 2 996.4343 1476.7896 132.697 0.1144 3794 +3796 2 997.5486 1476.5379 132.8986 0.1144 3795 +3797 2 998.6617 1476.2862 133.1005 0.1144 3796 +3798 2 999.7748 1476.046 133.3021 0.1144 3797 +3799 2 1000.889 1475.7943 133.504 0.1144 3798 +3800 2 1002.0021 1475.5426 133.7056 0.1144 3799 +3801 2 1003.1153 1475.291 133.9072 0.1144 3800 +3802 2 1004.2284 1475.0393 134.1091 0.1144 3801 +3803 2 1005.3426 1474.799 134.3107 0.1144 3802 +3804 2 1006.4557 1474.5474 134.5126 0.1144 3803 +3805 2 1007.5688 1474.2957 134.7142 0.1144 3804 +3806 2 1008.6831 1474.044 134.916 0.1144 3805 +3807 2 1009.7962 1473.7923 135.1176 0.1144 3806 +3808 2 1010.9093 1473.5521 135.3195 0.1144 3807 +3809 2 1012.0167 1473.289 135.6152 0.1144 3808 +3810 2 1013.1081 1473.0258 136.1486 0.1144 3809 +3811 2 1014.1983 1472.7627 136.682 0.1144 3810 +3812 2 1015.2897 1472.4882 137.2154 0.1144 3811 +3813 2 1016.3799 1472.225 137.7491 0.1144 3812 +3814 2 1017.4713 1471.9619 138.2825 0.1144 3813 +3815 2 1018.5615 1471.6874 138.8159 0.1144 3814 +3816 2 1019.6529 1471.4242 139.3496 0.1144 3815 +3817 2 1020.7432 1471.1611 139.883 0.1144 3816 +3818 2 1021.8345 1470.8866 140.4164 0.1144 3817 +3819 2 1022.9248 1470.6234 140.9498 0.1144 3818 +3820 2 1024.0161 1470.3603 141.4834 0.1144 3819 +3821 2 1025.1064 1470.0858 142.0168 0.1144 3820 +3822 2 1026.1977 1469.8226 142.5502 0.1144 3821 +3823 2 1027.288 1469.5481 143.0836 0.1144 3822 +3824 2 1028.3794 1469.285 143.6173 0.1144 3823 +3825 2 1029.4696 1469.0218 144.1507 0.1144 3824 +3826 2 1030.5575 1468.7587 144.7169 0.1144 3825 +3827 2 1031.594 1468.507 145.7322 0.1144 3826 +3828 2 1032.6316 1468.2554 146.7477 0.1144 3827 +3829 2 1033.6681 1468.0151 147.7633 0.1144 3828 +3830 2 1034.7057 1467.7634 148.7786 0.1144 3829 +3831 2 1035.7433 1467.5232 149.7941 0.1144 3830 +3832 2 1036.8072 1467.3058 150.5336 0.1144 3831 +3833 2 1037.9443 1467.18 150.5336 0.1144 3832 +3834 2 1039.0803 1467.0427 150.5336 0.1144 3833 +3835 2 1040.2175 1466.9169 150.5336 0.1144 3834 +3836 2 1041.3535 1466.791 150.5336 0.1144 3835 +3837 2 1042.4906 1466.6652 150.5336 0.1144 3836 +3838 2 1043.6277 1466.5279 150.5336 0.1144 3837 +3839 2 1044.7637 1466.4021 150.5336 0.1144 3838 +3840 2 1045.9009 1466.2762 150.5336 0.1144 3839 +3841 2 1047.038 1466.1504 150.5336 0.1144 3840 +3842 2 1048.174 1466.0131 150.5336 0.1144 3841 +3843 2 1049.3111 1465.8873 150.5336 0.1144 3842 +3844 2 1050.4471 1465.7614 150.5336 0.1144 3843 +3845 2 1051.5842 1465.6242 150.5336 0.1144 3844 +3846 2 1052.7214 1465.4983 150.5336 0.1144 3845 +3847 2 1053.8574 1465.3725 150.5336 0.1144 3846 +3848 2 1054.9945 1465.2466 150.5336 0.1144 3847 +3849 2 1056.1316 1465.1094 150.5336 0.1144 3848 +3850 2 1057.2676 1464.9835 150.5336 0.1144 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1460.8079 153.5772 0.1144 3870 +3872 2 1081.946 1460.5334 153.7718 0.1144 3871 +3873 2 1083.0534 1460.2588 153.9667 0.1144 3872 +3874 2 1084.1619 1459.9957 154.1613 0.1144 3873 +3875 2 1085.2705 1459.7211 154.3562 0.1144 3874 +3876 2 1086.379 1459.4466 154.5508 0.1144 3875 +3877 2 1087.4875 1459.172 154.7456 0.1144 3876 +3878 2 1088.5961 1458.9089 154.9402 0.1144 3877 +3879 2 1089.7046 1458.6343 155.1351 0.1144 3878 +3880 2 1090.8132 1458.3598 155.33 0.1144 3879 +3881 2 1091.9217 1458.0852 155.5246 0.1144 3880 +3882 2 1093.0302 1457.8221 155.7195 0.1144 3881 +3883 2 1094.1388 1457.5475 155.9141 0.1144 3882 +3884 2 1095.2462 1457.273 156.109 0.1144 3883 +3885 2 1096.3547 1456.9984 156.3036 0.1144 3884 +3886 2 1097.4632 1456.7353 156.4984 0.1144 3885 +3887 2 1098.5718 1456.4607 156.693 0.1144 3886 +3888 2 1099.6803 1456.1862 156.8879 0.1144 3887 +3889 2 1100.7888 1455.9116 157.0825 0.1144 3888 +3890 2 1101.8974 1455.6485 157.2774 0.1144 3889 +3891 2 1102.9453 1455.2138 157.5641 0.1144 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499.0849 1361.1655 61.1411 0.1144 4362 +4364 2 498.1628 1360.502 60.9129 0.1144 4363 +4365 2 497.2396 1359.827 60.6847 0.1144 4364 +4366 2 496.3175 1359.1635 60.4565 0.1144 4365 +4367 2 495.3943 1358.4886 60.2283 0.1144 4366 +4368 2 494.4723 1357.8136 60.0004 0.1144 4367 +4369 2 493.5491 1357.1501 59.7722 0.1144 4368 +4370 2 492.627 1356.4751 59.544 0.1144 4369 +4371 2 491.7038 1355.8116 59.3158 0.1144 4370 +4372 2 490.7817 1355.1366 59.0878 0.1144 4371 +4373 2 489.8585 1354.4731 58.8596 0.1144 4372 +4374 2 488.9364 1353.7982 58.6314 0.1144 4373 +4375 2 488.0132 1353.1346 58.4032 0.1144 4374 +4376 2 487.09 1352.4597 58.1753 0.1144 4375 +4377 2 486.168 1351.7847 57.9471 0.1144 4376 +4378 2 485.2448 1351.1212 57.7189 0.1144 4377 +4379 2 484.3227 1350.4462 57.4907 0.1144 4378 +4380 2 483.3995 1349.7827 57.2628 0.1144 4379 +4381 2 482.4774 1349.1078 57.0346 0.1144 4380 +4382 2 481.5542 1348.4442 56.8064 0.1144 4381 +4383 2 480.6322 1347.7693 56.5782 0.1144 4382 +4384 2 479.7089 1347.1058 56.3503 0.1144 4383 +4385 2 478.7857 1346.4308 56.1221 0.1144 4384 +4386 2 477.8637 1345.7558 55.8939 0.1144 4385 +4387 2 476.9405 1345.0923 55.6657 0.1144 4386 +4388 2 476.0184 1344.4174 55.4378 0.1144 4387 +4389 2 475.0952 1343.7538 55.2096 0.1144 4388 +4390 2 474.1731 1343.0789 54.9814 0.1144 4389 +4391 2 473.2499 1342.4154 54.7532 0.1144 4390 +4392 2 472.3279 1341.7404 54.5252 0.1144 4391 +4393 2 471.4046 1341.0769 54.297 0.1144 4392 +4394 2 470.4826 1340.4019 54.0688 0.1144 4393 +4395 2 469.5594 1339.727 53.8406 0.1144 4394 +4396 2 468.6373 1339.0634 53.6124 0.1144 4395 +4397 2 467.7141 1338.3885 53.3845 0.1144 4396 +4398 2 466.8035 1337.725 53.3397 0.1144 4397 +4399 2 465.9535 1337.0386 54.1638 0.1144 4398 +4400 2 465.1024 1336.3407 54.9881 0.1144 4399 +4401 2 464.2524 1335.6543 55.8121 0.1144 4400 +4402 2 463.4012 1334.9679 56.6364 0.1144 4401 +4403 2 462.5501 1334.2815 57.4605 0.1144 4402 +4404 2 461.7001 1333.5951 58.2845 0.1144 4403 +4405 2 460.849 1332.9087 59.1088 0.1144 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438.3797 1323.7453 71.6176 0.1144 4426 +4428 2 437.2929 1323.4707 72.1664 0.1144 4427 +4429 2 436.2061 1323.1962 72.7152 0.1144 4428 +4430 2 435.1193 1322.9102 73.264 0.1144 4429 +4431 2 434.0313 1322.6356 73.8128 0.1144 4430 +4432 2 432.9445 1322.361 74.3616 0.1144 4431 +4433 2 431.8371 1322.2123 74.7211 0.1144 4432 +4434 2 430.6954 1322.2924 74.774 0.1144 4433 +4435 2 429.5537 1322.361 74.8269 0.1144 4434 +4436 2 428.412 1322.4297 74.8798 0.1144 4435 +4437 2 427.2714 1322.4983 74.9328 0.1144 4436 +4438 2 426.1297 1322.5784 74.9857 0.1144 4437 +4439 2 424.988 1322.647 75.0386 0.1144 4438 +4440 2 423.8463 1322.7157 75.0915 0.1144 4439 +4441 2 422.7046 1322.7958 75.1447 0.1144 4440 +4442 2 421.5629 1322.8644 75.1976 0.1144 4441 +4443 2 420.4211 1322.933 75.2506 0.1144 4442 +4444 2 419.2806 1323.0017 75.3035 0.1144 4443 +4445 2 418.1389 1323.0818 75.3564 0.1144 4444 +4446 2 416.9972 1323.1504 75.4093 0.1144 4445 +4447 2 415.8554 1323.219 75.4622 0.1144 4446 +4448 2 414.7137 1323.2877 75.5152 0.1144 4447 +4449 2 413.572 1323.3678 75.5681 0.1144 4448 +4450 2 412.4303 1323.4364 75.621 0.1144 4449 +4451 2 411.2897 1323.505 75.6739 0.1144 4450 +4452 2 410.148 1323.5737 75.7268 0.1144 4451 +4453 2 409.0063 1323.6538 75.7798 0.1144 4452 +4454 2 407.8646 1323.7224 75.8327 0.1144 4453 +4455 2 536.981 1451.1526 48.8754 0.1144 4121 +4456 2 536.1985 1450.3174 49.1448 0.1144 4455 +4457 2 535.416 1449.4938 49.4138 0.1144 4456 +4458 2 534.6347 1448.6586 49.6832 0.1144 4457 +4459 2 533.93 1447.7778 49.9405 0.1144 4458 +4460 2 533.2859 1446.8282 50.1883 0.1144 4459 +4461 2 532.6407 1445.8902 50.4364 0.1144 4460 +4462 2 531.9966 1444.9521 50.6845 0.1144 4461 +4463 2 531.3525 1444.014 50.9323 0.1144 4462 +4464 2 530.403 1443.911 51.632 0.1144 4463 +4465 2 529.3139 1443.7966 52.4356 0.1144 4464 +4466 2 528.258 1443.6365 53.2098 0.1144 4465 +4467 2 527.8073 1442.5954 53.4654 0.1144 4466 +4468 2 527.3565 1441.543 53.7208 0.1144 4467 +4469 2 526.9058 1440.5019 53.9764 0.1144 4468 +4470 2 526.4551 1439.4494 54.2321 0.1144 4469 +4471 2 525.9448 1438.4427 54.5927 0.1144 4470 +4472 2 525.4014 1437.4474 55.0164 0.1144 4471 +4473 2 524.8569 1436.4636 55.44 0.1144 4472 +4474 2 524.3124 1435.4683 55.8636 0.1144 4473 +4475 2 523.7678 1434.473 56.2873 0.1144 4474 +4476 2 523.2473 1433.4778 56.7515 0.1144 4475 +4477 2 522.7874 1432.4596 57.3205 0.1144 4476 +4478 2 522.3275 1431.43 57.8892 0.1144 4477 +4479 2 521.8676 1430.4118 58.4578 0.1144 4478 +4480 2 521.4077 1429.3937 59.0265 0.1144 4479 +4481 2 520.9479 1428.3641 59.5952 0.1144 4480 +4482 2 520.4388 1427.3688 59.9869 0.1144 4481 +4483 2 519.8233 1426.4078 59.9869 0.1144 4482 +4484 2 519.2067 1425.4354 59.9869 0.1144 4483 +4485 2 518.5901 1424.4745 59.9869 0.1144 4484 +4486 2 517.9815 1423.5021 60.0104 0.1144 4485 +4487 2 517.517 1422.4839 60.4761 0.1144 4486 +4488 2 517.0525 1421.4543 60.9414 0.1144 4487 +4489 2 516.5869 1420.4247 61.4071 0.1144 4488 +4490 2 516.1225 1419.3951 61.8727 0.1144 4489 +4491 2 515.658 1418.3655 62.3381 0.1144 4490 +4492 2 515.1924 1417.3359 62.8037 0.1144 4491 +4493 2 514.7279 1416.3178 63.2694 0.1144 4492 +4494 2 514.2623 1415.2882 63.7347 0.1144 4493 +4495 2 513.7979 1414.2586 64.2004 0.1144 4494 +4496 2 513.3334 1413.229 64.666 0.1144 4495 +4497 2 512.8678 1412.1994 65.1316 0.1144 4496 +4498 2 512.4033 1411.1698 65.597 0.1144 4497 +4499 2 511.9377 1410.1402 66.0626 0.1144 4498 +4500 2 511.4732 1409.122 66.5283 0.1144 4499 +4501 2 511.0088 1408.0924 66.9936 0.1144 4500 +4502 2 510.5432 1407.0628 67.4593 0.1144 4501 +4503 2 510.0684 1406.0446 67.9098 0.1144 4502 +4504 2 509.2939 1405.1981 67.9098 0.1144 4503 +4505 2 508.5183 1404.363 67.9098 0.1144 4504 +4506 2 507.7438 1403.5164 67.9098 0.1144 4505 +4507 2 506.9682 1402.6698 67.9098 0.1144 4506 +4508 2 506.1937 1401.8347 67.9098 0.1144 4507 +4509 2 505.4181 1400.9882 67.9098 0.1144 4508 +4510 2 504.6436 1400.153 67.9098 0.1144 4509 +4511 2 503.8679 1399.3065 67.9098 0.1144 4510 +4512 2 503.122 1398.437 67.9098 0.1144 4511 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1378.1539 79.0961 0.1144 4533 +4535 2 488.6013 1377.193 79.3554 0.1144 4534 +4536 2 488.0167 1376.2091 79.5197 0.1144 4535 +4537 2 487.4321 1375.2253 79.6844 0.1144 4536 +4538 2 486.8475 1374.2529 79.8487 0.1144 4537 +4539 2 486.2629 1373.269 80.0134 0.1144 4538 +4540 2 485.6772 1372.2852 80.1777 0.1144 4539 +4541 2 485.0926 1371.3014 80.3421 0.1144 4540 +4542 2 484.7094 1370.2374 80.5706 0.1144 4541 +4543 2 484.3513 1369.1506 80.8069 0.1144 4542 +4544 2 483.9932 1368.0638 81.0432 0.1144 4543 +4545 2 483.634 1366.9885 81.2795 0.1144 4544 +4546 2 483.2759 1365.9017 81.5156 0.1144 4545 +4547 2 482.9167 1364.8263 81.7519 0.1144 4546 +4548 2 482.5586 1363.7395 81.9882 0.1144 4547 +4549 2 482.1994 1362.6642 82.2242 0.1144 4548 +4550 2 481.8414 1361.5774 82.4606 0.1144 4549 +4551 2 481.4821 1360.4906 82.6969 0.1144 4550 +4552 2 481.1241 1359.4152 82.9332 0.1144 4551 +4553 2 480.7649 1358.3284 83.1692 0.1144 4552 +4554 2 480.4068 1357.253 83.4056 0.1144 4553 +4555 2 480.0476 1356.1662 83.6419 0.1144 4554 +4556 2 479.6895 1355.0794 83.8779 0.1144 4555 +4557 2 479.3314 1354.0041 84.1142 0.1144 4556 +4558 2 478.9722 1352.9173 84.3506 0.1144 4557 +4559 2 478.6141 1351.8419 84.5866 0.1144 4558 +4560 2 478.2549 1350.7551 84.8229 0.1144 4559 +4561 2 477.8968 1349.6798 85.0592 0.1144 4560 +4562 2 477.5376 1348.593 85.2956 0.1144 4561 +4563 2 477.1796 1347.5062 85.5316 0.1144 4562 +4564 2 476.8203 1346.4308 85.7679 0.1144 4563 +4565 2 476.4623 1345.344 86.0042 0.1144 4564 +4566 2 476.1031 1344.2686 86.2403 0.1144 4565 +4567 2 475.745 1343.1818 86.4766 0.1144 4566 +4568 2 475.3869 1342.095 86.7129 0.1144 4567 +4569 2 475.0277 1341.0197 86.9492 0.1144 4568 +4570 2 474.6605 1339.9443 87.213 0.1144 4569 +4571 2 474.2383 1338.8918 87.6406 0.1144 4570 +4572 2 473.8173 1337.8394 88.0684 0.1144 4571 +4573 2 473.3964 1336.7983 88.496 0.1144 4572 +4574 2 472.9742 1335.7458 88.9235 0.1144 4573 +4575 2 472.5532 1334.6934 89.3511 0.1144 4574 +4576 2 472.1322 1333.6409 89.7786 0.1144 4575 +4577 2 471.7101 1332.5998 90.2062 0.1144 4576 +4578 2 471.2891 1331.5474 90.6338 0.1144 4577 +4579 2 470.8681 1330.4949 91.0613 0.1144 4578 +4580 2 470.446 1329.4538 91.4889 0.1144 4579 +4581 2 470.1119 1328.3899 92.1351 0.1144 4580 +4582 2 469.8465 1327.3374 92.9552 0.1144 4581 +4583 2 469.58 1326.2735 93.7754 0.1144 4582 +4584 2 469.3146 1325.2096 94.5955 0.1144 4583 +4585 2 469.0492 1324.1457 95.4156 0.1144 4584 +4586 2 468.7837 1323.0932 96.236 0.1144 4585 +4587 2 468.5183 1322.0293 97.0561 0.1144 4586 +4588 2 468.2209 1320.9425 97.2037 0.1144 4587 +4589 2 467.9063 1319.8442 96.9996 0.1144 4588 +4590 2 467.5928 1318.746 96.7957 0.1144 4589 +4591 2 467.2782 1317.6478 96.5916 0.1144 4590 +4592 2 466.9648 1316.5495 96.3878 0.1144 4591 +4593 2 466.6731 1315.4513 96.2475 0.1144 4592 +4594 2 466.5644 1314.3302 96.6316 0.1144 4593 +4595 2 466.4568 1313.1976 97.0155 0.1144 4594 +4596 2 466.3482 1312.0765 97.3997 0.1144 4595 +4597 2 466.2406 1310.9439 97.7838 0.1144 4596 +4598 2 466.132 1309.8114 98.168 0.1144 4597 +4599 2 465.9912 1308.6902 98.5902 0.1144 4598 +4600 2 465.7281 1307.6034 99.1508 0.1144 4599 +4601 2 465.4661 1306.5166 99.7116 0.1144 4600 +4602 2 465.2042 1305.4298 100.2722 0.1144 4601 +4603 2 464.9422 1304.3316 100.8328 0.1144 4602 +4604 2 464.6802 1303.2448 101.3933 0.1144 4603 +4605 2 464.4285 1302.1466 101.8805 0.1144 4604 +4606 2 464.2375 1301.0254 101.9802 0.1144 4605 +4607 2 464.0464 1299.8929 102.0799 0.1144 4606 +4608 2 463.8542 1298.7718 102.1793 0.1144 4607 +4609 2 463.6632 1297.6392 102.279 0.1144 4608 +4610 2 463.471 1296.5181 102.3784 0.1144 4609 +4611 2 463.28 1295.3855 102.478 0.1144 4610 +4612 2 463.0889 1294.2644 102.5774 0.1144 4611 +4613 2 462.8967 1293.1318 102.6771 0.1144 4612 +4614 2 462.7057 1292.0107 102.7768 0.1144 4613 +4615 2 462.5135 1290.8782 102.8762 0.1144 4614 +4616 2 462.3224 1289.757 102.9759 0.1144 4615 +4617 2 461.8488 1288.7389 103.3684 0.1144 4616 +4618 2 461.2997 1287.755 103.8386 0.1144 4617 +4619 2 460.7506 1286.7712 104.309 0.1144 4618 +4620 2 460.2026 1285.7759 104.7791 0.1144 4619 +4621 2 459.6535 1284.7921 105.2492 0.1144 4620 +4622 2 459.1044 1283.8082 105.7193 0.1144 4621 +4623 2 458.5564 1282.8244 106.1894 0.1144 4622 +4624 2 458.1571 1281.7834 106.6968 0.1144 4623 +4625 2 457.8711 1280.6966 107.2319 0.1144 4624 +4626 2 457.5863 1279.6098 107.7672 0.1144 4625 +4627 2 457.3003 1278.523 108.3026 0.1144 4626 +4628 2 457.0143 1277.4362 108.838 0.1144 4627 +4629 2 456.7294 1276.3494 109.3733 0.1144 4628 +4630 2 456.4434 1275.2626 109.9084 0.1144 4629 +4631 2 456.1574 1274.1758 110.4438 0.1144 4630 +4632 2 455.8726 1273.1004 110.9791 0.1144 4631 +4633 2 455.5866 1272.0136 111.5145 0.1144 4632 +4634 2 455.3006 1270.9268 112.0498 0.1144 4633 +4635 2 455.0157 1269.84 112.5849 0.1144 4634 +4636 2 454.7297 1268.7532 113.1203 0.1144 4635 +4637 2 454.4448 1267.6664 113.6556 0.1144 4636 +4638 2 454.1588 1266.5796 114.191 0.1144 4637 +4639 2 454.3899 1265.5157 113.9933 0.1144 4638 +4640 2 454.7778 1264.4518 113.5744 0.1144 4639 +4641 2 455.1656 1263.3878 113.1556 0.1144 4640 +4642 2 455.5534 1262.3239 112.7367 0.1144 4641 +4643 2 455.9412 1261.26 112.3178 0.1144 4642 +4644 2 456.329 1260.1961 111.8992 0.1144 4643 +4645 2 456.7168 1259.1322 111.4803 0.1144 4644 +4646 2 457.1046 1258.0682 111.0614 0.1144 4645 +4647 2 457.4925 1257.0158 110.6426 0.1144 4646 +4648 2 457.8803 1255.9518 110.2237 0.1144 4647 +4649 2 458.2681 1254.8879 109.8048 0.1144 4648 +4650 2 458.9179 1253.9727 110.3172 0.1144 4649 +4651 2 459.5791 1253.069 110.8691 0.1144 4650 +4652 2 460.2404 1252.1652 111.4212 0.1144 4651 +4653 2 460.9016 1251.2614 111.9734 0.1144 4652 +4654 2 461.5628 1250.3462 112.5256 0.1144 4653 +4655 2 462.224 1249.4425 113.0774 0.1144 4654 +4656 2 462.8853 1248.5387 113.6296 0.1144 4655 +4657 2 463.5465 1247.635 114.1818 0.1144 4656 +4658 2 464.2077 1246.7312 114.7336 0.1144 4657 +4659 2 464.8701 1245.816 115.2858 0.1144 4658 +4660 2 465.5314 1244.9122 115.838 0.1144 4659 +4661 2 466.1926 1244.0085 116.3901 0.1144 4660 +4662 2 466.5758 1243.0361 115.8018 0.1144 4661 +4663 2 466.8149 1242.0294 114.6222 0.1144 4662 +4664 2 467.0552 1241.0112 113.4426 0.1144 4663 +4665 2 467.2943 1240.0045 112.2632 0.1144 4664 +4666 2 467.5345 1238.9978 111.0836 0.1144 4665 +4667 2 467.7736 1237.991 109.9039 0.1144 4666 +4668 2 468.1671 1236.9386 110.1024 0.1144 4667 +4669 2 468.5778 1235.8746 110.4519 0.1144 4668 +4670 2 468.9885 1234.8222 110.801 0.1144 4669 +4671 2 469.3981 1233.7582 111.1502 0.1144 4670 +4672 2 469.8088 1232.7058 111.4996 0.1144 4671 +4673 2 470.2183 1231.6418 111.8488 0.1144 4672 +4674 2 470.629 1230.5894 112.198 0.1144 4673 +4675 2 471.0397 1229.5254 112.5474 0.1144 4674 +4676 2 471.4493 1228.473 112.8966 0.1144 4675 +4677 2 471.86 1227.409 113.2457 0.1144 4676 +4678 2 472.2707 1226.3451 113.5952 0.1144 4677 +4679 2 472.6802 1225.2926 113.9443 0.1144 4678 +4680 2 473.0909 1224.2287 114.2935 0.1144 4679 +4681 2 473.5005 1223.1762 114.6429 0.1144 4680 +4682 2 473.9112 1222.1123 114.9921 0.1144 4681 +4683 2 474.3218 1221.0598 115.3412 0.1144 4682 +4684 2 474.9499 1220.1332 115.7442 0.1144 4683 +4685 2 475.6729 1219.2638 116.1706 0.1144 4684 +4686 2 476.3971 1218.3943 116.5968 0.1144 4685 +4687 2 477.1201 1217.5249 117.0229 0.1144 4686 +4688 2 477.8431 1216.6554 117.4491 0.1144 4687 +4689 2 478.5661 1215.786 117.8752 0.1144 4688 +4690 2 479.2891 1214.9166 118.3014 0.1144 4689 +4691 2 480.0132 1214.0471 118.7276 0.1144 4690 +4692 2 480.7363 1213.1777 119.154 0.1144 4691 +4693 2 481.4593 1212.3082 119.5802 0.1144 4692 +4694 2 482.1823 1211.4388 120.0063 0.1144 4693 +4695 2 482.9053 1210.5694 120.4325 0.1144 4694 +4696 2 483.6294 1209.6999 120.8586 0.1144 4695 +4697 2 484.3524 1208.8305 121.2848 0.1144 4696 +4698 2 485.0754 1207.961 121.7112 0.1144 4697 +4699 2 485.7985 1207.0916 122.1374 0.1144 4698 +4700 2 486.5215 1206.2222 122.5636 0.1144 4699 +4701 2 487.2456 1205.3527 122.9897 0.1144 4700 +4702 2 487.9686 1204.4947 123.4159 0.1144 4701 +4703 2 488.6916 1203.6253 123.842 0.1144 4702 +4704 2 489.4146 1202.7558 124.2682 0.1144 4703 +4705 2 490.0816 1201.8635 124.8629 0.1144 4704 +4706 2 490.6799 1200.9369 125.6604 0.1144 4705 +4707 2 491.2782 1200.0217 126.4581 0.1144 4706 +4708 2 491.8765 1199.1065 127.2555 0.1144 4707 +4709 2 492.4748 1198.1798 128.0532 0.1144 4708 +4710 2 493.072 1197.2646 128.851 0.1144 4709 +4711 2 493.9209 1197.2532 130.2311 0.1144 4710 +4712 2 494.8418 1197.5049 131.7786 0.1144 4711 +4713 2 495.7616 1197.7451 133.3259 0.1144 4712 +4714 2 496.6825 1197.9968 134.8735 0.1144 4713 +4715 2 497.6022 1198.2485 136.4208 0.1144 4714 +4716 2 498.5232 1198.5002 137.9683 0.1144 4715 +4717 2 499.4429 1198.7404 139.5159 0.1144 4716 +4718 2 500.3639 1198.9921 141.0632 0.1144 4717 +4719 2 501.2836 1199.2438 142.6107 0.1144 4718 +4720 2 531.261 1443.7166 50.8346 0.1144 4463 +4721 2 530.9281 1442.6298 50.4815 0.1144 4720 +4722 2 530.5964 1441.543 50.1284 0.1144 4721 +4723 2 530.2646 1440.4562 49.775 0.1144 4722 +4724 2 529.9317 1439.3694 49.422 0.1144 4723 +4725 2 529.5999 1438.2826 49.0689 0.1144 4724 +4726 2 529.2682 1437.2072 48.7158 0.1144 4725 +4727 2 528.9364 1436.1204 48.3624 0.1144 4726 +4728 2 528.6035 1435.0336 48.0094 0.1144 4727 +4729 2 528.2717 1433.9468 47.6563 0.1144 4728 +4730 2 527.9823 1432.8714 47.091 0.1144 4729 +4731 2 527.7146 1431.7846 46.4176 0.1144 4730 +4732 2 527.4469 1430.7093 45.7442 0.1144 4731 +4733 2 527.1792 1429.6339 45.0708 0.1144 4732 +4734 2 526.9115 1428.5586 44.3974 0.1144 4733 +4735 2 526.6438 1427.4832 43.724 0.1144 4734 +4736 2 526.3761 1426.3964 43.0506 0.1144 4735 +4737 2 526.1084 1425.321 42.3772 0.1144 4736 +4738 2 525.8407 1424.2457 41.7038 0.1144 4737 +4739 2 525.573 1423.1703 41.0304 0.1144 4738 +4740 2 525.1498 1422.1636 40.2363 0.1144 4739 +4741 2 524.6132 1421.2255 39.3537 0.1144 4740 +4742 2 524.0767 1420.276 38.4712 0.1144 4741 +4743 2 523.5402 1419.3379 37.5886 0.1144 4742 +4744 2 523.0036 1418.3884 36.706 0.1144 4743 +4745 2 522.4671 1417.4503 35.8235 0.1144 4744 +4746 2 521.9717 1416.4779 35.0006 0.1144 4745 +4747 2 521.6915 1415.3911 34.4784 0.1144 4746 +4748 2 521.4112 1414.3043 33.9562 0.1144 4747 +4749 2 521.1309 1413.2175 33.4342 0.1144 4748 +4750 2 520.8506 1412.1307 32.912 0.1144 4749 +4751 2 520.5703 1411.0439 32.3898 0.1144 4750 +4752 2 520.2901 1409.9457 31.8679 0.1144 4751 +4753 2 520.0098 1408.8589 31.3457 0.1144 4752 +4754 2 519.7295 1407.7721 30.8238 0.1144 4753 +4755 2 519.6368 1406.7082 29.9765 0.1144 4754 +4756 2 519.7364 1405.6786 28.7969 0.1144 4755 +4757 2 519.8347 1404.649 27.617 0.1144 4756 +4758 2 519.9343 1403.6194 26.4373 0.1144 4757 +4759 2 520.0338 1402.5783 25.2574 0.1144 4758 +4760 2 519.9045 1401.5144 24.3992 0.1144 4759 +4761 2 519.678 1400.4276 23.6807 0.1144 4760 +4762 2 519.4504 1399.3522 22.9622 0.1144 4761 +4763 2 519.1941 1398.2883 22.1376 0.1144 4762 +4764 2 518.9138 1397.2473 21.2248 0.1144 4763 +4765 2 518.6335 1396.1948 20.3123 0.1144 4764 +4766 2 518.3533 1395.1538 19.3998 0.1144 4765 +4767 2 518.073 1394.1127 18.4873 0.1144 4766 +4768 2 517.7927 1393.0717 17.5748 0.1144 4767 +4769 2 517.5124 1392.0192 16.6622 0.1144 4768 +4770 2 517.2321 1390.9782 15.7497 0.1144 4769 +4771 2 516.9519 1389.9371 14.8369 0.1144 4770 +4772 2 516.6716 1388.8846 13.9244 0.1144 4771 +4773 2 516.1888 1387.8894 13.4753 0.1144 4772 +4774 2 515.5848 1386.917 13.3045 0.1144 4773 +4775 2 514.9796 1385.956 13.1337 0.1144 4774 +4776 2 514.3756 1384.9836 12.9626 0.1144 4775 +4777 2 513.7715 1384.0112 12.7918 0.1144 4776 +4778 2 513.1664 1383.0502 12.621 0.1144 4777 +4779 2 512.5623 1382.0778 12.4502 0.1144 4778 +4780 2 594.483 1566.8796 28.3802 0.1144 3998 +4781 2 594.8994 1567.9435 28.6703 0.1144 4780 +4782 2 595.3159 1568.996 28.9607 0.1144 4781 +4783 2 595.7311 1570.0599 29.251 0.1144 4782 +4784 2 596.3455 1570.9637 29.3384 0.1144 4783 +4785 2 597.2687 1571.6272 29.1096 0.1144 4784 +4786 2 598.1907 1572.3022 28.8812 0.1144 4785 +4787 2 599.1139 1572.9771 28.6524 0.1144 4786 +4788 2 600.036 1573.6406 28.4239 0.1144 4787 +4789 2 601.0187 1574.1783 28.5401 0.1144 4788 +4790 2 602.078 1574.5444 29.0962 0.1144 4789 +4791 2 603.1374 1574.9219 29.6523 0.1144 4790 +4792 2 604.1956 1575.288 30.2084 0.1144 4791 +4793 2 605.2847 1575.5397 30.6233 0.1144 4792 +4794 2 606.4252 1575.6083 30.7972 0.1144 4793 +4795 2 607.5647 1575.6655 30.9708 0.1144 4794 +4796 2 608.7041 1575.7342 31.1444 0.1144 4795 +4797 2 609.8447 1575.8028 31.318 0.1144 4796 +4798 2 610.9841 1575.86 31.4916 0.1144 4797 +4799 2 612.1247 1575.9286 31.6652 0.1144 4798 +4800 2 613.0753 1576.5006 31.269 0.1144 4799 +4801 2 613.9928 1577.1527 30.7723 0.1144 4800 +4802 2 614.8668 1577.7476 31.3152 0.1144 4801 +4803 2 615.71 1578.2967 32.6371 0.1144 4802 +4804 2 616.5531 1578.8458 33.9587 0.1144 4803 +4805 2 617.3962 1579.4064 35.2803 0.1144 4804 +4806 2 618.2393 1579.9555 36.6022 0.1144 4805 +4807 2 619.0825 1580.5161 37.9238 0.1144 4806 +4808 2 619.9256 1581.0652 39.2454 0.1144 4807 +4809 2 620.7676 1581.6143 40.5672 0.1144 4808 +4810 2 621.7354 1582.049 41.5999 0.1144 4809 +4811 2 622.7238 1582.4609 42.5877 0.1144 4810 +4812 2 623.7111 1582.8727 43.5753 0.1144 4811 +4813 2 624.6984 1583.296 44.5628 0.1144 4812 +4814 2 625.6856 1583.7078 45.5504 0.1144 4813 +4815 2 626.6821 1584.1197 46.4346 0.1144 4814 +4816 2 627.73 1584.5773 46.6558 0.1144 4815 +4817 2 628.7779 1585.0234 46.8768 0.1144 4816 +4818 2 629.8269 1585.4696 47.0977 0.1144 4817 +4819 2 630.8748 1585.9158 47.3189 0.1144 4818 +4820 2 631.9227 1586.3734 47.5432 0.1144 4819 +4821 2 632.8905 1586.9454 48.0396 0.1144 4820 +4822 2 633.8584 1587.5174 48.5358 0.1144 4821 +4823 2 634.8262 1588.0894 49.0322 0.1144 4822 +4824 2 635.794 1588.6728 49.5284 0.1144 4823 +4825 2 636.7618 1589.2448 50.0248 0.1144 4824 +4826 2 637.7297 1589.8168 50.521 0.1144 4825 +4827 2 638.7044 1590.3888 51.016 0.1144 4826 +4828 2 639.7111 1590.8922 51.5049 0.1144 4827 +4829 2 640.7189 1591.3955 51.9938 0.1144 4828 +4830 2 641.7268 1591.8989 52.4826 0.1144 4829 +4831 2 642.7335 1592.4022 52.9712 0.1144 4830 +4832 2 643.6659 1593.0314 53.3058 0.1144 4831 +4833 2 644.5342 1593.775 53.5094 0.1144 4832 +4834 2 645.4025 1594.5072 53.7127 0.1144 4833 +4835 2 646.2719 1595.2508 53.916 0.1144 4834 +4836 2 647.1402 1595.9944 54.1192 0.1144 4835 +4837 2 648.0085 1596.7266 54.3228 0.1144 4836 +4838 2 648.9088 1597.1956 55.6161 0.1144 4837 +4839 2 649.8103 1597.6418 56.9377 0.1144 4838 +4840 2 650.7129 1598.0994 58.2593 0.1144 4839 +4841 2 651.6144 1598.5455 59.5809 0.1144 4840 +4842 2 652.5159 1599.0031 60.9025 0.1144 4841 +4843 2 653.5523 1599.3578 61.6092 0.1144 4842 +4844 2 654.6162 1599.7124 62.1953 0.1144 4843 +4845 2 655.6802 1600.0556 62.7816 0.1144 4844 +4846 2 656.7441 1600.3988 63.3676 0.1144 4845 +4847 2 657.7794 1600.8678 63.7143 0.1144 4846 +4848 2 658.8136 1601.3369 64.0548 0.1144 4847 +4849 2 659.8478 1601.8059 64.3952 0.1144 4848 +4850 2 660.8831 1602.275 64.7354 0.1144 4849 +4851 2 661.9173 1602.7326 65.0759 0.1144 4850 +4852 2 662.9514 1603.2016 65.4164 0.1144 4851 +4853 2 663.9868 1603.6706 65.7569 0.1144 4852 +4854 2 665.0209 1604.1397 66.0971 0.1144 4853 +4855 2 666.0562 1604.6087 66.4376 0.1144 4854 +4856 2 667.0904 1605.0778 66.778 0.1144 4855 +4857 2 668.366 1605.215 66.4807 0.1144 4856 +4858 2 669.4974 1605.3523 66.2169 0.1144 4857 +4859 2 670.63 1605.4782 65.9532 0.1144 4858 +4860 2 671.7614 1605.604 65.6897 0.1144 4859 +4861 2 672.8939 1605.7298 65.4259 0.1144 4860 +4862 2 674.0254 1605.8557 65.1622 0.1144 4861 +4863 2 675.1568 1605.9815 64.8984 0.1144 4862 +4864 2 676.2893 1606.1074 64.6346 0.1144 4863 +4865 2 677.4265 1606.1874 64.5142 0.1144 4864 +4866 2 678.5705 1606.2218 64.5142 0.1144 4865 +4867 2 679.7133 1606.2675 64.5142 0.1144 4866 +4868 2 680.8081 1606.0959 64.3042 0.1144 4867 +4869 2 681.8332 1605.6383 63.8002 0.1144 4868 +4870 2 682.8593 1605.1693 63.2965 0.1144 4869 +4871 2 683.8843 1604.7117 62.7928 0.1144 4870 +4872 2 684.9094 1604.2426 62.2888 0.1144 4871 +4873 2 685.9355 1603.785 61.7851 0.1144 4872 +4874 2 686.9606 1603.316 61.2814 0.1144 4873 +4875 2 687.9867 1602.8584 60.7774 0.1144 4874 +4876 2 689.0118 1602.3894 60.2736 0.1144 4875 +4877 2 689.8778 1601.7258 59.9127 0.1144 4876 +4878 2 690.5344 1600.7878 59.7402 0.1144 4877 +4879 2 691.1911 1599.8497 59.5678 0.1144 4878 +4880 2 691.8477 1598.923 59.3953 0.1144 4879 +4881 2 692.5032 1597.985 59.2228 0.1144 4880 +4882 2 693.1599 1597.0469 59.0503 0.1144 4881 +4883 2 693.8166 1596.1202 58.8778 0.1144 4882 +4884 2 694.13 1595.022 58.8552 0.1144 4883 +4885 2 694.3897 1593.9123 58.8552 0.1144 4884 +4886 2 694.6505 1592.8026 58.8552 0.1144 4885 +4887 2 694.8873 1591.7845 58.7871 0.1144 4886 +4888 2 694.6494 1592.734 57.3488 0.1144 4887 +4889 2 694.4114 1593.6835 55.9104 0.1144 4888 +4890 2 694.1735 1594.6445 54.472 0.1144 4889 +4891 2 694.2787 1593.7178 54.3278 0.1144 4890 +4892 2 694.4229 1592.5853 54.3278 0.1144 4891 +4893 2 694.567 1591.4413 54.3278 0.1144 4892 +4894 2 693.8486 1590.8922 52.666 0.1144 4893 +4895 2 693.1187 1590.343 50.9816 0.1144 4894 +4896 2 692.9368 1589.9884 49.6059 0.1144 4895 +4897 2 694.0408 1590.1257 48.9552 0.1144 4896 +4898 2 695.1459 1590.2515 48.3045 0.1144 4897 +4899 2 696.251 1590.3888 47.6535 0.1144 4898 +4900 2 696.4832 1589.4965 47.0459 0.1144 4899 +4901 2 696.5244 1588.3754 46.4475 0.1144 4900 +4902 2 696.5656 1587.2657 45.8492 0.1144 4901 +4903 2 696.6079 1586.1446 45.2508 0.1144 4902 +4904 2 696.6491 1585.0234 44.6524 0.1144 4903 +4905 2 696.744 1583.9252 44.0787 0.1144 4904 +4906 2 697.1559 1582.8727 43.6481 0.1144 4905 +4907 2 697.5666 1581.8088 43.2177 0.1144 4906 +4908 2 697.9784 1580.7563 42.7871 0.1144 4907 +4909 2 698.3891 1579.7038 42.3567 0.1144 4908 +4910 2 698.801 1578.6514 41.9261 0.1144 4909 +4911 2 699.2117 1577.5989 41.4957 0.1144 4910 +4912 2 699.6224 1576.5464 41.0651 0.1144 4911 +4913 2 700.0822 1575.5168 40.6773 0.1144 4912 +4914 2 700.6794 1574.5444 40.4116 0.1144 4913 +4915 2 701.2754 1573.5834 40.1456 0.1144 4914 +4916 2 701.8726 1572.611 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1028.6779 1545.2351 36.2186 0.1144 5298 +5300 2 1028.2752 1544.1598 36.2186 0.1144 5299 +5301 2 1027.8337 1543.1187 36.078 0.1144 5300 +5302 2 1027.1793 1542.2607 35.1669 0.1144 5301 +5303 2 1026.5249 1541.4027 34.2555 0.1144 5302 +5304 2 1026.1028 1540.396 33.5026 0.1144 5303 +5305 2 1025.7962 1539.3321 32.8272 0.1144 5304 +5306 2 1025.4885 1538.2682 32.1521 0.1144 5305 +5307 2 1025.1819 1537.2042 31.4768 0.1144 5306 +5308 2 1024.8741 1536.1289 30.8017 0.1144 5307 +5309 2 1024.3891 1535.145 30.1409 0.1144 5308 +5310 2 1023.8045 1534.1955 29.4882 0.1144 5309 +5311 2 1023.2199 1533.246 28.8358 0.1144 5310 +5312 2 1022.6834 1532.285 28.131 0.1144 5311 +5313 2 1022.3768 1531.2554 27.1757 0.1144 5312 +5314 2 1022.069 1530.2258 26.2206 0.1144 5313 +5315 2 1021.7625 1529.1962 25.2652 0.1144 5314 +5316 2 1021.4559 1528.1666 24.3102 0.1144 5315 +5317 2 1021.2866 1527.1714 23.1014 0.1144 5316 +5318 2 1021.2957 1526.2104 21.5606 0.1144 5317 +5319 2 1021.0715 1525.5583 20.1415 0.1144 5318 +5320 2 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1549.1018 56.9058 0.1144 5574 +5576 2 686.1426 1547.9922 56.7347 0.1144 5575 +5577 2 685.9195 1546.8825 56.4959 0.1144 5576 +5578 2 685.9996 1545.7614 55.9118 0.1144 5577 +5579 2 686.0797 1544.6517 55.3274 0.1144 5578 +5580 2 686.1586 1543.5306 54.7434 0.1144 5579 +5581 2 686.1357 1542.4209 54.3278 0.1144 5580 +5582 2 685.86 1541.3112 54.3278 0.1144 5581 +5583 2 685.5843 1540.2015 54.3278 0.1144 5582 +5584 2 685.3967 1539.0918 54.028 0.1144 5583 +5585 2 685.3121 1537.9822 53.384 0.1144 5584 +5586 2 685.2263 1536.8725 52.74 0.1144 5585 +5587 2 685.1416 1535.7628 52.0957 0.1144 5586 +5588 2 685.0341 1534.6302 51.7535 0.1144 5587 +5589 2 684.9265 1533.4977 51.4268 0.1144 5588 +5590 2 684.8178 1532.3651 51.1 0.1144 5589 +5591 2 684.7103 1531.2326 50.7732 0.1144 5590 +5592 2 684.6028 1530.1 50.4465 0.1144 5591 +5593 2 684.4941 1528.9789 50.1197 0.1144 5592 +5594 2 684.39 1527.8463 49.8117 0.1144 5593 +5595 2 684.4358 1526.7138 50.3014 0.1144 5594 +5596 2 684.4804 1525.5926 50.7912 0.1144 5595 +5597 2 684.5261 1524.4715 51.2812 0.1144 5596 +5598 2 684.5719 1523.339 51.7709 0.1144 5597 +5599 2 684.6165 1522.2178 52.2606 0.1144 5598 +5600 2 684.6623 1521.0853 52.7503 0.1144 5599 +5601 2 684.6863 1519.9642 53.2098 0.1144 5600 +5602 2 684.4987 1518.843 53.3632 0.1144 5601 +5603 2 684.3099 1517.7105 53.5167 0.1144 5602 +5604 2 684.1223 1516.5894 53.6701 0.1144 5603 +5605 2 683.9335 1515.4568 53.8236 0.1144 5604 +5606 2 683.7459 1514.3357 53.977 0.1144 5605 +5607 2 683.5583 1513.2031 54.1304 0.1144 5606 +5608 2 683.3695 1512.082 54.2839 0.1144 5607 +5609 2 683.1819 1510.9494 54.4373 0.1144 5608 +5610 2 682.9932 1509.8283 54.5908 0.1144 5609 +5611 2 682.8056 1508.6958 54.7442 0.1144 5610 +5612 2 682.6168 1507.5746 54.8976 0.1144 5611 +5613 2 682.4292 1506.4421 55.0511 0.1144 5612 +5614 2 682.2416 1505.321 55.2045 0.1144 5613 +5615 2 682.0528 1504.1884 55.358 0.1144 5614 +5616 2 682.0231 1503.0787 55.5738 0.1144 5615 +5617 2 682.3068 1501.969 55.9124 0.1144 5616 +5618 2 682.5893 1500.8708 56.2512 0.1144 5617 +5619 2 682.873 1499.7726 56.5897 0.1144 5618 +5620 2 683.1568 1498.6743 56.9285 0.1144 5619 +5621 2 683.4393 1497.5761 57.267 0.1144 5620 +5622 2 683.723 1496.4778 57.6058 0.1144 5621 +5623 2 684.0056 1495.3796 57.9446 0.1144 5622 +5624 2 684.2893 1494.2814 58.2831 0.1144 5623 +5625 2 684.5719 1493.1717 58.6219 0.1144 5624 +5626 2 684.8556 1492.0734 58.9604 0.1144 5625 +5627 2 685.1382 1490.9752 59.2992 0.1144 5626 +5628 2 685.4219 1489.877 59.638 0.1144 5627 +5629 2 685.7044 1488.7787 59.9766 0.1144 5628 +5630 2 686.0797 1487.7034 59.7178 0.1144 5629 +5631 2 686.4572 1486.628 59.4406 0.1144 5630 +5632 2 686.8347 1485.5526 59.1632 0.1144 5631 +5633 2 687.2122 1484.4773 58.8857 0.1144 5632 +5634 2 687.4582 1483.3676 58.8552 0.1144 5633 +5635 2 687.6881 1482.2465 58.8552 0.1144 5634 +5636 2 687.9181 1481.1254 58.8552 0.1144 5635 +5637 2 688.148 1480.0042 58.8552 0.1144 5636 +5638 2 688.378 1478.8831 58.8552 0.1144 5637 +5639 2 687.941 1478.0022 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1434.1756 63.5578 0.1144 5681 +5683 2 670.551 1433.043 63.4253 0.1144 5682 +5684 2 670.6997 1431.9105 63.2932 0.1144 5683 +5685 2 670.8485 1430.7779 63.161 0.1144 5684 +5686 2 670.9983 1429.6454 63.0286 0.1144 5685 +5687 2 671.147 1428.5128 62.8964 0.1144 5686 +5688 2 671.2958 1427.3688 62.7642 0.1144 5687 +5689 2 671.4445 1426.2362 62.6318 0.1144 5688 +5690 2 671.5944 1425.1037 62.4996 0.1144 5689 +5691 2 671.7431 1423.9711 62.3675 0.1144 5690 +5692 2 671.9467 1422.8614 62.2507 0.1144 5691 +5693 2 672.5553 1421.889 62.2507 0.1144 5692 +5694 2 673.1651 1420.9281 62.2507 0.1144 5693 +5695 2 673.7737 1419.9557 62.2507 0.1144 5694 +5696 2 674.3823 1418.9833 62.2507 0.1144 5695 +5697 2 674.7198 1417.988 62.2507 0.1144 5696 +5698 2 674.3331 1416.9126 62.2507 0.1144 5697 +5699 2 673.9476 1415.8373 62.2507 0.1144 5698 +5700 2 673.5609 1414.7619 62.2507 0.1144 5699 +5701 2 673.1754 1413.6866 62.2507 0.1144 5700 +5702 2 672.7887 1412.6112 62.2507 0.1144 5701 +5703 2 672.0359 1411.7875 62.2507 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669.1382 1388.713 67.7099 0.1144 5724 +5726 2 669.2045 1387.5805 68.0226 0.1144 5725 +5727 2 669.2709 1386.4479 68.3357 0.1144 5726 +5728 2 669.3361 1385.3154 68.6484 0.1144 5727 +5729 2 669.4024 1384.1714 68.9615 0.1144 5728 +5730 2 669.0878 1383.096 69.0416 0.1144 5729 +5731 2 668.6428 1382.0435 69.0416 0.1144 5730 +5732 2 668.1978 1380.991 69.0416 0.1144 5731 +5733 2 667.7528 1379.9386 69.0416 0.1144 5732 +5734 2 667.3078 1378.8861 69.0416 0.1144 5733 +5735 2 666.8616 1377.8336 69.0416 0.1144 5734 +5736 2 666.4166 1376.7811 69.0416 0.1144 5735 +5737 2 665.9716 1375.7286 69.0416 0.1144 5736 +5738 2 665.9865 1374.6418 69.0416 0.1144 5737 +5739 2 666.3068 1373.5436 69.0416 0.1144 5738 +5740 2 666.626 1372.4454 69.0416 0.1144 5739 +5741 2 666.9463 1371.3471 69.0416 0.1144 5740 +5742 2 667.2655 1370.2489 69.0416 0.1144 5741 +5743 2 667.5858 1369.1506 69.0416 0.1144 5742 +5744 2 667.905 1368.0524 69.0416 0.1144 5743 +5745 2 668.2253 1366.9542 69.0416 0.1144 5744 +5746 2 668.5444 1365.8559 69.0416 0.1144 5745 +5747 2 668.8648 1364.7577 69.0416 0.1144 5746 +5748 2 669.1839 1363.6594 69.0416 0.1144 5747 +5749 2 669.5043 1362.5612 69.0416 0.1144 5748 +5750 2 669.8234 1361.463 69.0416 0.1144 5749 +5751 2 670.1438 1360.3647 69.0416 0.1144 5750 +5752 2 669.7674 1359.3122 69.0416 0.1144 5751 +5753 2 669.3109 1358.2598 69.0416 0.1144 5752 +5754 2 668.8533 1357.2187 69.0416 0.1144 5753 +5755 2 668.6588 1356.0976 69.0416 0.1144 5754 +5756 2 668.5776 1354.9536 69.0416 0.1144 5755 +5757 2 668.4964 1353.821 69.0416 0.1144 5756 +5758 2 668.4152 1352.677 69.0416 0.1144 5757 +5759 2 668.334 1351.533 69.0416 0.1144 5758 +5760 2 668.2539 1350.4005 69.0416 0.1144 5759 +5761 2 668.1726 1349.2565 69.0416 0.1144 5760 +5762 2 668.1761 1348.1125 69.0416 0.1144 5761 +5763 2 668.3054 1346.9799 69.0416 0.1144 5762 +5764 2 668.4358 1345.8359 69.0416 0.1144 5763 +5765 2 668.5662 1344.7034 69.0416 0.1144 5764 +5766 2 668.6966 1343.5708 69.0416 0.1144 5765 +5767 2 668.827 1342.4268 69.0416 0.1144 5766 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1317.6821 69.9224 0.1144 5788 +5790 2 673.2852 1316.5838 70.2038 0.1144 5789 +5791 2 673.5758 1315.4856 70.4855 0.1144 5790 +5792 2 673.8675 1314.3874 70.7672 0.1144 5791 +5793 2 674.1581 1313.2777 71.0486 0.1144 5792 +5794 2 674.4486 1312.1794 71.3303 0.1144 5793 +5795 2 674.7392 1311.0812 71.6117 0.1144 5794 +5796 2 675.0309 1309.983 71.8934 0.1144 5795 +5797 2 675.3215 1308.8847 72.1748 0.1144 5796 +5798 2 675.6029 1307.775 72.4634 0.1144 5797 +5799 2 675.7482 1306.6539 72.8465 0.1144 5798 +5800 2 675.8935 1305.5328 73.2298 0.1144 5799 +5801 2 676.0388 1304.4117 73.6128 0.1144 5800 +5802 2 676.1841 1303.2791 73.9962 0.1144 5801 +5803 2 676.3294 1302.158 74.3792 0.1144 5802 +5804 2 676.4747 1301.0369 74.7625 0.1144 5803 +5805 2 676.6199 1299.9158 75.1456 0.1144 5804 +5806 2 676.7652 1298.7832 75.5289 0.1144 5805 +5807 2 676.9105 1297.6621 75.9119 0.1144 5806 +5808 2 677.057 1296.541 76.2952 0.1144 5807 +5809 2 677.2022 1295.4198 76.6783 0.1144 5808 +5810 2 677.3475 1294.2873 77.0616 0.1144 5809 +5811 2 677.4928 1293.1662 77.4446 0.1144 5810 +5812 2 677.6381 1292.045 77.8277 0.1144 5811 +5813 2 677.852 1290.9354 78.1894 0.1144 5812 +5814 2 678.2261 1289.86 78.5011 0.1144 5813 +5815 2 678.6014 1288.7846 78.8124 0.1144 5814 +5816 2 678.9754 1287.7093 79.1238 0.1144 5815 +5817 2 679.3507 1286.6454 79.4352 0.1144 5816 +5818 2 679.7248 1285.57 79.7468 0.1144 5817 +5819 2 680.1 1284.4946 80.0582 0.1144 5818 +5820 2 680.4741 1283.4193 80.3695 0.1144 5819 +5821 2 680.8493 1282.3439 80.6809 0.1144 5820 +5822 2 681.2234 1281.28 80.9925 0.1144 5821 +5823 2 681.5986 1280.2046 81.3039 0.1144 5822 +5824 2 681.9727 1279.1293 81.6152 0.1144 5823 +5825 2 682.348 1278.0539 81.9266 0.1144 5824 +5826 2 682.722 1276.9786 82.2382 0.1144 5825 +5827 2 683.0973 1275.9032 82.5496 0.1144 5826 +5828 2 682.7735 1274.9079 82.6238 0.1144 5827 +5829 2 682.4292 1273.8097 82.6238 0.1144 5828 +5830 2 682.158 1272.7572 83.4672 0.1144 5829 +5831 2 681.8892 1271.7047 84.3427 0.1144 5830 +5832 2 681.6215 1270.6522 85.218 0.1144 5831 +5833 2 681.3664 1269.5998 85.9692 0.1144 5832 +5834 2 681.2577 1268.4786 85.384 0.1144 5833 +5835 2 681.149 1267.369 84.7988 0.1144 5834 +5836 2 681.0404 1266.2593 84.2134 0.1144 5835 +5837 2 680.9328 1265.1382 83.6282 0.1144 5836 +5838 2 680.8241 1264.0285 83.0427 0.1144 5837 +5839 2 680.7155 1262.9188 82.4575 0.1144 5838 +5840 2 680.6068 1261.7977 81.872 0.1144 5839 +5841 2 680.4981 1260.688 81.2868 0.1144 5840 +5842 2 680.3894 1259.5783 80.7013 0.1144 5841 +5843 2 680.3071 1258.503 80.8965 0.1144 5842 +5844 2 680.259 1257.4848 82.1836 0.1144 5843 +5845 2 680.211 1256.4781 83.4708 0.1144 5844 +5846 2 680.1629 1255.4599 84.758 0.1144 5845 +5847 2 680.1149 1254.4418 86.0451 0.1144 5846 +5848 2 680.0668 1253.4236 87.3323 0.1144 5847 +5849 2 680.0188 1252.4169 88.6197 0.1144 5848 +5850 2 679.9707 1251.3987 89.9069 0.1144 5849 +5851 2 679.9227 1250.3806 91.194 0.1144 5850 +5852 2 679.8746 1249.3738 92.4812 0.1144 5851 +5853 2 679.8266 1248.3557 93.7684 0.1144 5852 +5854 2 679.8334 1247.7722 96.0996 0.1144 5853 +5855 2 679.8495 1247.246 98.5936 0.1144 5854 +5856 2 679.8655 1246.7312 101.0873 0.1144 5855 +5857 2 680.1286 1246.285 103.2114 0.1144 5856 +5858 2 681.1948 1246.1134 104.1278 0.1144 5857 +5859 2 682.261 1245.9418 105.0445 0.1144 5858 +5860 2 683.3284 1245.7588 105.961 0.1144 5859 +5861 2 684.3946 1245.5872 106.8774 0.1144 5860 +5862 2 685.4619 1245.4156 107.7938 0.1144 5861 +5863 2 686.5281 1245.244 108.7103 0.1144 5862 +5864 2 687.5955 1245.061 109.6267 0.1144 5863 +5865 2 688.6617 1244.8894 110.5432 0.1144 5864 +5866 2 689.7279 1244.7178 111.4596 0.1144 5865 +5867 2 690.7952 1244.5347 112.376 0.1144 5866 +5868 2 691.8615 1244.3631 113.2928 0.1144 5867 +5869 2 692.9288 1244.1915 114.2092 0.1144 5868 +5870 2 693.995 1244.0085 115.1256 0.1144 5869 +5871 2 694.9628 1243.5623 115.9768 0.1144 5870 +5872 2 695.878 1242.956 116.793 0.1144 5871 +5873 2 696.7921 1242.3611 117.609 0.1144 5872 +5874 2 697.7073 1241.7548 118.4252 0.1144 5873 +5875 2 698.6214 1241.1599 119.2414 0.1144 5874 +5876 2 699.5366 1240.5536 120.0573 0.1144 5875 +5877 2 700.4506 1239.9587 120.8735 0.1144 5876 +5878 2 701.3658 1239.3524 121.6894 0.1144 5877 +5879 2 702.2124 1238.666 122.3858 0.1144 5878 +5880 2 702.9205 1237.7851 122.8368 0.1144 5879 +5881 2 703.6286 1236.9042 123.2876 0.1144 5880 +5882 2 704.6331 1236.4924 123.8731 0.1144 5881 +5883 2 705.705 1236.1721 124.488 0.1144 5882 +5884 2 706.7758 1235.8632 125.1032 0.1144 5883 +5885 2 707.8466 1235.5429 125.7183 0.1144 5884 +5886 2 708.9185 1235.234 126.3335 0.1144 5885 +5887 2 709.9893 1234.9251 126.9486 0.1144 5886 +5888 2 711.0601 1234.6048 127.5635 0.1144 5887 +5889 2 712.132 1234.2959 128.1787 0.1144 5888 +5890 2 713.2028 1233.9756 128.7938 0.1144 5889 +5891 2 714.2736 1233.6667 129.409 0.1144 5890 +5892 2 715.3455 1233.3578 130.0242 0.1144 5891 +5893 2 716.4163 1233.0375 130.639 0.1144 5892 +5894 2 717.4871 1232.7286 131.2542 0.1144 5893 +5895 2 718.559 1232.4198 131.8694 0.1144 5894 +5896 2 719.6298 1232.0994 132.4845 0.1144 5895 +5897 2 720.7006 1231.7906 133.0997 0.1144 5896 +5898 2 721.7725 1231.4702 133.7146 0.1144 5897 +5899 2 722.8433 1231.1614 134.3297 0.1144 5898 +5900 2 723.8763 1230.8296 135.1801 0.1144 5899 +5901 2 724.8556 1230.4978 136.358 0.1144 5900 +5902 2 725.836 1230.1546 137.5363 0.1144 5901 +5903 2 726.8152 1229.8114 138.7142 0.1144 5902 +5904 2 727.7945 1229.4682 139.8922 0.1144 5903 +5905 2 728.7749 1229.125 141.0704 0.1144 5904 +5906 2 729.7542 1228.7818 142.2484 0.1144 5905 +5907 2 730.7334 1228.4386 143.4264 0.1144 5906 +5908 2 731.7138 1228.0954 144.6046 0.1144 5907 +5909 2 732.6931 1227.7522 145.7826 0.1144 5908 +5910 2 733.6724 1227.409 146.9605 0.1144 5909 +5911 2 734.6528 1227.0658 148.1388 0.1144 5910 +5912 2 735.632 1226.7226 149.3167 0.1144 5911 +5913 2 736.6113 1226.3794 150.4947 0.1144 5912 +5914 2 737.5917 1226.0362 151.6729 0.1144 5913 +5915 2 738.571 1225.693 152.8509 0.1144 5914 +5916 2 739.5594 1225.3613 153.8869 0.1144 5915 +5917 2 740.6428 1225.0524 153.3885 0.1144 5916 +5918 2 741.7261 1224.7435 152.8901 0.1144 5917 +5919 2 742.8095 1224.4346 152.3917 0.1144 5918 +5920 2 743.8929 1224.1372 151.8936 0.1144 5919 +5921 2 744.9762 1223.8283 151.3952 0.1144 5920 +5922 2 746.0596 1223.5194 150.8968 0.1144 5921 +5923 2 747.143 1223.2106 150.3984 0.1144 5922 +5924 2 748.2263 1222.9131 149.9 0.1144 5923 +5925 2 749.3097 1222.6042 149.4016 0.1144 5924 +5926 2 688.3951 1478.5857 59.0296 0.1144 5638 +5927 2 688.4603 1477.476 59.6868 0.1144 5926 +5928 2 688.5267 1476.3663 60.3439 0.1144 5927 +5929 2 688.5919 1475.2566 61.0011 0.1144 5928 +5930 2 688.6571 1474.147 61.6582 0.1144 5929 +5931 2 688.7223 1473.0373 62.3154 0.1144 5930 +5932 2 688.7875 1471.9276 62.9726 0.1144 5931 +5933 2 688.8539 1470.8179 63.6297 0.1144 5932 +5934 2 688.9191 1469.7082 64.2869 0.1144 5933 +5935 2 688.9843 1468.5986 64.944 0.1144 5934 +5936 2 689.0495 1467.4889 65.6012 0.1144 5935 +5937 2 689.1147 1466.3792 66.2584 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695.2397 1442.7899 71.3348 0.1144 5958 +5960 2 695.1013 1441.6574 71.5646 0.1144 5959 +5961 2 694.9628 1440.5248 71.7942 0.1144 5960 +5962 2 694.8244 1439.3922 72.0238 0.1144 5961 +5963 2 694.686 1438.2597 72.2534 0.1144 5962 +5964 2 694.5476 1437.1271 72.4833 0.1144 5963 +5965 2 694.4091 1435.9946 72.7129 0.1144 5964 +5966 2 694.2707 1434.862 72.9425 0.1144 5965 +5967 2 694.1323 1433.7294 73.1721 0.1144 5966 +5968 2 693.9939 1432.6083 73.402 0.1144 5967 +5969 2 693.8554 1431.4758 73.6316 0.1144 5968 +5970 2 693.717 1430.3432 73.8612 0.1144 5969 +5971 2 693.5775 1429.2106 74.0908 0.1144 5970 +5972 2 693.439 1428.0781 74.3207 0.1144 5971 +5973 2 693.3006 1426.9455 74.5503 0.1144 5972 +5974 2 693.2011 1425.813 74.6175 0.1144 5973 +5975 2 693.1736 1424.669 74.375 0.1144 5974 +5976 2 693.1473 1423.5364 74.1325 0.1144 5975 +5977 2 693.1199 1422.3924 73.8903 0.1144 5976 +5978 2 693.0935 1421.2484 73.6478 0.1144 5977 +5979 2 693.0672 1420.1158 73.4054 0.1144 5978 +5980 2 693.0398 1418.9718 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0.1144 6493 +6495 2 715.691 1315.4856 69.5559 0.1144 6494 +6496 2 715.7836 1314.353 69.991 0.1144 6495 +6497 2 715.8763 1313.2319 70.4259 0.1144 6496 +6498 2 715.969 1312.0994 70.8607 0.1144 6497 +6499 2 716.0616 1310.9782 71.2956 0.1144 6498 +6500 2 716.1543 1309.8457 71.7304 0.1144 6499 +6501 2 716.247 1308.7246 72.1652 0.1144 6500 +6502 2 716.3396 1307.592 72.6001 0.1144 6501 +6503 2 716.4323 1306.4709 73.0349 0.1144 6502 +6504 2 716.525 1305.3383 73.4698 0.1144 6503 +6505 2 716.6176 1304.2172 73.9049 0.1144 6504 +6506 2 716.7091 1303.0961 74.3397 0.1144 6505 +6507 2 716.8018 1301.9635 74.7746 0.1144 6506 +6508 2 716.8945 1300.8424 75.2094 0.1144 6507 +6509 2 716.9871 1299.7098 75.6442 0.1144 6508 +6510 2 717.0798 1298.5887 76.0791 0.1144 6509 +6511 2 717.1725 1297.4562 76.5139 0.1144 6510 +6512 2 717.2651 1296.335 76.9488 0.1144 6511 +6513 2 717.3578 1295.2025 77.3836 0.1144 6512 +6514 2 717.4504 1294.0814 77.8187 0.1144 6513 +6515 2 717.5431 1292.9488 78.2536 0.1144 6514 +6516 2 717.6358 1291.8277 78.6884 0.1144 6515 +6517 2 717.7284 1290.7066 79.1232 0.1144 6516 +6518 2 717.8211 1289.574 79.5581 0.1144 6517 +6519 2 717.9138 1288.4529 79.9929 0.1144 6518 +6520 2 718.0064 1287.3203 80.4278 0.1144 6519 +6521 2 718.0991 1286.1992 80.8626 0.1144 6520 +6522 2 718.1918 1285.0666 81.2974 0.1144 6521 +6523 2 718.2844 1283.9455 81.7326 0.1144 6522 +6524 2 718.3771 1282.813 82.1674 0.1144 6523 +6525 2 718.4698 1281.6918 82.6022 0.1144 6524 +6526 2 718.4915 1280.5478 82.8887 0.1144 6525 +6527 2 718.5098 1279.4153 83.1673 0.1144 6526 +6528 2 718.5281 1278.2713 83.4462 0.1144 6527 +6529 2 718.5464 1277.1387 83.7248 0.1144 6528 +6530 2 718.5636 1275.9947 84.0036 0.1144 6529 +6531 2 718.5819 1274.8622 84.2822 0.1144 6530 +6532 2 718.6002 1273.7296 84.5608 0.1144 6531 +6533 2 718.6185 1272.5856 84.8397 0.1144 6532 +6534 2 718.6368 1271.453 85.1183 0.1144 6533 +6535 2 718.6551 1270.309 85.3972 0.1144 6534 +6536 2 718.6722 1269.1765 85.6758 0.1144 6535 +6537 2 718.6905 1268.0325 85.9544 0.1144 6536 +6538 2 718.5144 1266.9114 85.8816 0.1144 6537 +6539 2 718.2798 1265.7902 85.7027 0.1144 6538 +6540 2 718.0442 1264.6806 85.5235 0.1144 6539 +6541 2 717.8097 1263.5594 85.3446 0.1144 6540 +6542 2 717.574 1262.4383 85.1656 0.1144 6541 +6543 2 717.3395 1261.3286 84.9867 0.1144 6542 +6544 2 717.105 1260.2075 84.8075 0.1144 6543 +6545 2 716.8693 1259.0864 84.6286 0.1144 6544 +6546 2 716.6348 1257.9767 84.4497 0.1144 6545 +6547 2 716.3991 1256.8556 84.2708 0.1144 6546 +6548 2 716.1646 1255.7345 84.0916 0.1144 6547 +6549 2 715.9289 1254.6248 83.9126 0.1144 6548 +6550 2 715.7127 1253.5037 83.8054 0.1144 6549 +6551 2 715.6258 1252.3711 84.2122 0.1144 6550 +6552 2 715.5388 1251.25 84.6194 0.1144 6551 +6553 2 715.453 1250.1174 85.0265 0.1144 6552 +6554 2 715.3661 1248.9849 85.4333 0.1144 6553 +6555 2 715.2791 1247.8638 85.8404 0.1144 6554 +6556 2 715.1933 1246.7312 86.2476 0.1144 6555 +6557 2 715.1064 1245.5986 86.6544 0.1144 6556 +6558 2 715.0194 1244.4775 87.0615 0.1144 6557 +6559 2 714.9336 1243.345 87.4686 0.1144 6558 +6560 2 714.8467 1242.2124 87.8758 0.1144 6559 +6561 2 714.7598 1241.0913 88.2826 0.1144 6560 +6562 2 714.6728 1239.9587 88.6897 0.1144 6561 +6563 2 714.587 1238.8376 89.0968 0.1144 6562 +6564 2 714.5001 1237.705 89.5037 0.1144 6563 +6565 2 714.4131 1236.5725 89.9108 0.1144 6564 +6566 2 714.3273 1235.4514 90.3179 0.1144 6565 +6567 2 714.2404 1234.3188 90.7248 0.1144 6566 +6568 2 714.1534 1233.1862 91.1319 0.1144 6567 +6569 2 714.0676 1232.0651 91.539 0.1144 6568 +6570 2 713.9807 1230.9326 91.9461 0.1144 6569 +6571 2 713.8938 1229.8 92.353 0.1144 6570 +6572 2 713.808 1228.6789 92.7601 0.1144 6571 +6573 2 713.721 1227.5463 93.1672 0.1144 6572 +6574 2 713.6341 1226.4138 93.574 0.1144 6573 +6575 2 713.5483 1225.2926 93.9812 0.1144 6574 +6576 2 713.4613 1224.1601 94.3883 0.1144 6575 +6577 2 713.3744 1223.0275 94.7954 0.1144 6576 +6578 2 713.2874 1221.9064 95.2022 0.1144 6577 +6579 2 713.2016 1220.7738 95.6094 0.1144 6578 +6580 2 713.1147 1219.6527 96.0165 0.1144 6579 +6581 2 713.0277 1218.5202 96.4233 0.1144 6580 +6582 2 712.9419 1217.3876 96.8304 0.1144 6581 +6583 2 712.855 1216.2665 97.2376 0.1144 6582 +6584 2 712.7681 1215.1339 97.6444 0.1144 6583 +6585 2 712.6823 1214.0014 98.0515 0.1144 6584 +6586 2 712.5953 1212.8802 98.4586 0.1144 6585 +6587 2 712.5084 1211.7477 98.8658 0.1144 6586 +6588 2 712.4226 1210.6151 99.2726 0.1144 6587 +6589 2 712.3356 1209.494 99.6797 0.1144 6588 +6590 2 712.2487 1208.3614 100.0868 0.1144 6589 +6591 2 712.1617 1207.2289 100.4937 0.1144 6590 +6592 2 712.0759 1206.1078 100.9008 0.1144 6591 +6593 2 711.989 1204.9752 101.3079 0.1144 6592 +6594 2 711.902 1203.8426 101.7148 0.1144 6593 +6595 2 711.8162 1202.7215 102.1219 0.1144 6594 +6596 2 711.7293 1201.589 102.529 0.1144 6595 +6597 2 711.6424 1200.4678 102.9361 0.1144 6596 +6598 2 711.5566 1199.3353 103.343 0.1144 6597 +6599 2 711.4696 1198.2027 103.7501 0.1144 6598 +6600 2 711.3827 1197.0816 104.1572 0.1144 6599 +6601 2 711.2969 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665.4351 1312.2138 71.0884 0.3383 11527 +11529 4 665.2463 1312.0193 69.939 0.3381 11528 +11530 4 665.3241 1310.8867 69.4708 0.3378 11529 +11531 4 665.4019 1309.7656 69.0024 0.3376 11530 +11532 4 665.3104 1308.7131 68.2993 0.3374 11531 +11533 4 665.0186 1307.6492 67.9594 0.3371 11532 +11534 4 664.6205 1306.5853 67.9098 0.3369 11533 +11535 4 663.8701 1305.7616 67.9098 0.3366 11534 +11536 4 663.7179 1304.6405 67.9098 0.3363 11535 +11537 4 663.8209 1303.5079 67.9098 0.3361 11536 +11538 4 664.4592 1302.5698 67.9098 0.3359 11537 +11539 4 664.5565 1301.4487 67.9098 0.3356 11538 +11540 4 664.5748 1300.3047 67.9098 0.3354 11539 +11541 4 664.5931 1299.1607 67.9098 0.3352 11540 +11542 4 664.6823 1298.0739 68.6507 0.335 11541 +11543 4 665.2188 1297.1015 69.0329 0.3347 11542 +11544 4 666.1912 1296.5181 69.0416 0.3344 11543 +11545 4 667.1934 1296.049 69.0416 0.3342 11544 +11546 4 667.651 1295.008 69.0416 0.3339 11545 +11547 4 668.5456 1294.3216 69.0416 0.3337 11546 +11548 4 669.3544 1293.5094 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0.3775 11588 +11590 4 664.2842 1440.9595 33.7176 0.3775 11589 +11591 4 663.806 1439.9414 33.6958 0.3775 11590 +11592 4 663.6115 1438.866 33.1674 0.3775 11591 +11593 4 663.5989 1437.7563 32.6085 0.3775 11592 +11594 4 663.568 1436.6466 32.0841 0.3775 11593 +11595 4 663.3713 1435.5484 31.4426 0.3775 11594 +11596 4 663.0624 1434.4616 31.08 0.3775 11595 +11597 4 662.8645 1433.3405 31.0752 0.3775 11596 +11598 4 662.7638 1432.2079 30.9019 0.3775 11597 +11599 4 662.6368 1431.0868 30.52 0.3775 11598 +11600 4 662.5579 1429.9542 30.6174 0.3775 11599 +11601 4 662.2902 1428.856 30.8 0.3775 11600 +11602 4 661.8704 1427.7921 30.8 0.3775 11601 +11603 4 661.5374 1426.7053 30.8 0.3775 11602 +11604 4 661.0375 1425.6871 30.8 0.3775 11603 +11605 4 660.4278 1424.7262 30.8 0.3775 11604 +11606 4 659.9278 1423.6966 30.8 0.3775 11605 +11607 4 659.254 1422.8042 30.7894 0.3775 11606 +11608 4 658.2645 1422.4725 30.6424 0.3775 11607 +11609 4 657.3332 1422.0492 30.52 0.3775 11608 +11610 4 656.5908 1421.1798 30.52 0.3775 11609 +11611 4 656.2064 1420.1273 30.52 0.3775 11610 +11612 4 655.7991 1419.0519 30.52 0.3775 11611 +11613 4 655.2249 1418.0681 30.5194 0.3775 11612 +11614 4 654.4687 1417.2215 30.2974 0.3775 11613 +11615 4 653.693 1416.3978 30.0157 0.3775 11614 +11616 4 652.9483 1415.5284 29.9603 0.3775 11615 +11617 4 652.0686 1414.8077 30.1935 0.3775 11616 +11618 4 651.3101 1413.9726 30.5242 0.3775 11617 +11619 4 650.7907 1412.9773 31.0159 0.3775 11618 +11620 4 650.2793 1411.9591 31.08 0.3775 11619 +11621 4 649.4133 1411.227 31.08 0.3775 11620 +11622 4 648.5462 1410.4834 31.08 0.3775 11621 +11623 4 647.766 1409.6482 31.0808 0.3775 11622 +11624 4 647.115 1408.7674 31.8632 0.3775 11623 +11625 4 646.2467 1408.0238 31.92 0.3775 11624 +11626 4 645.4459 1407.2115 31.92 0.3775 11625 +11627 4 644.8739 1406.2162 31.9194 0.3775 11626 +11628 4 644.3168 1405.221 31.8578 0.3775 11627 +11629 4 643.6476 1404.363 31.0968 0.3775 11628 +11630 4 643.166 1403.3448 31.08 0.3775 11629 +11631 4 642.213 1402.7156 31.0797 0.3775 11630 +11632 4 641.315 1402.0292 30.9565 0.3775 11631 +11633 4 640.4501 1401.4458 30.24 0.3775 11632 +11634 4 640.0497 1400.3818 30.2434 0.3775 11633 +11635 4 639.6745 1399.3294 30.4688 0.3775 11634 +11636 4 638.9801 1398.5057 31.3866 0.3775 11635 +11637 4 638.2056 1397.7278 31.9197 0.3775 11636 +11638 4 637.5718 1396.7782 31.726 0.3775 11637 +11639 4 637.4814 1395.68 31.5216 0.3775 11638 +11640 4 637.3167 1394.5589 31.36 0.3775 11639 +11641 4 636.8362 1393.5407 31.36 0.3775 11640 +11642 4 636.1956 1392.5912 31.36 0.3775 11641 +11643 4 635.6945 1391.573 31.36 0.3775 11642 +11644 4 635.4154 1390.4634 31.36 0.3775 11643 +11645 4 635.0321 1389.4223 31.36 0.3775 11644 +11646 4 634.4613 1388.427 31.3642 0.3775 11645 +11647 4 633.9236 1387.4203 31.5034 0.3775 11646 +11648 4 633.5197 1386.3678 31.638 0.3775 11647 +11649 4 633.2349 1385.2696 31.4902 0.3775 11648 +11650 4 632.8757 1384.2057 31.045 0.3775 11649 +11651 4 632.2453 1383.2905 30.8 0.3775 11650 +11652 4 631.472 1382.4439 30.8 0.3775 11651 +11653 4 630.9904 1381.4372 30.8 0.3775 11652 +11654 4 630.7536 1380.3161 30.8 0.3775 11653 +11655 4 630.2571 1379.3208 30.8003 0.3775 11654 +11656 4 629.7663 1378.2912 30.8 0.3775 11655 +11657 4 629.6553 1377.1701 30.8764 0.3775 11656 +11658 4 629.2618 1376.1405 31.3664 0.3775 11657 +11659 4 628.5548 1375.2596 31.6154 0.3775 11658 +11660 4 627.595 1374.6647 31.64 0.3775 11659 +11661 4 626.8914 1373.7724 31.64 0.3775 11660 +11662 4 626.1512 1372.903 31.64 0.3775 11661 +11663 4 625.2692 1372.1708 31.64 0.3775 11662 +11664 4 624.5004 1371.3357 31.64 0.3775 11663 +11665 4 624.2328 1370.2374 31.3012 0.3775 11664 +11666 4 623.5246 1369.3566 30.802 0.3775 11665 +11667 4 622.6609 1368.613 30.8 0.3775 11666 +11668 4 621.8624 1367.8007 30.8 0.3775 11667 +11669 4 620.8465 1367.2859 30.8 0.3775 11668 +11670 4 620.1933 1366.3478 30.8 0.3775 11669 +11671 4 619.738 1365.2954 30.8 0.3775 11670 +11672 4 619.2678 1364.2543 30.7728 0.3775 11671 +11673 4 618.7907 1363.2362 30.2414 0.3775 11672 +11674 4 618.2107 1362.2523 30.24 0.3775 11673 +11675 4 617.4946 1361.36 30.24 0.3775 11674 +11676 4 616.751 1360.4906 30.24 0.3775 11675 +11677 4 616.1561 1359.5182 30.24 0.3775 11676 +11678 4 615.6299 1358.5458 30.1546 0.3775 11677 +11679 4 614.6426 1358.0081 29.671 0.3775 11678 +11680 4 613.581 1357.5962 29.573 0.3775 11679 +11681 4 612.6669 1356.9327 29.3266 0.3775 11680 +11682 4 611.9439 1356.0633 29.0212 0.3775 11681 +11683 4 611.2838 1355.1366 28.7123 0.3775 11682 +11684 4 610.4796 1354.3587 28.5603 0.3775 11683 +11685 4 609.5472 1353.7066 28.56 0.3775 11684 +11686 4 608.9215 1352.8029 28.3374 0.3775 11685 +11687 4 608.6011 1351.7275 28.0022 0.3775 11686 +11688 4 608.2968 1350.6178 28.0 0.3775 11687 +11689 4 608.1962 1349.4967 28.0 0.3775 11688 +11690 4 608.2923 1348.3527 28.0003 0.3775 11689 +11691 4 608.3026 1347.2202 28.0 0.3775 11690 +11692 4 608.1698 1346.0762 28.0 0.3775 11691 +11693 4 608.1241 1344.9436 28.0022 0.3775 11692 +11694 4 608.1824 1343.7996 28.1588 0.3775 11693 +11695 4 608.1984 1342.667 28.28 0.3775 11694 +11696 4 607.8404 1341.626 28.0963 0.3775 11695 +11697 4 607.1025 1340.8366 27.3507 0.3775 11696 +11698 4 606.5923 1339.8871 26.9206 0.3775 11697 +11699 4 606.1004 1338.8575 26.9427 0.3775 11698 +11700 4 605.5547 1337.9423 27.8354 0.3775 11699 +11701 4 604.9804 1336.9928 28.4934 0.3775 11700 +11702 4 604.4278 1335.9975 28.7778 0.3775 11701 +11703 4 603.7712 1335.0594 28.838 0.3775 11702 +11704 4 603.0379 1334.3387 29.9421 0.3775 11703 +11705 4 602.0334 1333.801 29.96 0.3775 11704 +11706 4 601.0542 1333.4922 30.2022 0.3775 11705 +11707 4 601.0565 1332.7371 32.2109 0.3775 11706 +11708 4 600.4398 1331.902 33.1926 0.3775 11707 +11709 4 599.5487 1331.2042 33.53 0.3775 11708 +11710 4 598.7284 1330.4148 33.6042 0.3775 11709 +11711 4 598.0512 1329.511 33.8568 0.3775 11710 +11712 4 597.43 1328.5958 34.5881 0.3775 11711 +11713 4 596.9975 1327.6006 35.1655 0.3775 11712 +11714 4 596.9918 1326.4794 35.4679 0.3775 11713 +11715 4 597.3888 1325.427 35.56 0.3775 11714 +11716 4 598.1496 1324.5918 35.56 0.3775 11715 +11717 4 599.1711 1324.1228 35.56 0.3775 11716 +11718 4 600.1985 1323.6309 35.56 0.3775 11717 +11719 4 601.2864 1323.3106 35.5667 0.3775 11718 +11720 4 602.4201 1323.2648 35.7913 0.3775 11719 +11721 4 603.444 1322.8186 36.09 0.3775 11720 +11722 4 604.2562 1322.0178 36.12 0.3775 11721 +11723 4 604.1041 1320.931 36.12 0.3775 11722 +11724 4 603.3891 1320.0387 36.12 0.3775 11723 +11725 4 602.8411 1319.0434 36.12 0.3775 11724 +11726 4 602.4716 1317.9566 36.12 0.3775 11725 +11727 4 602.4933 1316.8584 36.6971 0.3775 11726 +11728 4 602.9418 1315.9203 37.8048 0.3775 11727 +11729 4 603.3948 1315.0509 39.2126 0.3775 11728 +11730 4 603.984 1314.1471 39.9532 0.3775 11729 +11731 4 604.7996 1313.3578 40.2416 0.3775 11730 +11732 4 605.7938 1312.843 40.3239 0.3775 11731 +11733 4 606.8119 1312.4197 40.815 0.3775 11732 +11734 4 607.4388 1311.5617 41.16 0.3775 11733 +11735 4 607.8164 1310.4749 41.16 0.3775 11734 +11736 4 608.2065 1309.3995 41.16 0.3775 11735 +11737 4 608.6755 1308.3585 41.16 0.3775 11736 +11738 4 609.2006 1307.3403 41.16 0.3775 11737 +11739 4 609.7577 1306.3565 41.44 0.3775 11738 +11740 4 610.3103 1305.3612 41.3977 0.3775 11739 +11741 4 610.3469 1304.263 40.6378 0.3775 11740 +11742 4 610.3469 1303.1304 40.32 0.3775 11741 +11743 4 610.4739 1301.9978 40.32 0.3775 11742 +11744 4 610.7679 1300.911 40.32 0.3775 11743 +11745 4 611.3879 1299.973 40.0593 0.3775 11744 +11746 4 611.9428 1299.0578 39.4831 0.3775 11745 +11747 4 612.0251 1297.9366 39.48 0.3775 11746 +11748 4 612.064 1296.8155 39.48 0.3775 11747 +11749 4 612.3924 1295.7287 39.2596 0.3775 11748 +11750 4 612.6429 1294.6305 38.8189 0.3775 11749 +11751 4 612.6681 1293.4979 38.6434 0.3775 11750 +11752 4 612.5994 1292.3654 38.64 0.3775 11751 +11753 4 612.5754 1291.2214 38.64 0.3775 11752 +11754 4 612.6646 1290.0774 38.64 0.3775 11753 +11755 4 613.0261 1289.0134 38.6288 0.3775 11754 +11756 4 613.7263 1288.144 38.4863 0.3775 11755 +11757 4 614.5122 1287.3203 38.2049 0.3775 11756 +11758 4 615.1048 1286.531 37.0244 0.3775 11757 +11759 4 616.0291 1286.0962 36.4157 0.3775 11758 +11760 4 616.9272 1286.6911 36.4 0.3775 11759 +11761 4 617.4763 1287.6864 36.4 0.3775 11760 +11762 4 618.5093 1287.7894 37.0922 0.3775 11761 +11763 4 619.3616 1287.3546 36.3756 0.3775 11762 +11764 4 619.9393 1286.6682 35.0868 0.3775 11763 +11765 4 619.3719 1285.673 35.0868 0.3775 11764 +11766 4 619.5584 1284.7806 34.0035 0.3775 11765 +11767 4 620.0537 1283.8998 32.823 0.3775 11766 +11768 4 620.6921 1282.9502 32.823 0.3775 11767 +11769 4 620.8477 1281.9664 32.823 0.3775 11768 +11770 4 620.3889 1280.9139 32.823 0.3775 11769 +11771 4 619.9302 1279.8729 32.823 0.3775 11770 +11772 4 620.0057 1278.8547 32.6119 0.3775 11771 +11773 4 620.5651 1277.8709 32.2095 0.3775 11772 +11774 4 621.1245 1276.887 31.8072 0.3775 11773 +11775 4 621.7274 1275.9375 31.3253 0.3775 11774 +11776 4 622.3474 1274.9994 30.8112 0.3775 11775 +11777 4 622.9675 1274.0614 30.2971 0.3775 11776 +11778 4 623.5875 1273.1233 29.783 0.3775 11777 +11779 4 624.2282 1272.1852 29.4277 0.3775 11778 +11780 4 624.9123 1271.27 29.4277 0.3775 11779 +11781 4 625.5953 1270.3548 29.4277 0.3775 11780 +11782 4 626.2794 1269.4396 29.4277 0.3775 11781 +11783 4 626.9635 1268.5244 29.4277 0.3775 11782 +11784 4 627.6476 1267.5978 29.4277 0.3775 11783 +11785 4 628.3317 1266.6826 29.4277 0.3775 11784 +11786 4 628.9403 1265.733 29.1844 0.3775 11785 +11787 4 629.4883 1264.7378 28.74 0.3775 11786 +11788 4 630.0363 1263.7539 28.2957 0.3775 11787 +11789 4 665.8023 1476.5265 36.512 0.9438 8791 +11790 4 665.0072 1476.1947 38.3622 0.9276 11789 +11791 4 664.4615 1475.6799 40.1363 0.9113 11790 +11792 4 664.4375 1474.7418 41.7508 0.8951 11791 +11793 4 664.4123 1473.8038 43.3656 0.8788 11792 +11794 4 663.8335 1473.1746 44.1414 0.8626 11793 +11795 4 662.7421 1472.8428 44.1414 0.8463 11794 +11796 4 662.495 1472.0534 44.6169 0.8301 11795 +11797 4 662.7775 1470.9895 45.39 0.814 11796 +11798 4 663.059 1469.9256 46.1633 0.7977 11797 +11799 4 663.8724 1469.4108 46.9938 0.7815 11798 +11800 4 664.8116 1468.9303 47.7114 0.7652 11799 +11801 4 665.5495 1468.0723 48.1877 0.749 11800 +11802 4 666.2873 1467.2258 48.6643 0.7327 11801 +11803 4 665.6124 1466.3563 49.3483 0.7165 11802 +11804 4 664.9248 1465.4869 50.034 0.7002 11803 +11805 4 664.2373 1464.6174 50.72 0.684 11804 +11806 4 664.3849 1463.645 50.9323 0.6678 11805 +11807 4 664.9077 1462.6269 50.9323 0.6515 11806 +11808 4 665.9087 1462.1807 50.9323 0.6353 11807 +11809 4 667.0035 1461.849 50.9323 0.619 11808 +11810 4 668.0983 1461.5172 50.9323 0.6028 11809 +11811 4 667.8558 1460.9109 51.525 0.5865 11810 +11812 4 667.0389 1460.1902 52.3718 0.5703 11811 +11813 4 666.2622 1459.458 53.235 0.5542 11812 +11814 4 667.1053 1458.886 54.1481 0.5379 11813 +11815 4 667.7379 1458.0966 54.3278 0.5217 11814 +11816 4 668.1006 1457.0327 54.3278 0.5054 11815 +11817 4 669.1267 1456.5408 54.3278 0.4892 11816 +11818 4 669.3338 1455.6142 54.6263 0.4729 11817 +11819 4 669.2091 1454.4816 55.043 0.4567 11818 +11820 4 669.0833 1453.3605 55.4596 0.4404 11819 +11821 4 669.5569 1452.3766 54.999 0.4404 11820 +11822 4 670.0465 1451.3585 54.5227 0.4404 11821 +11823 4 670.7112 1450.5348 54.9604 0.4404 11822 +11824 4 671.4971 1449.8255 56.0316 0.4404 11823 +11825 4 672.283 1449.1162 57.1029 0.4404 11824 +11826 4 673.069 1448.407 58.1742 0.4404 11825 +11827 4 673.4911 1447.5718 59.1875 0.4404 11826 +11828 4 673.276 1446.5194 60.0995 0.4404 11827 +11829 4 673.061 1445.4554 61.0117 0.4404 11828 +11830 4 674.0242 1445.3754 60.6315 0.4404 11829 +11831 4 675.1442 1445.4211 60.0788 0.4404 11830 +11832 4 676.2653 1445.4783 59.526 0.4404 11831 +11833 4 677.3853 1445.5241 58.9736 0.4404 11832 +11834 4 677.8898 1444.8148 59.997 0.4404 11833 +11835 4 678.2261 1443.8996 61.4505 0.4404 11834 +11836 4 678.5636 1442.973 62.9037 0.4404 11835 +11837 4 678.8999 1442.0578 64.3572 0.4404 11836 +11838 4 679.2969 1441.2112 65.8291 0.4404 11837 +11839 4 680.1641 1440.868 67.4478 0.4404 11838 +11840 4 681.0278 1440.5134 69.0416 0.4404 11839 +11841 4 681.6455 1439.5524 69.0416 0.4404 11840 +11842 4 682.2633 1438.58 69.0416 0.4404 11841 +11843 4 682.3754 1437.619 69.5755 0.4404 11842 +11844 4 681.9498 1436.6581 70.6762 0.4404 11843 +11845 4 681.4042 1435.7543 71.6915 0.4404 11844 +11846 4 680.6972 1434.9421 72.5931 0.4404 11845 +11847 4 679.989 1434.1184 73.4944 0.4404 11846 +11848 4 679.0978 1434.0726 75.1318 0.4404 11847 +11849 4 678.1907 1434.107 76.8354 0.4404 11848 +11850 4 678.2124 1433.1002 77.7636 0.4404 11849 +11851 4 678.3096 1432.0249 78.6282 0.4404 11850 +11852 4 678.408 1430.9381 79.4928 0.4404 11851 +11853 4 678.5053 1429.8513 80.3575 0.4404 11852 +11854 4 678.6025 1428.7759 81.2221 0.4404 11853 +11855 4 678.7638 1427.7349 82.3348 0.4404 11854 +11856 4 678.9526 1426.7282 83.5598 0.4404 11855 +11857 4 679.1413 1425.71 84.7848 0.4404 11856 +11858 4 679.8803 1425.0236 86.0059 0.4404 11857 +11859 4 680.6686 1424.3601 87.2267 0.4404 11858 +11860 4 681.3893 1423.6394 88.3459 0.4404 11859 +11861 4 681.681 1422.5526 88.8124 0.4404 11860 +11862 4 681.9727 1421.4658 89.2791 0.4404 11861 +11863 4 682.2644 1420.3675 89.7459 0.4404 11862 +11864 4 682.5562 1419.2807 90.2126 0.4404 11863 +11865 4 682.8479 1418.1939 90.6791 0.4404 11864 +11866 4 683.1396 1417.1071 91.1459 0.4404 11865 +11867 4 683.4313 1416.0089 91.6126 0.4404 11866 +11868 4 682.992 1415.414 93.4662 0.4404 11867 +11869 4 682.4315 1414.8878 95.5475 0.4404 11868 +11870 4 682.007 1414.2929 97.293 0.4404 11869 +11871 4 682.4212 1413.2404 96.9758 0.4404 11870 +11872 4 682.8342 1412.1765 96.6582 0.4404 11871 +11873 4 683.2471 1411.1126 96.3407 0.4404 11872 +11874 4 683.4164 1410.0258 96.5328 0.4404 11873 +11875 4 683.405 1408.9046 97.102 0.4404 11874 +11876 4 683.3936 1407.7835 97.671 0.4404 11875 +11877 4 683.381 1406.6624 98.2402 0.4404 11876 +11878 4 683.3695 1405.5413 98.8095 0.4404 11877 +11879 4 683.3581 1404.4202 99.3787 0.4404 11878 +11880 4 684.017 1403.9969 100.126 0.4404 11879 +11881 4 685.105 1404.0083 100.9876 0.4404 11880 +11882 4 686.1941 1404.0312 101.8492 0.4404 11881 +11883 4 685.4459 1403.2075 101.8648 0.4404 11882 +11884 4 684.7 1402.3838 101.8839 0.4404 11883 +11885 4 685.526 1401.6631 102.725 0.4404 11884 +11886 4 686.3508 1400.9538 103.5658 0.4404 11885 +11887 4 686.9045 1400.0958 104.2258 0.4404 11886 +11888 4 686.9125 1398.9633 104.5198 0.4404 11887 +11889 4 686.9205 1397.8193 104.8135 0.4404 11888 +11890 4 686.9274 1396.6867 105.1075 0.4404 11889 +11891 4 687.2397 1395.6457 105.4777 0.4404 11890 +11892 4 687.8815 1394.719 105.9302 0.4404 11891 +11893 4 688.5233 1393.7924 106.3829 0.4404 11892 +11894 4 688.855 1392.7628 107.3316 0.4404 11893 +11895 4 689.1788 1391.7446 108.2911 0.4404 11894 +11896 4 689.5037 1390.715 109.2504 0.4404 11895 +11897 4 690.11 1389.9142 110.1542 0.4404 11896 +11898 4 691.0732 1389.3994 110.9872 0.4404 11897 +11899 4 692.0376 1388.8846 111.8205 0.4404 11898 +11900 4 691.9301 1388.0152 112.2839 0.4404 11899 +11901 4 691.4119 1387.0085 112.6056 0.4404 11900 +11902 4 690.8948 1385.9903 112.9276 0.4404 11901 +11903 4 690.6877 1385.098 113.3801 0.4404 11902 +11904 4 691.6773 1384.6862 114.336 0.4404 11903 +11905 4 692.6668 1384.2629 115.2917 0.4404 11904 +11906 4 693.6552 1383.8396 116.2473 0.4404 11905 +11907 4 693.9882 1382.9587 116.9582 0.4404 11906 +11908 4 693.9733 1381.8376 117.5395 0.4404 11907 +11909 4 693.9584 1380.7165 118.1205 0.4404 11908 +11910 4 693.9424 1379.6068 118.7018 0.4404 11909 +11911 4 694.4538 1378.6458 119.1873 0.4404 11910 +11912 4 695.1333 1377.7421 119.6423 0.4404 11911 +11913 4 695.6584 1376.7811 119.9741 0.4404 11912 +11914 4 695.7671 1375.6371 119.9741 0.4404 11913 +11915 4 696.0794 1374.5389 119.9741 0.4404 11914 +11916 4 696.1309 1373.4178 119.9741 0.4404 11915 +11917 4 696.2647 1372.3653 120.1654 0.4404 11916 +11918 4 697.0552 1371.6102 120.9869 0.4404 11917 +11919 4 697.8457 1370.8552 121.8084 0.4404 11918 +11920 4 698.6362 1370.0887 122.6302 0.4404 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S1) ; 66 + Normal + | + ( 54.69 261.75 -36.55 0.88 S1) ; 1, R-1-1-1-1-2-1-1-2 + ( 56.74 262.70 -35.47 0.88 S1) ; 2 + ( 57.61 263.34 -32.77 0.88 S1) ; 3 + ( 59.66 263.65 -30.40 0.59 S1) ; 4 + ( 61.12 264.61 -29.88 0.59 S1) ; 5 + ( 62.59 263.97 -28.52 0.59 S1) ; 6 + ( 64.34 264.61 -27.15 0.59 S1) ; 7 + ( 64.93 264.93 -26.15 0.59 S1) ; 8 + ( 65.80 265.88 -26.05 0.59 S1) ; 9 + ( 66.97 267.79 -25.92 0.59 S1) ; 10 + ( 68.73 268.43 -25.07 0.59 S1) ; 11 + ( 69.61 268.11 -23.45 0.59 S1) ; 12 + ( 70.78 268.43 -21.77 0.59 S1) ; 13 + ( 72.24 269.38 -21.67 0.59 S1) ; 14 + ( 73.99 269.38 -21.30 0.59 S1) ; 15 + ( 75.46 269.06 -19.60 0.59 S1) ; 16 + ( 76.04 269.38 -16.67 0.59 S1) ; 17 + ( 77.21 270.34 -15.40 0.59 S1) ; 18 + ( 79.26 271.29 -13.92 0.59 S1) ; 19 + ( 81.31 272.56 -13.23 0.59 S1) ; 20 + ( 82.19 273.84 -12.75 0.59 S1) ; 21 + ( 84.23 275.43 -12.30 0.59 S1) ; 22 + ( 85.99 275.11 -11.68 0.59 S1) ; 23 + ( 85.70 276.06 -10.30 0.59 S1) ; 24 + ( 85.70 277.34 -9.00 0.59 S1) ; 25 + ( 87.16 278.29 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32.84 288.04 -52.75 0.07 S1) ; 93 + ( 33.06 283.55 -52.75 0.07 S1) ; 94 + ( 27.84 284.07 -52.75 0.07 S1) ; 95 + ( 27.33 288.11 -52.75 0.07 S1) ; 96 + ( 30.34 298.48 -51.89 0.07 S1) ; 97 + ( 33.50 297.30 -51.89 0.07 S1) ; 98 + ( 32.10 295.39 -51.89 0.07 S1) ; 99 + ( 30.78 297.15 -51.89 0.07 S1) ; 100 + ( 28.87 292.74 -50.95 0.07 S1) ; 101 + ( 32.84 294.95 -50.95 0.07 S1) ; 102 + ( 31.44 292.01 -51.22 0.07 S1) ; 103 + ( 31.44 291.49 -51.42 0.07 S1) ; 104 + ( 29.46 289.80 -51.42 0.07 S1) ; 105 + ( 27.70 289.43 -52.44 0.07 S1) ; 106 + ( 28.06 291.57 -52.75 0.07 S1) ; 107 + ( 27.99 292.38 -52.75 0.07 S1) ; 108 + ( 27.99 293.11 -52.75 0.07 S1) ; 109 + ( 28.72 294.29 -52.75 0.07 S1) ; 110 + ( 28.58 295.90 -52.75 0.07 S1) ; 111 + ( 29.16 296.86 -52.75 0.07 S1) ; 112 + ( 28.06 298.04 -52.75 0.07 S1) ; 113 + ( 32.84 296.71 -53.21 0.07 S1) ; 114 + ( 31.00 295.46 -53.21 0.07 S1) ; 115 + ( 30.56 294.80 -53.21 0.07 S1) ; 116 + ( 32.18 293.26 -53.21 0.07 S1) ; 117 + ( 31.88 290.10 -53.80 0.07 S1) ; 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4.43 0.59 S1) ; 24 + ( 41.53 286.41 6.07 0.59 S1) ; 25 + ( 42.12 285.77 7.20 0.59 S1) ; 26 + ( 43.29 287.05 8.52 0.59 S1) ; 27 + ( 42.99 289.27 10.10 0.59 S1) ; 28 + ( 41.53 291.18 10.28 0.59 S1) ; 29 + ( 39.48 292.14 10.40 0.59 S1) ; 30 + ( 37.44 292.77 10.90 0.59 S1) ; 31 + Normal + | + ( 29.83 322.36 -35.30 0.59 S1) ; 1, R-1-1-1-1-2-2-2-1-1-2 + ( 28.66 323.00 -35.30 0.59 S1) ; 2 + ( 26.91 324.59 -32.83 0.59 S1) ; 3 + ( 26.03 326.82 -32.15 0.59 S1) ; 4 + ( 25.74 328.41 -31.02 0.59 S1) ; 5 + ( 23.98 328.73 -29.05 0.59 S1) ; 6 + ( 24.57 329.68 -28.00 0.59 S1) ; 7 + ( 25.15 329.68 -24.70 0.59 S1) ; 8 + ( 24.57 331.27 -17.02 0.59 S1) ; 9 + ( 23.98 334.14 -15.82 0.59 S1) ; 10 + ( 23.40 334.77 -14.45 0.59 S1) ; 11 + ( 22.81 335.73 -12.72 0.59 S1) ; 12 + ( 22.81 337.00 -11.32 0.59 S1) ; 13 + ( 21.06 337.32 -9.82 0.59 S1) ; 14 + ( 19.30 337.95 -8.55 0.59 S1) ; 15 + ( 19.59 340.18 -6.15 0.59 S1) ; 16 + ( 20.47 342.09 -4.72 0.59 S1) ; 17 + ( 21.93 343.68 -3.92 0.59 S1) ; 18 + ( 20.47 344.64 -0.40 0.59 S1) ; 19 + ( 21.35 345.59 1.53 0.59 S1) ; 20 + ( 21.93 347.82 0.77 0.59 S1) ; 21 + ( 20.76 348.77 2.95 0.59 S1) ; 22 + ( 21.06 350.05 3.60 0.59 S1) ; 23 + ( 21.64 351.32 5.22 0.59 S1) ; 24 + ( 20.18 353.23 7.35 0.59 S1) ; 25 + ( 18.13 354.18 8.02 0.59 S1) ; 26 + ( 16.67 355.46 9.05 0.59 S1) ; 27 + ( 17.84 355.77 9.70 0.59 S1) ; 28 + ( 19.01 356.41 10.33 0.59 S1) ; 29 + ( + ( 20.76 355.14 7.45 0.59 S1) ; 1, R-1-1-1-1-2-2-2-1-1-2-1 + ( 21.64 353.55 7.22 0.59 S1) ; 2 + ( 22.81 352.27 6.22 0.59 S1) ; 3 + ( 24.57 351.00 5.68 0.59 S1) ; 4 + ( 25.15 349.41 5.37 0.59 S1) ; 5 + Normal + | + ( 18.72 358.32 12.55 0.59 S1) ; 1, R-1-1-1-1-2-2-2-1-1-2-2 + ( 20.18 359.27 13.23 0.59 S1) ; 2 + ( 21.93 360.55 13.67 0.59 S1) ; 3 + Low + ) ; End of split + ) ; End of split + | + ( 32.73 315.08 -57.37 0.59 S1) ; 1, R-1-1-1-1-2-2-2-1-2 + ( 33.90 315.72 -61.52 0.59 S1) ; 2 + ( 35.36 317.94 -62.08 0.59 S1) ; 3 + ( 36.53 320.17 -63.50 0.59 S1) ; 4 + ( 38.29 322.40 -66.92 0.59 S1) ; 5 + ( 38.87 324.94 -68.53 0.59 S1) ; 6 + ( 38.87 327.17 -70.38 0.59 S1) ; 7 + ( 38.29 328.45 -71.70 0.59 S1) ; 8 + ( 40.63 330.35 -72.10 0.59 S1) ; 9 + ( 42.09 331.95 -73.05 0.59 S1) ; 10 + ( 42.38 334.17 -75.78 0.59 S1) ; 11 + ( 43.26 335.45 -78.38 0.59 S1) ; 12 + ( 43.85 337.04 -81.05 0.59 S1) ; 13 + ( 45.31 338.31 -83.93 0.59 S1) ; 14 + ( 47.36 340.22 -85.68 0.59 S1) ; 15 + ( 49.70 343.72 -83.05 0.59 S1) ; 16 + ( 52.04 346.90 -89.82 0.59 S1) ; 17 + ( 53.79 348.81 -92.02 0.59 S1) ; 18 + ( 56.72 352.63 -91.40 0.59 S1) ; 19 + ( 58.18 355.49 -94.22 0.59 S1) ; 20 + ( 60.23 358.35 -96.20 0.59 S1) ; 21 + ( 61.40 361.85 -99.85 0.59 S1) ; 22 + ( 64.03 366.31 -99.85 0.59 S1) ; 23 + ( 65.20 369.81 -100.28 0.59 S1) ; 24 + ( 67.54 372.67 -100.92 0.59 S1) ; 25 + ( 69.90 376.52 -101.15 0.59 S1) ; 26 + ( 73.12 380.34 -103.05 0.59 S1) ; 27 + ( 78.09 382.56 -104.07 0.59 S1) ; 28 + ( 83.65 384.47 -104.27 0.59 S1) ; 29 + ( 85.99 385.11 -102.35 0.59 S1) ; 30 + ( 88.04 386.70 -105.63 0.59 S1) ; 31 + High + ) ; End 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28 + ( 42.24 1024.22 8.55 0.29 S1) ; 29 + ( 44.00 1025.49 9.15 0.29 S1) ; 30 + ( 45.17 1026.76 9.20 0.29 S1) ; 31 + ( 45.75 1027.40 9.20 0.29 S1) ; 32 + ( 46.92 1028.67 9.75 0.29 S1) ; 33 + ( 48.68 1029.63 10.07 0.29 S1) ; 34 + ( 49.85 1030.26 9.35 0.29 S1) ; 35 + ( 49.26 1031.86 9.15 0.29 S1) ; 36 + ( 50.72 1033.13 8.05 0.29 S1) ; 37 + ( 52.48 1033.76 5.95 0.29 S1) ; 38 + ( 52.77 1035.04 5.65 0.29 S1) ; 39 + ( 54.53 1036.31 4.97 0.29 S1) ; 40 + ( 56.57 1037.58 4.70 0.29 S1) ; 41 + ( 57.74 1036.63 4.30 0.29 S1) ; 42 + ( 59.21 1037.26 4.82 0.29 S1) ; 43 + ( 60.08 1038.54 6.75 0.29 S1) ; 44 + ( 61.84 1039.49 8.30 0.29 S1) ; 45 + ( 64.76 1041.72 8.65 0.29 S1) ; 46 + ( 67.10 1041.72 10.50 0.29 S1) ; 47 + ( 70.03 1042.67 10.80 0.29 S1) ; 48 + ( 72.95 1043.95 11.73 0.29 S1) ; 49 + ( 76.46 1046.17 12.47 0.29 S1) ; 50 + ( 77.34 1048.72 12.85 0.29 S1) ; 51 + ( 79.68 1050.63 12.98 0.29 S1) ; 52 + ( 81.14 1050.63 13.15 0.29 S1) ; 53 + ( 84.07 1052.86 13.38 0.29 S1) ; 54 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( 24.19 194.74 -45.63 0.88 S1) ; 1, R-1-1-1-2 + ( 23.02 196.02 -51.03 0.88 S1) ; 2 + ( 21.86 197.30 -55.13 0.88 S1) ; 3 + ( 20.10 197.94 -57.37 0.88 S1) ; 4 + ( 19.52 200.18 -60.15 0.88 S1) ; 5 + ( 17.77 203.06 -63.05 0.88 S1) ; 6 + ( 16.60 204.98 -63.80 0.88 S1) ; 7 + ( 13.98 207.55 -65.63 0.88 S1) ; 8 + ( 12.52 210.43 -66.00 0.88 S1) ; 9 + ( 10.77 212.35 -68.13 0.88 S1) ; 10 + ( 9.60 215.23 -69.05 0.88 S1) ; 11 + ( 9.60 217.15 -72.17 0.88 S1) ; 12 + ( 9.02 219.39 -74.13 0.88 S1) ; 13 + ( 7.26 222.92 -76.45 0.88 S1) ; 14 + ( 6.10 223.88 -82.30 0.88 S1) ; 15 + ( 4.64 227.40 -82.38 0.88 S1) ; 16 + ( 3.47 230.28 -86.13 0.88 S1) ; 17 + ( 1.43 231.88 -87.72 0.88 S1) ; 18 + ( 1.14 234.44 -89.95 0.88 S1) ; 19 + ( -2.66 235.40 -94.03 0.88 S1) ; 20 + Incomplete + ) ; End of split + | + ( 28.28 154.05 -49.40 1.17 S1) ; 1, R-1-1-2 + ( 30.32 153.09 -40.57 1.17 S1) ; 2 + ( 31.49 154.37 -36.33 1.17 S1) ; 3 + ( 33.53 155.33 -33.33 1.17 S1) ; 4 + ( 35.57 155.97 -32.40 1.17 S1) ; 5 + ( + ( 38.20 156.61 -31.20 1.17 S1) ; 1, R-1-1-2-1 + ( 40.53 156.93 -32.47 1.17 S1) ; 2 + ( 43.16 156.93 -33.95 1.17 S1) ; 3 + ( 46.66 159.17 -34.88 1.17 S1) ; 4 + ( + ( 47.54 160.77 -30.83 1.17 S1) ; 1, R-1-1-2-1-1 + ( 49.87 162.37 -31.40 1.17 S1) ; 2 + ( 51.91 164.30 -28.92 0.88 S1) ; 3 + ( 53.87 163.99 -26.80 0.88 S1) ; 4 + ( 56.78 165.59 -26.13 0.88 S1) ; 5 + ( 57.66 168.15 -25.70 0.88 S1) ; 6 + ( 59.70 170.71 -25.03 0.88 S1) ; 7 + ( 62.33 171.99 -23.57 0.88 S1) ; 8 + ( 64.08 173.91 -23.02 0.88 S1) ; 9 + ( 66.41 174.55 -21.32 0.88 S1) ; 10 + ( 67.87 175.19 -20.50 0.88 S1) ; 11 + ( + ( 71.08 176.16 -19.82 0.88 S1) ; 1, R-1-1-2-1-1-1 + ( 73.13 177.12 -19.17 0.88 S1) ; 2 + ( 74.58 179.36 -20.30 0.88 S1) ; 3 + ( 75.17 181.92 -24.35 0.88 S1) ; 4 + ( 76.04 184.48 -24.80 0.88 S1) ; 5 + ( 76.92 186.40 -27.97 0.88 S1) ; 6 + ( 77.21 188.32 -31.27 0.88 S1) ; 7 + ( 76.34 190.89 -31.58 0.88 S1) ; 8 + ( 75.46 193.13 -32.47 0.88 S1) ; 9 + ( 75.75 194.41 -32.60 0.88 S1) ; 10 + ( 76.63 196.01 -32.63 0.88 S1) ; 11 + ( 77.79 198.25 -32.65 0.88 S1) ; 12 + ( 78.96 198.89 -33.15 0.88 S1) ; 13 + ( 78.96 201.45 -33.33 0.88 S1) ; 14 + ( 79.55 203.69 -33.83 0.88 S1) ; 15 + ( 80.42 204.97 -33.97 0.88 S1) ; 16 + ( 81.30 206.26 -31.52 0.88 S1) ; 17 + ( 82.46 208.18 -29.45 0.88 S1) ; 18 + ( 82.17 211.06 -29.05 0.88 S1) ; 19 + ( 82.46 212.98 -32.92 0.88 S1) ; 20 + ( 83.05 215.86 -34.42 0.88 S1) ; 21 + ( 83.34 217.46 -35.40 0.88 S1) ; 22 + ( 85.09 219.38 -37.02 0.88 S1) ; 23 + ( 85.97 221.31 -37.80 0.88 S1) ; 24 + ( 86.84 224.19 -38.03 0.88 S1) ; 25 + ( 89.18 226.11 -38.90 0.88 S1) ; 26 + ( 90.63 228.35 -40.03 0.88 S1) ; 27 + ( + ( 94.39 229.66 -40.35 0.29 S1) ; 1, R-1-1-2-1-1-1-1 + ( 95.85 230.31 -42.53 0.29 S1) ; 2 + ( 97.02 231.59 -44.27 0.29 S1) ; 3 + ( 98.18 230.95 -44.85 0.29 S1) ; 4 + ( 100.23 231.27 -45.22 0.29 S1) ; 5 + ( 103.44 232.87 -45.42 0.29 S1) ; 6 + ( 105.19 234.47 -45.57 0.29 S1) ; 7 + ( 108.40 235.75 -45.92 0.29 S1) ; 8 + ( 110.73 236.71 -47.10 0.29 S1) ; 9 + ( 112.78 235.75 -47.25 0.29 S1) ; 10 + ( 116.28 236.39 -43.70 0.29 S1) ; 11 + ( 118.03 235.11 -42.92 0.29 S1) ; 12 + ( 119.78 234.47 -39.88 0.29 S1) ; 13 + ( 123.28 233.51 -38.17 0.29 S1) ; 14 + ( 125.61 231.91 -37.15 0.29 S1) ; 15 + ( 128.24 231.27 -34.88 0.29 S1) ; 16 + Normal + | + ( 93.58 232.43 -40.20 0.29 S1) ; 1, R-1-1-2-1-1-1-2 + ( 95.33 236.28 -40.40 0.29 S1) ; 2 + ( 98.54 237.88 -40.45 0.29 S1) ; 3 + ( 100.29 240.44 -40.45 0.29 S1) ; 4 + ( 104.67 241.40 -40.52 0.29 S1) ; 5 + ( 105.84 242.04 -41.22 0.29 S1) ; 6 + ( 106.13 243.96 -41.52 0.29 S1) ; 7 + ( 108.17 246.52 -41.80 0.29 S1) ; 8 + ( 110.50 248.12 -42.53 0.29 S1) ; 9 + ( 112.55 251.33 -43.27 0.29 S1) ; 10 + ( 114.01 254.21 -43.55 0.29 S1) ; 11 + ( 116.34 256.77 -44.32 0.29 S1) ; 12 + ( 118.97 256.77 -45.92 0.29 S1) ; 13 + ( 120.43 258.37 -47.63 0.29 S1) ; 14 + ( 122.18 260.29 -48.15 0.29 S1) ; 15 + ( 123.34 262.53 -50.68 0.29 S1) ; 16 + ( 126.26 264.78 -50.72 0.29 S1) ; 17 + ( 128.89 267.98 -50.77 0.29 S1) ; 18 + ( 130.64 269.58 -52.28 0.29 S1) ; 19 + ( 131.81 272.14 -53.97 0.29 S1) ; 20 + ( 134.43 273.74 -53.05 0.29 S1) ; 21 + ( 136.18 275.02 -53.92 0.29 S1) ; 22 + ( 138.23 276.94 -55.40 0.29 S1) ; 23 + ( 139.69 278.22 -57.63 0.29 S1) ; 24 + ( 140.27 280.79 -58.27 0.29 S1) ; 25 + Normal + ) ; End of split + | + ( 67.61 176.72 -18.13 0.29 S1) ; 1, R-1-1-2-1-1-2 + ( 65.86 178.64 -15.52 0.29 S1) ; 2 + ( 64.98 180.56 -14.88 0.29 S1) ; 3 + ( 64.69 183.12 -12.93 0.29 S1) ; 4 + ( 63.81 185.04 -11.25 0.29 S1) ; 5 + ( 62.36 185.68 -9.57 0.29 S1) ; 6 + ( 60.31 187.28 -8.38 0.29 S1) ; 7 + ( 61.48 190.17 -6.22 0.29 S1) ; 8 + ( 62.36 192.41 -4.20 0.29 S1) ; 9 + ( 62.65 194.33 -2.83 0.29 S1) ; 10 + ( 62.06 197.21 -2.65 0.29 S1) ; 11 + ( 61.77 200.09 -2.47 0.29 S1) ; 12 + ( 62.06 202.97 -2.75 0.29 S1) ; 13 + ( 61.77 205.22 -4.15 0.29 S1) ; 14 + ( 61.77 206.50 -5.77 0.29 S1) ; 15 + ( 60.90 209.06 -7.55 0.29 S1) ; 16 + ( 61.77 210.98 -7.95 0.29 S1) ; 17 + ( 62.06 212.26 -7.17 0.29 S1) ; 18 + ( 62.36 213.22 -3.78 0.29 S1) ; 19 + ( 60.90 215.78 -3.08 0.29 S1) ; 20 + ( 60.60 216.74 -2.53 0.29 S1) ; 21 + ( 60.90 218.99 0.05 0.29 S1) ; 22 + ( 62.06 221.87 0.50 0.29 S1) ; 23 + ( 62.94 223.15 0.60 0.29 S1) ; 24 + ( 64.11 224.43 -4.55 0.29 S1) ; 25 + ( 62.94 225.71 -5.92 0.29 S1) ; 26 + ( 63.52 226.35 -7.65 0.29 S1) ; 27 + ( 63.23 227.95 -10.07 0.29 S1) ; 28 + ( 63.23 229.87 -11.17 0.29 S1) ; 29 + ( 64.40 231.15 -11.27 0.29 S1) ; 30 + ( 67.32 233.07 -10.48 0.29 S1) ; 31 + Normal + ) ; End of split + | + ( 50.10 158.79 -34.88 1.17 S1) ; 1, R-1-1-2-1-2 + ( 53.02 159.11 -35.67 1.17 S1) ; 2 + ( 55.94 158.14 -36.27 1.17 S1) ; 3 + ( 58.85 157.82 -36.27 1.17 S1) ; 4 + ( 60.90 159.43 -37.38 1.17 S1) ; 5 + ( 63.81 160.39 -38.05 1.17 S1) ; 6 + ( 67.32 161.03 -38.25 1.17 S1) ; 7 + ( 71.11 159.75 -37.32 1.17 S1) ; 8 + ( 73.44 157.82 -39.67 1.17 S1) ; 9 + ( 75.20 157.18 -39.67 0.88 S1) ; 10 + ( 78.70 158.79 -37.77 0.88 S1) ; 11 + ( 80.74 159.75 -40.48 0.88 S1) ; 12 + ( 83.37 159.11 -40.52 0.88 S1) ; 13 + ( 85.70 157.82 -40.90 0.88 S1) ; 14 + ( 87.16 159.43 -41.12 0.88 S1) ; 15 + ( 90.66 160.71 -39.40 0.88 S1) ; 16 + ( 94.46 160.71 -38.80 0.88 S1) ; 17 + ( 97.96 161.35 -38.55 0.88 S1) ; 18 + ( 100.29 159.43 -38.40 0.88 S1) ; 19 + ( 103.21 161.67 -37.75 0.88 S1) ; 20 + ( 107.00 161.67 -37.50 0.88 S1) ; 21 + ( 110.51 161.03 -36.38 0.88 S1) ; 22 + ( 113.42 161.67 -35.02 0.88 S1) ; 23 + ( 115.47 161.99 -33.47 0.88 S1) ; 24 + ( 119.55 162.31 -32.72 0.88 S1) ; 25 + ( 121.59 161.35 -32.40 0.88 S1) ; 26 + ( 123.34 163.27 -33.92 0.88 S1) ; 27 + ( 125.68 164.23 -36.10 0.88 S1) ; 28 + ( 129.47 164.23 -36.20 0.88 S1) ; 29 + ( 131.27 165.67 -35.02 0.88 S1) ; 30 + ( 132.73 167.92 -33.92 0.58 S1) ; 31 + ( 136.23 168.24 -32.25 0.58 S1) ; 32 + ( 139.15 168.24 -32.02 0.58 S1) ; 33 + ( 140.61 169.20 -30.80 0.58 S1) ; 34 + ( 142.06 171.76 -30.47 0.58 S1) ; 35 + ( 143.09 174.68 -30.47 0.58 S1) ; 36 + ( 144.25 176.92 -34.88 0.58 S1) ; 37 + ( 147.17 178.20 -36.25 0.58 S1) ; 38 + ( 149.21 178.52 -37.05 0.58 S1) ; 39 + ( 150.67 179.81 -37.20 0.58 S1) ; 40 + ( 153.59 181.09 -38.97 0.58 S1) ; 41 + ( 157.09 182.05 -40.05 0.29 S1) ; 42 + ( 159.43 183.01 -40.85 0.29 S1) ; 43 + ( 160.30 184.29 -41.12 0.29 S1) ; 44 + ( 162.64 184.61 -42.77 0.29 S1) ; 45 + ( 166.14 184.29 -44.10 0.29 S1) ; 46 + ( 167.89 185.89 -45.20 0.29 S1) ; 47 + ( 168.18 187.81 -45.88 0.29 S1) ; 48 + Normal + ) ; End of split + | + ( 35.41 157.39 -26.50 1.17 S1) ; 1, R-1-1-2-2 + ( + ( 36.57 159.31 -24.17 0.88 S1) ; 1, R-1-1-2-2-1 + ( 36.86 161.55 -23.27 0.88 S1) ; 2 + ( 38.62 162.51 -19.97 0.88 S1) ; 3 + ( 39.49 162.83 -16.95 0.88 S1) ; 4 + ( 37.45 162.19 -15.75 0.88 S1) ; 5 + ( 38.03 163.15 -12.88 0.88 S1) ; 6 + ( 36.28 163.80 -10.33 0.58 S1) ; 7 + ( 35.11 163.80 -12.70 0.58 S1) ; 8 + ( 35.99 165.72 -8.97 0.58 S1) ; 9 + ( 36.86 167.00 -3.10 0.58 S1) ; 10 + ( 35.70 167.64 -1.50 0.58 S1) ; 11 + ( 36.57 170.20 1.55 0.58 S1) ; 12 + ( 35.99 170.20 5.37 0.58 S1) ; 13 + ( 35.11 169.88 10.52 0.58 S1) ; 14 + ( + ( 32.78 168.28 10.52 0.29 S1) ; 1, R-1-1-2-2-1-1 + ( 32.20 166.36 10.92 0.29 S1) ; 2 + ( 30.44 164.44 10.92 0.29 S1) ; 3 + Normal + | + ( 36.28 172.12 10.52 0.29 S1) ; 1, R-1-1-2-2-1-2 + ( 35.70 173.08 14.20 0.29 S1) ; 2 + ( 34.24 173.40 14.45 0.29 S1) ; 3 + ( 33.36 174.36 14.72 0.29 S1) ; 4 + ( 32.20 173.08 15.35 0.29 S1) ; 5 + Low + ) ; End of split + | + ( 33.07 159.31 -21.98 0.88 S1) ; 1, R-1-1-2-2-2 + ( 29.57 160.59 -21.00 0.88 S1) ; 2 + ( 27.23 162.83 -20.50 0.88 S1) ; 3 + ( 26.07 164.44 -18.92 0.88 S1) ; 4 + ( 24.02 166.36 -18.77 0.88 S1) ; 5 + ( 23.15 167.96 -16.63 0.88 S1) ; 6 + ( + ( 23.91 170.15 -13.77 0.88 S1) ; 1, R-1-1-2-2-2-1 + ( 22.46 171.11 -12.85 0.88 S1) ; 2 + ( 21.00 172.39 -11.63 0.88 S1) ; 3 + ( 21.87 174.63 -9.27 0.88 S1) ; 4 + ( 22.75 176.55 -6.65 0.88 S1) ; 5 + ( 21.29 178.80 -4.50 0.88 S1) ; 6 + ( 19.83 180.08 -3.70 0.88 S1) ; 7 + ( 20.12 182.64 -2.00 0.88 S1) ; 8 + ( 20.41 185.52 -1.90 0.88 S1) ; 9 + ( 19.83 187.44 -1.70 0.88 S1) ; 10 + ( 19.83 190.00 -1.55 0.88 S1) ; 11 + ( 20.41 192.56 -1.47 0.88 S1) ; 12 + ( 19.83 196.41 -1.40 0.88 S1) ; 13 + ( 18.95 200.57 -0.80 0.88 S1) ; 14 + ( 17.20 203.45 -0.80 0.88 S1) ; 15 + ( 15.74 204.73 -0.65 0.88 S1) ; 16 + ( 17.49 206.65 -0.20 0.88 S1) ; 17 + ( 18.66 209.22 0.17 0.88 S1) ; 18 + ( 18.66 211.78 0.45 0.88 S1) ; 19 + ( 17.49 214.02 1.38 0.88 S1) ; 20 + ( 17.79 216.58 1.80 0.88 S1) ; 21 + ( 17.49 219.14 1.90 0.88 S1) ; 22 + ( 18.08 221.38 1.20 0.88 S1) ; 23 + ( 19.25 222.99 0.05 0.88 S1) ; 24 + ( 18.08 225.55 -0.67 0.88 S1) ; 25 + ( 18.37 228.75 -2.33 0.88 S1) ; 26 + ( 17.79 231.63 -3.40 0.88 S1) ; 27 + ( 15.45 233.23 -3.50 0.88 S1) ; 28 + ( 12.83 235.15 -4.75 0.58 S1) ; 29 + ( 11.66 237.39 -5.53 0.58 S1) ; 30 + ( 11.37 240.28 -6.65 0.58 S1) ; 31 + ( 11.95 242.84 -6.73 0.58 S1) ; 32 + ( 11.37 246.68 -6.90 0.58 S1) ; 33 + ( 9.91 247.32 -6.90 0.58 S1) ; 34 + ( 6.70 248.60 -7.00 0.58 S1) ; 35 + ( 4.36 249.56 -7.53 0.58 S1) ; 36 + ( 2.90 248.92 -7.67 0.58 S1) ; 37 + Normal + | + ( 21.29 167.52 -15.80 0.88 S1) ; 1, R-1-1-2-2-2-2 + ( 19.25 168.16 -13.23 0.88 S1) ; 2 + ( 17.49 167.52 -11.10 0.88 S1) ; 3 + ( 16.33 166.24 -9.30 0.88 S1) ; 4 + ( 13.12 164.64 -9.02 0.88 S1) ; 5 + ( 9.91 164.00 -8.47 0.88 S1) ; 6 + ( 7.28 163.68 -8.42 0.88 S1) ; 7 + ( + ( 4.36 161.76 -10.63 0.58 S1) ; 1, R-1-1-2-2-2-2-1 + ( 2.32 161.12 -10.17 0.58 S1) ; 2 + ( 0.28 161.12 -8.82 0.58 S1) ; 3 + ( -0.01 161.44 -4.90 0.58 S1) ; 4 + ( -0.89 160.48 -1.02 0.58 S1) ; 5 + ( -2.06 160.16 -0.57 0.58 S1) ; 6 + ( -2.93 160.80 1.00 0.58 S1) ; 7 + ( -2.93 162.40 3.80 0.58 S1) ; 8 + ( -4.68 163.04 4.75 0.58 S1) ; 9 + ( -6.73 164.32 5.07 0.58 S1) ; 10 + ( -8.77 165.60 6.65 0.58 S1) ; 11 + ( -10.81 165.28 7.50 0.58 S1) ; 12 + ( -13.15 164.64 8.25 0.58 S1) ; 13 + ( -13.44 165.92 10.10 0.58 S1) ; 14 + ( -15.48 167.52 11.85 0.58 S1) ; 15 + ( -17.52 167.52 12.40 0.58 S1) ; 16 + ( -19.27 167.52 12.75 0.58 S1) ; 17 + ( -20.73 167.52 14.38 0.58 S1) ; 18 + ( -23.36 167.52 14.57 0.58 S1) ; 19 + ( -26.28 167.52 15.05 0.58 S1) ; 20 + Low + | + ( 5.24 165.60 -8.40 0.58 S1) ; 1, R-1-1-2-2-2-2-2 + ( 3.49 164.32 -6.52 0.58 S1) ; 2 + ( 2.03 164.64 -4.78 0.58 S1) ; 3 + ( 1.74 165.60 -3.42 0.58 S1) ; 4 + ( -1.18 165.28 -1.58 0.58 S1) ; 5 + ( -2.35 166.56 1.45 0.58 S1) ; 6 + ( -2.93 168.16 3.08 0.58 S1) ; 7 + ( -3.81 169.45 4.75 0.58 S1) ; 8 + ( -5.56 168.81 6.75 0.58 S1) ; 9 + ( -7.31 168.48 7.65 0.58 S1) ; 10 + ( -7.31 169.77 8.25 0.58 S1) ; 11 + ( -9.35 170.41 13.00 0.58 S1) ; 12 + ( -9.64 170.41 13.57 0.58 S1) ; 13 + ( + ( -11.98 169.45 9.35 0.58 S1) ; 1, R-1-1-2-2-2-2-2-1 + ( -14.60 169.13 8.05 0.58 S1) ; 2 + ( -16.36 169.45 6.05 0.58 S1) ; 3 + Normal + | + ( -9.64 172.33 13.57 0.58 S1) ; 1, R-1-1-2-2-2-2-2-2 + ( -9.94 174.25 12.20 0.58 S1) ; 2 + ( -10.81 174.89 11.13 0.58 S1) ; 3 + Low + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split 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; 23 + ( + ( 397.05 91.57 -116.32 0.58) ; 1, R-1-1-2-1-1-1 + ( 393.25 90.62 -116.72 0.58) ; 2 + ( 390.63 88.41 -116.85 0.29) ; 3 + ( 386.83 85.56 -117.00 0.29) ; 4 + ( 385.08 82.40 -117.40 0.29) ; 5 + ( 382.16 79.88 -117.75 0.29) ; 6 + ( 378.95 78.93 -118.20 0.29) ; 7 + ( 374.28 77.66 -118.42 0.29) ; 8 + ( 372.53 76.40 -119.07 0.29) ; 9 + ( 369.03 74.82 -119.12 0.29) ; 10 + ( 366.11 72.29 -120.13 0.29) ; 11 + High + | + ( 393.84 89.67 -119.82 0.29) ; 1, R-1-1-2-1-1-2 + ( 390.33 86.83 -122.40 0.29) ; 2 + ( 388.58 84.93 -126.30 0.29) ; 3 + ( 387.12 83.04 -130.55 0.29) ; 4 + ( 386.83 80.19 -133.00 0.29) ; 5 + ( 386.83 76.08 -134.63 0.29) ; 6 + ( 386.83 72.92 -137.05 0.29) ; 7 + ( 387.71 70.08 -140.55 0.29) ; 8 + ( 387.12 68.18 -140.68 0.29) ; 9 + ( 387.12 66.60 -142.35 0.29) ; 10 + ( 387.12 65.02 -146.57 0.29) ; 11 + ( 386.54 61.86 -149.65 0.29) ; 12 + ( 385.67 59.97 -153.20 0.29) ; 13 + ( 385.96 58.07 -156.22 0.29) ; 14 + ( 386.54 56.81 -158.93 0.29) ; 15 + ( 385.67 55.23 -159.97 0.29) ; 16 + ( 385.37 53.96 -161.02 0.29) ; 17 + ( 385.96 52.70 -161.65 0.29) ; 18 + ( 386.25 50.80 -161.70 0.29) ; 19 + ( 385.67 48.91 -161.70 0.29) ; 20 + ( 383.04 47.33 -162.10 0.29) ; 21 + Incomplete + ) ; End of split + | + ( 447.49 164.77 -84.05 0.58) ; 1, R-1-1-2-1-2 + ( 446.90 166.66 -84.52 0.58) ; 2 + ( 446.32 171.09 -85.63 0.58) ; 3 + ( 446.32 176.46 -87.22 0.58) ; 4 + ( 446.90 180.88 -89.88 0.58) ; 5 + ( 447.20 185.31 -93.05 0.58) ; 6 + ( 446.90 190.36 -96.10 0.58) ; 7 + ( 446.90 195.10 -98.80 0.58) ; 8 + ( 446.32 199.21 -99.50 0.58) ; 9 + ( 446.90 200.47 -100.90 0.58) ; 10 + ( 447.78 202.69 -102.92 0.58) ; 11 + ( 448.36 205.53 -105.47 0.58) ; 12 + ( 448.36 208.37 -108.45 0.58) ; 13 + ( 448.66 211.53 -112.82 0.58) ; 14 + ( 448.95 212.48 -116.50 0.58) ; 15 + ( 450.99 213.11 -117.02 0.58) ; 16 + ( 454.49 214.06 -120.75 0.58) ; 17 + ( 457.41 215.01 -122.35 0.58) ; 18 + ( 459.74 214.38 -124.57 0.58) ; 19 + ( 461.79 213.11 -126.53 0.58) ; 20 + ( 462.08 214.06 -131.70 0.58) ; 21 + ( 462.08 215.64 -131.80 0.58) ; 22 + ( 460.62 215.96 -133.63 0.58) ; 23 + ( 461.20 217.22 -136.72 0.58) ; 24 + ( 460.62 218.49 -138.52 0.58) ; 25 + ( 459.45 218.49 -140.95 0.58) ; 26 + ( 457.70 219.12 -142.35 0.58) ; 27 + High + ) ; End of split + | + ( 449.95 173.35 -48.30 0.58) ; 1, R-1-1-2-2 + ( 448.19 172.72 -48.47 0.58) ; 2 + ( 446.15 174.30 -48.78 0.58) ; 3 + ( 444.98 175.88 -49.47 0.58) ; 4 + ( 444.40 177.14 -51.10 0.58) ; 5 + ( 442.65 178.09 -53.00 0.58) ; 6 + ( 440.90 178.41 -53.70 0.58) ; 7 + ( 440.90 179.67 -53.90 0.58) ; 8 + ( 440.02 180.94 -54.07 0.58) ; 9 + ( 438.27 181.88 -54.78 0.58) ; 10 + ( 436.23 183.15 -55.35 0.58) ; 11 + ( 435.35 184.41 -56.77 0.58) ; 12 + ( 433.90 186.94 -57.80 0.58) ; 13 + ( 432.73 189.15 -60.25 0.58) ; 14 + ( 431.27 191.36 -63.97 0.58) ; 15 + ( 430.69 192.94 -66.20 0.58) ; 16 + ( 430.69 194.21 -66.60 0.58) ; 17 + ( 430.10 194.52 -66.60 0.58) ; 18 + ( 427.48 196.42 -67.68 0.58) ; 19 + ( 426.60 197.68 -68.70 0.58) ; 20 + ( 426.02 199.26 -69.40 0.58) ; 21 + ( 424.85 200.84 -71.68 0.58) ; 22 + ( 423.39 203.37 -71.92 0.58) ; 23 + ( 420.76 205.90 -73.32 0.58) ; 24 + ( 419.30 208.11 -74.55 0.58) ; 25 + ( 417.85 209.38 -76.30 0.58) ; 26 + ( 414.34 211.90 -77.90 0.58) ; 27 + ( 411.43 214.43 -79.53 0.58) ; 28 + ( 409.97 216.33 -81.50 0.58) ; 29 + ( 408.80 218.86 -83.95 0.58) ; 30 + ( 407.63 221.38 -86.40 0.58) ; 31 + ( 405.88 224.54 -89.03 0.58) ; 32 + ( 403.84 226.76 -92.60 0.58) ; 33 + ( 400.92 229.60 -93.85 0.58) ; 34 + ( 396.86 230.86 -94.65 0.58) ; 35 + ( 392.20 233.08 -95.10 0.58) ; 36 + ( 388.40 235.92 -96.32 0.58) ; 37 + ( 384.90 238.45 -97.28 0.58) ; 38 + ( 383.73 239.71 -97.70 0.58) ; 39 + ( 383.44 241.92 -98.10 0.58) ; 40 + ( 382.57 244.77 -98.32 0.58) ; 41 + ( 381.69 246.73 -99.15 0.58) ; 42 + ( 380.23 248.31 -99.25 0.58) ; 43 + ( 377.31 249.57 -99.43 0.58) ; 44 + ( 376.44 250.84 -99.60 0.58) ; 45 + ( 375.56 252.42 -99.78 0.58) ; 46 + ( 373.52 253.36 -100.28 0.58) ; 47 + ( 371.48 253.68 -101.00 0.58) ; 48 + ( 370.02 255.26 -101.75 0.58) ; 49 + ( 368.56 258.10 -102.57 0.58) ; 50 + ( 368.56 261.58 -104.32 0.58) ; 51 + Normal + ) ; End of split + ) ; End of split + | + ( 459.72 154.01 -25.90 2.33) ; 1, R-1-2 + ( 458.84 154.64 -22.27 0.58) ; 2 + ( 456.22 155.59 -18.48 0.58) ; 3 + ( 455.34 157.17 -16.30 0.58) ; 4 + ( 453.88 159.07 -15.70 0.58) ; 5 + ( 452.42 160.02 -15.38 0.58) ; 6 + + (Cross + (Color Orange) + (Name "Marker 3") + ( 458.55 155.28 -18.48 0.58) ; 1 + ( 457.38 155.91 -18.48 0.58) ; 2 + ( 454.76 156.22 -16.30 0.58) ; 3 + ( 456.51 157.49 -16.30 0.58) ; 4 + ( 456.22 158.12 -15.70 0.58) ; 5 + ( 455.63 159.38 -15.70 0.58) ; 6 + ( 454.76 157.17 -15.70 0.58) ; 7 + ( 454.17 158.12 -15.70 0.58) ; 8 + ( 453.01 158.75 -15.38 0.58) ; 9 + ( 453.01 160.96 -15.38 0.58) ; 10 + ) ; End of markers + ( + ( 451.84 158.12 -15.22 0.58) ; 1, R-1-2-1 + ( 450.09 156.22 -13.70 0.58) ; 2 + ( 447.75 156.22 -11.75 0.58) ; 3 + ( 446.59 156.22 -9.88 0.58) ; 4 + ( 445.71 158.12 -9.88 0.58) ; 5 + ( 444.54 158.75 -8.77 0.58) ; 6 + ( 444.25 158.75 -8.77 0.58) ; 7 + ( 442.79 158.12 -8.77 0.58) ; 8 + ( 440.75 158.12 -5.53 0.58) ; 9 + ( 439.29 158.75 -5.53 0.58) ; 10 + ( 436.96 158.12 -2.97 0.58) ; 11 + ( 435.79 157.80 -0.10 0.58) ; 12 + ( 434.33 158.75 3.50 0.58) ; 13 + ( 431.99 160.33 4.45 0.88) ; 14 + ( 429.37 161.28 6.57 0.88) ; 15 + ( 427.91 163.49 8.07 0.88) ; 16 + ( 424.99 165.07 8.97 0.88) ; 17 + ( 422.95 166.97 9.55 0.88) ; 18 + ( 420.91 167.28 11.25 0.88) ; 19 + ( 418.57 168.23 14.48 0.88) ; 20 + ( 417.70 169.50 17.40 0.88) ; 21 + ( 415.65 169.81 20.38 0.88) ; 22 + ( 414.19 169.18 21.05 0.88) ; 23 + ( 412.15 168.23 22.88 0.88) ; 24 + ( 410.69 168.55 24.00 0.58) ; 25 + ( 407.77 169.50 24.80 0.29) ; 26 + + (Cross + (Color Orange) + (Name "Marker 3") + ( 450.96 158.12 -13.70 0.58) ; 1 + ( 451.55 156.22 -13.70 0.58) ; 2 + ( 450.67 157.17 -13.70 0.58) ; 3 + ( 450.09 156.86 -13.70 0.58) ; 4 + ( 449.21 154.96 -13.70 0.58) ; 5 + ( 447.75 154.33 -11.75 0.58) ; 6 + ( 448.04 156.54 -11.75 0.58) ; 7 + ( 446.59 155.28 -9.88 0.58) ; 8 + ( 447.46 157.80 -9.88 0.58) ; 9 + ( 446.00 157.17 -9.88 0.58) ; 10 + ( 444.83 159.38 -8.77 0.58) ; 11 + ( 443.38 157.80 -8.77 0.58) ; 12 + ( 442.50 158.44 -8.77 0.58) ; 13 + ( 441.33 157.17 -5.53 0.58) ; 14 + ( 440.46 157.49 -5.53 0.58) ; 15 + ( 441.33 159.38 -5.53 0.58) ; 16 + ( 438.71 159.38 -3.22 0.58) ; 17 + ( 437.54 158.75 -3.22 0.58) ; 18 + ( 436.37 159.07 -0.10 0.58) ; 19 + ( 436.08 158.12 -0.10 0.58) ; 20 + ( 436.37 158.44 3.50 0.58) ; 21 + ( 433.45 160.65 4.45 0.88) ; 22 + ( 434.04 160.33 4.45 0.88) ; 23 + ( 431.70 160.33 4.45 0.88) ; 24 + ( 431.12 159.07 4.45 0.88) ; 25 + ( 432.29 159.07 5.53 0.88) ; 26 + ( 432.29 158.44 6.57 0.88) ; 27 + ( 432.58 160.33 6.57 0.88) ; 28 + ( 431.41 160.65 6.57 0.88) ; 29 + ( 429.95 161.60 6.57 0.88) ; 30 + ( 422.66 166.02 9.55 0.88) ; 31 + ( 425.28 166.65 9.55 0.88) ; 32 + ( 421.78 166.34 14.48 0.88) ; 33 + ( 419.45 169.18 20.38 0.88) ; 34 + ( 414.78 170.44 21.05 0.88) ; 35 + ( 414.78 167.28 22.88 0.88) ; 36 + ) ; End of markers + Low + | + ( 450.96 160.96 -15.70 0.58) ; 1, R-1-2-2 + ( 449.50 161.60 -18.70 0.58) ; 2 + ( 447.75 161.91 -21.32 0.58) ; 3 + ( 446.29 162.54 -21.98 0.58) ; 4 + ( 444.83 162.86 -22.65 0.58) ; 5 + ( 445.13 163.49 -22.80 0.58) ; 6 + ( 445.13 165.39 -22.97 0.58) ; 7 + ( 445.13 166.65 -24.02 0.58) ; 8 + ( 444.25 167.60 -25.52 0.58) ; 9 + ( 442.79 168.55 -26.82 0.58) ; 10 + ( 440.75 168.55 -27.27 0.58) ; 11 + ( 438.71 169.50 -27.42 0.58) ; 12 + ( 437.25 170.13 -28.37 0.58) ; 13 + ( 435.79 171.08 -27.65 0.58) ; 14 + ( 434.33 172.97 -26.50 0.58) ; 15 + ( 432.87 174.55 -26.10 0.58) ; 16 + ( 431.99 176.13 -26.67 0.58) ; 17 + ( 430.83 177.08 -27.27 0.58) ; 18 + ( 429.37 178.34 -27.42 0.58) ; 19 + ( 427.62 179.61 -27.57 0.58) ; 20 + ( 426.45 179.92 -27.73 0.58) ; 21 + ( 424.41 179.92 -27.75 0.58) ; 22 + ( 423.24 178.66 -27.88 0.58) ; 23 + ( 421.20 177.40 -29.25 0.58) ; 24 + ( 418.28 177.08 -30.87 0.58) ; 25 + ( 416.82 177.40 -33.30 0.58) ; 26 + ( 415.07 178.66 -34.38 0.58) ; 27 + ( 413.90 180.24 -35.22 0.58) ; 28 + ( 412.73 180.87 -35.35 0.58) ; 29 + ( 410.69 180.87 -35.57 0.58) ; 30 + ( 409.52 179.92 -36.13 0.58) ; 31 + ( 407.19 179.61 -36.82 0.58) ; 32 + ( 405.15 180.24 -38.57 0.58) ; 33 + ( 404.56 180.87 -38.85 0.58) ; 34 + ( 403.69 182.77 -40.10 0.58) ; 35 + ( 402.81 183.78 -40.32 0.58) ; 36 + ( 401.65 184.10 -40.45 0.58) ; 37 + ( 399.90 184.41 -40.60 0.58) ; 38 + ( 396.69 184.41 -42.25 0.88) ; 39 + ( 393.48 184.73 -43.35 0.88) ; 40 + ( 391.72 185.99 -43.72 0.88) ; 41 + ( 390.27 186.94 -45.70 0.58) ; 42 + ( 389.68 187.57 -47.10 0.58) ; 43 + ( 388.81 187.89 -48.75 0.58) ; 44 + ( 386.76 186.94 -50.25 0.88) ; 45 + ( 384.14 185.68 -51.08 0.88) ; 46 + ( 383.26 185.36 -51.93 0.58) ; 47 + ( 381.51 185.99 -52.10 0.58) ; 48 + ( 379.47 187.26 -53.30 0.58) ; 49 + ( 379.18 188.84 -54.20 0.58) ; 50 + ( 378.59 190.10 -55.22 0.58) ; 51 + ( 376.55 191.05 -55.83 0.58) ; 52 + ( 373.95 191.68 -55.90 0.58) ; 53 + ( 371.33 192.31 -56.32 0.58) ; 54 + ( 368.99 192.63 -56.32 0.58) ; 55 + ( 367.82 193.26 -57.05 0.58) ; 56 + ( 366.66 193.89 -57.37 0.58) ; 57 + ( 365.78 193.89 -57.45 0.58) ; 58 + ( 363.74 193.26 -58.15 0.58) ; 59 + ( 361.40 194.21 -59.80 0.58) ; 60 + ( 359.65 196.10 -60.52 0.58) ; 61 + ( 358.49 197.37 -60.30 0.58) ; 62 + ( 357.61 198.95 -59.47 0.58) ; 63 + ( 356.74 199.58 -59.42 0.58) ; 64 + ( 356.15 199.58 -59.02 0.58) ; 65 + ( 354.98 198.95 -58.90 0.58) ; 66 + ( 353.53 198.00 -58.07 0.58) ; 67 + ( 352.65 196.74 -56.52 0.58) ; 68 + ( 350.90 196.10 -56.30 0.58) ; 69 + ( 349.15 196.42 -55.13 0.58) ; 70 + ( 346.81 197.05 -54.63 0.58) ; 71 + ( 345.35 198.32 -54.55 0.58) ; 72 + ( 344.19 199.58 -54.25 0.58) ; 73 + ( 341.27 199.90 -54.13 0.58) ; 74 + ( 339.23 199.90 -54.97 0.58) ; 75 + ( 336.31 200.53 -56.70 0.58) ; 76 + ( 333.68 201.16 -57.17 0.58) ; 77 + ( 331.93 201.16 -57.30 0.58) ; 78 + ( 329.60 201.16 -57.50 0.58) ; 79 + ( 328.43 201.79 -57.77 0.58) ; 80 + ( 327.26 203.06 -57.85 0.58) ; 81 + ( 326.10 204.64 -57.95 0.58) ; 82 + ( 325.22 206.85 -57.95 0.58) ; 83 + ( 323.76 208.74 -58.02 0.58) ; 84 + ( 322.59 211.90 -58.05 0.58) ; 85 + ( 321.43 216.33 -58.35 0.58) ; 86 + ( 320.55 219.80 -58.67 0.58) ; 87 + ( 319.68 222.96 -58.97 0.58) ; 88 + ( 318.54 226.50 -59.13 0.58) ; 89 + ( 317.08 230.30 -60.13 0.58) ; 90 + ( 316.79 232.19 -60.20 0.58) ; 91 + ( 317.37 233.46 -60.32 0.58) ; 92 + ( 317.08 236.30 -60.45 0.58) ; 93 + ( 316.20 238.83 -60.95 0.58) ; 94 + ( 313.87 240.41 -61.17 0.58) ; 95 + ( 312.70 241.67 -61.85 0.58) ; 96 + ( 310.66 242.62 -62.25 0.58) ; 97 + ( 307.16 244.83 -62.75 0.29) ; 98 + ( 306.86 246.41 -64.07 0.29) ; 99 + ( 305.99 249.26 -64.95 0.29) ; 100 + ( 305.11 249.89 -65.95 0.29) ; 101 + ( 302.78 250.52 -66.00 0.29) ; 102 + ( 299.86 252.42 -66.38 0.29) ; 103 + ( 298.11 254.37 -66.82 0.29) ; 104 + ( 295.19 256.27 -67.50 0.29) ; 105 + ( 292.86 257.53 -67.80 0.29) ; 106 + ( 289.36 258.17 -68.17 0.29) ; 107 + ( 288.77 258.80 -68.22 0.29) ; 108 + Normal + ) ; End of split + ) ; End of split + | + ( 462.96 135.75 -21.45 0.58) ; 1, R-2 + ( 462.37 136.70 -18.85 0.58) ; 2 + ( 461.50 137.33 -15.22 0.58) ; 3 + ( 459.75 137.33 -13.45 0.58) ; 4 + ( 456.24 138.28 -12.65 0.88) ; 5 + ( 454.20 138.91 -12.38 0.58) ; 6 + ( 453.62 139.86 -8.63 0.58) ; 7 + ( 453.33 141.75 -8.13 0.58) ; 8 + ( 451.87 142.70 -7.97 0.58) ; 9 + ( 449.24 144.91 -5.95 0.58) ; 10 + ( 448.37 145.54 -4.30 0.88) ; 11 + + (Cross + (Color Orange) + (Name "Marker 3") + ( 458.29 137.01 -12.65 0.88) ; 1 + ( 455.08 137.64 -11.68 0.88) ; 2 + ( 452.16 141.12 -7.97 0.58) ; 3 + ( 453.03 139.86 -7.97 0.58) ; 4 + ( 450.70 142.70 -5.95 0.58) ; 5 + ( 450.12 143.33 -5.95 0.58) ; 6 + ( 451.87 144.60 -5.95 0.58) ; 7 + ( 449.53 146.18 -4.30 0.88) ; 8 + ( 447.49 144.60 -4.30 0.88) ; 9 + ) ; End of markers + ( + ( 448.66 146.81 -1.27 0.58) ; 1, R-2-1 + ( 448.95 146.81 0.40 0.58) ; 2 + ( 448.07 145.86 0.40 0.58) ; 3 + ( 447.20 145.86 2.13 0.58) ; 4 + ( 446.03 146.49 2.90 0.58) ; 5 + ( 446.03 147.12 4.85 0.58) ; 6 + ( 446.61 148.07 4.85 0.58) ; 7 + ( 448.66 149.34 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-80.40 0.91) ; 23 + ( -204.27 1103.77 -77.15 0.91) ; 24 + ( -207.68 1100.61 -79.85 0.91) ; 25 + ( -207.00 1099.75 -79.88 0.91) ; 26 + ) ; End of markers + Incomplete + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split +) ; End of tree diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/AMPA_ExcToExc.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/AMPA_ExcToExc.json new file mode 100755 index 00000000..c7585403 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/AMPA_ExcToExc.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 1.0, + "tau2": 3.0, + "erev": 0.0 +} diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/AMPA_ExcToInh.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/AMPA_ExcToInh.json new file mode 100755 index 00000000..43887996 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/AMPA_ExcToInh.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 0.1, + "tau2": 0.5, + "erev": 0.0 +} diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/GABA_InhToExc.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/GABA_InhToExc.json new file mode 100755 index 00000000..702ce9b4 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/GABA_InhToExc.json @@ -0,0 +1,7 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 2.7, + "tau2": 15.0, + "erev": -70.0 +} + diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/GABA_InhToInh.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/GABA_InhToInh.json new file mode 100755 index 00000000..ed4130ab --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/GABA_InhToInh.json @@ -0,0 +1,7 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 0.2, + "tau2": 8.0, + "erev": -70.0 +} + diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/instantaneousExc.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/instantaneousExc.json new file mode 100755 index 00000000..062e44e8 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/instantaneousExc.json @@ -0,0 +1,5 @@ +{ + "level_of_detail": "instantaneous", + "sign": 1 +} + diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/instantaneousInh.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/instantaneousInh.json new file mode 100755 index 00000000..1fc714cd --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/instantaneousInh.json @@ -0,0 +1,5 @@ +{ + "level_of_detail": "instantaneous", + "sign": -1 +} + diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/instanteneousExc.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/instanteneousExc.json new file mode 100755 index 00000000..062e44e8 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/instanteneousExc.json @@ -0,0 +1,5 @@ +{ + "level_of_detail": "instantaneous", + "sign": 1 +} + diff --git a/docs/tutorial/01_opt_external_models/components/synaptic_models/instanteneousInh.json b/docs/tutorial/01_opt_external_models/components/synaptic_models/instanteneousInh.json new file mode 100755 index 00000000..1fc714cd --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/synaptic_models/instanteneousInh.json @@ -0,0 +1,5 @@ +{ + "level_of_detail": "instantaneous", + "sign": -1 +} + diff --git a/docs/tutorial/01_opt_external_models/components/templates/Biophys.hoc b/docs/tutorial/01_opt_external_models/components/templates/Biophys.hoc new file mode 100644 index 00000000..5eb3717d --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/templates/Biophys.hoc @@ -0,0 +1,479 @@ +begintemplate Biophys + +public biophys + +proc biophys(){ + // Hay lab + if (strcmp($s2, "HL23PN1") == 0){ + forsec $o1.all { + insert pas + Ra = 100 + cm = 1 + e_pas = -80 + g_pas = 5e-5 + insert Ih + gbar_Ih = 1e-4 + } + forsec $o1.somatic { + insert SK + insert CaDynamics + insert Ca_LVA + insert Ca_HVA + insert K_T + insert K_P + insert Kv3_1 + insert NaTg + ek = -85 + ena = 50 + gamma_CaDynamics_E2 = 0.0005 + vshiftm_NaTg = 13 + vshifth_NaTg = 15 + slopem_NaTg = 7 + } + + forsec $o1.apical { + cm = 2 + } + forsec $o1.basal { + cm = 2 + } + $o1.distribute_channels("apic","gbar_Ih",2,-0.8696,3.6161,0.0,2.0870,$o1.soma.gbar_Ih) + + forsec $o1.axonal { + insert SK + insert Ca_LVA + insert Ca_HVA + insert K_T + insert K_P + insert Nap + insert Kv3_1 + insert NaTg + insert CaDynamics + ek = -85 + ena = 50 + vshifth_NaTg = 10 + slopem_NaTg = 9 + gamma_CaDynamics_E2 = 0.0005 + } + + $o1.distribute_channels("axon","decay_CaDynamics",0,1.000000,0.000000,0.000000,0.000000,79.1000000000) + $o1.distribute_channels("axon","gbar_SK",0,1.000000,0.000000,0.000000,0.000000,0.0346000000) + $o1.distribute_channels("axon","gbar_Ca_LVA",0,1.000000,0.000000,0.000000,0.000000,0.0002120000) + $o1.distribute_channels("axon","gbar_Ca_HVA",0,1.000000,0.000000,0.000000,0.000000,0.0000305000) + $o1.distribute_channels("axon","gbar_Kv3_1",0,1.000000,0.000000,0.000000,0.000000,0.8270000000) + $o1.distribute_channels("axon","gbar_K_T",0,1.000000,0.000000,0.000000,0.000000,0.0900000000) + $o1.distribute_channels("axon","gbar_K_P",0,1.000000,0.000000,0.000000,0.000000,0.2790000000) + $o1.distribute_channels("axon","gbar_Nap",0,1.000000,0.000000,0.000000,0.000000,0.0013800000) + $o1.distribute_channels("axon","gbar_NaTg",0,1.000000,0.000000,0.000000,0.000000,0.4860000000) + $o1.distribute_channels("soma","decay_CaDynamics",0,1.000000,0.000000,0.000000,0.000000,510.0000000000) + $o1.distribute_channels("soma","gbar_Ca_LVA",0,1.000000,0.000000,0.000000,0.000000,0.0000000000) + $o1.distribute_channels("soma","gbar_Ca_HVA",0,1.000000,0.000000,0.000000,0.000000,0.0005640000) + $o1.distribute_channels("soma","gbar_Kv3_1",0,1.000000,0.000000,0.000000,0.000000,0.0635000000) + $o1.distribute_channels("soma","gbar_SK",0,1.000000,0.000000,0.000000,0.000000,0.0000000000) + $o1.distribute_channels("soma","gbar_K_T",0,1.000000,0.000000,0.000000,0.000000,0.0508000000) + $o1.distribute_channels("soma","gbar_K_P",0,1.000000,0.000000,0.000000,0.000000,0.0001870000) + $o1.distribute_channels("soma","gbar_NaTg",0,1.000000,0.000000,0.000000,0.000000,0.2390000000) + } + if (strcmp($s2, "HL23MN1") == 0){ + forsec $o1.all { + insert pas + Ra = 133.859093627 + cm = 1 + e_pas = -84.73198852539 + g_pas = 2.25e-5 + insert Ih + gbar_Ih = 1.2e-4 + } + forsec $o1.somatic { + insert SK + insert CaDynamics + insert Ca_LVA + insert Ca_HVA + insert K_T + insert K_P + insert Kv3_1 + insert NaTg + ek = -85 + ena = 50 + gamma_CaDynamics_E2 = 0.0005 + vshiftm_NaTg = 13 + vshifth_NaTg = 15 + slopem_NaTg = 7 + } + + forsec $o1.axonal { + insert SK + insert Ca_LVA + insert Ca_HVA + insert K_T + insert K_P + insert Nap + insert Kv3_1 + insert NaTg + insert CaDynamics + ek = -85 + ena = 50 + vshifth_NaTg = 10 + slopem_NaTg = 9 + gamma_CaDynamics_E2 = 0.0005 + } + + $o1.distribute_channels("axon","decay_CaDynamics",0,1.000000,0.000000,0.000000,0.000000,20.0000000000) + $o1.distribute_channels("axon","gbar_SK",0,1.000000,0.000000,0.000000,0.000000,0.0002220000) + $o1.distribute_channels("axon","gbar_Ca_LVA",0,1.000000,0.000000,0.000000,0.000000,0.0329000000) + $o1.distribute_channels("axon","gbar_Ca_HVA",0,1.000000,0.000000,0.000000,0.000000,0.0006260000) + $o1.distribute_channels("axon","gbar_Kv3_1",0,1.000000,0.000000,0.000000,0.000000,0.9390000000) + $o1.distribute_channels("axon","gbar_K_T",0,1.000000,0.000000,0.000000,0.000000,0.0679000000) + $o1.distribute_channels("axon","gbar_K_P",0,1.000000,0.000000,0.000000,0.000000,0.0356000000) + $o1.distribute_channels("axon","gbar_Nap",0,1.000000,0.000000,0.000000,0.000000,0.0068700000) + $o1.distribute_channels("axon","gbar_NaTg",0,1.000000,0.000000,0.000000,0.000000,1.0000000000) + $o1.distribute_channels("soma","decay_CaDynamics",0,1.000000,0.000000,0.000000,0.000000,21.7000000000) + $o1.distribute_channels("soma","gbar_Ca_LVA",0,1.000000,0.000000,0.000000,0.000000,0.0003520000) + $o1.distribute_channels("soma","gbar_Ca_HVA",0,1.000000,0.000000,0.000000,0.000000,0.0073200000) + $o1.distribute_channels("soma","gbar_Kv3_1",0,1.000000,0.000000,0.000000,0.000000,0.7110000000) + $o1.distribute_channels("soma","gbar_SK",0,1.000000,0.000000,0.000000,0.000000,0.0003360000) + $o1.distribute_channels("soma","gbar_K_T",0,1.000000,0.000000,0.000000,0.000000,0.0402000000) + $o1.distribute_channels("soma","gbar_K_P",0,1.000000,0.000000,0.000000,0.000000,0.0197000000) + $o1.distribute_channels("soma","gbar_NaTg",0,1.000000,0.000000,0.000000,0.000000,0.2620000000) + } + // Allen ID 626170661 + if (strcmp($s2, "HL23BN1") == 0){ + forsec $o1.all { + insert pas + insert Ih + Ra = 100 + cm = 2 + e_pas = -83.85905159083714 + g_pas = 0.00013276715388485768 + gbar_Ih = 4.0286355749662496e-05 + } + + forsec $o1.somatic { + insert NaTg + insert Nap + insert K_P + insert K_T + insert Kv3_1 + insert Im + insert SK + insert Ca_HVA + insert Ca_LVA + insert CaDynamics + ek = -85 + ena = 50 + gbar_NaTg = 0.4999668678729633 + vshiftm_NaTg = 0 + vshifth_NaTg = 10 + slopem_NaTg = 9 + slopeh_NaTg = 6 + gbar_Nap = 0.0005315169096865464 + gbar_K_P = 0.01713385484501281 + gbar_K_T = 0.0639088696383371 + gbar_Kv3_1 = 2.9998799002509644 + vshift_Kv3_1 = 0 + gbar_Im = 0.0032868613326996676 + gbar_SK = 2.9074984128489475e-06 + gbar_Ca_HVA = 0.0005963580477926297 + gbar_Ca_LVA = 0.04068051219830349 + gamma_CaDynamics = 0.0005 + decay_CaDynamics = 477.52284259122615 + } + + forsec $o1.axonal { + insert NaTg + insert Nap + insert K_P + insert K_T + insert Kv3_1 + insert Im + insert SK + insert Ca_HVA + insert Ca_LVA + insert CaDynamics + ek = -85 + ena = 50 + gbar_NaTg = 0.06497772772932814 + vshiftm_NaTg = 0 + vshifth_NaTg = 10 + slopem_NaTg = 9 + slopeh_NaTg = 6 + gbar_Nap = 0.0004227598793218445 + gbar_K_P = 0.01348368226376475 + gbar_K_T = 0.053430438020626844 + gbar_Kv3_1 = 0.894628255182179 + vshift_Kv3_1 = 0 + gbar_Im = 0.0032433844107431437 + gbar_SK = 0.07857804528458547 + gbar_Ca_HVA = 6.976985998831502e-05 + gbar_Ca_LVA = 0.0026754479897141132 + gamma_CaDynamics = 0.0005 + decay_CaDynamics = 21.082144088397 + } + + // forsec $o1.basal { + // insert NaTg + // insert Nap + // insert K_P + // insert K_T + // insert Kv3_1 + // insert Im + // insert SK + // insert Ca_HVA + // insert Ca_LVA + // insert CaDynamics + // } + } + if (strcmp($s2, "HL23VN1") == 0){ + forsec $o1.all { + insert pas + insert Ih + Ra = 100 + cm = 2 + e_pas = -81.64099928563398 + g_pas = 5.640407332265837e-05 + gbar_Ih = 7.937538666539732e-05 + shift1_Ih = 117.54696999889302 + shift2_Ih = 3.460797910265395 + shift3_Ih = -49.69676238671805 + shift4_Ih = 58.27114333073568 + shift5_Ih = 86.63729662000578 + shift6_Ih = 281.49007535744454 + } + + // forsec $o1.somatic { + // insert NaTg + // insert Nap + // insert K_P + // insert K_T + // insert Kv3_1 + // insert SK + // insert Im + // insert Ca_HVA + // insert Ca_LVA + // insert CaDynamics + // ek = -85 + // ena = 50 + // gbar_NaTg = 0.11474659824436195 + // vshiftm_NaTg = 13 + // vshifth_NaTg = 15 + // slopem_NaTg = 7 + // slopeh_NaTg = 6 + // gbar_Nap = 0.00047681727902698157 + // gbar_K_P = 0.055957749184639055 + // gbar_K_T = 0.09996880674736379 + // gbar_Kv3_1 = 0.3788563067742772 + // vshift_Kv3_1 = 0 + // gbar_SK = 0.010560196535257488 + // gbar_Im = 0.00034301521472746954 + // gbar_Ca_HVA = 9.018554611915845e-05 + // gbar_Ca_LVA = 0.0541616355609249 + // gamma_CaDynamics = 0.0005 + // decay_CaDynamics = 20.08804065219189 + // } + // + // forsec $o1.axonal { + // insert NaTg + // insert Nap + // insert K_P + // insert K_T + // insert Kv3_1 + // insert SK + // insert Im + // insert Ca_HVA + // insert Ca_LVA + // insert CaDynamics + // ek = -85 + // ena = 50 + // gbar_NaTg = 0.22734023408743798 + // vshiftm_NaTg = 0 + // vshifth_NaTg = 10 + // slopem_NaTg = 9 + // slopeh_NaTg = 6 + // gbar_Nap = 0.006541044566273077 + // gbar_K_P = 0.00421820957878577 + // gbar_K_T = 0.037928705443776765 + // gbar_Kv3_1 = 0.3196733124005582 + // vshift_Kv3_1 = 0 + // gbar_SK = 0.3804947699033776 + // gbar_Im = 0.00017885126314180088 + // gbar_Ca_HVA = 0.00024547511717151337 + // gbar_Ca_LVA = 0.04299507068179599 + // gamma_CaDynamics = 0.0005 + // decay_CaDynamics = 25.229936800309066 + // } + } + if (strcmp($s2, "HL4PN1") == 0){ + forsec $o1.all { + insert pas + insert Ih + Ra = 100 + cm = 1 + e_pas = -92.20988602082443 + g_pas = 2.496019938803455e-05 + gbar_Ih = 3.923425401401172e-05 + shift1_Ih = 224.81524109817627 + shift2_Ih = 15.549405048102342 + shift3_Ih = 51.004970487634765 + shift4_Ih = 34.58507073356797 + shift5_Ih = 92.39602997332304 + shift6_Ih = 38.603169245772854 + } + forsec $o1.apical { + cm = 1.75 + } + forsec $o1.basal { + cm = 1.75 + } + $o1.distribute_channels("apic","gbar_Ih",4,-0.8696,0.0031,0.0,2.0870,$o1.soma.gbar_Ih) + $o1.distribute_channels("dend","gbar_Ih",4,-0.8696,0.0031,0.0,2.0870,$o1.soma.gbar_Ih) + $o1.distribute_channels("axon","gbar_Ih",4,-0.8696,0.0031,0.0,2.0870,$o1.soma.gbar_Ih) + + forsec $o1.somatic { + insert NaTg + insert Nap + insert K_P + insert K_T + insert Kv3_1 + insert SK + insert Im + insert Ca_HVA + insert Ca_LVA + insert CaDynamics + ek = -85 + ena = 50 + gbar_NaTg = 0.45162251179862545 + vshiftm_NaTg = 0 + vshifth_NaTg = 10 + slopem_NaTg = 9 + slopeh_NaTg = 6 + gbar_Nap = 0.0015800320564183347 + gbar_K_P = 0.09857157410308227 + gbar_K_T = 0.00022137399530109607 + gbar_Kv3_1 = 0.277107453007135 + vshift_Kv3_1 = 0 + gbar_SK = 0.3566622775104453 + gbar_Im = 0.0002993987916613973 + gbar_Ca_HVA = 1.1501967667916403e-08 + gbar_Ca_LVA = 0.004357365263342773 + gamma_CaDynamics = 0.0005 + decay_CaDynamics = 435.1670601322996 + } + + forsec $o1.axonal { + insert NaTg + insert Nap + insert K_P + insert K_T + insert Kv3_1 + insert SK + insert Im + insert Ca_HVA + insert Ca_LVA + insert CaDynamics + ek = -85 + ena = 50 + gbar_NaTg = 0.032257472930859954 + vshiftm_NaTg = 0 + vshifth_NaTg = 10 + slopem_NaTg = 9 + slopeh_NaTg = 6 + gbar_Nap = 0.0006895353184589968 + gbar_K_P = 0.08697896141698211 + gbar_K_T = 0.04046361467898627 + gbar_Kv3_1 = 0.7886318280246498 + vshift_Kv3_1 = 0 + gbar_SK = 0.00157409272881637 + gbar_Im = 0.00022399456776878458 + gbar_Ca_HVA = 1.1973335394266724e-05 + gbar_Ca_LVA = 0.06346837616023718 + gamma_CaDynamics = 0.0005 + decay_CaDynamics = 134.26668638182747 + } + } + + if (strcmp($s2, "HL5PN1") == 0){ + forsec $o1.all { + insert pas + insert Ih + Ra = 100 + cm = 0.9 + e_pas = -88.05900508094575 + g_pas = 1.082202245504852e-06 + gbar_Ih = 1.737556184697235e-05 + shift1_Ih = 144.76545935424588 + shift2_Ih = 14.382865335237211 + shift3_Ih = -28.179477866349245 + shift4_Ih = 99.18311385307702 + shift5_Ih = 16.42000098505615 + shift6_Ih = 26.699880497099517 + } + $o1.distribute_channels("apic","gbar_Ih",5,0.5,24,950,-285,$o1.soma.gbar_Ih) + $o1.distribute_channels("dend","gbar_Ih",5,0.5,24,950,-285,$o1.soma.gbar_Ih) + $o1.distribute_channels("axon","gbar_Ih",5,0.5,24,950,-285,$o1.soma.gbar_Ih) + + forsec $o1.somatic { + insert NaTg + insert Nap + insert K_P + insert K_T + insert Kv3_1 + insert SK + insert Im + insert Ca_HVA + insert Ca_LVA + insert CaDynamics + ek = -85 + ena = 50 + gbar_NaTg = 0.2868981428991897 + vshiftm_NaTg = 0 + vshifth_NaTg = 10 + slopem_NaTg = 9 + slopeh_NaTg = 6 + gbar_Nap = 0.0005928501660669944 + gbar_K_P = 0.630442269948701 + gbar_K_T = 0.7534865864927842 + gbar_Kv3_1 = 0.4595039202419757 + vshift_Kv3_1 = 0 + gbar_SK = 1.0429209585502295 + gbar_Im = 0.00043980640140570256 + gbar_Ca_HVA = 4.590237477358571e-06 + gbar_Ca_LVA = 0.004922323805158312 + gamma_CaDynamics = 0.0005 + decay_CaDynamics = 424.5119756515562 + } + + forsec $o1.axonal { + insert NaTg + insert Nap + insert K_P + insert K_T + insert Kv3_1 + insert SK + insert Im + insert Ca_HVA + insert Ca_LVA + insert CaDynamics + ek = -85 + ena = 50 + gbar_NaTg = 0.0020817650993738114 + vshiftm_NaTg = 0 + vshifth_NaTg = 10 + slopem_NaTg = 9 + slopeh_NaTg = 6 + gbar_Nap = 0.0030669969011709527 + gbar_K_P = 0.10640803057846296 + gbar_K_T = 0.6887341085216281 + gbar_Kv3_1 = 1.4723945773672162 + vshift_Kv3_1 = 0 + gbar_SK = 0.018607376942477858 + gbar_Im = 5.422509445418661e-06 + gbar_Ca_HVA = 6.899898046124077e-06 + gbar_Ca_LVA = 0.007967297071061932 + gamma_CaDynamics = 0.0005 + decay_CaDynamics = 447.5127627121552 + } + } +} + + +endtemplate Biophys diff --git a/docs/tutorial/01_opt_external_models/components/templates/HNTemplate.hoc b/docs/tutorial/01_opt_external_models/components/templates/HNTemplate.hoc new file mode 100644 index 00000000..edf40ba0 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/components/templates/HNTemplate.hoc @@ -0,0 +1,506 @@ +begintemplate HNTemplate + +public init, delete_axon, insertChannel, distribute, geom_nseg, geom_nsec +public set_parameters, locateSites, getLongestBranch, distribute_channels +public distributeSyn, connect2target, createArtificialSyn, InitConnections +public initRand, indexSections, cell_name +public all, apical, basal, somatic, axonal, nSecAll, nSecSoma, nSecApical, nSecBasal, cell_name +public soma, dend, apic, axon, myelin, rList, cons, synlist, OUprocess, fih, rslist +public position, xoffset, yoffset, zoffset, xloc, yloc, zloc, diamval + +objref synlist, cons, rList, rd1, sref, this, segCounts, sf, OUprocess, fih, rslist +objref all, somatic, apical, axonal, basal +strdef tstr, cell_name + +proc init() { localobj nl,imprt, bp + all = new SectionList() + somatic = new SectionList() + basal = new SectionList() + apical = new SectionList() + axonal = new SectionList() + forall delete_section() + cell_name = $s2 + stimThreshold = 0 + synlist = new List() + OUprocess = new List() + rslist = new List() + cons = new List() + + //load morphology + sf = new StringFunctions() + if (sf.substr($s1, ".asc") != -1){ + nl = new Import3d_Neurolucida3() + } else{ + nl = new Import3d_SWC_read() + } + nl.quiet = 1 + nl.input($s1) + imprt = new Import3d_GUI(nl, 0) + imprt.instantiate(this) + + geom_nseg() + // delete_axon(3,1.75,1,1) + delete_axon() // For plotting network shape plot only + bp = new Biophys() + bp.biophys(this, $s2) + initRand(1005) + forsec this.all { + if(diam == 0){ + diam = 1 + printf("Error : Morphology problem with section [%s] 0 diam \n", secname()) + } + } + lengthA = 0 + lengthB = 0 + forsec "apic" { + lengthA = lengthA + L + } + forsec "dend" { + lengthB = lengthB + L + } + pA = lengthA/(lengthA + lengthB) +} + +create soma[1], dend[1], apic[1], axon[1], myelin[1] + +proc geom_nseg() {local nSec, L1, L2, D1, D2, nSeg1, nSeg2 + soma area(.5) // make sure diam reflects 3d points + nSec = 0 + forsec all { + nseg = 1 + 2*int(L/40) + nSec = nSec + 1 + } + + nSecAll = nSec + nSec = 0 + forsec somatic { nSec = nSec + 1} + nSecSoma = nSec + nSec = 0 + forsec apical { nSec = nSec + 1} + nSecApical= nSec + nSec = 0 + forsec basal { nSec = nSec + 1} + nSecBasal = nSec + nSec = 0 + forsec axonal { nSec = nSec + 1} + nSecAxonalOrig = nSecAxonal = nSec +} + +proc delete_axon(){ + nsec = 0 + forsec axonal{ + nsec = nsec + 1 + } + if (nsec == 0){ + ais_diams1 = 1 + ais_diams2 = 1 + } else if (nsec == 1){ + ais_diams1 = axon[0].diam + ais_diams2 = axon[0].diam + } else { + ais_diams1 = axon[0].diam + ais_diams2 = axon[0].diam + soma distance(0,0.5) + forsec axonal{ + if (distance(1,0.5) > 60){ + ais_diams2 = diam + break + } + } + } + + forsec axonal{delete_section()} + create axon[2] + access axon[0]{ + L= 30 + nseg = 1//+2*int(L/10) + diam = ais_diams1 + all.append() + axonal.append() + } + access axon[1]{ + L= 30 + nseg = 1//+2*int(L/10) + diam = ais_diams2 + all.append() + axonal.append() + } + + nSecAxonal = 2 + connect axon[0](0), soma[0](1) + connect axon[1](0), axon[0](1) + access soma +} + +func getLongestBranch(){local maxL,d localobj distallist,sref + sprint(tstr,"%s distance()",$s1) + execute(tstr,this) + + if(0==strcmp($s1,"axon")){ + sprint(tstr,"%s[0] distance(1)",$s1) + execute(tstr,this) + } + + maxL = 0 + d = 0 + distallist = new SectionList() + forsec $s1 { + sref = new SectionRef() + if (sref.nchild==0) distallist.append() + } + forsec distallist{ + d = distance(1) + if(maxL= d0) { + siteX = (site-d0)/(d1-d0) + secNum = i + vv = new Vector() + ll.append(vv.append(secNum,siteX)) + } + i = i+1 + } + return ll +} + +proc distribute_channels() {local dist,val,base,maxLength + base = $8 + soma distance() + maxLength = getLongestBranch($s1) + + forsec $s1 { + if(0==strcmp($s2,"Ra")){ + Ra = $8 + } else { + for(x) { + if (($3==3) || ($3==4) || ($3==5)) { + dist = distance(x) + } else { + dist = distance(x)/maxLength + } + val = calculate_distribution($3,dist,$4,$5,$6,$7,$8) + sprint(tstr,"%s(%-5.10f) = %-5.10f",$s2,x,val) + execute(tstr) + } + } + } +} + +// $1 is the distribution type: +// 0 linear, 1 sigmoid, 2 exponential +// 3 step for absolute distance (in microns) +// 4 exponential with absolute distance (compatible with bluepyopt) +// 5 sigmoidal with absolute distance +func calculate_distribution() {local value + if ($1==0) {value = $3 + $2*$4} + if ($1==1) {value = $3 + ($4/(1+exp(($2-$5)/$6)))} + if ($1==2) {value = $3 + $6*exp($4*($2-$5))} + if ($1==3) { + if (($2 > $5) && ($2 < $6)) { + value = $3 + } else { + value = $4 + } + } + if ($1==4) {value = $3 + $6*exp($4*($2-$5))} + if ($1==5) {value = $3 + ($4/(1+exp(($2-$5)/$6)))} + value = value*$7 + return value +} + +// shift cell location - Random +// proc position(){ local i +// xoffset = rd1.repick()*600 - 300 //int($1/10)*60 - 300// Option for grid placement +// yoffset = rd1.repick()*600 - 300 //($1%10)*60 - 300// Option for grid placement +// zoffset = 0 +// // Find first coordinate to first subtract it to a somatic coordinate of (0,0,0) +// soma x = x3d(0) +// soma y = y3d(0) +// soma z = z3d(0) +// soma for i=0,n3d()-1 { +// // fprint("\nCoordinates: (%g,%g,%g)",x3d(i), y3d(i), z3d(i)) +// xloc = x3d(i)+xoffset-x +// yloc = y3d(i)+yoffset-y +// zloc = z3d(i)+zoffset-z +// diamval = diam3d(i) +// pt3dchange(i, xloc, yloc, zloc, diamval) +// } +// define_shape() // shifts all the other sections in line with somatic section +// } + +// Shift Cell Location - Manual + +proc position() { local i + soma for i = 0, n3d()-1 { // ith point of number of 3d locations stored in the currently accessed section + pt3dchange(i, $1+x3d(i), $2+y3d(i), $3+z3d(i), diam3d(i)) // Change the ith 3-d point info, Returns the x/y/z coordinate of the ith point in the 3-d list of the currently accessed section. + } + define_shape() +} +// pt3dstyle() // use if visualization gets wee-woo + + +//======================================================================================== +//============================== synapse related functions =============================== +//======================================================================================== + +// $1 some number that is function of the cell index +// Have each cell with unique sites, but certain portion of inputs identical to root cell +proc initRand() { + rList = new List() //for stochastic synapses + rd1 = new Random($1) // unique to this cell + rd1.uniform(0,1) +} + +proc connect2target() { //$o1 target point process, $o2 returned NetCon + soma $o2 = new NetCon(&v(1), $o1) + $o2.threshold = -30 +} + +double siteVec[2] +proc createArtificialSyn() {local sitenum,OUni,i localobj sl + strdef treename,cmd2 + PYR = $2 + for(i=0;i<10;i+=1){ + + if (i<5){ + treename = "dend" + relpos = 0.5 + sl = locateSites(treename,relpos*getLongestBranch(treename)) + siteVec[0] = sl.o[i].x[0] + siteVec[1] = sl.o[i].x[1] + }else{ + if (PYR==0){ + // If not a pyramidal cell, stop at 5 OU processes + break + } + treename = "apic" + relpos = (i-5)*0.2 + 0.1 // [0.1, 0.3, 0.5, 0.7, 0.9] + sl = locateSites(treename,relpos*getLongestBranch(treename)) + sitenum = int((sl.count()-1)) + siteVec[0] = sl.o[sitenum].x[0] + siteVec[1] = sl.o[sitenum].x[1] + } + + sprint(cmd2,"access %s[siteVec[0]]",treename) + execute(cmd2,this) + + sprint(cmd2,"%s[siteVec[0]] sref = new SectionRef()",treename) + execute(cmd2,this) + + sref { + OUprocess.append(new Gfluct2(siteVec[1])) + OUni = OUprocess.count()-1 // OU object index + + // Set OU parameters + OUprocess.o[OUni].E_e = 0// time constant of excitatory conductance + OUprocess.o[OUni].E_i = -80 // time constant of inhibitory conductance + OUprocess.o[OUni].g_e0 = 0.001*exp(relpos) //60 // average excitatory conductance + OUprocess.o[OUni].g_i0 = 0.005*exp(relpos) // average inhibitory conductance + OUprocess.o[OUni].tau_e = 65 // time constant of excitatory conductance + OUprocess.o[OUni].tau_i = 20 // time constant of inhibitory conductance + OUprocess.o[OUni].std_e = 0.001*exp(relpos) // standard dev of excitatory conductance + OUprocess.o[OUni].std_i = 0.005*exp(relpos) // standard dev of inhibitory conductance + // OUprocess.o[OUni].new_seed($1*10+i) // This appears to not be threadsafe + rslist.append(new Random($1*10+i)) + rslist.o[OUni].normal(0,1) + OUprocess.o[OUni].noiseFromRandom(rslist.o[OUni]) + } + // fprint("\nOU Process #%g Set",$1*10+i) + } +} + +obfunc distributeSyn() {local sitenum,syni,preconi,i localobj sl,nilstim + if ((strcmp($o1.cell_name, "HL23PN1") == 0)){ + EsynConductance = 0.00047 + NsynE = 5 + NsynI = 0 + if ((strcmp(cell_name, "HL23MN1") == 0)){ + EsynConductance = 0.00032 + NsynE = 8 + NsynI = 0 + } + } + if (strcmp($o1.cell_name, "HL23MN1") == 0){ + IsynConductance = 0.028 + NsynE = 0 + NsynI = 12 + } + strdef treename,cmd2 + for(i=0;i<(NsynE+NsynI);i+=1){ + if (rd1.repick() $5) && ($2 < $6)) { + value = $3 + } else { + value = $4 + } + } + value = value*$7 + return value +} + +// $s1 section +func getLongestBranch(){local maxL,d localobj distallist,sref + sprint(tstr,"%s distance()",$s1) + execute(tstr,this) + + if(0==strcmp($s1,"axon")){ + sprint(tstr,"%s[0] distance(1)",$s1) + execute(tstr,this) + } + + maxL = 0 + d = 0 + distallist = new SectionList() + forsec $s1 { + sref = new SectionRef() + if (sref.nchild==0) distallist.append() + } + forsec distallist{ + d = distance(1) + if(maxL= d0) { + siteX = (site-d0)/(d1-d0) + secNum = i + vv = new Vector() + ll.append(vv.append(secNum,siteX)) + } + i = i+1 + } + return ll +} + +func getAbsSecIndex(){ local nAbsInd, index localobj str,strObj + strObj = new StringFunctions() + str = new String() + nAbsInd = 0 + index = 0 + if(strObj.substr($s1, "soma") > 0) { + strObj.tail($s1, "soma", str.s) + if(sscanf(str.s, "%*c%d", &index) < 0) { + index = 0 + } + nAbsInd = index + }else if (strObj.substr($s1, "axon") >0) { + strObj.tail($s1, "axon", str.s) + if(sscanf(str.s, "%*c%d", &index) < 0) { + index = 0 + } + nAbsInd = nSecSoma + index + }else if (strObj.substr($s1, "dend") >0) { + strObj.tail($s1, "dend", str.s) + if(sscanf(str.s, "%*c%d", &index) < 0) { + index = 0 + } + nAbsInd = nSecSoma + nSecAxonalOrig + index + }else if (strObj.substr($s1, "apic") > 0) { + strObj.tail($s1, "apic", str.s) + if(sscanf(str.s, "%*c%d", &index) < 0) { + index = 0 + } + nAbsInd = nSecSoma + nSecAxonalOrig + nSecBasal + index + } + return nAbsInd +} + + +endtemplate L5PCtemplate \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/config.L5.json b/docs/tutorial/01_opt_external_models/config.L5.json new file mode 100755 index 00000000..0c218e8d --- /dev/null +++ b/docs/tutorial/01_opt_external_models/config.L5.json @@ -0,0 +1,70 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_L5", + "$NETWORK_DIR": "$BASE_DIR/network_L5", + "$COMPONENTS_DIR": "$BASE_DIR/components" + }, + "target_simulator": "NEURON", + "run": { + "tstart": 0.0, + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15.0, + "nsteps_block": 5000 + }, + "conditions": { + "celsius": 34.0, + "v_init": -80.0 + }, + "inputs": { + "virt_exc_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$BASE_DIR/inputs/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + "components": { + "filter_models_dir": "$COMPONENTS_DIR/filter_models", + "templates_dir": ["$COMPONENTS_DIR/templates/L5PCbiophys4.hoc", "$COMPONENTS_DIR/templates/L5PCtemplate.hoc"], + "biophysical_neuron_models_dir": "$COMPONENTS_DIR/biophysical_neuron_models", + "mechanisms_dir": "$COMPONENTS_DIR/mechanisms", + "synaptic_models_dir": "$COMPONENTS_DIR/synaptic_models", + "morphologies_dir": "$COMPONENTS_DIR/morphologies", + "point_neuron_models_dir": "$COMPONENTS_DIR/point_neuron_models" + }, + "output": { + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv" + }, + "reports": { + "v_report": { + "variable_name": "v", + "cells": "all", + "module": "membrane_report", + "sections": "soma" + } + }, + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/L5_nodes.h5", + "node_types_file": "$NETWORK_DIR/L5_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": "$NETWORK_DIR/virt_exc_node_types.csv" + } + ], + "edges": [ + { + "edge_types_file": "$NETWORK_DIR/virt_exc_L5_edge_types.csv", + "edges_file": "$NETWORK_DIR/virt_exc_L5_edges.h5" + } + ] + } +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/config.L5_updated.json b/docs/tutorial/01_opt_external_models/config.L5_updated.json new file mode 100755 index 00000000..aabab93b --- /dev/null +++ b/docs/tutorial/01_opt_external_models/config.L5_updated.json @@ -0,0 +1,75 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_L5_updated", + "$NETWORK_DIR": "$BASE_DIR/network_L5_updated", + "$COMPONENTS_DIR": "$BASE_DIR/components" + }, + "target_simulator": "NEURON", + "run": { + "tstart": 0.0, + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15.0, + "nsteps_block": 5000 + }, + "conditions": { + "celsius": 34.0, + "v_init": -80.0 + }, + "inputs": { + "virt_exc_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$BASE_DIR/inputs/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + "components": { + "filter_models_dir": "$COMPONENTS_DIR/filter_models", + "templates_dir": [ + "$COMPONENTS_DIR/templates/L5PCbiophys4.hoc", + "$COMPONENTS_DIR/templates/Biophys.hoc", + "$COMPONENTS_DIR/templates/HNTemplate.hoc", + "$COMPONENTS_DIR/templates/L5PCtemplate.hoc" + ], + "biophysical_neuron_models_dir": "$COMPONENTS_DIR/biophysical_neuron_models", + "mechanisms_dir": "$COMPONENTS_DIR/mechanisms", + "synaptic_models_dir": "$COMPONENTS_DIR/synaptic_models", + "morphologies_dir": "$COMPONENTS_DIR/morphologies", + "point_neuron_models_dir": "$COMPONENTS_DIR/point_neuron_models" + }, + "output": { + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv" + }, + "reports": { + "v_report": { + "variable_name": "v", + "cells": "all", + "module": "membrane_report", + "sections": "soma" + } + }, + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/L5_nodes.h5", + "node_types_file": "$NETWORK_DIR/L5_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": "$NETWORK_DIR/virt_exc_node_types.csv" + } + ], + "edges": [ + { + "edge_types_file": "$NETWORK_DIR/virt_exc_L5_edge_types.csv", + "edges_file": "$NETWORK_DIR/virt_exc_L5_edges.h5" + } + ] + } +} \ No newline at end of file diff --git a/docs/tutorial/01_opt_external_models/config.added_channels.json b/docs/tutorial/01_opt_external_models/config.added_channels.json new file mode 100755 index 00000000..e12a460c --- /dev/null +++ b/docs/tutorial/01_opt_external_models/config.added_channels.json @@ -0,0 +1,70 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_added_channels", + "$NETWORK_DIR": "$BASE_DIR/network_added_channels", + "$COMPONENTS_DIR": "$BASE_DIR/components" + }, + "target_simulator": "NEURON", + "run": { + "tstart": 0.0, + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15.0, + "nsteps_block": 5000 + }, + "conditions": { + "celsius": 34.0, + "v_init": -80.0 + }, + "inputs": { + "virt_exc_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$BASE_DIR/inputs/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + "components": { + "filter_models_dir": "$COMPONENTS_DIR/filter_models", + "templates_dir": "$COMPONENTS_DIR/templates", + "biophysical_neuron_models_dir": "$COMPONENTS_DIR/biophysical_neuron_models", + "mechanisms_dir": ["$COMPONENTS_DIR/mechanisms", "$COMPONENTS_DIR/dLGN_mechanisms"], + "synaptic_models_dir": "$COMPONENTS_DIR/synaptic_models", + "morphologies_dir": "$COMPONENTS_DIR/morphologies", + "point_neuron_models_dir": "$COMPONENTS_DIR/point_neuron_models" + }, + "output": { + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv" + }, + "reports": { + "v_report": { + "variable_name": "v", + "cells": "all", + "module": "membrane_report", + "sections": "soma" + } + }, + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/L5_nodes.h5", + "node_types_file": "$NETWORK_DIR/L5_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": 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-930.27 -23.00 1.52) ; 4 + ( -292.70 -930.39 -23.00 1.52) ; 5 + ( -295.48 -930.65 -23.00 1.52) ; 6 + ( -296.37 -931.28 -23.00 1.52) ; 7 + ( + ( -298.65 -930.65 -23.00 1.01) ; 1, R-1-1-2-1-1-1 + ( -298.78 -930.52 -23.00 1.01) ; 2 + ( -300.42 -929.51 -23.00 1.01) ; 3 + ( -302.95 -928.24 -23.00 1.01) ; 4 + ( -303.08 -928.24 -23.00 1.01) ; 5 + ( -303.46 -928.12 -23.00 1.01) ; 6 + ( + ( -304.85 -926.72 -23.00 1.01) ; 1, R-1-1-2-1-1-1-1 + ( -305.74 -925.46 -23.00 1.01) ; 2 + ( -307.38 -923.81 -23.00 1.01) ; 3 + ( -309.66 -923.56 -23.00 1.01) ; 4 + ( -312.45 -923.56 -23.00 1.01) ; 5 + ( -313.97 -922.80 -23.00 1.01) ; 6 + ( -314.22 -922.67 -23.00 1.01) ; 7 + ( -315.23 -921.91 -23.00 1.01) ; 8 + ( -315.86 -921.41 -23.00 1.01) ; 9 + Normal + | + ( -306.37 -928.24 -23.00 1.01) ; 1, R-1-1-2-1-1-1-2 + ( -309.03 -928.50 -23.00 1.01) ; 2 + ( -309.41 -928.50 -23.00 1.01) ; 3 + ( -312.19 -928.75 -23.00 1.01) ; 4 + ( -314.09 -928.62 -23.00 1.01) ; 5 + ( -314.22 -928.62 -23.00 1.01) ; 6 + ( -314.35 -928.50 -23.00 1.01) ; 7 + ( -316.88 -927.48 -23.00 1.01) ; 8 + ( -319.16 -925.71 -23.00 1.01) ; 9 + ( + ( -320.04 -925.58 -23.00 1.01) ; 1, R-1-1-2-1-1-1-2-1 + ( -320.17 -925.58 -23.00 1.01) ; 2 + ( -321.69 -924.45 -23.00 1.01) ; 3 + ( -322.32 -923.43 -23.00 1.01) ; 4 + ( -323.46 -922.80 -23.00 1.01) ; 5 + Normal + | + ( -318.78 -924.19 -23.00 1.01) ; 1, R-1-1-2-1-1-1-2-2 + ( -318.78 -921.66 -23.00 1.01) ; 2 + ( -318.78 -921.03 -23.00 1.01) ; 3 + Normal + ) ; End of split + ) ; End of split + | + ( -300.04 -932.04 -23.00 1.65) ; 1, R-1-1-2-1-1-2 + ( -302.95 -933.18 -23.00 1.65) ; 2 + ( + ( -304.75 -932.40 -23.00 1.01) ; 1, R-1-1-2-1-1-2-1 + ( -307.79 -931.77 -23.00 1.01) ; 2 + ( -310.20 -931.77 -23.00 1.01) ; 3 + ( -313.62 -931.64 -23.00 1.01) ; 4 + ( -316.78 -931.90 -23.00 1.01) ; 5 + ( + ( -321.46 -928.98 -23.00 1.01) ; 1, R-1-1-2-1-1-2-1-1 + ( -321.59 -928.98 -23.00 1.01) ; 2 + ( -324.37 -926.58 -23.00 0.89) ; 3 + ( -326.40 -925.06 -23.00 0.89) ; 4 + ( -327.54 -924.17 -23.00 0.89) ; 5 + ( + ( -331.21 -924.68 -23.00 0.89) ; 1, R-1-1-2-1-1-2-1-1-1 + ( -332.48 -923.29 -25.75 0.89) ; 2 + ( -332.73 -923.16 -25.75 0.89) ; 3 + ( -334.25 -921.52 -25.75 0.89) ; 4 + ( -334.25 -921.26 -25.75 0.89) ; 5 + ( -334.88 -918.48 -25.75 0.89) ; 6 + ( -334.88 -918.10 -25.75 0.89) ; 7 + ( -335.01 -916.71 -25.75 0.89) ; 8 + Normal + | + ( -328.81 -920.88 -23.00 0.89) ; 1, R-1-1-2-1-1-2-1-1-2 + ( -328.55 -919.62 -23.00 0.89) ; 2 + ( -328.43 -919.36 -23.00 0.89) ; 3 + ( -327.54 -917.59 -23.00 0.89) ; 4 + ( -327.41 -917.46 -23.00 0.89) ; 5 + ( -323.74 -916.07 -23.00 0.89) ; 6 + ( -322.22 -916.07 -23.00 0.89) ; 7 + Normal + ) ; End of split + | + ( -319.06 -932.28 -23.00 0.89) ; 1, R-1-1-2-1-1-2-1-2 + ( -323.36 -932.28 -23.75 0.89) ; 2 + ( -326.40 -932.53 -24.50 0.89) ; 3 + ( -329.56 -933.41 -24.50 0.89) ; 4 + ( -331.08 -934.81 -24.50 0.89) ; 5 + ( -331.21 -934.81 -24.50 0.89) ; 6 + ( -332.86 -935.44 -24.50 0.89) ; 7 + ( -333.11 -935.44 -24.50 0.89) ; 8 + ( -335.64 -936.07 -24.50 0.89) ; 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R-1-1-2-1-1-2-2-1 + ( -303.74 -939.11 -23.50 1.01) ; 2 + ( -303.62 -941.39 -23.50 1.01) ; 3 + ( -304.12 -941.39 -23.50 0.89) ; 4 + ( + ( -306.53 -942.28 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-1 + ( -308.17 -943.16 -23.50 0.89) ; 2 + ( -309.69 -944.93 -23.50 0.89) ; 3 + ( -311.34 -946.71 -23.50 0.89) ; 4 + ( -312.35 -949.11 -23.50 0.89) ; 5 + ( + ( -311.08 -951.26 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-1-1 + ( -311.08 -951.39 -23.50 0.89) ; 2 + ( -311.08 -951.77 -23.50 0.89) ; 3 + Normal + | + ( -314.50 -950.50 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-1-2 + ( -316.27 -953.79 -23.50 0.89) ; 2 + ( -316.40 -953.92 -23.50 0.89) ; 3 + ( -317.67 -954.93 -23.50 0.89) ; 4 + ( -317.67 -955.06 -23.50 0.89) ; 5 + ( -317.79 -955.19 -23.50 0.89) ; 6 + ( + ( -318.30 -958.22 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-1-2-1 + ( -318.17 -958.60 -23.50 0.89) ; 2 + ( -317.67 -960.63 -23.50 0.89) ; 3 + ( -317.67 -960.88 -23.50 0.89) ; 4 + ( -317.67 -961.39 -23.50 0.89) ; 5 + ( -317.67 -961.52 -23.50 0.89) ; 6 + ( -317.79 -961.77 -23.50 0.89) ; 7 + Normal + | + ( -320.32 -955.69 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-1-2-2 + ( -320.70 -955.69 -23.50 0.89) ; 2 + ( -322.10 -956.71 -23.50 0.89) ; 3 + ( -322.48 -957.09 -23.50 0.89) ; 4 + Normal + ) ; End of split + ) ; End of split + | + ( -304.50 -943.79 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-2 + ( + ( -302.35 -945.69 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-2-1 + ( -301.34 -947.59 -23.50 0.89) ; 2 + ( -301.21 -947.72 -23.50 0.89) ; 3 + ( -299.31 -950.00 -23.50 0.89) ; 4 + ( -298.93 -951.77 -23.50 0.89) ; 5 + ( -298.55 -951.77 -23.50 0.89) ; 6 + ( -297.54 -952.02 -23.50 0.89) ; 7 + ( -297.41 -952.15 -23.50 0.89) ; 8 + ( -296.91 -952.40 -23.50 0.89) ; 9 + ( + ( -293.36 -954.05 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-2-1-1 + ( -293.24 -954.17 -23.50 0.89) ; 2 + ( -291.08 -954.30 -23.50 0.89) ; 3 + ( -290.83 -954.05 -23.50 0.89) ; 4 + ( -290.70 -954.05 -23.50 0.89) ; 5 + ( -289.06 -952.65 -23.50 0.89) ; 6 + ( -288.43 -952.40 -23.50 0.89) ; 7 + ( -286.65 -951.26 -23.50 0.89) ; 8 + ( -286.02 -951.26 -23.50 0.89) ; 9 + ( -284.63 -950.76 -23.50 0.89) ; 10 + ( -284.12 -950.76 -23.50 0.89) ; 11 + Normal + | + ( -295.89 -957.34 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-2-1-2 + ( -295.77 -958.60 -23.50 0.89) ; 2 + ( -295.64 -958.86 -23.50 0.89) ; 3 + ( -295.64 -958.98 -23.50 0.89) ; 4 + ( -295.64 -960.25 -23.50 0.89) ; 5 + ( -295.64 -960.50 -23.50 0.89) ; 6 + ( -295.64 -960.50 -23.50 0.89) ; 7 + Normal + ) ; End of split + | + ( -305.01 -947.97 -23.50 0.89) ; 1, R-1-1-2-1-1-2-2-1-2-2 + ( -306.27 -950.12 -23.50 0.89) ; 2 + ( -307.67 -952.15 -23.50 0.89) ; 3 + ( + ( -309.69 -955.57 -23.50 0.76) ; 1, R-1-1-2-1-1-2-2-1-2-2-1 + ( -310.58 -957.34 -23.50 0.76) ; 2 + ( -310.70 -957.59 -23.50 0.76) ; 3 + ( -311.72 -959.11 -23.50 0.76) ; 4 + ( -311.97 -959.24 -23.50 0.76) ; 5 + ( -313.36 -960.76 -23.50 0.76) ; 6 + ( -313.74 -962.02 -23.50 0.76) ; 7 + ( -313.74 -962.40 -23.50 0.76) ; 8 + ( -314.12 -963.16 -23.50 0.76) ; 9 + Normal + | + ( -305.77 -954.30 -23.50 0.76) ; 1, R-1-1-2-1-1-2-2-1-2-2-2 + ( -305.77 -954.43 -23.50 0.76) ; 2 + ( -304.25 -955.44 -23.50 0.76) ; 3 + ( -303.99 -955.69 -23.50 0.76) ; 4 + ( -303.49 -956.33 -23.50 0.76) ; 5 + ( -302.98 -956.71 -23.50 0.76) ; 6 + ( -302.86 -956.96 -23.50 0.76) ; 7 + ( -301.59 -957.59 -23.50 0.76) ; 8 + ( -301.34 -957.59 -23.50 0.76) ; 9 + ( -300.45 -958.98 -23.50 0.76) ; 10 + ( -300.20 -959.36 -23.50 0.76) ; 11 + ( -299.94 -960.00 -23.50 0.76) ; 12 + ( -299.69 -960.25 -23.50 0.76) ; 13 + ( -299.18 -960.88 -23.50 0.76) ; 14 + ( -298.93 -961.52 -23.50 0.76) ; 15 + ( -298.43 -961.77 -23.50 0.76) ; 16 + ( -298.05 -961.90 -23.50 0.76) ; 17 + ( -297.54 -962.15 -23.50 0.76) ; 18 + ( -297.29 -962.15 -23.50 0.76) ; 19 + ( -297.16 -962.28 -23.50 0.76) ; 20 + ( -296.91 -962.28 -23.50 0.76) ; 21 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -307.16 -934.93 -23.50 1.65) ; 1, R-1-1-2-1-1-2-2-2 + ( -310.32 -935.82 -23.50 1.65) ; 2 + ( + ( -312.86 -935.69 -23.50 1.14) ; 1, R-1-1-2-1-1-2-2-2-1 + ( -314.88 -935.31 -22.25 1.14) ; 2 + ( -319.18 -936.07 -22.25 1.01) ; 3 + ( -320.83 -936.45 -22.25 1.01) ; 4 + ( -321.30 -936.68 -22.25 1.01) ; 5 + ( + ( -323.10 -935.11 -22.25 1.01) ; 1, R-1-1-2-1-1-2-2-2-1-1 + ( -325.54 -934.26 -22.25 1.01) ; 2 + ( -328.67 -935.64 -21.75 1.01) ; 3 + Normal + | + ( -324.34 -939.21 -22.75 1.01) ; 1, R-1-1-2-1-1-2-2-2-1-2 + ( -324.59 -939.21 -22.75 1.01) ; 2 + ( -330.41 -940.23 -22.75 1.01) ; 3 + Normal + ) ; End of split + | + ( -311.84 -938.10 -23.50 1.27) ; 1, R-1-1-2-1-1-2-2-2-2 + ( -311.97 -937.97 -23.50 1.27) ; 2 + ( + ( -313.11 -940.25 -23.50 1.01) ; 1, R-1-1-2-1-1-2-2-2-2-1 + ( -313.74 -941.64 -23.50 1.01) ; 2 + ( -315.77 -944.93 -23.50 1.01) ; 3 + ( -318.17 -947.09 -23.50 1.01) ; 4 + ( + ( -321.97 -950.76 -23.50 1.01) ; 1, R-1-1-2-1-1-2-2-2-2-1-1 + ( -322.98 -952.40 -23.50 1.01) ; 2 + ( -323.62 -953.67 -23.50 1.01) ; 3 + ( + ( -323.74 -955.95 -23.50 0.76) ; 1, R-1-1-2-1-1-2-2-2-2-1-1-1 + ( -322.98 -958.35 -23.50 0.76) ; 2 + ( -322.86 -959.49 -23.50 0.76) ; 3 + ( -322.86 -959.74 -23.50 0.76) ; 4 + ( -322.22 -961.52 -23.50 0.76) ; 5 + Normal + | + ( -325.51 -955.19 -23.50 0.76) ; 1, R-1-1-2-1-1-2-2-2-2-1-1-2 + ( -326.02 -956.71 -23.50 0.76) ; 2 + ( -325.77 -957.97 -23.50 0.76) ; 3 + ( -325.64 -958.10 -23.50 0.76) ; 4 + ( -325.64 -959.36 -23.50 0.76) ; 5 + ( -325.77 -959.62 -23.50 0.76) ; 6 + ( -325.89 -959.74 -23.50 0.76) ; 7 + Normal + ) ; End of split + ) ; End of split + | + ( -313.99 -938.86 -23.50 1.27) ; 1, R-1-1-2-1-1-2-2-2-2-2 + ( -315.64 -939.74 -23.50 1.27) ; 2 + ( -317.92 -941.39 -23.50 1.27) ; 3 + ( -319.69 -942.65 -23.50 1.27) ; 4 + ( -321.21 -944.30 -23.50 1.27) ; 5 + ( -321.34 -944.43 -23.50 1.27) ; 6 + ( -323.74 -944.68 -23.50 1.27) ; 7 + ( -323.87 -944.68 -23.50 1.27) ; 8 + ( + ( -330.20 -948.10 -23.75 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-1 + ( -330.45 -948.10 -23.75 0.63) ; 2 + ( + ( -332.86 -951.39 -23.75 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-1-1 + ( -335.13 -953.03 -23.75 0.63) ; 2 + ( -335.39 -953.03 -23.75 0.63) ; 3 + ( -337.92 -953.54 -23.75 0.63) ; 4 + ( -338.05 -953.54 -23.75 0.63) ; 5 + ( -341.21 -954.17 -23.75 0.63) ; 6 + ( -341.46 -954.05 -23.75 0.63) ; 7 + ( -343.62 -954.17 -23.75 0.63) ; 8 + ( -344.00 -954.17 -23.75 0.63) ; 9 + ( -344.00 -954.30 -23.75 0.63) ; 10 + ( -345.13 -954.43 -23.75 0.63) ; 11 + ( -345.39 -954.43 -23.75 0.63) ; 12 + Normal + | + ( -333.99 -949.11 -23.75 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-1-2 + ( -334.25 -949.24 -23.75 0.63) ; 2 + ( -335.77 -949.24 -23.75 0.63) ; 3 + ( -336.27 -949.24 -23.75 0.63) ; 4 + ( -338.81 -949.24 -23.75 0.63) ; 5 + ( -338.93 -949.36 -23.75 0.63) ; 6 + ( -340.83 -950.00 -23.75 0.63) ; 7 + ( -340.96 -950.00 -23.75 0.63) ; 8 + ( -343.49 -950.88 -23.75 0.63) ; 9 + ( -343.87 -951.01 -23.75 0.63) ; 10 + ( -346.40 -950.76 -23.75 0.63) ; 11 + ( -346.78 -950.76 -23.75 0.63) ; 12 + ( -349.44 -951.90 -23.75 0.63) ; 13 + ( -349.69 -952.28 -23.75 0.63) ; 14 + ( -351.08 -953.41 -23.75 0.63) ; 15 + ( -351.34 -953.79 -23.75 0.63) ; 16 + ( -352.48 -954.43 -23.75 0.63) ; 17 + ( -352.73 -954.55 -23.75 0.63) ; 18 + ( -352.86 -954.55 -23.75 0.63) ; 19 + ( -353.24 -954.68 -23.75 0.63) ; 20 + Normal + ) ; End of split + | + ( -326.78 -944.17 -23.50 1.01) ; 1, R-1-1-2-1-1-2-2-2-2-2-2 + ( -329.94 -943.67 -23.50 1.01) ; 2 + ( -332.35 -943.03 -23.50 1.01) ; 3 + ( -332.48 -943.03 -23.50 1.01) ; 4 + ( -334.63 -943.03 -23.50 1.01) ; 5 + ( -334.88 -943.03 -23.50 1.01) ; 6 + ( -337.54 -943.16 -23.50 1.01) ; 7 + ( + ( -339.31 -945.19 -23.75 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-1 + ( -341.21 -946.71 -23.75 0.63) ; 2 + ( -343.36 -947.46 -23.75 0.63) ; 3 + ( -343.62 -947.46 -23.75 0.63) ; 4 + ( -346.40 -947.97 -23.75 0.63) ; 5 + Normal + | + ( -340.32 -943.03 -23.50 1.01) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2 + ( -342.98 -942.65 -23.50 1.01) ; 2 + ( -343.36 -942.65 -23.50 1.01) ; 3 + ( -343.62 -942.65 -23.50 1.01) ; 4 + ( + ( -345.64 -944.05 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-1 + ( -347.16 -945.19 -23.50 0.63) ; 2 + ( -350.32 -945.57 -23.50 0.63) ; 3 + ( 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R-1-1-2-1-1-2-2-2-2-2-2-2-2-2 + ( -356.27 -943.79 -23.50 1.01) ; 2 + ( -357.29 -943.92 -23.50 1.01) ; 3 + ( + ( -357.03 -946.58 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-1 + ( -357.03 -946.71 -23.50 0.63) ; 2 + ( -358.68 -948.73 -23.50 0.63) ; 3 + ( -358.81 -948.73 -23.50 0.63) ; 4 + ( -360.07 -949.62 -23.50 0.63) ; 5 + ( -360.20 -949.74 -23.50 0.63) ; 6 + ( -362.22 -951.01 -23.50 0.63) ; 7 + ( -362.35 -951.01 -23.50 0.63) ; 8 + ( -364.25 -952.02 -23.50 0.63) ; 9 + ( -368.05 -953.67 -23.50 0.63) ; 10 + ( -368.43 -953.79 -23.50 0.63) ; 11 + ( -368.55 -953.92 -23.50 0.63) ; 12 + ( -370.20 -954.93 -23.50 0.63) ; 13 + ( -370.58 -955.06 -23.50 0.63) ; 14 + ( -371.72 -956.58 -23.50 0.63) ; 15 + ( -371.97 -956.83 -23.50 0.63) ; 16 + ( -372.60 -958.10 -23.50 0.63) ; 17 + ( -372.60 -959.49 -23.50 0.63) ; 18 + ( -372.60 -959.87 -23.50 0.63) ; 19 + ( -372.98 -960.63 -23.50 0.63) ; 20 + Normal + | + ( -358.30 -944.17 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2 + ( + ( -360.20 -942.40 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2-1 + ( -360.32 -942.40 -23.50 0.63) ; 2 + ( -362.73 -941.64 -23.50 0.63) ; 3 + ( -362.98 -941.64 -23.50 0.63) ; 4 + ( -366.53 -941.90 -23.50 0.63) ; 5 + ( -366.78 -941.90 -23.50 0.63) ; 6 + ( -368.30 -941.14 -23.50 0.63) ; 7 + ( -368.68 -941.14 -23.50 0.63) ; 8 + ( -368.81 -941.01 -23.50 0.63) ; 9 + ( -369.69 -940.25 -23.50 0.63) ; 10 + ( + ( -371.59 -939.74 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2-1-1 + ( -371.72 -939.74 -23.50 0.63) ; 2 + ( -372.35 -939.36 -23.50 0.63) ; 3 + Normal + | + ( -369.31 -937.72 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2-1-2 + ( -369.31 -937.59 -23.50 0.63) ; 2 + ( -369.69 -936.07 -23.50 0.63) ; 3 + ( -369.82 -935.57 -23.50 0.63) ; 4 + Normal + ) ; End of split + | + ( -360.20 -944.81 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2-2 + ( + ( -362.10 -944.05 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2-2-1 + ( -362.22 -944.05 -23.50 0.63) ; 2 + ( -364.50 -943.92 -23.50 0.63) ; 3 + ( 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-23.50 0.63) ; 4 + ( -368.55 -946.83 -23.50 0.63) ; 5 + ( -369.18 -946.96 -23.50 0.63) ; 6 + ( -369.44 -947.09 -23.50 0.63) ; 7 + ( -369.69 -947.09 -23.50 0.63) ; 8 + ( -370.20 -947.34 -23.50 0.63) ; 9 + ( -370.32 -947.34 -23.50 0.63) ; 10 + ( -371.21 -947.84 -23.50 0.63) ; 11 + ( -371.34 -947.84 -23.50 0.63) ; 12 + ( -371.97 -948.22 -23.50 0.63) ; 13 + ( -372.10 -948.35 -23.50 0.63) ; 14 + Normal + | + ( -366.15 -949.36 -23.50 0.63) ; 1, R-1-1-2-1-1-2-2-2-2-2-2-2-2-2-2-2-2-2 + ( -366.27 -949.36 -23.50 0.63) ; 2 + ( -367.54 -950.63 -23.50 0.63) ; 3 + ( -367.67 -950.76 -23.50 0.63) ; 4 + ( -368.55 -950.88 -23.50 0.63) ; 5 + ( -369.31 -950.88 -23.50 0.63) ; 6 + ( -369.94 -951.52 -23.50 0.63) ; 7 + ( -370.07 -951.64 -23.50 0.63) ; 8 + ( -370.96 -952.28 -23.50 0.63) ; 9 + ( -371.21 -952.53 -23.50 0.63) ; 10 + ( -371.46 -952.78 -23.50 0.63) ; 11 + ( -371.72 -953.03 -23.50 0.63) ; 12 + ( -372.48 -953.92 -23.50 0.63) ; 13 + ( -372.60 -954.05 -23.50 0.63) ; 14 + ( -372.73 -954.17 -23.50 0.63) ; 15 + ( -372.98 -954.30 -23.50 0.63) ; 16 + ( -373.24 -954.43 -23.50 0.63) ; 17 + ( -373.49 -954.81 -23.50 0.63) ; 18 + ( -373.62 -954.81 -23.50 0.63) ; 19 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -284.35 -935.16 -24.00 1.01) ; 1, R-1-1-2-1-2 + ( -284.61 -935.16 -24.00 1.01) ; 2 + ( -286.63 -938.96 -24.75 1.01) ; 3 + ( -286.63 -939.47 -24.75 1.01) ; 4 + ( -288.91 -943.01 -25.75 1.01) ; 5 + ( -290.94 -947.82 -26.25 1.01) ; 6 + ( -291.95 -951.62 -27.00 1.01) ; 7 + ( -293.21 -953.90 -27.75 1.01) ; 8 + ( -292.71 -953.90 -27.75 1.01) ; 9 + ( + ( -297.77 -955.16 -28.00 1.01) ; 1, R-1-1-2-1-2-1 + ( -298.28 -955.16 -28.00 1.01) ; 2 + ( -306.88 -955.67 -28.00 1.01) ; 3 + ( -307.14 -955.67 -28.00 1.01) ; 4 + ( -310.43 -953.64 -28.00 1.01) ; 5 + ( -310.68 -953.64 -28.00 1.01) ; 6 + Normal + | + ( -294.23 -958.71 -28.75 1.01) ; 1, R-1-1-2-1-2-2 + ( + ( -299.04 -962.25 -28.75 1.01) ; 1, R-1-1-2-1-2-2-1 + Normal + | + ( -291.95 -961.75 -28.50 1.01) ; 1, R-1-1-2-1-2-2-2 + ( -292.20 -961.75 -28.50 1.01) ; 2 + ( + ( -288.15 -961.49 -28.50 1.01) ; 1, R-1-1-2-1-2-2-2-1 + Normal + | + ( -290.68 -965.04 -28.50 1.01) ; 1, R-1-1-2-1-2-2-2-2 + ( -290.68 -965.29 -28.50 1.01) ; 2 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -239.31 -919.73 -25.25 2.15) ; 1, R-1-1-2-2 + ( -240.19 -917.33 -25.25 2.15) ; 2 + ( -241.20 -915.93 -24.00 2.15) ; 3 + ( -242.73 -913.66 -24.00 2.15) ; 4 + ( + ( -240.70 -912.26 -24.00 1.52) ; 1, R-1-1-2-2-1 + ( -237.53 -911.13 -24.00 1.52) ; 2 + ( -233.92 -909.95 -24.00 1.52) ; 3 + ( + ( -231.33 -910.75 -24.00 0.76) ; 1, R-1-1-2-2-1-1 + ( -229.69 -911.88 -24.00 0.76) ; 2 + ( -229.56 -911.88 -24.00 0.76) ; 3 + ( -228.17 -916.44 -23.50 0.76) ; 4 + ( -228.17 -917.45 -23.50 0.76) ; 5 + ( + ( -226.02 -919.35 -24.00 0.76) ; 1, R-1-1-2-2-1-1-1 + ( -225.51 -919.35 -24.00 0.76) ; 2 + Normal + | + ( -228.29 -918.59 -23.50 0.76) ; 1, R-1-1-2-2-1-1-2 + ( + ( -227.66 -921.38 -23.50 0.76) ; 1, R-1-1-2-2-1-1-2-1 + ( -227.53 -921.51 -23.50 0.76) ; 2 + ( -227.28 -922.39 -23.50 0.76) ; 3 + ( -227.15 -922.64 -23.50 0.76) ; 4 + ( -227.15 -922.64 -23.50 0.76) ; 5 + Normal + | + ( -230.19 -921.00 -23.50 0.76) ; 1, R-1-1-2-2-1-1-2-2 + ( -230.95 -921.51 -23.50 0.76) ; 2 + ( -230.95 -921.63 -23.50 0.76) ; 3 + ( -230.95 -922.52 -23.50 0.76) ; 4 + ( -230.45 -923.28 -23.50 0.76) ; 5 + Normal + ) ; End of split + ) ; End of split + | + ( -232.48 -908.97 -23.50 1.39) ; 1, R-1-1-2-2-1-2 + ( -231.08 -906.95 -23.50 1.14) ; 2 + ( -229.69 -905.05 -23.50 1.01) ; 3 + ( -228.42 -903.78 -23.50 1.01) ; 4 + ( -226.14 -901.63 -23.50 1.01) ; 5 + ( -224.50 -901.00 -23.50 1.01) ; 6 + ( -224.37 -901.00 -23.50 1.01) ; 7 + ( -222.22 -897.33 -24.25 0.76) ; 8 + ( + ( -222.15 -895.09 -23.75 0.96) ; 1, R-1-1-2-2-1-2-1 + ( -221.84 -892.06 -23.75 0.96) ; 2 + ( -221.52 -890.63 -23.75 0.96) ; 3 + ( -221.52 -890.15 -23.75 0.96) ; 4 + ( -221.20 -889.67 -23.75 0.96) ; 5 + ( -221.04 -889.36 -23.75 0.96) ; 6 + ( -220.88 -888.88 -23.75 0.96) ; 7 + ( -220.56 -888.56 -23.75 0.96) ; 8 + ( -220.24 -888.08 -23.75 0.96) ; 9 + ( -220.08 -887.60 -23.75 0.96) ; 10 + ( -219.45 -886.97 -23.75 0.96) ; 11 + ( -219.13 -886.49 -23.75 0.96) ; 12 + ( -218.01 -885.38 -23.75 0.96) ; 13 + ( -217.70 -885.06 -23.75 0.96) ; 14 + ( -217.38 -884.90 -23.75 0.96) ; 15 + ( -217.06 -884.74 -23.75 0.96) ; 16 + ( -216.74 -884.74 -23.75 0.96) ; 17 + Normal + | + ( -219.29 -895.72 -23.75 0.96) ; 1, R-1-1-2-2-1-2-2 + ( + ( -215.47 -895.25 -23.75 0.96) ; 1, R-1-1-2-2-1-2-2-1 + ( -215.63 -895.25 -23.75 0.96) ; 2 + ( + ( -212.28 -894.77 -23.75 0.96) ; 1, R-1-1-2-2-1-2-2-1-1 + ( -210.37 -893.18 -23.75 0.96) ; 2 + ( -210.05 -893.02 -23.75 0.96) ; 3 + Normal + | + ( -214.83 -892.22 -23.75 0.96) ; 1, R-1-1-2-2-1-2-2-1-2 + ( -214.67 -891.90 -23.75 0.96) ; 2 + ( -213.56 -889.36 -23.75 0.96) ; 3 + Normal + ) ; End of split + | + ( -218.81 -893.18 -23.75 0.96) ; 1, R-1-1-2-2-1-2-2-2 + ( -218.65 -892.86 -23.75 0.96) ; 2 + ( -217.38 -889.83 -23.75 0.96) ; 3 + ( -217.22 -889.67 -23.75 0.96) ; 4 + ( -214.99 -888.56 -23.75 0.96) ; 5 + ( -215.15 -888.40 -23.75 0.96) ; 6 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -244.12 -912.39 -24.00 2.5) ; 1, R-1-1-2-2-2 + ( + ( -242.22 -909.73 -24.00 1.90) ; 1, R-1-1-2-2-2-1 + ( -241.58 -906.57 -24.00 1.77) ; 2 + ( -241.33 -904.92 -24.00 1.77) ; 3 + ( + ( -242.47 -903.02 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1 + ( -243.74 -901.38 -24.00 0.89) ; 2 + ( -245.00 -898.72 -24.00 0.89) ; 3 + ( -246.65 -895.81 -24.00 0.89) ; 4 + ( -247.28 -893.02 -24.00 0.89) ; 5 + ( -248.04 -890.75 -24.00 0.89) ; 6 + ( -248.80 -887.83 -24.00 0.89) ; 7 + ( + ( -248.17 -881.13 -23.75 0.89) ; 1, R-1-1-2-2-2-1-1-1 + ( + ( -245.00 -879.61 -23.75 0.89) ; 1, R-1-1-2-2-2-1-1-1-1 + ( -243.99 -878.59 -23.75 0.89) ; 2 + ( -241.96 -877.33 -23.75 0.89) ; 3 + ( -241.71 -877.33 -23.75 0.89) ; 4 + ( -239.31 -876.19 -23.75 0.89) ; 5 + ( -238.80 -874.92 -23.75 0.89) ; 6 + ( -238.80 -874.54 -23.75 0.89) ; 7 + ( -238.80 -872.39 -23.75 0.89) ; 8 + ( -238.93 -872.01 -23.75 0.89) ; 9 + ( -240.19 -870.11 -23.75 0.89) ; 10 + ( -240.19 -869.73 -23.75 0.89) ; 11 + ( -240.45 -868.47 -23.75 0.89) ; 12 + Normal + | + ( -247.66 -876.19 -23.75 0.89) ; 1, R-1-1-2-2-2-1-1-1-2 + ( -246.52 -873.28 -23.75 0.89) ; 2 + ( + ( -245.00 -869.73 -23.75 0.89) ; 1, R-1-1-2-2-2-1-1-1-2-1 + ( -243.61 -867.33 -23.75 0.89) ; 2 + ( -242.60 -866.06 -23.75 0.89) ; 3 + Normal + | + ( -248.17 -871.38 -23.75 0.89) ; 1, R-1-1-2-2-2-1-1-1-2-2 + ( -248.29 -871.38 -23.75 0.89) ; 2 + ( -249.18 -869.23 -23.75 0.89) ; 3 + ( -249.31 -868.97 -23.75 0.89) ; 4 + ( -250.19 -867.20 -23.75 0.89) ; 5 + ( -250.32 -866.95 -23.75 0.89) ; 6 + ( -251.33 -865.68 -23.75 0.89) ; 7 + Normal + ) ; End of split + ) ; End of split + | + ( -251.21 -885.94 -24.00 1.14) ; 1, R-1-1-2-2-2-1-1-2 + ( -253.36 -884.16 -24.00 1.14) ; 2 + ( -253.99 -883.53 -24.00 1.14) ; 3 + ( + ( -254.75 -882.39 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-1 + ( + ( -254.62 -880.24 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-1-1 + ( -254.62 -876.19 -24.00 0.89) ; 2 + ( -254.62 -875.68 -24.00 0.89) ; 3 + ( + ( -255.89 -874.04 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-1-1-1 + ( -257.03 -871.88 -24.00 0.89) ; 2 + ( -256.90 -870.11 -24.00 0.89) ; 3 + ( -256.90 -869.86 -24.00 0.89) ; 4 + ( -255.89 -867.58 -24.00 0.89) ; 5 + Normal + | + ( -253.61 -872.77 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-1-1-2 + ( -253.61 -872.52 -24.00 0.89) ; 2 + ( -253.36 -869.86 -24.00 0.89) ; 3 + ( -253.23 -869.86 -24.00 0.89) ; 4 + Normal + ) ; End of split + | + ( -256.27 -881.63 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-1-2 + ( -257.53 -880.62 -24.00 0.89) ; 2 + ( -258.29 -879.86 -24.00 0.89) ; 3 + ( -259.43 -877.96 -24.00 0.89) ; 4 + ( -259.56 -876.44 -24.00 0.89) ; 5 + ( -259.56 -874.67 -24.00 0.89) ; 6 + ( -259.56 -874.29 -24.00 0.89) ; 7 + ( -260.95 -871.13 -24.00 0.89) ; 8 + ( -261.96 -869.61 -24.00 0.89) ; 9 + Normal + ) ; End of split + | + ( -256.39 -883.40 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-2 + ( -256.65 -883.40 -24.00 0.89) ; 2 + ( -258.29 -882.64 -24.00 0.89) ; 3 + ( -258.55 -882.64 -24.00 0.89) ; 4 + ( -260.32 -881.76 -24.00 0.89) ; 5 + ( -261.96 -880.24 -24.00 0.89) ; 6 + ( -263.86 -878.59 -24.00 0.89) ; 7 + ( -264.88 -877.45 -24.00 0.89) ; 8 + ( + ( -265.64 -875.68 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-2-1 + ( -267.03 -873.40 -24.00 0.89) ; 2 + ( -268.80 -870.87 -24.00 0.89) ; 3 + ( -268.93 -870.75 -24.00 0.89) ; 4 + ( -270.45 -869.61 -24.00 0.89) ; 5 + Normal + | + ( -266.77 -877.20 -24.00 0.89) ; 1, R-1-1-2-2-2-1-1-2-2-2 + ( -267.53 -876.69 -24.00 0.89) ; 2 + ( -269.18 -875.94 -24.00 0.89) ; 3 + ( -269.31 -875.94 -24.00 0.89) ; 4 + ( -271.08 -874.80 -24.00 0.89) ; 5 + ( -271.33 -874.67 -24.00 0.89) ; 6 + ( -272.47 -873.78 -24.00 0.89) ; 7 + ( -272.72 -873.40 -24.00 0.89) ; 8 + ( -273.86 -872.26 -24.00 0.89) ; 9 + ( -273.99 -872.14 -24.00 0.89) ; 10 + ( -274.75 -871.13 -24.00 0.89) ; 11 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -240.19 -904.04 -24.00 2.15) ; 1, R-1-1-2-2-2-1-2 + ( + ( -237.28 -903.68 -23.50 1.43) ; 1, R-1-1-2-2-2-1-2-1 + ( -237.44 -903.68 -23.50 1.43) ; 2 + ( -234.57 -903.21 -23.50 1.27) ; 3 + ( -234.57 -903.05 -23.50 1.27) ; 4 + ( -232.34 -903.05 -23.50 1.11) ; 5 + ( + ( -228.52 -903.37 -22.50 0.96) ; 1, R-1-1-2-2-2-1-2-1-1 + ( -228.68 -903.68 -22.50 0.96) ; 2 + ( + ( -222.63 -907.19 -22.00 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-1 + ( -219.77 -907.82 -21.75 0.96) ; 2 + ( -217.06 -908.14 -22.00 0.96) ; 3 + ( -214.51 -908.94 -22.00 0.96) ; 4 + ( -214.51 -908.78 -22.00 0.96) ; 5 + ( -212.92 -909.42 -22.50 0.96) ; 6 + ( -213.08 -909.42 -22.50 0.96) ; 7 + ( + ( -210.37 -911.01 -23.25 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-1-1 + ( -210.05 -910.85 -23.25 0.96) ; 2 + ( -207.83 -911.17 -23.25 0.96) ; 3 + ( -207.51 -911.01 -23.25 0.96) ; 4 + ( -204.16 -909.89 -23.25 0.96) ; 5 + ( -203.85 -909.73 -23.25 0.96) ; 6 + Normal + | + ( -210.69 -906.87 -22.50 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-1-2 + ( -210.37 -906.55 -22.50 0.96) ; 2 + ( -207.99 -904.80 -22.50 0.96) ; 3 + ( -207.67 -904.64 -22.50 0.96) ; 4 + Normal + ) ; End of split + | + ( -226.77 -902.25 -22.25 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-2 + ( -226.45 -901.93 -22.25 0.96) ; 2 + ( -224.22 -901.30 -22.25 0.96) ; 3 + ( -224.06 -901.14 -22.25 0.96) ; 4 + ( -221.20 -900.02 -22.25 0.96) ; 5 + ( -221.20 -899.86 -22.25 0.96) ; 6 + ( + ( -219.29 -898.43 -22.75 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-2-1 + ( -219.29 -898.27 -22.75 0.96) ; 2 + ( + ( -216.42 -898.75 -22.50 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-2-1-1 + ( -216.26 -898.59 -22.50 0.96) ; 2 + ( -212.28 -899.07 -22.50 0.96) ; 3 + ( -212.44 -899.07 -22.50 0.96) ; 4 + Normal + ) ; End of split + | + ( -219.29 -901.77 -21.75 0.96) ; 1, R-1-1-2-2-2-1-2-1-1-2-2 + ( -219.29 -901.93 -21.75 0.96) ; 2 + Normal + ) ; End of split + ) ; End of split + | + ( -232.02 -900.66 -23.75 0.96) ; 1, R-1-1-2-2-2-1-2-1-2 + ( -231.87 -900.34 -23.75 0.96) ; 2 + ( -230.91 -897.63 -23.75 0.96) ; 3 + ( -230.75 -897.32 -23.75 0.96) ; 4 + ( -230.11 -895.57 -23.75 0.96) ; 5 + ( -229.95 -895.57 -23.75 0.96) ; 6 + Normal + ) ; End of split + | + ( -239.31 -901.51 -24.00 1.01) ; 1, R-1-1-2-2-2-1-2-2 + ( -238.29 -897.96 -24.00 1.01) ; 2 + ( -236.39 -892.77 -23.50 1.01) ; 3 + ( -234.50 -888.47 -23.50 1.01) ; 4 + ( -232.22 -886.19 -23.50 1.01) ; 5 + ( + ( -229.69 -885.56 -23.50 1.01) ; 1, R-1-1-2-2-2-1-2-2-1 + ( -229.43 -885.30 -23.50 1.01) ; 2 + ( -227.03 -884.04 -23.50 1.01) ; 3 + ( -227.03 -883.91 -23.50 1.01) ; 4 + ( + ( -223.36 -883.40 -23.50 0.76) ; 1, R-1-1-2-2-2-1-2-2-1-1 + ( -222.15 -882.51 -24.75 0.96) ; 2 + ( -221.99 -882.19 -24.75 0.96) ; 3 + ( -219.93 -879.17 -24.75 0.96) ; 4 + ( -219.77 -879.17 -24.75 0.96) ; 5 + Normal + | + ( -225.50 -881.56 -24.50 0.96) ; 1, R-1-1-2-2-2-1-2-2-1-2 + ( -225.50 -881.24 -24.50 0.96) ; 2 + ( -223.43 -879.17 -24.50 0.96) ; 3 + ( -221.68 -877.26 -25.50 0.96) ; 4 + ( -221.68 -877.42 -25.50 0.96) ; 5 + ( -218.81 -874.07 -24.50 0.96) ; 6 + Normal + ) ; End of split + | + ( -232.09 -883.62 -23.50 0.76) ; 1, R-1-1-2-2-2-1-2-2-2 + ( -231.45 -881.47 -23.50 0.76) ; 2 + ( + ( -229.68 -877.55 -23.50 0.76) ; 1, R-1-1-2-2-2-1-2-2-2-1 + ( -229.30 -875.27 -23.50 0.76) ; 2 + ( -229.30 -874.76 -23.50 0.76) ; 3 + ( -229.18 -872.74 -23.50 0.76) ; 4 + ( -229.05 -871.98 -23.50 0.76) ; 5 + ( -228.54 -870.84 -23.50 0.76) ; 6 + Normal + | + ( -232.34 -878.43 -23.50 0.76) ; 1, R-1-1-2-2-2-1-2-2-2-2 + ( -232.47 -876.54 -23.50 0.76) ; 2 + ( -232.47 -876.28 -23.50 0.76) ; 3 + ( -233.23 -874.13 -23.50 0.76) ; 4 + ( + ( -235.00 -872.49 -23.50 0.76) ; 1, R-1-1-2-2-2-1-2-2-2-2-1 + ( -236.01 -870.21 -23.50 0.76) ; 2 + ( -236.01 -870.08 -23.50 0.76) ; 3 + ( -236.52 -868.94 -23.50 0.76) ; 4 + ( -237.15 -867.80 -23.50 0.76) ; 5 + Normal + | + ( -232.59 -871.98 -23.50 0.76) ; 1, R-1-1-2-2-2-1-2-2-2-2-2 + ( -232.34 -869.83 -23.50 0.76) ; 2 + ( 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2 + ( -279.17 -880.96 -24.00 1.01) ; 3 + ( -279.81 -879.57 -24.00 1.01) ; 4 + ( -280.95 -878.05 -24.00 1.01) ; 5 + ( + ( -278.67 -874.50 -24.00 1.01) ; 1, R-1-1-2-2-2-2-1-1-1-1 + ( -277.40 -872.35 -24.00 0.76) ; 2 + ( -277.40 -870.83 -24.00 0.76) ; 3 + ( -277.40 -870.07 -24.00 0.76) ; 4 + ( + ( -277.53 -867.79 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-1-1 + ( -277.65 -867.67 -24.00 0.76) ; 2 + ( -278.54 -864.88 -24.00 0.76) ; 3 + ( -278.67 -862.48 -24.00 0.76) ; 4 + Normal + | + ( -276.14 -865.77 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-1-2 + ( -274.24 -864.00 -24.00 0.76) ; 2 + ( -274.11 -864.00 -24.00 0.76) ; 3 + ( -272.46 -862.86 -24.00 0.76) ; 4 + ( -271.96 -862.48 -24.00 0.76) ; 5 + ( -270.95 -861.72 -24.00 0.76) ; 6 + ( -270.69 -861.47 -24.00 0.76) ; 7 + ( -270.44 -859.95 -24.00 0.76) ; 8 + Normal + ) ; End of split + | + ( -282.21 -877.04 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2 + ( + ( -281.83 -872.48 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2-1 + ( -281.45 -870.20 -24.00 0.76) ; 2 + ( -280.95 -866.78 -24.00 0.76) ; 3 + ( + ( -280.44 -864.50 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2-1-1 + ( -278.03 -862.73 -24.00 0.76) ; 2 + ( -276.39 -861.34 -24.00 0.76) ; 3 + Normal + | + ( -281.96 -864.63 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2-1-2 + ( -282.72 -862.98 -24.00 0.76) ; 2 + ( -283.35 -861.97 -24.00 0.76) ; 3 + Normal + ) ; End of split + | + ( -284.62 -875.90 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2-2 + ( -285.63 -875.01 -24.00 0.76) ; 2 + ( -286.64 -873.24 -24.00 0.76) ; 3 + ( -286.77 -873.11 -24.00 0.76) ; 4 + ( -287.02 -869.06 -24.00 0.76) ; 5 + ( -287.15 -865.52 -24.00 0.76) ; 6 + ( + ( -288.03 -864.50 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2-2-1 + ( -288.29 -864.38 -24.00 0.76) ; 2 + ( -289.43 -862.60 -24.00 0.76) ; 3 + ( -289.55 -862.23 -24.00 0.76) ; 4 + ( -290.44 -861.34 -24.00 0.76) ; 5 + Normal + | + ( -286.14 -863.36 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-1-2-2-2 + ( -284.87 -861.59 -24.00 0.76) ; 2 + ( -284.62 -861.34 -24.00 0.76) ; 3 + ( -283.60 -860.71 -24.00 0.76) ; 4 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -281.07 -883.87 -24.00 1.01) ; 1, R-1-1-2-2-2-2-1-1-2 + ( -283.35 -881.97 -24.00 1.01) ; 2 + ( -284.62 -880.83 -24.00 1.01) ; 3 + ( -286.14 -880.07 -24.25 1.01) ; 4 + ( + ( -288.16 -880.71 -24.25 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-1 + ( -292.09 -879.95 -24.25 0.76) ; 2 + ( -294.74 -879.31 -24.25 0.76) ; 3 + ( -297.53 -878.93 -24.25 0.76) ; 4 + ( + ( -300.95 -878.55 -24.25 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-1-1 + ( -301.07 -878.55 -24.25 0.76) ; 2 + ( -302.84 -878.43 -24.25 0.76) ; 3 + ( -303.60 -878.43 -24.25 0.76) ; 4 + ( -304.62 -878.43 -24.25 0.76) ; 5 + ( -304.87 -878.43 -24.25 0.76) ; 6 + ( -306.01 -877.54 -24.25 0.76) ; 7 + Normal + | + ( -298.92 -877.54 -24.25 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-1-2 + ( -300.57 -876.40 -24.25 0.76) ; 2 + ( -300.69 -876.28 -24.25 0.76) ; 3 + ( -301.96 -875.14 -24.25 0.76) ; 4 + ( -302.09 -875.01 -24.25 0.76) ; 5 + ( -303.48 -874.00 -24.25 0.76) ; 6 + ( -304.62 -873.36 -24.25 0.76) ; 7 + ( -306.52 -873.36 -24.25 0.76) ; 8 + ( -307.02 -873.49 -24.25 0.76) ; 9 + ( -307.15 -873.49 -24.25 0.76) ; 10 + ( -307.53 -873.49 -24.25 0.76) ; 11 + ( -307.65 -873.49 -24.25 0.76) ; 12 + Normal + ) ; End of split + | + ( -288.16 -878.05 -24.25 1.14) ; 1, R-1-1-2-2-2-2-1-1-2-2 + ( -291.96 -875.52 -24.25 1.14) ; 2 + ( -293.60 -873.24 -24.25 1.14) ; 3 + ( -296.01 -871.34 -24.25 1.14) ; 4 + ( -296.14 -871.34 -24.25 1.14) ; 5 + ( -296.52 -871.34 -24.25 1.14) ; 6 + ( + ( -298.92 -871.21 -24.25 1.14) ; 1, R-1-1-2-2-2-2-1-1-2-2-1 + ( -301.45 -870.33 -24.25 1.14) ; 2 + ( -301.71 -870.07 -24.25 1.14) ; 3 + ( -303.60 -869.06 -24.25 1.14) ; 4 + ( -306.14 -868.05 -24.25 1.14) ; 5 + ( -309.43 -866.28 -24.25 1.01) ; 6 + ( -311.96 -865.14 -24.25 1.01) ; 7 + ( -312.09 -865.14 -24.25 1.01) ; 8 + ( -315.38 -864.38 -24.25 1.01) ; 9 + ( -315.76 -864.38 -24.25 1.01) ; 10 + ( -318.29 -864.50 -24.25 1.01) ; 11 + ( -318.41 -864.50 -24.25 1.01) ; 12 + ( -319.81 -864.63 -24.25 1.01) ; 13 + ( + ( -322.72 -864.88 -24.25 1.01) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1 + ( -322.97 -864.88 -24.25 1.01) ; 2 + ( -325.00 -864.88 -24.25 1.01) ; 3 + ( -325.38 -864.88 -24.25 1.01) ; 4 + ( -326.90 -864.50 -24.25 1.01) ; 5 + ( -327.28 -864.25 -24.25 1.01) ; 6 + ( -329.68 -863.24 -24.25 1.01) ; 7 + ( -330.06 -863.24 -24.25 1.01) ; 8 + ( -332.09 -862.10 -24.25 1.01) ; 9 + ( -332.21 -862.10 -24.25 1.01) ; 10 + ( + ( -336.39 -863.62 -24.25 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1-1 + ( -336.64 -863.62 -24.25 0.76) ; 2 + ( -339.45 -864.32 -24.25 0.76) ; 3 + ( -342.84 -866.28 -24.50 0.76) ; 4 + ( -345.25 -866.28 -24.50 0.76) ; 5 + ( -345.50 -866.02 -24.50 0.76) ; 6 + ( -345.88 -865.90 -24.50 0.76) ; 7 + ( -346.64 -865.26 -24.50 0.76) ; 8 + ( -347.02 -865.14 -24.50 0.76) ; 9 + ( -348.29 -864.63 -24.50 0.76) ; 10 + ( -348.79 -864.63 -24.50 0.76) ; 11 + ( -350.57 -864.63 -24.50 0.76) ; 12 + ( -353.73 -861.97 -24.50 0.76) ; 13 + ( -353.98 -861.85 -24.50 0.76) ; 14 + ( -354.87 -860.83 -24.50 0.76) ; 15 + ( -355.25 -860.58 -24.50 0.76) ; 16 + ( -355.63 -859.95 -24.50 0.76) ; 17 + ( -356.14 -859.57 -24.50 0.76) ; 18 + ( -357.02 -858.93 -24.50 0.76) ; 19 + ( -357.15 -858.81 -24.50 0.76) ; 20 + ( -357.28 -858.81 -24.50 0.76) ; 21 + ( -357.53 -858.68 -24.50 0.76) ; 22 + ( -357.65 -858.68 -24.50 0.76) ; 23 + Normal + | + ( -333.35 -861.85 -25.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1-2 + ( + ( -335.63 -859.82 -25.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1-2-1 + ( -336.01 -859.57 -25.00 0.76) ; 2 + ( + ( -333.48 -857.41 -25.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1-2-1-1 + ( -332.21 -856.66 -25.00 0.76) ; 2 + ( -331.45 -856.78 -25.00 0.76) ; 3 + ( -330.57 -856.78 -25.00 0.76) ; 4 + Normal + | + ( -337.02 -857.79 -25.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1-2-1-2 + ( -340.44 -856.28 -25.00 0.76) ; 2 + ( -340.57 -856.28 -25.00 0.76) ; 3 + ( -343.22 -855.90 -25.00 0.76) ; 4 + ( -345.00 -855.01 -25.00 0.76) ; 5 + ( -345.25 -855.01 -25.00 0.76) ; 6 + ( -347.28 -855.01 -25.00 0.76) ; 7 + ( -348.03 -854.63 -25.00 0.76) ; 8 + ( -349.05 -854.38 -25.00 0.76) ; 9 + ( -349.30 -854.38 -25.00 0.76) ; 10 + ( -350.19 -854.38 -25.00 0.76) ; 11 + Normal + ) ; End of split + | + ( -336.64 -862.48 -25.00 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-1-2-2 + ( -336.64 -862.35 -25.00 0.76) ; 2 + ( -338.54 -860.96 -25.00 0.76) ; 3 + ( -338.79 -860.83 -25.00 0.76) ; 4 + ( -340.31 -859.57 -25.00 0.76) ; 5 + ( -340.82 -859.31 -25.00 0.76) ; 6 + ( -340.95 -859.19 -25.00 0.76) ; 7 + ( -341.96 -858.93 -25.00 0.76) ; 8 + ( -342.72 -858.93 -25.00 0.76) ; 9 + ( -345.25 -858.17 -25.00 0.76) ; 10 + ( -346.14 -858.17 -25.00 0.76) ; 11 + ( -346.90 -857.79 -25.00 0.76) ; 12 + ( -347.28 -857.79 -25.00 0.76) ; 13 + ( -347.65 -857.79 -25.00 0.76) ; 14 + ( -349.05 -857.79 -25.00 0.76) ; 15 + ( -349.55 -857.79 -25.00 0.76) ; 16 + ( -350.57 -857.67 -25.00 0.76) ; 17 + ( -351.07 -857.67 -25.00 0.76) ; 18 + ( -351.20 -857.54 -25.00 0.76) ; 19 + ( -351.83 -857.54 -25.00 0.76) ; 20 + ( -351.96 -857.54 -25.00 0.76) ; 21 + ( -352.34 -857.54 -25.00 0.76) ; 22 + ( -352.72 -857.54 -25.00 0.76) ; 23 + ( -353.48 -857.54 -25.00 0.76) ; 24 + ( -353.60 -857.54 -25.00 0.76) ; 25 + Normal + ) ; End of split + ) ; End of split + | + ( -321.96 -860.96 -24.25 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-1-2 + ( -323.73 -859.19 -24.25 0.76) ; 2 + ( -324.87 -857.04 -24.25 0.76) ; 3 + ( -325.00 -856.78 -24.25 0.76) ; 4 + ( -326.14 -854.88 -24.25 0.76) ; 5 + ( -329.93 -853.49 -24.25 0.76) ; 6 + ( -332.84 -852.86 -24.25 0.76) ; 7 + ( -332.97 -852.86 -24.25 0.76) ; 8 + ( -334.87 -852.60 -24.25 0.76) ; 9 + ( -335.25 -852.60 -24.25 0.76) ; 10 + ( -336.64 -852.60 -24.25 0.76) ; 11 + ( -336.77 -852.60 -24.25 0.76) ; 12 + ( -337.40 -852.48 -24.25 0.76) ; 13 + ( -337.53 -852.35 -24.25 0.76) ; 14 + ( -337.91 -852.35 -24.25 0.76) ; 15 + Normal + ) ; End of split + | + ( -297.91 -868.43 -24.25 1.39) ; 1, R-1-1-2-2-2-2-1-1-2-2-2 + ( -300.69 -865.26 -24.25 1.39) ; 2 + ( -303.10 -862.35 -24.25 1.39) ; 3 + ( -303.73 -861.59 -24.25 1.39) ; 4 + ( + ( -303.35 -859.44 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-1 + ( -303.10 -855.52 -24.25 0.89) ; 2 + ( -302.46 -853.74 -24.25 0.89) ; 3 + ( + ( -304.13 -852.04 -25.50 0.64) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-1-1 + ( -304.29 -851.88 -25.50 0.64) ; 2 + ( -307.63 -851.08 -25.50 0.64) ; 3 + ( -307.95 -851.40 -25.50 0.64) ; 4 + ( -312.25 -851.24 -25.50 0.64) ; 5 + Normal + | + ( -300.41 -851.38 -24.25 0.76) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-1-2 + ( -300.29 -851.25 -24.25 0.76) ; 2 + ( -297.76 -848.98 -24.25 0.76) ; 3 + ( -297.63 -848.72 -24.25 0.76) ; 4 + ( -297.38 -846.82 -24.25 0.76) ; 5 + ( -297.38 -846.57 -24.25 0.76) ; 6 + ( -297.25 -842.39 -24.25 0.76) ; 7 + ( -297.25 -842.27 -24.25 0.76) ; 8 + Normal + ) ; End of split + | + ( -306.25 -860.31 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2 + ( -309.16 -858.67 -24.25 0.89) ; 2 + ( -310.43 -858.16 -24.25 0.89) ; 3 + ( -311.19 -857.65 -24.25 0.89) ; 4 + ( + ( -314.73 -858.41 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-1 + ( -315.11 -858.41 -24.25 0.89) ; 2 + ( -317.01 -858.79 -24.25 0.89) ; 3 + ( -317.65 -858.79 -24.25 0.89) ; 4 + ( -318.41 -858.92 -24.25 0.89) ; 5 + ( -319.16 -859.05 -24.25 0.89) ; 6 + ( -319.67 -859.05 -24.25 0.89) ; 7 + Normal + | + ( -311.19 -857.02 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2 + ( + ( -314.35 -856.64 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2-1 + ( -316.00 -855.37 -24.25 0.89) ; 2 + ( -316.38 -855.37 -24.25 0.89) ; 3 + ( + ( -319.92 -854.87 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2-1-1 + ( -320.05 -854.87 -24.25 0.89) ; 2 + ( -321.44 -854.74 -24.25 0.89) ; 3 + ( -321.70 -854.49 -24.25 0.89) ; 4 + ( -322.58 -853.86 -24.25 0.89) ; 5 + ( -322.71 -853.60 -24.25 0.89) ; 6 + ( -322.84 -853.60 -24.25 0.89) ; 7 + ( -323.47 -852.97 -24.25 0.89) ; 8 + ( -323.85 -852.72 -24.25 0.89) ; 9 + ( -324.99 -852.08 -24.25 0.89) ; 10 + ( -325.49 -851.96 -24.25 0.89) ; 11 + ( -325.75 -851.96 -24.25 0.89) ; 12 + ( -326.51 -851.58 -24.25 0.89) ; 13 + ( -327.01 -851.45 -24.25 0.89) ; 14 + ( -327.27 -851.45 -24.25 0.89) ; 15 + ( 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-24.25 0.89) ; 4 + ( -332.71 -843.60 -24.25 0.89) ; 5 + ( -332.96 -842.97 -24.25 0.89) ; 6 + ( -333.09 -841.83 -24.25 0.89) ; 7 + Normal + | + ( -331.57 -848.79 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2-1-2-2 + ( -332.96 -848.79 -24.25 0.89) ; 2 + ( + ( -335.49 -849.30 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2-1-2-2-1 + ( -335.87 -849.30 -24.25 0.89) ; 2 + ( -337.65 -849.30 -24.25 0.89) ; 3 + ( -338.53 -849.30 -24.25 0.89) ; 4 + ( -339.16 -849.43 -24.25 0.89) ; 5 + ( -339.42 -849.55 -24.25 0.89) ; 6 + ( -339.67 -849.55 -24.25 0.89) ; 7 + ( -339.80 -849.68 -24.25 0.89) ; 8 + ( -339.92 -849.68 -24.25 0.89) ; 9 + Normal + | + ( -335.75 -846.39 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2-1-2-2-2 + ( -335.87 -846.26 -24.25 0.89) ; 2 + ( -336.76 -845.37 -24.25 0.89) ; 3 + ( -336.76 -845.12 -24.25 0.89) ; 4 + ( -337.65 -843.73 -24.25 0.89) ; 5 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -312.08 -853.35 -24.25 0.89) ; 1, R-1-1-2-2-2-2-1-1-2-2-2-2-2-2 + ( 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-23.75 0.76) ; 4 + ( -269.68 -908.28 -23.75 0.76) ; 5 + ( -269.68 -908.40 -23.75 0.76) ; 6 + ( -268.29 -908.91 -23.75 0.76) ; 7 + ( + ( -266.89 -910.43 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1 + ( -266.64 -912.58 -23.75 0.76) ; 2 + ( -266.64 -913.09 -23.75 0.76) ; 3 + ( + ( -267.15 -916.00 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1-1 + ( -268.29 -918.28 -23.75 0.76) ; 2 + ( -268.29 -918.66 -23.75 0.76) ; 3 + ( -268.54 -920.55 -23.75 0.76) ; 4 + ( -268.54 -921.06 -23.75 0.76) ; 5 + ( -268.79 -922.45 -23.75 0.76) ; 6 + ( -268.92 -922.58 -23.75 0.76) ; 7 + ( + ( -270.31 -925.24 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1-1-1 + ( -271.32 -926.63 -23.75 0.76) ; 2 + Normal + | + ( -267.91 -924.61 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1-1-2 + ( -267.65 -924.61 -23.75 0.76) ; 2 + ( -266.51 -924.73 -23.75 0.76) ; 3 + ( -266.01 -924.73 -23.75 0.76) ; 4 + ( -265.37 -925.36 -23.75 0.76) ; 5 + ( -265.12 -925.36 -23.75 0.76) ; 6 + Normal + ) ; End of split + | + ( -263.10 -915.87 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1-2 + ( + ( -262.21 -920.43 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1-2-1 + ( -262.21 -922.45 -23.75 0.76) ; 2 + ( -262.46 -922.71 -23.75 0.76) ; 3 + ( -263.10 -923.47 -23.75 0.76) ; 4 + ( -263.22 -923.59 -23.75 0.76) ; 5 + ( -263.35 -923.72 -23.75 0.76) ; 6 + Normal + | + ( -259.93 -917.64 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-1-2-2 + ( -258.79 -918.15 -23.75 0.76) ; 2 + ( -258.41 -918.40 -23.75 0.76) ; 3 + ( -256.01 -920.05 -23.75 0.76) ; 4 + ( -255.75 -920.17 -23.75 0.76) ; 5 + ( -254.49 -920.81 -23.75 0.76) ; 6 + ( -253.60 -920.93 -23.75 0.76) ; 7 + ( -253.10 -921.31 -23.75 0.76) ; 8 + Normal + ) ; End of split + ) ; End of split + | + ( -264.74 -908.78 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2 + ( + ( -261.70 -907.26 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2-1 + ( -261.70 -905.87 -23.75 0.76) ; 2 + Normal + | + ( -259.43 -909.92 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2-2 + ( + ( -259.30 -912.83 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2-2-1 + ( -259.30 -913.09 -23.75 0.76) ; 2 + ( -259.17 -914.98 -23.75 0.76) ; 3 + ( -259.17 -915.49 -23.75 0.76) ; 4 + ( -259.17 -916.38 -23.75 0.76) ; 5 + ( -259.17 -916.50 -23.75 0.76) ; 6 + ( -259.17 -916.88 -23.75 0.76) ; 7 + Normal + | + ( -257.15 -910.05 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2-2-2 + ( -256.89 -910.30 -23.75 0.76) ; 2 + ( -256.51 -910.55 -23.75 0.76) ; 3 + ( + ( -253.35 -913.34 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2-2-2-1 + ( -253.10 -913.72 -23.75 0.76) ; 2 + ( -251.96 -915.49 -23.75 0.76) ; 3 + ( -251.83 -915.62 -23.75 0.76) ; 4 + ( -251.58 -915.87 -23.75 0.76) ; 5 + ( -251.45 -916.12 -23.75 0.76) ; 6 + ( -251.32 -916.38 -23.75 0.76) ; 7 + ( -250.94 -917.39 -23.75 0.76) ; 8 + ( -250.69 -918.02 -23.75 0.76) ; 9 + Normal + | + ( -252.97 -911.06 -23.75 0.76) ; 1, R-1-1-2-2-2-2-1-2-1-1-2-2-2-2 + ( -251.32 -911.95 -23.75 0.76) ; 2 + ( -251.07 -912.45 -23.75 0.76) ; 3 + ( -250.94 -912.58 -23.75 0.76) ; 4 + ( -249.68 -912.83 -23.75 0.76) ; 5 + ( -249.43 -913.34 -23.75 0.76) ; 6 + ( 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7 + ( -273.35 -917.52 -23.75 0.76) ; 8 + ( -273.35 -917.64 -23.75 0.76) ; 9 + ( -273.35 -918.28 -23.75 0.76) ; 10 + ( -273.60 -918.53 -23.75 0.76) ; 11 + ( -273.60 -918.78 -23.75 0.76) ; 12 + ( -273.60 -919.16 -23.75 0.76) ; 13 + Normal + ) ; End of split + ) ; End of split + | + ( -278.41 -905.49 -23.75 1.27) ; 1, R-1-1-2-2-2-2-1-2-1-2-2 + ( -280.82 -906.63 -23.75 1.27) ; 2 + ( -284.62 -907.77 -23.75 1.27) ; 3 + ( -284.87 -907.77 -23.75 1.27) ; 4 + ( -288.03 -908.53 -23.75 1.27) ; 5 + ( -288.16 -908.53 -23.75 1.27) ; 6 + ( -290.31 -908.78 -23.75 1.27) ; 7 + ( -290.69 -908.78 -23.75 1.27) ; 8 + ( -293.60 -909.04 -23.75 1.27) ; 9 + ( -293.73 -909.04 -23.75 1.27) ; 10 + ( -296.39 -909.29 -23.75 1.14) ; 11 + ( -298.79 -910.05 -23.75 1.14) ; 12 + ( + ( -303.60 -911.69 -23.00 0.89) ; 1, R-1-1-2-2-2-2-1-2-1-2-2-1 + ( + ( -305.75 -913.59 -23.00 0.89) ; 1, R-1-1-2-2-2-2-1-2-1-2-2-1-1 + ( + ( -311.70 -916.25 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-1-2-2-1-1-1 + ( -311.58 -916.25 -23.25 0.89) ; 2 + 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-23.25 0.89) ; 13 + ( -331.20 -916.63 -23.25 0.89) ; 14 + ( -333.98 -916.88 -23.25 0.89) ; 15 + ( -334.24 -916.88 -23.25 0.89) ; 16 + ( -336.26 -917.14 -23.25 0.89) ; 17 + ( -336.51 -917.14 -23.25 0.89) ; 18 + Normal + ) ; End of split + | + ( -308.16 -912.71 -23.00 0.89) ; 1, R-1-1-2-2-2-2-1-2-1-2-2-1-1-2 + ( -310.69 -912.83 -23.00 0.89) ; 2 + ( -310.94 -912.83 -23.00 0.89) ; 3 + ( -312.34 -912.96 -23.00 0.89) ; 4 + ( -312.84 -913.21 -23.00 0.89) ; 5 + ( -313.60 -913.85 -23.00 0.89) ; 6 + Normal + ) ; End of split + | + ( -306.01 -910.43 -23.00 0.89) ; 1, R-1-1-2-2-2-2-1-2-1-2-2-1-2 + ( -308.16 -909.04 -23.00 0.89) ; 2 + ( -308.41 -908.78 -23.00 0.89) ; 3 + ( -310.18 -907.77 -23.00 0.89) ; 4 + ( + ( -311.32 -905.87 -23.00 0.89) ; 1, R-1-1-2-2-2-2-1-2-1-2-2-1-2-1 + ( -313.73 -904.23 -23.00 0.89) ; 2 + ( -313.98 -904.23 -23.00 0.89) ; 3 + ( -316.89 -903.85 -23.00 0.89) ; 4 + ( -317.15 -903.97 -23.00 0.89) ; 5 + ( -317.53 -903.97 -23.00 0.89) ; 6 + ( -320.82 -904.35 -23.00 0.89) ; 7 + ( 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1.01) ; 3 + ( -319.53 -907.57 -25.25 1.01) ; 4 + Normal + | + ( -318.77 -900.48 -24.75 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-1-2 + ( -319.28 -900.48 -24.75 1.01) ; 2 + ( -324.34 -900.48 -25.25 1.01) ; 3 + ( -324.59 -900.48 -25.25 1.01) ; 4 + ( -329.40 -901.75 -24.75 1.01) ; 5 + ( -332.69 -904.53 -24.50 1.01) ; 6 + ( -334.47 -907.57 -24.50 1.01) ; 7 + ( -334.47 -907.82 -24.50 1.01) ; 8 + Normal + ) ; End of split + | + ( -308.03 -893.59 -23.25 1.52) ; 1, R-1-1-2-2-2-2-1-2-2-2-2 + ( -308.16 -893.59 -23.25 1.52) ; 2 + ( -311.45 -893.47 -23.25 1.52) ; 3 + ( -314.62 -893.09 -23.25 1.52) ; 4 + ( + ( -315.50 -891.57 -23.25 0.63) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-1 + ( -316.64 -890.43 -23.25 0.63) ; 2 + ( -318.79 -888.91 -23.25 0.63) ; 3 + ( -320.44 -886.76 -23.25 0.63) ; 4 + ( -321.45 -885.11 -23.25 0.63) ; 5 + ( -321.45 -883.34 -23.25 0.63) ; 6 + Normal + | + ( -315.88 -893.72 -23.25 1.52) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2 + ( -318.41 -894.23 -23.25 1.52) ; 2 + ( -318.41 -894.35 -23.25 1.52) ; 3 + ( 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-867.95 -25.25 1.01) ; 21 + ( -358.39 -867.95 -25.25 1.01) ; 22 + ( -358.90 -867.82 -25.25 1.01) ; 23 + ( -359.53 -867.57 -25.25 1.01) ; 24 + ( -360.03 -867.57 -25.25 1.01) ; 25 + ( -360.54 -867.70 -25.25 1.01) ; 26 + ( -361.05 -867.82 -25.25 1.01) ; 27 + ( -361.81 -867.82 -25.25 1.01) ; 28 + ( -362.19 -867.95 -25.25 1.01) ; 29 + ( -363.20 -867.95 -25.25 1.01) ; 30 + ( -363.45 -868.07 -25.25 1.01) ; 31 + ( -363.58 -867.95 -25.25 1.01) ; 32 + ( -363.45 -867.82 -25.25 1.01) ; 33 + ( -363.45 -867.95 -25.25 1.01) ; 34 + ( + ( -364.34 -869.34 -24.75 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-1-1-1-2-1 + ( -364.59 -869.47 -24.75 1.01) ; 2 + ( -366.36 -869.72 -24.75 1.01) ; 3 + Normal + | + ( -364.09 -866.43 -25.25 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-1-1-1-2-2 + ( -364.59 -865.92 -25.25 1.01) ; 2 + ( -365.98 -863.77 -25.25 1.01) ; 3 + ( -365.86 -863.77 -25.25 1.01) ; 4 + Normal + ) ; End of split + ) ; End of split + | + ( -344.09 -877.19 -25.50 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-1-1-2 + ( 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0.76) ; 2 + ( -344.24 -880.17 -24.00 0.76) ; 3 + ( -344.49 -879.92 -24.00 0.76) ; 4 + ( -345.25 -879.42 -24.00 0.76) ; 5 + Normal + | + ( -342.97 -883.09 -24.00 0.76) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-1-2-2-2 + ( -345.75 -882.33 -24.00 0.76) ; 2 + ( -345.88 -882.20 -24.00 0.76) ; 3 + ( -347.40 -881.82 -24.00 0.76) ; 4 + ( -347.65 -881.82 -24.00 0.76) ; 5 + ( -349.30 -882.07 -24.00 0.76) ; 6 + ( -349.68 -882.07 -24.00 0.76) ; 7 + ( -350.94 -882.83 -24.00 0.76) ; 8 + ( -351.07 -882.96 -24.00 0.76) ; 9 + ( -351.96 -883.34 -24.00 0.76) ; 10 + ( -352.46 -883.34 -24.00 0.76) ; 11 + ( -355.12 -884.86 -24.00 0.76) ; 12 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -328.54 -895.87 -23.25 1.65) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2 + ( + ( -330.56 -895.36 -23.25 1.27) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-1 + ( -331.32 -895.36 -23.25 1.27) ; 2 + ( -331.45 -895.36 -23.25 1.27) ; 3 + ( + ( -333.48 -893.97 -23.25 1.14) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-1-1 + ( -334.62 -893.59 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; 4 + ( -355.88 -892.45 -23.25 1.01) ; 5 + ( -356.13 -892.45 -23.25 1.01) ; 6 + ( -357.27 -892.45 -23.25 1.01) ; 7 + ( -357.78 -892.58 -23.25 1.01) ; 8 + ( -358.54 -892.58 -23.25 1.01) ; 9 + ( -358.92 -892.58 -23.25 1.01) ; 10 + ( -358.92 -892.45 -23.25 1.01) ; 11 + ( -359.68 -892.07 -23.25 1.01) ; 12 + ( -359.93 -892.07 -23.25 1.01) ; 13 + ( -359.93 -891.95 -23.25 1.01) ; 14 + ( -360.69 -891.19 -23.25 1.01) ; 15 + Normal + | + ( -353.73 -895.74 -23.25 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-1-1-2-2 + ( -357.27 -895.24 -23.25 1.01) ; 2 + ( -357.78 -895.24 -23.25 1.01) ; 3 + ( -358.92 -894.86 -23.25 1.01) ; 4 + ( -359.30 -894.86 -23.25 1.01) ; 5 + ( -359.81 -894.86 -23.25 1.01) ; 6 + ( -360.56 -894.86 -23.25 1.01) ; 7 + ( -361.32 -894.61 -23.25 1.01) ; 8 + ( -361.96 -894.23 -23.25 1.01) ; 9 + ( -362.59 -893.59 -23.25 1.01) ; 10 + ( -362.97 -893.59 -23.25 1.01) ; 11 + ( -363.10 -893.47 -23.25 1.01) ; 12 + ( -363.22 -893.47 -23.25 1.01) ; 13 + ( -363.48 -893.47 -23.25 1.01) ; 14 + Normal + ) ; End of split + ) ; End of split + | + ( -333.86 -896.25 -23.25 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-1-2 + ( -335.12 -896.76 -23.25 1.01) ; 2 + ( -335.12 -896.88 -23.25 1.01) ; 3 + ( + ( -337.27 -896.63 -23.25 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-1-2-1 + ( -337.53 -896.63 -23.25 1.01) ; 2 + ( -340.94 -896.25 -23.25 1.01) ; 3 + ( -342.21 -896.88 -23.25 1.01) ; 4 + ( -342.46 -897.14 -23.25 1.01) ; 5 + ( -343.86 -897.77 -23.25 1.01) ; 6 + ( -344.11 -897.90 -23.25 1.01) ; 7 + ( -344.49 -898.02 -23.25 1.01) ; 8 + ( -345.63 -898.15 -23.25 1.01) ; 9 + ( -346.39 -898.15 -23.25 1.01) ; 10 + ( -346.77 -898.15 -23.25 1.01) ; 11 + ( -347.02 -898.28 -23.25 1.01) ; 12 + ( -348.79 -898.28 -23.25 1.01) ; 13 + ( -349.93 -898.28 -23.25 1.01) ; 14 + ( -350.69 -898.28 -23.25 1.01) ; 15 + ( -351.58 -898.28 -23.25 1.01) ; 16 + ( -352.59 -898.40 -23.25 1.01) ; 17 + ( -354.62 -897.90 -23.25 1.01) ; 18 + ( -354.87 -897.90 -23.25 1.01) ; 19 + ( -357.65 -897.26 -23.25 1.01) ; 20 + ( -358.03 -897.26 -23.25 1.01) ; 21 + ( -359.05 -897.14 -23.25 1.01) ; 22 + ( -359.81 -897.14 -23.25 1.01) ; 23 + ( -360.82 -897.26 -23.25 1.01) ; 24 + ( -361.58 -897.26 -23.25 1.01) ; 25 + ( -363.73 -897.39 -23.25 1.01) ; 26 + ( -364.62 -897.39 -23.25 1.01) ; 27 + ( -365.37 -897.39 -23.25 1.01) ; 28 + ( -366.01 -897.52 -23.25 1.01) ; 29 + ( -366.26 -897.52 -23.25 1.01) ; 30 + ( -367.27 -898.02 -23.25 1.01) ; 31 + ( -367.53 -898.02 -23.25 1.01) ; 32 + Normal + | + ( -337.78 -898.78 -23.25 1.01) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-1-2-2 + ( -337.91 -898.78 -23.25 1.01) ; 2 + ( -340.06 -899.42 -23.25 1.01) ; 3 + ( -340.44 -899.54 -23.25 1.01) ; 4 + ( -343.48 -900.30 -23.25 1.01) ; 5 + ( -343.73 -900.30 -23.25 1.01) ; 6 + ( -346.51 -900.43 -23.25 1.01) ; 7 + ( -347.15 -900.55 -23.25 1.01) ; 8 + ( -350.06 -900.81 -23.25 1.01) ; 9 + ( -350.44 -900.93 -23.25 1.01) ; 10 + ( -355.37 -901.31 -23.25 1.01) ; 11 + ( -355.63 -901.31 -23.25 1.01) ; 12 + ( -356.51 -901.31 -23.25 1.01) ; 13 + ( + ( -358.92 -900.05 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-900.43 -23.25 0.76) ; 6 + ( -366.51 -900.43 -23.25 0.76) ; 7 + ( -367.15 -900.68 -23.25 0.76) ; 8 + ( -367.91 -901.31 -23.25 0.76) ; 9 + ( -368.29 -901.31 -23.25 0.76) ; 10 + ( -368.41 -901.57 -23.25 0.76) ; 11 + ( -368.54 -901.57 -23.25 0.76) ; 12 + ( -368.67 -901.57 -23.25 0.76) ; 13 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -332.84 -899.54 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2 + ( -334.24 -900.68 -23.25 0.89) ; 2 + ( -334.36 -900.81 -23.25 0.89) ; 3 + ( -336.77 -901.82 -23.25 0.89) ; 4 + ( + ( -337.15 -906.00 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-1 + ( -337.40 -907.64 -23.25 0.89) ; 2 + ( -337.40 -907.90 -23.25 0.89) ; 3 + ( -337.53 -908.02 -23.25 0.89) ; 4 + ( -337.78 -909.29 -23.25 0.89) ; 5 + ( -337.91 -909.54 -23.25 0.89) ; 6 + Normal + | + ( -340.94 -903.47 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2 + ( -341.20 -903.47 -23.25 0.89) ; 2 + ( -344.62 -904.61 -23.25 0.89) ; 3 + ( -344.74 -904.73 -23.25 0.89) ; 4 + ( -346.64 -906.38 -23.25 0.89) ; 5 + ( + ( -351.20 -904.61 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-1 + ( -351.58 -904.61 -23.25 0.89) ; 2 + ( -355.00 -904.10 -23.25 0.89) ; 3 + ( + ( -358.79 -907.90 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-1-1 + ( -359.30 -908.91 -23.25 0.89) ; 2 + ( -359.43 -908.91 -23.25 0.89) ; 3 + Normal + | + ( -357.40 -903.72 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-1-2 + ( -357.65 -903.72 -23.25 0.89) ; 2 + ( + ( -360.69 -904.61 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-1-2-1 + ( -362.72 -904.10 -23.25 0.89) ; 2 + ( -364.74 -904.10 -23.25 0.89) ; 3 + ( -365.25 -904.10 -23.25 0.89) ; 4 + ( -366.77 -904.23 -23.25 0.89) ; 5 + Normal + ) ; End of split + ) ; End of split + | + ( -347.02 -907.26 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2 + ( + ( -345.63 -909.04 -23.25 0.63) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2-1 + ( -344.74 -910.30 -23.25 0.63) ; 2 + ( -344.74 -910.43 -23.25 0.63) ; 3 + ( 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0.89) ; 2 + ( -359.05 -922.45 -23.00 0.89) ; 3 + Normal + ) ; End of split + | + ( -359.68 -912.33 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2-2-2 + ( + ( -360.82 -911.06 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2-2-2-1 + ( -360.94 -910.93 -23.25 0.89) ; 2 + ( -364.24 -909.42 -23.25 0.89) ; 3 + ( -366.13 -909.42 -23.25 0.89) ; 4 + ( -366.89 -909.42 -23.25 0.89) ; 5 + ( -367.53 -909.54 -23.25 0.89) ; 6 + ( -367.65 -909.54 -23.25 0.89) ; 7 + ( -367.91 -909.67 -23.25 0.89) ; 8 + Normal + | + ( -361.83 -914.98 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2-2-2-2 + ( + ( -363.60 -914.10 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2-2-2-2-1 + ( -366.64 -913.72 -23.25 0.89) ; 2 + ( -367.40 -913.72 -23.25 0.89) ; 3 + ( -368.29 -913.85 -23.25 0.89) ; 4 + ( -368.54 -913.85 -23.25 0.89) ; 5 + ( -368.67 -913.97 -23.25 0.89) ; 6 + ( -369.05 -913.97 -23.25 0.89) ; 7 + ( -369.68 -913.97 -23.25 0.89) ; 8 + ( -369.93 -913.97 -23.25 0.89) ; 9 + ( -370.31 -914.10 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-924.73 -23.25 0.89) ; 4 + ( -362.21 -924.86 -23.25 0.89) ; 5 + ( -362.21 -924.86 -23.25 0.89) ; 6 + Normal + | + ( -367.53 -922.45 -23.25 0.89) ; 1, R-1-1-2-2-2-2-1-2-2-2-2-2-2-2-2-2-2-2-2-2-2-2-2-2 + ( -367.91 -922.83 -23.25 0.89) ; 2 + ( -368.03 -924.61 -23.25 0.89) ; 3 + ( -367.91 -925.11 -23.25 0.89) ; 4 + ( -367.91 -925.24 -23.25 0.89) ; 5 + ( -367.91 -925.36 -23.25 0.89) ; 6 + ( -367.78 -925.62 -23.25 0.89) ; 7 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -263.33 -897.96 -23.25 0.76) ; 1, R-1-1-2-2-2-2-2 + ( -262.95 -896.19 -23.25 0.76) ; 2 + ( -262.82 -895.94 -23.25 0.76) ; 3 + ( -262.82 -895.68 -23.25 0.76) ; 4 + ( -262.69 -895.56 -23.25 0.76) ; 5 + ( -262.69 -893.03 -23.25 0.76) ; 6 + ( 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-899.48 -22.75 0.76) ; 3 + ( -249.15 -899.99 -22.75 0.76) ; 4 + ( -249.02 -900.24 -22.75 0.76) ; 5 + ( -248.64 -900.62 -22.75 0.76) ; 6 + ( -248.52 -900.75 -22.75 0.76) ; 7 + ( -248.39 -900.87 -22.75 0.76) ; 8 + ( -248.26 -901.00 -22.75 0.76) ; 9 + ( -248.14 -901.00 -22.75 0.76) ; 10 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -228.91 -935.16 -25.00 1.01) ; 1, R-1-2 + ( -228.91 -935.42 -25.00 1.01) ; 2 + ( -224.86 -938.20 -25.00 1.01) ; 3 + ( -224.86 -938.45 -25.00 1.01) ; 4 + ( -220.56 -940.99 -25.50 1.01) ; 5 + ( -220.30 -941.24 -25.50 1.01) ; 6 + ( -214.73 -943.26 -25.50 1.01) ; 7 + ( -214.48 -943.77 -25.50 1.01) ; 8 + ( + ( -210.43 -944.28 -26.00 1.01) ; 1, R-1-2-1 + ( -209.92 -944.53 -26.00 1.01) ; 2 + ( -206.63 -942.76 -26.00 1.01) ; 3 + Normal + | + ( -213.97 -947.57 -25.75 1.01) ; 1, R-1-2-2 + ( -213.97 -947.82 -25.75 1.01) ; 2 + ( + ( -216.76 -950.35 -25.25 1.01) ; 1, R-1-2-2-1 + ( -216.76 -950.86 -25.25 1.01) ; 2 + Normal + | + ( -210.68 -949.85 -26.00 1.01) ; 1, R-1-2-2-2 + ( -210.43 -949.59 -26.00 1.01) ; 2 + ( + ( -206.38 -949.09 -26.00 1.01) ; 1, R-1-2-2-2-1 + ( -205.87 -948.83 -26.00 1.01) ; 2 + Normal + | + ( -209.92 -952.89 -26.00 1.01) ; 1, R-1-2-2-2-2 + ( -209.67 -953.39 -26.00 1.01) ; 2 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -234.12 -955.36 -25.75 1.90) ; 1, R-2 + ( -238.04 -956.49 -25.75 1.90) ; 2 + ( -242.35 -957.76 -25.75 1.90) ; 3 + ( -245.64 -959.91 -25.75 1.90) ; 4 + ( -248.04 -962.06 -25.75 1.90) ; 5 + ( -251.21 -963.96 -25.75 1.90) ; 6 + ( -253.48 -965.48 -25.75 1.90) ; 7 + ( -253.61 -965.61 -25.75 1.90) ; 8 + ( -257.54 -967.76 -25.75 1.90) ; 9 + ( -263.10 -973.96 -25.75 1.90) ; 10 + ( -265.64 -978.27 -25.75 1.90) ; 11 + ( -268.55 -983.58 -25.75 1.90) ; 12 + ( -268.67 -983.84 -25.75 1.90) ; 13 + ( -268.93 -984.22 -25.75 1.90) ; 14 + ( -272.85 -987.76 -25.75 1.90) ; 15 + ( 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-981.16 -24.25 0.76) ; 1, R-2-1-1-2-2-1 + ( -240.15 -981.16 -24.25 0.76) ; 2 + ( -240.02 -979.00 -24.25 0.76) ; 3 + ( + ( -237.87 -973.43 -24.25 0.76) ; 1, R-2-1-1-2-2-1-1 + ( -237.37 -969.51 -24.25 0.76) ; 2 + Normal + | + ( -237.11 -976.35 -24.25 0.76) ; 1, R-2-1-1-2-2-1-2 + ( -234.33 -973.43 -24.25 0.76) ; 2 + ( -232.43 -973.69 -24.25 0.76) ; 3 + ( -232.43 -973.94 -24.25 0.76) ; 4 + Normal + ) ; End of split + | + ( -240.53 -989.38 -24.25 0.76) ; 1, R-2-1-1-2-2-2 + ( -237.87 -991.28 -24.25 0.76) ; 2 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -268.38 -993.43 -24.25 0.89) ; 1, R-2-1-2 + ( + ( -269.90 -996.60 -24.25 0.63) ; 1, R-2-1-2-1 + ( -271.42 -1000.65 -24.25 0.63) ; 2 + ( + ( -267.75 -1004.45 -24.25 0.76) ; 1, R-2-1-2-1-1 + ( -265.85 -1006.35 -24.25 0.76) ; 2 + ( -265.59 -1006.98 -24.25 0.76) ; 3 + ( -264.20 -1008.75 -24.25 0.76) ; 4 + ( -263.95 -1009.13 -24.25 0.76) ; 5 + ( -262.94 -1010.78 -24.25 0.76) ; 6 + ( -262.68 -1011.28 -24.25 0.76) ; 7 + ( 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0.76) ; 21 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -275.21 -989.38 -24.25 2.66) ; 1, R-2-2 + ( -277.87 -990.78 -24.25 2.66) ; 2 + ( -280.53 -991.41 -24.25 2.66) ; 3 + ( -283.06 -991.91 -24.25 2.66) ; 4 + ( -283.19 -991.91 -24.25 2.66) ; 5 + ( -284.83 -992.55 -24.25 2.66) ; 6 + ( -286.10 -993.05 -24.25 2.66) ; 7 + ( + ( -288.51 -996.47 -25.00 1.77) ; 1, R-2-2-1 + ( -293.32 -998.62 -25.00 1.77) ; 2 + ( -294.83 -999.38 -25.00 1.77) ; 3 + ( + ( -296.86 -1001.79 -25.00 1.14) ; 1, R-2-2-1-1 + ( + ( -295.59 -1004.07 -25.00 1.01) ; 1, R-2-2-1-1-1 + ( -295.34 -1006.60 -25.00 1.01) ; 2 + ( -295.34 -1006.73 -25.00 1.01) ; 3 + ( -295.72 -1008.62 -25.00 1.01) ; 4 + ( -295.85 -1008.75 -25.00 1.01) ; 5 + ( -295.97 -1009.00 -25.00 1.01) ; 6 + ( -296.23 -1009.38 -25.00 1.01) ; 7 + Normal + | + ( -301.16 -1003.31 -25.00 1.01) ; 1, R-2-2-1-1-2 + ( + ( -303.44 -1005.71 -25.00 1.01) ; 1, R-2-2-1-1-2-1 + ( -303.95 -1007.99 -25.00 1.01) ; 2 + ( -303.95 -1008.24 -25.00 1.01) 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0.89) ; 6 + ( -323.06 -978.37 -25.00 0.89) ; 7 + Normal + | + ( -324.58 -990.14 -25.00 0.89) ; 1, R-2-2-1-2-1-2 + ( -326.23 -990.78 -25.00 0.89) ; 2 + ( -326.48 -990.90 -25.00 0.89) ; 3 + ( -327.75 -991.16 -25.00 0.89) ; 4 + ( -327.87 -991.16 -25.00 0.89) ; 5 + ( -328.51 -991.16 -25.00 0.89) ; 6 + ( -328.76 -991.16 -25.00 0.89) ; 7 + Normal + ) ; End of split + | + ( -317.49 -997.48 -25.00 0.89) ; 1, R-2-2-1-2-2 + ( + ( -318.63 -995.46 -25.00 0.89) ; 1, R-2-2-1-2-2-1 + ( -321.16 -994.95 -25.00 0.89) ; 2 + ( -323.82 -994.95 -25.00 0.89) ; 3 + ( -324.07 -994.95 -25.00 0.89) ; 4 + ( -325.09 -994.95 -25.00 0.89) ; 5 + ( -325.34 -994.95 -25.00 0.89) ; 6 + ( -326.23 -994.95 -25.00 0.89) ; 7 + ( -326.73 -994.95 -25.00 0.89) ; 8 + ( -327.87 -994.70 -25.00 0.89) ; 9 + ( -328.25 -994.70 -25.00 0.89) ; 10 + ( -328.51 -994.70 -25.00 0.89) ; 11 + ( -329.90 -993.94 -25.00 0.89) ; 12 + ( + ( -331.42 -992.42 -25.00 0.89) ; 1, R-2-2-1-2-2-1-1 + ( + ( -332.81 -991.91 -25.00 0.89) ; 1, R-2-2-1-2-2-1-1-1 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-989.76 -25.00 0.89) ; 11 + ( -349.39 -990.14 -25.00 0.89) ; 12 + ( -349.90 -990.14 -25.00 0.89) ; 13 + ( -350.91 -990.40 -25.00 0.89) ; 14 + ( -351.04 -990.52 -25.00 0.89) ; 15 + Normal + ) ; End of split + | + ( -332.81 -987.74 -25.00 0.89) ; 1, R-2-2-1-2-2-1-1-2 + ( -334.07 -986.85 -25.00 0.89) ; 2 + ( -334.83 -986.72 -25.00 0.89) ; 3 + ( -335.47 -986.60 -25.00 0.89) ; 4 + ( -335.97 -986.35 -25.00 0.89) ; 5 + ( -336.35 -986.22 -25.00 0.89) ; 6 + ( -336.86 -985.97 -25.00 0.89) ; 7 + Normal + ) ; End of split + | + ( -332.18 -995.46 -25.00 0.89) ; 1, R-2-2-1-2-2-1-2 + ( -332.18 -995.59 -25.00 0.89) ; 2 + ( -334.20 -996.98 -25.00 0.89) ; 3 + ( + ( -335.47 -997.74 -25.00 0.89) ; 1, R-2-2-1-2-2-1-2-1 + ( -335.85 -997.99 -25.00 0.89) ; 2 + ( -336.73 -999.00 -25.00 0.89) ; 3 + ( -337.11 -999.13 -25.00 0.89) ; 4 + ( -337.75 -999.13 -25.00 0.89) ; 5 + ( -338.51 -999.13 -25.00 0.89) ; 6 + Normal + | + ( -337.75 -995.97 -25.00 0.89) ; 1, R-2-2-1-2-2-1-2-2 + ( -338.51 -995.97 -25.00 0.89) ; 2 + 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; 10 + ( -361.66 -1005.44 -27.00 0.64) ; 11 + ( -361.82 -1005.60 -27.00 0.64) ; 12 + ( -361.98 -1005.76 -27.00 0.64) ; 13 + ( -362.13 -1006.08 -27.00 0.64) ; 14 + ( -362.29 -1006.23 -27.00 0.64) ; 15 + ( -362.45 -1006.39 -27.00 0.64) ; 16 + ( -362.61 -1006.71 -27.00 0.64) ; 17 + ( -362.77 -1006.87 -27.00 0.64) ; 18 + ( -362.77 -1007.35 -27.00 0.64) ; 19 + ( -362.93 -1007.51 -27.00 0.64) ; 20 + ( -362.93 -1007.83 -27.00 0.64) ; 21 + ( + ( -365.64 -1008.62 -27.00 0.64) ; 1, R-2-2-1-2-2-2-2-1-1 + ( -365.96 -1008.78 -27.00 0.64) ; 2 + ( -369.78 -1010.53 -27.00 0.64) ; 3 + ( -369.78 -1010.69 -27.00 0.64) ; 4 + ( -370.04 -1010.64 -27.00 0.64) ; 5 + ( + ( -370.84 -1012.07 -26.75 1.11) ; 1, R-2-2-1-2-2-2-2-1-1-1 + ( -371.00 -1012.39 -26.75 1.11) ; 2 + ( -373.54 -1014.94 -26.75 1.11) ; 3 + ( -373.70 -1015.10 -26.75 1.11) ; 4 + ( -377.36 -1017.16 -26.75 1.11) ; 5 + ( -377.52 -1017.48 -26.75 1.11) ; 6 + ( -380.23 -1019.71 -26.75 1.11) ; 7 + Normal + | + ( -371.79 -1010.64 -27.00 0.80) ; 1, 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-1001.14 -27.50 1.01) ; 21 + ( -378.52 -1000.88 -27.50 1.01) ; 22 + ( -378.26 -1001.14 -27.50 1.01) ; 23 + Normal + | + ( -356.99 -996.47 -25.75 0.89) ; 1, R-2-2-1-2-2-2-2-2-2 + ( -358.13 -994.83 -25.75 0.89) ; 2 + ( -359.90 -993.31 -25.75 0.89) ; 3 + ( -360.28 -993.18 -25.75 0.89) ; 4 + ( -362.57 -992.27 -27.25 1.01) ; 5 + ( -364.34 -992.02 -27.25 1.01) ; 6 + ( -364.09 -992.02 -27.25 1.01) ; 7 + ( + ( -366.62 -994.55 -28.75 1.01) ; 1, R-2-2-1-2-2-2-2-2-2-1 + ( -366.87 -994.81 -28.75 1.01) ; 2 + ( -371.17 -996.07 -28.75 1.01) ; 3 + ( -373.20 -997.09 -29.75 1.01) ; 4 + Normal + | + ( -366.87 -990.00 -27.25 1.01) ; 1, R-2-2-1-2-2-2-2-2-2-2 + ( -367.38 -990.00 -27.25 1.01) ; 2 + ( -371.68 -991.52 -27.25 1.01) ; 3 + ( -371.93 -991.77 -27.25 1.01) ; 4 + ( -377.50 -993.79 -27.25 1.01) ; 5 + ( -377.76 -993.79 -27.25 1.01) ; 6 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -288.78 -990.86 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split + | + ( -291.69 -988.58 -24.25 1.90) ; 1, R-2-2-2-2 + ( -292.95 -987.44 -24.25 1.90) ; 2 + ( + ( -296.24 -989.09 -24.25 1.01) ; 1, R-2-2-2-2-1 + ( -298.14 -989.34 -24.25 1.01) ; 2 + ( -298.52 -989.34 -24.25 1.01) ; 3 + ( -301.31 -989.47 -24.25 1.01) ; 4 + ( -301.69 -989.47 -24.25 1.01) ; 5 + ( -305.23 -989.47 -24.25 1.01) ; 6 + ( + ( -307.76 -990.10 -24.25 1.01) ; 1, R-2-2-2-2-1-1 + ( + ( -310.04 -991.62 -24.25 1.01) ; 1, R-2-2-2-2-1-1-1 + ( -310.17 -991.75 -24.25 1.01) ; 2 + ( -311.69 -994.15 -24.25 1.01) ; 3 + ( -311.94 -994.41 -24.25 1.01) ; 4 + ( -312.83 -995.29 -24.25 1.01) ; 5 + Normal + | + ( -311.81 -989.60 -24.25 0.63) ; 1, R-2-2-2-2-1-1-2 + ( -312.19 -989.60 -24.25 0.63) ; 2 + ( -313.33 -989.72 -24.25 0.63) ; 3 + ( -313.59 -989.85 -24.25 0.63) ; 4 + ( -314.60 -989.72 -24.25 0.63) ; 5 + ( -315.23 -989.34 -24.25 0.63) ; 6 + ( -316.37 -989.09 -24.25 0.63) ; 7 + ( -316.75 -988.96 -24.25 0.63) ; 8 + ( -317.00 -988.71 -24.25 0.63) ; 9 + ( -318.14 -988.58 -24.25 0.63) ; 10 + ( 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split + | + ( -309.03 -987.19 -24.25 0.63) ; 1, R-2-2-2-2-1-2-2 + ( -311.05 -987.19 -24.25 0.63) ; 2 + ( -311.81 -987.19 -24.25 0.63) ; 3 + ( -313.21 -986.94 -24.25 0.63) ; 4 + ( -313.96 -986.81 -24.25 0.63) ; 5 + ( -315.10 -986.68 -24.25 0.63) ; 6 + ( -316.24 -986.68 -24.25 0.63) ; 7 + ( -316.50 -986.56 -24.25 0.63) ; 8 + ( -316.88 -986.30 -24.25 0.63) ; 9 + ( -317.38 -986.30 -24.25 0.63) ; 10 + ( -319.03 -985.67 -24.25 0.63) ; 11 + ( -319.28 -985.67 -24.25 0.63) ; 12 + ( -321.56 -985.67 -24.25 0.63) ; 13 + ( -321.94 -985.80 -24.25 0.63) ; 14 + ( -323.21 -985.93 -24.25 0.63) ; 15 + ( -323.46 -985.93 -24.25 0.63) ; 16 + ( -323.71 -985.93 -24.25 0.63) ; 17 + ( -323.96 -985.93 -24.25 0.63) ; 18 + ( -324.09 -985.93 -24.25 0.63) ; 19 + ( + ( -326.12 -984.41 -24.25 0.63) ; 1, R-2-2-2-2-1-2-2-1 + ( -326.24 -984.41 -24.25 0.63) ; 2 + ( -328.02 -983.39 -24.25 0.63) ; 3 + ( -328.14 -983.52 -24.25 0.63) ; 4 + ( -330.17 -984.79 -24.25 0.63) ; 5 + ( -330.55 -985.04 -24.25 0.63) ; 6 + ( -331.05 -985.29 -24.25 0.63) ; 7 + ( -331.69 -985.55 -24.25 0.63) ; 8 + ( -332.07 -985.67 -24.25 0.63) ; 9 + ( -332.70 -985.67 -24.25 0.63) ; 10 + ( -334.34 -985.80 -24.25 0.63) ; 11 + ( -334.60 -985.93 -24.25 0.63) ; 12 + ( -334.72 -985.93 -24.25 0.63) ; 13 + ( -335.36 -986.18 -24.25 0.63) ; 14 + ( -336.24 -986.56 -24.25 0.63) ; 15 + ( -337.38 -986.94 -24.25 0.63) ; 16 + Normal + | + ( -326.24 -987.44 -24.25 0.63) ; 1, R-2-2-2-2-1-2-2-2 + ( -327.38 -988.46 -24.25 0.63) ; 2 + ( -327.89 -988.71 -24.25 0.63) ; 3 + ( -327.89 -988.84 -24.25 0.63) ; 4 + ( -328.52 -988.96 -24.25 0.63) ; 5 + ( -329.03 -989.09 -24.25 0.63) ; 6 + ( -329.15 -989.09 -24.25 0.63) ; 7 + ( -329.66 -989.09 -24.25 0.63) ; 8 + ( -329.79 -989.09 -24.25 0.63) ; 9 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + | + ( -292.95 -987.06 -24.25 1.90) ; 1, R-2-2-2-2-2 + ( + ( -290.80 -984.53 -24.25 1.14) ; 1, R-2-2-2-2-2-1 + ( -289.91 -982.89 -24.25 1.14) ; 2 + ( -289.15 -981.75 -24.25 1.14) ; 3 + ( -289.15 -981.37 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-981.75 -24.25 0.63) ; 8 + ( -347.38 -981.75 -24.25 0.63) ; 9 + ( -349.79 -982.25 -24.25 0.63) ; 10 + ( -350.80 -982.25 -24.25 0.63) ; 11 + ( -352.57 -982.25 -24.25 0.63) ; 12 + ( -353.21 -982.13 -24.25 0.63) ; 13 + ( -353.46 -981.87 -24.25 0.63) ; 14 + ( -354.34 -981.75 -24.25 0.63) ; 15 + ( -354.72 -981.49 -24.25 0.63) ; 16 + ( -355.36 -980.99 -24.25 0.63) ; 17 + ( -356.75 -980.99 -24.25 0.63) ; 18 + ( -357.38 -980.99 -24.25 0.63) ; 19 + ( -358.40 -980.99 -24.25 0.63) ; 20 + ( -358.90 -980.99 -24.25 0.63) ; 21 + ( -360.29 -981.12 -24.25 0.63) ; 22 + ( -361.31 -981.24 -24.25 0.63) ; 23 + ( -361.43 -981.24 -24.25 0.63) ; 24 + ( -362.45 -981.87 -24.25 0.63) ; 25 + ( -362.57 -982.13 -24.25 0.63) ; 26 + ( -363.59 -982.13 -24.25 0.63) ; 27 + ( -364.09 -982.25 -24.25 0.63) ; 28 + ( -364.34 -982.25 -24.25 0.63) ; 29 + ( -364.98 -982.38 -24.25 0.63) ; 30 + ( -364.98 -982.51 -24.25 0.63) ; 31 + ( -366.12 -982.63 -24.25 0.63) ; 32 + ( -366.50 -983.01 -24.25 0.63) ; 33 + ( -366.62 -983.01 -24.25 0.63) ; 34 + Normal + | + ( -336.50 -976.05 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2 + ( -340.42 -974.79 -24.25 0.63) ; 2 + ( -340.55 -974.79 -24.25 0.63) ; 3 + ( -342.32 -974.66 -24.25 0.63) ; 4 + ( + ( -343.33 -973.65 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-1 + ( -344.85 -971.37 -24.25 0.63) ; 2 + ( -346.50 -970.23 -24.25 0.63) ; 3 + ( -346.62 -970.23 -24.25 0.63) ; 4 + ( -349.79 -969.09 -24.25 0.63) ; 5 + ( -350.67 -969.09 -24.25 0.63) ; 6 + ( -352.32 -968.96 -24.25 0.63) ; 7 + ( -353.59 -968.96 -24.25 0.63) ; 8 + ( -354.34 -968.96 -24.25 0.63) ; 9 + ( -354.47 -968.96 -24.25 0.63) ; 10 + ( -355.74 -968.84 -24.25 0.63) ; 11 + ( + ( -356.12 -967.82 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-1-1 + ( -356.24 -967.82 -24.25 0.63) ; 2 + ( -359.79 -966.05 -24.25 0.63) ; 3 + ( -360.29 -966.05 -24.25 0.63) ; 4 + ( -363.59 -966.05 -24.25 0.63) ; 5 + ( -363.97 -966.05 -24.25 0.63) ; 6 + Normal + | + ( -358.14 -971.12 -24.75 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-1-2 + ( -358.52 -971.12 -24.75 0.63) ; 2 + ( -361.81 -970.36 -24.75 0.63) ; 3 + ( -362.70 -970.10 -24.75 0.63) ; 4 + ( -364.60 -968.58 -24.75 0.63) ; 5 + ( -364.72 -968.58 -24.75 0.63) ; 6 + ( -365.61 -967.70 -24.75 0.63) ; 7 + Normal + ) ; End of split + | + ( -345.99 -975.55 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-2 + ( + ( -349.41 -974.53 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-2-1 + ( -349.91 -974.53 -24.25 0.63) ; 2 + ( -351.56 -974.03 -24.25 0.63) ; 3 + ( -351.81 -973.77 -24.25 0.63) ; 4 + ( -352.32 -973.39 -24.25 0.63) ; 5 + ( -352.45 -973.27 -24.25 0.63) ; 6 + ( -355.86 -973.01 -24.25 0.63) ; 7 + ( -356.50 -973.01 -24.25 0.63) ; 8 + ( -356.75 -973.01 -24.25 0.63) ; 9 + Normal + | + ( -349.15 -977.44 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-2-2 + ( -349.28 -977.44 -24.25 0.63) ; 2 + ( -353.08 -977.44 -24.25 0.63) ; 3 + ( -353.84 -977.19 -24.25 0.63) ; 4 + ( -355.36 -976.81 -24.25 0.63) ; 5 + ( + ( -359.03 -976.94 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-2-2-1 + ( -359.66 -976.94 -24.25 0.63) ; 2 + ( -362.19 -977.06 -24.25 0.63) ; 3 + ( -362.32 -977.06 -24.25 0.63) ; 4 + ( -362.95 -977.06 -24.25 0.63) ; 5 + ( -363.08 -977.06 -24.25 0.63) ; 6 + ( -365.23 -974.66 -24.25 0.63) ; 7 + ( -365.36 -974.41 -24.25 0.63) ; 8 + ( -367.13 -974.15 -24.25 0.63) ; 9 + ( -367.51 -974.28 -24.25 0.63) ; 10 + ( -367.76 -974.41 -24.25 0.63) ; 11 + ( -370.67 -977.19 -24.25 0.63) ; 12 + ( -370.80 -977.44 -24.25 0.63) ; 13 + ( -371.31 -977.82 -24.25 0.63) ; 14 + ( -371.69 -978.08 -24.25 0.63) ; 15 + ( -371.81 -978.20 -24.25 0.63) ; 16 + ( -372.83 -978.96 -24.25 0.63) ; 17 + ( -373.21 -979.09 -24.25 0.63) ; 18 + Normal + | + ( -360.17 -974.15 -24.25 0.63) ; 1, R-2-2-2-2-2-2-2-2-2-2-2-2-2-2-2 + ( -362.95 -972.76 -24.25 0.63) ; 2 + ( -363.21 -972.63 -24.25 0.63) ; 3 + ( -366.62 -971.24 -24.25 0.63) ; 4 + ( -367.51 -971.24 -24.25 0.63) ; 5 + ( -369.28 -971.49 -24.25 0.63) ; 6 + ( -369.66 -971.62 -24.25 0.63) ; 7 + ( -370.04 -971.62 -24.25 0.63) ; 8 + ( -370.80 -972.00 -24.25 0.63) ; 9 + ( -371.31 -972.13 -24.25 0.63) ; 10 + ( -371.43 -972.13 -24.25 0.63) ; 11 + ( -372.70 -972.13 -24.25 0.63) ; 12 + ( -373.71 -971.87 -24.25 0.63) ; 13 + ( -373.84 -971.75 -24.25 0.63) ; 14 + Normal + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split + ) ; End of split +) ; End of tree diff --git a/docs/tutorial/01_opt_external_models/run_bionet.added_channels.py b/docs/tutorial/01_opt_external_models/run_bionet.added_channels.py new file mode 100755 index 00000000..bef0b614 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/run_bionet.added_channels.py @@ -0,0 +1,37 @@ +# -*- coding: utf-8 -*- +import os, sys +from pprint import pprint +from neuron import h + +from bmtk.simulator import bionet +from bmtk.simulator.bionet.io_tools import io + + +@bionet.model_processing +def add_channels(hoc_obj, cell, dynamics_params): + for sec in hoc_obj.all: + if 'axon' in sec.name(): + continue + + sec.insert('iahp') + setattr(sec, 'gkbar_iahp', 3.0e-04) + setattr(sec, 'taum_iahp', 0.5) + + return hoc_obj + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + run(sys.argv[-1]) + else: + run('config.added_channels.json') diff --git a/docs/tutorial/01_opt_external_models/run_bionet.ball_and_stick.py b/docs/tutorial/01_opt_external_models/run_bionet.ball_and_stick.py new file mode 100644 index 00000000..742f1678 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/run_bionet.ball_and_stick.py @@ -0,0 +1,75 @@ +"""Simulates an example network of 450 cells receiving two kinds of external input as defined in the configuration file""" +import os +import sys +from bmtk.simulator import bionet +from bmtk.simulator.bionet.io_tools import io + +from neuron import h + + +class BallAndStick: + def __init__(self, cell): + self._gid = cell['node_id'] + self._setup_morphology(cell) + self._setup_biophysics(cell) + + def _setup_morphology(self, cell): + self.soma = h.Section(name='soma', cell=self) + self.dend = h.Section(name='dend', cell=self) + self.all = [self.soma, self.dend] + self.dend.connect(self.soma) + self.soma.L = self.soma.diam = 12.6157 + self.dend.L = 200 + self.dend.diam = 1 + + def _setup_biophysics(self, cell): + for sec in self.all: + sec.Ra = 100 # Axial resistance in Ohm * cm + sec.cm = 1 # Membrane capacitance in micro Farads / cm^2 + self.soma.insert('hh') + for seg in self.soma: + seg.hh.gnabar = cell['gnabar'] # Sodium conductance in S/cm2 + seg.hh.gkbar = cell['gkbar'] # Potassium conductance in S/cm2 + seg.hh.gl = cell['gl'] # Leak conductance in S/cm2 + seg.hh.el = cell['el'] # Reversal potential in mV + # Insert passive current in the dendrite # <-- NEW + self.dend.insert('pas') # <-- NEW + for seg in self.dend: # <-- NEW + seg.pas.g = cell['g_pas'] # Passive conductance in S/cm2 # <-- NEW + seg.pas.e = cell['e_pas'] # Leak reversal potential mV # <-- NEW + + def __repr__(self): + return 'BallAndStick[{}]'.format(self._gid) + + +@bionet.cell_model(directive='python:loadBAS', model_type='biophysical') +def loadPurkinjeModel(cell, template_name, dynamics_params): + print('HERE') + bas_cell = BallAndStick(cell) + return bas_cell + # soma = h.Section(name='soma', cell=self) + # self.dend = h.Section(name='dend', cell=self) + # exit() + # io.log_info(f'Loading cell {cell.node_id}, tempalate {template_name}, with spines_on={cell["spines_on"]}') + # cell = Purkinje_Morpho_1(cell['spines_on']) + # return cell + + +def run(config_path): + conf = bionet.Config.from_json(config_path, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + # run('config.simulation_syns.json') + run('config.simulation_iclamp.json') + diff --git a/docs/tutorial/01_opt_external_models/run_bionet.modified_densities.py b/docs/tutorial/01_opt_external_models/run_bionet.modified_densities.py new file mode 100755 index 00000000..ce21b799 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/run_bionet.modified_densities.py @@ -0,0 +1,49 @@ +# -*- coding: utf-8 -*- +import os, sys +from pprint import pprint +from neuron import h + +from bmtk.simulator import bionet +from bmtk.simulator.bionet.io_tools import io + + +@bionet.model_processing +def adjust_densities(hoc_obj, cell, dynamics_params): + n_segs = 0 + n_segs_updated = 0 + for sec in hoc_obj.apic: + for seg in sec: + n_segs += 1 + if h.distance(seg) > 1000: + n_segs_updated += 1 + org_cond = getattr(seg, 'gbar_Ih') + new_cond = org_cond*0.5 + setattr(seg, 'gbar_Ih', new_cond) + + print(f'{n_segs_updated} out of {n_segs} apical dendritic segments updated.') + return hoc_obj + + +# @bionet.model_processing(name='adjust_densities') +# def check_mechanisms(hoc_obj, cell, dynamics_params): +# for sec in hoc_obj.apic: +# print(sec.name()) +# pprint(sec.psection()) +# break + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + run(sys.argv[-1]) + else: + run('config.simulation.json') diff --git a/docs/tutorial/01_opt_external_models/run_bionet.purkinje.py b/docs/tutorial/01_opt_external_models/run_bionet.purkinje.py new file mode 100644 index 00000000..15cb46d6 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/run_bionet.purkinje.py @@ -0,0 +1,34 @@ +"""Simulates an example network of 450 cells receiving two kinds of external input as defined in the configuration file""" +import os +import sys +from bmtk.simulator import bionet +from bmtk.simulator.bionet.io_tools import io + +from Purkinje_morpho_1 import Purkinje_Morpho_1 + + +@bionet.cell_model(directive='python:Purkinje_morph_1', model_type='biophysical') +def loadPurkinjeModel(cell, template_name, dynamics_params): + io.log_info(f'Loading cell {cell.node_id}, tempalate {template_name}, with spines_on={cell["spines_on"]}') + cell = Purkinje_Morpho_1(cell['spines_on']) + return cell + + +def run(config_path): + conf = bionet.Config.from_json(config_path, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + # run('config.simulation_syns.json') + run('config.simulation_iclamp.json') + diff --git a/docs/tutorial/01_opt_external_models/run_bionet.py b/docs/tutorial/01_opt_external_models/run_bionet.py new file mode 100755 index 00000000..78af3307 --- /dev/null +++ b/docs/tutorial/01_opt_external_models/run_bionet.py @@ -0,0 +1,20 @@ +# -*- coding: utf-8 -*- +import os, sys +from bmtk.simulator import bionet + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + run(sys.argv[-1]) + else: + run('/local1/workspace/Notebooks/bmtk-workshop/opt_external_models/config.hay_syns.json') diff --git a/docs/tutorial/02_opt_nest_custom_models/Ch_NEST_Cusom_Models.ipynb b/docs/tutorial/02_opt_nest_custom_models/Ch_NEST_Cusom_Models.ipynb new file mode 100644 index 00000000..6f7000e4 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/Ch_NEST_Cusom_Models.ipynb @@ -0,0 +1,1245 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "386a5424-30e2-4e40-ac4e-547846da1294", + "metadata": {}, + "source": [ + "# Extending PointNet Networks and Simulation with External and Customized Models.\n", + "\n", + "In this tutorial, we explain through a series of examples how to use and simulate built-in NEST cell and synaptic models, and create and import custom models using NESTML, as well as ways to dynamically change cells parametters before each simulation.\n", + "\n", + "**Note** - scripts and files for running this tutorial can be found in the directory [02_opt_nest_custom_models/](https://github.com/AllenInstitute/bmtk/tree/develop/docs/tutorial/02_opt_nest_custom_models)" + ] + }, + { + "cell_type": "markdown", + "id": "0ad93be7-1947-481c-8975-79438bd04593", + "metadata": {}, + "source": [ + "---\n", + "\n", + "## Contents\n", + "1. [Example: Using built-in NEST models](#example-advance-icalmp)\n", + "2. [Example: Custom cell models with NESTML](#example-nestml)\n", + "3. [Example: Overriding the creation of cell models](#example-cell-creation)\n", + "4. [Example: Switching between synaptic models](#example-switching-models)\n", + "\n", + "---" + ] + }, + { + "cell_type": "markdown", + "id": "b24846e8-de07-4d2e-bd41-ea2b62c64ad3", + "metadata": {}, + "source": [ + "## 1. Example: Using Built-In NEST Models " + ] + }, + { + "cell_type": "markdown", + "id": "e9d5cd7c-b786-424e-9cfe-f51cf0e203c1", + "metadata": {}, + "source": [ + "PointNet utlizes the NEST simulator for running point-neuron simulations, which includes many different varieties and types of neuronal models the majority of which can readily used during simulation in BMTK. An very useful practice is to take an existing network model built on model of a cell and replace it with a different cell model that more closely fits the needs of our research. With BMTK and SONATA this can be easily done, often without any programming, and even done on the fly before any given simulation.\n", + "\n", + "A list of available models built into the NEST simulator can be found [here](https://nest-simulator.readthedocs.io/en/stable/models/index.html).\n", + "\n", + "Most of the spiking models will work out-of-the-box in BMTK, readily swapped in and out of existing simulations, and you can use multiple models in the same network. (*We will ignore rates-based models which can only tentively work with BMTK. And for devices like genators and recorders PointNet uses a separate way of inserting them into the simulation*). To define what model will be used with a specific subset of cells and their properties, SONATA has two defined reserved attributes `model_template` and `dynamics_params` which we will take advantage of in this example." + ] + }, + { + "cell_type": "markdown", + "id": "9de5ab45-f067-4b56-ab29-cd01569ab5c1", + "metadata": {}, + "source": [ + "### Setting the model type in our network." + ] + }, + { + "cell_type": "markdown", + "id": "13ad2a5b-6d0d-450b-b78e-e75806477644", + "metadata": {}, + "source": [ + "The default way to set the model type for subset of neurons (which may be just a single neuron) that all use the same built-in NEST model type is to set the the **model_type** attributes to a value `nest:` where `` is the name of one of the spiking [NEST cell models](https://nest-simulator.readthedocs.io/en/stable/models/index.html). However, different models will have different parameters. There are multiple ways to set model parameters in BMTK, but the one that is most flexible and will make it easier to switch out different models while testing and optimizing our network is to set the **dynamics_params** to an easy to edit json file.\n", + "\n", + "In the below example (*build_network.built_in.py*) will built a simple 100 cell network with synaptic stimuli. Initialally we will use [izhikevich](https://nest-simulator.readthedocs.io/en/stable/models/izhikevich.html) models using default params (**hint**: if the dynamics_params .json file is just an empty json file, then the cells will be loaded with the default params. This is a useful starting point). But you can change the **nest_model** (and optionally the **dynamics_params**) variable below to build the same network using different model types" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "8dfcdc1f-8e52-459c-82ba-819e4304e7fc", + "metadata": {}, + "outputs": [], + "source": [ + "nest_model = 'nest:izhikevich'\n", + "# nest_model = 'nest:iaf_psc_delta'\n", + "# nest_model = 'nest:aeif_cond_alpha'\n", + "# nest_model = 'nest:glif_psc'\n", + "\n", + "dynamics_params = 'custom_model_params.default.json'\n", + "# dynamics_params = 'custom_model_params.izhikevich.json'\n", + "# dynamics_params = 'custom_model_params.aeif_cond_alpha.json'\n", + "# dynamics_params = 'custom_model_params.glif_psc.json'" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "560a5df1-dc8c-4884-bc6c-077478b6f72e", + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "[OSXLTC5747Y:45465] shmem: mmap: an error occurred while determining whether or not /var/folders/pr/_10c7kr546b1t7cf9z259z_40000gp/T//ompi.OSXLTC5747Y.502/jf.0/3216834560/sm_segment.OSXLTC5747Y.502.bfbd0000.0 could be created.\n" + ] + } + ], + "source": [ + "import numpy as np\n", + "from bmtk.builder import NetworkBuilder\n", + "\n", + "\n", + "net = NetworkBuilder('net')\n", + "net.add_nodes(\n", + " N=100,\n", + " model_type='point_neuron',\n", + " model_template=nest_model,\n", + " dynamics_params=dynamics_params\n", + ")\n", + "\n", + "net.add_edges(\n", + " source=net.nodes(), target=net.nodes(),\n", + " connection_rule=1,\n", + " syn_weight=2.0,\n", + " delay=1.5,\n", + " dynamics_params='ExcToInh.json',\n", + " model_template='static_synapse'\n", + ")\n", + "\n", + "net.build()\n", + "net.save(output_dir='network_built_in')\n", + "\n", + "\n", + "virt_exc = NetworkBuilder('virt_exc')\n", + "virt_exc.add_nodes(\n", + " N=10,\n", + " model_type='virtual'\n", + ")\n", + "\n", + "virt_exc.add_edges(\n", + " target=net.nodes(),\n", + " connection_rule=lambda *_: np.random.randint(0, 10),\n", + " syn_weight=2.0,\n", + " delay=1.0,\n", + " dynamics_params='ExcToInh.json',\n", + " model_template='static_synapse'\n", + ")\n", + "\n", + "virt_exc.build()\n", + "virt_exc.save(output_dir='network_built_in')" + ] + }, + { + "cell_type": "markdown", + "id": "2ced04e1-511f-4e17-8c26-d8da389a7236", + "metadata": {}, + "source": [ + "If you have access to an existing model but not the build-script, or if rebuilding the network is too time consuming and/or expensive, then another option to swap out different models is to open the *node_types.csv* in an text editor (especially ones that support csv forats like VSCode or Atom), update the **model_template** and **dynamics_params** attributes, then rerun the simulation." + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "4167ebf8-4802-4c2c-8ac7-fea1cabd5505", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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0100custom_model_params.default.jsonpoint_neuronnest:izhikevich
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" + ], + "text/plain": [ + " node_type_id dynamics_params model_type \\\n", + "0 100 custom_model_params.default.json point_neuron \n", + "\n", + " model_template \n", + "0 nest:izhikevich " + ] + }, + "execution_count": 3, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "pd.read_csv('network_built_in/net_node_types.csv', sep=' ')" + ] + }, + { + "cell_type": "markdown", + "id": "6e0700fb-674e-40d9-bbec-b49697e4e9e9", + "metadata": {}, + "source": [ + "We encourage you to try running the network yourself by either running in the command line or un\n", + "```\n", + "$ python run_pointnet.built_in.py config.built_in.json\n", + "```\n", + "or with the below cell, trying it with different cell models. " + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "3aa1cd9b-0b93-4d33-b0d8-89ea13cff66c", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "\n", + " -- N E S T --\n", + " Copyright (C) 2004 The NEST Initiative\n", + "\n", + " Version: 3.7.0\n", + " Built: May 24 2024 10:13:36\n", + "\n", + " This program is provided AS IS and comes with\n", + " NO WARRANTY. See the file LICENSE for details.\n", + "\n", + " Problems or suggestions?\n", + " Visit https://www.nest-simulator.org\n", + "\n", + " Type 'nest.help()' to find out more about NEST.\n", + "\n", + "2024-10-21 15:11:35,805 [INFO] Created log file\n", + "2024-10-21 15:11:35,813 [INFO] Batch processing nodes for net/0.\n", + "2024-10-21 15:11:35,816 [INFO] Batch processing nodes for virt_exc/0.\n", + "2024-10-21 15:11:35,827 [INFO] Setting up output directory\n", + "2024-10-21 15:11:35,827 [INFO] Building cells.\n", + "2024-10-21 15:11:35,830 [INFO] Building recurrent connections\n", + "2024-10-21 15:11:35,834 [INFO] Network created.\n", + "2024-10-21 15:11:35,834 [INFO] Build virtual cell stimulations for thalamus_spikes\n", + "2024-10-21 15:11:35,844 [INFO] Starting Simulation\n", + "2024-10-21 15:11:39,062 [INFO] Simulation finished, finalizing results.\n", + "2024-10-21 15:11:39,351 [INFO] Done.\n" + ] + } + ], + "source": [ + "from bmtk.simulator import pointnet\n", + "\n", + "configure = pointnet.Config.from_json('config.built_in.json')\n", + "configure.build_env()\n", + "\n", + "network = pointnet.PointNetwork.from_config(configure)\n", + "sim = pointnet.PointSimulator.from_config(configure, network)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "48bae8e9-4b80-4c81-b67a-2bc3759e75ad", + "metadata": {}, + "source": [ + "One thing to note is that when you replace one cell-model with another you may often get widely varying results. A network that is stable or silent in using one cell type may explode when a model gets replaced. Fixing this may require the or both of the following following:\n", + "* Adjusting the **dynamics_params** of the new cell type.\n", + "* Adjust the synaptic models and parameters (especially **syn_weight**)." + ] + }, + { + "cell_type": "markdown", + "id": "2ad7f269-b7a8-474a-826d-54b18a8a9466", + "metadata": {}, + "source": [ + "## 2. Example: Importing Custom NESTML cell models
" + ] + }, + { + "cell_type": "markdown", + "id": "4aed8676-b485-4da0-8ee5-b162de8933ca", + "metadata": {}, + "source": [ + "If you aren't able to find a signficant cell model that will work for your network already built into NEST, then one option is to create it yourself (or import from an existing model) using [NESTML](https://nestml.readthedocs.io/en/latest/). NESTML is a library to build, compile, and insert customized NEST cell and synapse models into a simulation. NESTML provides a very simple language for building all kinds of cell models. And these models can be readily inserted into BMTK with only a few minor changes to the SONATA configuration files.\n", + "\n", + "#### Note\n", + "\n", + "To build cell models using NESTML it requires [installing the python package](https://nestml.readthedocs.io/en/latest/installation.html). While NESTML does have a PyPI (eg `pip`) option for installing the package, due to its rapid changes we recommend that for the time being install directly from github. Otherwise some. As of at-least version 7.0.2 (April 2024) the instructions below and in the official tutorials has issues with the PyPI install." + ] + }, + { + "cell_type": "markdown", + "id": "30752987-7dd3-4864-9177-5f69fac6f367", + "metadata": {}, + "source": [ + "### Building the models" + ] + }, + { + "cell_type": "markdown", + "id": "f3399aa0-86f6-4f7f-a1a4-5d7c17a5400d", + "metadata": {}, + "source": [ + "First step is to create and compile the NESTML cell model(s). This usually requires creating a `.nestml` file in a regular text editor and adding information about parameters, equations, inputs and outputs in a tab indented file. The documentation contains very [well written language documentation](https://nestml.readthedocs.io/en/latest/nestml_language/nestml_language_concepts.html) and their [github repo contains many example cell and synaptic models](https://github.com/nest/nestml/tree/master/models) which you can expand upon for your models. For simplicity we'll go ahead and reimplement the `Izhikevich` model from the tutorial. The model is implemented in the following:" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "1ad33736-deaa-4e8b-aaea-396fa646688a", + "metadata": {}, + "outputs": [], + "source": [ + "nestml_izh_model = \"\"\"\n", + "model custom_izh_neuron:\n", + "\n", + " state:\n", + " v mV = -65 mV # Membrane potential in mV\n", + " u real = 0 # Membrane potential recovery variable\n", + "\n", + " equations:\n", + " v' = (.04 * v * v / mV + 5 * v + (140 - u) * mV + (I_e * GOhm)) / ms\n", + " u' = a * (b * v - u * mV) / (mV * ms)\n", + "\n", + " parameters:\n", + " a real = .02 # describes time scale of recovery variable\n", + " b real = .2 # sensitivity of recovery variable\n", + " c mV = -65 mV # after-spike reset value of v\n", + " d real = 8. # after-spike reset value of u\n", + "\n", + " input:\n", + " spikes <- spike\n", + " I_e pA <- continuous\n", + "\n", + " output:\n", + " spike\n", + "\n", + " update:\n", + " integrate_odes()\n", + "\n", + " onReceive(spikes):\n", + " # add synaptic current\n", + " v += spikes * mV * s\n", + "\n", + " onCondition(v >= 30mV):\n", + " # threshold crossing\n", + " v = c\n", + " u += d\n", + " emit_spike()\n", + "\"\"\"" + ] + }, + { + "cell_type": "markdown", + "id": "0813c963-9b42-4594-9a6c-e6bb12ef3d5c", + "metadata": {}, + "source": [ + "Then we use the following to build the model" + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "id": "7c023a32-8e16-4f3f-a0a5-c06f41069df5", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "\n", + " -- N E S T --\n", + " Copyright (C) 2004 The NEST Initiative\n", + "\n", + " Version: 3.7.0\n", + " Built: May 24 2024 10:13:36\n", + "\n", + " This program is provided AS IS and comes with\n", + " NO WARRANTY. See the file LICENSE for details.\n", + "\n", + " Problems or suggestions?\n", + " Visit https://www.nest-simulator.org\n", + "\n", + " Type 'nest.help()' to find out more about NEST.\n", + "\n", + "[13,custom_izh_neuron_nestml, WARNING, [13:8;13:17]]: Variable 'a' has the same name as a physical unit!\n", + "[14,custom_izh_neuron_nestml, WARNING, [16:8;16:17]]: Variable 'd' has the same name as a physical unit!\n", + "[23,custom_izh_neuron_nestml, WARNING, [13:8;13:17]]: Variable 'a' has the same name as a physical unit!\n", + "[24,custom_izh_neuron_nestml, WARNING, [16:8;16:17]]: Variable 'd' has the same name as a physical unit!\n", + "\u001b[33mCMake Warning (dev) at CMakeLists.txt:93 (project):\n", + " cmake_minimum_required() should be called prior to this top-level project()\n", + " call. Please see the cmake-commands(7) manual for usage documentation of\n", + " both commands.\n", + "This warning is for project developers. Use -Wno-dev to suppress it.\n", + "\u001b[0m\n", + "-- The CXX compiler identification is Clang 17.0.6\n", + "-- Detecting CXX compiler ABI info\n", + "-- Detecting CXX compiler ABI info - done\n", + "-- Check for working CXX compiler: /opt/anaconda3/envs/bmtk/bin/arm64-apple-darwin20.0.0-clang++ - skipped\n", + "-- Detecting CXX compile features\n", + "-- Detecting CXX compile features - done\n", + "\u001b[0m\u001b[0m\n", + "\u001b[0m-------------------------------------------------------\u001b[0m\n", + "\u001b[0mnestml_izh_module Configuration Summary\u001b[0m\n", + "\u001b[0m-------------------------------------------------------\u001b[0m\n", + "\u001b[0m\u001b[0m\n", + "\u001b[0mC++ compiler : /opt/anaconda3/envs/bmtk/bin/arm64-apple-darwin20.0.0-clang++\u001b[0m\n", + "\u001b[0mBuild static libs : OFF\u001b[0m\n", + "\u001b[0mC++ compiler flags : -ftree-vectorize -fPIC -fstack-protector-strong -O2 -pipe -stdlib=libc++ -fvisibility-inlines-hidden -fmessage-length=0 -isystem /opt/anaconda3/envs/bmtk/include\u001b[0m\n", + "\u001b[0mNEST compiler flags : -ftree-vectorize -fPIC -fstack-protector-strong -O2 -pipe -stdlib=libc++ -fvisibility-inlines-hidden -fmessage-length=0 -isystem /opt/anaconda3/envs/bmtk/include -fdebug-prefix-map=/Users/runner/miniforge3/conda-bld/nest-simulator_1716545272563/work=/usr/local/src/conda/nest-simulator-3.7 -fdebug-prefix-map=/opt/anaconda3/envs/bmtk=/usr/local/src/conda-prefix -std=c++17 -Wall -Xclang -fopenmp -O2\u001b[0m\n", + "\u001b[0mNEST include dirs : -I/opt/anaconda3/envs/bmtk/include/nest -I/opt/anaconda3/envs/bmtk/include -I/opt/anaconda3/envs/bmtk/include -I/opt/anaconda3/envs/bmtk/include -I/opt/anaconda3/envs/bmtk/include\u001b[0m\n", + "\u001b[0mNEST libraries flags : -L/opt/anaconda3/envs/bmtk/lib/nest -lnest -lsli /opt/anaconda3/envs/bmtk/lib/libltdl.dylib /opt/anaconda3/envs/bmtk/lib/libreadline.dylib /opt/anaconda3/envs/bmtk/lib/libncurses.dylib /opt/anaconda3/envs/bmtk/lib/libgsl.dylib /opt/anaconda3/envs/bmtk/lib/libgslcblas.dylib /opt/anaconda3/envs/bmtk/lib/libomp.dylib\u001b[0m\n", + "\u001b[0m\u001b[0m\n", + "\u001b[0m-------------------------------------------------------\u001b[0m\n", + "\u001b[0m\u001b[0m\n", + "\u001b[0mYou can now build and install 'nestml_izh_module' using\u001b[0m\n", + "\u001b[0m make\u001b[0m\n", + "\u001b[0m make install\u001b[0m\n", + "\u001b[0m\u001b[0m\n", + "\u001b[0mThe library file libnestml_izh_module.so will be installed to\u001b[0m\n", + "\u001b[0m /var/folders/pr/_10c7kr546b1t7cf9z259z_40000gp/T/nestml_target_4yafvsuk\u001b[0m\n", + "\u001b[0mThe module can be loaded into NEST using\u001b[0m\n", + "\u001b[0m (nestml_izh_module) Install (in SLI)\u001b[0m\n", + "\u001b[0m nest.Install(nestml_izh_module) (in PyNEST)\u001b[0m\n", + "\u001b[0m\u001b[0m\n", + "\u001b[33mCMake Warning (dev) in CMakeLists.txt:\n", + " No cmake_minimum_required command is present. A line of code such as\n", + "\n", + " cmake_minimum_required(VERSION 3.29)\n", + "\n", + " should be added at the top of the file. The version specified may be lower\n", + " if you wish to support older CMake versions for this project. For more\n", + " information run \"cmake --help-policy CMP0000\".\n", + "This warning is for project developers. Use -Wno-dev to suppress it.\n", + "\u001b[0m\n", + "-- Configuring done (1.3s)\n", + "-- Generating done (0.0s)\n", + "-- Build files have been written to: /Users/beatriz.herrera/Documents/GitHub/bmtk/docs/tutorial/02_opt_nest_custom_models/components/nestml\n", + "[ 66%] \u001b[32mBuilding CXX object CMakeFiles/nestml_izh_module_module.dir/nestml_izh_module.o\u001b[0m\n", + "[ 66%] \u001b[32mBuilding CXX object CMakeFiles/nestml_izh_module_module.dir/custom_izh_neuron_nestml.o\u001b[0m\n", + "/Users/beatriz.herrera/Documents/GitHub/bmtk/docs/tutorial/02_opt_nest_custom_models/components/nestml/custom_izh_neuron_nestml.cpp:186:16: warning: unused variable '__timestep' [-Wunused-variable]\n", + " 186 | const double __timestep = nest::Time::get_resolution().get_ms(); // do not remove, this is necessary for the timestep() function\n", + " | ^~~~~~~~~~\n", + "/Users/beatriz.herrera/Documents/GitHub/bmtk/docs/tutorial/02_opt_nest_custom_models/components/nestml/custom_izh_neuron_nestml.cpp:262:16: warning: unused variable '__timestep' [-Wunused-variable]\n", + " 262 | const double __timestep = nest::Time::get_resolution().get_ms(); // do not remove, this is necessary for the timestep() function\n", + " | ^~~~~~~~~~\n", + "/Users/beatriz.herrera/Documents/GitHub/bmtk/docs/tutorial/02_opt_nest_custom_models/components/nestml/custom_izh_neuron_nestml.cpp:315:10: warning: unused variable 'get_t' [-Wunused-variable]\n", + " 315 | auto get_t = [origin, lag](){ return nest::Time( nest::Time::step( origin.get_steps() + lag + 1) ).get_ms(); };\n", + " | ^~~~~\n", + "/Users/beatriz.herrera/Documents/GitHub/bmtk/docs/tutorial/02_opt_nest_custom_models/components/nestml/custom_izh_neuron_nestml.cpp:309:16: warning: unused variable '__timestep' [-Wunused-variable]\n", + " 309 | const double __timestep = nest::Time::get_resolution().get_ms(); // do not remove, this is necessary for the timestep() function\n", + " | ^~~~~~~~~~\n", + "/Users/beatriz.herrera/Documents/GitHub/bmtk/docs/tutorial/02_opt_nest_custom_models/components/nestml/custom_izh_neuron_nestml.cpp:478:16: warning: unused variable '__timestep' [-Wunused-variable]\n", + " 478 | const double __timestep = nest::Time::get_resolution().get_ms(); // do not remove, this is necessary for the timestep() function \n", + " | ^~~~~~~~~~\n", + "5 warnings generated.\n", + "[100%] \u001b[32m\u001b[1mLinking CXX shared module nestml_izh_module.so\u001b[0m\n", + "[100%] Built target nestml_izh_module_module\n", + "[100%] Built target nestml_izh_module_module\n", + "\u001b[36mInstall the project...\u001b[0m\n", + "-- Install configuration: \"\"\n", + "-- Installing: /var/folders/pr/_10c7kr546b1t7cf9z259z_40000gp/T/nestml_target_4yafvsuk/nestml_izh_module.so\n", + "nestml_izh_module custom_izh_neuron_nestml\n" + ] + } + ], + "source": [ + "from pynestml.codegeneration.nest_code_generator_utils import NESTCodeGeneratorUtils\n", + "\n", + "\n", + "module_name, neuron_model_name = NESTCodeGeneratorUtils.generate_code_for(\n", + " nestml_izh_model,\n", + " module_name='nestml_izh_module',\n", + " target_path='components/nestml'\n", + ")\n", + "\n", + "print(module_name, neuron_model_name)" + ] + }, + { + "cell_type": "markdown", + "id": "8079de05-1e0f-4849-99df-3c69b68762cf", + "metadata": {}, + "source": [ + "If successful, you should see it call `cmake` and `make` commands to compile the model in nest and create a library *.so (along with source code) in the **components/nestml/** directory.\n", + "\n", + "**Note** that although in this case we passed in the model as a string into the `generate_code_for` function, you also have an option of saving the model to a *.nestml textfile and passing it in. In said case it would just be the following\n", + "\n", + "```python\n", + "module_name, neuron_model_name = NESTCodeGeneratorUtils.generate_code_for(\n", + " 'nestml_izh_model.nestml',\n", + " module_name='nestml_izh_module',\n", + " target_path='components/nestml'\n", + ")\n", + "```\n" + ] + }, + { + "cell_type": "markdown", + "id": "5f40741c-5b99-4b33-83c3-d20ab79e35a2", + "metadata": {}, + "source": [ + "### 3. Using the NESTML model in PointNet" + ] + }, + { + "cell_type": "markdown", + "id": "29ba5b9b-c083-4cf2-9d24-e185372cd419", + "metadata": {}, + "source": [ + "Once we have compiled our module (eg .so or .a file) in the `target_path` folder we can go ahead and use the models like we would with a built-in nest models. In this case we have a network pre-built (*build_network.nestml.py*) in the network_nestml/ folder. First thing we must do is make sure that our **model_template** attributes point to the correct name of the models and have corresponding parameter files.\n", + "\n", + "Note that by default NESTML append the suffix \"_nestml\" to the name of our model. " + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "id": "ae33f42b-d1a3-4f63-8c93-540e5fc1f568", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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node_type_ideimodel_typedynamics_paramspop_namemodel_template
0100epoint_neuroncustom_izh_neuron_exc.jsonLIF_excnestml:custom_izh_neuron_nestml
1101ipoint_neuroncustom_izh_neuron_inh.jsonLIF_inhnestml:custom_izh_neuron_nestml
\n", + "
" + ], + "text/plain": [ + " node_type_id ei model_type dynamics_params pop_name \\\n", + "0 100 e point_neuron custom_izh_neuron_exc.json LIF_exc \n", + "1 101 i point_neuron custom_izh_neuron_inh.json LIF_inh \n", + "\n", + " model_template \n", + "0 nestml:custom_izh_neuron_nestml \n", + "1 nestml:custom_izh_neuron_nestml " + ] + }, + "execution_count": 7, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "pd.read_csv('network_nestml/izh_network_node_types.csv', sep=' ')" + ] + }, + { + "cell_type": "markdown", + "id": "17fede69-e4f3-4cd6-8732-c88a71758116", + "metadata": {}, + "source": [ + "We must also let PointNet know where the corresponding *.so file is containing the compiled model. In our SONATA configuration file we add the an \"nest_modules\" entry to the **\"components\"**\n", + "\n", + "```json\n", + " \"components\": {\n", + " \"point_neuron_models_dir\": \"$MODELS_DIR/cell_models\",\n", + "\t \"synaptic_models_dir\": \"$MODELS_DIR/synaptic_models\",\n", + " \"nest_modules\": \"$MODELS_DIR/nestml/nestml_izh_module.so\"\n", + " },\n", + "```\n", + "\n", + "If you have multiple NEST and/or NESTML modules you can either specify \"nest_modules\" as a directory\n", + "\n", + "```json\n", + " \"nest_modules\": \"/path/to/modules/dir/\"\n", + "```\n", + "\n", + "And BMTK will try to find and load all library files in the folder. Or alternatively you can pass in a list of library binaries:\n", + "\n", + "```json\n", + " \"nest_modules\": [\"/first/path/to/module1.so\", \"/second/path/to/module2.so\", ...]\n", + "```\n", + "\n", + "And run the simulation as normal" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "id": "2479269e-ec10-4b39-8e0b-f14ed214d5ba", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,097 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,129 [INFO] Batch processing nodes for izh_network/0.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Batch processing nodes for izh_network/0.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,143 [INFO] Batch processing nodes for virt_exc/0.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Batch processing nodes for virt_exc/0.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,319 [INFO] Setting up output directory\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Setting up output directory\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,320 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,322 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,325 [INFO] Network created.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Network created.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,326 [INFO] Build virtual cell stimulations for thalamus_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Build virtual cell stimulations for thalamus_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,335 [INFO] Starting Simulation\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Starting Simulation\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,875 [INFO] Simulation finished, finalizing results.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Simulation finished, finalizing results.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,893 [INFO] Done.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Done.\n" + ] + } + ], + "source": [ + "from bmtk.simulator import pointnet\n", + "\n", + "configure = pointnet.Config.from_json('config.nestml.json')\n", + "configure.build_env()\n", + "\n", + "network = pointnet.PointNetwork.from_config(configure)\n", + "sim = pointnet.PointSimulator.from_config(configure, network)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "5232f217-7b0c-4ebd-b2d4-4d93e320f85a", + "metadata": {}, + "source": [ + "## 3. Example: Overriding the creation of cell models
" + ] + }, + { + "cell_type": "markdown", + "id": "27bfbb77-ec4e-4f9d-bb59-fd5b6889df5c", + "metadata": {}, + "source": [ + "Before BMTK starts a simulation, it will go ahead and call on NEST to create neurons for all cells using attributes like **model_type**, **model_template**, and **dynamics_params**. BMTK will initialize the creation of these cells for you and for the vast majority of simulations the user does not have to do anythin special other than specify the models and parameters in the SONATA file. \n", + "\n", + "However, on rare occassions you may find yourself requiring more grainular control of how PointNet initializes and creates the neurons before the simulation begins. For example, you may want to find or alter cell parameters at the start of a simulation. Some models may require a non-standard way of initializing them. Or you may need to attach Parrot or Relay neurons to a subset of your cells. \n", + "\n", + "When BMTK initializes cells for simualation it will normally call the function `loadNESTModel` function in [`bmtk.simulation.pointnet.default_setters.cell_models`](). This is a simple function that calls that NEST `Create` function to initialize a batch of neurons that share the same model-name and properites.\n", + "\n", + "```python\n", + "def loadNESTModel(cell, template_name, dynamics_params):\n", + " return nest.Create(template_name, cell.n_nodes, dynamics_params)\n", + "```\n", + "\n", + "If you need to override this function, BMTK provides a special [python decorator](https://peps.python.org/pep-0318/) called `@cell_model`. To use the decorator you can add the following to your *run_pointnet.py* (or in a separate python file imported before you run the simulation) as such\n", + "\n", + "```python\n", + "from bmtk.simulator.pointnet import cell_model\n", + "\n", + "@cell_model(directive='nest', model_type='point_neuron')\n", + "def loadNESTModel(cell, template_name, dynamics_params):\n", + " nest_nodes = ... # create and initialize cells\n", + " return nest_nodes\n", + "```\n", + "To explain what is happening in this function\n", + "* The `@cell_model` decorator is placed right before our user defined function in order to tell bmtk that this is a special function that will be used in the creation of \"nest\" cells\n", + " * The parameters tell BMTK that this function will be called for every group of cells with **model_template** of format `nest:...` and have **model_type** of point_neuron\n", + "* There are three parameters the BMTK will pass into this function\n", + " * `cell` is copy of cell object and can be used to access all of an cell(s) properities (can be accessed like a dictionary)\n", + " * `template` will be the name of the model (eg. izhikevich, glif_psc, hh_cond, etc)\n", + " * `dynamics_params` will be a dictionary containing the contents of the json or hdf5 **dynamics_params**.\n", + "* Inside the function we should create our cell(s), usually using the NEST [Create](https://nest-simulator.readthedocs.io/en/v3.3/ref_material/pynest_apis.html#module-nest.lib.hl_api_nodes) function.\n", + "* You must return the results of the Create function, usually of list of ids, back to bmtk.\n", + "\n", + "During the initialization process BMTK will call this custom function. It tries to do so in batches, often initializing cells with the same models and signitures in batches.\n" + ] + }, + { + "cell_type": "markdown", + "id": "f1e646fd-c529-407c-a8c6-bc8e533641a7", + "metadata": {}, + "source": [ + "
\n", + "⚠️ WARNING: Overwriting loadNESTModel can be dangerous\n", + "
\n", + "\n", + "If you're network has many different models then overwritting `loadNESTModel` to deal with only a subset of the cells can have unintended side-effects. However bmtk lets you create specific load methods for different models\n" + ] + }, + { + "cell_type": "markdown", + "id": "d12fcb57-8c57-4fb8-86f1-6c8a431e44c9", + "metadata": {}, + "source": [ + "#### Overriding Izhikeich model\n", + "\n", + "For example, let's say we want to modify and adjust parameters on-the-fly (eg. we don't want to have to rebuild the network every time) but only for the `izhikevich` models. Specially we want to add jitter to the \"d\" parameter which won't exists for other model types. To do so we add a function `loadIzhikevich` and in the `@cell_model` parameters make sure that it only applies to `nest:Izhikevich` models, so that all other model types gets created in the default manner.\n", + "\n", + "We add the following function" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "605848aa-1c59-4929-a68f-73641e1dfec9", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator.pointnet import cell_model\n", + "from bmtk.simulator.pointnet.io_tools import io\n", + "import nest\n", + "\n", + "@cell_model(directive='nest:izhikevich', model_type='point_neuron')\n", + "def loadIzhikevich(cell, template_name, dynamics_params):\n", + " nodes = nest.Create(template_name, cell.n_nodes, dynamics_params)\n", + " \n", + " d_orig = nest.GetStatus(nodes, 'd')\n", + " jitter = cell['jitter']\n", + " d_noisy = d_orig*jitter\n", + " nest.SetStatus(nodes, {'d': d_noisy})\n", + " io.log_info(f'Modifying the parameters of {cell.n_nodes} {template_name} neurons.')\n", + " return nodes" + ] + }, + { + "cell_type": "markdown", + "id": "2238dbbe-25fa-4143-808c-2b29c838f5a4", + "metadata": {}, + "source": [ + "* First we create the cells as normal using the `Create` method. Then we use the `nest.GetStatus` and `nest.SetStatus` to update the \"d\" parameters for each cell. It is sometimes possible to alter the way `Create()` method is called, but in general it is best to use [`GetStatus`](https://nest-simulator.readthedocs.io/en/v3.3/ref_material/pynest_apis.html#module-nest.lib.hl_api_info) to alter parameters\n", + "* The network has a jitter parameter which we can multiple by the default \"d\" value and is stored in the hdf5 file. We call `cell[jitter]` to get this value as an array, and can use `cell` to fetch other cell attributes.\n", + "* As an optional measure we add some logging. This is a good sanity check to make sure our funnction is being called as it should.\n", + "\n", + "After we added our custom function we can load PointNet in the normal manner:" + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "id": "0acdb9d5-cd8a-441b-a4aa-5da5f879cdb0", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,907 [INFO] Created log file\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Created log file\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,930 [INFO] Batch processing nodes for net/0.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Batch processing nodes for net/0.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,938 [INFO] Batch processing nodes for virt_exc/0.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Batch processing nodes for virt_exc/0.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,958 [INFO] Setting up output directory\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Setting up output directory\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,959 [INFO] Building cells.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Building cells.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,960 [INFO] Modifying the parameters of 10 izhikevich neurons.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Modifying the parameters of 10 izhikevich neurons.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,961 [INFO] Building recurrent connections\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Building recurrent connections\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,963 [INFO] Network created.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Network created.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,963 [INFO] Build virtual cell stimulations for thalamus_spikes\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Build virtual cell stimulations for thalamus_spikes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:51,971 [INFO] Starting Simulation\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Starting Simulation\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:52,297 [INFO] Simulation finished, finalizing results.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Simulation finished, finalizing results.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 15:11:52,316 [INFO] Done.\n" + ] + }, + { + "name": "stderr", + "output_type": "stream", + "text": [ + "INFO:Done.\n" + ] + } + ], + "source": [ + "from bmtk.simulator import pointnet\n", + "\n", + "configure = pointnet.Config.from_json('config.iz_updated.json')\n", + "configure.build_env()\n", + "\n", + "network = pointnet.PointNetwork.from_config(configure)\n", + "sim = pointnet.PointSimulator.from_config(configure, network)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "d1fb1efd-fe46-4b56-890b-c691db36aeab", + "metadata": {}, + "source": [ + "## 4. Example: Switching between synaptic (and electrical) models
" + ] + }, + { + "cell_type": "markdown", + "id": "09f7ecab-5329-4d4a-b43e-282529e70206", + "metadata": {}, + "source": [ + "Similar to cell models, PointNet also allows modelers to switch different types of synapses between simulations. Like cells, SONATA groups synaptic and electrical connections (eg edges) by type and parameters, and you can have a network where different connections have different models. " + ] + }, + { + "cell_type": "markdown", + "id": "e660528c-e393-4d02-b22f-a3d24bf1a54a", + "metadata": {}, + "source": [ + "And like with the cells, the type of edge models in a network are controlled by the SONATA **model_template** and **dynamics_params** attributes. In the previous examples we have been using the default `static_synapse`, but the NEST simulator has a variety of different [built-in synaptic model](https://nest-simulator.readthedocs.io/en/v3.3/models/index.html#synapse) that can be readily taken advantage of in BMTK. If we wanted to replace it with one of the sypases that support [short-term plasticity](https://nest-simulator.readthedocs.io/en/v3.3/models/tsodyks2_synapse.html) we would modify the build network `add_edges` call to:\n", + "```python\n", + "\n", + "net.add_edges(\n", + " ...\n", + " model_template='tsodysk2_synapse',\n", + " dynamics_params='tsodysk2.syn_exc.json'\n", + " ...\n", + ")\n", + "```\n", + "* unlike with cell, the SONATA format does not require a `nest:` schema prefix for the **model_template**\n", + "* *tsodysk2.syn_exc.json* is a .json file that is saved in the \"synaptic_models_dir\", and each synapse model will have different parameter that will need adjusted by the modeler." + ] + }, + { + "cell_type": "markdown", + "id": "3f7f919c-9d15-4927-9751-5fa0b50198f2", + "metadata": {}, + "source": [ + "Or if the modeler is unable to rebuild the network from scratch, another option for switching between model is to open the *network/\\*edge_types.csv* file in a text editor and switch the **model_template** and **dynamics_params** values for the appropiate rows:" + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "3862d4e2-e51c-4a0e-95e8-a7cf3377c754", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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" + ], + "text/plain": [ + " node_type_id model_template model_type \\\n", + "0 100 nest:izhikevich point_neuron \n", + "\n", + " dynamics_params \n", + "0 custom_model_params.izhikevich.json " + ] + }, + "execution_count": 11, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "pd.read_csv('network_stp_syns/net_node_types.csv', sep=' ')" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "52deba3e-fe57-41bc-9840-b37c53076182", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "bmtk", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.11.9" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/02_opt_nest_custom_models/build_network.built_in.py b/docs/tutorial/02_opt_nest_custom_models/build_network.built_in.py new file mode 100644 index 00000000..2d475e11 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/build_network.built_in.py @@ -0,0 +1,48 @@ +import numpy as np +from bmtk.builder import NetworkBuilder + +# nest_model = 'nest:iaf_psc_delta' +nest_model = 'nest:izhikevich' +# nest_model = 'nest:aeif_cond_alpha' +# nest_model = 'nest:glif_psc' +dynamics_params = 'custom_model_params.default.json' + + +net = NetworkBuilder('net') +net.add_nodes( + N=100, + model_type='point_neuron', + model_template=nest_model, + dynamics_params=dynamics_params +) + +net.add_edges( + source=net.nodes(), target=net.nodes(), + connection_rule=1, + syn_weight=2.0, + delay=1.5, + dynamics_params='ExcToInh.json', + model_template='static_synapse' +) + +net.build() +net.save(output_dir='network_built_in') + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=10, + model_type='virtual' +) + +virt_exc.add_edges( + target=net.nodes(), + connection_rule=lambda *_: np.random.randint(0, 10), + syn_weight=2.0, + delay=1.0, + dynamics_params='ExcToInh.json', + model_template='static_synapse' +) + +virt_exc.build() +virt_exc.save(output_dir='network_built_in') \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/build_network.iz_updated.py b/docs/tutorial/02_opt_nest_custom_models/build_network.iz_updated.py new file mode 100644 index 00000000..22e158f9 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/build_network.iz_updated.py @@ -0,0 +1,46 @@ +import numpy as np +from bmtk.builder import NetworkBuilder + +nest_model = 'nest:izhikevich' +dynamics_params = 'custom_model_params.izhikevich.json' + + +net = NetworkBuilder('net') +net.add_nodes( + N=10, + model_type='point_neuron', + model_template=nest_model, + dynamics_params=dynamics_params, + jitter=np.random.uniform(0.5, 1.5, 10) +) + +net.add_edges( + source=net.nodes(), target=net.nodes(), + connection_rule=1, + syn_weight=2.0, + delay=1.5, + dynamics_params='ExcToInh.json', + model_template='static_synapse' +) + +net.build() +net.save(output_dir='network_iz_updated') + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=10, + model_type='virtual' +) + +virt_exc.add_edges( + target=net.nodes(), + connection_rule=lambda *_: np.random.randint(0, 10), + syn_weight=2.0, + delay=1.0, + dynamics_params='ExcToInh.json', + model_template='static_synapse' +) + +virt_exc.build() +virt_exc.save(output_dir='network_iz_updated') \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/build_network.nestml.py b/docs/tutorial/02_opt_nest_custom_models/build_network.nestml.py new file mode 100644 index 00000000..05a4e06f --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/build_network.nestml.py @@ -0,0 +1,106 @@ +import numpy as np + +from bmtk.builder import NetworkBuilder +from bmtk.builder.auxi.node_params import positions_columinar + + +lif_models = { + 'LIF_exc': { + 'N': 15, + 'ei': 'e', + 'pop_name': 'LIF_exc', + 'model_type': 'point_neuron', + 'model_template': 'nestml:custom_izh_neuron_nestml', + 'dynamics_params': 'custom_izh_neuron_exc.json' + }, + 'LIF_inh': { + 'N': 5, + 'ei': 'i', + 'pop_name': 'LIF_inh', + 'model_type': 'point_neuron', + 'model_template': 'nestml:custom_izh_neuron_nestml', + 'dynamics_params': 'custom_izh_neuron_inh.json' + } +} + +input_network_model = { + 'input_network': { + 'N': 10, + 'ei': 'e', + 'pop_name': 'input_network', + 'model_type': 'virtual' + } +} + + +def random_connections(source, target, p=0.1): + sid = source['node_id'] # Get source id + tid = target['node_id'] # Get target id + + # Avoid self-connections. + if sid == tid: + return None + + return np.random.binomial(1, p) # nsyns + + +def build_cortex_network(): + cortex = NetworkBuilder('izh_network') + for model in lif_models: + params = lif_models[model].copy() + positions = positions_columinar(N=lif_models[model]['N'], center=[0, 10.0, 0], max_radius=50.0, height=200.0) + cortex.add_nodes( + x=positions[:, 0], + y=positions[:, 1], + z=positions[:, 2], + **params + ) + + cortex.add_edges( + source={'ei': 'e'}, + connection_rule=random_connections, + connection_params={'p': 0.1}, + syn_weight=2.0, + delay=1.5, + dynamics_params='ExcToInh.json', + model_template='static_synapse' + ) + + cortex.add_edges( + source={'ei': 'i'}, + connection_rule=random_connections, + connection_params={'p': 0.1}, + syn_weight=-1.5, + delay=1.5, + dynamics_params='InhToExc.json', + model_template='static_synapse' + ) + + cortex.build() + cortex.save(output_dir='network_nestml') + + return cortex + + +def build_thalamus_network(cortex): + thalamus = NetworkBuilder('virt_exc') + thalamus.add_nodes(**input_network_model['input_network']) + + thalamus.add_edges( + target=cortex.nodes(), + connection_rule=random_connections, + connection_params={'p': 0.8}, + syn_weight=8.2, + delay=1.5, + dynamics_params='ExcToExc.json', + model_template='static_synapse' + ) + thalamus.build() + thalamus.save(output_dir='network_nestml') + + return thalamus + + +if __name__ == '__main__': + cortex_net = build_cortex_network() + thalamus_net = build_thalamus_network(cortex_net) diff --git a/docs/tutorial/02_opt_nest_custom_models/build_network.stp_syns.py b/docs/tutorial/02_opt_nest_custom_models/build_network.stp_syns.py new file mode 100644 index 00000000..f0ea01c3 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/build_network.stp_syns.py @@ -0,0 +1,49 @@ +import numpy as np +from bmtk.builder import NetworkBuilder + +nest_model = 'nest:izhikevich' +dynamics_params = 'custom_model_params.izhikevich.json' + +syn_model = 'tsodyks2_synapse' +syn_dynamic_params = 'tsodyks2.syn_exc.json' + + +net = NetworkBuilder('net') +net.add_nodes( + N=10, + model_type='point_neuron', + model_template=nest_model, + dynamics_params=dynamics_params, + jitter=np.random.uniform(0.5, 1.5, 10) +) + +net.add_edges( + source=net.nodes(), target=net.nodes(), + connection_rule=1, + syn_weight=2.0, + delay=1.5, + dynamics_params=syn_dynamic_params, + model_template=syn_model +) + +net.build() +net.save(output_dir='network_stp_syns') + + +virt_exc = NetworkBuilder('virt_exc') +virt_exc.add_nodes( + N=10, + model_type='virtual' +) + +virt_exc.add_edges( + target=net.nodes(), + connection_rule=lambda *_: np.random.randint(0, 10), + syn_weight=2.0, + delay=1.0, + dynamics_params='ExcToInh.json', + model_template='static_synapse' +) + +virt_exc.build() +virt_exc.save(output_dir='network_stp_syns') \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_izh_neuron_exc.json b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_izh_neuron_exc.json new file mode 100644 index 00000000..0e0dcd23 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_izh_neuron_exc.json @@ -0,0 +1,3 @@ +{ + +} \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_izh_neuron_inh.json b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_izh_neuron_inh.json new file mode 100644 index 00000000..0e0dcd23 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_izh_neuron_inh.json @@ -0,0 +1,3 @@ +{ + +} \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_model_params.default.json b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_model_params.default.json new file mode 100644 index 00000000..0db3279e --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_model_params.default.json @@ -0,0 +1,3 @@ +{ + +} diff --git a/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_model_params.izhikevich.json b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_model_params.izhikevich.json new file mode 100644 index 00000000..3e642608 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/cell_models/custom_model_params.izhikevich.json @@ -0,0 +1,6 @@ +{ + "a": 0.02, + "b": 0.2, + "c": -65.0, + "d": 8.0 +} diff --git a/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/ExcToExc.json b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/ExcToExc.json new file mode 100644 index 00000000..2c63c085 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/ExcToExc.json @@ -0,0 +1,2 @@ +{ +} diff --git a/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/ExcToInh.json b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/ExcToInh.json new file mode 100644 index 00000000..2c63c085 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/ExcToInh.json @@ -0,0 +1,2 @@ +{ +} diff --git a/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/InhToExc.json b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/InhToExc.json new file mode 100644 index 00000000..bfd870e4 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/InhToExc.json @@ -0,0 +1,3 @@ +{ +} + diff --git a/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/InhToInh.json b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/InhToInh.json new file mode 100644 index 00000000..bfd870e4 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/InhToInh.json @@ -0,0 +1,3 @@ +{ +} + diff --git a/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/tsodyks2.syn_exc.json b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/tsodyks2.syn_exc.json new file mode 100644 index 00000000..30328c59 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/components/synaptic_models/tsodyks2.syn_exc.json @@ -0,0 +1,5 @@ +{ + "U": 0.5, + "tau_rec": 800.0, + "tau_fac": 0.0 +} diff --git a/docs/tutorial/02_opt_nest_custom_models/config.built_in.json b/docs/tutorial/02_opt_nest_custom_models/config.built_in.json new file mode 100644 index 00000000..83014f10 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/config.built_in.json @@ -0,0 +1,67 @@ +{ + "manifest": { + "$BASE_DIR": "${configdir}", + "$NETWORK_DIR": "$BASE_DIR/network_built_in", + "$MODELS_DIR": "$BASE_DIR/components", + "$OUTPUT_DIR": "$BASE_DIR/output_built_in", + "$INPUT_DIR": "$BASE_DIR/inputs" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.001, + "block_run": false, + "block_size": 1000.0 + }, + + "inputs": { + "thalamus_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$INPUT_DIR/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + + "reports": { + }, + + "output": { + "log_file": "log.txt", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "output_dir": "$OUTPUT_DIR", + "overwrite_output_dir": true, + "quiet_simulator": true + }, + + "target_simulator":"NEST", + + "components": { + "point_neuron_models_dir": "$MODELS_DIR/cell_models", + "synaptic_models_dir": "$MODELS_DIR/synaptic_models" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": "$NETWORK_DIR/virt_exc_node_types.csv" + } + ], + "edges": [ + { + "edges_file": "$NETWORK_DIR/net_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/net_net_edge_types.csv" + }, + { + "edges_file": "$NETWORK_DIR/virt_exc_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/virt_exc_net_edge_types.csv" + } + ] + } +} \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/config.iz_updated.json b/docs/tutorial/02_opt_nest_custom_models/config.iz_updated.json new file mode 100644 index 00000000..6e5da994 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/config.iz_updated.json @@ -0,0 +1,67 @@ +{ + "manifest": { + "$BASE_DIR": "${configdir}", + "$NETWORK_DIR": "$BASE_DIR/network_iz_updated", + "$MODELS_DIR": "$BASE_DIR/components", + "$OUTPUT_DIR": "$BASE_DIR/output_iz_updated", + "$INPUT_DIR": "$BASE_DIR/inputs" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.001, + "block_run": false, + "block_size": 1000.0 + }, + + "inputs": { + "thalamus_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$INPUT_DIR/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + + "reports": { + }, + + "output": { + "log_file": "log.txt", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "output_dir": "$OUTPUT_DIR", + "overwrite_output_dir": true, + "quiet_simulator": true + }, + + "target_simulator":"NEST", + + "components": { + "point_neuron_models_dir": "$MODELS_DIR/cell_models", + "synaptic_models_dir": "$MODELS_DIR/synaptic_models" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": "$NETWORK_DIR/virt_exc_node_types.csv" + } + ], + "edges": [ + { + "edges_file": "$NETWORK_DIR/net_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/net_net_edge_types.csv" + }, + { + "edges_file": "$NETWORK_DIR/virt_exc_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/virt_exc_net_edge_types.csv" + } + ] + } +} \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/config.nestml.json b/docs/tutorial/02_opt_nest_custom_models/config.nestml.json new file mode 100644 index 00000000..0e78eb0e --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/config.nestml.json @@ -0,0 +1,67 @@ +{ + "manifest": { + "$BASE_DIR": "${configdir}", + "$NETWORK_DIR": "$BASE_DIR/network_nestml", + "$MODELS_DIR": "$BASE_DIR/components", + "$OUTPUT_DIR": "$BASE_DIR/output_nestml", + "$INPUT_DIR": "$BASE_DIR/inputs" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.01, + "block_run": false, + "block_size": 1000.0 + }, + + "inputs": { + "thalamus_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$INPUT_DIR/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + + "reports": { + }, + + "output": { + "log_file": "log.txt", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "output_dir": "$OUTPUT_DIR", + "overwrite_output_dir": true, + "quiet_simulator": true + }, + + "target_simulator":"NEST", + "components": { + "point_neuron_models_dir": "$MODELS_DIR/cell_models", + "synaptic_models_dir": "$MODELS_DIR/synaptic_models", + "nest_modules": "components/nestml/" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/izh_network_nodes.h5", + "node_types_file": "$NETWORK_DIR/izh_network_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": "$NETWORK_DIR/virt_exc_node_types.csv" + } + ], + "edges": [ + { + "edges_file": "$NETWORK_DIR/izh_network_izh_network_edges.h5", + "edge_types_file": "$NETWORK_DIR/izh_network_izh_network_edge_types.csv" + }, + { + "edges_file": "$NETWORK_DIR/virt_exc_izh_network_edges.h5", + "edge_types_file": "$NETWORK_DIR/virt_exc_izh_network_edge_types.csv" + } + ] + } +} diff --git a/docs/tutorial/02_opt_nest_custom_models/config.stp_syns.json b/docs/tutorial/02_opt_nest_custom_models/config.stp_syns.json new file mode 100644 index 00000000..97441451 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/config.stp_syns.json @@ -0,0 +1,67 @@ +{ + "manifest": { + "$BASE_DIR": "${configdir}", + "$NETWORK_DIR": "$BASE_DIR/network_stp_syns", + "$MODELS_DIR": "$BASE_DIR/components", + "$OUTPUT_DIR": "$BASE_DIR/output_stp_syns", + "$INPUT_DIR": "$BASE_DIR/inputs" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.001, + "block_run": false, + "block_size": 1000.0 + }, + + "inputs": { + "thalamus_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$INPUT_DIR/virt_exc_spikes.h5", + "node_set": "virt_exc" + } + }, + + "reports": { + }, + + "output": { + "log_file": "log.txt", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "output_dir": "$OUTPUT_DIR", + "overwrite_output_dir": true, + "quiet_simulator": true + }, + + "target_simulator":"NEST", + + "components": { + "point_neuron_models_dir": "$MODELS_DIR/cell_models", + "synaptic_models_dir": "$MODELS_DIR/synaptic_models" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/virt_exc_nodes.h5", + "node_types_file": "$NETWORK_DIR/virt_exc_node_types.csv" + } + ], + "edges": [ + { + "edges_file": "$NETWORK_DIR/net_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/net_net_edge_types.csv" + }, + { + "edges_file": "$NETWORK_DIR/virt_exc_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/virt_exc_net_edge_types.csv" + } + ] + } +} \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/custom_izhikevich_neuron.nestml b/docs/tutorial/02_opt_nest_custom_models/custom_izhikevich_neuron.nestml new file mode 100644 index 00000000..c849270a --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/custom_izhikevich_neuron.nestml @@ -0,0 +1,35 @@ +model custom_izh_neuron: + + state: + v mV = -65 mV # Membrane potential in mV + u real = 0 # Membrane potential recovery variable + + equations: + v' = (.04 * v * v / mV + 5 * v + (140 - u) * mV + (I_e * GOhm)) / ms + u' = a * (b * v - u * mV) / (mV * ms) + + parameters: + a real = .02 # describes time scale of recovery variable + b real = .2 # sensitivity of recovery variable + c mV = -65 mV # after-spike reset value of v + d real = 8. # after-spike reset value of u + + input: + spikes <- spike + I_e pA <- continuous + + output: + spike + + update: + integrate_odes() + + onReceive(spikes): + # add synaptic current + v += spikes * mV * s + + onCondition(v >= 30mV): + # threshold crossing + v = c + u += d + emit_spike() \ No newline at end of file diff --git a/docs/tutorial/02_opt_nest_custom_models/generate_model.py b/docs/tutorial/02_opt_nest_custom_models/generate_model.py new file mode 100644 index 00000000..8190d3d3 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/generate_model.py @@ -0,0 +1,51 @@ +import numpy as np + + +from pynestml.codegeneration.nest_code_generator_utils import NESTCodeGeneratorUtils + + +nestml_izh_model = """ +model custom_izh_neuron: + + state: + v mV = -65 mV # Membrane potential in mV + u real = 0 # Membrane potential recovery variable + + equations: + v' = (.04 * v * v / mV + 5 * v + (140 - u) * mV + (I_e * GOhm)) / ms + u' = a * (b * v - u * mV) / (mV * ms) + + parameters: + a real = .02 # describes time scale of recovery variable + b real = .2 # sensitivity of recovery variable + c mV = -65 mV # after-spike reset value of v + d real = 8. # after-spike reset value of u + + input: + spikes <- spike + I_e pA <- continuous + + output: + spike + + update: + integrate_odes() + + onReceive(spikes): + # add synaptic current + v += spikes * mV * s + + onCondition(v >= 30mV): + # threshold crossing + v = c + u += d + emit_spike() + +""" + +# generate and build code +module_name, neuron_model_name = NESTCodeGeneratorUtils.generate_code_for( + nestml_izh_model, module_name="nestml_izh_module", target_path="components/nestml" +) + +print(module_name, neuron_model_name) diff --git a/docs/tutorial/02_opt_nest_custom_models/inputs/virt_exc_spikes.h5 b/docs/tutorial/02_opt_nest_custom_models/inputs/virt_exc_spikes.h5 new file mode 100755 index 00000000..8b605ad6 Binary files /dev/null and b/docs/tutorial/02_opt_nest_custom_models/inputs/virt_exc_spikes.h5 differ diff --git a/docs/tutorial/02_opt_nest_custom_models/run_pointnet.built_in.py b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.built_in.py new file mode 100644 index 00000000..67c9cc36 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.built_in.py @@ -0,0 +1,18 @@ +import os, sys +from bmtk.simulator import pointnet + + +def run(config_file): + configure = pointnet.Config.from_json(config_file) + configure.build_env() + + network = pointnet.PointNetwork.from_config(configure) + sim = pointnet.PointSimulator.from_config(configure, network) + sim.run() + + +if __name__ == '__main__': + if sys.argv[-1] != __file__: + run(sys.argv[-1]) + else: + run('config.built_in.json') diff --git a/docs/tutorial/02_opt_nest_custom_models/run_pointnet.iz_updated.py b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.iz_updated.py new file mode 100644 index 00000000..4852d6c4 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.iz_updated.py @@ -0,0 +1,34 @@ +import os, sys +import nest + +from bmtk.simulator import pointnet +from bmtk.simulator.pointnet import cell_model +from bmtk.simulator.pointnet.io_tools import io + + +@cell_model(directive='nest:izhikevich', model_type='point_neuron') +def loadIzhikevich(cell, template_name, dynamics_params): + nodes = nest.Create(template_name, cell.n_nodes, dynamics_params) + + d_orig = nest.GetStatus(nodes, 'd') + jitter = cell['jitter'] + d_noisy = d_orig*jitter + nest.SetStatus(nodes, {'d': d_noisy}) + io.log_info(f'Modifying the parameters of {cell.n_nodes} {template_name} neurons.') + return nodes + + +def run(config_file): + configure = pointnet.Config.from_json(config_file) + configure.build_env() + + network = pointnet.PointNetwork.from_config(configure) + sim = pointnet.PointSimulator.from_config(configure, network) + sim.run() + + +if __name__ == '__main__': + if sys.argv[-1] != __file__: + run(sys.argv[-1]) + else: + run('config.iz_updated.json') diff --git a/docs/tutorial/02_opt_nest_custom_models/run_pointnet.nestml.py b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.nestml.py new file mode 100644 index 00000000..7eb55e50 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.nestml.py @@ -0,0 +1,20 @@ +import os, sys +# import nest + +from bmtk.simulator import pointnet + + +def run(config_file): + configure = pointnet.Config.from_json(config_file) + configure.build_env() + + network = pointnet.PointNetwork.from_config(configure) + sim = pointnet.PointSimulator.from_config(configure, network) + sim.run() + + +if __name__ == '__main__': + if sys.argv[-1] != __file__: + run(sys.argv[-1]) + else: + run('config.nestml.json') diff --git a/docs/tutorial/02_opt_nest_custom_models/run_pointnet.stp_syns.py b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.stp_syns.py new file mode 100644 index 00000000..924bcfa6 --- /dev/null +++ b/docs/tutorial/02_opt_nest_custom_models/run_pointnet.stp_syns.py @@ -0,0 +1,34 @@ +import os, sys +# import nest + +from bmtk.simulator import pointnet +# from bmtk.simulator.pointnet import cell_model +# from bmtk.simulator.pointnet.io_tools import io + + +# @cell_model(directive='nest:izhikevich', model_type='point_neuron') +# def loadIzhikevich(cell, template_name, dynamics_params): +# nodes = nest.Create(template_name, cell.n_nodes, dynamics_params) + +# d_orig = nest.GetStatus(nodes, 'd') +# jitter = cell['jitter'] +# d_noisy = d_orig*jitter +# nest.SetStatus(nodes, {'d': d_noisy}) +# io.log_info(f'Modifying the parameters of {cell.n_nodes} {template_name} neurons.') +# return nodes + + +def run(config_file): + configure = pointnet.Config.from_json(config_file) + configure.build_env() + + network = pointnet.PointNetwork.from_config(configure) + sim = pointnet.PointSimulator.from_config(configure, network) + sim.run() + + +if __name__ == '__main__': + if sys.argv[-1] != __file__: + run(sys.argv[-1]) + else: + run('config.stp_syns.json') diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/Ch_advanced_spikes_input.ipynb b/docs/tutorial/03_opt_advanced_spiking_inputs/Ch_advanced_spikes_input.ipynb new file mode 100644 index 00000000..aa6cdce2 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/Ch_advanced_spikes_input.ipynb @@ -0,0 +1,740 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "b0477c1f-ba3f-48bf-9510-c95a59f49da4", + "metadata": {}, + "source": [ + "# Advanced Methods for Driving your Network with Synapic Spike-trains" + ] + }, + { + "cell_type": "markdown", + "id": "cacc6a76-0928-42dd-9257-009a3a628810", + "metadata": {}, + "source": [ + "BMTK is designed with the goal of building and simulating large-scale, realistic models of the nervous system. An important part for most of these realistic simulations is being able to reacreate the kinds of synaptic input you would expect to see in-vivo. And in order to achieve that modelers need to be able to generate the relevant spike-trains that drive the synpatic stimuli of our simulations.\n", + "\n", + "We have other tutorials that demonstrate how to generate realistic stimli using [FilterNet](). And we have also shown how to use the BMTK [SpikeTrain]() and [PoissonSpikeTrain]() class to generate input spike-trains. In these cases it requires users generate SONATA spike-train files before running their simulations. But BMTK also always different methods for users to generate and import spike-train stimuli into their simulations; including using other formats besides SONATA, importing experimental data, as-well-as more fine-grained control of how and where cells within a network recive synaptic stimuli.\n", + "\n", + "We will cover some of such methods in this tutorial.\n", + "\n", + "**Note** - scripts and files for running this tutorial can be found in the directory [03_opt_advanced_spiking_inputs/](https://github.com/AllenInstitute/bmtk/tree/develop/docs/tutorial/03_opt_advanced_spiking_inputs)" + ] + }, + { + "cell_type": "markdown", + "id": "7d5ef55a-c1ee-4173-932f-998f916672b5", + "metadata": {}, + "source": [ + "---\n", + "\n", + "## Contents\n", + "1. [Example: Spike-train inputs from a custom created csv file](#example-spikes-from-csv)\n", + "2. [Example: Dynamically generating spike-trains with cusom function](#example-spikes-from-func)\n", + "3. [Example: Incorporating in-vivo NWB spiking data into your simulation](#example-spikes-from-nwb)\n", + "4. [Example: Forcing spotonaneous synaptic activity within a network](#example-spikes-from-spont)\n", + "\n", + "---" + ] + }, + { + "cell_type": "markdown", + "id": "7e7cd5bb-39cb-4a78-99f1-0db8e4b15d19", + "metadata": {}, + "source": [ + "## 1. Example: Using spikes from CSV
" + ] + }, + { + "cell_type": "markdown", + "id": "ee0fbbe4-28eb-466d-9173-05072374f05e", + "metadata": {}, + "source": [ + "In [Chapter 2](../Ch2_single_cell/2.%20Single%20Cell.ipynb#poisson-spike-generator) we demonstrated how to generate network spikes using the BMTK's built in PoissonSpikeGenerator class, which will produce a series of spike trains using the distribution parameters/functions we give it and save it to a SONATA spikes file using to `to_sonata` method. But BMTK also allows for input spikes to be saved and loaded using a simplier space-separated csv file, which in many cases can be easier to analyze and use with other programs.\n", + "\n", + "For example in the **network_simple_spikes/** folder (built using `build_network.simple_spikes.py`) we have a set of 10 virtual nodes that are used to synaptically drive our simulation. If we wanted each one to fire at a constant 10Hz firing rate over a 5 second interval we can use `PoissonSpikeGenerator`'s `to_csv()` class to save the spikes as a csv file" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "cdaa014a-4da1-47ae-aa2c-1a71f4e7ed88", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.utils.reports.spike_trains import PoissonSpikeGenerator\n", + "\n", + "psg = PoissonSpikeGenerator()\n", + "psg.add(\n", + " node_ids='network_csv_spikes/inputs_nodes.h5', \n", + " firing_rate=10.0, \n", + " times=(0.0, 5.0),\n", + " population='inputs'\n", + ")\n", + "psg.to_csv('inputs/simple_spikes.csv')" + ] + }, + { + "cell_type": "markdown", + "id": "ff9a62f1-46e3-406b-87a3-0cdd6f02f72a", + "metadata": {}, + "source": [ + "Now we can use any text editor or csv reader (like pandas) to read in our spikes for verification and analysis" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "a85348f1-2095-43aa-a986-2988b2968433", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Number of cells in spikes-file: 10 (expected: 5)\n", + "Avg. number of spikes per cell: 51.9 (expected: 5sec x 10Hz ~ 50)\n", + "Min spike-time: 7.6425812866751235 ms\n", + "Max spike-time: 4996.1961718686 ms\n" + ] + }, + { + "data": { + "text/html": [ + "
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" + ], + "text/plain": [ + " timestamps population node_ids\n", + "0 95.713945 inputs 0\n", + "1 126.560889 inputs 0\n", + "2 229.987050 inputs 0\n", + "3 245.051621 inputs 0\n", + "4 452.794382 inputs 0" + ] + }, + "execution_count": 2, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "spikes_inputs = pd.read_csv('inputs/simple_spikes.csv', sep=' ')\n", + "\n", + "# Let's quickly check that our csv file makes sense\n", + "n_nodes = spikes_inputs['node_ids'].unique().shape[0]\n", + "n_spikes = spikes_inputs.shape[0]\n", + "\n", + "print(f'Number of cells in spikes-file: {n_nodes} (expected: 5)')\n", + "print(f'Avg. number of spikes per cell: {n_spikes/n_nodes} (expected: 5sec x 10Hz ~ 50)')\n", + "print(f'Min spike-time: {spikes_inputs[\"timestamps\"].min()} ms')\n", + "print(f'Max spike-time: {spikes_inputs[\"timestamps\"].max()} ms')\n", + "\n", + "spikes_inputs.head()" + ] + }, + { + "cell_type": "markdown", + "id": "27aca5aa-2136-4651-bbed-66981c132591", + "metadata": {}, + "source": [ + "Then when running the simulation we can do so as we previous did with SONATA spike files but with the difference is that in the **module** type for the given input must be changed from `sonata` to `csv`:\n", + "\n", + "```json\n", + " \"inputs\": {\n", + " \"csv_spikes\": {\n", + " \"input_type\": \"spikes\",\n", + " \"module\": \"csv\",\n", + " \"input_file\": \"./inputs/simple_spikes.csv\",\n", + " \"node_set\": \"inputs\"\n", + " }\n", + " }," + ] + }, + { + "cell_type": "markdown", + "id": "c1efd9ab-888c-4b52-8caf-38f4744d2ba5", + "metadata": {}, + "source": [ + "### Creating your own csv file" + ] + }, + { + "cell_type": "markdown", + "id": "655dbe88-d071-48cc-a691-2802ef66a809", + "metadata": {}, + "source": [ + "If you don't want to use the `PoissonSpikeGenerator` class you can also create your own spike-train csv file. As we can see from above, it needs to be a space-separated text file with columns **timestamps**, **population**, and **node_ids** (order doesn't matter). Each row indicates a separate spike, with the **population** + **node_ids** columns indicating the node/cell that fired, and **timestamps** (in milliseconds) indicating when it fired.\n", + "\n", + "For example, we can use the python csv writer class to create an example input file where each cell has increasing firing rate" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "e3b0e902-dd4c-4468-ac43-a97bf372d08f", + "metadata": {}, + "outputs": [], + "source": [ + "import csv\n", + "import numpy as np\n", + "\n", + "with open('inputs/custom_spikes.csv', 'w') as csvfile:\n", + " csvwriter = csv.writer(csvfile, delimiter=' ', quotechar='#')\n", + "\n", + " # write the header\n", + " csvwriter.writerow(['timestamps', 'population', 'node_ids'])\n", + " \n", + " # For node 0 we have it fire randomly at 1 Hz for 5 seconds, for node 1 at 2Hz, etc.\n", + " for node_id in range(10):\n", + " for timestamp in np.sort(np.random.uniform(0.0, 5000.0, size=(node_id+1)*5)):\n", + " csvwriter.writerow([timestamp, 'inputs', node_id])" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "019f03cf-18dc-4e88-985f-275c6ce4cb3b", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.spike_trains import plot_raster\n", + "\n", + "_ = plot_raster(spikes_file='inputs/custom_spikes.csv', with_histogram=False)" + ] + }, + { + "cell_type": "markdown", + "id": "fdf47f53-740f-44b3-b167-aec0c3117b4c", + "metadata": {}, + "source": [ + "We can now run the simulation with our custom csv input file" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "4836b12f-9f91-40d2-9031-5e1a927245ef", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-18 22:59:55,738 [INFO] Created log file\n", + "numprocs=1\n", + "NEURON mechanisms not found in ./components/mechanisms.\n", + "2024-10-18 22:59:55,860 [INFO] Building cells.\n" + ] + }, + { + "ename": "ValueError", + "evalue": "argument not a density mechanism name.", + "output_type": "error", + "traceback": [ + "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", + "\u001b[0;31mValueError\u001b[0m Traceback (most recent call last)", + "Cell \u001b[0;32mIn[5], line 9\u001b[0m\n\u001b[1;32m 6\u001b[0m conf\u001b[38;5;241m.\u001b[39mbuild_env()\n\u001b[1;32m 8\u001b[0m net \u001b[38;5;241m=\u001b[39m bionet\u001b[38;5;241m.\u001b[39mBioNetwork\u001b[38;5;241m.\u001b[39mfrom_config(conf)\n\u001b[0;32m----> 9\u001b[0m sim \u001b[38;5;241m=\u001b[39m \u001b[43mbionet\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mBioSimulator\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mfrom_config\u001b[49m\u001b[43m(\u001b[49m\u001b[43mconf\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mnetwork\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mnet\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 10\u001b[0m sim\u001b[38;5;241m.\u001b[39mrun()\n\u001b[1;32m 12\u001b[0m _ \u001b[38;5;241m=\u001b[39m plot_raster(config_file\u001b[38;5;241m=\u001b[39m\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mconfig.csv_spikes.json\u001b[39m\u001b[38;5;124m'\u001b[39m)\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/biosimulator.py:336\u001b[0m, in \u001b[0;36mBioSimulator.from_config\u001b[0;34m(cls, config, network, set_recordings)\u001b[0m\n\u001b[1;32m 333\u001b[0m \u001b[38;5;66;03m# The network must be built before initializing the simulator because\u001b[39;00m\n\u001b[1;32m 334\u001b[0m \u001b[38;5;66;03m# gap junctions must be set up before the simulation is initialized.\u001b[39;00m\n\u001b[1;32m 335\u001b[0m network\u001b[38;5;241m.\u001b[39mio\u001b[38;5;241m.\u001b[39mlog_info(\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mBuilding cells.\u001b[39m\u001b[38;5;124m'\u001b[39m)\n\u001b[0;32m--> 336\u001b[0m \u001b[43mnetwork\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mbuild_nodes\u001b[49m\u001b[43m(\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 338\u001b[0m network\u001b[38;5;241m.\u001b[39mio\u001b[38;5;241m.\u001b[39mlog_info(\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mBuilding recurrent connections\u001b[39m\u001b[38;5;124m'\u001b[39m)\n\u001b[1;32m 339\u001b[0m network\u001b[38;5;241m.\u001b[39mbuild_recurrent_edges()\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/bionetwork.py:180\u001b[0m, in \u001b[0;36mBioNetwork.build_nodes\u001b[0;34m(self)\u001b[0m\n\u001b[1;32m 178\u001b[0m \u001b[38;5;28;01mif\u001b[39;00m node_pop\u001b[38;5;241m.\u001b[39minternal_nodes_only:\n\u001b[1;32m 179\u001b[0m \u001b[38;5;28;01mfor\u001b[39;00m node \u001b[38;5;129;01min\u001b[39;00m node_pop[MPI_rank::MPI_size]:\n\u001b[0;32m--> 180\u001b[0m cell \u001b[38;5;241m=\u001b[39m \u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43m_build_cell\u001b[49m\u001b[43m(\u001b[49m\u001b[43mbionode\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mnode\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mpopulation_name\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mnode_pop\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mname\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 181\u001b[0m node_ids_map[node\u001b[38;5;241m.\u001b[39mnode_id] \u001b[38;5;241m=\u001b[39m cell\n\u001b[1;32m 182\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_rank_node_gids[cell\u001b[38;5;241m.\u001b[39mgid] \u001b[38;5;241m=\u001b[39m cell\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/bionetwork.py:163\u001b[0m, in \u001b[0;36mBioNetwork._build_cell\u001b[0;34m(self, bionode, population_name)\u001b[0m\n\u001b[1;32m 161\u001b[0m \u001b[38;5;28;01mdef\u001b[39;00m \u001b[38;5;21m_build_cell\u001b[39m(\u001b[38;5;28mself\u001b[39m, bionode, population_name):\n\u001b[1;32m 162\u001b[0m \u001b[38;5;28;01mif\u001b[39;00m bionode\u001b[38;5;241m.\u001b[39mmodel_type \u001b[38;5;129;01min\u001b[39;00m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_model_type_map:\n\u001b[0;32m--> 163\u001b[0m cell \u001b[38;5;241m=\u001b[39m \u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43m_model_type_map\u001b[49m\u001b[43m[\u001b[49m\u001b[43mbionode\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mmodel_type\u001b[49m\u001b[43m]\u001b[49m\u001b[43m(\u001b[49m\u001b[43mbionode\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mpopulation_name\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mpopulation_name\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mbionetwork\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[43m)\u001b[49m\n\u001b[1;32m 164\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_rank_nodes_by_model[bionode\u001b[38;5;241m.\u001b[39mmodel_type][cell\u001b[38;5;241m.\u001b[39mgid] \u001b[38;5;241m=\u001b[39m cell\n\u001b[1;32m 165\u001b[0m \u001b[38;5;28;01mreturn\u001b[39;00m cell\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/biocell.py:76\u001b[0m, in \u001b[0;36mBioCell.__init__\u001b[0;34m(self, node, population_name, bionetwork)\u001b[0m\n\u001b[1;32m 75\u001b[0m \u001b[38;5;28;01mdef\u001b[39;00m \u001b[38;5;21m__init__\u001b[39m(\u001b[38;5;28mself\u001b[39m, node, population_name, bionetwork):\n\u001b[0;32m---> 76\u001b[0m \u001b[38;5;28;43msuper\u001b[39;49m\u001b[43m(\u001b[49m\u001b[43mBioCell\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[43m)\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[38;5;21;43m__init__\u001b[39;49m\u001b[43m(\u001b[49m\u001b[43mnode\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mnode\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mpopulation_name\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mpopulation_name\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mnetwork\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mbionetwork\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 78\u001b[0m \u001b[38;5;66;03m# Determine number of segments and store a list of all sections.\u001b[39;00m\n\u001b[1;32m 79\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_secs \u001b[38;5;241m=\u001b[39m []\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/cell.py:54\u001b[0m, in \u001b[0;36mCell.__init__\u001b[0;34m(self, node, population_name, network)\u001b[0m\n\u001b[1;32m 51\u001b[0m pc\u001b[38;5;241m.\u001b[39mset_gid2node(\u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39mgid, MPI_RANK)\n\u001b[1;32m 53\u001b[0m \u001b[38;5;66;03m# Load the NEURON HOC object\u001b[39;00m\n\u001b[0;32m---> 54\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_hobj \u001b[38;5;241m=\u001b[39m \u001b[43mnode\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mload_cell\u001b[49m\u001b[43m(\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 56\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_edge_props \u001b[38;5;241m=\u001b[39m []\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/sonata_adaptors.py:76\u001b[0m, in \u001b[0;36mBioNode.load_cell\u001b[0;34m(self)\u001b[0m\n\u001b[1;32m 74\u001b[0m \u001b[38;5;28;01mfor\u001b[39;00m model_processing_str \u001b[38;5;129;01min\u001b[39;00m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39mmodel_processing:\n\u001b[1;32m 75\u001b[0m processing_fnc \u001b[38;5;241m=\u001b[39m nrn\u001b[38;5;241m.\u001b[39mpy_modules\u001b[38;5;241m.\u001b[39mcell_processor(model_processing_str)\n\u001b[0;32m---> 76\u001b[0m hobj \u001b[38;5;241m=\u001b[39m \u001b[43mprocessing_fnc\u001b[49m\u001b[43m(\u001b[49m\u001b[43mhobj\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mdynamics_params\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 78\u001b[0m \u001b[38;5;28;01mreturn\u001b[39;00m hobj\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/default_setters/cell_models.py:126\u001b[0m, in \u001b[0;36maibs_perisomatic\u001b[0;34m(hobj, cell, dynamics_params)\u001b[0m\n\u001b[1;32m 124\u001b[0m \u001b[38;5;28;01mif\u001b[39;00m dynamics_params \u001b[38;5;129;01mis\u001b[39;00m \u001b[38;5;129;01mnot\u001b[39;00m \u001b[38;5;28;01mNone\u001b[39;00m:\n\u001b[1;32m 125\u001b[0m fix_axon_peri(hobj)\n\u001b[0;32m--> 126\u001b[0m \u001b[43mset_params_peri\u001b[49m\u001b[43m(\u001b[49m\u001b[43mhobj\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mdynamics_params\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 128\u001b[0m \u001b[38;5;28;01mreturn\u001b[39;00m hobj\n", + "File \u001b[0;32m/opt/conda/envs/bmtk/lib/python3.9/site-packages/bmtk-1.1.1-py3.9.egg/bmtk/simulator/bionet/default_setters/cell_models.py:180\u001b[0m, in \u001b[0;36mset_params_peri\u001b[0;34m(hobj, biophys_params)\u001b[0m\n\u001b[1;32m 178\u001b[0m \u001b[38;5;28;01mfor\u001b[39;00m sec \u001b[38;5;129;01min\u001b[39;00m sections:\n\u001b[1;32m 179\u001b[0m \u001b[38;5;28;01mif\u001b[39;00m p[\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mmechanism\u001b[39m\u001b[38;5;124m\"\u001b[39m] \u001b[38;5;241m!=\u001b[39m \u001b[38;5;124m\"\u001b[39m\u001b[38;5;124m\"\u001b[39m:\n\u001b[0;32m--> 180\u001b[0m \u001b[43msec\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43minsert\u001b[49m\u001b[43m(\u001b[49m\u001b[43mp\u001b[49m\u001b[43m[\u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mmechanism\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m]\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m 181\u001b[0m \u001b[38;5;28msetattr\u001b[39m(sec, p[\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mname\u001b[39m\u001b[38;5;124m\"\u001b[39m], p[\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mvalue\u001b[39m\u001b[38;5;124m\"\u001b[39m])\n\u001b[1;32m 183\u001b[0m \u001b[38;5;66;03m# Set reversal potentials\u001b[39;00m\n", + "\u001b[0;31mValueError\u001b[0m: argument not a density mechanism name." + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "from bmtk.analyzer.spike_trains import plot_raster, to_dataframe\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.csv_spikes.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()\n", + "\n", + "_ = plot_raster(config_file='config.csv_spikes.json')" + ] + }, + { + "cell_type": "markdown", + "id": "76baf2be-28b2-4944-981b-d7e12fad5578", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 2. Example: Dynamically generating custom spike-trains " + ] + }, + { + "cell_type": "markdown", + "id": "6e1860a4-4702-4e6c-b839-5d4372acd95b", + "metadata": {}, + "source": [ + "Having to pregenerate spike-trains for you simulations inside a hdf5 or CSV file is computationally efficent and makes your results easier to reproduce and share. However, at times it may be beneificial for users to generate spike-trains dynamically during each simulation. If you are doing quick simulation and spot-checks it can be cumbersome having to regenerate a file beforehand. Or if a user is running thousand of simulations, or doing some kind of gradient search, the cost of having to create potentially thousand of spike files beforehand may not be reasonable.\n", + "\n", + "BMTK also allows modlers the option to create their own special function which will generate new spike-trains at the start of each simulation. To do so, you only need to make changes to the configuration for a \"spikes\" input so that the **module** is set to value `function`, and instead of spikes_input you specify a **spikes_function** parameter that will be the name of your custom function\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"LGN_spikes_sonata\": {\n", + " \"input_type\": \"spikes\",\n", + " \"module\": \"function\",\n", + " \"spikes_function\": \"my_spikes_generator\",\n", + " \"node_set\": \"LGN\"\n", + " }\n", + "}\n", + "```" + ] + }, + { + "cell_type": "markdown", + "id": "2a677788-8993-4612-b595-51c4bff5ba30", + "metadata": {}, + "source": [ + "For BMTK to know where to find the `my_spikes_generator` you must then use the `@spikes_generator` at the top of a given function in your run_bmtk.py script (or any python file imported into run script). In the below example we use the following to return spike trains for every cell in the `LGN` node_set to fire at a constant rate (although different rates for differnt models)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "8014e00c-b6b8-42c9-a37b-13df612ff431", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator.bionet.io_tools import io\n", + "from bmtk.simulator.bionet import spikes_generator\n", + "import numpy as np\n", + "\n", + "@spikes_generator\n", + "def my_spikes_generator(node, sim):\n", + " io.log_info(f'Generating custom spike trains for {node.node_id} from {node.population_name}')\n", + " if node['pop_name'] == 'tON':\n", + " return np.arange(100.0, sim.tstop, step=sim.dt*10)\n", + " elif node['pop_name'] == 'tOFF':\n", + " return np.arange(100.0, sim.tstop, step=sim.dt*20)\n", + " else:\n", + " return []\n" + ] + }, + { + "cell_type": "markdown", + "id": "07f07bf1-fc89-46cb-b23f-35fe33c0a041", + "metadata": {}, + "source": [ + "* All `spikes_generator` functions must have parameters `node` and `sim`\n", + " * `node` allow you to access information about each node/cell like a dictionary.\n", + " * `sim` is a class containing information about a current simulation, including information like start time (`sim.tstart`), stop_time (`sim.tstop`), step size (`sim.dt`) among other properties.\n", + "* The `spikes_generator` should return either a list or array of timestamps, in milliseconds, for each node.\n", + "* For good measure, we include logging statement. This is not required, but is good for debugging and making sure our custom function is being called.\n", + "\n", + "When the simulation is ran the `my_spikes_generator` function will be called once for each node in the specified **node_set**. " + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "36732033-c4f4-4c36-994c-b2ec5ef44c86", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.spikes_generator.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "ce4e0aca-0600-4b82-87f0-151f168e2de9", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 3. Example: Incorporating real data with NWB " + ] + }, + { + "cell_type": "markdown", + "id": "7a0a55bc-a592-4778-9f6d-0862da876721", + "metadata": {}, + "source": [ + "While you can use tools like FilterNet or PoissonSpikeGenerator to create theoretical-to-realistic synaptic stimuli onto a network from a variety of different modes. But better yet, when actual experimental data is available, it is often possible to just use that. Especially as as more-and-more experimental electrophysiological data sets are being made publically available through resources like [DANDI](https://dandiarchive.org/) or [The Allen Brain Observatory](https://portal.brain-map.org/circuits-behavior/visual-coding-neuropixels) we will want to not only use such data as a base-line for validating and comparing our models, but to actually use the data within specific simulations. For example, when modeling a network of one population and/or region, we will want use recordings of surrounding cells to help excite and inhibit our cells in a more realistic manner.\n", + "\n", + "Traditionally a major issue with using encorporating experimental ephys data into simulations is trying to parse the wide variety of different ways the data was stored. Luckily, the [Neurodata Without Borders (NWB)](https://www.nwb.org/) has developed a field-adopted format for storing experimental data. BMTK can take these files and automatically insert them into simulations.\n", + "\n", + "In this example we will take the previous model of the Mouse Primary Visual Cortex and add inputs that we know come from higher cortical regions (in this case just the VisL and Hippocampal regions) along with the input from the LGN. For these added regions, we will use actual neuropixels recordings of activity from the region during drifting gratings" + ] + }, + { + "cell_type": "markdown", + "id": "fd7df1db-fa11-45e9-ba96-f803a2e7d210", + "metadata": {}, + "source": [ + "### Step 1: Download the data" + ] + }, + { + "cell_type": "markdown", + "id": "d183688f-c1fd-477d-ad34-cb91106b0b9b", + "metadata": {}, + "source": [ + "First step is to find experimental NWB that includes spiking events. For our example we will use data from Allen Institute Visual Coding Data downloaded using the AllenSDK. We will get a three experimental sessions that we know contains recordings the the VisL and hippocamus." + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "7f79d70f-b028-4e17-8991-26f54ae130db", + "metadata": {}, + "outputs": [], + "source": [ + "from allensdk.brain_observatory.ecephys.ecephys_project_cache import EcephysProjectCache\n", + "\n", + "cache = EcephysProjectCache.from_warehouse(\n", + " manifest='./ecephys_cache_dir/neuropixels.manifest.json'\n", + ")\n", + "cache.get_session_data(715093703)\n", + "cache.get_session_data(798911424)\n", + "cache.get_session_data(754829445)" + ] + }, + { + "cell_type": "markdown", + "id": "7dc957f1-abf5-4f0e-8304-89f207db88e4", + "metadata": {}, + "source": [ + "By defaul the NWB files will be downloaded into the *ecephys_cache_dir*. Since nwb files are essentially just structured HDF5 files you can use tools like [HDFView](https://www.hdfgroup.org/downloads/hdfview/) or [h5py](https://www.h5py.org/) to read them once they have been downloaded. " + ] + }, + { + "cell_type": "markdown", + "id": "f99daefa-2859-491c-8226-2edc181e8afd", + "metadata": {}, + "source": [ + "### Step 2: Connecting VISL and Hippocampal onto our V1 cells." + ] + }, + { + "cell_type": "markdown", + "id": "1ba9ede7-b667-4038-ba9a-3f46076e8a1e", + "metadata": {}, + "source": [ + "To simulation synaptic stimulation from the VisL and Hippocampal cell recordings onto our V1 model, we will need to create a population of virtual cells representing the new cells and synapses. Unforantely the electrophyiology data doesn't include information about network geometry so it is up to the modeler to decide how to connection the experimental data into our network.\n", + "\n", + "As we did before we will separate node populations called 'VISl' and 'Hippocampus' and use the NetworkBuilder to create feedforward synaptic connections\n", + "\n", + "```python\n", + "visl = NetworkBuilder('VISl')\n", + "visl.add_nodes(\n", + " N=n_visl_units,\n", + " model_type='virtual',\n", + " ...\n", + ")\n", + "visl.add_edges(\n", + " source=visal.nodes(),\n", + " target=visp.nodes(ei='e'), \n", + " connection_rule=connection_rule_e2e,\n", + " dynamics_params='AMPA_ExcToExc.json',\n", + " model_template='Exp2Syn',\n", + " ...\n", + ")\n", + "\n", + "```\n", + "\n", + "See the *./build_network.nwb_inputs.py* for the full script that builds the SONATA network found in *./network_nwb_inputs/*" + ] + }, + { + "cell_type": "markdown", + "id": "d0d7297f-d370-44c4-aa80-eb111367c040", + "metadata": {}, + "source": [ + "### Step 3: Updating the configuration file to include NWB data." + ] + }, + { + "cell_type": "markdown", + "id": "3598b866-32db-4f70-974d-fe7d59abe2e7", + "metadata": {}, + "source": [ + "Before we can run the simulation we must update the SONATA configuration file so that the simulation:\n", + "1. Knows which .nwb files to fetch spiking data from\n", + "2. Knows how to map cells (eg. NWB units) from our experimental data to cells (eg. SONATA nodes) in our 'VISl' and 'hippocampus' populations.\n", + "3. Know which interval of the experimental data to use in our simulation.\n", + "\n", + "The most straight forward way of doing this is to add the following to our configuration file (*config.nwb_inputs.json*) in the \"inputs\" section:\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"hippo_spikes\": {\n", + " \"input_type\": \"spikes\",\n", + " \"module\": \"ecephys_probe\",\n", + " \"node_set\": \"hippocampus\",\n", + " \"input_file\": \"./ecephys_cache_dir/session_715093703/session_715093703.nwb\",\n", + " \"units\": {\n", + " \"location\": [\"CA1\", \"CA3\", \"Po\"]\n", + " }\n", + " \"mapping\": \"sample\",\n", + " \"interval\": {\n", + " \"interval_name\": \"drifting_gratings\",\n", + " \"temporal_frequency\": 4.0,\n", + " \"orientation\": 90\n", + " }\n", + "\n", + " },\n", + " \"visl_spikes\": {\n", + " \"input_type\": \"spikes\",\n", + " \"module\": \"ecephys_probe\",\n", + " \"node_set\": \"VISl\",\n", + " \"input_file\": \"./ecephys_cache_dir/session_715093703/session_715093703.nwb\",\n", + " \"mapping\": \"sample\",\n", + " \"units\": {\n", + " \"location\": \"VISl\",\n", + " }\n", + " \"interval\": {\n", + " \"interval_name\": \"drifting_gratings\",\n", + " \"temporal_frequency\": 4.0,\n", + " \"orientation\": 90\n", + " },\n", + " }\n", + "}\n", + "```\n", + "* The **input_type** and **module** will always be set to values `spikes` and `ecephys_probe`, respectively, when importing extracellular electrophysiology NWB files into your simulation.\n", + "* The **node_set** is the subset of cells in our network to use as virtual cells that are generating spikes.\n", + "* The **input_file** in the name of the nwb file(s) to use for spikes. To use data from multiple sessions just use a list of files:\n", + "```json\n", + " \"input_file\": [\n", + " \"./ecephys_cache_dir/session_715093703/session_715093703.nwb\",\n", + " \"./ecephys_cache_dir/session_798911424/session_798911424.nwb\",\n", + " \"./ecephys_cache_dir/session_754829445/session_754829445.nwb\"\n", + " ]\n", + "```\n", + "* The **units** field tell us which units from the nwb file to take their spiking data from based on either specifc keywords and/or unit-id. In the Neuropixels NWB files each unit has an field called \"location\" to determine which region the data came from, which we can use here to tell that for our model's \"hippocampus\" cells use any data coming from either the CA1, CA3 or Po regions.\n", + "* **mapping** tells how to map NWB **units** -> SONATA **node_set**. Setting the value to `sample` to do a random mapping without replacement. You can also use options `sample_with_replacment`, useful if you have more nodes in your model than units in your data. Or if you have a specific mapping from NWB unit_ids to SONATA node_ids you can use optionn `units_map` (in which case **units** will point to a csv file).\n", + "* **interval** tells simulation which interval of time to fetch spikes from. Inside the NWB file there is a stimulus table that marks the stimuli at any given epoch of time. We use this table to only get spikes recorded which a \"drifting grating\" stimuli was present with a given orientation and frequency. If you know the specific time you can also use option\n", + "```json\n", + " \"interval\": [start_time_ms, end_time_ms]\n", + "```" + ] + }, + { + "cell_type": "markdown", + "id": "ef2aadb1-2d31-4d26-8574-591229cd570c", + "metadata": {}, + "source": [ + "And finally we are ready to run our simulation" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "f77bfceb-64f0-4cd1-91a8-83aac4acfc83", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.nwb_inputs.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "448dfd35-f226-471c-98e5-929d5bc71571", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 4. Example: Forcing spotonaneous synaptic activity within a network " + ] + }, + { + "cell_type": "markdown", + "id": "5b18e4ca-aba8-4f41-ab52-8e19bfebf6c4", + "metadata": {}, + "source": [ + "So far in this tutorials we've focused on generating stimuli using synaptic stimuli coming from outside our main modeled network. In other tutorials we should different types in input to drive a simulation including current-clamps, voltage-clamps, and extracellar stimulation. While these can be generate both the kinds of stimuli we might see in the experiments and living brains, they tend to not be very grainular, especially when we want to study the secondary effects of activity within a network.\n", + "\n", + "One option that BMTK gives use for having more grainular control of internal network activity is by forcing certain synapses to spontaneously fire at pre-determined times. Not only can this give us more control of network dynamics that would be much harder to achive using current clamps or feedforward spike trains. But it also let's us isolate external activity from recurrent activity." + ] + }, + { + "cell_type": "markdown", + "id": "463087a0-380e-459a-9abb-d70d659e94b2", + "metadata": {}, + "source": [ + "In BMTK this is done by adding a new input type to the \"inputs\" section the SONATA config with **input_type** and **module** called `syn_activity`. And the minimum we must define the pre-synaptic cells that will spontaneously fire and a list of firing times:\n", + "\n", + "```json\n", + "\"syn_activity\": {\n", + " \"input_type\": \"syn_activity\",\n", + " \"module\": \"syn_activity\",\n", + " \"precell_filter\": {\n", + " \"population\": \"VISp\",\n", + " \"ei\": \"e\"\n", + " },\n", + " \"timestamps\": [500.0, 1000.0, 1500.0, 2000.0, 2500.0, 3000.0, 3500.0]\n", + "}\n", + "```\n", + "* **precell_filter** determines the synapses to sponataneously activate based on the presynaptic/source cell. In this case we tell BMTK sponataneous activity to apply on to synapses with a source-cell that has attributes `population==VISp` and `ei==e`. If you know exactly which cells you want to use you can filter by `node_id`:\n", + "```json\n", + " \"node_id\": [0, 1, 2, 3],\n", + "```\n", + "* **timestamps** is a list of timestamps, in milliseconds, to activate the neuron following startup. If you have too many timestamps to add to the json directly, you can also pass in a string path to a txt file where each line is a timestamp.\n", + "\n", + "\n", + "In the above example, all the synapses with VISp, exc pre-synaptic connections will fire at the given timestamp. For further grainular control you can all set the **postcell_filter** too for filtering out synapses based on post-synaptic cell. For example if you want spontaneous firing in exc -> inh connections (the above example would also include exc -> exc synapses:\n", + "\n", + "```json\n", + "\"syn_activity\": {\n", + " \"input_type\": \"syn_activity\",\n", + " \"module\": \"syn_activity\",\n", + " \"precell_filter\": {\n", + " \"population\": \"VISp\",\n", + " \"ei\": \"e\"\n", + " },\n", + " \"postcell_filter\": {\n", + " \"population\": \"VISp\",\n", + " \"ei\": \"i\"\n", + " },\n", + " \"timestamps\": [500.0, 1000.0, 1500.0, 2000.0, 2500.0, 3000.0, 3500.0]\n", + "}\n", + "```\n", + "\n" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "957112fc-f8ae-4841-95dd-0a0bd81be884", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.spont_syns.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "efdd66b3-53a0-479b-bbc4-af03a704028a", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3 (ipykernel)", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.9.7" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.csv_spikes.py b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.csv_spikes.py new file mode 100644 index 00000000..199fe18c --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.csv_spikes.py @@ -0,0 +1,51 @@ +# import os +import numpy as np +# import pandas as pd + +from bmtk.builder import NetworkBuilder + + + +np.random.seed(100) + +cortex = NetworkBuilder('cortex') +cortex.add_nodes( + N=10, + model_name='Pyr', + ei='e', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Scnn1a_473845048_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472363762_fit.json' +) + +cortex.build() +cortex.save(output_dir='network_csv_spikes') + + +### Stimuli into the VISp from LGN +inputs = NetworkBuilder('inputs') +inputs.add_nodes( + N=10, + model_type='virtual', + ei='e' +) +inputs.add_edges( + target=cortex.nodes(), source=inputs.nodes(), + connection_rule=lambda s, t: np.random.uniform(10, 30) if s['node_id'] == t['node_id'] else 0, + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.0041, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 10.0e20] +) +inputs.build() +inputs.save(output_dir='network_csv_spikes') + + + + + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.nwb_inputs.py b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.nwb_inputs.py new file mode 100644 index 00000000..1d6c07c5 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.nwb_inputs.py @@ -0,0 +1,178 @@ +# import os +import numpy as np +# import pandas as pd + +from bmtk.builder import NetworkBuilder +# from bmtk.builder.auxi.node_params import positions_columinar, xiter_random + + + +np.random.seed(100) + +visp = NetworkBuilder('VISp') +visp.add_nodes( + N=80, + model_name='Pyr', + ei='e', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Scnn1a_473845048_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472363762_fit.json' +) + +visp.add_nodes( + N=20, + model_name='Pvalb', + ei='i', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Pvalb_470522102_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472912177_fit.json' +) + +# exc --> exc connections +visp.add_edges( + source={'ei': 'e'}, target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + syn_weight=6.0e-05, + delay=2.0, + target_sections=['basal', 'apical'], + distance_range=[30.0, 150.0] +) + +# exc --> inh connections +visp.add_edges( + source={'ei': 'e'}, target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + syn_weight=0.0006, + delay=2.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20] +) + +# inh --> exc connections +visp.add_edges( + source={'ei': 'i'}, target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='GABA_InhToExc.json', + model_template='Exp2Syn', + syn_weight=0.002, + delay=2.0, + target_sections=['somatic', 'basal', 'apical'], + distance_range=[0.0, 50.0] +) + +# inh --> inh connections +visp.add_edges( + source={'ei': 'i'}, target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='GABA_InhToInh.json', + model_template='Exp2Syn', + syn_weight=0.00015, + delay=2.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20] +) + +visp.build() +visp.save(output_dir='network_nwb_inputs') + + +### Stimuli into the VISp from LGN +lgn = NetworkBuilder('LGN') +lgn.add_nodes( + N=100, + x=np.random.uniform(-40.0, 40.0, size=100), + y=np.random.uniform(-40.0, 40.0, size=100), + model_type='virtual', + ei='e' +) +lgn.add_edges( + target=visp.nodes(ei='e'), source=lgn.nodes(), + connection_rule=10, + # iterator='one_to_all', + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00041, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 50.0] +) +lgn.add_edges( + target=visp.nodes(ei='i'), source=lgn.nodes(), + connection_rule=10, + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00095, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 1e+20] +) +lgn.build() +lgn.save(output_dir='network_nwb_inputs') + + +visl = NetworkBuilder('VISl') +visl.add_nodes( + N=20, + model_type='virtual', + ei='e' +) +visl.add_edges( + target=visp.nodes(ei='e'), source=visl.nodes(), + connection_rule=lambda *_: np.random.uniform(0, 20), + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00041, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 1e+20] +) +visl.add_edges( + target=visp.nodes(ei='i'), source=visl.nodes(), + connection_rule=lambda *_: np.random.uniform(0, 20), + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00095, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 1e+20] +) +visl.build() +visl.save(output_dir='network_nwb_inputs') + + +hippo = NetworkBuilder('Hippocampus') +hippo.add_nodes( + N=20, + model_type='virtual', + ei='e' +) +hippo.add_edges( + target=visp.nodes(ei='e'), source=hippo.nodes(), + connection_rule=lambda *_: np.random.uniform(0, 10), + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00041, + target_sections=['basal', 'apical'], + distance_range=[50.0, 1e+20] +) +hippo.add_edges( + target=visp.nodes(ei='i'), source=hippo.nodes(), + connection_rule=lambda *_: np.random.uniform(0, 10), + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00095, + target_sections=['basal', 'apical'], + distance_range=[50.0, 1e+20] +) +hippo.build() +hippo.save(output_dir='network_nwb_inputs') diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.spikes_generator.py b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.spikes_generator.py new file mode 100644 index 00000000..677c0979 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.spikes_generator.py @@ -0,0 +1,137 @@ +import numpy as np +from bmtk.builder import NetworkBuilder + + +# import os +import numpy as np +# import pandas as pd + +from bmtk.builder import NetworkBuilder +# from bmtk.builder.auxi.node_params import positions_columinar, xiter_random + + + +np.random.seed(100) + +visp = NetworkBuilder('VISp') +visp.add_nodes( + N=80, + model_name='Pyr', + ei='e', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Scnn1a_473845048_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472363762_fit.json' +) + +visp.add_nodes( + N=20, + model_name='Pvalb', + ei='i', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Pvalb_470522102_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472912177_fit.json' +) + +# exc --> exc connections +visp.add_edges( + source={'ei': 'e'}, target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + syn_weight=6.0e-05, + delay=2.0, + target_sections=['basal', 'apical'], + distance_range=[30.0, 150.0] +) + +# exc --> inh connections +visp.add_edges( + source={'ei': 'e'}, target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + syn_weight=0.0006, + delay=2.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20] +) + +# inh --> exc connections +visp.add_edges( + source={'ei': 'i'}, target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='GABA_InhToExc.json', + model_template='Exp2Syn', + syn_weight=0.002, + delay=2.0, + target_sections=['somatic', 'basal', 'apical'], + distance_range=[0.0, 50.0] +) + +# inh --> inh connections +visp.add_edges( + source={'ei': 'i'}, target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='GABA_InhToInh.json', + model_template='Exp2Syn', + syn_weight=0.00015, + delay=2.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20] +) + +visp.build() +visp.save(output_dir='network_spikes_generator') + + +lgn = NetworkBuilder('LGN') + +lgn.add_nodes( + N=20, + model_type='virtual', + model_template='lgnmodel:tOFF_TF15', + x=np.random.uniform(0.0, 240.0, 20), + y=np.random.uniform(0.0, 120.0, 20), + spatial_size=1.0, + pop_name='tOFF', + dynamics_params='tOFF_TF15.json' +) +lgn.add_nodes( + N=20, + model_type='virtual', + model_template='lgnmodel:tON', + x=np.random.uniform(0.0, 240.0, 20), + y=np.random.uniform(0.0, 120.0, 20), + spatial_size=1.0, + pop_name='tON', + dynamics_params='tON_TF8.json' +) +lgn.add_edges( + target=visp.nodes(ei='e'), source=lgn.nodes(), + connection_rule=10, + # iterator='one_to_all', + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00041, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 50.0] +) +lgn.add_edges( + target=visp.nodes(ei='i'), source=lgn.nodes(), + connection_rule=10, + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00095, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 1e+20] +) + + +lgn.build() +lgn.save(output_dir='network_spikes_generator') \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.spont_syns.py b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.spont_syns.py new file mode 100644 index 00000000..04ec60b9 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/build_network.spont_syns.py @@ -0,0 +1,84 @@ +# import os +import numpy as np +# import pandas as pd + +from bmtk.builder import NetworkBuilder +# from bmtk.builder.auxi.node_params import positions_columinar, xiter_random + + + +np.random.seed(100) + +visp = NetworkBuilder('VISp') +visp.add_nodes( + N=80, + model_name='Pyr', + ei='e', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Scnn1a_473845048_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472363762_fit.json' +) + +visp.add_nodes( + N=20, + model_name='Pvalb', + ei='i', + model_type='biophysical', + model_processing='aibs_perisomatic', + morphology='Pvalb_470522102_m.swc', + model_template='ctdb:Biophys1.hoc', + dynamics_params='472912177_fit.json' +) + +# exc --> exc connections +visp.add_edges( + source={'ei': 'e'}, target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + syn_weight=6.0e-05, + delay=2.0, + target_sections=['basal', 'apical'], + distance_range=[30.0, 150.0] +) + +# exc --> inh connections +visp.add_edges( + source={'ei': 'e'}, target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + syn_weight=0.0006, + delay=2.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20] +) + +# inh --> exc connections +visp.add_edges( + source={'ei': 'i'}, target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='GABA_InhToExc.json', + model_template='Exp2Syn', + syn_weight=0.002, + delay=2.0, + target_sections=['somatic', 'basal', 'apical'], + distance_range=[0.0, 50.0] +) + +# inh --> inh connections +visp.add_edges( + source={'ei': 'i'}, target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(0, 10), + dynamics_params='GABA_InhToInh.json', + model_template='Exp2Syn', + syn_weight=0.00015, + delay=2.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20] +) + +visp.build() +visp.save(output_dir='network_spont_syns') diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/biophysical_neuron_templates/472363762_fit.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/biophysical_neuron_templates/472363762_fit.json new file mode 100644 index 00000000..99f0eb30 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/biophysical_neuron_templates/472363762_fit.json @@ -0,0 +1,173 @@ +{ + "passive": [ + { + "ra": 138.28, + "cm": [ + { + "section": "soma", + "cm": 1.0 + }, + { + "section": "axon", + "cm": 1.0 + }, + { + "section": "apic", + "cm": 2.12 + }, + { + "section": "dend", + "cm": 2.12 + } + ], + "e_pas": -92.49911499023438 + } + ], + "axon_morph": [ + { + "delete_axon": [ + "forsec axonal{delete_section()}", + " create axon[2]", + " axon[0]{", + " L= 30", + " diam = 1", + " nseg = 1+2*int(L/40)", + " all.append()", + " axonal.append()", + " }", + " axon[1]{", + " L= 30", + " diam = 1", + " nseg = 1+2*int(L/40)", + " all.append()", + " axonal.append()", + " }", + "", + "nSecAxonal = 2", + "connect axon(0), soma(0.5)", + "connect axon[1](0), axon[0](1) ", + "access soma" + ], + "setup_line": "create soma[1], dend[1], apic[1], axon[1]" + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 38 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -92.49911499023438 + } + ], + "genome": [ + { + "value": 0.0012021154978800002, + "section": "soma", + "name": "gbar_Im", + "mechanism": "Im" + }, + { + "value": 4.12225901169e-05, + "section": "soma", + "name": "gbar_Ih", + "mechanism": "Ih" + }, + { + "value": 0.98228995892999993, + "section": "soma", + "name": "gbar_NaTs", + "mechanism": "NaTs" + }, + { + "value": 0.000209348990528, + "section": "soma", + "name": "gbar_Nap", + "mechanism": "Nap" + }, + { + "value": 0.051758360920800002, + "section": "soma", + "name": "gbar_K_P", + "mechanism": "K_P" + }, + { + "value": 0.00073160714529799998, + "section": "soma", + "name": "gbar_K_T", + "mechanism": "K_T" + }, + { + "value": 0.00019222004878899999, + "section": "soma", + "name": "gbar_SK", + "mechanism": "SK" + }, + { + "value": 0.057264803402699994, + "section": "soma", + "name": "gbar_Kv3_1", + "mechanism": "Kv3_1" + }, + { + "value": 0.00053599731839199991, + "section": "soma", + "name": "gbar_Ca_HVA", + "mechanism": "Ca_HVA" + }, + { + "value": 0.0070061294358100008, + "section": "soma", + "name": "gbar_Ca_LVA", + "mechanism": "Ca_LVA" + }, + { + "value": 0.0012510775510599999, + "section": "soma", + "name": "gamma_CaDynamics", + "mechanism": "CaDynamics" + }, + { + "value": 717.91660042899991, + "section": "soma", + "name": "decay_CaDynamics", + "mechanism": "CaDynamics" + }, + { + "value": 5.71880766722e-06, + "section": "soma", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 0.00045738760076499994, + "section": "axon", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 3.2393273274400003e-06, + "section": "dend", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 9.5861855476200007e-05, + "section": "apic", + "name": "g_pas", + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/biophysical_neuron_templates/472912177_fit.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/biophysical_neuron_templates/472912177_fit.json new file mode 100644 index 00000000..f06b7f73 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/biophysical_neuron_templates/472912177_fit.json @@ -0,0 +1,163 @@ +{ + "passive": [ + { + "ra": 143.65, + "cm": [ + { + "section": "soma", + "cm": 2.16 + }, + { + "section": "axon", + "cm": 2.16 + }, + { + "section": "dend", + "cm": 2.16 + } + ], + "e_pas": -95.53709411621094 + } + ], + "axon_morph": [ + { + "delete_axon": [ + "forsec axonal{delete_section()}", + " create axon[2]", + " axon[0]{", + " L= 30", + " diam = 1", + " nseg = 1+2*int(L/40)", + " all.append()", + " axonal.append()", + " }", + " axon[1]{", + " L= 30", + " diam = 1", + " nseg = 1+2*int(L/40)", + " all.append()", + " axonal.append()", + " }", + "", + "nSecAxonal = 2", + "connect axon(0), soma(0.5)", + "connect axon[1](0), axon[0](1) ", + "access soma" + ], + "setup_line": "create soma[1], dend[1], apic[1], axon[1]" + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 55 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -95.53709411621094 + } + ], + "genome": [ + { + "value": 5.1162860430100002e-05, + "section": "soma", + "name": "gbar_Ih", + "mechanism": "Ih" + }, + { + "value": 0.058520185129300004, + "section": "soma", + "name": "gbar_NaV", + "mechanism": "NaV" + }, + { + "value": 0.00031192529327399998, + "section": "soma", + "name": "gbar_Kd", + "mechanism": "Kd" + }, + { + "value": 0.051060238264300006, + "section": "soma", + "name": "gbar_Kv2like", + "mechanism": "Kv2like" + }, + { + "value": 0.65076055389700005, + "section": "soma", + "name": "gbar_Kv3_1", + "mechanism": "Kv3_1" + }, + { + "value": 0.033385946416300001, + "section": "soma", + "name": "gbar_K_T", + "mechanism": "K_T" + }, + { + "value": 0.00775048605222, + "section": "soma", + "name": "gbar_Im_v2", + "mechanism": "Im_v2" + }, + { + "value": 0.0027340091995900003, + "section": "soma", + "name": "gbar_SK", + "mechanism": "SK" + }, + { + "value": 0.00056478972054199987, + "section": "soma", + "name": "gbar_Ca_HVA", + "mechanism": "Ca_HVA" + }, + { + "value": 0.0032114779487500003, + "section": "soma", + "name": "gbar_Ca_LVA", + "mechanism": "Ca_LVA" + }, + { + "value": 0.0077204433411699998, + "section": "soma", + "name": "gamma_CaDynamics", + "mechanism": "CaDynamics" + }, + { + "value": 20.300246788599999, + "section": "soma", + "name": "decay_CaDynamics", + "mechanism": "CaDynamics" + }, + { + "value": 0.00026705534351299998, + "section": "soma", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 0.00066246357111199989, + "section": "axon", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 9.8019833221899986e-06, + "section": "dend", + "name": "g_pas", + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/filter_models/tOFF_TF15.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/filter_models/tOFF_TF15.json new file mode 100644 index 00000000..4e9b43af --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/filter_models/tOFF_TF15.json @@ -0,0 +1,51 @@ +{ + "sopt_msg": [ + "After ", + "8", + "function evaluations, TNC returned:", + "Converged (|f_n-f_(n-1)| ~= 0)" + ], + "sopt_nf": 8, + "sopt_res": [ + 3.4416603571978417, + -2.1155994819051305, + 8.269733598229024, + 19.99148791096526 + ], + "init_prms": [ + 3.44215357, + -2.11509939, + 8.27421573, + 20.0 + ], + "opt_wts": [ + 3.4416603571978417, + -2.1155994819051305 + ], + "prms_bds": [ + [ + 1.0, + 10.0 + ], + [ + -10.0, + -0.05 + ], + [ + 2, + 100 + ], + [ + 2, + 200 + ] + ], + "opt_delays": [ + 0, + 0 + ], + "opt_kpeaks": [ + 8.269733598229024, + 19.99148791096526 + ] +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/filter_models/tON_TF8.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/filter_models/tON_TF8.json new file mode 100644 index 00000000..e6116ab8 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/filter_models/tON_TF8.json @@ -0,0 +1,51 @@ +{ + "sopt_msg": [ + "After ", + "20", + "function evaluations, TNC returned:", + "Converged (|f_n-f_(n-1)| ~= 0)" + ], + "sopt_nf": 20, + "sopt_res": [ + 4.21848979193672, + -2.404989833331929, + 8.524645232832142, + 23.246816713624384 + ], + "init_prms": [ + 4.222, + -2.404, + 8.545, + 23.019 + ], + "opt_wts": [ + 4.21848979193672, + -2.404989833331929 + ], + "prms_bds": [ + [ + 1.0, + 10.0 + ], + [ + -10.0, + -0.05 + ], + [ + 2, + 100 + ], + [ + 2, + 200 + ] + ], + "opt_delays": [ + 0, + 0 + ], + "opt_kpeaks": [ + 8.524645232832142, + 23.246816713624384 + ] +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/CaDynamics.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/CaDynamics.mod new file mode 100644 index 00000000..12af0659 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/CaDynamics.mod @@ -0,0 +1,40 @@ +: Dynamics that track inside calcium concentration +: modified from Destexhe et al. 1994 + +NEURON { + SUFFIX CaDynamics + USEION ca READ ica WRITE cai + RANGE decay, gamma, minCai, depth +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + FARADAY = (faraday) (coulombs) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) +} + +PARAMETER { + gamma = 0.05 : percent of free calcium (not buffered) + decay = 80 (ms) : rate of removal of calcium + depth = 0.1 (um) : depth of shell + minCai = 1e-4 (mM) +} + +ASSIGNED {ica (mA/cm2)} + +INITIAL { + cai = minCai +} + +STATE { + cai (mM) +} + +BREAKPOINT { SOLVE states METHOD cnexp } + +DERIVATIVE states { + cai' = -(10000)*(ica*gamma/(2*FARADAY*depth)) - (cai - minCai)/decay +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ca_HVA.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ca_HVA.mod new file mode 100644 index 00000000..84db2d3c --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ca_HVA.mod @@ -0,0 +1,82 @@ +: Reference: Reuveni, Friedman, Amitai, and Gutnick, J.Neurosci. 1993 + +NEURON { + SUFFIX Ca_HVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + UNITSOFF + : if((v == -27) ){ + : v = v+0.0001 + : } + :mAlpha = (0.055*(-27-v))/(exp((-27-v)/3.8) - 1) + mAlpha = 0.055 * vtrap(-27 - v, 3.8) + mBeta = (0.94*exp((-75-v)/17)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + hAlpha = (0.000457*exp((-13-v)/50)) + hBeta = (0.0065/(exp((-v-15)/28)+1)) + hInf = hAlpha/(hAlpha + hBeta) + hTau = 1/(hAlpha + hBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ca_LVA.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ca_LVA.mod new file mode 100644 index 00000000..ab151d0e --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ca_LVA.mod @@ -0,0 +1,69 @@ +: Comment: LVA ca channel. Note: mtau is an approximation from the plots +: Reference: Avery and Johnston 1996, tau from Randall 1997 +: Comment: shifted by -10 mv to correct for junction potential +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Ca_LVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1.0000/(1+ exp((v - -30.000)/-6)) + mTau = (5.0000 + 20.0000/(1+exp((v - -25.000)/5)))/qt + hInf = 1.0000/(1+ exp((v - -80.000)/6.4)) + hTau = (20.0000 + 50.0000/(1+exp((v - -40.000)/7)))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ih.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ih.mod new file mode 100644 index 00000000..73b97d84 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Ih.mod @@ -0,0 +1,71 @@ +: Reference: Kole,Hallermann,and Stuart, J. Neurosci. 2006 + +NEURON { + SUFFIX Ih + NONSPECIFIC_CURRENT ihcn + RANGE gbar, g, ihcn +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + ehcn = -45.0 (mV) +} + +ASSIGNED { + v (mV) + ihcn (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ihcn = g*(v-ehcn) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + : if(v == -154.9){ + : v = v + 0.0001 + : } + :mAlpha = 0.001*6.43*(v+154.9)/(exp((v+154.9)/11.9)-1) + mAlpha = 0.001 * 6.43 * vtrap(v + 154.9, 11.9) + mBeta = 0.001*193*exp(v/33.1) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Im.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Im.mod new file mode 100644 index 00000000..d6112d57 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Im.mod @@ -0,0 +1,62 @@ +: Reference: Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Im + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Im_v2.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Im_v2.mod new file mode 100644 index 00000000..fc219f71 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Im_v2.mod @@ -0,0 +1,59 @@ +: Based on Im model of Vervaeke et al. (2006) + +NEURON { + SUFFIX Im_v2 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-30)/10) + mAlpha = 0.007 * exp( (6 * 0.4 * (v - (-48))) / 26.12 ) + mBeta = 0.007 * exp( (-6 * (1 - 0.4) * (v - (-48))) / 26.12 ) + + mInf = mAlpha / (mAlpha + mBeta) + mTau = (15 + 1 / (mAlpha + mBeta)) / qt +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/K_P.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/K_P.mod new file mode 100644 index 00000000..0a1238f9 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/K_P.mod @@ -0,0 +1,71 @@ +: Comment: The persistent component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + + +NEURON { + SUFFIX K_P + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + tauF = 1 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mInf = 1 / (1 + exp(-(v - (-14.3 + vshift)) / 14.6)) + if (v < -50 + vshift){ + mTau = tauF * (1.25+175.03*exp(-(v - vshift) * -0.026))/qt + } else { + mTau = tauF * (1.25+13*exp(-(v - vshift) * 0.026))/qt + } + hInf = 1/(1 + exp(-(v - (-54 + vshift))/-11)) + hTau = (360+(1010+24*(v - (-55 + vshift)))*exp(-((v - (-75 + vshift))/48)^2))/qt + UNITSON +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/K_T.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/K_T.mod new file mode 100644 index 00000000..c31beaff --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/K_T.mod @@ -0,0 +1,68 @@ +: Comment: The transient component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + +NEURON { + SUFFIX K_T + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + mTauF = 1.0 + hTauF = 1.0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1/(1 + exp(-(v - (-47 + vshift)) / 29)) + mTau = (0.34 + mTauF * 0.92*exp(-((v+71-vshift)/59)^2))/qt + hInf = 1/(1 + exp(-(v+66-vshift)/-10)) + hTau = (8 + hTauF * 49*exp(-((v+73-vshift)/23)^2))/qt + UNITSON +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kd.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kd.mod new file mode 100644 index 00000000..82cbe59a --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kd.mod @@ -0,0 +1,62 @@ +: Based on Kd model of Foust et al. (2011) + + +NEURON { + SUFFIX Kd + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * h + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h' = (hInf - h) / hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-23)/10) + mInf = 1 - 1 / (1 + exp((v - (-43)) / 8)) + mTau = 1 + hInf = 1 / (1 + exp((v - (-67)) / 7.3)) + hTau = 1500 +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kv2like.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kv2like.mod new file mode 100644 index 00000000..1cbdf843 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kv2like.mod @@ -0,0 +1,86 @@ +: Kv2-like channel +: Adapted from model implemented in Keren et al. 2005 +: Adjusted parameters to be similar to guangxitoxin-sensitive current in mouse CA1 pyramids from Liu and Bean 2014 + + +NEURON { + SUFFIX Kv2like + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mAlpha + mBeta + mTau + hInf + h1Tau + h2Tau +} + +STATE { + m + h1 + h2 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * m * (0.5 * h1 + 0.5 * h2) + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h1' = (hInf - h1) / h1Tau + h2' = (hInf - h2) / h2Tau +} + +INITIAL{ + rates() + m = mInf + h1 = hInf + h2 = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mAlpha = 0.12 * vtrap( -(v - 43), 11.0) + mBeta = 0.02 * exp(-(v + 1.27) / 120) + mInf = mAlpha / (mAlpha + mBeta) + mTau = 2.5 * (1 / (qt * (mAlpha + mBeta))) + + hInf = 1/(1 + exp((v + 58) / 11)) + h1Tau = (360 + (1010 + 23.7 * (v + 54)) * exp(-((v + 75) / 48)^2)) / qt + h2Tau = (2350 + 1380 * exp(-0.011 * v) - 210 * exp(-0.03 * v)) / qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kv3_1.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kv3_1.mod new file mode 100644 index 00000000..e2446577 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Kv3_1.mod @@ -0,0 +1,54 @@ +: Comment: Kv3-like potassium current + +NEURON { + SUFFIX Kv3_1 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + mInf = 1/(1+exp(((v -(18.700 + vshift))/(-9.700)))) + mTau = 0.2*20.000/(1+exp(((v -(-46.560 + vshift))/(-44.140)))) + UNITSON +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaTa.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaTa.mod new file mode 100644 index 00000000..fcf7bd39 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaTa.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTa + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -48 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -69 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaTs.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaTs.mod new file mode 100644 index 00000000..f753e718 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaTs.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTs + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -40 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -66 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaV.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaV.mod new file mode 100644 index 00000000..a7023956 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/NaV.mod @@ -0,0 +1,186 @@ +TITLE Mouse sodium current +: Kinetics of Carter et al. (2012) +: Based on 37 degC recordings from mouse hippocampal CA1 pyramids + +NEURON { + SUFFIX NaV + USEION na READ ena WRITE ina + RANGE g, gbar +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) +} + +PARAMETER { + gbar = .015 (S/cm2) + + : kinetic parameters + Con = 0.01 (/ms) : closed -> inactivated transitions + Coff = 40 (/ms) : inactivated -> closed transitions + Oon = 8 (/ms) : open -> Ineg transition + Ooff = 0.05 (/ms) : Ineg -> open transition + alpha = 400 (/ms) + beta = 12 (/ms) + gamma = 250 (/ms) : opening + delta = 60 (/ms) : closing + + alfac = 2.51 + btfac = 5.32 + + : Vdep + x1 = 24 (mV) : Vdep of activation (alpha) + x2 = -24 (mV) : Vdep of deactivation (beta) +} + +ASSIGNED { + + : rates + f01 (/ms) + f02 (/ms) + f03 (/ms) + f04 (/ms) + f0O (/ms) + f11 (/ms) + f12 (/ms) + f13 (/ms) + f14 (/ms) + f1n (/ms) + fi1 (/ms) + fi2 (/ms) + fi3 (/ms) + fi4 (/ms) + fi5 (/ms) + fin (/ms) + + b01 (/ms) + b02 (/ms) + b03 (/ms) + b04 (/ms) + b0O (/ms) + b11 (/ms) + b12 (/ms) + b13 (/ms) + b14 (/ms) + b1n (/ms) + bi1 (/ms) + bi2 (/ms) + bi3 (/ms) + bi4 (/ms) + bi5 (/ms) + bin (/ms) + + v (mV) + ena (mV) + ina (milliamp/cm2) + g (S/cm2) + celsius (degC) +} + +STATE { + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + C5 FROM 0 TO 1 + I1 FROM 0 TO 1 + I2 FROM 0 TO 1 + I3 FROM 0 TO 1 + I4 FROM 0 TO 1 + I5 FROM 0 TO 1 + O FROM 0 TO 1 + I6 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * O + ina = g * (v - ena) +} + +INITIAL { + rates(v) + SOLVE seqinitial +} + +KINETIC activation +{ + rates(v) + ~ C1 <-> C2 (f01,b01) + ~ C2 <-> C3 (f02,b02) + ~ C3 <-> C4 (f03,b03) + ~ C4 <-> C5 (f04,b04) + ~ C5 <-> O (f0O,b0O) + ~ O <-> I6 (fin,bin) + ~ I1 <-> I2 (f11,b11) + ~ I2 <-> I3 (f12,b12) + ~ I3 <-> I4 (f13,b13) + ~ I4 <-> I5 (f14,b14) + ~ I5 <-> I6 (f1n,b1n) + ~ C1 <-> I1 (fi1,bi1) + ~ C2 <-> I2 (fi2,bi2) + ~ C3 <-> I3 (fi3,bi3) + ~ C4 <-> I4 (fi4,bi4) + ~ C5 <-> I5 (fi5,bi5) + + CONSERVE C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +LINEAR seqinitial { : sets initial equilibrium + ~ I1*bi1 + C2*b01 - C1*( fi1+f01) = 0 + ~ C1*f01 + I2*bi2 + C3*b02 - C2*(b01+fi2+f02) = 0 + ~ C2*f02 + I3*bi3 + C4*b03 - C3*(b02+fi3+f03) = 0 + ~ C3*f03 + I4*bi4 + C5*b04 - C4*(b03+fi4+f04) = 0 + ~ C4*f04 + I5*bi5 + O*b0O - C5*(b04+fi5+f0O) = 0 + ~ C5*f0O + I6*bin - O*(b0O+fin) = 0 + + ~ C1*fi1 + I2*b11 - I1*( bi1+f11) = 0 + ~ I1*f11 + C2*fi2 + I3*b12 - I2*(b11+bi2+f12) = 0 + ~ I2*f12 + C3*fi3 + I4*bi3 - I3*(b12+bi3+f13) = 0 + ~ I3*f13 + C4*fi4 + I5*b14 - I4*(b13+bi4+f14) = 0 + ~ I4*f14 + C5*fi5 + I6*b1n - I5*(b14+bi5+f1n) = 0 + + ~ C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +PROCEDURE rates(v(mV) ) +{ + LOCAL qt + qt = 2.3^((celsius-37)/10) + + f01 = qt * 4 * alpha * exp(v/x1) + f02 = qt * 3 * alpha * exp(v/x1) + f03 = qt * 2 * alpha * exp(v/x1) + f04 = qt * 1 * alpha * exp(v/x1) + f0O = qt * gamma + f11 = qt * 4 * alpha * alfac * exp(v/x1) + f12 = qt * 3 * alpha * alfac * exp(v/x1) + f13 = qt * 2 * alpha * alfac * exp(v/x1) + f14 = qt * 1 * alpha * alfac * exp(v/x1) + f1n = qt * gamma + fi1 = qt * Con + fi2 = qt * Con * alfac + fi3 = qt * Con * alfac^2 + fi4 = qt * Con * alfac^3 + fi5 = qt * Con * alfac^4 + fin = qt * Oon + + b01 = qt * 1 * beta * exp(v/x2) + b02 = qt * 2 * beta * exp(v/x2) + b03 = qt * 3 * beta * exp(v/x2) + b04 = qt * 4 * beta * exp(v/x2) + b0O = qt * delta + b11 = qt * 1 * beta * exp(v/x2) / btfac + b12 = qt * 2 * beta * exp(v/x2) / btfac + b13 = qt * 3 * beta * exp(v/x2) / btfac + b14 = qt * 4 * beta * exp(v/x2) / btfac + b1n = qt * delta + bi1 = qt * Coff + bi2 = qt * Coff / (btfac) + bi3 = qt * Coff / (btfac^2) + bi4 = qt * Coff / (btfac^3) + bi5 = qt * Coff / (btfac^4) + bin = qt * Ooff +} + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Nap.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Nap.mod new file mode 100644 index 00000000..ef8021ec --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/Nap.mod @@ -0,0 +1,77 @@ +:Reference : Modeled according to kinetics derived from Magistretti & Alonso 1999 +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Nap + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + hInf + hTau + hAlpha + hBeta +} + +STATE { + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + rates() + g = gbar*mInf*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1.0/(1+exp((v- -52.6)/-4.6)) : assuming instantaneous activation as modeled by Magistretti and Alonso + + hInf = 1.0/(1+exp((v- -48.8)/10)) + hAlpha = 2.88e-6 * vtrap(v + 17, 4.63) + hBeta = 6.94e-6 * vtrap(-(v + 64.4), 2.63) + + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/SK.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/SK.mod new file mode 100644 index 00000000..8bfa3b72 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/SK.mod @@ -0,0 +1,56 @@ +: SK-type calcium-activated potassium current +: Reference : Kohler et al. 1996 + +NEURON { + SUFFIX SK + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gbar, g, ik +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (milli/liter) +} + +PARAMETER { + v (mV) + gbar = .000001 (mho/cm2) + zTau = 1 (ms) + ek (mV) + cai (mM) +} + +ASSIGNED { + zInf + ik (mA/cm2) + g (S/cm2) +} + +STATE { + z FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * z + ik = g * (v - ek) +} + +DERIVATIVE states { + rates(cai) + z' = (zInf - z) / zTau +} + +PROCEDURE rates(ca(mM)) { + if(ca < 1e-7){ + ca = ca + 1e-07 + } + zInf = 1/(1 + (0.00043 / ca)^4.8) +} + +INITIAL { + rates(cai) + z = zInf +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/exp1isyn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/exp1isyn.mod new file mode 100644 index 00000000..e8700401 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/exp1isyn.mod @@ -0,0 +1,52 @@ +COMMENT +Current-based version of the conductance-based model exp1syn (refer to the exp1syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + +NEURON { + POINT_PROCESS exp1isyn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*e +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/exp1syn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/exp1syn.mod new file mode 100644 index 00000000..6763e687 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/exp1syn.mod @@ -0,0 +1,42 @@ +NEURON { + POINT_PROCESS exp1syn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v-e) +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp1syn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp1syn.mod new file mode 100644 index 00000000..3490c587 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp1syn.mod @@ -0,0 +1,75 @@ +COMMENT +A model (# 1 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp1syn + RANGE e, i, tau_r, p0, tau_1 + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + g +} + +INITIAL { + n=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + + +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp2syn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp2syn.mod new file mode 100644 index 00000000..ce1d1625 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp2syn.mod @@ -0,0 +1,95 @@ +COMMENT +A model (# 2 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp2syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) + (uS) = (microsiemens) + +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_1 = baseline level of release probability + tau_1 = 5 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + tau_r + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p0 +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + tau_r' = (tau_r0-tau_r)/tau_FDR +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp3syn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp3syn.mod new file mode 100644 index 00000000..31869840 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp3syn.mod @@ -0,0 +1,102 @@ +COMMENT +A model (# 3 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp3syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D + a_D = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + tau_r + D + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 + D=1 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0*D + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p0*n*D + +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp4syn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp4syn.mod new file mode 100644 index 00000000..1ba964be --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp4syn.mod @@ -0,0 +1,83 @@ +COMMENT +A model (# 4 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by +solving Eqs (3,4) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + p = release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp4syn + RANGE e, i, tau_r, p0, tau_1,tau_f + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + p + g +} + +INITIAL { + n=1 + p=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + p' = 0 +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + p = p + p0*(1-p) + +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp5isyn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp5isyn.mod new file mode 100644 index 00000000..d6b6c105 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp5isyn.mod @@ -0,0 +1,113 @@ +COMMENT +Current-based version of the conductance-based model stp5syn (refer to the stp5syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp5isyn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of pb + pbtilde = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + p + tau_r + D + pb + g +} + +INITIAL { + n=1 + pb=pbtilde + p=pb + tau_r=tau_r0 + D=1 + g=0 + +} + +BREAKPOINT { + SOLVE state METHOD cnexp +: i = g*(v - e) + i = g*e + Pmax = n*p*D + +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp5syn.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp5syn.mod new file mode 100644 index 00000000..621fe9e1 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/stp5syn.mod @@ -0,0 +1,131 @@ +COMMENT +A model (# 5 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,4,5,8,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." +This file could be used to simulate simpler models by setting to zero the parameters in the unused equations(e.g, a_D,a_i,a_f). + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + pb = baseline vesicle release probability + p = vesicle release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where w is the static synaptic weight and Pmax is the activity-dependent factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1. + +Implemented by Sergey L. Gratiy, + +ENDCOMMENT + +: Declaring parameters as RANGE allows them to change as a function of a position alogn the cell +NEURON { + POINT_PROCESS stp5syn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +: Declaration of the default values of parameters +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of p0 + pbtilde = 0.5 + +} +: Declaration of dependent and external variables that collectively are called ASSIGNED +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +: Declaration of the state variables +STATE { + n + p + tau_r + D + pb + g +} + +: Initial conditions for the state variables +INITIAL { + n=1 + p=1 + tau_r=tau_r0 + D=1 + pb=pbtilde + g=0 + +} + +: Integration method + assignment statements +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p*D +} + +: Definition of teh dynamics between the presynaptic activations +DERIVATIVE state { + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + g' = -g/tau_1 + +} + +: This block defines what happens to the state variables at the moment presynaptic activation +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/vecevent.mod b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/vecevent.mod new file mode 100644 index 00000000..503dfd2e --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/mechanisms/modfiles/vecevent.mod @@ -0,0 +1,71 @@ +: Vector stream of events + +NEURON { + ARTIFICIAL_CELL VecStim +} + +ASSIGNED { + index + etime (ms) + space +} + +INITIAL { + index = 0 + element() + if (index > 0) { + net_send(etime - t, 1) + } +} + +NET_RECEIVE (w) { + if (flag == 1) { + net_event(t) + element() + if (index > 0) { + net_send(etime - t, 1) + } + } +} + +VERBATIM +extern double* vector_vec(); +extern int vector_capacity(); +extern void* vector_arg(); +ENDVERBATIM + +PROCEDURE element() { +VERBATIM + { void* vv; int i, size; double* px; + i = (int)index; + if (i >= 0) { + vv = *((void**)(&space)); + if (vv) { + size = vector_capacity(vv); + px = vector_vec(vv); + if (i < size) { + etime = px[i]; + index += 1.; + }else{ + index = -1.; + } + }else{ + index = -1.; + } + } + } +ENDVERBATIM +} + +PROCEDURE play() { +VERBATIM + void** vv; + vv = (void**)(&space); + *vv = (void*)0; + if (ifarg(1)) { + *vv = vector_arg(1); + } +ENDVERBATIM +} + + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/morphologies/Pvalb_470522102_m.swc b/docs/tutorial/03_opt_advanced_spiking_inputs/components/morphologies/Pvalb_470522102_m.swc new file mode 100644 index 00000000..c2700822 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/morphologies/Pvalb_470522102_m.swc @@ -0,0 +1,1966 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): K:/MouseCellTypes/176847.04.02.01/176847.04.02.01_MDF.swc_sorted.swc +# id,type,x,y,z,r,pid +1 1 237.4944 233.8336 35.28 5.9212 -1 +2 3 232.5043 230.7745 35.28 0.2669 1 +3 3 231.4495 231.199 35.28 0.2796 2 +4 3 230.3513 231.5181 35.28 0.2796 3 +5 3 229.2084 231.5159 35.28 0.2542 4 +6 3 228.0793 231.3282 35.28 0.2542 5 +7 3 226.9616 231.0846 35.28 0.2669 6 +8 3 225.8176 231.0617 35.28 0.3305 7 +9 3 224.6816 230.9313 35.2803 0.4576 8 +10 3 223.2768 230.103 35.2825 0.4068 9 +11 3 222.4268 229.3377 35.2898 0.3813 10 +12 3 221.523 228.6364 35.3265 0.3051 11 +13 3 220.8629 227.775 35.6944 0.4322 12 +14 3 220.4488 226.75 35.6216 0.4322 13 +15 3 220.1548 225.6803 34.9891 0.4068 14 +16 3 219.823 224.6198 34.3207 0.3305 15 +17 3 219.6572 223.5159 33.8643 0.2542 16 +18 3 219.7075 222.4039 33.2562 0.2288 17 +19 3 219.9523 221.3102 33.0162 0.2669 18 +20 3 220.5003 220.3138 32.8628 0.3178 19 +21 3 220.9121 219.2648 32.3744 0.3432 20 +22 3 220.7828 218.1482 31.9312 0.3559 21 +23 3 220.2623 217.1312 31.9368 0.3686 22 +24 3 220.2383 215.9895 32.0306 0.4068 23 +25 3 220.7977 214.4005 32.426 0.2288 24 +26 3 220.7211 213.2908 31.92 0.2796 25 +27 3 220.53 212.1754 31.6366 0.3305 26 +28 3 220.3333 211.0783 31.2928 0.3178 27 +29 3 219.7956 210.0899 31.5381 0.2924 28 +30 3 219.2911 209.1106 31.6926 0.2796 29 +31 3 219.0497 208.0135 31.1898 0.3051 30 +32 3 218.7763 206.921 30.8129 0.3178 31 +33 3 218.464 205.8205 30.77 0.2924 32 +34 3 218.2889 204.7039 30.5665 0.2542 33 +35 3 218.2821 203.5714 30.5469 0.2669 34 +36 3 218.321 202.4342 30.8241 0.3051 35 +37 3 218.5555 201.3246 31.1539 0.3178 36 +38 3 218.6104 200.208 31.7103 0.2924 37 +39 3 218.2374 199.159 31.2561 0.2924 38 +40 3 217.7867 198.1259 30.7882 0.3178 39 +41 3 217.2685 197.1112 30.5396 0.3305 40 +42 3 216.8612 196.156 29.3952 0.3051 41 +43 3 216.5695 195.0589 29.6587 0.3305 42 +44 3 216.0856 194.0224 29.741 0.3686 43 +45 3 214.9896 193.582 29.5781 0.2034 44 +46 3 214.1545 192.8349 29.1992 0.2161 45 +47 3 213.5436 191.9152 28.5219 0.1907 46 +48 3 212.808 191.0903 27.9535 0.1652 47 +49 3 212.3847 190.2243 26.7529 0.1907 48 +50 3 212.3012 189.141 26.1708 0.2415 49 +51 3 211.8299 188.1388 25.5808 0.2924 50 +52 3 211.6045 187.1424 24.353 0.2924 51 +53 3 211.8516 186.0933 23.4917 0.2542 52 +54 3 211.7727 184.9791 24.0668 0.2415 53 +55 3 210.9628 184.2446 23.9061 0.2415 54 +56 3 210.218 183.5571 23.1552 0.2796 55 +57 3 209.8805 182.6064 21.8562 0.2924 56 +58 3 209.6472 181.5928 21.107 0.2924 57 +59 3 209.4355 180.4889 21.1991 0.2669 58 +60 3 209.0706 179.4593 20.4764 0.2161 59 +61 3 208.7606 178.3965 20.16 0.178 60 +62 3 208.232 177.5488 20.16 0.178 61 +63 3 207.3752 177.1427 19.32 0.2161 62 +64 3 206.9748 176.1005 18.9241 0.2669 63 +65 3 206.3364 175.1773 18.48 0.2796 64 +66 3 205.9452 174.1111 18.48 0.2924 65 +67 3 205.4041 173.1158 18.2104 0.2669 66 +68 3 204.9098 172.1583 17.3872 0.2542 67 +69 3 204.7657 171.0383 17.1268 0.2161 68 +70 3 204.2257 170.0808 16.8725 0.2288 69 +71 3 203.1778 169.6403 16.8 0.2542 70 +72 3 202.218 169.0477 16.5819 0.3051 71 +73 3 201.3737 168.3247 16.1622 0.3051 72 +74 3 201.344 167.2093 15.7676 0.2669 73 +75 3 200.6576 166.5664 14.84 0.2161 74 +76 3 215.9494 194.0602 30.8 0.178 44 +77 3 215.7618 193.6575 30.8 0.1907 76 +78 3 215.7584 192.5135 30.8 0.2161 77 +79 3 215.4381 191.4347 30.8 0.2288 78 +80 3 215.064 190.3547 30.8 0.2161 79 +81 3 214.8638 189.229 30.8 0.2034 80 +82 3 214.7288 188.0953 30.8 0.178 81 +83 3 214.7391 186.9513 30.8 0.1525 82 +84 3 215.0354 185.868 31.0607 0.1398 83 +85 3 215.2894 184.8304 32.0354 0.1398 84 +86 3 215.6955 183.8339 32.737 0.1652 85 +87 3 215.9929 182.7346 32.76 0.178 86 +88 3 216.2858 181.634 32.76 0.1907 87 +89 3 216.5066 180.5587 32.2216 0.1907 88 +90 3 216.8406 179.505 31.92 0.178 89 +91 3 216.9024 178.3633 31.92 0.178 90 +92 3 216.9024 177.2193 31.92 0.178 91 +93 3 216.8258 176.0833 31.9827 0.2161 92 +94 3 216.6072 174.9965 32.5234 0.2542 93 +95 3 216.5592 173.8891 32.9048 0.2924 94 +96 3 216.5592 172.7795 33.488 0.2796 95 +97 3 216.5592 171.6366 33.6 0.2542 96 +98 3 216.5592 170.4926 33.6 0.2161 97 +99 3 216.51 169.3566 33.7218 0.2034 98 +100 3 216.4448 168.2229 33.88 0.1907 99 +101 3 216.4448 167.0812 33.9674 0.2161 100 +102 3 216.4448 165.9555 34.4448 0.2415 101 +103 3 216.4448 164.8744 35.0708 0.2415 102 +104 3 216.4448 163.7991 35.84 0.1907 103 +105 3 216.4448 162.6551 35.84 0.1525 104 +106 3 216.4448 161.5111 35.84 0.1398 105 +107 3 216.454 160.3671 35.84 0.1652 106 +108 3 216.5992 159.2334 35.84 0.178 107 +109 3 216.6736 158.0962 35.8672 0.1907 108 +110 3 216.6736 156.9591 36.1334 0.178 109 +111 3 216.8086 155.838 36.4 0.1652 110 +112 3 216.9996 154.7123 36.4 0.1525 111 +113 3 217.2239 153.6037 36.7189 0.1525 112 +114 3 217.2456 152.6611 37.7877 0.1398 113 +115 3 217.3497 151.5262 37.8 0.1271 114 +116 3 217.9183 150.6305 37.8 0.1144 115 +117 3 217.9732 149.4968 37.9033 0.1144 116 +118 3 218.2924 148.4569 38.64 0.1144 117 +119 3 218.7889 147.441 38.64 0.1271 118 +120 3 218.8472 146.3016 38.7374 0.1398 119 +121 3 219.0428 145.2148 39.3775 0.1525 120 +122 3 219.4341 144.176 39.6724 0.1398 121 +123 3 220.0427 143.2139 39.76 0.1271 122 +124 3 220.8538 142.4635 39.76 0.1144 123 +125 3 221.5081 141.6592 40.04 0.1144 124 +126 3 221.8182 140.569 40.04 0.1144 125 +127 3 222.2254 139.552 40.2016 0.1144 126 +128 3 222.4325 138.4595 40.6 0.1144 127 +129 3 222.7368 137.4161 40.6 0.1144 128 +130 3 222.7665 136.2744 40.6428 0.1144 129 +131 3 222.8706 135.1945 41.4442 0.1144 130 +132 3 223.0731 134.1271 42.0 0.1144 131 +133 3 223.2367 133.038 42.1842 0.1144 132 +134 3 223.4678 131.941 42.28 0.1144 133 +135 3 223.8808 130.9319 42.3525 0.1144 134 +136 3 224.3487 130.0717 43.12 0.1144 135 +137 3 224.8887 129.0787 43.2149 0.1144 136 +138 3 225.5041 128.239 44.3254 0.1144 137 +139 3 226.3141 127.6304 44.8 0.1144 138 +140 3 226.4376 126.5104 44.8 0.1144 139 +141 3 226.9021 125.6455 44.0115 0.1144 140 +142 3 227.3597 124.7383 43.4 0.1144 141 +143 3 227.7658 123.6893 43.4 0.1144 142 +144 3 227.8848 122.5682 43.12 0.1144 143 +145 3 228.3504 121.7079 42.5508 0.1144 144 +146 3 228.4911 120.6268 42.28 0.1144 145 +147 3 228.7931 119.5263 42.28 0.1144 146 +148 3 229.4441 118.722 42.9234 0.1144 147 +149 3 229.5711 117.6238 43.1978 0.1144 148 +150 3 229.7152 116.5324 43.68 0.1144 149 +151 3 229.706 115.4056 43.2849 0.1144 150 +152 3 229.4898 114.2952 43.12 0.1144 151 +153 3 229.4864 113.1517 43.12 0.1144 152 +154 3 229.4864 112.071 42.4463 0.1271 153 +155 3 229.4864 110.9306 42.28 0.1398 154 +156 3 229.4864 109.7952 42.1574 0.1652 155 +157 3 229.3434 108.8181 40.8668 0.1652 156 +158 3 229.1993 107.8714 39.4064 0.1652 157 +159 3 229.1432 106.7387 39.2 0.1525 158 +160 3 229.1398 105.5947 39.2 0.1525 159 +161 3 229.0288 104.4855 38.787 0.178 160 +162 3 229.0288 103.3659 38.36 0.2161 161 +163 3 228.8206 102.2481 38.36 0.2542 162 +164 3 228.6993 101.1362 38.0685 0.2415 163 +165 3 228.9144 100.2135 36.533 0.1907 164 +166 3 228.959 99.2882 35.0347 0.1398 165 +167 3 229.6809 98.7205 33.88 0.1144 166 +168 3 230.2872 97.835 33.88 0.1144 167 +169 3 230.81 96.8919 33.88 0.1144 168 +170 3 231.7824 96.2999 33.88 0.1144 169 +171 3 232.6416 95.581 33.88 0.1144 170 +172 3 233.0946 94.7028 33.88 0.1144 171 +173 3 233.7489 93.8609 33.88 0.1144 172 +174 3 234.52 93.3316 33.88 0.1271 173 +175 3 234.7545 92.236 33.88 0.1398 174 +176 3 235.5439 91.411 33.88 0.1525 175 +177 3 236.4648 90.971 34.384 0.1398 176 +178 3 236.5872 89.8573 34.44 0.1271 177 +179 3 237.4017 89.2013 33.8918 0.1144 178 +180 3 238.4348 89.1176 32.9459 0.1144 179 +181 3 239.1406 88.8369 31.351 0.1144 180 +182 3 239.5536 87.9258 30.2865 0.1144 181 +183 3 240.3361 87.2805 30.0434 0.1144 182 +184 3 241.0671 86.6914 29.12 0.1144 183 +185 3 241.8816 86.0381 28.84 0.1144 184 +186 3 243.005 85.8469 28.84 0.1144 185 +187 3 244.0644 85.4372 28.84 0.1144 186 +188 3 244.3332 84.347 28.8691 0.1144 187 +189 3 244.7531 83.4076 29.3272 0.1271 188 +190 3 245.8456 83.1688 29.4 0.1652 189 +191 3 218.8655 216.0352 29.6892 0.2288 24 +192 3 217.9709 215.4472 29.1472 0.2924 191 +193 3 217.3886 214.532 28.2688 0.3305 192 +194 3 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b/docs/tutorial/03_opt_advanced_spiking_inputs/components/morphologies/Scnn1a_473845048_m.swc new file mode 100644 index 00000000..84e566f9 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/morphologies/Scnn1a_473845048_m.swc @@ -0,0 +1,3786 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): K:/t301/production data/177300.01.02.01/reconstruction/Scnn1a-Tg3-Cre_Ai14_IVSCC_-177300.01.02.01___DS.swc +# id,type,x,y,z,r,pid +1 1 303.16 379.4648 28.56 5.4428 -1 +2 3 302.6646 375.232 23.2562 0.2524 1 +3 3 302.469 374.2356 21.9996 0.2615 2 +4 3 302.0057 373.2918 21.0081 0.3026 3 +5 3 301.2358 372.4956 20.7318 0.36 4 +6 3 300.3366 371.832 20.7847 0.3728 5 +7 3 299.7177 370.8768 20.9602 0.3575 6 +8 3 299.5049 369.798 21.7249 0.3935 7 +9 3 299.5198 368.6563 21.8372 0.4067 8 +10 3 299.6319 367.518 21.8288 0.432 9 +11 3 299.9305 366.414 21.7669 0.3941 10 +12 3 299.9534 365.2803 21.3934 0.3432 11 +13 3 299.2818 364.1844 21.835 0.3426 12 +14 3 298.4113 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4 282.3609 341.5046 39.6357 0.3792 1693 +1695 4 281.9056 340.4624 39.6536 0.3612 1694 +1696 4 281.5373 339.4008 39.2008 0.3559 1695 +1697 4 281.0682 338.378 38.8234 0.3636 1696 +1698 4 280.4699 337.4171 39.0328 0.3764 1697 +1699 4 279.8247 336.4939 39.5248 0.3734 1698 +1700 4 279.1943 335.5489 39.7446 0.3607 1699 +1701 4 278.7859 334.4942 39.7603 0.3479 1700 +1702 4 278.2929 333.4771 39.7625 0.3512 1701 +1703 4 277.7197 332.4876 39.7743 0.364 1702 +1704 4 277.1443 331.4992 39.8362 0.3848 1703 +1705 4 276.6535 330.4844 40.2108 0.394 1704 +1706 4 276.2188 329.4468 40.7154 0.4025 1705 +1707 4 275.7921 328.3909 40.88 0.3814 1706 +1708 4 275.4134 327.3133 40.8806 0.3346 1707 +1709 4 275.132 326.2047 40.8836 0.2579 1708 +1710 4 275.0668 325.0699 40.9055 0.2029 1709 +1711 4 274.6664 324.0426 41.0228 0.1995 1710 +1712 4 273.9697 323.2418 41.8933 0.2402 1711 +1713 4 273.4377 322.314 42.7798 0.2954 1712 +1714 4 273.0614 321.2993 42.1565 0.3375 1713 +1715 4 272.6152 320.7524 41.9695 0.2288 1714 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1758 +1760 4 272.7662 286.7596 38.92 0.2034 1759 +1761 4 273.0488 286.1064 40.0319 0.1923 1760 +1762 4 272.8818 285.1271 40.3096 0.2076 1761 +1763 4 272.9584 284.2691 39.5111 0.1652 1762 +1764 4 273.5498 283.6811 40.6347 0.1144 1763 +1765 4 274.1333 282.9146 39.6525 0.135 1764 +1766 4 274.2477 281.8381 39.0757 0.2477 1765 +1767 4 274.2385 280.9481 38.3975 0.2257 1766 +1768 4 273.8381 279.9242 38.4 0.1608 1767 +1769 4 273.3542 278.9278 38.1562 0.1745 1768 +1770 4 272.8348 278.0824 38.2018 0.1922 1769 +1771 4 272.3864 277.1775 38.519 0.2669 1770 +1772 4 272.1553 276.2691 37.8008 0.1528 1771 +1773 4 271.9288 275.1984 38.4185 0.1891 1772 +1774 4 272.3086 274.4124 38.0405 0.2547 1773 +1775 4 272.5752 273.4354 38.3214 0.2034 1774 +1776 4 272.3281 272.7582 38.0467 0.2834 1775 +1777 4 271.5982 272.2411 36.9163 0.2465 1776 +1778 4 271.1303 271.5684 38.2782 0.1144 1777 +1779 4 271.6863 270.8077 37.8616 0.2034 1778 +1780 4 271.875 269.9565 36.8329 0.2258 1779 +1781 4 271.6314 269.0722 37.3332 0.1783 1780 +1782 4 271.2413 268.3206 36.2782 0.2715 1781 +1783 4 271.2104 267.5198 36.0186 0.1965 1782 +1784 4 271.128 266.7373 36.9883 0.2924 1783 +1785 4 270.9244 265.726 36.4 0.2034 1784 +1786 4 270.8992 265.8656 37.24 0.2288 1785 +1787 4 271.1166 265.1277 36.12 0.1314 1785 +1788 4 271.2378 264.2434 36.2631 0.1144 1787 +1789 4 271.1177 263.1715 36.0511 0.1252 1788 +1790 4 270.9793 262.2403 37.1862 0.1181 1789 +1791 4 271.3225 261.2484 37.2089 0.1631 1790 +1792 4 271.2424 260.5483 36.1147 0.17 1791 +1793 4 271.128 259.8768 35.3027 0.2322 1792 +1794 4 271.1841 258.9398 36.1043 0.1841 1793 +1795 4 271.4712 257.9926 35.8907 0.1635 1794 +1796 4 271.5856 257.0682 35.5264 0.1904 1795 +1797 4 271.3831 256.065 34.7922 0.1447 1796 +1798 4 271.1406 254.9987 34.7516 0.2287 1797 +1799 4 271.128 254.0572 35.4824 0.2669 1798 +1800 4 271.0216 253.4772 34.2278 0.1983 1799 +1801 4 271.0948 252.5517 34.9602 0.1636 1800 +1802 4 271.3568 251.6811 35.3447 0.178 1801 +1803 4 271.8007 250.7545 35.2814 0.216 1802 +1804 4 271.9528 249.9285 34.2157 0.1446 1803 +1805 4 272.0306 249.0728 33.32 0.2889 1804 +1806 4 272.5408 248.5855 35.0 0.2669 1805 +1807 4 272.7296 247.5856 34.1986 0.1677 1806 +1808 4 272.518 246.564 34.7589 0.2193 1807 +1809 4 272.1233 245.4978 35.0 0.269 1808 +1810 4 271.8144 244.4476 35.0297 0.2444 1809 +1811 4 271.7023 243.338 35.3186 0.1659 1810 +1812 4 271.8888 242.5291 35.0 0.247 1811 +1813 4 272.5008 241.8313 35.5236 0.2798 1812 +1814 4 273.2558 241.1815 35.6112 0.2288 1813 +1815 4 273.8278 240.2755 36.2239 0.202 1814 +1816 4 274.1264 239.2516 36.0335 0.1725 1815 +1817 4 274.3266 238.1819 36.2085 0.1144 1816 +1818 4 274.6469 237.2564 35.1226 0.1695 1817 +1819 4 275.1514 236.4614 35.7316 0.1652 1818 +1820 4 275.704 235.7212 35.5776 0.1955 1819 +1821 4 275.7646 234.6676 35.6908 0.1967 1820 +1822 4 276.1353 233.7489 36.0923 0.2556 1821 +1823 4 276.276 232.7903 35.2531 0.2288 1822 +1824 4 276.7004 231.9666 34.5369 0.3238 1823 +1825 4 276.7462 230.9644 33.6 0.2204 1824 +1826 4 276.9933 230.2346 33.8254 0.2288 1825 +1827 4 277.857 229.7072 34.16 0.1144 1826 +1828 4 277.9989 228.6215 33.8125 0.2052 1827 +1829 4 278.6956 227.8299 33.6129 0.1306 1828 +1830 4 279.3431 227.1023 33.0711 0.1512 1829 +1831 4 279.2001 226.2066 33.8484 0.2924 1830 +1832 4 279.4792 225.8256 35.0 0.2415 1831 +1833 4 264.6175 308.5723 44.3792 0.3432 1729 +1834 4 264.0352 308.1101 43.7251 0.3051 1833 +1835 4 263.4106 307.3013 43.7478 0.4046 1834 +1836 4 263.0548 306.4787 43.4862 0.2063 1835 +1837 4 262.397 305.8793 43.9267 0.1913 1836 +1838 4 261.8593 305.4263 44.24 0.3159 1837 +1839 4 261.2919 304.5145 43.7203 0.405 1838 +1840 4 261.0288 303.5158 43.5868 0.3016 1839 +1841 4 261.0768 302.5445 43.787 0.2613 1840 +1842 4 260.7874 301.8112 42.4673 0.3777 1841 +1843 4 260.705 300.8548 41.2639 0.243 1842 +1844 4 260.2474 300.0071 41.8135 0.3305 1843 +1845 4 259.8985 299.3619 43.3874 0.3813 1844 +1846 4 259.1286 298.624 42.8565 0.2601 1845 +1847 4 258.655 298.0578 43.2172 0.2669 1846 +1848 4 258.1413 297.1712 43.1343 0.3247 1847 +1849 4 257.6288 296.6392 43.96 0.3686 1848 +1850 4 257.0648 295.7469 44.8678 0.276 1849 +1851 4 256.7239 294.8706 46.3028 0.1907 1850 +1852 4 256.2205 294.4965 45.6817 0.3671 1851 +1853 4 255.8956 293.5252 45.9388 0.3934 1852 +1854 4 255.7881 292.4098 45.4096 0.3386 1853 +1855 4 255.1692 291.7989 45.0251 0.486 1854 +1856 4 254.6544 291.2521 46.5273 0.3553 1855 +1857 4 254.3512 290.576 46.132 0.2844 1856 +1858 4 253.7392 289.9376 46.058 0.1661 1857 +1859 4 253.4326 289.2673 47.7621 0.275 1858 +1860 4 253.078 288.383 47.1654 0.2204 1859 +1861 4 252.7737 287.311 46.8532 0.2094 1860 +1862 4 252.3492 286.4862 47.0719 0.3208 1861 +1863 4 252.0426 285.7254 48.1449 0.2672 1862 +1864 4 251.9752 284.9143 49.3147 0.2288 1863 +1865 4 251.2064 284.2794 50.3468 0.2288 1864 +1866 4 250.5543 283.4043 50.0458 0.3202 1865 +1867 4 249.9937 282.997 48.16 0.2812 1866 +1868 4 249.4229 282.4628 49.287 0.3432 1867 +1869 4 249.1254 281.551 49.9022 0.2796 1868 +1870 4 248.5626 281.6242 48.8846 0.1683 1869 +1871 4 247.4918 281.4274 49.5628 0.2463 1870 +1872 4 246.7505 280.7982 49.3511 0.2515 1871 +1873 4 245.8525 280.3807 50.1315 0.281 1872 +1874 4 245.2816 279.8853 50.1273 0.2169 1873 +1875 4 244.5666 279.454 49.28 0.3051 1874 +1876 4 243.6812 278.7917 49.6138 0.2918 1875 +1877 4 243.0119 278.1613 50.6066 0.3382 1876 +1878 4 242.3106 277.4028 51.2865 0.2942 1877 +1879 4 241.9754 276.657 50.4053 0.2393 1878 +1880 4 241.4755 275.7978 50.8094 0.2587 1879 +1881 4 240.7239 275.1034 51.8507 0.178 1880 +1882 4 240.2343 274.2065 52.0618 0.2288 1881 +1883 4 239.7103 273.3828 52.1007 0.2203 1882 +1884 4 239.5833 272.4505 53.1185 0.3305 1883 +1885 4 238.9473 271.9196 52.7853 0.2432 1884 +1886 4 238.5938 271.1852 53.6505 0.1971 1885 +1887 4 237.8342 270.421 53.9773 0.1652 1886 +1888 4 237.0288 270.3855 54.3074 0.2339 1887 +1889 4 236.3229 269.7529 53.5836 0.2843 1888 +1890 4 235.672 269.0185 54.2688 0.2011 1889 +1891 4 235.0623 268.2451 53.7323 0.2161 1890 +1892 4 234.568 267.3791 53.5648 0.2474 1891 +1893 4 234.1368 266.6767 54.0492 0.211 1892 +1894 4 234.0464 266.4273 56.2489 0.229 1893 +1895 4 233.7192 265.3314 56.2489 0.2516 1894 +1896 4 232.8463 264.6518 56.0311 0.2193 1895 +1897 4 232.2423 264.0752 54.9332 0.217 1896 +1898 4 231.7206 263.2699 54.9489 0.2288 1897 +1899 4 231.0102 262.9015 55.5892 0.2543 1898 +1900 4 230.794 262.2483 55.9908 0.2587 1899 +1901 4 230.6007 261.3857 56.5454 0.3432 1900 +1902 4 230.0641 260.4819 55.5845 0.2424 1901 +1903 4 229.8296 259.6251 54.5714 0.4277 1902 +1904 4 229.5962 258.7854 54.6501 0.2934 1903 +1905 4 228.8092 258.3598 55.8144 0.3215 1904 +1906 4 228.5026 257.4057 56.4029 0.2382 1905 +1907 4 228.5884 256.5981 56.84 0.1876 1906 +1908 4 228.2589 255.5055 56.8851 0.1709 1907 +1909 4 227.5942 254.7322 57.3448 0.1963 1908 +1910 4 226.8598 254.1671 56.6222 0.2669 1909 +1911 4 226.1665 253.3377 56.9898 0.1986 1910 +1912 4 225.9045 252.379 57.12 0.1984 1911 +1913 4 225.3989 251.3608 57.3017 0.2666 1912 +1914 4 225.1449 250.7328 58.3472 0.2994 1913 +1915 4 224.6438 250.1505 59.6285 0.2255 1914 +1916 4 224.621 249.2078 59.0848 0.3197 1915 +1917 4 223.9094 248.5718 59.64 0.2767 1916 +1918 4 223.8808 247.9574 59.8441 0.3122 1917 +1919 4 223.0125 247.5994 59.3158 0.2549 1918 +1920 4 222.9656 246.6464 60.48 0.3051 1919 +1921 4 222.2849 246.1327 61.6753 0.1889 1920 +1922 4 221.4841 245.5882 61.88 0.1873 1921 +1923 4 221.0059 245.0402 60.1888 0.2364 1922 +1924 4 220.4705 244.8057 60.76 0.1271 1923 +1925 4 219.6491 244.1296 60.48 0.2288 1924 +1926 4 219.2224 243.7635 62.5162 0.2321 1925 +1927 4 218.6321 243.1309 63.4094 0.2669 1926 +1928 4 218.3736 242.3232 64.4028 0.3264 1927 +1929 4 217.5625 241.7592 64.3283 0.309 1928 +1930 4 216.8155 240.9344 64.12 0.3726 1929 +1931 4 216.0696 240.0947 64.3737 0.5053 1930 +1932 4 215.4655 239.3626 65.2288 0.5131 1931 +1933 4 215.0182 238.5366 66.0988 0.4272 1932 +1934 4 214.1248 237.9451 66.5414 0.3382 1933 +1935 4 213.3743 237.1054 66.6534 0.3432 1934 +1936 4 213.4693 236.236 66.9186 0.2671 1935 +1937 4 212.6204 235.6663 65.8 0.3358 1936 +1938 4 212.4374 234.7751 66.642 0.1922 1937 +1939 4 211.7498 234.3141 67.0466 0.3437 1938 +1940 4 211.2522 233.6048 67.7359 0.482 1939 +1941 4 210.5726 232.78 67.7757 0.3434 1940 +1942 4 209.9023 232.041 67.1216 0.3281 1941 +1943 4 209.4515 231.1921 66.9393 0.3765 1942 +1944 4 208.9951 230.3776 67.6855 0.353 1943 +1945 4 208.4814 229.69 68.88 0.4491 1944 +1946 4 207.9792 228.7222 68.698 0.2569 1945 +1947 4 207.5113 228.228 69.953 0.3875 1946 +1948 4 207.0137 227.4913 70.5186 0.2924 1947 +1949 4 206.3925 226.6939 70.74 0.4721 1948 +1950 4 205.5768 225.7146 68.9307 0.2469 1949 +1951 4 205.0071 225.0488 69.7799 0.3204 1950 +1952 4 204.355 224.2858 69.19 0.2288 1951 +1953 4 203.8883 223.7607 70.6776 0.3775 1952 +1954 4 203.6961 223.0514 70.6726 0.3268 1953 +1955 4 202.9788 222.6521 71.4692 0.472 1954 +1956 4 202.6138 221.6969 71.6864 0.3536 1955 +1957 4 201.9743 221.0116 72.142 0.3725 1956 +1958 4 201.36 220.1559 71.2379 0.2436 1957 +1959 4 201.1838 219.9088 72.266 0.1144 1958 +1960 4 200.812 219.171 73.67 0.1657 1959 +1961 4 200.2892 218.226 74.403 0.1977 1960 +1962 4 199.8465 217.2227 73.7769 0.2776 1961 +1963 4 199.5228 216.3704 72.6037 0.2765 1962 +1964 4 198.8009 215.5696 72.4802 0.394 1963 +1965 4 198.2403 214.6624 72.0619 0.2288 1964 +1966 4 198.0161 214.0893 74.1482 0.2818 1965 +1967 4 197.5082 213.3343 75.0126 0.4173 1966 +1968 4 196.9167 212.538 74.6911 0.2423 1967 +1969 4 196.5472 211.7372 75.3514 0.3432 1968 +1970 4 195.9706 211.0211 75.7884 0.4558 1969 +1971 4 195.6549 210.1459 75.7711 0.3156 1970 +1972 4 194.9628 209.7684 74.2092 0.4919 1971 +1973 4 194.5944 208.8052 75.0795 0.395 1972 +1974 4 194.2237 207.9346 75.8545 0.4263 1973 +1975 4 192.9001 206.7242 77.1 0.4235 1974 +1976 4 192.4151 206.031 77.5869 0.3298 1975 +1977 4 191.8946 205.3903 77.3111 0.2567 1976 +1978 4 191.5708 204.6536 77.28 0.2584 1977 +1979 4 190.8604 203.9763 77.9212 0.5339 1978 +1980 4 190.7689 203.4467 79.8238 0.3861 1979 +1981 4 190.2472 202.7317 80.0873 0.4454 1980 +1982 4 190.0276 201.7055 80.2984 0.3557 1981 +1983 4 189.2473 201.1518 80.0103 0.3756 1982 +1984 4 189.0071 200.3945 78.5932 0.3821 1983 +1985 4 188.7646 199.9083 80.1206 0.2245 1984 +1986 4 188.2612 199.2287 79.6816 0.3159 1985 +1987 4 187.3437 198.7471 79.002 0.4299 1986 +1988 4 186.6425 197.9566 79.469 0.3586 1987 +1989 4 185.9526 197.4601 80.3799 0.4454 1988 +1990 4 185.5728 197.1981 82.04 0.4195 1989 +1991 4 185.1312 196.1903 82.2343 0.3686 1990 +1992 4 184.3464 195.4181 82.7114 0.3313 1991 +1993 4 183.6074 194.623 83.4165 0.3811 1992 +1994 4 183.3294 193.5476 83.72 0.4602 1993 +1995 4 182.7197 192.732 83.5853 0.3615 1994 +1996 4 182.1214 191.8294 83.8956 0.353 1995 +1997 4 181.4773 191.1384 82.88 0.3813 1996 +1998 4 180.9716 190.2094 82.9116 0.3038 1997 +1999 4 180.315 189.4178 82.88 0.3704 1998 +2000 4 179.608 189.0014 83.54 0.2319 1999 +2001 4 179.3998 188.1811 84.5513 0.3051 2000 +2002 4 178.9124 187.33 84.6866 0.3691 2001 +2003 4 178.0201 186.758 84.9971 0.3392 2002 +2004 4 177.5728 185.8245 84.84 0.2953 2003 +2005 4 177.0923 184.8773 84.0062 0.4058 2004 +2006 4 176.6096 184.0833 84.0118 0.321 2005 +2007 4 175.8397 183.4953 84.5995 0.4174 2006 +2008 4 175.4873 182.9439 85.8561 0.3348 2007 +2009 4 174.9336 182.158 86.6746 0.2415 2008 +2010 4 174.6774 181.324 85.9636 0.4307 2009 +2011 4 174.3399 180.5953 87.08 0.3747 2010 +2012 4 173.6386 179.934 86.8935 0.4109 2011 +2013 4 173.3812 178.9754 86.8045 0.3424 2012 +2014 4 173.0769 178.3324 87.3152 0.2388 2013 +2015 4 172.8287 177.423 86.133 0.3051 2014 +2016 4 172.0576 176.6977 86.5354 0.4068 2015 +2017 4 171.6938 176.128 88.2837 0.3386 2016 +2018 4 171.1985 175.2608 89.0798 0.2352 2017 +2019 4 170.6619 174.6831 89.7005 0.3368 2018 +2020 4 170.1563 173.745 88.7788 0.2916 2019 +2021 4 169.5076 173.054 89.32 0.3295 2020 +2022 4 169.0729 172.3802 88.2059 0.3099 2021 +2023 4 168.3373 171.695 88.4366 0.1781 2022 +2024 4 167.5834 171.2614 89.6221 0.3887 2023 +2025 4 167.0675 170.5761 90.72 0.3487 2024 +2026 4 166.6922 169.5774 90.9504 0.2736 2025 +2027 4 166.0104 168.6805 90.8214 0.3615 2026 +2028 4 165.5334 167.8648 89.4449 0.4909 2027 +2029 4 164.776 167.2093 89.5843 0.4261 2028 +2030 4 164.2018 166.2575 89.6132 0.3665 2029 +2031 4 163.8826 165.6604 91.0482 0.337 2030 +2032 4 163.7007 164.641 90.5915 0.3375 2031 +2033 4 163.3964 163.616 91.0115 0.2341 2032 +2034 4 163.3312 162.7351 91.56 0.2229 2033 +2035 4 162.8347 161.9069 91.9881 0.3491 2034 +2036 4 162.0785 161.137 92.694 0.2981 2035 +2037 4 161.1759 160.5066 93.1837 0.2377 2036 +2038 4 160.7595 159.5045 93.2467 0.2034 2037 +2039 4 160.2561 158.5721 93.7045 0.2094 2038 +2040 4 159.7493 157.9235 93.0404 0.2898 2039 +2041 4 159.461 156.9625 93.5558 0.2677 2040 +2042 4 159.135 156.029 94.5098 0.2206 2041 +2043 4 158.6042 155.1664 95.1686 0.1738 2042 +2044 4 158.2884 154.0888 94.92 0.1192 2043 +2045 4 157.753 153.2171 95.2812 0.3227 2044 +2046 4 157.4236 152.1291 95.2 0.3284 2045 +2047 4 157.0575 151.0549 95.4198 0.2915 2046 +2048 4 156.6616 150.198 96.8103 0.2605 2047 +2049 4 156.2304 149.3995 97.7693 0.2601 2048 +2050 4 155.7579 148.5976 97.071 0.3911 2049 +2051 4 155.3323 147.7636 96.9422 0.3122 2050 +2052 4 154.8976 146.9548 98.4326 0.1401 2051 +2053 4 154.3245 146.0613 98.84 0.1144 2052 +2054 4 153.6049 145.3143 98.8308 0.1182 2053 +2055 4 152.8155 144.5398 98.4931 0.1898 2054 +2056 4 152.2458 143.5926 98.7792 0.1846 2055 +2057 4 151.5537 142.8616 99.12 0.3122 2056 +2058 4 150.7563 142.2644 98.7109 0.2288 2057 +2059 4 149.9933 141.8331 99.6128 0.2669 2058 +2060 4 149.7004 140.9419 100.6138 0.3392 2059 +2061 4 149.3698 139.9329 101.39 0.2084 2060 +2062 4 149.1593 138.8553 102.0124 0.124 2061 +2063 4 148.5187 137.9264 102.2 0.1144 2062 +2064 4 147.6549 137.3143 102.4666 0.1144 2063 +2065 4 146.9937 136.4243 102.48 0.1336 2064 +2066 4 146.4755 135.4599 102.7869 0.1725 2065 +2067 4 146.2146 134.4349 103.1414 0.1635 2066 +2068 4 145.7559 133.5448 103.9004 0.1144 2067 +2069 4 145.3418 132.5198 104.16 0.1144 2068 +2070 4 144.4769 131.8952 104.2272 0.1225 2069 +2071 4 143.7081 131.1173 104.4291 0.2116 2070 +2072 4 143.5617 130.5693 106.3866 0.167 2071 +2073 4 143.1201 129.7182 106.6985 0.1939 2072 +2074 4 142.7815 128.7927 106.2384 0.2288 2073 +2075 4 142.4257 127.8614 106.7878 0.1907 2074 +2076 4 142.0779 127.0458 107.8 0.1714 2075 +2077 4 141.7038 125.9922 107.7997 0.1907 2076 +2078 4 141.0678 125.109 107.8246 0.1325 2077 +2079 4 140.2292 124.5278 108.5468 0.1144 2078 +2080 4 139.4547 123.7064 108.64 0.1144 2079 +2081 4 138.8587 122.9651 109.482 0.1144 2080 +2082 4 138.5338 122.0202 109.9134 0.1312 2081 +2083 4 137.9515 121.089 110.2469 0.1191 2082 +2084 4 137.1919 120.3133 111.0547 0.1514 2083 +2085 4 136.7034 119.3112 111.517 0.2034 2084 +2086 4 136.16 118.3376 112.0 0.162 2085 +2087 4 136.033 117.3241 112.572 0.116 2086 +2088 4 136.2298 116.2876 113.3457 0.1341 2087 +2089 4 136.0857 115.1791 113.68 0.1487 2088 +2090 4 135.7676 114.0822 113.68 0.2288 2089 +2091 4 135.3981 113.5568 113.96 0.3382 2090 +2092 4 134.8776 112.7799 114.7471 0.3216 2091 +2093 4 134.8421 111.7206 115.0932 0.265 2092 +2094 4 135.4416 110.8978 115.4829 0.2255 2093 +2095 4 135.6933 110.0838 114.0 0.2614 2094 +2096 4 135.6784 109.1147 114.408 0.2728 2095 +2097 4 135.9644 108.3403 116.1345 0.2731 2096 +2098 4 135.7734 107.3832 116.247 0.2754 2097 +2099 4 135.8157 106.3778 116.713 0.1801 2098 +2100 4 135.7562 105.2764 117.2979 0.2305 2099 +2101 4 135.4313 104.1866 117.4362 0.2765 2100 +2102 4 135.1762 103.141 118.1471 0.2258 2101 +2103 4 134.6808 102.7232 116.5688 0.1973 2102 +2104 4 134.6305 101.8254 115.64 0.2256 2103 +2105 4 134.6488 100.7918 116.0944 0.1144 2104 +2106 4 134.6911 100.0884 117.6512 0.148 2105 +2107 4 134.7815 99.3526 118.72 0.3813 2106 +2108 4 135.2116 98.4157 118.2686 0.3241 2107 +2109 4 135.2437 97.7976 116.3408 0.2519 2108 +2110 4 135.54 96.8788 117.32 0.1947 2109 +2111 4 135.6384 96.0939 116.9518 0.2655 2110 +2112 4 135.5686 95.3231 116.0323 0.2111 2111 +2113 4 135.961 94.9299 114.4307 0.2341 2112 +2114 4 136.247 93.8935 113.96 0.2448 2113 +2115 4 136.3568 92.8218 114.1753 0.2625 2114 +2116 4 136.4529 91.8987 115.36 0.1849 2115 +2117 4 137.0512 91.0443 115.5636 0.2385 2116 +2118 4 136.9402 90.948 118.258 0.1332 2117 +2119 4 136.9368 90.9226 121.0527 0.1144 2118 +2120 4 137.0512 90.5832 123.489 0.1144 2119 +2121 4 137.0638 89.7208 125.1228 0.1144 2120 +2122 4 137.0215 89.0255 127.1393 0.1349 2121 +2123 4 137.0592 88.0054 127.9911 0.2262 2122 +2124 4 137.1805 86.96 128.7784 0.1232 2123 +2125 4 137.8566 86.1886 129.0635 0.1719 2124 +2126 4 138.0808 85.5975 129.8321 0.2235 2125 +2127 4 138.6082 84.7646 130.188 0.1144 2126 +2128 4 139.2008 83.8653 130.76 0.2266 2127 +2129 4 139.465 83.1791 129.3804 0.1773 2128 +2130 4 140.2121 82.5405 129.2609 0.1562 2129 +2131 4 140.8264 81.6683 129.08 0.1182 2130 +2132 4 141.6215 80.9534 129.0187 0.1144 2131 +2133 4 142.5664 80.3992 128.5889 0.1144 2132 +2134 4 143.1178 79.4848 128.3582 0.1144 2133 +2135 4 143.2288 78.4465 128.4875 0.1144 2134 +2136 4 143.0149 77.6787 129.3454 0.1675 2135 +2137 4 143.1522 77.233 131.0431 0.2472 2136 +2138 4 143.4702 76.4309 132.16 0.2478 2137 +2139 4 143.8706 75.5771 132.6601 0.2069 2138 +2140 4 143.7962 74.8564 134.1166 0.1254 2139 +2141 4 143.3032 74.0542 135.2425 0.1144 2140 +2142 4 143.0801 73.2361 136.2155 0.1468 2141 +2143 4 143.2174 72.1136 136.08 0.2382 2142 +2144 4 143.4576 71.3856 136.64 0.1398 2143 +2145 4 135.3993 113.6063 114.2098 0.3051 2090 +2146 4 134.6705 113.0582 113.1259 0.2772 2145 +2147 4 134.2839 112.9949 114.6107 0.3276 2146 +2148 4 133.7771 112.7324 116.48 0.2415 2147 +2149 4 133.0724 112.0937 117.7081 0.2775 2148 +2150 4 132.9751 111.1348 118.6234 0.2504 2149 +2151 4 132.3414 110.3951 118.454 0.1563 2150 +2152 4 131.56 110.0009 119.576 0.273 2151 +2153 4 131.2111 109.3296 121.2224 0.1453 2152 +2154 4 130.6368 108.7944 122.2931 0.2055 2153 +2155 4 129.9607 108.2954 121.2926 0.265 2154 +2156 4 129.5225 107.9852 122.703 0.1314 2155 +2157 4 129.725 107.3733 124.2497 0.1478 2156 +2158 4 129.2892 106.8544 123.7054 0.2288 2157 +2159 4 128.5948 106.3221 124.88 0.2314 2158 +2160 4 128.3431 105.2986 125.5346 0.1652 2159 +2161 4 127.4553 104.8079 126.28 0.1144 2160 +2162 4 126.8971 104.0031 127.073 0.2605 2161 +2163 4 125.9418 103.4019 127.12 0.2765 2162 +2164 4 125.1742 102.8517 127.9116 0.1907 2163 +2165 4 124.4523 102.6348 129.64 0.1271 2164 +2166 4 123.5692 101.9666 129.92 0.1333 2165 +2167 4 122.8233 101.3039 130.7348 0.1265 2166 +2168 4 121.8028 100.8097 130.76 0.131 2167 +2169 4 120.9437 100.4054 131.224 0.2008 2168 +2170 4 120.0754 99.7797 130.6836 0.1849 2169 +2171 4 119.4061 99.3095 129.1987 0.1302 2170 +2172 4 118.515 99.143 128.6659 0.1144 2171 +2173 4 117.6696 98.5148 128.24 0.1144 2172 +2174 4 116.5496 98.384 128.4363 0.1144 2173 +2175 4 115.409 98.384 128.52 0.1194 2174 +2176 4 114.5167 98.384 129.7778 0.1619 2175 +2177 4 113.8957 98.4984 132.0063 0.2288 2176 +2178 4 113.0108 98.9001 132.0852 0.2868 2177 +2179 4 112.0936 99.2735 131.3301 0.2418 2178 +2180 4 111.1802 99.3057 131.068 0.2135 2179 +2181 4 110.123 99.362 131.0397 0.2287 2180 +2182 4 109.268 99.4342 132.02 0.2431 2181 +2183 4 108.3749 99.0196 131.6311 0.3178 2182 +2184 4 107.9407 98.8416 132.7418 0.3199 2183 +2185 4 107.5813 98.384 132.7782 0.1477 2184 +2186 4 107.269 98.023 131.2394 0.2898 2185 +2187 4 106.4616 97.3663 131.6078 0.1263 2186 +2188 4 105.8261 97.391 133.2612 0.1144 2187 +2189 4 105.6227 96.6324 133.56 0.1271 2188 +2190 4 105.4783 95.6784 132.9051 0.1907 2189 +2191 4 104.7904 95.5017 131.4712 0.1504 2190 +2192 4 104.719 94.465 130.975 0.2004 2191 +2193 4 104.7546 93.4246 131.0512 0.1788 2192 +2194 4 104.9176 92.3282 131.0501 0.1566 2193 +2195 4 105.1714 91.5068 129.8564 0.1522 2194 +2196 4 104.9646 90.7354 129.5868 0.2288 2195 +2197 4 105.1305 90.376 131.8929 0.1715 2196 +2198 4 105.0192 89.9533 133.387 0.313 2197 +2199 4 104.676 89.1177 132.6399 0.1953 2198 +2200 4 104.4472 88.307 133.5298 0.2743 2199 +2201 4 104.3328 87.3038 132.4621 0.2692 2200 +2202 4 104.0619 86.414 130.9991 0.1921 2201 +2203 4 103.2351 85.8715 130.3224 0.2015 2202 +2204 4 103.0362 85.1773 128.8319 0.1907 2203 +2205 4 103.293 84.8392 127.1651 0.155 2204 +2206 4 103.6284 83.8574 126.84 0.1981 2205 +2207 4 102.9944 83.0582 127.132 0.1682 2206 +2208 4 102.325 82.3305 126.9036 0.3387 2207 +2209 4 102.1894 81.4099 126.5561 0.1382 2208 +2210 4 101.5258 80.9671 124.6666 0.2407 2209 +2211 4 101.2654 80.3004 123.9762 0.1228 2210 +2212 4 101.538 79.6142 124.1198 0.2542 2211 +2213 4 101.5872 78.8216 124.04 0.2542 2212 +2214 4 107.6236 98.956 132.314 0.249 2183 +2215 4 107.0173 99.6743 131.8738 0.2094 2214 +2216 4 106.4612 100.224 130.5581 0.1718 2215 +2217 4 105.7241 100.6961 131.5591 0.1652 2216 +2218 4 105.3258 101.2052 133.0 0.2547 2217 +2219 4 104.5105 101.914 132.7306 0.2672 2218 +2220 4 103.7482 102.3552 133.7563 0.1605 2219 +2221 4 103.0297 102.8874 134.3734 0.1922 2220 +2222 4 102.4516 103.6337 134.0268 0.2288 2221 +2223 4 101.816 103.9133 134.2132 0.2288 2222 +2224 4 101.6215 104.0517 134.9334 0.1416 2222 +2225 4 100.8323 104.4483 134.7819 0.2519 2224 +2226 4 100.2006 104.9818 135.4564 0.1777 2225 +2227 4 99.5304 105.5897 134.6374 0.1144 2226 +2228 4 99.1649 106.396 135.5973 0.142 2227 +2229 4 98.4944 106.9781 136.8906 0.1144 2228 +2230 4 97.8302 107.3853 138.1134 0.2188 2229 +2231 4 97.2462 107.7832 139.806 0.1657 2230 +2232 4 97.0504 108.4184 139.7536 0.2012 2231 +2233 4 96.6121 109.1376 140.4455 0.2718 2232 +2234 4 96.2954 109.7806 141.9558 0.2051 2233 +2235 4 95.3715 109.824 143.2668 0.2034 2234 +2236 4 94.4802 110.2816 143.5031 0.2134 2235 +2237 4 93.6863 110.4196 144.8868 0.1568 2236 +2238 4 93.1176 111.073 144.9384 0.1398 2237 +2239 4 92.3406 110.968 143.7419 0.2631 2238 +2240 4 91.5187 110.8489 145.4379 0.1954 2239 +2241 4 90.5336 110.5398 146.5738 0.1652 2240 +2242 4 89.9027 110.7393 148.6492 0.178 2241 +2243 4 88.9348 111.0824 147.9848 0.2539 2242 +2244 4 87.9145 110.9666 146.886 0.1586 2243 +2245 4 86.8543 111.2734 146.7357 0.1652 2244 +2246 4 85.9644 111.8899 147.499 0.1652 2245 +2247 4 85.1134 112.2264 148.4115 0.1905 2246 +2248 4 84.7254 112.2592 150.6086 0.1499 2247 +2249 4 83.9685 112.6759 151.5816 0.2261 2248 +2250 4 83.2832 112.4552 149.8 0.1525 2249 +2251 4 223.8064 247.0079 61.161 0.2799 1920 +2252 4 224.224 247.9048 59.92 0.2796 2251 +2253 4 248.3876 280.598 49.28 0.3178 1869 +2254 4 248.5306 279.4792 50.3552 0.2932 2253 +2255 4 249.3497 278.9861 51.3752 0.2613 2254 +2256 4 249.1907 278.0355 50.5926 0.1907 2255 +2257 4 249.5064 277.1512 51.711 0.242 2256 +2258 4 249.527 276.2806 52.5893 0.2756 2257 +2259 4 250.3232 275.6662 51.8263 0.138 2258 +2260 4 250.5463 274.6538 52.3508 0.2656 2259 +2261 4 250.9032 273.8999 52.4465 0.1332 2260 +2262 4 250.8792 272.812 52.92 0.1707 2261 +2263 4 251.227 271.8853 53.2734 0.2374 2262 +2264 4 251.6583 271.239 53.7068 0.2372 2263 +2265 4 251.7944 270.4576 54.2497 0.2288 2264 +2266 4 251.7944 269.5447 55.0642 0.2288 2265 +2267 4 251.68 268.8469 54.962 0.3373 2266 +2268 4 252.1902 268.165 55.5307 0.115 2267 +2269 4 252.7222 267.3551 54.7929 0.2075 2268 +2270 4 253.1478 266.5394 56.1554 0.2224 2269 +2271 4 253.7323 265.6059 56.203 0.2669 2270 +2272 4 253.396 264.725 56.5452 0.2542 2271 +2273 4 253.1066 263.7778 56.3046 0.2288 2272 +2274 4 253.6751 262.8489 56.3634 0.2504 2273 +2275 4 254.3112 262.2197 56.6989 0.3893 2274 +2276 4 254.6544 261.404 58.002 0.2911 2275 +2277 4 254.5812 260.3504 57.6265 0.1184 2276 +2278 4 253.9188 259.9088 56.7602 0.1326 2277 +2279 4 253.7312 259.3448 57.5523 0.3715 2278 +2280 4 253.865 258.5577 57.1096 0.178 2279 +2281 4 254.8043 258.417 57.148 0.323 2280 +2282 4 255.6325 258.0944 58.0398 0.2039 2281 +2283 4 256.3315 257.4389 58.3862 0.1652 2282 +2284 4 257.3451 257.154 58.7997 0.2627 2283 +2285 4 258.147 256.8612 60.1888 0.1785 2284 +2286 4 259.1412 256.4848 60.2 0.243 2285 +2287 4 259.8859 256.8989 61.3021 0.3069 2286 +2288 4 260.641 257.5144 62.6268 0.1907 2287 +2289 4 261.4395 258.1802 63.7185 0.2503 2288 +2290 4 261.9463 257.8656 65.1568 0.1144 2289 +2291 4 262.4691 258.3827 66.36 0.1144 2290 +2292 4 263.1543 258.7579 64.6996 0.1968 2291 +2293 4 264.0146 259.0165 63.9954 0.2161 2292 +2294 4 265.0065 259.4295 63.84 0.2722 2293 +2295 4 265.6746 259.5976 65.3114 0.269 2294 +2296 4 266.5074 259.4558 65.9789 0.2196 2295 +2297 4 267.0714 258.6778 66.6439 0.2637 2296 +2298 4 268.0232 258.6275 67.4579 0.1433 2297 +2299 4 268.6318 257.9663 67.7295 0.2168 2298 +2300 4 269.5687 257.376 68.2223 0.2438 2299 +2301 4 270.3261 256.8337 68.8433 0.1525 2300 +2302 4 270.1476 256.6084 66.3426 0.1834 2301 +2303 4 270.7871 256.5981 65.24 0.1525 2302 +2304 4 271.128 256.4699 66.9774 0.2399 2303 +2305 4 271.5822 256.3326 69.3199 0.3499 2304 +2306 4 272.4619 255.9757 69.1911 0.233 2305 +2307 4 272.9447 255.3934 70.7585 0.1144 2306 +2308 4 273.416 254.8409 70.0694 0.2082 2307 +2309 4 274.0704 254.2048 70.6558 0.2043 2308 +2310 4 275.0691 253.8067 70.5488 0.2692 2309 +2311 4 275.7189 252.951 70.8397 0.2028 2310 +2312 4 276.7176 252.7004 70.4701 0.1144 2311 +2313 4 277.2701 252.4808 72.3192 0.1144 2312 +2314 4 278.2803 252.1765 73.1259 0.1223 2313 +2315 4 279.311 251.7361 73.36 0.1673 2314 +2316 4 280.0798 250.9456 73.8038 0.1907 2315 +2317 4 280.5786 250.0315 74.1401 0.2049 2316 +2318 4 281.4801 249.6208 73.64 0.1314 2317 +2319 4 282.1104 248.9722 74.7093 0.2135 2318 +2320 4 282.1928 247.9677 74.8362 0.2397 2319 +2321 4 282.7385 247.255 75.9433 0.2288 2320 +2322 4 283.4706 246.8672 76.979 0.1185 2321 +2323 4 283.7028 245.7884 76.72 0.1144 2322 +2324 4 283.9294 244.6913 76.72 0.1144 2323 +2325 4 284.8308 244.0381 76.7138 0.1144 2324 +2326 4 285.3056 243.1229 76.44 0.1144 2325 +2327 4 285.4886 242.4159 77.8445 0.1507 2326 +2328 4 285.476 241.6082 78.1063 0.2577 2327 +2329 4 286.1659 240.7708 78.4 0.2346 2328 +2330 4 286.4793 239.8339 79.2714 0.1907 2329 +2331 4 287.4254 239.2973 79.3766 0.2703 2330 +2332 4 288.4093 238.9187 80.0892 0.1144 2331 +2333 4 288.6552 238.2758 82.0187 0.1994 2332 +2334 4 289.3336 238.1808 80.421 0.1743 2333 +2335 4 289.7626 237.6145 78.797 0.3321 2334 +2336 4 290.2408 237.7918 79.8389 0.1907 2335 +2337 4 290.8208 237.0265 81.3596 0.1907 2336 +2338 4 291.402 236.2612 82.88 0.1907 2337 +2339 4 291.768 235.8928 83.5344 0.1144 2338 +2340 4 292.2703 235.7292 85.9606 0.1144 2339 +2341 4 292.5723 234.9776 87.0643 0.1144 2340 +2342 4 292.6352 233.853 86.8 0.1144 2341 +2343 4 292.6352 232.709 86.8 0.1144 2342 +2344 4 292.6283 231.5868 87.0794 0.1405 2343 +2345 4 292.7496 230.7448 87.92 0.178 2344 +2346 4 291.1549 236.3698 82.885 0.1265 2338 +2347 4 290.7076 237.1066 84.3951 0.2151 2346 +2348 4 290.2328 237.7232 85.68 0.1525 2347 +2349 4 260.3744 257.5144 63.0 0.1652 2288 +2350 4 247.8796 280.4196 48.72 0.2415 2253 +2351 4 256.0112 296.6975 44.9501 0.3236 1849 +2352 4 254.9015 296.7364 45.6288 0.2929 2351 +2353 4 253.9497 296.8577 44.9366 0.3467 2352 +2354 4 253.0974 296.7479 45.8354 0.1963 2353 +2355 4 252.562 297.4995 45.8088 0.2974 2354 +2356 4 252.061 298.2625 46.1437 0.1754 2355 +2357 4 251.7178 298.9272 45.7472 0.281 2356 +2358 4 251.0188 299.7028 46.1378 0.2444 2357 +2359 4 250.8792 300.5288 45.9091 0.1265 2358 +2360 4 250.3484 300.9715 45.1931 0.1144 2359 +2361 4 249.4892 301.0676 46.3456 0.1811 2360 +2362 4 248.4951 300.9864 46.9482 0.1591 2361 +2363 4 247.5307 301.1443 46.9288 0.2089 2362 +2364 4 246.5 301.2198 46.762 0.178 2363 +2365 4 245.4887 301.6248 47.0109 0.1915 2364 +2366 4 244.6021 302.0492 46.2 0.1165 2365 +2367 4 244.3801 302.9049 46.5077 0.2556 2366 +2368 4 243.863 303.8075 47.2077 0.1602 2367 +2369 4 243.4729 304.5568 46.9384 0.1739 2368 +2370 4 243.4672 305.6127 46.7082 0.2855 2369 +2371 4 243.5542 306.4707 47.4116 0.3405 2370 +2372 4 243.577 307.3951 48.5206 0.3191 2371 +2373 4 243.9992 308.1318 48.7589 0.2129 2372 +2374 4 243.5576 308.8972 48.5593 0.1179 2373 +2375 4 243.5576 309.6362 46.7286 0.2052 2374 +2376 4 243.2144 310.4267 45.4168 0.1329 2375 +2377 4 243.434 311.3648 45.8693 0.2063 2376 +2378 4 243.4066 312.0512 47.6 0.2262 2377 +2379 4 243.0108 313.0728 47.1414 0.1305 2378 +2380 4 242.5349 313.9571 46.5548 0.2086 2379 +2381 4 242.1676 315.0187 46.4145 0.2542 2380 +2382 4 241.8256 315.99 45.876 0.1723 2381 +2383 4 241.1884 316.8388 45.64 0.2481 2382 +2384 4 240.1233 317.1145 45.6218 0.1734 2383 +2385 4 239.3202 317.3811 45.1825 0.1563 2384 +2386 4 238.349 317.6934 45.1732 0.1953 2385 +2387 4 237.7953 318.612 45.5972 0.1612 2386 +2388 4 237.2816 319.5112 44.8636 0.1991 2387 +2389 4 236.4648 320.185 44.8146 0.1443 2388 +2390 4 235.7201 320.6666 44.24 0.1144 2389 +2391 4 235.1606 321.6642 44.24 0.1144 2390 +2392 4 234.544 322.5565 43.701 0.1144 2391 +2393 4 234.099 323.5735 43.96 0.1144 2392 +2394 4 233.9354 324.6786 43.7427 0.1144 2393 +2395 4 233.1426 325.2792 43.68 0.1144 2394 +2396 4 232.7811 326.3386 43.615 0.1144 2395 +2397 4 232.0032 326.9792 43.4 0.1144 2396 +2398 4 231.6714 328.002 43.4 0.1144 2397 +2399 4 231.3637 329.0762 43.4 0.1144 2398 +2400 4 231.1429 330.1264 43.209 0.1144 2399 +2401 4 230.7723 330.7579 42.6717 0.2008 2400 +2402 4 230.6304 331.7543 43.4437 0.1678 2401 +2403 4 230.5961 332.8846 43.12 0.1398 2402 +2404 4 230.341 333.9828 43.4 0.1489 2403 +2405 4 229.9543 335.0318 43.12 0.1244 2404 +2406 4 229.7484 336.1381 43.12 0.1144 2405 +2407 4 229.5711 337.2649 43.12 0.1144 2406 +2408 4 229.4864 338.346 42.5877 0.1144 2407 +2409 4 229.0723 339.3539 43.12 0.1144 2408 +2410 4 229.0288 340.4544 43.12 0.1144 2409 +2411 4 290.1184 350.8454 35.0 0.3686 1682 +2412 4 290.4113 349.9439 34.1653 0.2466 2411 +2413 4 289.8027 349.4085 33.7159 0.1479 2412 +2414 4 290.2328 348.7106 35.1868 0.3321 2413 +2415 4 290.4948 347.7119 35.56 0.2963 2414 +2416 4 290.0166 346.8482 34.7208 0.3306 2415 +2417 4 289.8267 345.9353 35.4668 0.2613 2416 +2418 4 289.7752 345.0373 33.8705 0.1435 2417 +2419 4 289.6608 343.9882 33.1139 0.2542 2418 +2420 4 289.7798 343.0753 33.6806 0.208 2419 +2421 4 289.7752 342.175 34.5044 0.2156 2420 +2422 4 290.1378 341.1282 34.44 0.1179 2421 +2423 4 290.1184 340.6603 32.5077 0.3472 2422 +2424 4 290.1184 339.768 33.32 0.2415 2423 +2425 4 290.29 339.4065 32.8076 0.2082 2424 +2426 4 290.6492 339.196 30.3363 0.2288 2425 +2427 4 291.299 339.5323 31.306 0.2288 2426 +2428 4 291.8321 339.871 30.9137 0.3713 2427 +2429 4 292.7748 339.9911 31.1419 0.2288 2428 +2430 4 293.3308 340.0151 32.2 0.2321 2429 +2431 4 293.7872 340.7679 30.8314 0.2122 2430 +2432 4 294.5869 340.8296 30.081 0.3149 2431 +2433 4 295.3888 341.2632 30.7476 0.2161 2432 +2434 4 296.3132 341.1397 29.7128 0.2791 2433 +2435 4 297.4194 340.9131 29.96 0.1788 2434 +2436 4 298.1447 340.9989 28.5631 0.2661 2435 +2437 4 298.9192 341.6213 29.1942 0.2849 2436 +2438 4 299.9053 342.0423 29.986 0.2629 2437 +2439 4 300.6821 341.3993 29.7195 0.3051 2438 +2440 4 301.6614 341.2186 29.3048 0.1683 2439 +2441 4 302.6864 340.912 29.68 0.3548 2440 +2442 4 303.303 340.2199 28.4917 0.341 2441 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4 331.2807 312.5614 23.4982 0.2221 2486 +2488 4 332.2885 312.185 22.6657 0.2995 2487 +2489 4 333.2312 311.6062 22.5624 0.2161 2488 +2490 4 334.1624 311.2355 22.414 0.2334 2489 +2491 4 335.152 311.3224 21.9624 0.3368 2490 +2492 4 336.1552 311.7743 21.3206 0.2343 2491 +2493 4 336.8519 311.6348 22.0352 0.2195 2492 +2494 4 337.5967 310.9827 21.1904 0.2548 2493 +2495 4 338.4181 310.7218 21.2789 0.2592 2494 +2496 4 339.4648 310.596 21.4693 0.3053 2495 +2497 4 340.2256 310.1407 21.5516 0.3298 2496 +2498 4 340.9555 309.5687 22.526 0.2209 2497 +2499 4 341.6053 309.3342 21.0 0.1652 2498 +2500 4 342.628 308.9178 20.6268 0.2288 2499 +2501 4 343.4196 309.7483 21.2587 0.1592 2500 +2502 4 344.0706 310.6463 21.7641 0.1398 2501 +2503 4 344.7581 311.4712 22.0494 0.1639 2502 +2504 4 345.8461 311.1543 22.1822 0.1652 2503 +2505 4 346.9741 311.1394 21.947 0.152 2504 +2506 4 347.9247 310.8591 21.7479 0.1373 2505 +2507 4 348.5448 309.8982 21.7862 0.1301 2506 +2508 4 348.7884 308.832 21.6224 0.1271 2507 +2509 4 348.8273 307.7017 21.5869 0.1334 2508 +2510 4 348.7621 306.5794 21.5572 0.1464 2509 +2511 4 348.2588 305.6951 21.3786 0.1525 2510 +2512 4 348.0597 303.6634 19.4102 0.3116 2511 +2513 4 348.8971 302.9335 19.32 0.3178 2512 +2514 4 349.7963 302.27 19.465 0.287 2513 +2515 4 350.6921 301.7231 19.7728 0.2712 2514 +2516 4 351.3052 301.6842 19.8923 0.2148 2515 +2517 4 352.0157 300.9338 19.5054 0.2796 2516 +2518 4 352.4344 300.0163 20.16 0.1814 2517 +2519 4 352.6792 299.1194 19.88 0.1584 2518 +2520 4 353.2786 298.7041 19.0756 0.1398 2519 +2521 4 353.6035 297.6985 19.3934 0.2977 2520 +2522 4 353.5692 296.6289 18.7415 0.3153 2521 +2523 4 353.4285 295.7526 18.6782 0.1255 2522 +2524 4 353.48 294.8912 19.4062 0.3417 2523 +2525 4 353.1528 293.8947 19.206 0.2313 2524 +2526 4 353.2306 292.9178 18.76 0.3296 2525 +2527 4 353.178 291.8561 19.32 0.1861 2526 +2528 4 353.7442 290.8929 19.6078 0.1939 2527 +2529 4 353.9399 289.8061 20.0869 0.2208 2528 +2530 4 354.0783 288.7307 20.72 0.2025 2529 +2531 4 354.64 288.288 19.32 0.1525 2530 +2532 4 343.1371 308.4258 20.5128 0.2366 2500 +2533 4 290.0921 338.4604 34.3658 0.1388 2424 +2534 4 290.0681 337.6962 32.3868 0.2924 2533 +2535 4 290.1047 337.4777 31.3883 0.1519 2534 +2536 4 289.4103 336.7959 30.8266 0.2706 2535 +2537 4 289.3588 335.8052 31.08 0.2542 2536 +2538 4 289.3176 334.8888 29.9827 0.2542 2537 +2539 4 289.5372 334.2219 28.9201 0.3969 2538 +2540 4 289.3142 333.4131 29.8189 0.2867 2539 +2541 4 289.0122 332.5722 30.6541 0.2061 2540 +2542 4 288.2491 331.9064 30.4088 0.131 2541 +2543 4 288.0958 331.1594 29.0746 0.1525 2542 +2544 4 287.9448 330.5405 29.972 0.2796 2543 +2545 4 287.819 329.7534 28.651 0.2039 2544 +2546 4 287.6222 328.9275 28.2212 0.2415 2545 +2547 4 287.2344 327.9974 28.0 0.2619 2546 +2548 4 286.4061 327.43 28.5463 0.1818 2547 +2549 4 285.3788 327.1485 27.9919 0.2734 2548 +2550 4 284.8766 326.3946 26.9802 0.3022 2549 +2551 4 284.0049 325.8123 26.8688 0.2417 2550 +2552 4 283.4809 325.0962 26.9111 0.2415 2551 +2553 4 283.1148 324.2988 27.1603 0.1528 2552 +2554 4 282.2671 324.0929 26.476 0.117 2553 +2555 4 281.2982 323.752 26.6874 0.1669 2554 +2556 4 280.4196 323.8161 26.63 0.1611 2555 +2557 4 279.5787 323.3745 26.857 0.2408 2556 +2558 4 278.4976 323.0702 26.6916 0.2163 2557 +2559 4 277.6259 322.9489 25.76 0.2853 2558 +2560 4 276.9498 322.2396 26.3388 0.2186 2559 +2561 4 275.9774 322.0703 25.7673 0.2288 2560 +2562 4 274.981 321.9559 24.6616 0.3012 2561 +2563 4 274.2431 321.8312 25.9028 0.2474 2562 +2564 4 273.1449 321.9216 25.716 0.3025 2563 +2565 4 272.4242 322.5028 24.638 0.2892 2564 +2566 4 271.748 322.5508 22.7035 0.2638 2565 +2567 4 270.8477 322.6892 23.0023 0.2004 2566 +2568 4 269.857 322.862 22.4932 0.1907 2567 +2569 4 269.412 322.8162 22.7172 0.2383 2568 +2570 4 268.9544 322.4936 23.8 0.1398 2569 +2571 4 269.2713 322.9512 22.4319 0.24 2568 +2572 4 268.284 323.2006 22.0886 0.2415 2571 +2573 4 267.3356 323.1892 21.7022 0.2669 2572 +2574 4 266.3507 322.989 21.1994 0.2325 2573 +2575 4 265.8301 323.4969 22.8928 0.1907 2574 +2576 4 265.2982 323.9888 21.6639 0.2246 2575 +2577 4 265.1872 324.7564 21.0683 0.1221 2576 +2578 4 264.6083 325.3536 21.2783 0.2289 2577 +2579 4 263.8007 325.6396 21.0157 0.1779 2578 +2580 4 262.6956 325.5515 21.0356 0.3021 2579 +2581 4 261.7072 325.5927 21.4749 0.1282 2580 +2582 4 260.9281 325.7517 21.7305 0.2034 2581 +2583 4 260.0575 325.7746 21.0339 0.1907 2582 +2584 4 259.3448 325.905 21.3052 0.1456 2583 +2585 4 258.7797 326.2139 19.3486 0.1671 2584 +2586 4 257.7661 326.1498 19.1464 0.2288 2585 +2587 4 257.4 326.3821 20.4674 0.1144 2586 +2588 4 256.8108 325.7929 20.6774 0.2033 2587 +2589 4 256.1473 325.2403 19.0994 0.2658 2588 +2590 4 255.0914 325.1648 19.0725 0.2533 2589 +2591 4 254.7345 324.6089 18.1877 0.2615 2590 +2592 4 253.7872 324.1959 17.8245 0.2412 2591 +2593 4 252.9567 323.5198 17.92 0.1234 2592 +2594 4 252.0461 323.0873 17.5305 0.1429 2593 +2595 4 251.1046 323.6079 17.3564 0.2542 2594 +2596 4 250.3266 323.172 16.3918 0.2288 2595 +2597 4 249.3028 322.8757 17.2875 0.1718 2596 +2598 4 248.3166 322.9523 16.9501 0.2277 2597 +2599 4 247.7904 322.9512 15.68 0.2669 2598 +2600 4 289.4446 338.2614 32.2 0.2674 2534 +2601 4 289.7752 339.3104 31.92 0.2669 2600 +2602 4 293.2598 361.4102 35.4603 0.3043 1673 +2603 4 293.5035 361.5601 38.1721 0.1891 2602 +2604 4 294.1533 361.7282 39.6091 0.1271 2603 +2605 4 294.9987 362.4787 39.6463 0.138 2604 +2606 4 295.1612 363.4911 39.6082 0.1811 2605 +2607 4 294.4782 364.3514 39.48 0.2161 2606 +2608 4 293.9394 364.8811 39.8709 0.1565 2607 +2609 4 293.4234 364.4143 42.0932 0.2669 2608 +2610 4 293.436 364.7861 41.6783 0.1652 2609 +2611 4 293.7609 365.6224 41.16 0.1485 2610 +2612 4 294.572 366.2596 41.461 0.1923 2611 +2613 4 295.1589 367.2274 41.7816 0.3514 2612 +2614 4 295.4952 368.2708 42.1366 0.3822 2613 +2615 4 295.6096 369.385 42.5919 0.2795 2614 +2616 4 296.2331 370.0588 43.7928 0.185 2615 +2617 4 296.5317 371.0942 43.7464 0.1497 2616 +2618 4 297.3256 371.8 43.4 0.2288 2617 +2619 4 292.4053 364.936 41.7189 0.3181 2609 +2620 4 291.8802 365.0504 43.143 0.3432 2619 +2621 4 290.7728 365.0321 43.5554 0.286 2620 +2622 4 289.8976 364.7072 42.7706 0.2392 2621 +2623 4 289.1368 364.7358 42.8515 0.2048 2622 +2624 4 288.24 364.1386 43.5266 0.3156 2623 +2625 4 287.3602 363.4614 43.68 0.4449 2624 +2626 4 286.3112 363.5632 44.5917 0.3148 2625 +2627 4 285.3834 363.832 45.808 0.1718 2626 +2628 4 284.3172 363.6776 45.6646 0.2382 2627 +2629 4 283.4443 363.0793 45.7114 0.272 2628 +2630 4 282.5383 362.9866 46.6374 0.194 2629 +2631 4 281.6173 362.6926 46.7496 0.1398 2630 +2632 4 280.8188 363.403 46.6312 0.3362 2631 +2633 4 279.7824 363.5174 47.031 0.3419 2632 +2634 4 279.1463 362.9912 48.0472 0.3432 2633 +2635 4 278.3947 362.4501 48.5447 0.2644 2634 +2636 4 277.5367 362.4192 50.2354 0.1211 2635 +2637 4 276.411 362.4718 50.4969 0.1662 2636 +2638 4 275.3379 362.6789 49.9425 0.2415 2637 +2639 4 274.4902 363.1399 49.84 0.2924 2638 +2640 4 273.877 363.1319 51.6174 0.3344 2639 +2641 4 273.0522 362.5588 51.9669 0.2843 2640 +2642 4 272.2491 362.5588 50.4311 0.2571 2641 +2643 4 271.3774 362.6308 51.52 0.1708 2642 +2644 4 270.2677 362.4215 51.52 0.1165 2643 +2645 4 269.2999 362.1618 50.7111 0.2004 2644 +2646 4 268.7084 362.1801 52.0719 0.2321 2645 +2647 4 267.7589 361.7454 51.333 0.2797 2646 +2648 4 266.7808 361.5418 51.7672 0.2789 2647 +2649 4 266.3209 361.6882 53.6942 0.3985 2648 +2650 4 265.4057 362.0474 53.4526 0.2439 2649 +2651 4 264.574 362.489 53.4411 0.2783 2650 +2652 4 263.954 362.7624 55.3714 0.1711 2651 +2653 4 263.1749 362.3746 55.6721 0.2334 2652 +2654 4 262.5286 362.8642 54.3262 0.2913 2653 +2655 4 261.6545 362.9866 55.9026 0.1447 2654 +2656 4 260.84 363.6559 56.5356 0.1864 2655 +2657 4 260.0243 364.1466 55.44 0.2384 2656 +2658 4 259.3494 364.7072 56.467 0.1685 2657 +2659 4 258.9787 365.532 57.4 0.1652 2658 +2660 4 258.989 366.4575 57.4445 0.323 2659 +2661 4 258.6332 367.1954 58.905 0.2582 2660 +2662 4 258.7213 367.6599 60.2 0.2539 2661 +2663 4 258.2591 368.4241 60.944 0.3108 2662 +2664 4 257.567 367.9161 59.5952 0.1271 2663 +2665 4 257.019 368.1117 58.0854 0.1233 2664 +2666 4 256.3876 368.9011 58.2106 0.1144 2665 +2667 4 256.256 370.0314 58.24 0.1144 2666 +2668 4 255.6863 370.4364 59.7954 0.1144 2667 +2669 4 254.9312 370.5988 61.8593 0.1144 2668 +2670 4 254.1751 370.7624 63.9234 0.1144 2669 +2671 4 253.976 371.7714 64.1962 0.1144 2670 +2672 4 253.7495 372.8788 64.1962 0.1144 2671 +2673 4 253.2553 373.897 64.1962 0.1144 2672 +2674 4 252.1387 373.6487 64.1962 0.1144 2673 +2675 4 251.203 373.3684 65.2669 0.1144 2674 +2676 4 250.401 373.063 67.118 0.1144 2675 +2677 4 249.5041 373.055 68.2506 0.1144 2676 +2678 4 248.4562 373.516 68.2506 0.1144 2677 +2679 4 247.4701 373.6533 68.2506 0.1144 2678 +2680 4 246.6922 372.9715 68.9321 0.1144 2679 +2681 4 245.9669 372.5036 70.7717 0.1144 2680 +2682 4 245.1981 372.0196 72.305 0.1144 2681 +2683 4 244.2074 371.4476 72.305 0.1144 2682 +2684 4 243.2167 370.8745 72.305 0.1144 2683 +2685 4 242.226 370.3025 72.305 0.1144 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b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/AMPA_ExcToExc.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 1.0, + "tau2": 3.0, + "erev": 0.0 +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/AMPA_ExcToInh.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/AMPA_ExcToInh.json new file mode 100644 index 00000000..43887996 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/AMPA_ExcToInh.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 0.1, + "tau2": 0.5, + "erev": 0.0 +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/GABA_InhToExc.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/GABA_InhToExc.json new file mode 100644 index 00000000..702ce9b4 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/GABA_InhToExc.json @@ -0,0 +1,7 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 2.7, + "tau2": 15.0, + "erev": -70.0 +} + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/GABA_InhToInh.json b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/GABA_InhToInh.json new file mode 100644 index 00000000..ed4130ab --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/components/synaptic_models/GABA_InhToInh.json @@ -0,0 +1,7 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 0.2, + "tau2": 8.0, + "erev": -70.0 +} + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/config.csv_spikes.json b/docs/tutorial/03_opt_advanced_spiking_inputs/config.csv_spikes.json new file mode 100644 index 00000000..3ba06e88 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/config.csv_spikes.json @@ -0,0 +1,69 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_csv_spikes", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": 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+ "nodes": [ + { + "nodes_file": "$NETWORK_DIR/cortex_nodes.h5", + "node_types_file": "$NETWORK_DIR/cortex_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/inputs_nodes.h5", + "node_types_file": "$NETWORK_DIR/inputs_node_types.csv" + } + ], + + "edges": [ + { + "edges_file": "$NETWORK_DIR/inputs_cortex_edges.h5", + "edge_types_file": "$NETWORK_DIR/inputs_cortex_edge_types.csv" + } + ] + } +} diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/config.nwb_inputs.json b/docs/tutorial/03_opt_advanced_spiking_inputs/config.nwb_inputs.json new file mode 100644 index 00000000..5754d536 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/config.nwb_inputs.json @@ -0,0 +1,122 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_nwb_inputs", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_nwb_inputs", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "LGN_spikes_sonata": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$INPUT_DIR/external_spikes.h5", + "node_set": "LGN" + }, + + "VISl_spikes_nwb": { + "input_type": "spikes", + "module": "ecephys_probe", + "input_file": "./ecephys_cache_dir/session_715093703/session_715093703.nwb", + "node_set": "VISl", + "interval": { + "interval_name": "drifting_gratings", + "interval_index": 0, + "temporal_frequency": 4.0, + "orientation": 90 + }, + "mapping": "sample", + "units": { + "location": "VISl" + } + }, + "Hipp_spikes_nwb": { + "input_type": "spikes", + "module": "ecephys_probe", + "input_file": "./ecephys_cache_dir/session_715093703/session_715093703.nwb", + "node_set": "Hippocampus", + "interval": { + "interval_name": "drifting_gratings", + "interval_index": 0, + "temporal_frequency": 4.0, + "orientation": 90 + }, + "mapping": "sample", + "units": { + "location": ["CA1", "CA2", "CA3", "Po"] + } + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv" + }, + + "components": { + "morphologies_dir": "$COMPONENT_DIR/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "$COMPONENT_DIR/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/VISp_nodes.h5", + "node_types_file": "$NETWORK_DIR/VISp_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/LGN_nodes.h5", + "node_types_file": "$NETWORK_DIR/LGN_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/VISl_nodes.h5", + "node_types_file": "$NETWORK_DIR/VISl_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/Hippocampus_nodes.h5", + 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a/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.nwb_inputs.py b/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.nwb_inputs.py new file mode 100644 index 00000000..e8b3f99e --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.nwb_inputs.py @@ -0,0 +1,24 @@ +import sys +from bmtk.simulator import bionet + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + net = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=net) + sim.run() + + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + # Find the appropriate config.json file + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + run('config.nwb_inputs.json') + # run('config.simulation.units_map.json') + # run('config.simulation.multi_session.json') diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.spikes_generator.py b/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.spikes_generator.py new file mode 100644 index 00000000..a19d9ffb --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.spikes_generator.py @@ -0,0 +1,36 @@ +import sys +from bmtk.simulator import bionet +from bmtk.simulator.bionet.io_tools import io +from bmtk.simulator.bionet import spikes_generator +import numpy as np + + +@spikes_generator +def my_spikes_generator(node, sim): + io.log_info(f'Generating custom spike trains for {node.node_id} from {node.population_name}') + if node['pop_name'] == 'tON': + return np.arange(100.0, sim.tstop, step=sim.dt*10) + elif node['pop_name'] == 'tOFF': + return np.arange(100.0, sim.tstop, step=sim.dt*20) + else: + return [] + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + net = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=net) + sim.run() + + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + # Find the appropriate config.json file + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + run('config.spikes_generator.json') + diff --git a/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.spont_syns.py b/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.spont_syns.py new file mode 100644 index 00000000..1c9ba5d6 --- /dev/null +++ b/docs/tutorial/03_opt_advanced_spiking_inputs/run_bmtk.spont_syns.py @@ -0,0 +1,23 @@ +import sys +from bmtk.simulator import bionet + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + net = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=net) + sim.run() + + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + # Find the appropriate config.json file + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + run('config.spont_syns.json') + diff --git a/docs/tutorial/04_opt_advanced_inputs/Ch_advanced_stim.ipynb b/docs/tutorial/04_opt_advanced_inputs/Ch_advanced_stim.ipynb new file mode 100644 index 00000000..e5672d87 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/Ch_advanced_stim.ipynb @@ -0,0 +1,1421 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "ea250b77-a92e-4946-9b2b-fefcae6f16d2", + "metadata": {}, + "source": [ + "# Advanced Stimulus Options\n", + "\n", + "In this tutorial, we will explain additional options for loading and generating advanced current-clamp wave forms, applying voltage clamps to cells during simulation, imporyting experimental sweeps from the Allen Cell-Types Database and adding extracellular stimulation to your simulated network.\n", + "\n", + "**Note** - scripts and files for running this tutorial can be found in the directory [04_opt_advanced_inputs/](https://github.com/AllenInstitute/bmtk/tree/develop/docs/tutorial/04_opt_advanced_inputs)" + ] + }, + { + "cell_type": "markdown", + "id": "7f4aa205-0e7a-4370-8db9-997a12bafe9b", + "metadata": {}, + "source": [ + "---\n", + "\n", + "## Contents\n", + "1. [Example: Advanced options when using current-clamp stimulation](#example-advance-icalmp)\n", + "2. [Example: Using current-clamp with complex and customized waveforms](#example-iclamp-waveforms)\n", + "3. [Example: Applying voltage-clamps to cells during simulation](#example-voltage-clamps)\n", + "4. [Example: Using sweep data from Allen Cell-Types In-vivo experiments](#example-cell-types)\n", + "5. [Example: Stimulating a network with extracellular field potentials](#example-xstim)\n", + "\n", + "---" + ] + }, + { + "cell_type": "markdown", + "id": "71f5e6f7-a04e-4c31-98a2-026cdfb6655a", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 1. Example: Advanced IClamp Options " + ] + }, + { + "cell_type": "markdown", + "id": "eb8682bf-5e2a-44ee-8d8d-9d73edd62f9b", + "metadata": {}, + "source": [ + "By default you can insert a simple step current into your simulation by adding to the \"inputs\" section of your SONATA configuration file. For example, say we want to insert a .100 nA injection at for 1000 ms into the soma of our first cell:\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp\": {\n", + " \"input_type\": \"clamp\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\"\n", + " \"node_id\": 0\n", + " },\n", + " \"amp\": 0.1000,\n", + " \"delay\": 500.0,\n", + " \"duration\": 1000.0,\n", + " \"section_name\": \"soma\",\n", + " \"section_index\": 0,\n", + " \"section_dist\": 0.5\n", + " }\n", + "}\n", + "```\n", + "* **input_typ** and **module** should always be set to values `clamp` and `IClamp`, respecitvely.\n", + "* The **node_set** specifies what cell(s) we want to apply clamp to. In this case only cell with node-id #0 in the \"net\" population of cells.\n", + "* **amp** is the amplitude of the clamp to apply, in nA.\n", + "* **delay** and **duration** tells us when to start the clamp and how long to apply it for, both in ms. In this case it will be a current from 500 - 1500 ms after start of simulation.\n", + "* **section_name**, **section_index**, and **section_dist** tells us exactly where on the cell to place the clamp. In this case at the center of the soma. (*If you leave section_index and section_dist blank BMTK will attempt to find a random avaialble place where to insert the clamp*)\n", + "
\n", + " NOTE: For PoinNet users section_name, section_index and section_dist is not applicable and can be removed.\n", + "
\n" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "192123e0-d03d-4388-a6dd-afc5e47163d3", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-05-05 10:30:21,328 [INFO] Created log file\n", + "2024-05-05 10:30:21,425 [INFO] Building cells.\n", + "2024-05-05 10:30:22,432 [INFO] Building recurrent connections\n", + "2024-05-05 10:30:22,441 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n", + "2024-05-05 10:30:22,442 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-05-05 10:30:22,443 [INFO] Block save every 5000 steps\n", + "2024-05-05 10:30:23,460 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-05-05 10:30:24,561 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-05-05 10:30:25,690 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-05-05 10:30:26,812 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-05-05 10:30:26,836 [INFO] Simulation completed in 4.394 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.current_clamp.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "34290b9c-9e00-4773-9714-ecf597d0390e", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "_ = plot_traces(config_file='config.current_clamp.json', node_ids=range(5), report_name='membrane_potential')" + ] + }, + { + "cell_type": "markdown", + "id": "1d3d422c-8ec2-43cb-a4b7-0201d062624f", + "metadata": {}, + "source": [ + "#### Selecting multiple cells\n", + "\n", + "In the above cells #1-4 have a flat trace since we only applied the current to cell #0. If you want to apply the same current to a specific population of cells you can use the **node_set** option to filter according to any available attributes. For example, say we want to apply the current cells in our network that have **model_type=biophysical**:\n", + "\n", + "```json\n", + "\"current_clamp\": {\n", + " \"input_type\": \"current_clamp\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"amp\": 0.1000,\n", + " \"delay\": 500.0,\n", + " \"duration\": 1000.0,\n", + " \"section_name\": \"soma\"\n", + "}\n", + "```" + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "id": "41309ea0-4432-4c78-88bc-872b26ebde4e", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-05-05 10:38:22,112 [INFO] Created log file\n", + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-05-05 10:38:22,130 [INFO] Building cells.\n", + "2024-05-05 10:38:23,128 [INFO] Building recurrent connections\n", + "2024-05-05 10:38:23,141 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n", + "2024-05-05 10:38:23,142 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-05-05 10:38:23,143 [INFO] Block save every 5000 steps\n", + "2024-05-05 10:38:24,566 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-05-05 10:38:25,905 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-05-05 10:38:27,381 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-05-05 10:38:28,682 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-05-05 10:38:28,700 [INFO] Simulation completed in 5.559 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.current_clamp_allcells.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "7472d4d5-754e-4179-b6ce-20816ba48272", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "_ = plot_traces(config_file='config.current_clamp.json', node_ids=range(5), report_name='membrane_potential')" + ] + }, + { + "cell_type": "markdown", + "id": "40176a68-188b-4f2f-9a99-2fa8f9cf4112", + "metadata": {}, + "source": [ + "#### Selecting different placement\n", + "\n", + "Besides the `soma`, most morphologically detailed will also give you options to place a clamp at the `axon`, `dend` (aka `basal`, the basal dendrites), or the `apic` (the apical dendrites). However unlike the `soma`, these other sections may be split into hundreds of different **section_index** (aka branches and compartments). If you do not know the specific section index for where you want to place the clamp you can use specify a range (in microns from the soma) of where to find select the current.\n", + "\n", + "For example if we want to place the current at a random location on the apical or basal dendrites that is atleast 500 um away from the soma\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp_apic\": {\n", + " \"input_type\": \"current_clamp\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"node_id\": [0]\n", + " },\n", + " \"amp\": 0.1000,\n", + " \"delay\": 500.0,\n", + " \"duration\": 1000.0,\n", + " \"section_name\": [\"dend\", \"apic\"],\n", + " \"section_index\": [500.0, 1.0e20]\n", + " }\n", + "}\n", + "```\n", + "* We are trying to find any section that is (500, $\\infty$) away from the soma. However python doesn't handle infinity values very well so we just use a distance, 1e20, that we know will cover all feasible segments\n", + "\n", + "
\n", + " WARNING: As metioned before this won't work on point-neurons or neurons with only a soma. Some models may not have an \"apic\" section and would thus fail.\n", + "
" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "46e4eeaa-03c5-4b3e-b5ca-f87816907ffb", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-05-05 11:49:01,014 [INFO] Created log file\n", + "2024-05-05 11:49:01,122 [INFO] Building cells.\n", + "2024-05-05 11:49:02,108 [INFO] Building recurrent connections\n", + "2024-05-05 11:49:02,125 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n", + "2024-05-05 11:49:02,127 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-05-05 11:49:02,128 [INFO] Block save every 5000 steps\n", + "2024-05-05 11:49:03,210 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-05-05 11:49:04,225 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-05-05 11:49:05,562 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-05-05 11:49:06,625 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-05-05 11:49:06,635 [INFO] Simulation completed in 4.51 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.current_clamp_apic.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "6b89f09d-781b-412d-8331-d13f6d0842e0", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", 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" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "_ = plot_traces(config_file='config.current_clamp_apic.json', node_ids=range(5), report_name='membrane_potential')" + ] + }, + { + "cell_type": "markdown", + "id": "ef02573a-673e-413b-acaa-59674e22c18b", + "metadata": {}, + "source": [ + "### Adding multiple Clamps\n", + "\n", + "If you need to add multiple current-clamps in sequence or in parallel to you simulation you can easily do it by adding multiple sections to the \"inputs\" sections:\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp_1\": {\n", + " \"input_type\": \"current_clamp\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"node_id\": [0]\n", + " },\n", + " \"amp\": 0.1500,\n", + " \"delay\": 500.0,\n", + " \"section_name\": \"soma\"\n", + " },\n", + " \"current_clamp_2\": {\n", + " \"input_type\": \"current_clamp\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"node_id\": [0]\n", + " },\n", + " \"amp\": 0.1750,\n", + " \"delay\": 1500.0,\n", + " \"duration\": 500.0,\n", + " \"section_name\": \"soma\"\n", + " },\n", + " \"current_clamp_3\": {\n", + " \"input_type\": \"current_clamp\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"node_id\": [0]\n", + " },\n", + " \"amp\": 0.2000,\n", + " \"delay\": 2500.0,\n", + " \"duration\": 500.0,\n", + " \"section_name\": \"soma\"\n", + " }\n", + "}\n", + "```\n", + "\n", + "#### As a list\n", + "\n", + "However if you are applying clamps to the same **node_set** and **section** it is also possible to set **amp**, **duration**, and **delay** as a list. The main difference is that we must set the **module** to value `list`.\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp_list\": {\n", + " \"input_type\": \"current_clamp\",\n", + " \"module\": \"list\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"amp\": [0.1500, 0.1750, 0.2000],\n", + " \"delay\": [500.0, 1500.0, 2500.0],\n", + " \"duration\": [500.0, 500.0, 500.0]\n", + " \"section_name\": \"soma\"\n", + " }\n", + "}\n", + "```\n" + ] + }, + { + "cell_type": "markdown", + "id": "601677ed-863b-4fbb-bad6-245dd0dec2e9", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 2. Example: Complex current-clamp waveforms " + ] + }, + { + "cell_type": "markdown", + "id": "04a33110-4561-4fe8-a07e-6e62a22e2833", + "metadata": {}, + "source": [ + "In the above example we inserted a current clamp as single or a series of step-currents. However you may want to use another input form like a wave or a gradual current which, if needed to implement in the above, would be very infeasbile. Luckily, BMTK also allows us to save our current function into a csv file which can contain hundreds to thousand of individual points differentiating how the current will change at each step of time.\n", + "\n", + "Implementing this is done in much the same way as in the example above with two exceptions:\n", + "1. The **module** should be set to value `csv`.\n", + "2. Instead of specifying the **amp**, **delay**, or **duration**; instead you specify the **file** path to the generated csv.\n", + "\n", + "For example to have the sequences of current clamps in the above example we would add the following to our configuration file:\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp_series\": {\n", + " \"input_type\": \"csv\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"file\": \"$INPUT_DIR/iclamp_inputs_series.csv\",\n", + " \"section_name\": \"soma\"\n", + " }\n", + "}\n", + "```\n", + "\n", + "Where our file has the following format, each row specifying the time **time-stamp**, where 0 represents beginning of simulation, at at which the **amp** value is changes" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "1c2ad04a-3fb5-4a60-a9c4-1b422bec6ae9", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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" + ], + "text/plain": [ + " timestamps amps\n", + "0 800.0 0.150\n", + "1 1300.0 0.000\n", + "2 1800.0 0.175\n", + "3 2300.0 0.000\n", + "4 2800.0 0.200\n", + "5 3300.0 0.000" + ] + }, + "execution_count": 3, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "pd.read_csv('inputs/iclamp_inputs_series.csv', sep=' ')" + ] + }, + { + "cell_type": "markdown", + "id": "f98fbb94-da36-404d-85cc-9a83f9154ff5", + "metadata": {}, + "source": [ + "Also note that unlike with the above example there is no **delay**, thus to turn off current between blocks we must reset amp to 0.0" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "0b86ea3a-23bf-40dd-ab82-a6b8063f98a4", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-05-05 12:32:49,833 [INFO] Created log file\n", + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-05-05 12:32:49,851 [INFO] Building cells.\n", + "2024-05-05 12:32:50,585 [INFO] Building recurrent connections\n", + "2024-05-05 12:32:50,609 [INFO] Running simulation for 3000.000 ms with the time step 0.100 ms\n", + "2024-05-05 12:32:50,614 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-05-05 12:32:50,615 [INFO] Block save every 5000 steps\n", + "2024-05-05 12:32:52,358 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-05-05 12:32:54,016 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-05-05 12:32:55,620 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-05-05 12:32:57,227 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-05-05 12:32:58,778 [INFO] step:25000 t_sim:2500.00 ms\n", + "2024-05-05 12:33:00,376 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-05-05 12:33:00,394 [INFO] Simulation completed in 9.785 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.csv_series.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "id": "f92332a6-7e16-448a-a5ba-40f46bbce18c", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "_ = plot_traces(config_file='config.csv_series.json', node_ids=range(5), report_name='membrane_potential')" + ] + }, + { + "cell_type": "markdown", + "id": "f8b062be-38f7-4cba-a5b0-211b126836a6", + "metadata": {}, + "source": [ + "For a more complex example, we will use numpy to create current-clamp trace that has a damped wave. We'll save it to file *inputs/iclamp_damped_wave.csv*" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "3db0e427-b833-4437-81da-28567a27b9e4", + "metadata": {}, + "outputs": [], + "source": [ + "import pandas as pd\n", + "import numpy as np\n", + "\n", + "start_time = 0.0\n", + "stop_time=3000.0\n", + "dt=0.1\n", + "\n", + "times_secs = np.arange(start_time, stop_time, step=dt*200)/1000\n", + "amps_na = np.sin(times_secs*3*np.pi)*np.exp(-times_secs)\n", + "pd.DataFrame({\n", + " 'timestamps': np.round(times_secs*1000, decimals=1),\n", + " 'amps': amps_na*.1\n", + "}).to_csv('inputs/iclamp_damped_wave.csv', sep=' ', index=False)\n" + ] + }, + { + "cell_type": "markdown", + "id": "35c329cc-a5f7-4c79-be44-710db2de0a48", + "metadata": {}, + "source": [ + "Then modify the config as such\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp_wave\": {\n", + " \"input_type\": \"csv\",\n", + " \"module\": \"IClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"file\": \"$INPUT_DIR/iclamp_damped_wave.csv\",\n", + " \"section_name\": \"soma\"\n", + " }\n", + "}\n", + "```\n", + "\n", + "Then run the simulation and plot the membrane potential to see that indeed, the cell is acting as we would expect when injecting a damped wave into the soma" + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "id": "6691c13a-e60b-472a-a47d-faddf35ad1b0", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-05-05 13:05:33,058 [INFO] Created log file\n", + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-05-05 13:05:33,072 [INFO] Building cells.\n", + "2024-05-05 13:05:34,069 [INFO] Building recurrent connections\n", + "2024-05-05 13:05:34,085 [WARNING] IClampMod: Stimulus of ./inputs/iclamp_damped_wave.csv does not end with a 0.0, attempting to set turn off at time 3000.0.\n", + "2024-05-05 13:05:34,093 [INFO] Running simulation for 3000.000 ms with the time step 0.100 ms\n", + "2024-05-05 13:05:34,098 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-05-05 13:05:34,099 [INFO] Block save every 5000 steps\n", + "2024-05-05 13:05:35,856 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-05-05 13:05:37,875 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-05-05 13:05:39,645 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-05-05 13:05:41,639 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-05-05 13:05:43,769 [INFO] step:25000 t_sim:2500.00 ms\n", + "2024-05-05 13:05:45,737 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-05-05 13:05:45,757 [INFO] Simulation completed in 11.66 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.csv_wave.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "4177b173-1914-417d-bd63-b50f913a73b7", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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4t+QLIURmZqZQKpVi8eLFTstLUn9xP8vHjx8X7dq1EzqdTgBwqm/06NEiIiLCaYgGIm8kE4KT0RCRdzp79ixq1KiBadOmlfgsDeUbNGgQTpw4gR07dkjSvslkQvXq1TFmzBi8/PLLktRAVFzsU0REVIaNHz8ee/fuLfEUK66yYMECqFQqj0xKS3S/2KeIiDzOZrPdtcO1N962XxpFRETAaDRK1v7QoUMZiKjUYCgiIo9LT08vsrPxrcaPH+80qSgRkbuxTxEReZzRaMTOnTvvuE3NmjXv+Q44IqJ7wVBEREREBHa0JiIiIgJQTvoU2e12XLhwAX5+fpyMkIiIqJQQQuDGjRuoUqUK5HL3n8cpF6HowoULJZq4kIiIiLxHeno6qlWr5vZ2ykUo8vPzA5B/UO9lqHsiIiLyvJycHISHhzu+x92tXISigktm/v7+DEVERESljKe6vrCjNREREREYioiIiIgAMBQRERERASgnfYqIiIhKK5vNBovFInUZbqFSqaBQKKQuw4GhiIiIyAsJIZCRkYHr169LXYpbBQYGIiwszCvGEWQoIiIi8kIFgSgkJAR6vd4rQoMrCSGQl5eHrKwsAEDlypUlroihiIiIyOvYbDZHIKpYsaLU5biNTqcDAGRlZSEkJETyS2nsaE1ERORlCvoQ6fV6iStxv4L36A39phiKiIiIvFRZu2RWFG96jwxFRERERGAoIiIiIgLAUEREREQu9tlnn6F69erQarWIjY3Fnj17pC6pWBiKPOTG9eu4UcbHmiAiIvruu+8wcuRIjB8/Hvv370eTJk2QkJDguPXemzEUeUBuTja+GbUN347cDLPRKHU5REREbjN9+nQMHjwYAwYMQHR0NObMmQO9Xo/58+dLXdpdcZwiDzi6+3dYVX4AgAtnT6F6vYYSV0RERKWJEAIGi02StnUqRbHvEDObzUhKSsLYsWMdy+RyOeLj45GYmOiuEl2GoYiIiMjLGSw2RL+9TpK2j05KgF5dvLhw+fJl2Gw2hIaGOi0PDQ3F8ePH3VGeS/HyGRERERF4psjj7Da71CUQEVEpo1MpcHRSgmRtF1elSpWgUCiQmZnptDwzMxNhYWGuLs3lGIo8QC73ntE6iYio9JHJZMW+hCUltVqNmJgYbNq0Cb169QIA2O12bNq0CcOHD5e2uGLw/iNMREREpcbIkSPRr18/tGjRAi1btsTHH3+M3NxcDBgwQOrS7oqhiIiIiFzmySefxKVLl/D2228jIyMDTZs2xdq1awt1vvZGDEUeIJP9cz3WbpfmlkoiIiJPGT58eKm4XPZvvPuMiIiICAxFRERERAC8IBRNmTIFDzzwAPz8/BASEoJevXohNTXVaRuj0Yhhw4ahYsWK8PX1Re/evQvd7ldq8PIZERGRV5I8FG3btg3Dhg3DH3/8gQ0bNsBisaBLly7Izc11bDNixAj8+uuv+P7777Ft2zZcuHABjz76qIRVl4xcLvlhJiIioruQvKP12rVrnZ4vXLgQISEhSEpKQrt27ZCdnY158+bhm2++QadOnQAACxYsQP369fHHH3/gP//5jxRll4jdbocjf8qLPwgWEREReY7XncLIzs4GAFSoUAEAkJSUBIvFgvj4eMc29erVQ0RExG0nlzOZTMjJyXF6EBEREd2JV4Uiu92OV155Ba1bt0bDhvkzyWdkZECtViMwMNBp29DQUGRkZBS5nylTpiAgIMDxCA8Pd3fpd+R0+Yx9ioiIiLySV4WiYcOGISUlBcuWLbuv/YwdOxbZ2dmOR3p6uosqJCIiorJK8j5FBYYPH45Vq1Zh+/btqFatmmN5WFgYzGYzrl+/7nS26E6Ty2k0Gmg0GneXTERERGWI5GeKhBAYPnw4VqxYgc2bN6NGjRpO62NiYqBSqbBp0ybHstTUVKSlpSEuLs7T5d43u80udQlERERUBMlD0bBhw7BkyRJ888038PPzQ0ZGBjIyMmAwGAAAAQEBGDRoEEaOHIktW7YgKSkJAwYMQFxcXKm48wwAZArJDzMREZFHbN++HT169ECVKlUgk8nw888/S11SsUn+bf35558jOzsbHTp0QOXKlR2P7777zrHNjBkz8PDDD6N3795o164dwsLC8NNPP0lYNRERERUlNzcXTZo0wWeffSZ1KSUmeZ8iIcRdt9Fqtfjss89K5QEmIiIqT7p164Zu3bpJXcY9kTwUlQcyuQxAfvizFyMEEhERORECsORJ07ZKD8hk0rTtYQxFRERE3s6SB7xXRZq237gAqH2kadvDJO9TVN7Iy0naJiIiKm14psgTZHIAHMmaiIjukUqff8ZGqrbLCYYiTxD/jE3EPkVERFRiMlm5uYQlJYYiIiIicpmbN2/i1KlTjudnzpxBcnIyKlSogIiICAkruzuGIiIiInKZffv2oWPHjo7nI0eOBAD069cPCxculKiq4mEo8oRb+hQJm1XaWoiIiNyoQ4cOxRqD0Bvx7jMiIiIiMBQRERERAWAo8gi5TCF1CURERHQXDEUeZrOVzuusREREZR1DEREREREYioiIiIgAMBR5hELB+c6IiIi8HUMRERERERiKiIiIiAAwFBEREREBYCjyOLuwSV0CERGRW0yZMgUPPPAA/Pz8EBISgl69eiE1NVXqsoqNoYiIiIhcYtu2bRg2bBj++OMPbNiwARaLBV26dEFubq7UpRULJ4T1AJlCCcAkdRlERERutXbtWqfnCxcuREhICJKSktCuXTuJqio+hiJPE3apKyAiolJGCAGD1SBJ2zqlDjLZvQ0tk52dDQCoUKGCK0tyG4YiIiIiL2ewGhD7Tawkbe9+Zjf0Kn2JX2e32/HKK6+gdevWaNiwoRsqcz2GIiIiInK5YcOGISUlBTt37pS6lGJjKPIA+T2ediQiIgLyL2Htfma3ZG2X1PDhw7Fq1Sps374d1apVc0NV7sFQRERE5OVkMtk9XcLyNCEE/u///g8rVqzA1q1bUaNGDalLKhGGIiIiInKJYcOG4ZtvvsHKlSvh5+eHjIwMAEBAQAB0upKfcfI0jlPkAXIFDzMREZV9n3/+ObKzs9GhQwdUrlzZ8fjuu++kLq1YeKaIiIiIXEIIIXUJ94WnMDxM2Ev3B4aIiKisYijyALuNAzYSERF5O4YiIiIiIjAUeZzgWSMiIiKvxFBEREREBIYiIiIiIgAMRUREREQAGIqIiIiIAJSiUPTZZ5+hevXq0Gq1iI2NxZ49e6QuiYiIiMqQUhGKvvvuO4wcORLjx4/H/v370aRJEyQkJCArK0vq0oiIiKiMKBWhaPr06Rg8eDAGDBiA6OhozJkzB3q9HvPnz5e6tBKz23lLPhERlU2ff/45GjduDH9/f/j7+yMuLg5r1qyRuqxi8/pQZDabkZSUhPj4eMcyuVyO+Ph4JCYmFvkak8mEnJwcpwcRERG5V7Vq1TB16lQkJSVh37596NSpE3r27IkjR45IXVqxeH0ounz5Mmw2G0JDQ52Wh4aGIiMjo8jXTJkyBQEBAY5HeHi4J0olIiIq13r06IGHHnoIderUQd26dTF58mT4+vrijz/+kLq0YlFKXYA7jB07FiNHjnQ8z8nJYTAiIqJSSwgBYTBI0rZMp4NMJivx62w2G77//nvk5uYiLi7ODZW5nteHokqVKkGhUCAzM9NpeWZmJsLCwop8jUajgUaj8UR5xWIXwvFvIWwSVkJERKWRMBiQ2jxGkraj9idBptcXe/vDhw8jLi4ORqMRvr6+WLFiBaKjo91Yoet4/eUztVqNmJgYbNq0ybHMbrdj06ZNpSZ5EhERlRdRUVFITk7G7t278eKLL6Jfv344evSo1GUVi9efKQKAkSNHol+/fmjRogVatmyJjz/+GLm5uRgwYIDUpREREbmdTKdD1P4kydouCbVajdq1awMAYmJisHfvXsycORNz5851R3kuVSpC0ZNPPolLly7h7bffRkZGBpo2bYq1a9cW6nxNRERUFslkshJdwvImdrsdJpNJ6jKKpVSEIgAYPnw4hg8fLnUZREREdBtjx45Ft27dEBERgRs3buCbb77B1q1bsW7dOqlLK5ZSE4qIiIjIu2VlZaFv3764ePEiAgIC0LhxY6xbtw4PPvig1KUVC0MRERERucS8efOkLuG+eP3dZ2WN3S7uvhERERF5HEMRERERERiKiIiIiAAwFBEREREBYCjyOCHsUpdARERERWAo8jC7jaGIiIjIGzEUeYCwWaUugYiIiO6CocjDhJ1nioiIiLwRQxERERERGIqIiIiIADAUERERkZtMnToVMpkMr7zyitSlFAtDkadxmg8iIioH9u7di7lz56Jx48ZSl1JsDEUeZmdHayIiKuNu3ryJPn364Msvv0RQUJDU5RSbUuoCiIiI6M6EELCapfmjWqmWQyaTleg1w4YNQ/fu3REfH493333XTZW5HkORhwnwTBEREZWM1WzHFy9vk6TtITPbQ6VRFHv7ZcuWYf/+/di7d68bq3IPhiIiIiJyifT0dLz88svYsGEDtFqt1OWUGEORhwkbO1oTEVHJKNVyDJnZXrK2iyspKQlZWVlo3ry5Y5nNZsP27dvx6aefwmQyQaEo/lknT2Mo8jABm9QlEBFRKSOTyUp0CUsqnTt3xuHDh52WDRgwAPXq1cPo0aO9OhABDEVERETkIn5+fmjYsKHTMh8fH1SsWLHQcm/EW/I9wHbLJTPBcYqIiIi8Es8UERERkdts3bpV6hKKjWeKPIxnioiIiLwTQxERERERGIo8jtN8EBEReSeGIk8TvHxGRETkjRiKiIiIvJQoB39Ie9N7ZCjyMMHLZ0REdBcqlQoAkJeXJ3El7lfwHgves5R4Sz4REZGXUSgUCAwMRFZWFgBAr9eXeKZ6byeEQF5eHrKyshAYGOgVo10zFHmYF50lJCIiLxYWFgYAjmBUVgUGBjreq9QYioiIiLyQTCZD5cqVERISAovFInU5bqFSqbziDFEBhiIPE3ZOCEtERMWnUCi8KjiUZexo7QF28U8Q8qZe9kRERPQPhiIiIiIiMBR5HOc+IyIi8k4MRURERESQMBSdPXsWgwYNQo0aNaDT6VCrVi2MHz8eZrPZabtDhw6hbdu20Gq1CA8PxwcffCBRxa7BPkVERETeSbK7z44fPw673Y65c+eidu3aSElJweDBg5Gbm4sPP/wQAJCTk4MuXbogPj4ec+bMweHDhzFw4EAEBgZiyJAhUpV+XziiNRERkXeSLBR17doVXbt2dTyvWbMmUlNT8fnnnztC0dKlS2E2mzF//nyo1Wo0aNAAycnJmD59eukKRYJBiIiIyNt5VZ+i7OxsVKhQwfE8MTER7dq1g1qtdixLSEhAamoqrl27dtv9mEwm5OTkOD28BS+fEREReSevCUWnTp3CrFmz8MILLziWZWRkIDQ01Gm7gucZGRm33deUKVMQEBDgeISHh7unaCIiIiozXB6KxowZA5lMdsfH8ePHnV5z/vx5dO3aFY8//jgGDx583zWMHTsW2dnZjkd6evp979NVeKaIiIjIO7m8T9GoUaPQv3//O25Ts2ZNx78vXLiAjh07olWrVvjiiy+ctgsLC0NmZqbTsoLnd5o8TqPRQKPRlLByIiIiKs9cHoqCg4MRHBxcrG3Pnz+Pjh07IiYmBgsWLIBc7nziKi4uDuPGjYPFYoFKpQIAbNiwAVFRUQgKCnJ16W7jNGAjzxQRERF5Jcn6FJ0/fx4dOnRAREQEPvzwQ1y6dAkZGRlOfYWeeeYZqNVqDBo0CEeOHMF3332HmTNnYuTIkVKVfd94+YyIiMg7SXZL/oYNG3Dq1CmcOnUK1apVc1pXEBwCAgKwfv16DBs2DDExMahUqRLefvvt0nU7PhEREZUKkoWi/v3737XvEQA0btwYO3bscH9BHsK5z4iIiLyT19yST0RERCQlhiJP4+jWREREXomhyAOEjUGIiIjI2zEUeRj7FBEREXknhiIPE2AoIiIi8kYMRURERERgKPI49rMmIiLyTgxFHmC3MwkRERF5O4YiT+M0H0RERF6JocjTGIqIiIi8EkMRERERERiKPI635BMREXknhiIPEMImdQlERER0FwxFHibYp4iIiMgrMRQRERERgaHI4zj3GRERkXdiKPI0ZiIiIiKvxFBEREREBIYij7DfcslMcPIzIiIir8RQRERERASGIs/jLflEREReiaHIw5iJiIiIvBNDEREREREYijzCqXM1TxURERF5JYYiIiIiIjAUeR5PFBEREXklhiIiIiIiMBR5nGCfIiIiIq/EUORxDEVERETeiKHIA+w2Tu1BRETk7RiKPIxXz4iIiLwTQxERERERGIo8z85TRURERN6IoYiIiIgIDEUeIez/dLQWvPuMiIjIKzEUeRozERERkVdiKCIiIiKCl4Qik8mEpk2bQiaTITk52WndoUOH0LZtW2i1WoSHh+ODDz6QpkhX4T35REREXskrQtHrr7+OKlWqFFqek5ODLl26IDIyEklJSZg2bRomTJiAL774QoIqiYiIqCxTSl3AmjVrsH79evz4449Ys2aN07qlS5fCbDZj/vz5UKvVaNCgAZKTkzF9+nQMGTJEoorvwS234fNEERERkXeS9ExRZmYmBg8ejMWLF0Ov1xdan5iYiHbt2kGtVjuWJSQkIDU1FdeuXbvtfk0mE3JycpweRERERHciWSgSQqB///4YOnQoWrRoUeQ2GRkZCA0NdVpW8DwjI+O2+54yZQoCAgIcj/DwcNcVTkRERGWSy0PRmDFjIJPJ7vg4fvw4Zs2ahRs3bmDs2LGuLgFjx45Fdna245Genu7yNu4Zr58RERF5JZf3KRo1ahT69+9/x21q1qyJzZs3IzExERqNxmldixYt0KdPHyxatAhhYWHIzMx0Wl/wPCws7Lb712g0hfZLREREdCcuD0XBwcEIDg6+63affPIJ3n33XcfzCxcuICEhAd999x1iY2MBAHFxcRg3bhwsFgtUKhUAYMOGDYiKikJQUJCrS/cIwTNFREREXkmyu88iIiKcnvv6+gIAatWqhWrVqgEAnnnmGUycOBGDBg3C6NGjkZKSgpkzZ2LGjBker/d+2G+Z5oOIiIi8k+S35N9JQEAA1q9fj2HDhiEmJgaVKlXC22+/Xbpux/83nigiIiLySl4TiqpXr17kpaXGjRtjx44dElTkHrx6RkRE5J28YkRrIiIiIqkxFHkcTxURERF5I4YiDxBgR2siIiJvx1DkaexURERE5JUYijyNmYiIiMgrMRR5GDMRERGRd2Io8jR2LyIiIvJKDEUeIGz/nB8SPFdERETklRiKPEDA9s+/7QxFRERE3oihyAOcghAzERERkVdiKPIAm/WfM0UMRURERN6JocgTbhmbiJfPiIiIvBNDkQfYbbzljIiIyNsxFHmAsPPyGRERkbdjKPIApzNFDEVEREReiaHIA+y2W27J59xnREREXomhyAOcgpCQSVcIERER3RZDkQcIOy+fEREReTuGIg+49UwRMxEREZF3YijyAGHj3WdERETejqHIAzjNBxERkfdjKPKAW2/JZyYiIiLyTgxFHmA1mx3/ljEVEREReSWGIg+wma3/POEt+URERF6JocgD7JZbBm+UsA4iIiK6PYYiD7BbbjlTxLlhiYiIvBJDkQfYLExCRERE3o6hyAOEldN8EBEReTuGIg+wW259xlBERETkjRiKPECYeJiJiIi8Hb+tPUDYVLc84ZkiIiIib8RQ5AEyu0bqEoiIiOguGIo8QNj9//k3zxQRERF5JYYiD7ArKjj+zUhERETknRiK3Cw3JxsWVdA/CzikNRERkVdiKHKz31d8DyG/paM1zxURERF5JYYiN7uY/KfTc8FQRERE5JUkD0W//fYbYmNjodPpEBQUhF69ejmtT0tLQ/fu3aHX6xESEoLXXnsNVqu16J15IevVAKfnMna0JiIi8kpKKRv/8ccfMXjwYLz33nvo1KkTrFYrUlJSHOttNhu6d++OsLAw7Nq1CxcvXkTfvn2hUqnw3nvvSVh58Rhyc2FV1gMAaA2nYNTVlrgiIiIiuh3JQpHVasXLL7+MadOmYdCgQY7l0dHRjn+vX78eR48excaNGxEaGoqmTZvinXfewejRozFhwgSo1WopSi+2X2bMgFXVCgprHqA8D6A2L58RERF5Kckun+3fvx/nz5+HXC5Hs2bNULlyZXTr1s3pTFFiYiIaNWqE0NBQx7KEhATk5OTgyJEjt923yWRCTk6O00MKN0/kXzpT2/ZDJrcD4OUzIiIibyVZKPrzz/wOyBMmTMCbb76JVatWISgoCB06dMDVq1cBABkZGU6BCIDjeUZGxm33PWXKFAQEBDge4eHhbnoXt7d52WIY9Q0AYUedHvXguBefmYiIiMgruTwUjRkzBjKZ7I6P48ePw27PP3Mybtw49O7dGzExMViwYAFkMhm+//77+6ph7NixyM7OdjzS09Nd8dZK5OzacwAAreEI2j76hCMMcURrIiIi7+TyPkWjRo1C//7977hNzZo1cfHiRQDOfYg0Gg1q1qyJtLQ0AEBYWBj27Nnj9NrMzEzHutvRaDTQaKSbb+zUwX0wqWIAAAENDX8vzT9TJOOpIiIiIq/k8lAUHByM4ODgu24XExMDjUaD1NRUtGnTBgBgsVhw9uxZREZGAgDi4uIwefJkZGVlISQkBACwYcMG+Pv7O4Upb7Nj9grYFZ2hMfyFXiNG5S/8+5wczxQRERF5J8nuPvP398fQoUMxfvx4hIeHIzIyEtOmTQMAPP744wCALl26IDo6Gs899xw++OADZGRk4M0338SwYcMkPRN0J1np52C2tgRUgLpSKpQqldN6mYyhiIiIyBtJOk7RtGnToFQq8dxzz8FgMCA2NhabN29GUFD+XGEKhQKrVq3Ciy++iLi4OPj4+KBfv36YNGmSlGXf0W9T58KqiofadBmPTH3tnxVyAdgBIRTSFUdERES3JWkoUqlU+PDDD/Hhhx/edpvIyEisXr3ag1Xdu+wrl2ExxABqQOV7AH6BTzjWyRX5oUjiQ05ERES3Ifk0H2XJz+9Mh0UdBJX5OnqMG+60Tq4u6FSkKuKVREREJDWGIhfJzcmGObsxAECl2YeKYVWd1jtCERiKiIiIvBFDkYv8NHkazJoQKCw3kfD6gELrlZr8y2ZC5t1TkxAREZVXDEUuYLVYYMqoCwBQy/egSo06hbZR6v4OQzKeKSIiIvJGDEUu8MN7U2DSVYPcZkS74b2K3Eal1wLgmSIiIiJvxVDkArl/5o+urbHtQe0mLYrcxq9SRQCATaH3WF1ERERUfAxF9+nXz2bCqKsNmd2C5n3b3Ha7ms2aAwBsSj2y0s95qjwiIiIqJoai+3Rpb/7cZhpTMpp2iL/tdtVqRUFuMwMATuzfc9vtiIiISBoMRffh1OEDMKmaAgAqNr9zXyGlSgWl5RoA4EJKqmP5t+MnYl6/D5C8daPb6iQiIqK74/DK92HH3O9hV8RDY/wLD780/K7bK8R5AKG4edYIQ24uvn1lKgyKjoAO2LvoMvZ/Mw6B9QLR7YWXoPPxcf8bICIiIgeeKbpHZqMR1tz8wRqVvscLTfxaFLn/FQCAQdEJ80ftzg9EBfvTVIJB3hkXT8Tg2+Ez3FM0ERER3RZD0T368b2p+YM1WvPQZVThwRqL0vl//aEyX3M8l9tM8PPdhLZ9FfBRboTGeBEAYJE3wqUL6W6pm4iIiIrGUHSP8s7m34avFnuLHKyxKOF1otB2aBj0so3QYRNin1ag74eT0bhVe/T/9D08/lECVKarsKr88Mub891ZPhEREf0L+xTdg83ffg2jvi4gbGj0VGyJXlu/ZWvUb9m6yHUBFSvBL+IIrma2hVHdFt+8NR7PvDPRFSUTERHRXfBM0T04u/E0AEBrOIoHHnzIpft+euJ46Ew7AQDZGXFYOYv9i4iIiDyBoaiEDLm5sIomAABt+BW3tPHkzJehzTsCu0KNzAORuHH9ulvaISIion8wFJXQui/mwKIOhMKah4deecktbfj4B6DbxK6Q2W2wqAORuPIHt7RDRERE/2AoKqGrR7IBACpLKoKCw9zWTpUadaAx5g/y+NdOTgtCRETkbgxFJZB95TKs9qYAAFXIVbe3pwr9+xZ9W3NcyTjv9vaIiIjKM4aiElg5dQYs6kAozdnoOWaE29vrOWYEVOZrsKoD8OvkT93eHhERUXnGUFQC5qyaAACVIgkBFSu5vb2AipWg0iXlt30zhgM6EhERuRFDUTFtWroQJl0NyOw2tBjwoMfa7T7mBajM12DRVMCvE+Z4rF0iIqLyhqGomM5t/hMAoDEeReNW7T3Wbkh4JLQV9wMATKINkrdu9FjbRERE5QlDUTGYjUZY7fmTv2qqXvZ4+8+8NwEaQxrsCg0Ortjs8faJiIjKA4aiYlg993NYNBWgsBqQ8H9DPN6+UqWCXJ4GALDm6DzePhERUXnAUFQMVw/nz2yvshxHcJVwSWrQhdsBADbUh9VikaQGIiKisoyh6C6sFgtstvoAAFWw+8cmup34oYMgt5lg0VTAqs9mSVYHERFRWcVQdBerPv0EZk0o5DYT4of1l6yO4CrhUJsPAgCuJJskq4OIiKisYii6iysHzQAAtfkgqtSoI2ktgY3yf1xmVRNcOHNS0lqIiIjKGoaiO8g4dxoWZf5dZ4ENpT9UPV8eAbUpC3aFFuunL5C6HCIiojJF+m96L7Z+1nzYlDqoTZfR4//+J3U5UKpUUPocAgBYbjZBbk62xBURERGVHQxFd2DJqgIAUCgPQ63VSlxNvm6vDYLSchNmTTBWTJ4mdTnlgtloxB9rfsHeDaulLoWIiNxIKXUB3ipp0xoYdVEAgKiHm0pbzC3CImtBJVsEKzrAdLGC1OWUWT/P+AhXk22wy6rArA6GkPsCAJKXfgMFzkBVKQ//HTvCI3PgERGRZzAU3cahH3cAsnhoDafQuqfnB2y8E301BQwZgJCHSF1KmfPHml9w5NuTMOqbAUWMk2nWhgEIg+Em8P3IX1C1rRndnh/q8TqJiMj1ePnsNuw38wdplPmclbaQIkR36QAAMGmrICVxp7TFlCGLXx2H5B8V+YFI2KE17kbFKjvRtNs1DPwoFgkv+iMoeBt05h2Q20ww6arjz311Ma/vx9i58nupyyciovvEM0VFMBuNMGtqAACqt60ncTWFNW7VHnvmLoJJF44DP65Bw7g2UpdU6i0c/gZyrfGAEtAaTqNaByBhwFinbWo3aYHaTVoAyL+8mvz1fhi1TWHUN8axny+hWlQKqtdrKEH1RETkCjxTVIRfZ30Cu0INmd2C2O49pS6nSHLVaQCAOYt9Wu7Xwv8bmx+IAGjNO/DM7GeQMGDwHV8T07kbBi16FfXa/QWlORtmTTA2TtmDQ79v8UTJRETkBpKGohMnTqBnz56oVKkS/P390aZNG2zZ4vylkpaWhu7du0Ov1yMkJASvvfYarFarW+u6ftgPAKA174OPf4Bb27pXVVpVAwCYNfWRfjJV4mpKr0Uvv4Fcy4MAAJ15O/rNfQM6H59iv75zn/4Ij8uAwnITJl11/DHvCtYt+NJd5RIRkRtJGooefvhhWK1WbN68GUlJSWjSpAkefvhhZGRkAABsNhu6d+8Os9mMXbt2YdGiRVi4cCHefvttt9X044cfwKiPgsxuRcNnmritnfsV/9wAqI2ZsCvU2PLF11KXUyotmzQJN42dAOSfIeo7dxyUKlWJ9/PQ4BcR3T0XamMmLOoKOPN7FSRv3ejqcomIyM0kC0WXL1/GyZMnMWbMGDRu3Bh16tTB1KlTkZeXh5SUFADA+vXrcfToUSxZsgRNmzZFt27d8M477+Czzz6D2Wx2S13XD+XfcqS17EHLLg+7pQ1XUKpUUCiPAQAsmbwLraQ2L1uM62kxgEwOrWE3+s19454CUYF2jz6NjqPqQWM8D5tSh6T5B2G1WFxYMRERuZtkoahixYqIiorC119/jdzcXFitVsydOxchISGIiYkBACQmJqJRo0YIDQ11vC4hIQE5OTk4cuSIy2vaufJ7GHX1AQB1etR1+f5dLaJDTQCASVsfpw4fkLia0iM3Jxt/rrHAptRBaziJx6YPvq9AVKB2o2YIbXkdMrsNRn0zfP3SRBdUS0REniJZKJLJZNi4cSMOHDgAPz8/aLVaTJ8+HWvXrkVQUBAAICMjwykQAXA8L7jEVhSTyYScnBynR3GcWHkk/8xB3lG0ffSJe3xnnhP/bH9ojOch5Er8Pp+3hBfXd69/AJOuOhTWPDTrV8OlAzD2eOn/oNdsBgAYFJ2waMQbLts3ERG5l8tD0ZgxYyCTye74OH78OIQQGDZsGEJCQrBjxw7s2bMHvXr1Qo8ePXDx4sX7qmHKlCkICAhwPMLD88ccutNcYWajEVbkT/6qi7x0X+17klx9HABgvVJV4kpKh41LFsJobwcA0PntQvNOXVzeRv9ZU6CzbgUA5OZ2xIoZH7q8DSIicj2Xh6JRo0bh2LFjd3zUrFkTmzdvxqpVq7Bs2TK0bt0azZs3x+zZs6HT6bBo0SIAQFhYGDIzM532X/A8LCzstjWMHTsW2dnZjkd6ejoAYOOC288sv37hV7CoA6GwGtDtfy/e72HwmDpd8zuDG3V1eRfaXeTmZOPsBgEhV0Gbl4I+H7jv8lbfz9+CNu8AhFyBq8n+7F9ERFQKuDwUBQcHo169end8qNVq5OXl5Rcgdy5BLpfDbrcDAOLi4nD48GFkZWU51m/YsAH+/v6Ijo6+bQ0ajQb+/v5ODwDIOW687WuykvIvx6nMaQgKvn3g8jZtH30CKvNVQKbA7p9+krocr7Z89Psw6SKhsOYhZmC0S/oR3Y5SpUKDp+tAbjPDqKuNpa+Pd1tbRETkGpL1KYqLi0NQUBD69euHgwcP4sSJE3jttddw5swZdO/eHQDQpUsXREdH47nnnsPBgwexbt06vPnmmxg2bBg0Gk2J27TK6sOQm1to+c8zZ8Aoy7+kIvNNv783JgGF7SwAIOdknrSFeLFDu7bBZGkNAND57kLTDvFub/M/3f4LjWIHACDvZltsXrbY7W0SEdG9kywUVapUCWvXrsXNmzfRqVMntGjRAjt37sTKlSvRpEn+JSGFQoFVq1ZBoVAgLi4Ozz77LPr27YtJkybdU5sWdSBWzZzptOx40m5kHYyEkCuhzUvGs9PdNwaSuygrXAUA2C01Ja7Ee+37YidsSh00hnN4eqrnfsZPfvA6tHknYFdo8OdaGbLSz3msbSIiKhlJ5z5r0aIF1q1bd8dtIiMjsXr1ape1eSPV+S3v+mQbLLoWUBsvostb3aHWal3Wlqc0fTQeO5cCJl0kDu3ahsat2ktdkldJ2rQGRnVLAEClplc9+jP28Q/AAy9EIXHedZi0VbBq/EIMnM9LaURE3qjczX1mUjdG2omjAIBVcz6FQdcCEHaEPXAZ4XWiJK7u3jRp2wkaQxoA4MD3dw6Z5dHBJXsh5ApoDSfRa8Qoj7ffuHVHBEQcAgCYFC1xYv8ej9dARER3V65CUcG0GL9Oz8C8vnNwLjm/s7bWuB89hr0scXX3R67OnyDWeqWyxJV4l01LF8KgjgMA+EddkayOx8a9AY0hHXaFBjtnrZWsDiIiuj1JL595mlJ3DAL5/W6M+n9GrFYGXpeoItep3rkujm0FTNp6OLF/D+o2byl1SV7h7LpcQK+ANu8QHn9DuoEUlSoVAuqdR9a5cBjUcVg151M8PHS4ZPWURRnnTmPTF4tguiiDsAZByCvCJveDwpYDmciBTH4TUN+Af20/dOz7HCqGcWwvInImE0IIqYtwt5ycHAQEBGD/ru34Y34u7HIlfNSbYMvVQiYUeGjC0wiLrCV1mfftq36LYNKFw1ezEf1mvid1OZJbMeNDXEhtDpndisbds9Gm5+NSl4T5/T6EQdccGuNFPDK1A7+Y75PVYsGqTz/BlWQBs7oh7Ap1sV4nt5mhNqegchsfPDS49IxLRlTeFHx/Z2dnO4bXcadydaaoVoMmsDy+HYAVLbtMkbocl5PrTgAIh/VauNSleIWrB7SAHtCa96JNz3FSlwMAaPNyO2z59AJM2spY+eZcDPzq3u6kJODnGR/hSnIAjLpmQP48zlCbLkMhTkDumwddmB6+wUG4kXkFpmtG2HJlEKYKsClqwKIOhFHXHGeSgK92zoM27Cyeevdtt45dRUTer1ydKfJU0pRK4q8rsP+3AEDY0WmQDvVbtpa6JMmsW/AlTu2uBQgbmj6Ug9Y9e0tdksPSceNx/Up7yOw2/Ocp4ZapRsqyE/v3YMfMbTDq8ieOltvMUFsOICzOHwkDh9w12FgtFqydNxdZiUYYNY0h5Pl/G2rzUlCvd1Wv+qwQlXee/v4uVx2ty7q4Ho9AY/gLkMmxf0X57sx7fnv+/HlaQ4rXfcn1mTwRWsNpCLkCyd/slLqcUmXZxEnY8un5/EAk7NAZE9GqrxqDFo5F9xeGFetMj1KlwsNDh2PgolfRpr8GOutWyOw2GPUNcfA3X8wf/BauZJz3wLshIm/DUFTGyGUXAACmyxIXIqFTB/fBpGwBAPCLKjyCuTfQVssfOd0ki0NKIoPR3ZiNRswf9DauXGwDqzoAamMGIhofwcCF49Ckbad73m/jVu0x8KtJqNfxIrR5qRByFQyKjlgxegMSf/vZdW+AiEoFhqIyRu6XAwAQ5moSVyKd7bN+gV2hhcb4Fx59fbTU5RTpyQlvQWM4B7tCg91zfpe6HK924/p1LHnhUxhUHQAAOtNO9HqvrUuH0ej0dF/0m/c8AgK2QGm5CZMuAgdXqLBoxBuczJeoHGEoKmNqtG8IADBpqpfLKSWOJ+2GSfYfAIC+2mmv7TirVKkQ0iIbEHYYdQ/g+6llr+O/K1y7lIHl/1sIgy7/LkI/300YuOBtBFdx/c0ESpUKz77/Dhr+1wKt4TRsSh1uGuKx6PmPipwzkYjKHoaiMqZ1z8ehMl+DkKuwZVH5m4B015xV+WeJDGl4YvybUpdzR//9v1egNScCAK4fr47sK+X4mmcRMs6dxk+jfoRR3xgyuwVBlRPR98PJbm+3dc/eeGb2M9DZNwHCBqOuJb55cZ5jJHwiKrvK1S355YFSpYLCfgIWxOLm6TJ/Y6GTa5cyYLHHAApAG+a9Z4lu1Xn0I1j3wSmYtaFYMfEj9P+EZ4wA4OzxFGyasgtGfX3IbSZUqnkAj4/13JxxOh8fDPxiMpaOG4/sS61g1DfE+vcOoP4jx7yu435pZ7VYcPLAHpzauw83s67DcsMEYQNkCgAKGZQaJQIjw1CvVWvUbNBY6nKpjGMoKoP0kWYYMwGbLBpWi6VUhANXWPX+LFhVnaEyX0PPsZ6f4+xeVK/XEGrNN7CKeFhz6sNsNJbKSYld6cT+Pdg+8whMutpQWPMQ1ugker0szWjkfSZPxK+zZ+HivqowaSsj5ZebuJ45G92HvCRJPWWB2WjE6rmf4+qRKxDGUFjU1WFT+gBoVPQLbgDXLgNnki5DYf0VKstFyJTnoa0CNOvRpVwPPUKux3GKyqArGeex/K1DsCs0iGx6tNxMJzGv76cw6qOhs23GwC/flbqcYjt1cB82zsqETalDYNAW9JnyjtQlSSb9ZCrWvfs7TLrqUFpuIPw/F7xixOn9m9dj/6LzMOkiobDmIrjeMfR+9XWpyyo1zEYjVs+ZjauHbsCKhrCogwptozJfg9yWA5nIA2BDfu8OBYRMA5uyAqyqov/vVhszIMcZhP4noNz8X1eecERrum8Vw6pCbV4Go64ZsvZlSV2ORyRtWgOjLgoAUPPB+hJXUzK1m7TAdtlbMKAj8jIa4tKFdLd0JPZ2htxcrH9nA0z6aCgtN1G70zV07iN9IAKA5p26oFK1o9gweReMuprIPNEUi18dh+c80MepNEs7cRQbP14Cm6EpzJqmwN+zsCisBqjMxyH3v4LghlUQ1+uRu055c+HMSRzeuhUZh87Ads0HdlSHSVsZZm0YgDCcSwa+6r8UMsUJ1IivhU5P93X326MyiGeKyqivXxuHGzc6Q2O8gOcXPit1OW43b9B4GFXtoc07iUFfvyB1OSWWfjIVa6YcgUUdCJ1tCwZ+Wf7OFs0f8iYM8k6Q20yo1vSES2+5d5VLF9Lxy9jl+VOLAPBRbUD/WewH9m9/rPkFR74/AIu8BWzK/DlYFFYDVJYj0EXkImHYEJfM+3fq4D7s+eFXGNP8nEYnh7BBazwEn1q56PHKy/DxD7jvtkganv7+Zigqo/48cghrZ2ZCyBVo3OUy2j76hNQluc3Z4ylY++E52JQ6BAVvwzPvTJS6pHuyZOxbyL7WETK7Bc165CGuxyNSl+QxC4e/gVxrPAB4/YTGZqMRS156HwZ1W0DY4avd7NX1etKh37dg31e/w6iKhZArAABq40WoA47godEvuvUM6KnDB5C4+EeYM8Jg1Ec7lqtNl6GpeBA9x45AQMVKbmu/vLh0IR1Hd+1A1smzMFzJhd0kYLfIAKsCEDIAgAAgk9shU9ogVwMKvQK+IYEIq1cHDeLawS8wsNjtMRS5QXkMRQAwr+9sGPX1oJdtxIDPy+5/2vOHjoMBnaExXkTfOb1LbUdlq8WCRYO+hFFfD1rDXgxa5J0DT7pawVxwAKC1bEO/OW96/c0BVosFXw9+HwZtKwCADpswcE75vZR26UI6Vr3zOUyWuL87TQPavFT41L6ER19/3eO/k6u//BwZuy7BIm8Kq8oXAKA050ClSEKnUU+ier2GHq2nNLJaLNi2fCn+2nMCthwthC0YVmVlWNX3+R0q7FBZrkNhzQIUV6DwMSCwVkW07PVfVKlRp9DmDEVuUF5D0YKX3kCePR7avCMY9PX/SV2OW+TmZGPpK5tgUQfCz2cT+n5Uur+Yfp09C2mHGkBmt6D1c6r7msKiNNi8bDFSN1WEXaGF1vQ7+n0xxusDUQGrxYKvh77zz0jblm3oWwoCnat9+/YE3PirkaPztMaQhqBGWV7RET3j3GmsnjwfFmsLWNX5l9CUlhtQKfYg7vmHeOfaLawWC3av/gWnNifBej0IVkWd2wYguc0EpeUa5PZsAAbI5GZAZoaQ5ccJGQDYFRBCDQgtINPBLg+ARRUAIb/N74ewQ226BLlIh9zvGkIaVUO7J/tAyOUMRa5WXkPRylkz8NeRJlBabmDQnIfK5H/W3749AVez2kFpuYGnPmhdJk6Pz+v3JYy6WtCafsegBW9JXY7b3Lh+HctfXg6jrja0eSfwxCdPlei0urdY8OIbyBP5l/501q3o+/lbZfJ37d+SNq1B8teHYNQ9AABQma5CE7gfj00c7XV9eG5cv46VUz6CIbP+3x2zAZndAq01ER1GPVpuxz86uGMzDq7cBMslX9jktWHRVHRaL7eZoTadgUyZCUWAGRXqVEb9tm1QvV7De/qMm41GHN+/G3/uSUL2ucuw5aggLBVhU1aDRR1YaHuZ3QLbjT/xv2UvMRS5UnkNRdcuZWDZG8mwK9SoE/cnuvR7XuqSXG5e35kw6htBZ96OgfMnSF2OS/zwwVRk/tkSMrsVDbpcRvvHnpG6JJczG41Y/MJMGHUPQG4zoWkvE+K695K6rHu2YPgbyPu7T5TOuAe9P3q+TAT0opiNRiwdOQlGSzvYFfm3k+nM29Hj3UFef9dkbk42fpz0AcyXasOkiwSQ3wFcbd+HqJ4Ny8XAnDt+Wo5TG5Jhz6sJo66m0zqZ3QqN6Sxk6nQE1PVDp/59ERQc5pG6UhJ34siGLchNN0GYwv4ev0oPgzkXry34L0ORK5XXUAQA8/rOglHfoEz2K0o/mYpfp52DkCtRr20aOvfpL3VJLlPwc9MaTqDfV4PK3JmH+c+/BYOyIyBsqFh5F56a4LnRqt1l4ctvINfYEZApoM1LwaMfPeGxLxRP+X3ljzj+4yUY9XUBAFrDKYTEmtDjpdJ1ed5qseCHKVNw88+aMGmrAPjnzFGLQe3RuHVHiSt0rcRfV+DYr/tgN0fBpHWeLFxjOAe58ix8a6rQ7tmnERZZS6IqnZmNRuxZ9yuObd+LIdOnMRS5UnkORf/0KzqOQV+XrVF4F414AzcN8VCbMjF4wdNSl+NSv6/8EYdW+cCuUJeJvlK3+u7dd3E5/T+ATA5f3Ub0m1F2wvqyiZNw7a+WsCvU0OYdQq8PHnfJredSM+TmYtmrk2G05p8dkttM0Gl34NnpE0t1YDcbjVjx4Ye4eaIijPr8cc5kdgu05gOo2r4iEgYMlrjCe3c8aTcS56+E/ea/zggJG7SGk1AE/IWmvTuhaYd46YosBna0doPyHIo2L1uMY1urQma3oMdr1RFeJ0rqklzCarFg4fNLYdJFlNk7fwruqlOZr+HJae3LxOWYjHOn8cuEA7BoKkBn2oWBC7x70t57sXzyZFw+1wJCroLWcBrtRzZD7SYtpC7rnh3atQ175xyCUd8AAKA1nEDUoxXRpufjElfmOlaLBT9OmYqbpys7BQht3nH41LqMx94YWyrC36mD+/D7wp9gvVIZJm3dfzo1Czu0hpNQVkxH6/6PlqrPI0ORG5TnUGS1WLBgyA8wa0LhH7AZz71feqa/uJMVMz7EhdTmkNvM6Dg0CPViYqUuyeWuZJzHD2/sgVUdAD/fTR6ZId6dDLm5+ObFuTDqm0JlvopeEx9ASHik1GW5xQ/vT8Hlk41hU+qgMV5Ekz5BeODBh6Quq0TMRiO+eXUSjMZWsCn1kNvM0PtsR59ppfvs0N2snDUDl/fZYdQ2AWRyAIDGkA5VUCoSRgzymstLBdJPpmLrl4thzqgEkzb6nwEskX8noML3BJo97v1nhG6H03yQSylVKigUxwCEwnyxgtTluMzVZAWgA9SWJNSLGSd1OW5RMawqVMp9sKIzTFea4NqljFLdR+Xb//sQRn1byOwWVIz+EyHhj0ldkts8NnosNnw9D2e2BsKkrYwD315DTtbCUtPvLXnrRiTNPwajPh5Q5n+5Vm1rRLfny86lztvp+X8jAACJv/2Moz8ehFnxAEy6cJiM4VjxbirU5p+gC7+JhOGuGZX7Xhzb8zv2Ll8NS1YFmDUNYFd0AvT56zTGvyBXn0Bk+9ql5vPmTXimqBxY/eXnOJMUBbnNjEferON1f+mU1M6V3+Pg6iBAJkf99n+V6TmOMs6dxi8TD8KiDoQemzCglF4mLBitG0CZOmN5N3vWr8LBb3Jg1oZBbjPCt8Iur37vVosFS18fD8ON/LNDMrsFOvVWPPX+m9D5+EhdniRO7N+D37/8GVZjY5g1IY7lcpsRavMR6CJuumzaktu5dikDWxYvxvVj2bCba8CkjXScxQIAtSkTCsUxRHSoifhn+7utDinw8pkblPdQlH8J7SeYNcEICNqCZ0v5LOzzn38bBmUHaPOOYtDXZX9W7IIO5XKbGQ26XUO7R0tXp/Jje37Hti+uwabUQ2fZioHzJkldkkcdT9qN3z9JhlGXP1qvt45ldGL/Huz4eI9jigyNIQ2RnW14sO8giSvzDlaLBWu+mI1LSddgRUNY1P+cec+f4PYk5PpLCKgbiLZPPXXPwxNYLRYc2LoBp3bsgeGiDcJUFWZNdcfwBwU0hr8gV51CSIsQdB30gtd9nlyFl8/I5ZQqFRSyYwCCYbrgJ3U598WQmwurvQkAQFM5U+JqPKPPBxOxaNAXMOrr4+TKG2jVw1Jq/gM0G43Y9ele2PSNoTGk4/GZI6UuyePqxcQifFY9fDfiIxhUHWBQdsCi5z9B+1c7oXajZlKXh9ycbPzw1vsw3ngAVn005DYztIodeGLW6143CKOUlCqVY5Jiq8WC3+Z8issHcmBDQ1jUQbAp8weAzDsBfD/hONTmbZDZrwDK65DrTVBoZVBqlZAp5JDJAKvJCpvRBrsJECYVhNUfAkGwKSv9PTVJO0ABx2UxpSUHSstpKAIvo1HPdojpXHbPkEuJZ4rKiYKOyQrLTTw/p1up+VL9tx8//AAZp1pAYc3D4+82KxO3OxfHH2t+wYGfVLArNAiqtA3PvFs6Jr2dN3AijOq2kNmtCG+aWurGs3G1W8cyUpmvIqTROfQaMUqyetYt+BLpW1Uw6SIAAGpjJiLa3yzVt6J7mtloxNov5+DK0SzYc4NhU0YWOTpziQgbNKYMyMQFKPyvI6J1NNr1fqrU/r99P3j5zA0YioDsK5exdGwShFyFJt2ulNrbaecPGg+Dqj20hiQMWvSa1OV41LxB42FUtYfG8Bf6zO7t9X08Fr3yBm4a8+948fPbhL7TSmd/KFf7ZdbHyNxfGWZNMCBs0Nm245Epwzzaif7Qrm3Y+8VOGDWxgEwOhTUXGnUiek38X6nuzO8NrBYL9m9ZhzN7kmHIzIMtVwPYfAGhhZBpkT8zmBwymAFhAmRmyOS5kOmMUAepEFQ9DLE9/ltu/uC7G4YiN2Aoylcwp5aPZiP6zyx9d5FcyTiPH8btg1Xlh8CgLehTyvtGldStfXN8lBvR/1Pv/RluXb4UxzZUhF2hhs62GQO/9N7OxVJIP5mK9e+uhFGXP16M2pgB38iTePzNN9x6NuDUwX3YPmslTLI42BX5M9dr85LRYnDTMj/5MJVOnv7+lt99EyorZJpzAADbtZC7bOmdVr3/Gawqv/wxbsa+KnU5Hle/ZWtotbsAAEZjayT+ukLiiopmyM3F6VXX/x7V+TienVX2Bmi8X+F1otDvqxEICNoCpSX/7rSrmW2xaNA8/Dp7lsvbO3VwH+Y//zY2zsqEQdkRdoUWGsNZhNXeh0Ffj2QgIvobQ1E5EhpTGQBg1tRF9pXLEldTMlaLBZZr9QEAKs3+ctsB9Mn3x0FrOA2bUoejP1xBbk621CU5MRuN+OalT2DU14fcZkaNh/yg1mqlLssrKVUqPDvlHXR+JRI6yzbI7BYY9XWRdqgB5vX9BMsmToIhN/ee92+1WPDzx9Mxv/972PBpFgzKDn8PJnkegUFb0P+r59D71ddd+I6ISj9ePitHzEYjFry0Fla1P4LDd+GJcaXnL/jlkyfjUnoc5DYTEl6pipoNGktdkmQSf/sZB1eoYFPqoJdvxIDZ3nMZrWC4BAg7AitsK3eXOO/HnvWrcHjpERi1MY4xaFSmK1DKU+AfpUPHvs/dtZ9J+slU/P7Nd8g9J2AXdWHWhDrWaYx/QVf1NB5/ayyDKpUavCWf3Eat1UJpPwIr4nDjhELqckrkRqovoAc01r2o2aCb1OVIKq57L5xY/wZumuJhMrdCSuJONIxrI3VZ+Hb8xPxABMBXvxl9pnhPWCsNWnZ5GC27PIyty5fi9NpTsMibw6KpCAvaw/An8N3bR6E2b4JMZOZ3zpXZALkNwqYG7P4Q8iCYNKGArA3w95A2cpsJavMhBDaUoecrI8rl3UtEJcFQVM4E1JfDeAawqBoh+8rlUjHJ6LE9v8OkzR9QLjI+QuJqvMMT743D0pd+gEkXjt2fJ6Fei1hJv/AO7tiMG+mNADWgM+1EvzkMRPeqwxN90OEJ4NKFdKydORfmDD9YFVGwqv1h0lYDUM35Bf/6satNl6EQp6AKzUOH559DeJ3y/UcEUUm4rU/R5MmT0apVK+j1egQGBha5TVpaGrp37w69Xo+QkBC89tprsFqtTtts3boVzZs3h0ajQe3atbFw4UJ3lVwudB/+PyjN2bAp9Vgz6zOpyymWPxb9BiFXQGM4y7l8/qbz8UF4B/Pf/VAaYcmI8ZLVcjxpN/bOOw+LOhBqYyZ6TuEIyK4QXCUcz73/LgYtGo1Bc7ujZW8jgoK3QS/bCJ1tM7SWbdCZd0Bn2wIfzUYEh+/CA4/mYfCCJzBw4Rt47v13EV4nSuq3QVSquO1MkdlsxuOPP464uDjMmzev0HqbzYbu3bsjLCwMu3btwsWLF9G3b1+oVCq8917+X5lnzpxB9+7dMXToUCxduhSbNm3C888/j8qVKyMhIcFdpZdpOh8fqMRhWNEGuWdKR78C+40qgB5Q+JySuhSvkjBgMBbsfQN59njkmTvip+nT8OhIz47ddOrwAez8JBUmXTWozNcR/Yia46u4gVKlwgMPPoQHHnxI6lKIyjS3nSmaOHEiRowYgUaNGhW5fv369Th69CiWLFmCpk2bolu3bnjnnXfw2WefwWw2AwDmzJmDGjVq4KOPPkL9+vUxfPhwPPbYY5gxY4a7yi4XAhrkhyGLKho3rl+Xtpi72LthNYy6/L92I1rXlbga7/PczInQGg5AyBW4dKQO9m5Y7bG2zUYjtk3bCZOuGpTmHNTtakDrnr091j4RkatJdkt+YmIiGjVqhNDQf+6OSEhIQE5ODo4cOeLYJj4+3ul1CQkJSExM9GitZc1DLw6D0nIDNqUP1s39XOpy7ujwt3sAmQLavBO8dFYEpUqF7pN6QWP4C1aVPw4uycKlC+lub9dqsWDpy+/AqG8Auc2M8LiL6PBEH7e3S0TkTpKFooyMDKdABMDxPCMj447b5OTkwGAw3HbfJpMJOTk5Tg/6h87HB0prKgAg5+Ttj6PUzh5PgVn+AABAX6N8TP56L8Iia6HBE/5QWm7CpIvAL2O/gdVicVt7N65fx6LnpyNP1hkAoJXvwEODX3Rbe0REnlKiUDRmzBjIZLI7Po4fP+6uWottypQpCAgIcDzCw8OlLsnrKCtcBwDYLTWlLeQOtsz85u/B5i6i99jRUpfj1eK690KF2imAsMGoewCLXnjPLcEoNycby19eAKPuAUDYoJdvxHOfTnB5O0REUihRKBo1ahSOHTt2x0fNmsX7kg0LC0NmpvNf/wXPw8LC7riNv78/dDrdbfc9duxYZGdnOx7p6e6/nFDaxD7bExA2mHQR+H3lj1KXU0huTjYspvx5oVRBRzjYXDE8PvYN+Gi3AACM6rb4esgUlwajrPRzWPZ/c2HUNYHMbkHFKokYMPs9jn1DRGVGiUJRcHAw6tWrd8eHWq0u1r7i4uJw+PBhZGVlOZZt2LAB/v7+iI6OdmyzadMmp9dt2LABcXFxd9y3RqOBv7+/04Oc1YuJhdaQfzfXibVJEldT2C8ffQyLOhBKyw38943/SV1OqdF/5nvw1WwEhB0GTRt8PeR9mI3G+97v5mWL8ctb2/MnMBV2+AftxFPj33ZBxURE3sNtfYrS0tKQnJyMtLQ02Gw2JCcnIzk5GTdv3gQAdOnSBdHR0Xjuuedw8OBBrFu3Dm+++SaGDRsGjUYDABg6dCj+/PNPvP766zh+/Dhmz56N5cuXY8SIEe4qu1xRBOSfQbPl1ZG4ksLyzuTPbaYSBxAUHCZxNaVLv5nvwc9n89/BqBUWD/kSx5N23/P+vnlrPFI3VYJJWxVKcw5CauzFs+9z+g4iKnvcNvdZ//79sWjRokLLt2zZgg4dOgAAzp07hxdffBFbt26Fj48P+vXrh6lTp0Kp/Gf4pK1bt2LEiBE4evQoqlWrhrfeegv9+/cvUS2c+6xoh3Ztw86FZgi5AlFt0hD/bH+pSwIAXMk4j+/eToGQq1A79jQSBgyWuqRSafHoN3HjWlsIuQoq83X4VTuEx98aV+zLXZu//Rpn1lyDUZ8/rIY2LxUPDI1G41bt3Vk2EZGDp7+/OSFsOTev78cw6htDa96BQfOlGxX5Vl+/Og43bnaGynwNA+f+l31W7sO6BV8ibZsPzNr8s23avGPwrXsNDw0fDr8iRpq3WizY/uMynF17DgbNf/InJhU26MyJeHzG/4p8DRGRu3BCWPIon5rZMGYAVlkzXLuUIfmlKrPRCNPlaEALKNX7oVRxMMD7kTBgMDI6ncbqSV/DqGgFo74+jH8BS1/dCZXlKGTqHAAyAIAw+8KmqA2LuiqgzR+VWpt3CLUeDkaHJ9h/iIjKPp4pKudyc7Kx9JWNsKiDEBS8Dc+8M1HSepaMfgvZ2R2hsOah66vVUb1eQ0nrKUt2/LQcJ35NhVXeGFaV3223k9kt0BhPwaf2FTz1NsMQEUmHZ4rIo3z8A6AUR2BBGxjSpL3t3Ww0wpgZBWgBtWw3qtd7WNJ6ypq2jz6Bto8ChtxcrPtiDq6m5AA2DQA7hExAJrPBt5YKHfo+i5Bwzi1IROUPQxHBr64ShnOAVVkfhtxc6Hx8JKnjh8lTYNK2h8Kahw4jHpekhvJA5+ODXiNGSV0GEZHXkWyaD/IeXV98CUrLTVhV/vjlo+mS1WE4FwIAUIu9qNmgsWR1EBFR+cRQRPALDITKngwAuHlKL0kNib+ugFFfHxB21O/VVJIaiIiofGMoIgBA5db5Z2lM2oY4sX+Px9s/tmYvAEBrPI24Ho94vH0iIiKGIgIAdHt+KDSGNAi5Cju/WOnRti+cOQmLsSUAQOF/zqNtExERFWAoIgd1pZMAAKs5BtcuZXis3bXvfQ2r2h9q0yU8MmGkx9olIiK6FUMROfR8YwRU5muwqAPxy3ufeKTN5K0bYUIrAIAuJAUBFSt5pF0iIqJ/Yygih4CKlaBU7wcAWK/W9Eib+xfuhl2hgdbwJ556lwMFEhGRdBiKyElMn86AsMGoq4nN337t1raSt26EUf0AAKBC0+uc44yIiCTFUEROmrTtBK3hCADg7Lo0t7a1f+k2CLkSWsMpPDLiVbe2RUREdDcMRVSIf7QBAGBUP4CDOza7pY30k6mw2poCAFQVz7qlDSIiopJgKKJCHhn1KrSG0xByFfYt2OGWNta/txwWdRDUpsvo8vIgt7RBRERUEgxFVIhSpYJvrfxb8s2KWOzdsNql+//hg6kwaloDAALrnEJYZC2X7p+IiOheMBRRkXqPHQOt4RTsCjUOLTnssv1eupCOa8dqAAC0xkQ8/sYbLts3ERHR/WAooiIpVSqExpkBAEbdA/h19iyX7PfXSXNg1gRDZb6GB9/gdB5EROQ9GIroth4eOhxaQ/6cZFm7NbBaLPe1v0sX0mGxxAIAtBX2I6Ju9H3XSERE5CoMRXRHTfs2htxmhlFXG9+MnXDP+zHk5uKXMT/AqvKF0nIDj7z5muuKJCIicgGGIrqjmM7doJHl34GWd70V/ljzS4n3YbVY8O2wGTDqm0Bmt6Bi3aPwCwx0caVERET3h6GI7urJD1+H1vAnbEodjizLwpWM8yV6/eKXx8OgbQUIOwIq7sRjo8e6qVIiIqJ7x1BEd+XjH4B6jwZBYc2DUVcTP49eBrPRWKzXrl/0FQzWDgAAvWIz+kx5x42VEhER3TuGIiqW1j17o2Ltw5DZrTDqmmHxC58gNyf7tttbLRYsfnUc/twe8vdUHifw3MyJHqyYiIioZBiKqNgeHzMW/kE7ILPbYNS1wLLhS7D9p28LbZeVfg6LBs1Czs3OsKl8oTGkIfqJME74SkREXk0mhBBSF+FuOTk5CAgIQHZ2Nvz9/aUup9T7dvxEXL8QC7tCDQDQ5p2ETPMXIGQQFj/YFDVhUQdBZrdAix14/INX2bGaiIhKzNPf3wxFdE82LlmIc+tzYdRFAbLCJxxldgsqRe7DE+PGSVAdERGVBQxFbsBQ5D5Jm9bg0PKdsJnqA8IKue4kNBUVqN+5DZp36iJ1eUREVIoxFLkBQxEREVHp4+nvb3a0JiIiIgJDEREREREAhiIiIiIiAAxFRERERAAYioiIiIgAMBQRERERAWAoIiIiIgLAUEREREQEwI2haPLkyWjVqhX0ej0Ci5j36uDBg3j66acRHh4OnU6H+vXrY+bMmYW227p1K5o3bw6NRoPatWtj4cKF7iqZiIiIyjG3hSKz2YzHH38cL774YpHrk5KSEBISgiVLluDIkSMYN24cxo4di08//dSxzZkzZ9C9e3d07NgRycnJeOWVV/D8889j3bp17iqbiIiIyim3T/OxcOFCvPLKK7h+/fpdtx02bBiOHTuGzZs3AwBGjx6N3377DSkpKY5tnnrqKVy/fh1r164tdg2c5oOIiKj0KdfTfGRnZ6NChQqO54mJiYiPj3faJiEhAYmJiZ4ujYiIiMo4pdQFFNi1axe+++47/Pbbb45lGRkZCA0NddouNDQUOTk5MBgM0Ol0Re7LZDLBZDI5nufk5LinaCIiIiozSnSmaMyYMZDJZHd8HD9+vMRFpKSkoGfPnhg/fjy6dOlS4tf/25QpUxAQEOB4hIeH3/c+iYiIqGwr0ZmiUaNGoX///nfcpmbNmiUq4OjRo+jcuTOGDBmCN99802ldWFgYMjMznZZlZmbC39//tmeJAGDs2LEYOXKk43l2djYiIiJ4xoiIiKgUKfjednP3Z4cShaLg4GAEBwe7rPEjR46gU6dO6NevHyZPnlxofVxcHFavXu20bMOGDYiLi7vjfjUaDTQajeP55cuXAYBnjIiIiEqhK1euICAgwO3tuK1PUVpaGq5evYq0tDTYbDYkJycDAGrXrg1fX1+kpKSgU6dOSEhIwMiRI5GRkQEAUCgUjuA1dOhQfPrpp3j99dcxcOBAbN68GcuXL3fqd1QcBZ2309LSPHJQy6qcnByEh4cjPT2dd/HdJx5L1+GxdA0eR9fhsXSdgis9t96E5U5uC0Vvv/02Fi1a5HjerFkzAMCWLVvQoUMH/PDDD7h06RKWLFmCJUuWOLaLjIzE2bNnAQA1atTAb7/9hhEjRmDmzJmoVq0avvrqKyQkJJSoFrk8v+tUQEAAP6Au4O/vz+PoIjyWrsNj6Ro8jq7DY+k6Bd/j7ub2cYq8Accpcg0eR9fhsXQdHkvX4HF0HR5L1ynX4xQRERERSaVchCKNRoPx48c7db6mkuNxdB0eS9fhsXQNHkfX4bF0HU8fy3Jx+YyIiIjobsrFmSIiIiKiu2EoIiIiIgJDEREREREAhiIiIiIiAOUgFH322WeoXr06tFotYmNjsWfPHqlL8ioTJkwoNKlvvXr1HOuNRiOGDRuGihUrwtfXF7179y40H11aWhq6d+8OvV6PkJAQvPbaa7BarZ5+Kx63fft29OjRA1WqVIFMJsPPP//stF4IgbfffhuVK1eGTqdDfHw8Tp486bTN1atX0adPH/j7+yMwMBCDBg3CzZs3nbY5dOgQ2rZtC61Wi/DwcHzwwQfufmsed7dj2b9//0Kf065duzptw2OZPxn2Aw88AD8/P4SEhKBXr15ITU112sZVv9Nbt25F8+bNodFoULt2bSxcuNDdb8+jinMsO3ToUOhzOXToUKdtyvux/Pzzz9G4cWPHQJZxcXFYs2aNY73XfR5FGbZs2TKhVqvF/PnzxZEjR8TgwYNFYGCgyMzMlLo0rzF+/HjRoEEDcfHiRcfj0qVLjvVDhw4V4eHhYtOmTWLfvn3iP//5j2jVqpVjvdVqFQ0bNhTx8fHiwIEDYvXq1aJSpUpi7NixUrwdj1q9erUYN26c+OmnnwQAsWLFCqf1U6dOFQEBAeLnn38WBw8eFP/9739FjRo1hMFgcGzTtWtX0aRJE/HHH3+IHTt2iNq1a4unn37asT47O1uEhoaKPn36iJSUFPHtt98KnU4n5s6d66m36RF3O5b9+vUTXbt2dfqcXr161WkbHkshEhISxIIFC0RKSopITk4WDz30kIiIiBA3b950bOOK3+k///xT6PV6MXLkSHH06FExa9YsoVAoxNq1az36ft2pOMeyffv2YvDgwU6fy+zsbMd6HkshfvnlF/Hbb7+JEydOiNTUVPHGG28IlUolUlJShBDe93ks06GoZcuWYtiwYY7nNptNVKlSRUyZMkXCqrzL+PHjRZMmTYpcd/36daFSqcT333/vWHbs2DEBQCQmJgoh8r/M5HK5yMjIcGzz+eefC39/f2Eymdxauzf59xe53W4XYWFhYtq0aY5l169fFxqNRnz77bdCCCGOHj0qAIi9e/c6tlmzZo2QyWTi/PnzQgghZs+eLYKCgpyO5ejRo0VUVJSb35F0bheKevbsedvX8FgWLSsrSwAQ27ZtE0K47nf69ddfFw0aNHBq68knnxQJCQnufkuS+fexFCI/FL388su3fQ2PZdGCgoLEV1995ZWfxzJ7+cxsNiMpKQnx8fGOZXK5HPHx8UhMTJSwMu9z8uRJVKlSBTVr1kSfPn2QlpYGAEhKSoLFYnE6hvXq1UNERITjGCYmJqJRo0YIDQ11bJOQkICcnBwcOXLEs2/Ei5w5cwYZGRlOxy4gIACxsbFOxy4wMBAtWrRwbBMfHw+5XI7du3c7tmnXrh3UarVjm4SEBKSmpuLatWseejfeYevWrQgJCUFUVBRefPFFXLlyxbGOx7Jo2dnZAP6ZFNtVv9OJiYlO+yjYpiz/3/rvY1lg6dKlqFSpEho2bIixY8ciLy/PsY7H0pnNZsOyZcuQm5uLuLg4r/w8um1CWKldvnwZNpvN6UACQGhoKI4fPy5RVd4nNjYWCxcuRFRUFC5evIiJEyeibdu2SElJQUZGBtRqNQIDA51eExoaioyMDABARkZGkce4YF15VfDeizo2tx67kJAQp/VKpRIVKlRw2qZGjRqF9lGwLigoyC31e5uuXbvi0UcfRY0aNXD69Gm88cYb6NatGxITE6FQKHgsi2C32/HKK6+gdevWaNiwIQC47Hf6dtvk5OTAYDBAp9O54y1JpqhjCQDPPPMMIiMjUaVKFRw6dAijR49GamoqfvrpJwA8lgUOHz6MuLg4GI1G+Pr6YsWKFYiOjkZycrLXfR7LbCii4unWrZvj340bN0ZsbCwiIyOxfPnyMvHLSGXDU0895fh3o0aN0LhxY9SqVQtbt25F586dJazMew0bNgwpKSnYuXOn1KWUerc7lkOGDHH8u1GjRqhcuTI6d+6M06dPo1atWp4u02tFRUUhOTkZ2dnZ+OGHH9CvXz9s27ZN6rKKVGYvn1WqVAkKhaJQL/bMzEyEhYVJVJX3CwwMRN26dXHq1CmEhYXBbDbj+vXrTtvcegzDwsKKPMYF68qrgvd+p89fWFgYsrKynNZbrVZcvXqVx/cuatasiUqVKuHUqVMAeCz/bfjw4Vi1ahW2bNmCatWqOZa76nf6dtv4+/uXuT+mbncsixIbGwsATp9LHktArVajdu3aiImJwZQpU9CkSRPMnDnTKz+PZTYUqdVqxMTEYNOmTY5ldrsdmzZtQlxcnISVebebN2/i9OnTqFy5MmJiYqBSqZyOYWpqKtLS0hzHMC4uDocPH3b6QtqwYQP8/f0RHR3t8fq9RY0aNRAWFuZ07HJycrB7926nY3f9+nUkJSU5ttm8eTPsdrvjP9e4uDhs374dFovFsc2GDRsQFRVV5i73lMRff/2FK1euoHLlygB4LAsIITB8+HCsWLECmzdvLnS50FW/03FxcU77KNimLP3ferdjWZTk5GQAcPpc8lgWZrfbYTKZvPPzWPJ+46XHsmXLhEajEQsXLhRHjx4VQ4YMEYGBgU692Mu7UaNGia1bt4ozZ86I33//XcTHx4tKlSqJrKwsIUT+7ZIRERFi8+bNYt++fSIuLk7ExcU5Xl9wu2SXLl1EcnKyWLt2rQgODi4Xt+TfuHFDHDhwQBw4cEAAENOnTxcHDhwQ586dE0Lk35IfGBgoVq5cKQ4dOiR69uxZ5C35zZo1E7t37xY7d+4UderUcbqN/Pr16yI0NFQ899xzIiUlRSxbtkzo9foydRu5EHc+ljdu3BCvvvqqSExMFGfOnBEbN24UzZs3F3Xq1BFGo9GxDx5LIV588UUREBAgtm7d6nSbeF5enmMbV/xOF9wC/dprr4ljx46Jzz77rEzdRi7E3Y/lqVOnxKRJk8S+ffvEmTNnxMqVK0XNmjVFu3btHPvgsRRizJgxYtu2beLMmTPi0KFDYsyYMUImk4n169cLIbzv81imQ5EQQsyaNUtEREQItVotWrZsKf744w+pS/IqTz75pKhcubJQq9WiatWq4sknnxSnTp1yrDcYDOKll14SQUFBQq/Xi0ceeURcvHjRaR9nz54V3bp1EzqdTlSqVEmMGjVKWCwWT78Vj9uyZYsAUOjRr18/IUT+bflvvfWWCA0NFRqNRnTu3FmkpqY67ePKlSvi6aefFr6+vsLf318MGDBA3Lhxw2mbgwcPijZt2giNRiOqVq0qpk6d6qm36DF3OpZ5eXmiS5cuIjg4WKhUKhEZGSkGDx5c6I8bHktR5DEEIBYsWODYxlW/01u2bBFNmzYVarVa1KxZ06mNsuBuxzItLU20a9dOVKhQQWg0GlG7dm3x2muvOY1TJASP5cCBA0VkZKRQq9UiODhYdO7c2RGIhPC+z6NMCCFKfn6JiIiIqGwps32KiIiIiEqCoYiIiIgIDEVEREREABiKiIiIiAAwFBEREREBYCgiIiIiAsBQRERERASAoYiIiIgIAEMREbnB1q1bIZPJCk306CmbNm1C/fr1YbPZ3NbGf/7zH/z4449u2z8ReR5HtCai+9KhQwc0bdoUH3/8sWOZ2WzG1atXERoaCplM5vGaYmJiMHLkSPTp08dtbaxatQojRoxAamoq5HL+fUlUFvA3mYhcTq1WIywsTJJAtHPnTpw+fRq9e/d2azvdunXDjRs3sGbNGre2Q0Sew1BERPesf//+2LZtG2bOnAmZTAaZTIazZ88Wuny2cOFCBAYGYtWqVYiKioJer8djjz2GvLw8LFq0CNWrV0dQUBD+97//OV3yMplMePXVV1G1alX4+PggNjYWW7duvWNNy5Ytw4MPPgitVutYNmHCBDRt2hTz589HREQEfH198dJLL8Fms+GDDz5AWFgYQkJCMHnyZMdrhBCYMGECIiIioNFoUKVKFfzvf/9zrFcoFHjooYewbNky1xxMIpKcUuoCiKj0mjlzJk6cOIGGDRti0qRJAIDg4GCcPXu20LZ5eXn45JNPsGzZMty4cQOPPvooHnnkEQQGBmL16tX4888/0bt3b7Ru3RpPPvkkAGD48OE4evQoli1bhipVqmDFihXo2rUrDh8+jDp16hRZ044dO/DMM88UWn769GmsWbMGa9euxenTp/HYY4/hzz//RN26dbFt2zbs2rULAwcORHx8PGJjY/Hjjz9ixowZWLZsGRo0aICMjAwcPHjQaZ8tW7bE1KlT7/MoEpG3YCgionsWEBAAtVoNvV6PsLCwO25rsVjw+eefo1atWgCAxx57DIsXL0ZmZiZ8fX0RHR2Njh07YsuWLXjyySeRlpaGBQsWIC0tDVWqVAEAvPrqq1i7di0WLFiA9957r8h2zp0759j+Vna7HfPnz4efn5+jrdTUVKxevRpyuRxRUVF4//33sWXLFsTGxiItLQ1hYWGIj4+HSqVCREQEWrZs6bTPKlWqID09HXa7nf2KiMoA/hYTkUfo9XpHIAKA0NBQVK9eHb6+vk7LsrKyAACHDx+GzWZD3bp14evr63hs27YNp0+fvm07BoPB6dJZgerVq8PPz8+prejoaKcwc2v7jz/+OAwGA2rWrInBgwdjxYoVsFqtTvvU6XSw2+0wmUwlPBpE5I14poiIPEKlUjk9l8lkRS6z2+0AgJs3b0KhUCApKQkKhcJpu1uD1L9VqlQJ165du+/2w8PDkZqaio0bN2LDhg146aWXMG3aNGzbts3xuqtXr8LHxwc6ne5Ob52ISgmGIiK6L2q12i3jATVr1gw2mw1ZWVlo27ZtiV539OhRl9Sg0+nQo0cP9OjRA8OGDUO9evVw+PBhNG/eHACQkpKCZs2auaQtIpIeQxER3Zfq1atj9+7dOHv2LHx9fVGhQgWX7Ldu3bro06cP+vbti48++gjNmjXDpUuXsGnTJjRu3Bjdu3cv8nUJCQlYtGjRfbe/cOFC2Gw2xMbGQq/XY8mSJdDpdIiMjHRss2PHDnTp0uW+2yIi78A+RUR0X1599VUoFApER0cjODgYaWlpLtv3ggUL0LdvX4waNQpRUVHo1asX9u7di4iIiNu+pk+fPjhy5AhSU1Pvq+3AwEB8+eWXaN26NRo3boyNGzfi119/RcWKFQEA58+fx65duzBgwID7aoeIvAdHtCaiMue1115DTk4O5s6d67Y2Ro8ejWvXruGLL75wWxtE5Fk8U0REZc64ceMQGRnp6DTtDiEhIXjnnXfctn8i8jyeKSIiIiICzxQRERERAWAoIiIiIgLAUEREREQEgKGIiIiICABDEREREREAhiIiIiIiAAxFRERERAAYioiIiIgAMBQRERERAQD+H9yLW2TdHstTAAAAAElFTkSuQmCC", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "_ = plot_traces(config_file='config.csv_wave.json', node_ids=range(5), report_name='membrane_potential')" + ] + }, + { + "cell_type": "markdown", + "id": "8ece4be3-20a9-4fc1-a77f-5e31fb04b9d4", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 3. Example: Voltage Clamps " + ] + }, + { + "cell_type": "markdown", + "id": "c5f4cad8-afb6-4446-b7c9-7ccbb12161a7", + "metadata": {}, + "source": [ + "Besides the current clamp, we can use a voltage clamp to set one or more cells to have a constant membrane voltage during some period of the simulation. And like the IClamp we can dynamically insert or remove a voltage clamp during any given simulation by simply editing our SONATA configuration json file. However, note that not all models allow us to insert a voltage clamp - in particular only models with conductance based mechanics allow voltage clamps (so for example we cannot clamp a integrate-and-fire neuron).\n", + "\n", + "In the network built using the file *build_network.voltage_clamp.py* we have a simple network of two cells, with cell #1 synapsing onto cell #2. As we might do with a simple in-vitro experiment we want to clamp only one cell and see the results it has on both cells. And we want to use a simple single-electrode clamp (eg `SEClamp`) which is the simplest want to fix the voltage of one or more cells. To do so we add the following to the SONATA configuration **\"inputs**\" section:\n", + "\n", + "```json\n", + " \"inputs\": {\n", + " \"se_clamp_1\": {\n", + " \"input_type\": \"clamp\",\n", + " \"module\": \"SEClamp\",\n", + " \"node_set\": {\n", + " \"population\": \"net\", \n", + " \"node_ids\": [0]\n", + " },\n", + " \"amp\": 20.0,\n", + " \"duration\": 1000.0,\n", + " \"delay\": 1000.0,\n", + " \"section_name\": \"soma\",\n", + " \"section_index\": 0,\n", + " \"section_dist\": 0.5\n", + " }\n", + " }\n", + "```\n", + "\n", + "* We must set **input_type** to `clamp` and **module** to 'SEClamp` to tell BMTK which type of stimuli that is being added.\n", + "* For the **node_set** we make sure to only attach a clamp to cell with `node_id=0`. (eg our source cell only, not the second cell in the network).\n", + "* We must specify the onset/**delay** of the clamp (in ms), the **duration** (ms) and the **amp** (mV) at which we want to hold our cell. In this case we will set the cell such that between times 1000 to 2000 ms it will be clamped to a membrane voltage of 20.0 mV.\n", + "* As with current-clamp we have options **section_name**, **section_index** and **section_dist** to determine where to place the clamp within the cell. If not specified BMTK will place it by default in the center of the soma.\n", + "* We can specifcy the **resistance** in nOhms - but default it will be set to 0.0.\n" + ] + }, + { + "cell_type": "markdown", + "id": "2057ec57-7361-4c50-89d2-4c7adacfe669", + "metadata": {}, + "source": [ + "
\n", + "⚠️ WARNING: Currently SEClamp can only handle simple single square-wave block. It does not support advance features like specified in the IClamp.\n", + "
" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "96eecab3-7360-41fc-972f-ab37947adaf4", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-06-24 17:25:45,034 [INFO] Created log file\n", + "2024-06-24 17:25:45,088 [INFO] Building cells.\n", + "2024-06-24 17:25:45,224 [INFO] Building recurrent connections\n", + "2024-06-24 17:25:45,235 [INFO] Running simulation for 3000.000 ms with the time step 0.100 ms\n", + "2024-06-24 17:25:45,236 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-06-24 17:25:45,237 [INFO] Block save every 5000 steps\n", + "2024-06-24 17:25:45,618 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-06-24 17:25:45,980 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-06-24 17:25:46,394 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-06-24 17:25:46,824 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-06-24 17:25:47,233 [INFO] step:25000 t_sim:2500.00 ms\n", + "2024-06-24 17:25:47,617 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-06-24 17:25:47,631 [INFO] Simulation completed in 2.396 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.voltage_clamp.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "edfb2be7-967a-436f-a02a-ce90b2a5ddde", + "metadata": {}, + "source": [ + "Now we can plot the membrane potential for the cell with the attached clamp (node-id 0) as-well-as the downstream cell in our network (node-id 1)" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "6c8174cd-c04a-42b5-98e5-920335826998", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "\n", + "_ = plot_traces(config_file='config.voltage_clamp.json', report_name='membrane_potential', population='net', node_ids=[0, 1])" + ] + }, + { + "cell_type": "markdown", + "id": "15e5748c-1900-4701-a0f9-17eb27ab7311", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 4. Example: Using sweep data from the Allen Cell-Types Database " + ] + }, + { + "cell_type": "markdown", + "id": "71d64192-60e2-4dc3-91cb-6ebf4a9cb3b8", + "metadata": {}, + "source": [ + "When creating the [Cell-Type Database at the Allen Insitute](https://celltypes.brain-map.org/data), a wide range of clamping stimuli was used in the process of optimizing the parameters for the thousands of human and mouse cell models. BMTK allows the option to download and import these stimuli directly into single and multi-cell simulations (for both BioNet and PointNet). Thus allowing users to apply the same conditions for their own models. Or as just a way to use much more advanced and realistic current-clamping stimuli that would be difficult to rebuild from scratch." + ] + }, + { + "cell_type": "markdown", + "id": "65fea49d-b3cf-44ef-9a67-a23afb061710", + "metadata": {}, + "source": [ + "### Step 1: Downloading the data\n", + "\n", + "First step is determine which experimental conditions we want to replicate and which stimlus types to use. Different cells were in the Allen Cell Types Database were optimized and tested against different types current clamps. For example if we look at the electrophysiology page for [cell 488683425](https://celltypes.brain-map.org/experiment/electrophysiology/488683425) we can see we have stimulus types including `Long square`, `Noise`, `Ramp`, `Short Square` and `Square`. And for each types there are different *sweeps* where amplitudes and times were varied between trials:\n", + "\n", + "
\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "If you click on the \"`Download Data`\" file it will download an ecephy.nwb file containing all the *sweep* available for said experiment. You just need to put it in a directory that can be accessed by BMTK. If you need a more programatically way to download multiple ecephy files you can use the AllenSDK, and BMTK also includes helper functions to download the data. You can run the following in a command-line:\n", + "```bash\n", + "$ python -m bmtk.utils.cell_types_db download_ephys_data --specimen-id=488683425 --download-dir=ecephys_inputs\n", + "```\n", + "\n", + "Or through the notebook" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "c8217ae3-8eee-481e-a28d-f2fcb9371cbb", + "metadata": {}, + "outputs": [], + "source": [ + "from bmtk.utils.cell_types_db import download_ephys_data\n", + "\n", + "download_ephys_data(\n", + " specimen_id=488683425,\n", + " download_dir='ephys_inputs'\n", + ")" + ] + }, + { + "cell_type": "markdown", + "id": "8eedfa8e-79e1-4623-af03-b947ab5a7c60", + "metadata": {}, + "source": [ + "Due to the size of the files and available internet speeds it may take some time to fully donwload the nwb file(s). But when it does it will save the file into the local *ephy_inputs/* directory." + ] + }, + { + "cell_type": "markdown", + "id": "01684297-31ef-4902-acc3-3d68a5bf6152", + "metadata": {}, + "source": [ + "### (Optional) Step 2: Downloading cell model data\n", + "\n", + "The downloaded ephys data can be used with any cell-type (even cells that are not from the Allen Cell-Types Database). However for testing and replication purposes you may want to run the downloaded ephys data on the original cell. If you click on `Select neuronal model` button on the page it will give you an option to download a possible GLIF Model (which can be ran using PointNet) or a Biophysical (ran using BioNet) model. This will download a zip file containing the *fit_parameters.json` which you can use as the **dynamics_params** in you SONATA network. Also for Biophysical models it will contain the file *reconstruction.swc* which is the **morphology** swc file for the specific cell. You can move these file(s) into their appropiate *components/* directory and rebuild/modify the SONATA network file so that **dynamics_params** and **morphology** points to the correct paths.\n", + "\n", + "Alternatively you can use BMTK to download, move and rename the files for you. In the command line you can run:\n", + "```bash\n", + "$ python -m bmtk.utils.cell_types_db download_model --specimen-id=488683425 --model-dir=\"components\" --model-type=\"Biophysical - all active\"\n", + "```\n", + "\n", + "or in python\n", + "```python\n", + "model_dir = download_model(\n", + " specimen_id=488683425,\n", + " model_dir='components',\n", + " model_type='Biophysical - all active'\n", + ")\n", + "```\n" + ] + }, + { + "cell_type": "markdown", + "id": "2bd6923b-ca11-4f27-b409-01333c08de24", + "metadata": {}, + "source": [ + "### Step 3: Set the configuration\n", + "\n", + "To use the downloaded ephys.nwb file we just need to create an \"inputs sections with **module** set to `iclamp` and **input_type** set to `allen`. Use the **file** option to point to the path of our downloaded nwb file. And finally we must select our **sweep** number. In this case we'll use swepp #35 which is just a 1 second long-square current.\n", + "\n", + "```json\n", + "\"inputs\": {\n", + " \"current_clamp_allen\": {\n", + " \"module\": \"IClamp\",\n", + " \"input_type\": \"allen\",\n", + " \"node_set\": \"all\",\n", + " \"file\": \"${INPUT_DIR}/488683423_ephys.nwb\",\n", + " \"sweep_id\": \"35\"\n", + " }\n", + "}\n", + "```\n" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "c4edd959-9003-41ac-b2b4-4e6c1c3418a6", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-05-05 14:54:45,142 [INFO] Created log file\n", + "2024-05-05 14:54:45,243 [INFO] Building cells.\n", + "2024-05-05 14:54:45,490 [INFO] Building recurrent connections\n", + "2024-05-05 14:54:45,857 [INFO] Running simulation for 3000.000 ms with the time step 0.100 ms\n", + "2024-05-05 14:54:45,858 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-05-05 14:54:45,859 [INFO] Block save every 5000 steps\n", + "2024-05-05 14:54:53,078 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-05-05 14:55:00,473 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-05-05 14:55:07,069 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-05-05 14:55:13,562 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-05-05 14:55:20,164 [INFO] step:25000 t_sim:2500.00 ms\n", + "2024-05-05 14:55:26,899 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-05-05 14:55:26,917 [INFO] Simulation completed in 41.06 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.cell_types.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "37cb859a-6e33-4f34-88e4-ff8c2e0b50c4", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "_ = plot_traces(config_file='config.cell_types.json', node_ids=[0], report_name='membrane_potential')" + ] + }, + { + "cell_type": "markdown", + "id": "7b037df1-25bf-4cc0-aa8d-cc6a9abd3628", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "## 5. Example: Extracellular Stimulation " + ] + }, + { + "cell_type": "markdown", + "id": "560a29e1-14eb-4e40-95ac-49a91725ed15", + "metadata": {}, + "source": [ + "
\n", + "⚠️ NOTE: This feature will only work for in BioNet using morphologically detailed cells.\n", + "
" + ] + }, + { + "cell_type": "markdown", + "id": "e2771c93-0e51-45d9-9f13-c44a23a52d3e", + "metadata": {}, + "source": [ + "The *BioNet* simulator has optional input stimulus an electrical field inpulse, thus allowing you to simulation of deep brain stimulation or transcranial magnetic stimulation. One more more electrodes (or a mesh) can be readily inserted and removed into most networks and used along side other stimuli through simple changes to the SONATA config. However, take note that this can only be applied to cells with a extracelluar condunctance, such as morphologically detailed \"biophysical\" cells or even simple single-compartment \"soma\" cells, but won't work for intergrate and fire type neurons in the network.\n", + "\n", + "When extracellular stimulation(s) are inserted into a simulation, the effect the generated filed has on the extracellular membrane of each cell compartment will depend on the geometric relationship between the cell and the electrode(s). Thus it is important that we specify the **x**, **y**, **z** attributes for each cell, plus make sure the cell is rotated properly (as specified by attributes **rotation_angle_x**, **rotation_angle_y**, **rotation_angle_z**). Once we have such a network (see *build_network.xstim.py*) we can go ahead and insert an extracellular stimulation into a simulation by adding to the **\"inputs\"** section of the SONATA config like such:\n", + "\n", + "``` json\n", + " \"inputs\": {\n", + " \"Extracellular_Stim\": {\n", + " \"module\": \"xstim\",\n", + " \"input_type\": \"lfp\",\n", + " \"node_set\": {\n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"positions_file\": \"components/stimulations/xstim_coords.csv\",\n", + " \"resistance\": 300.0,\n", + " \"waveform\": {\n", + " \"shape\": \"sin\",\n", + " \"del\": 1000.0,\n", + " \"amp\": 0.100,\n", + " \"dur\": 2000.0,\n", + " \"freq\": 8.0,\n", + " \"phase\": 0.0\n", + " }\n", + " }\n", + " }\n", + "```\n", + "* You must set **module** to \"xstim\" and **input_type** to \"lfp\" to signal to bmtk the mod of stimulus to apply\n", + "* The **node_set** is used to filter out which subset of cells in the network to apply the xstim. In this case we want to apply it to all \"biophysical\" type cells (in this example all are cells are \"biophysical\", but in mixed networks it's important otherwise bmtk can fail when applying stimlus to \"point-neuron\" type cells).\n", + "* Set the extracellular resistance between the electrode(s) and the cells to \n", + "* The **waveform** is set to generate a sin-wave shaped stimuli, starting at 1000 ms (**del**) that will run for 2000 ms (**dur**) with a maximum amplitude of .100 nA (**amp**). \n", + "* Lastly the **positions_file** is used to specify the coordinates and rotations of the electrodes. It should be a space-separated file with each row being the location of a different electrode. In our case we are only applying a single electrode\n" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "cb948bb9-33ac-4842-b3cd-ccaffb10f1f6", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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ippos_xpos_ypos_zrotation_xrotation_yrotation_z
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" + ], + "text/plain": [ + " ip pos_x pos_y pos_z rotation_x rotation_y rotation_z\n", + "0 0 6.18034 0.0 19.02113 0.0 0.0 0.0" + ] + }, + "execution_count": 1, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "pd.read_csv('components/stimulations/xstim_coords.csv', sep=' ')" + ] + }, + { + "cell_type": "markdown", + "id": "7772db17-16a0-4280-8d3e-c7af238e2747", + "metadata": {}, + "source": [ + "Now we can run the simulation as normal and show the varying results in terms of changes to cell membrane potentials" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "aeca0247-d98e-4f2b-bcd7-f61f1a992a90", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-06-24 14:27:32,061 [INFO] Created log file\n", + "2024-06-24 14:27:32,109 [INFO] Building cells.\n", + "2024-06-24 14:27:32,303 [INFO] Building recurrent connections\n", + "2024-06-24 14:27:32,480 [INFO] Running simulation for 4000.000 ms with the time step 0.100 ms\n", + "2024-06-24 14:27:32,481 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-06-24 14:27:32,482 [INFO] Block save every 10000 steps\n", + "2024-06-24 14:27:40,453 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-06-24 14:27:48,758 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-06-24 14:27:56,865 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-06-24 14:28:04,825 [INFO] step:40000 t_sim:4000.00 ms\n", + "2024-06-24 14:28:04,834 [INFO] Simulation completed in 32.35 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.xstim_sin.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "e8741b5f-dee6-4313-b8f7-ce7493693e8c", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.compartment import plot_traces\n", + "\n", + "_ = plot_traces(config_file='config.xstim_sin.json', report_name='membrane_potential', population='bio')" + ] + }, + { + "cell_type": "markdown", + "id": "845720d4-5c92-49b7-805e-7f4f8d5c9f84", + "metadata": {}, + "source": [ + "### More advanced wave-forms" + ] + }, + { + "cell_type": "markdown", + "id": "4274f41b-76fd-4890-99d8-0a85cb9e4737", + "metadata": {}, + "source": [ + "In the above example we set it so the stimulus will generate a sin-wave at the electrode site. BMTK has a number of built-in shapes available, including a sin-wave a a direct-current (**waveform**: \"dc\")\n", + "\n", + "```json\n", + " \"inputs\": {\n", + " \"Extracellular_Stim\": {\n", + " \"module\": \"xstim\",\n", + " \"input_type\": \"lfp\",\n", + " \"node_set\": {\n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"positions_file\": \"$STIM_DIR/xstim_coords.csv\",\n", + " \"resistance\": 300.0,\n", + " \"waveform\": {\n", + " \"shape\": \"dc\",\n", + " \"del\": 1000.0,\n", + " \"amp\": 0.400,\n", + " \"dur\": 1000.0\n", + " }\n", + " }\n", + " },\n", + "```" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "id": "fb774bc5-c74c-40e9-941a-029099f0e3ec", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-06-24 14:33:33,694 [INFO] Created log file\n", + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-06-24 14:33:33,718 [INFO] Building cells.\n", + "2024-06-24 14:33:33,993 [INFO] Building recurrent connections\n", + "2024-06-24 14:33:34,190 [INFO] Running simulation for 3000.000 ms with the time step 0.100 ms\n", + "2024-06-24 14:33:34,191 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-06-24 14:33:34,193 [INFO] Block save every 10000 steps\n", + "2024-06-24 14:33:49,082 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-06-24 14:34:03,980 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-06-24 14:34:19,004 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-06-24 14:34:19,019 [INFO] Simulation completed in 44.83 seconds \n" + ] + }, + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.xstim_dc.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()\n", + "\n", + "_ = plot_traces(config_file='config.xstim_dc.json', report_name='membrane_potential', population='bio')" + ] + }, + { + "cell_type": "markdown", + "id": "84dfa32f-bf00-403f-91df-63666e739c47", + "metadata": {}, + "source": [ + "Or alternatively, instead of having BMTK try to generate a shape for us, we can create the stimlus waveform ourselves. To do so we just need to create a space-separated csv file with columns **time** (ms) and **amplitude** (nA) and set the **waveform** attribute in our csv file to the path of the file.\n", + "\n", + "For example, the following lines of code will generate a slow ramping csv file that will go from -0.100 nA to 0.500 nA within a 3 second window, and save it under *./inputs/ramping_xstim.csv*" + ] + }, + { + "cell_type": "code", + "execution_count": 12, + "id": "ebeb4ca8-dbcd-4ca1-a351-db6b108d0ee1", + "metadata": {}, + "outputs": [ + { + "data": { + "text/plain": [ + "[]" + ] + }, + "execution_count": 12, + "metadata": {}, + "output_type": "execute_result" + }, + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "import numpy as np\n", + "import pandas as pd\n", + "import matplotlib.pyplot as plt\n", + "\n", + "time = np.linspace(0.0, 3000.0, 1000)\n", + "amplitude = time*0.0002-.100\n", + "\n", + "pd.DataFrame({\n", + " 'time': time,\n", + " 'amplitude': amplitude\n", + "}).to_csv('inputs/ramping_xstim.csv', sep=' ', index=False)\n", + "plt.plot(time, amplitude)" + ] + }, + { + "cell_type": "markdown", + "id": "8389a2d5-ab46-45ca-ba45-cb69d8384be9", + "metadata": {}, + "source": [ + "Then in our configuration file we just need to change the **waveform** attribute to point to the location of our customized file\n", + "\n", + "```json\n", + " \"inputs\": {\n", + " \"Extracellular_Stim\": {\n", + " \"module\": \"xstim\",\n", + " \"input_type\": \"lfp\",\n", + " \"node_set\": {\n", + " \"model_type\": \"biophysical\"\n", + " },\n", + " \"positions_file\": \"$STIM_DIR/xstim_coords.csv\",\n", + " \"resistance\": 300.0,\n", + " \"waveform\": \"inputs/ramping_xstim.csv\"\n", + " }\n", + " }\n", + "```\n", + "\n", + "And run it like before" + ] + }, + { + "cell_type": "code", + "execution_count": 15, + "id": "939f38fc-5376-4dd4-a22e-31666b6e753c", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-06-24 14:42:31,518 [INFO] Created log file\n", + "Mechanisms already loaded from path: ./components/mechanisms. Aborting.\n", + "2024-06-24 14:42:31,541 [INFO] Building cells.\n", + "2024-06-24 14:42:31,826 [INFO] Building recurrent connections\n", + "2024-06-24 14:42:32,029 [INFO] Running simulation for 3000.000 ms with the time step 0.100 ms\n", + "2024-06-24 14:42:32,030 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-06-24 14:42:32,031 [INFO] Block save every 10000 steps\n", + "2024-06-24 14:42:55,386 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-06-24 14:43:21,217 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-06-24 14:43:50,595 [INFO] step:30000 t_sim:3000.00 ms\n", + "2024-06-24 14:43:50,609 [INFO] Simulation completed in 78.58 seconds \n" + ] + }, + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.xstim_custom.json')\n", + "conf.build_env()\n", + "\n", + "graph = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=graph)\n", + "sim.run()\n", + "\n", + "_ = plot_traces(config_file='config.xstim_custom.json', report_name='membrane_potential', population='bio')" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "52c99a2e-c291-49ce-b924-6c896a6781b3", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3 (ipykernel)", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.9.7" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/04_opt_advanced_inputs/build_network.cell_types.py b/docs/tutorial/04_opt_advanced_inputs/build_network.cell_types.py new file mode 100644 index 00000000..fc58cd4e --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/build_network.cell_types.py @@ -0,0 +1,15 @@ +from bmtk.builder.networks import NetworkBuilder + +net = NetworkBuilder(f'bio_488683425_fullstub') +net.add_nodes( + N=1, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_allactive_fullaxon', + morphology=f'491766131_m.swc', + dynamics_params=f'491766131_fit.json', + specimen_id=488683425 +) + +net.build() +net.save(output_dir='network_cell_types') diff --git a/docs/tutorial/04_opt_advanced_inputs/build_network.current_clamp.py b/docs/tutorial/04_opt_advanced_inputs/build_network.current_clamp.py new file mode 100644 index 00000000..9e0e6ae9 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/build_network.current_clamp.py @@ -0,0 +1,20 @@ +import numpy as np +from bmtk.builder.networks import NetworkBuilder + +net = NetworkBuilder('net') +net.add_nodes( + N=5, + x=[0.0]*5, + y=np.linspace(0.0, -100.0, num=5), + z=[0.0]*5, + rotation_angle_xaxis=0.0, + rotation_angle_yaxis=0.0, + rotation_angle_zaxis=-3.646878266, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_perisomatic', + dynamics_params='472363762_fit.json', + morphology='Scnn1a_473845048_m.swc' +) +net.build() +net.save(output_dir='network_current_clamp') diff --git a/docs/tutorial/04_opt_advanced_inputs/build_network.voltage_clamp.py b/docs/tutorial/04_opt_advanced_inputs/build_network.voltage_clamp.py new file mode 100644 index 00000000..54763b7e --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/build_network.voltage_clamp.py @@ -0,0 +1,32 @@ +import numpy as np +from bmtk.builder.networks import NetworkBuilder + +net = NetworkBuilder('net') +net.add_nodes( + N=2, + x=[0.0]*2, + y=np.linspace(0.0, -100.0, num=2), + z=[0.0]*2, + rotation_angle_xaxis=0.0, + rotation_angle_yaxis=0.0, + rotation_angle_zaxis=-3.646878266, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_perisomatic', + dynamics_params='472363762_fit.json', + morphology='Scnn1a_473845048_m.swc' +) + +net.add_edges( + source={'node_id': 0}, target={'node_id': 1}, + connection_rule=20, + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + syn_weight=6.0e-01, + delay=30.0, + target_sections=['basal', 'apical', 'soma'], + distance_range=[0.0, 50.0] +) + +net.build() +net.save(output_dir='network_voltage_clamp') diff --git a/docs/tutorial/04_opt_advanced_inputs/build_network.xstim.py b/docs/tutorial/04_opt_advanced_inputs/build_network.xstim.py new file mode 100644 index 00000000..c52d4953 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/build_network.xstim.py @@ -0,0 +1,60 @@ +import os +import numpy as np + +from bmtk.builder import NetworkBuilder + + +cell_models = [ + { + 'model_name': 'Scnn1a', + 'ei': 'e', + 'morphology': 'Scnn1a_473845048_m.swc', + 'model_template': 'ctdb:Biophys1.hoc', + 'dynamics_params': '472363762_fit.json', + }, + { + 'model_name': 'Rorb', + 'ei': 'e', + 'morphology': 'Rorb_325404214_m.swc', + 'model_template': 'ctdb:Biophys1.hoc', + 'dynamics_params': '473863510_fit.json', + }, + { + 'model_name': 'Nr5a1', + 'ei': 'e', + 'morphology': 'Nr5a1_471087815_m.swc', + 'model_template': 'ctdb:Biophys1.hoc', + 'dynamics_params': '473863035_fit.json', + }, + { + 'model_name': 'PV1', + 'ei': 'i', + 'morphology': 'Pvalb_470522102_m.swc', + 'model_template': 'ctdb:Biophys1.hoc', + 'dynamics_params': '472912177_fit.json', + }, + { + 'model_name': 'PV2', + 'ei': 'i', + 'morphology': 'Pvalb_469628681_m.swc', + 'model_template': 'ctdb:Biophys1.hoc', + 'dynamics_params': '473862421_fit.json', + } +] + +bio_net = NetworkBuilder("bio") + +radius = 100.0 +dx = 2*np.pi/float(len(cell_models)) +for i, model_props in enumerate(cell_models): + bio_net.add_nodes( + model_type='biophysical', + model_processing='aibs_perisomatic', + x=[radius*np.cos(i*dx)], + y=[radius*np.sin(i*dx)], + z=[0.0], + **model_props + ) + +bio_net.build() +bio_net.save_nodes(output_dir='network_xstim') diff --git a/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/472363762_fit.json b/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/472363762_fit.json new file mode 100644 index 00000000..99f0eb30 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/472363762_fit.json @@ -0,0 +1,173 @@ +{ + "passive": [ + { + "ra": 138.28, + "cm": [ + { + "section": "soma", + "cm": 1.0 + }, + { + "section": "axon", + "cm": 1.0 + }, + { + "section": "apic", + "cm": 2.12 + }, + { + "section": "dend", + "cm": 2.12 + } + ], + "e_pas": -92.49911499023438 + } + ], + "axon_morph": [ + { 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00000000..e5f322e8 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/473863510_fit.json @@ -0,0 +1,173 @@ +{ + "passive": [ + { + "ra": 35.64, + "cm": [ + { + "section": "soma", + "cm": 1.0 + }, + { + "section": "axon", + "cm": 1.0 + }, + { + "section": "apic", + "cm": 2.19 + }, + { + "section": "dend", + "cm": 2.19 + } + ], + "e_pas": -85.07815551757812 + } + ], + "axon_morph": [ + { + "delete_axon": [ + "forsec axonal{delete_section()}", + " create axon[2]", + " axon[0]{", + " L= 30", + " diam = 1", + " nseg = 1+2*int(L/40)", + " all.append()", + " axonal.append()", + " }", + " axon[1]{", + " L= 30", + " diam = 1", + " nseg = 1+2*int(L/40)", + " all.append()", + " axonal.append()", + " }", + "", + "nSecAxonal = 2", + "connect axon(0), soma(0.5)", + "connect axon[1](0), axon[0](1) ", + "access soma" + ], + "setup_line": "create soma[1], dend[1], apic[1], axon[1]" + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 38 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -85.07815551757812 + } + ], + "genome": [ + { + "value": 0.00043788364247700001, + "section": "soma", + "name": "gbar_Im", + "mechanism": "Im" + }, + { + "value": 0.0019922075246600001, + "section": "soma", + "name": "gbar_Ih", + "mechanism": "Ih" + }, + { + "value": 0.71282189194800005, + "section": "soma", + "name": "gbar_NaTs", + "mechanism": "NaTs" + }, + { + "value": 0.0012493753876800001, + "section": "soma", + "name": "gbar_Nap", + "mechanism": "Nap" + }, + { + "value": 0.034836377263399998, + "section": "soma", + "name": "gbar_K_P", + "mechanism": "K_P" + }, + { + "value": 0.0166428509042, + "section": "soma", + "name": "gbar_K_T", + "mechanism": "K_T" + }, + { + "value": 0.00024972209054299998, + "section": "soma", + "name": "gbar_SK", + "mechanism": "SK" + }, + { + "value": 0.28059766435600003, + "section": "soma", + "name": "gbar_Kv3_1", + "mechanism": "Kv3_1" + }, + { + "value": 0.00015339031713199999, + "section": "soma", + "name": "gbar_Ca_HVA", + "mechanism": "Ca_HVA" + }, + { + "value": 0.0033469316039000004, + "section": "soma", + "name": "gbar_Ca_LVA", + "mechanism": "Ca_LVA" + }, + { + "value": 0.0040218816981199999, + "section": "soma", + "name": "gamma_CaDynamics", + "mechanism": "CaDynamics" + }, + { + "value": 991.140696832, + "section": "soma", + "name": "decay_CaDynamics", + "mechanism": "CaDynamics" + }, + { + "value": 0.00092865666454699993, + "section": "soma", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 0.00091423093354899986, + "section": "axon", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 3.8264043188599994e-06, + "section": "dend", + "name": "g_pas", + "mechanism": "" + }, + { + "value": 2.11145615996e-06, + "section": "apic", + "name": "g_pas", + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/491766131_fit.json b/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/491766131_fit.json new file mode 100644 index 00000000..11c6b471 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/biophysical_neuron_templates/491766131_fit.json @@ -0,0 +1,297 @@ +{ + "passive": [ + { + "ra": 100 + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 38 + ] + } + ], + "conditions": [ + { + "celsius": 34, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + }, + { + "ena": 53.0, + "section": "axon", + "ek": -107.0 + }, + { + "ena": 53.0, + "section": "apic", + "ek": -107.0 + }, + { + "ena": 53.0, + "section": "dend", + "ek": -107.0 + } + ], + "v_init": -90 + } + ], + "genome": [ + { + "section": "soma", + "name": "g_pas", + "value": "0.000119174", + "mechanism": "" + }, + { + "section": "soma", + "name": "e_pas", + "value": "-76.4024", + "mechanism": "" + }, + { + "section": "axon", + "name": "g_pas", + "value": "0.00147346", + "mechanism": "" + }, + { + "section": "axon", + "name": "e_pas", + "value": "-64.8595", + "mechanism": "" + }, + { + "section": "apic", + "name": "g_pas", + "value": "0.00041148", + "mechanism": "" + }, + { + "section": "apic", + "name": "e_pas", + "value": "-81.3599", + "mechanism": "" + }, + { + "section": "dend", + "name": "g_pas", + "value": "9.57001e-05", + "mechanism": "" + }, + { + "section": "dend", + "name": "e_pas", + "value": "-88.2554", + "mechanism": "" + }, + { + "section": "soma", + "name": "cm", + "value": "4.21567", + "mechanism": "" + }, + { + "section": "soma", + "name": "Ra", + "value": "133.577", + "mechanism": "" + }, + { + "section": "axon", + "name": "cm", + "value": "9.0228", + "mechanism": "" + }, + { + "section": "axon", + "name": "Ra", + "value": "80.3823", + "mechanism": "" + }, + { + "section": "apic", + "name": "cm", + "value": "8.34542", + "mechanism": "" + }, + { + "section": "apic", + "name": "Ra", + "value": "136.032", + "mechanism": "" + }, + { + "section": "dend", + "name": "cm", + "value": "2.11248", + "mechanism": "" + }, + { + "section": "dend", + "name": "Ra", + "value": "68.355", + "mechanism": "" + }, + { + "section": "axon", + "name": "gbar_NaV", + "value": "0.035766", + "mechanism": "NaV" + }, + { + "section": "axon", + "name": "gbar_K_T", + "value": "7.51307e-05", + "mechanism": "K_T" + }, + { + "section": "axon", + "name": "gbar_Kd", + "value": "0.00700751", + "mechanism": "Kd" + }, + { + "section": "axon", + "name": "gbar_Kv2like", + "value": "0.0675078", + "mechanism": "Kv2like" + }, + { + "section": "axon", + "name": "gbar_Kv3_1", + "value": "0.592911", + "mechanism": "Kv3_1" + }, + { + "section": "axon", + "name": "gbar_SK", + "value": "0.000701147", + "mechanism": "SK" + }, + { + "section": "axon", + "name": "gbar_Ca_HVA", + "value": "2.17253e-06", + "mechanism": "Ca_HVA" + }, + { + "section": "axon", + "name": "gbar_Ca_LVA", + "value": "0.00698184", + "mechanism": "Ca_LVA" + }, + { + "section": "axon", + "name": "gamma_CaDynamics", + "value": "0.0416279", + "mechanism": "CaDynamics" + }, + { + "section": "axon", + "name": "decay_CaDynamics", + "value": "226.076", + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "gbar_NaV", + "value": "0.0499779", + "mechanism": "NaV" + }, + { + "section": "soma", + "name": "gbar_SK", + "value": "0.000733676", + "mechanism": "SK" + }, + { + "section": "soma", + "name": "gbar_Kv3_1", + "value": "0.186718", + "mechanism": "Kv3_1" + }, + { + "section": "soma", + "name": "gbar_Ca_HVA", + "value": "9.96973e-05", + "mechanism": "Ca_HVA" + }, + { + "section": "soma", + "name": "gbar_Ca_LVA", + "value": "0.00344495", + "mechanism": "Ca_LVA" + }, + { + "section": "soma", + "name": "gamma_CaDynamics", + "value": "0.0177038", + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "decay_CaDynamics", + "value": "42.2507", + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "gbar_Ih", + "value": "1.07608e-07", + "mechanism": "Ih" + }, + { + "section": "apic", + "name": "gbar_NaV", + "value": "0.000375636", + "mechanism": "NaV" + }, + { + "section": "apic", + "name": "gbar_Kv3_1", + "value": "0.797015", + "mechanism": "Kv3_1" + }, + { + "section": "apic", + "name": "gbar_Im_v2", + "value": "0.00854163", + "mechanism": "Im_v2" + }, + { + "section": "apic", + "name": "gbar_Ih", + "value": "7.40408e-07", + "mechanism": "Ih" + }, + { + "section": "dend", + "name": "gbar_NaV", + "value": "0.0472215", + "mechanism": "NaV" + }, + { + "section": "dend", + "name": "gbar_Kv3_1", + "value": "0.186859", + "mechanism": "Kv3_1" + }, + { + "section": "dend", + "name": "gbar_Im_v2", + "value": "0.00132163", + "mechanism": "Im_v2" + }, + { + "section": "dend", + "name": "gbar_Ih", + "value": "9.18815e-06", + "mechanism": "Ih" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/CaDynamics.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/CaDynamics.mod new file mode 100644 index 00000000..12af0659 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/CaDynamics.mod @@ -0,0 +1,40 @@ +: Dynamics that track inside calcium concentration +: modified from Destexhe et al. 1994 + +NEURON { + SUFFIX CaDynamics + USEION ca READ ica WRITE cai + RANGE decay, gamma, minCai, depth +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + FARADAY = (faraday) (coulombs) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) +} + +PARAMETER { + gamma = 0.05 : percent of free calcium (not buffered) + decay = 80 (ms) : rate of removal of calcium + depth = 0.1 (um) : depth of shell + minCai = 1e-4 (mM) +} + +ASSIGNED {ica (mA/cm2)} + +INITIAL { + cai = minCai +} + +STATE { + cai (mM) +} + +BREAKPOINT { SOLVE states METHOD cnexp } + +DERIVATIVE states { + cai' = -(10000)*(ica*gamma/(2*FARADAY*depth)) - (cai - minCai)/decay +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ca_HVA.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ca_HVA.mod new file mode 100644 index 00000000..84db2d3c --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ca_HVA.mod @@ -0,0 +1,82 @@ +: Reference: Reuveni, Friedman, Amitai, and Gutnick, J.Neurosci. 1993 + +NEURON { + SUFFIX Ca_HVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + UNITSOFF + : if((v == -27) ){ + : v = v+0.0001 + : } + :mAlpha = (0.055*(-27-v))/(exp((-27-v)/3.8) - 1) + mAlpha = 0.055 * vtrap(-27 - v, 3.8) + mBeta = (0.94*exp((-75-v)/17)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + hAlpha = (0.000457*exp((-13-v)/50)) + hBeta = (0.0065/(exp((-v-15)/28)+1)) + hInf = hAlpha/(hAlpha + hBeta) + hTau = 1/(hAlpha + hBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ca_LVA.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ca_LVA.mod new file mode 100644 index 00000000..ab151d0e --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ca_LVA.mod @@ -0,0 +1,69 @@ +: Comment: LVA ca channel. Note: mtau is an approximation from the plots +: Reference: Avery and Johnston 1996, tau from Randall 1997 +: Comment: shifted by -10 mv to correct for junction potential +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Ca_LVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1.0000/(1+ exp((v - -30.000)/-6)) + mTau = (5.0000 + 20.0000/(1+exp((v - -25.000)/5)))/qt + hInf = 1.0000/(1+ exp((v - -80.000)/6.4)) + hTau = (20.0000 + 50.0000/(1+exp((v - -40.000)/7)))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ih.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ih.mod new file mode 100644 index 00000000..73b97d84 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Ih.mod @@ -0,0 +1,71 @@ +: Reference: Kole,Hallermann,and Stuart, J. Neurosci. 2006 + +NEURON { + SUFFIX Ih + NONSPECIFIC_CURRENT ihcn + RANGE gbar, g, ihcn +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + ehcn = -45.0 (mV) +} + +ASSIGNED { + v (mV) + ihcn (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ihcn = g*(v-ehcn) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + : if(v == -154.9){ + : v = v + 0.0001 + : } + :mAlpha = 0.001*6.43*(v+154.9)/(exp((v+154.9)/11.9)-1) + mAlpha = 0.001 * 6.43 * vtrap(v + 154.9, 11.9) + mBeta = 0.001*193*exp(v/33.1) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Im.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Im.mod new file mode 100644 index 00000000..d6112d57 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Im.mod @@ -0,0 +1,62 @@ +: Reference: Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Im + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Im_v2.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Im_v2.mod new file mode 100644 index 00000000..fc219f71 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Im_v2.mod @@ -0,0 +1,59 @@ +: Based on Im model of Vervaeke et al. (2006) + +NEURON { + SUFFIX Im_v2 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-30)/10) + mAlpha = 0.007 * exp( (6 * 0.4 * (v - (-48))) / 26.12 ) + mBeta = 0.007 * exp( (-6 * (1 - 0.4) * (v - (-48))) / 26.12 ) + + mInf = mAlpha / (mAlpha + mBeta) + mTau = (15 + 1 / (mAlpha + mBeta)) / qt +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/K_P.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/K_P.mod new file mode 100644 index 00000000..0a1238f9 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/K_P.mod @@ -0,0 +1,71 @@ +: Comment: The persistent component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + + +NEURON { + SUFFIX K_P + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + tauF = 1 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mInf = 1 / (1 + exp(-(v - (-14.3 + vshift)) / 14.6)) + if (v < -50 + vshift){ + mTau = tauF * (1.25+175.03*exp(-(v - vshift) * -0.026))/qt + } else { + mTau = tauF * (1.25+13*exp(-(v - vshift) * 0.026))/qt + } + hInf = 1/(1 + exp(-(v - (-54 + vshift))/-11)) + hTau = (360+(1010+24*(v - (-55 + vshift)))*exp(-((v - (-75 + vshift))/48)^2))/qt + UNITSON +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/K_T.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/K_T.mod new file mode 100644 index 00000000..c31beaff --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/K_T.mod @@ -0,0 +1,68 @@ +: Comment: The transient component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + +NEURON { + SUFFIX K_T + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + mTauF = 1.0 + hTauF = 1.0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1/(1 + exp(-(v - (-47 + vshift)) / 29)) + mTau = (0.34 + mTauF * 0.92*exp(-((v+71-vshift)/59)^2))/qt + hInf = 1/(1 + exp(-(v+66-vshift)/-10)) + hTau = (8 + hTauF * 49*exp(-((v+73-vshift)/23)^2))/qt + UNITSON +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kd.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kd.mod new file mode 100644 index 00000000..82cbe59a --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kd.mod @@ -0,0 +1,62 @@ +: Based on Kd model of Foust et al. (2011) + + +NEURON { + SUFFIX Kd + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * h + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h' = (hInf - h) / hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-23)/10) + mInf = 1 - 1 / (1 + exp((v - (-43)) / 8)) + mTau = 1 + hInf = 1 / (1 + exp((v - (-67)) / 7.3)) + hTau = 1500 +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kv2like.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kv2like.mod new file mode 100644 index 00000000..1cbdf843 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kv2like.mod @@ -0,0 +1,86 @@ +: Kv2-like channel +: Adapted from model implemented in Keren et al. 2005 +: Adjusted parameters to be similar to guangxitoxin-sensitive current in mouse CA1 pyramids from Liu and Bean 2014 + + +NEURON { + SUFFIX Kv2like + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mAlpha + mBeta + mTau + hInf + h1Tau + h2Tau +} + +STATE { + m + h1 + h2 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * m * (0.5 * h1 + 0.5 * h2) + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h1' = (hInf - h1) / h1Tau + h2' = (hInf - h2) / h2Tau +} + +INITIAL{ + rates() + m = mInf + h1 = hInf + h2 = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mAlpha = 0.12 * vtrap( -(v - 43), 11.0) + mBeta = 0.02 * exp(-(v + 1.27) / 120) + mInf = mAlpha / (mAlpha + mBeta) + mTau = 2.5 * (1 / (qt * (mAlpha + mBeta))) + + hInf = 1/(1 + exp((v + 58) / 11)) + h1Tau = (360 + (1010 + 23.7 * (v + 54)) * exp(-((v + 75) / 48)^2)) / qt + h2Tau = (2350 + 1380 * exp(-0.011 * v) - 210 * exp(-0.03 * v)) / qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kv3_1.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kv3_1.mod new file mode 100644 index 00000000..e2446577 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Kv3_1.mod @@ -0,0 +1,54 @@ +: Comment: Kv3-like potassium current + +NEURON { + SUFFIX Kv3_1 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + mInf = 1/(1+exp(((v -(18.700 + vshift))/(-9.700)))) + mTau = 0.2*20.000/(1+exp(((v -(-46.560 + vshift))/(-44.140)))) + UNITSON +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaTa.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaTa.mod new file mode 100644 index 00000000..fcf7bd39 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaTa.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTa + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -48 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -69 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaTs.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaTs.mod new file mode 100644 index 00000000..f753e718 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaTs.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTs + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -40 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -66 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaV.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaV.mod new file mode 100644 index 00000000..a7023956 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/NaV.mod @@ -0,0 +1,186 @@ +TITLE Mouse sodium current +: Kinetics of Carter et al. (2012) +: Based on 37 degC recordings from mouse hippocampal CA1 pyramids + +NEURON { + SUFFIX NaV + USEION na READ ena WRITE ina + RANGE g, gbar +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) +} + +PARAMETER { + gbar = .015 (S/cm2) + + : kinetic parameters + Con = 0.01 (/ms) : closed -> inactivated transitions + Coff = 40 (/ms) : inactivated -> closed transitions + Oon = 8 (/ms) : open -> Ineg transition + Ooff = 0.05 (/ms) : Ineg -> open transition + alpha = 400 (/ms) + beta = 12 (/ms) + gamma = 250 (/ms) : opening + delta = 60 (/ms) : closing + + alfac = 2.51 + btfac = 5.32 + + : Vdep + x1 = 24 (mV) : Vdep of activation (alpha) + x2 = -24 (mV) : Vdep of deactivation (beta) +} + +ASSIGNED { + + : rates + f01 (/ms) + f02 (/ms) + f03 (/ms) + f04 (/ms) + f0O (/ms) + f11 (/ms) + f12 (/ms) + f13 (/ms) + f14 (/ms) + f1n (/ms) + fi1 (/ms) + fi2 (/ms) + fi3 (/ms) + fi4 (/ms) + fi5 (/ms) + fin (/ms) + + b01 (/ms) + b02 (/ms) + b03 (/ms) + b04 (/ms) + b0O (/ms) + b11 (/ms) + b12 (/ms) + b13 (/ms) + b14 (/ms) + b1n (/ms) + bi1 (/ms) + bi2 (/ms) + bi3 (/ms) + bi4 (/ms) + bi5 (/ms) + bin (/ms) + + v (mV) + ena (mV) + ina (milliamp/cm2) + g (S/cm2) + celsius (degC) +} + +STATE { + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + C5 FROM 0 TO 1 + I1 FROM 0 TO 1 + I2 FROM 0 TO 1 + I3 FROM 0 TO 1 + I4 FROM 0 TO 1 + I5 FROM 0 TO 1 + O FROM 0 TO 1 + I6 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * O + ina = g * (v - ena) +} + +INITIAL { + rates(v) + SOLVE seqinitial +} + +KINETIC activation +{ + rates(v) + ~ C1 <-> C2 (f01,b01) + ~ C2 <-> C3 (f02,b02) + ~ C3 <-> C4 (f03,b03) + ~ C4 <-> C5 (f04,b04) + ~ C5 <-> O (f0O,b0O) + ~ O <-> I6 (fin,bin) + ~ I1 <-> I2 (f11,b11) + ~ I2 <-> I3 (f12,b12) + ~ I3 <-> I4 (f13,b13) + ~ I4 <-> I5 (f14,b14) + ~ I5 <-> I6 (f1n,b1n) + ~ C1 <-> I1 (fi1,bi1) + ~ C2 <-> I2 (fi2,bi2) + ~ C3 <-> I3 (fi3,bi3) + ~ C4 <-> I4 (fi4,bi4) + ~ C5 <-> I5 (fi5,bi5) + + CONSERVE C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +LINEAR seqinitial { : sets initial equilibrium + ~ I1*bi1 + C2*b01 - C1*( fi1+f01) = 0 + ~ C1*f01 + I2*bi2 + C3*b02 - C2*(b01+fi2+f02) = 0 + ~ C2*f02 + I3*bi3 + C4*b03 - C3*(b02+fi3+f03) = 0 + ~ C3*f03 + I4*bi4 + C5*b04 - C4*(b03+fi4+f04) = 0 + ~ C4*f04 + I5*bi5 + O*b0O - C5*(b04+fi5+f0O) = 0 + ~ C5*f0O + I6*bin - O*(b0O+fin) = 0 + + ~ C1*fi1 + I2*b11 - I1*( bi1+f11) = 0 + ~ I1*f11 + C2*fi2 + I3*b12 - I2*(b11+bi2+f12) = 0 + ~ I2*f12 + C3*fi3 + I4*bi3 - I3*(b12+bi3+f13) = 0 + ~ I3*f13 + C4*fi4 + I5*b14 - I4*(b13+bi4+f14) = 0 + ~ I4*f14 + C5*fi5 + I6*b1n - I5*(b14+bi5+f1n) = 0 + + ~ C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +PROCEDURE rates(v(mV) ) +{ + LOCAL qt + qt = 2.3^((celsius-37)/10) + + f01 = qt * 4 * alpha * exp(v/x1) + f02 = qt * 3 * alpha * exp(v/x1) + f03 = qt * 2 * alpha * exp(v/x1) + f04 = qt * 1 * alpha * exp(v/x1) + f0O = qt * gamma + f11 = qt * 4 * alpha * alfac * exp(v/x1) + f12 = qt * 3 * alpha * alfac * exp(v/x1) + f13 = qt * 2 * alpha * alfac * exp(v/x1) + f14 = qt * 1 * alpha * alfac * exp(v/x1) + f1n = qt * gamma + fi1 = qt * Con + fi2 = qt * Con * alfac + fi3 = qt * Con * alfac^2 + fi4 = qt * Con * alfac^3 + fi5 = qt * Con * alfac^4 + fin = qt * Oon + + b01 = qt * 1 * beta * exp(v/x2) + b02 = qt * 2 * beta * exp(v/x2) + b03 = qt * 3 * beta * exp(v/x2) + b04 = qt * 4 * beta * exp(v/x2) + b0O = qt * delta + b11 = qt * 1 * beta * exp(v/x2) / btfac + b12 = qt * 2 * beta * exp(v/x2) / btfac + b13 = qt * 3 * beta * exp(v/x2) / btfac + b14 = qt * 4 * beta * exp(v/x2) / btfac + b1n = qt * delta + bi1 = qt * Coff + bi2 = qt * Coff / (btfac) + bi3 = qt * Coff / (btfac^2) + bi4 = qt * Coff / (btfac^3) + bi5 = qt * Coff / (btfac^4) + bin = qt * Ooff +} + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Nap.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Nap.mod new file mode 100644 index 00000000..ef8021ec --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/Nap.mod @@ -0,0 +1,77 @@ +:Reference : Modeled according to kinetics derived from Magistretti & Alonso 1999 +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Nap + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + hInf + hTau + hAlpha + hBeta +} + +STATE { + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + rates() + g = gbar*mInf*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1.0/(1+exp((v- -52.6)/-4.6)) : assuming instantaneous activation as modeled by Magistretti and Alonso + + hInf = 1.0/(1+exp((v- -48.8)/10)) + hAlpha = 2.88e-6 * vtrap(v + 17, 4.63) + hBeta = 6.94e-6 * vtrap(-(v + 64.4), 2.63) + + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/SK.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/SK.mod new file mode 100644 index 00000000..8bfa3b72 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/SK.mod @@ -0,0 +1,56 @@ +: SK-type calcium-activated potassium current +: Reference : Kohler et al. 1996 + +NEURON { + SUFFIX SK + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gbar, g, ik +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (milli/liter) +} + +PARAMETER { + v (mV) + gbar = .000001 (mho/cm2) + zTau = 1 (ms) + ek (mV) + cai (mM) +} + +ASSIGNED { + zInf + ik (mA/cm2) + g (S/cm2) +} + +STATE { + z FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * z + ik = g * (v - ek) +} + +DERIVATIVE states { + rates(cai) + z' = (zInf - z) / zTau +} + +PROCEDURE rates(ca(mM)) { + if(ca < 1e-7){ + ca = ca + 1e-07 + } + zInf = 1/(1 + (0.00043 / ca)^4.8) +} + +INITIAL { + rates(cai) + z = zInf +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/exp1isyn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/exp1isyn.mod new file mode 100644 index 00000000..e8700401 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/exp1isyn.mod @@ -0,0 +1,52 @@ +COMMENT +Current-based version of the conductance-based model exp1syn (refer to the exp1syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + +NEURON { + POINT_PROCESS exp1isyn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*e +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/exp1syn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/exp1syn.mod new file mode 100644 index 00000000..6763e687 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/exp1syn.mod @@ -0,0 +1,42 @@ +NEURON { + POINT_PROCESS exp1syn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v-e) +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp1syn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp1syn.mod new file mode 100644 index 00000000..3490c587 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp1syn.mod @@ -0,0 +1,75 @@ +COMMENT +A model (# 1 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp1syn + RANGE e, i, tau_r, p0, tau_1 + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + g +} + +INITIAL { + n=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + + +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp2syn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp2syn.mod new file mode 100644 index 00000000..ce1d1625 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp2syn.mod @@ -0,0 +1,95 @@ +COMMENT +A model (# 2 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp2syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) + (uS) = (microsiemens) + +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_1 = baseline level of release probability + tau_1 = 5 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + tau_r + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p0 +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + tau_r' = (tau_r0-tau_r)/tau_FDR +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp3syn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp3syn.mod new file mode 100644 index 00000000..31869840 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp3syn.mod @@ -0,0 +1,102 @@ +COMMENT +A model (# 3 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp3syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D + a_D = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + tau_r + D + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 + D=1 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0*D + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p0*n*D + +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp4syn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp4syn.mod new file mode 100644 index 00000000..1ba964be --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp4syn.mod @@ -0,0 +1,83 @@ +COMMENT +A model (# 4 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by +solving Eqs (3,4) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + p = release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp4syn + RANGE e, i, tau_r, p0, tau_1,tau_f + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + p + g +} + +INITIAL { + n=1 + p=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + p' = 0 +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + p = p + p0*(1-p) + +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp5isyn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp5isyn.mod new file mode 100644 index 00000000..d6b6c105 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp5isyn.mod @@ -0,0 +1,113 @@ +COMMENT +Current-based version of the conductance-based model stp5syn (refer to the stp5syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp5isyn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of pb + pbtilde = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + p + tau_r + D + pb + g +} + +INITIAL { + n=1 + pb=pbtilde + p=pb + tau_r=tau_r0 + D=1 + g=0 + +} + +BREAKPOINT { + SOLVE state METHOD cnexp +: i = g*(v - e) + i = g*e + Pmax = n*p*D + +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp5syn.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp5syn.mod new file mode 100644 index 00000000..621fe9e1 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/stp5syn.mod @@ -0,0 +1,131 @@ +COMMENT +A model (# 5 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,4,5,8,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." +This file could be used to simulate simpler models by setting to zero the parameters in the unused equations(e.g, a_D,a_i,a_f). + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + pb = baseline vesicle release probability + p = vesicle release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where w is the static synaptic weight and Pmax is the activity-dependent factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1. + +Implemented by Sergey L. Gratiy, + +ENDCOMMENT + +: Declaring parameters as RANGE allows them to change as a function of a position alogn the cell +NEURON { + POINT_PROCESS stp5syn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +: Declaration of the default values of parameters +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of p0 + pbtilde = 0.5 + +} +: Declaration of dependent and external variables that collectively are called ASSIGNED +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +: Declaration of the state variables +STATE { + n + p + tau_r + D + pb + g +} + +: Initial conditions for the state variables +INITIAL { + n=1 + p=1 + tau_r=tau_r0 + D=1 + pb=pbtilde + g=0 + +} + +: Integration method + assignment statements +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p*D +} + +: Definition of teh dynamics between the presynaptic activations +DERIVATIVE state { + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + g' = -g/tau_1 + +} + +: This block defines what happens to the state variables at the moment presynaptic activation +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/vecevent.mod b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/vecevent.mod new file mode 100644 index 00000000..503dfd2e --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/mechanisms/modfiles/vecevent.mod @@ -0,0 +1,71 @@ +: Vector stream of events + +NEURON { + ARTIFICIAL_CELL VecStim +} + +ASSIGNED { + index + etime (ms) + space +} + +INITIAL { + index = 0 + element() + if (index > 0) { + net_send(etime - t, 1) + } +} + +NET_RECEIVE (w) { + if (flag == 1) { + net_event(t) + element() + if (index > 0) { + net_send(etime - t, 1) + } + } +} + +VERBATIM +extern double* vector_vec(); +extern int vector_capacity(); +extern void* vector_arg(); +ENDVERBATIM + +PROCEDURE element() { +VERBATIM + { void* vv; int i, size; double* px; + i = (int)index; + if (i >= 0) { + vv = *((void**)(&space)); + if (vv) { + size = vector_capacity(vv); + px = vector_vec(vv); + if (i < size) { + etime = px[i]; + index += 1.; + }else{ + index = -1.; + } + }else{ + index = -1.; + } + } + } +ENDVERBATIM +} + +PROCEDURE play() { +VERBATIM + void** vv; + vv = (void**)(&space); + *vv = (void*)0; + if (ifarg(1)) { + *vv = vector_arg(1); + } +ENDVERBATIM +} + + diff --git a/docs/tutorial/04_opt_advanced_inputs/components/morphologies/491766131_m.swc b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/491766131_m.swc new file mode 100644 index 00000000..f7c402fe --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/491766131_m.swc @@ -0,0 +1,4855 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): C:/Users/alexh/Desktop/Working/Rbp4-Cre_KL100;Ai14-203503.04.01.01_515570710_p_Dendrite.swc +# id,type,x,y,z,r,pid +1 1 357.4977 705.5311 27.0085 6.9553 -1 +2 3 351.3693 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4 373.4416 631.297 23.5488 0.17 1627 +1629 4 372.4441 631.5921 23.1963 0.1557 1628 +1630 4 371.3916 631.9605 22.813 0.1477 1629 +1631 4 370.4684 631.3278 22.4325 0.1571 1630 +1632 4 369.8666 630.3703 22.1458 0.1713 1631 +1633 4 368.7878 630.7021 21.9052 0.2135 1632 +1634 4 367.7674 631.21 21.7076 0.2142 1633 +1635 4 366.7447 631.7202 21.5678 0.2488 1634 +1636 4 365.7219 632.2327 21.4852 0.266 1635 +1637 4 364.6992 632.7464 21.4393 0.2977 1636 +1638 4 363.6787 633.2623 21.4141 0.2428 1637 +1639 4 362.6709 633.8057 21.4085 0.2347 1638 +1640 4 361.6664 634.3526 21.4152 0.2217 1639 +1641 4 360.6471 634.8502 21.427 0.1894 1640 +1642 4 359.5123 634.8994 21.4404 0.1686 1641 +1643 4 358.4335 634.5562 21.4715 0.1691 1642 +1644 4 357.4977 633.9144 21.5298 0.2095 1643 +1645 4 356.594 633.3653 21.5614 0.2069 1644 +1646 4 355.546 633.8035 21.5494 0.2349 1645 +1647 4 354.5645 634.0803 21.4659 0.2428 1646 +1648 4 353.4891 633.7382 21.3038 0.2414 1647 +1649 4 352.678 633.1491 21.0921 0.2023 1648 +1650 4 351.9688 632.3426 20.8516 0.1928 1649 +1651 4 350.8991 631.9891 20.6161 0.2123 1650 +1652 4 349.8066 631.6882 20.3675 0.2972 1651 +1653 4 348.7907 631.186 20.1228 0.3332 1652 +1654 4 347.8332 630.5991 19.8523 0.3021 1653 +1655 4 347.617 631.5097 19.5639 0.262 1654 +1656 4 346.6023 631.6882 19.3239 0.2175 1655 +1657 4 345.5029 631.3873 19.1349 0.2185 1656 +1658 4 344.6655 630.614 18.9871 0.2726 1657 +1659 4 343.9562 629.724 18.8451 0.3474 1658 +1660 4 342.9895 629.1256 18.6805 0.382 1659 +1661 4 342.0045 628.5479 18.517 0.3797 1660 +1662 4 341.1946 627.7586 18.3565 0.3538 1661 +1663 4 340.8491 626.6729 18.1686 0.297 1662 +1664 4 340.531 625.6399 17.9382 0.2344 1663 +1665 4 339.5243 625.1388 17.691 0.2534 1664 +1666 4 338.4158 624.9397 17.409 0.205 1665 +1667 4 337.5578 624.4844 17.1324 0.1782 1666 +1668 4 337.6802 623.377 16.9078 0.1665 1667 +1669 4 338.4124 622.7089 16.7177 0.1892 1668 +1670 4 339.4099 623.1128 16.5222 0.2445 1669 +1671 4 340.4247 623.2912 16.345 0.2827 1670 +1672 4 341.3044 622.6712 16.1804 0.3228 1671 +1673 4 341.4234 621.6073 15.9799 0.317 1672 +1674 4 340.7564 620.7664 15.8147 0.2386 1673 +1675 4 339.6376 620.6623 15.7181 0.1921 1674 +1676 4 338.5451 620.3294 15.6206 0.1992 1675 +1677 4 338.4947 619.3124 15.3566 0.2161 1676 +1678 4 339.5678 618.9829 14.6297 0.1144 1677 +1679 4 371.2349 630.8565 21.9428 0.1144 1631 +1680 4 372.2633 630.3875 21.5429 0.2655 1679 +1681 4 373.135 629.6919 21.4203 0.3344 1680 +1682 4 374.0022 629.0032 21.2836 0.3567 1681 +1683 4 374.6726 629.1554 21.037 0.3381 1682 +1684 4 374.6646 630.2776 20.7547 0.2512 1683 +1685 4 375.4894 630.6094 20.4842 0.2295 1684 +1686 4 376.0179 629.5947 20.2636 0.1994 1685 +1687 4 376.5716 628.6257 20.067 0.2086 1686 +1688 4 377.5749 628.1475 19.8464 0.2537 1687 +1689 4 378.5279 628.3454 19.6616 0.3145 1688 +1690 4 378.5874 629.4837 19.4919 0.3108 1689 +1691 4 378.37 630.5476 19.2844 0.2964 1690 +1692 4 378.0211 631.3507 19.0775 0.3069 1691 +1693 4 378.8745 632.1046 18.8706 0.2599 1692 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383.6244 645.9859 14.9498 0.3305 1715 +1717 4 384.7421 645.8692 14.6297 0.3432 1716 +1718 4 378.998 637.7022 30.6342 0.4451 1611 +1719 4 378.998 636.5605 30.7286 0.4987 1718 +1720 4 378.9855 635.4176 30.8204 0.4514 1719 +1721 4 378.9569 634.2736 30.8924 0.3878 1720 +1722 4 378.9271 633.1308 30.9467 0.3828 1721 +1723 4 378.8985 631.9868 30.9868 0.3969 1722 +1724 4 378.5164 631.6962 31.0136 0.1144 1723 +1725 4 377.6069 631.0052 31.2332 0.1896 1724 +1726 4 376.6986 630.3131 31.3275 0.2454 1725 +1727 4 375.7891 629.6221 31.4387 0.2837 1726 +1728 4 374.9117 628.89 31.5535 0.3278 1727 +1729 4 374.04 628.1498 31.6663 0.3119 1728 +1730 4 373.1682 627.4108 31.7733 0.2988 1729 +1731 4 372.2965 626.6718 31.8707 0.3082 1730 +1732 4 371.4248 625.9327 31.9595 0.2775 1731 +1733 4 370.4924 625.2704 32.0284 0.2253 1732 +1734 4 369.5498 624.6229 32.0807 0.221 1733 +1735 4 368.606 623.9754 32.1227 0.2198 1734 +1736 4 367.6622 623.329 32.1591 0.1859 1735 +1737 4 366.7378 622.6552 32.1997 0.1621 1736 +1738 4 365.8592 621.9253 32.2591 0.1571 1737 +1739 4 364.9875 621.1874 32.3341 0.1867 1738 +1740 4 364.1146 620.4495 32.4187 0.1669 1739 +1741 4 363.3367 619.6121 32.4988 0.15 1740 +1742 4 362.743 618.6374 32.559 0.1373 1741 +1743 4 362.1698 617.6479 32.5987 0.1373 1742 +1744 4 361.5955 616.6583 32.6214 0.1373 1743 +1745 4 361.0224 615.6676 32.632 0.1373 1744 +1746 4 360.5476 614.6277 32.6351 0.1373 1745 +1747 4 360.1243 613.565 32.6354 0.1373 1746 +1748 4 359.7056 612.501 32.6354 0.1373 1747 +1749 4 359.2869 611.436 32.6357 0.1373 1748 +1750 4 358.7458 610.4292 32.6357 0.1374 1749 +1751 4 357.969 609.5941 32.6357 0.1375 1750 +1752 4 357.1717 608.7739 32.636 0.1376 1751 +1753 4 356.3732 607.9536 32.636 0.1379 1752 +1754 4 355.5758 607.1334 32.6362 0.1384 1753 +1755 4 354.7773 606.3143 32.6365 0.1395 1754 +1756 4 353.9799 605.494 32.6371 0.1413 1755 +1757 4 353.0144 604.8877 32.6376 0.1446 1756 +1758 4 351.9951 604.3683 32.6385 0.1507 1757 +1759 4 350.906 604.0206 32.6399 0.1632 1758 +1760 4 349.8112 603.6877 32.6416 0.1803 1759 +1761 4 348.7175 603.3548 32.6441 0.2424 1760 +1762 4 347.6387 602.9738 32.6474 0.2087 1761 +1763 4 346.5691 602.5677 32.6522 0.1825 1762 +1764 4 345.5006 602.1593 32.6589 0.1868 1763 +1765 4 344.4321 601.7497 32.6682 0.167 1764 +1766 4 343.3087 601.5415 32.6813 0.1502 1765 +1767 4 342.1727 601.4111 32.6998 0.1376 1766 +1768 4 341.0298 601.4168 32.7261 0.1379 1767 +1769 4 339.9121 601.6593 32.7614 0.1383 1768 +1770 4 338.7979 601.9145 32.8079 0.1392 1769 +1771 4 337.7042 602.2485 32.881 0.1409 1770 +1772 4 336.6437 602.6649 32.9916 0.1441 1771 +1773 4 335.5844 603.0859 33.1282 0.1497 1772 +1774 4 334.525 603.5069 33.2777 0.1614 1773 +1775 4 333.4657 603.9268 33.4281 0.1769 1774 +1776 4 332.3469 604.1578 33.5423 0.2361 1775 +1777 4 331.2155 604.3306 33.6109 0.1967 1776 +1778 4 330.084 604.4999 33.6395 0.1627 1777 +1779 4 328.9538 604.6704 33.6378 0.1373 1778 +1780 4 327.8143 604.763 33.6014 0.1373 1779 +1781 4 326.6761 604.7321 33.5216 0.1373 1780 +1782 4 325.5378 604.8099 33.4281 0.1373 1781 +1783 4 324.4613 605.1932 33.3598 0.1373 1782 +1784 4 323.3859 605.5833 33.3178 0.1373 1783 +1785 4 322.3117 605.9768 33.3029 0.1373 1784 +1786 4 321.2009 606.2525 33.3158 0.1373 1785 +1787 4 320.0901 606.5259 33.3547 0.1373 1786 +1788 4 318.9792 606.7982 33.4121 0.1373 1787 +1789 4 317.8615 607.0396 33.49 0.1373 1788 +1790 4 316.7324 607.083 33.6356 0.1373 1789 +1791 4 315.6021 607.1208 33.8288 0.1373 1790 +1792 4 314.4639 607.1974 34.0158 0.1373 1791 +1793 4 313.3256 607.3118 34.1603 0.1373 1792 +1794 4 312.1919 607.4594 34.265 0.1373 1793 +1795 4 311.0593 607.6219 34.335 0.1373 1794 +1796 4 309.9268 607.7832 34.3767 0.1373 1795 +1797 4 308.7942 607.9445 34.4047 0.1373 1796 +1798 4 307.6822 608.2133 34.4378 0.1373 1797 +1799 4 306.6595 608.7235 34.4901 0.1373 1798 +1800 4 305.6379 609.2361 34.5562 0.1373 1799 +1801 4 304.6163 609.7486 34.6304 0.1373 1800 +1802 4 303.5947 610.2611 34.7074 0.1373 1801 +1803 4 302.5514 610.729 34.7777 0.1373 1802 +1804 4 301.4646 611.0848 34.827 0.1373 1803 +1805 4 300.3766 611.4394 34.8583 0.1373 1804 +1806 4 299.2887 611.794 34.8757 0.1373 1805 +1807 4 298.179 612.072 34.8838 0.1373 1806 +1808 4 297.035 612.056 34.886 0.1373 1807 +1809 4 295.891 612.04 34.886 0.1373 1808 +1810 4 294.747 612.0228 34.886 0.1373 1809 +1811 4 293.603 612.0068 34.886 0.1373 1810 +1812 4 292.4602 611.9908 34.886 0.1373 1811 +1813 4 291.3162 611.9748 34.886 0.1373 1812 +1814 4 290.1722 611.9588 34.886 0.1372 1813 +1815 4 289.0282 611.9428 34.886 0.1372 1814 +1816 4 287.8842 611.9256 34.886 0.1371 1815 +1817 4 286.7402 611.9096 34.886 0.1368 1816 +1818 4 285.5962 611.8936 34.886 0.1364 1817 +1819 4 284.4522 611.8776 34.886 0.1356 1818 +1820 4 283.3082 611.8673 34.886 0.1342 1819 +1821 4 282.1642 611.889 34.886 0.1313 1820 +1822 4 281.0213 611.9107 34.886 0.1271 1821 +1823 4 279.8773 611.9325 34.886 0.1144 1822 +1824 4 278.7333 611.9542 34.886 0.1144 1823 +1825 4 379.5517 631.0521 31.0192 0.4242 1723 +1826 4 380.2073 630.1152 31.052 0.3541 1825 +1827 4 380.8628 629.1783 31.0926 0.3533 1826 +1828 4 381.5183 628.2402 31.145 0.5249 1827 +1829 4 381.7791 627.1316 31.2351 0.5751 1828 +1830 4 381.8912 625.9968 31.3617 0.5964 1829 +1831 4 382.0022 624.8631 31.5112 0.5267 1830 +1832 4 382.1143 623.7294 31.6716 0.469 1831 +1833 4 382.2779 622.6003 31.8242 0.3591 1832 +1834 4 382.6451 621.5169 31.934 0.2845 1833 +1835 4 383.0112 620.4324 32.004 0.3187 1834 +1836 4 383.3773 619.349 32.0426 0.3464 1835 +1837 4 383.7491 618.2668 32.0586 0.3419 1836 +1838 4 384.122 617.1857 32.0608 0.2615 1837 +1839 4 384.495 616.1035 32.0561 0.2341 1838 +1840 4 384.8679 615.0224 32.0496 0.2786 1839 +1841 4 385.1848 613.875 32.0275 0.3559 1840 +1842 4 385.4902 612.7733 32.0093 0.4093 1841 +1843 4 385.8026 611.6728 31.9841 0.3736 1842 +1844 4 386.3105 610.6478 31.9508 0.2752 1843 +1845 4 386.8013 609.6147 31.904 0.2485 1844 +1846 4 387.2394 608.56 31.8259 0.2445 1845 +1847 4 387.6787 607.5063 31.724 0.2444 1846 +1848 4 388.1169 606.4516 31.6053 0.2091 1847 +1849 4 387.9567 606.161 31.5101 0.1144 1848 +1850 4 387.403 605.1588 31.3446 0.2195 1849 +1851 4 386.8733 604.1453 31.2844 0.238 1850 +1852 4 386.1835 603.2381 31.1898 0.2325 1851 +1853 4 385.2363 602.6203 31.0346 0.1859 1852 +1854 4 384.7375 601.6136 30.8605 0.1621 1853 +1855 4 384.5362 600.4879 30.6902 0.1571 1854 +1856 4 384.1483 599.4217 30.476 0.1867 1855 +1857 4 383.5878 598.4619 30.1896 0.1669 1856 +1858 4 383.0284 597.5032 29.8628 0.15 1857 +1859 4 382.6302 597.0788 29.6187 0.1373 1858 +1860 4 381.8455 596.2459 29.4434 0.1373 1859 +1861 4 381.1122 595.3811 29.3278 0.1373 1860 +1862 4 380.6877 594.3194 29.2659 0.1373 1861 +1863 4 380.3663 593.2212 29.2421 0.1373 1862 +1864 4 380.046 592.123 29.2354 0.1373 1863 +1865 4 379.7211 591.0259 29.2258 0.1373 1864 +1866 4 379.3607 589.9414 29.2124 0.1373 1865 +1867 4 378.9351 588.8797 29.1934 0.1374 1866 +1868 4 378.5713 587.7952 29.1668 0.1374 1867 +1869 4 378.2945 586.6855 29.1315 0.1376 1868 +1870 4 377.9925 585.5839 29.0825 0.1379 1869 +1871 4 377.5555 584.5291 29.0044 0.1383 1870 +1872 4 377.0601 583.5006 28.896 0.1391 1871 +1873 4 376.5648 582.4722 28.7666 0.1407 1872 +1874 4 376.0751 581.4391 28.6286 0.1435 1873 +1875 4 375.5878 580.4061 28.4892 0.1494 1874 +1876 4 375.0993 579.3719 28.3522 0.1571 1875 +1877 4 374.6142 578.3378 28.2204 0.1867 1876 +1878 4 374.1452 577.2956 28.0927 0.1669 1877 +1879 4 373.7139 576.2374 27.9675 0.15 1878 +1880 4 373.2838 575.1792 27.8432 0.1373 1879 +1881 4 372.8525 574.1198 27.7197 0.1373 1880 +1882 4 372.4017 573.0708 27.5965 0.1373 1881 +1883 4 371.8583 572.0652 27.4742 0.1373 1882 +1884 4 371.2715 571.0848 27.3526 0.1374 1883 +1885 4 370.6846 570.1044 27.2317 0.1374 1884 +1886 4 370.0977 569.1228 27.111 0.1376 1885 +1887 4 369.512 568.1413 26.9903 0.1379 1886 +1888 4 368.932 567.1563 26.8699 0.1383 1887 +1889 4 368.352 566.1713 26.7484 0.1392 1888 +1890 4 367.772 565.1863 26.6244 0.1409 1889 +1891 4 367.192 564.2014 26.4972 0.144 1890 +1892 4 366.5937 563.229 26.3626 0.1497 1891 +1893 4 365.8958 562.3286 26.2052 0.1608 1892 +1894 4 365.0893 561.5576 26.0285 0.1788 1893 +1895 4 364.0059 561.1903 25.8661 0.2248 1894 +1896 4 363.0381 560.6961 25.7082 0.2484 1895 +1897 4 362.3803 559.7695 25.5321 0.249 1896 +1898 4 361.6276 558.9138 25.3613 0.2294 1897 +1899 4 360.8039 558.121 25.2118 0.1803 1898 +1900 4 359.9813 557.3282 25.0796 0.1511 1899 +1901 4 359.1577 556.5354 24.9609 0.1393 1900 +1902 4 358.3134 555.7666 24.8592 0.1411 1901 +1903 4 357.4085 555.0665 24.7811 0.1444 1902 +1904 4 356.4601 554.4304 24.7201 0.1503 1903 +1905 4 355.4385 553.9191 24.6674 0.1619 1904 +1906 4 354.3689 553.5141 24.6156 0.1809 1905 +1907 4 353.2718 553.2041 24.5473 0.2281 1906 +1908 4 352.1507 552.9936 24.4507 0.2574 1907 +1909 4 351.2057 552.544 24.3407 0.2515 1908 +1910 4 350.6017 551.5807 24.2432 0.3033 1909 +1911 4 349.6979 550.9561 24.1604 0.3241 1910 +1912 4 348.6065 550.6152 24.0887 0.2698 1911 +1913 4 347.5827 550.1324 24.0153 0.2767 1912 +1914 4 346.6354 549.4929 23.9268 0.225 1913 +1915 4 345.6882 548.8546 23.8221 0.2138 1914 +1916 4 344.7398 548.2162 23.7006 0.2401 1915 +1917 4 343.7651 547.6214 23.5749 0.2887 1916 +1918 4 342.7699 547.0597 23.4441 0.2647 1917 +1919 4 341.7894 546.4774 23.294 0.2034 1918 +1920 4 340.7884 545.9317 23.1269 0.1705 1919 +1921 4 339.7028 545.7097 22.9594 0.1755 1920 +1922 4 338.7407 545.3173 22.7772 0.2063 1921 +1923 4 337.8461 544.6355 22.5624 0.2744 1922 +1924 4 336.7936 544.2283 22.335 0.347 1923 +1925 4 335.6725 544.0406 22.1119 0.399 1924 +1926 4 334.5662 544.2077 21.9131 0.3243 1925 +1927 4 333.81 544.9478 21.7126 0.3302 1926 +1928 4 333.3925 546.0037 21.5054 0.3268 1927 +1929 4 333.0516 547.094 21.3212 0.2749 1928 +1930 4 332.6683 548.1705 21.1436 0.286 1929 +1931 4 332.0037 549.0617 20.932 0.2436 1930 +1932 4 330.9455 549.2699 20.7264 0.2417 1931 +1933 4 329.8404 549.5204 20.5201 0.3231 1932 +1934 4 328.8348 550.0238 20.2756 0.2911 1933 +1935 4 327.7274 550.1622 20.008 0.3184 1934 +1936 4 326.9724 550.9332 19.7386 0.2922 1935 +1937 4 326.1452 551.7134 19.4995 0.273 1936 +1938 4 325.0848 552.1264 19.3242 0.3239 1937 +1939 4 324.0334 552.576 19.206 0.341 1938 +1940 4 322.9604 552.973 19.1349 0.4049 1939 +1941 4 321.8507 553.2498 19.0949 0.3672 1940 +1942 4 320.9709 553.9694 19.0742 0.3689 1941 +1943 4 320.1415 554.7565 19.0498 0.368 1942 +1944 4 319.136 555.2884 18.9916 0.2715 1943 +1945 4 318.1098 555.7941 18.9375 0.2095 1944 +1946 4 317.063 556.2414 18.9431 0.2132 1945 +1947 4 316.0048 556.6681 18.9907 0.2161 1946 +1948 4 314.8757 556.8512 19.0347 0.2415 1947 +1949 4 313.7466 557.0342 19.075 0.3051 1948 +1950 4 312.6174 557.2172 19.131 0.4576 1949 +1951 4 383.0764 597.0158 29.0072 0.1144 1858 +1952 4 383.5889 596.1441 28.3777 0.1381 1951 +1953 4 384.0522 595.3147 28.1249 0.1388 1952 +1954 4 383.7033 594.2737 27.7782 0.14 1953 +1955 4 383.9767 593.5118 27.3722 0.1423 1954 +1956 4 385.0098 593.0427 26.9564 0.1467 1955 +1957 4 386.005 592.4959 26.5457 0.1546 1956 +1958 4 386.6411 591.6196 26.0966 0.1697 1957 +1959 4 386.7635 590.5511 25.5861 0.1956 1958 +1960 4 386.9443 589.5318 24.9866 0.2534 1959 +1961 4 387.4751 588.548 24.4625 0.3145 1960 +1962 4 387.1376 587.5401 23.9952 0.3072 1961 +1963 4 386.6354 586.5231 23.5631 0.3075 1962 +1964 4 387.6204 586.117 23.1333 0.2406 1963 +1965 4 388.3605 585.2567 22.7461 0.2152 1964 +1966 4 389.2151 584.5108 22.3826 0.2613 1965 +1967 4 390.1715 583.9068 22.0192 0.238 1966 +1968 4 390.5227 582.8302 21.6642 0.265 1967 +1969 4 391.224 581.9425 21.3209 0.2028 1968 +1970 4 392.1083 581.2538 20.9975 0.1748 1969 +1971 4 392.3577 580.1418 20.7281 0.1571 1970 +1972 4 392.2513 579.0081 20.466 0.1868 1971 +1973 4 392.1895 577.887 20.2065 0.1671 1972 +1974 4 392.8016 576.9787 19.9178 0.1503 1973 +1975 4 393.8266 576.616 19.6658 0.1379 1974 +1976 4 394.0851 575.535 19.3998 0.1383 1975 +1977 4 393.9799 574.3978 19.1618 0.1391 1976 +1978 4 393.8117 573.2721 18.9417 0.1407 1977 +1979 4 393.6813 572.1487 18.7216 0.1436 1978 +1980 4 393.9467 571.142 18.548 0.1494 1979 +1981 4 393.9215 570.4236 18.4019 0.1571 1980 +1982 4 393.7362 569.4992 18.2339 0.1868 1981 +1983 4 394.4638 568.6732 18.0482 0.1671 1982 +1984 4 395.3904 568.0029 17.8783 0.1503 1983 +1985 4 396.4143 567.519 17.6893 0.1379 1984 +1986 4 397.4782 567.1151 17.4726 0.1383 1985 +1987 4 398.4277 566.5019 17.2483 0.1392 1986 +1988 4 398.7092 565.4517 16.9929 0.1409 1987 +1989 4 399.6084 564.874 16.758 0.1439 1988 +1990 4 400.0213 563.8856 16.4858 0.1502 1989 +1991 4 400.0076 562.7519 16.2201 0.1586 1990 +1992 4 399.6598 561.6823 15.9298 0.1894 1991 +1993 4 399.1816 560.6481 15.6545 0.1721 1992 +1994 4 398.6943 559.6173 15.3992 0.1594 1993 +1995 4 398.231 558.5786 15.1455 0.1551 1994 +1996 4 398.0125 557.4701 14.8932 0.1683 1995 +1997 4 398.39 556.397 14.6983 0.2048 1996 +1998 4 398.3511 555.261 14.5228 0.2132 1997 +1999 4 398.104 554.1513 14.35 0.1738 1998 +2000 4 398.08 553.0085 14.2257 0.1398 1999 +2001 4 398.3305 551.8919 14.1459 0.1144 2000 +2002 4 398.6085 550.7822 14.0669 0.1144 2001 +2003 4 388.8467 605.5764 31.5028 0.1992 1848 +2004 4 389.5778 604.6967 31.4191 0.2544 2003 +2005 4 389.9358 603.6202 31.3113 0.2285 2004 +2006 4 390.1818 602.5151 31.1864 0.2319 2005 +2007 4 390.4278 601.41 31.0607 0.2164 2006 +2008 4 391.1794 601.0919 30.3848 0.1144 2007 +2009 4 392.2536 600.7121 30.1896 0.1373 2008 +2010 4 393.3221 600.8025 30.1185 0.1373 2009 +2011 4 394.3482 601.0187 30.0023 0.1373 2010 +2012 4 395.4385 600.7281 29.822 0.1373 2011 +2013 4 396.5401 600.4581 29.6187 0.1373 2012 +2014 4 397.6601 600.2271 29.4406 0.1373 2013 +2015 4 398.7915 600.0703 29.2992 0.1373 2014 +2016 4 399.9332 600.0269 29.1894 0.1373 2015 +2017 4 401.0624 599.9067 29.0984 0.1373 2016 +2018 4 402.1675 599.6127 29.0074 0.1374 2017 +2019 4 403.2646 599.2958 28.9047 0.1374 2018 +2020 4 404.3628 598.979 28.7854 0.1375 2019 +2021 4 405.4553 598.6449 28.6496 0.1377 2020 +2022 4 406.5261 598.2514 28.4959 0.1382 2021 +2023 4 407.5786 597.8098 28.3262 0.139 2022 +2024 4 408.6299 597.3671 28.1476 0.1405 2023 +2025 4 409.6813 596.9255 27.9644 0.1432 2024 +2026 4 410.7326 596.4839 27.7799 0.1483 2025 +2027 4 411.7965 596.0698 27.608 0.1581 2026 +2028 4 412.8742 595.6831 27.4604 0.1738 2027 +2029 4 413.9438 595.2804 27.326 0.2151 2028 +2030 4 414.9391 594.7404 27.1802 0.2321 2029 +2031 4 415.7262 593.9362 26.9903 0.2099 2030 +2032 4 416.3885 593.0164 26.7498 0.2009 2031 +2033 4 417.1402 592.1824 26.465 0.2569 2032 +2034 4 418.0599 591.5452 26.1405 0.236 2033 +2035 4 419.093 591.0991 25.8079 0.2317 2034 +2036 4 420.2049 590.8829 25.5203 0.2855 2035 +2037 4 421.3455 590.7936 25.2949 0.3135 2036 +2038 4 422.1657 590.2148 25.1138 0.2564 2037 +2039 4 422.8167 589.2824 24.9466 0.2217 2038 +2040 4 423.7891 588.8134 24.7453 0.2695 2039 +2041 4 424.7764 588.2951 24.5171 0.2716 2040 +2042 4 425.8243 587.9291 24.2455 0.2382 2041 +2043 4 426.9488 587.8638 23.9072 0.2424 2042 +2044 4 428.0642 587.746 23.5136 0.2722 2043 +2045 4 429.1338 587.4417 23.0759 0.221 2044 +2046 4 430.0616 586.8789 22.5921 0.185 2045 +2047 4 431.0798 586.7576 22.0962 0.1758 2046 +2048 4 431.6678 587.6728 21.7202 0.2226 2047 +2049 4 432.6448 587.0779 21.4444 0.228 2048 +2050 4 433.6549 586.546 21.245 0.2897 2049 +2051 4 434.736 586.2863 21.0893 0.268 2050 +2052 4 435.8411 586.5574 20.925 0.3153 2051 +2053 4 436.9325 586.8731 20.739 0.3228 2052 +2054 4 438.0021 587.2335 20.5246 0.2676 2053 +2055 4 439.1324 587.2495 20.3143 0.2726 2054 +2056 4 440.2478 587.1168 20.1158 0.2176 2055 +2057 4 441.3632 587.3319 19.9072 0.1995 2056 +2058 4 442.4717 587.6099 19.7254 0.2155 2057 +2059 4 443.586 587.8387 19.5742 0.2337 2058 +2060 4 444.563 587.5801 19.434 0.2091 2059 +2061 4 444.9485 586.5288 19.2634 0.2172 2060 +2062 4 445.4553 585.6548 19.0585 0.201 2061 +2063 4 446.5181 585.2532 18.8479 0.2078 2062 +2064 4 447.5236 584.7659 18.6007 0.2703 2063 +2065 4 448.1814 583.9205 18.277 0.2583 2064 +2066 4 449.1573 584.0772 17.964 0.285 2065 +2067 4 450.2681 584.2545 17.6336 0.326 2066 +2068 4 451.2199 583.6745 17.3216 0.3288 2067 +2069 4 452.2312 583.1677 17.0075 0.3461 2068 +2070 4 453.358 583.3039 16.7418 0.3192 2069 +2071 4 454.4929 583.3016 16.4909 0.3263 2070 +2072 4 455.5397 582.8714 16.2484 0.2924 2071 +2073 4 456.4766 582.2296 15.755 0.2288 2072 +2074 4 390.8796 600.0269 31.1041 0.1797 2007 +2075 4 391.2308 598.9538 31.2757 0.1502 2074 +2076 4 391.5809 597.8807 31.512 0.1376 2075 +2077 4 392.0477 596.8385 31.7447 0.138 2076 +2078 4 392.5945 595.8341 31.9284 0.1384 2077 +2079 4 393.1436 594.8308 32.0589 0.1395 2078 +2080 4 393.4605 593.7326 32.1479 0.1414 2079 +2081 4 393.6458 592.6034 32.2151 0.1449 2080 +2082 4 393.83 591.4743 32.2776 0.1514 2081 +2083 4 394.0153 590.3452 32.349 0.1636 2082 +2084 4 393.6882 590.0992 32.2958 0.1144 2083 +2085 4 392.797 589.4266 32.755 0.1373 2084 +2086 4 391.9333 588.6864 32.9476 0.1373 2085 +2087 4 391.1004 587.9027 33.1142 0.1373 2086 +2088 4 390.2287 587.1626 33.2587 0.1373 2087 +2089 4 389.2357 586.6009 33.4006 0.1373 2088 +2090 4 388.2347 586.0518 33.5376 0.1373 2089 +2091 4 387.2326 585.5026 33.6664 0.1373 2090 +2092 4 386.1046 585.3482 33.7784 0.1373 2091 +2093 4 384.964 585.259 33.8792 0.1373 2092 +2094 4 383.8326 585.1045 33.9912 0.1373 2093 +2095 4 382.7092 584.9078 34.1236 0.1373 2094 +2096 4 381.6029 584.6332 34.2686 0.1373 2095 +2097 4 380.5928 584.1001 34.4103 0.1373 2096 +2098 4 379.5884 583.5555 34.5489 0.1373 2097 +2099 4 378.5839 583.011 34.6819 0.1373 2098 +2100 4 377.5783 582.4653 34.8062 0.1373 2099 +2101 4 376.5739 581.9208 34.9224 0.1373 2100 +2102 4 375.5695 581.3762 35.0336 0.1373 2101 +2103 4 374.5639 580.8317 35.1408 0.1373 2102 +2104 4 373.5595 580.2871 35.2439 0.1372 2103 +2105 4 372.5539 579.7426 35.343 0.1372 2104 +2106 4 371.6787 579.0093 35.4242 0.1371 2105 +2107 4 370.8379 578.2325 35.4903 0.1368 2106 +2108 4 369.9993 577.4546 35.5485 0.1364 2107 +2109 4 369.1608 576.6767 35.6135 0.1356 2108 +2110 4 368.2227 576.0234 35.686 0.1342 2109 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2131 +2133 4 402.41 570.4625 34.323 0.1589 2132 +2134 4 402.5221 569.3276 34.3101 0.1901 2133 +2135 4 402.6182 568.1905 34.2908 0.1732 2134 +2136 4 403.0037 567.162 34.3512 0.1621 2135 +2137 4 403.5506 566.1805 34.496 0.1571 2136 +2138 4 404.0711 565.168 34.6475 0.1867 2137 +2139 4 404.4566 564.095 34.7774 0.1669 2138 +2140 4 404.698 562.9784 34.886 0.15 2139 +2141 4 404.5664 561.8687 34.9706 0.1373 2140 +2142 4 404.4967 560.743 35.0431 0.1373 2141 +2143 4 404.6317 559.6105 35.1218 0.1373 2142 +2144 4 404.8033 558.4814 35.2139 0.1373 2143 +2145 4 404.9417 557.3476 35.3021 0.1373 2144 +2146 4 405.0526 556.2094 35.3718 0.1373 2145 +2147 4 405.1579 555.0699 35.4217 0.1373 2146 +2148 4 405.2631 553.9305 35.4547 0.1373 2147 +2149 4 405.3707 552.7922 35.4757 0.1373 2148 +2150 4 405.4816 551.6528 35.4914 0.1373 2149 +2151 4 405.5915 550.5145 35.5085 0.1373 2150 +2152 4 405.7253 549.3785 35.5309 0.1373 2151 +2153 4 406.0331 548.2837 35.567 0.1373 2152 +2154 4 406.4232 547.2084 35.6177 0.1373 2153 +2155 4 406.8133 546.1342 35.6782 0.1373 2154 +2156 4 407.2045 545.0588 35.7442 0.1373 2155 +2157 4 407.5946 543.9846 35.8117 0.1373 2156 +2158 4 407.9847 542.9092 35.8764 0.1373 2157 +2159 4 408.3874 541.8384 35.9293 0.1374 2158 +2160 4 408.8073 540.7745 35.9654 0.1375 2159 +2161 4 409.5772 539.9886 35.9876 0.1376 2160 +2162 4 410.5187 539.3388 35.9988 0.1379 2161 +2163 4 411.1044 538.4099 36.0024 0.1384 2162 +2164 4 411.4865 537.3322 36.0013 0.1393 2163 +2165 4 411.8572 536.25 35.9976 0.1411 2164 +2166 4 412.3525 535.2227 35.9923 0.1441 2165 +2167 4 412.8845 534.2102 35.9845 0.1506 2166 +2168 4 413.0218 533.1051 35.9736 0.1594 2167 +2169 4 412.9874 531.9611 35.959 0.1909 2168 +2170 4 412.9691 530.8171 35.9408 0.1748 2169 +2171 4 413.4874 529.8676 35.9078 0.1645 2170 +2172 4 414.2321 529.0016 35.8602 0.1646 2171 +2173 4 414.978 528.1345 35.8072 0.1859 2172 +2174 4 415.7227 527.2673 35.7585 0.2374 2173 +2175 4 416.4789 526.4105 35.7314 0.2744 2174 +2176 4 417.2465 525.5628 35.7389 0.2829 2175 +2177 4 418.0176 524.7173 35.7832 0.363 2176 +2178 4 418.6651 523.7861 35.894 0.4279 2177 +2179 4 419.1982 522.7897 36.0847 0.4967 2178 +2180 4 419.7553 521.8036 36.3278 0.5298 2179 +2181 4 420.4806 520.9296 36.5616 0.4785 2180 +2182 4 421.2643 520.0956 36.7688 0.3578 2181 +2183 4 421.667 519.082 37.0185 0.2585 2182 +2184 4 422.0319 518.0226 37.3024 0.2719 2183 +2185 4 422.7206 517.1338 37.5463 0.2523 2184 +2186 4 423.4756 516.2746 37.7476 0.2039 2185 +2187 4 424.0751 515.3102 37.9445 0.1713 2186 +2188 4 424.6253 514.3138 38.1391 0.1771 2187 +2189 4 425.1756 513.3174 38.3214 0.2094 2188 +2190 4 425.727 512.3198 38.4938 0.2793 2189 +2191 4 426.108 511.2479 38.6316 0.3596 2190 +2192 4 426.4443 510.1542 38.7282 0.405 2191 +2193 4 426.8722 509.0949 38.7881 0.4194 2192 +2194 4 427.3275 508.0458 38.8206 0.4418 2193 +2195 4 427.7828 506.9956 38.836 0.3902 2194 +2196 4 428.3365 505.9969 38.843 0.4034 2195 +2197 4 429.1533 505.2133 38.85 0.3574 2196 +2198 4 429.8786 504.3358 38.8604 0.2706 2197 +2199 4 430.0113 503.225 38.8746 0.2311 2198 +2200 4 430.0502 502.081 38.8945 0.2544 2199 +2201 4 430.6142 501.1132 38.9211 0.4013 2200 +2202 4 431.4116 500.2952 38.9575 0.3991 2201 +2203 4 431.7982 499.2256 39.0188 0.3553 2202 +2204 4 432.1609 498.1422 39.0995 0.2386 2203 +2205 4 432.5224 497.0588 39.1927 0.1946 2204 +2206 4 432.8953 495.9778 39.2907 0.191 2205 +2207 4 433.3278 494.9196 39.3739 0.2629 2206 +2208 4 433.7636 493.8614 39.4442 0.244 2207 +2209 4 434.1983 492.8032 39.5058 0.2611 2208 +2210 4 434.6434 491.7495 39.5685 0.2688 2209 +2211 4 435.1387 490.7211 39.6564 0.2844 2210 +2212 4 435.6375 489.6938 39.7681 0.3082 2211 +2213 4 436.1363 488.6665 39.8994 0.2621 2212 +2214 4 436.6316 487.6369 40.042 0.2716 2213 +2215 4 437.1258 486.6073 40.1892 0.2859 2214 +2216 4 437.6212 485.5777 40.3368 0.3288 2215 +2217 4 438.1142 484.5469 40.4799 0.3281 2216 +2218 4 438.6085 483.5173 40.6182 0.2587 2217 +2219 4 439.1027 482.4866 40.7518 0.2319 2218 +2220 4 439.5957 481.457 40.8811 0.2591 2219 +2221 4 440.1117 480.4365 41.0015 0.3945 2220 +2222 4 440.6882 479.4493 41.0998 0.4621 2221 +2223 4 441.2694 478.4631 41.1816 0.4479 2222 +2224 4 441.8677 477.4896 41.2709 0.4123 2223 +2225 4 442.4729 476.5218 41.3759 0.3922 2224 +2226 4 443.0792 475.5551 41.4952 0.3601 2225 +2227 4 443.5757 474.5255 41.6114 0.3898 2226 +2228 4 443.991 473.4593 41.7164 0.356 2227 +2229 4 444.4062 472.3931 41.8177 0.2658 2228 +2230 4 444.8307 471.3349 41.9406 0.2336 2229 +2231 4 445.2551 470.2755 42.0801 0.204 2230 +2232 4 445.6807 469.2173 42.231 0.2325 2231 +2233 4 446.1051 468.1591 42.3881 0.2231 2232 +2234 4 446.5295 467.1009 42.5435 0.2794 2233 +2235 4 446.9414 466.0336 42.6611 0.2545 2234 +2236 4 447.352 464.9651 42.7442 0.2631 2235 +2237 4 447.7627 463.8977 42.8039 0.3569 2236 +2238 4 447.8451 462.7594 42.8504 0.3826 2237 +2239 4 447.8314 461.6154 42.8932 0.3486 2238 +2240 4 447.8154 460.4714 42.9408 0.3382 2239 +2241 4 448.0236 459.348 43.0181 0.2927 2240 +2242 4 448.3919 458.2692 43.1315 0.335 2241 +2243 4 448.7626 457.1904 43.269 0.3713 2242 +2244 4 449.1333 456.1128 43.4199 0.2986 2243 +2245 4 449.5039 455.034 43.5733 0.273 2244 +2246 4 450.0519 454.0307 43.7035 0.2669 2245 +2247 4 450.7417 453.1178 43.7934 0.2833 2246 +2248 4 451.4316 452.2049 43.85 0.2941 2247 +2249 4 452.1225 451.2943 43.8819 0.2938 2248 +2250 4 452.722 450.3196 43.8976 0.3926 2249 +2251 4 453.2002 449.2808 43.9051 0.437 2250 +2252 4 453.6818 448.2432 43.9118 0.3919 2251 +2253 4 454.1897 447.2182 43.9208 0.3551 2252 +2254 4 454.6977 446.192 43.9334 0.4028 2253 +2255 4 455.2056 445.167 43.9516 0.3859 2254 +2256 4 455.7307 444.1511 43.9768 0.4084 2255 +2257 4 456.265 443.1398 44.011 0.4176 2256 +2258 4 456.7981 442.1274 44.0566 0.4781 2257 +2259 4 457.0086 441.2934 44.1294 0.3788 2258 +2260 4 457.2877 440.1849 44.2322 0.4204 2259 +2261 4 457.568 439.0775 44.361 0.3177 2260 +2262 4 457.8471 437.969 44.5105 0.3143 2261 +2263 4 458.1594 436.8753 44.6883 0.3148 2262 +2264 4 458.5701 435.8274 44.9268 0.3964 2263 +2265 4 459.0174 434.7944 45.1928 0.3861 2264 +2266 4 459.5368 433.7751 45.421 0.3503 2265 +2267 4 460.0699 432.7626 45.6056 0.2503 2266 +2268 4 460.5961 431.749 45.764 0.2278 2267 +2269 4 461.1018 430.7286 45.9281 0.1963 2268 +2270 4 461.6051 429.7081 46.0953 0.2029 2269 +2271 4 462.1085 428.6865 46.2664 0.243 2270 +2272 4 462.5203 427.6364 46.4302 0.2971 2271 +2273 4 462.6771 426.5038 46.5508 0.2658 2272 +2274 4 462.8864 425.3838 46.6309 0.2754 2273 +2275 4 463.2399 424.297 46.6763 0.3075 2274 +2276 4 463.5145 423.1862 46.6973 0.2971 2275 +2277 4 463.6792 422.0582 46.7037 0.2742 2276 +2278 4 463.8039 420.9325 46.7043 0.2498 2277 +2279 4 464.186 419.8537 46.7048 0.231 2278 +2280 4 464.5761 418.7795 46.706 0.1832 2279 +2281 4 464.8335 417.6698 46.7076 0.1564 2280 +2282 4 465.028 416.551 46.7096 0.1496 2281 +2283 4 465.3323 415.4528 46.7124 0.1575 2282 +2284 4 465.5748 414.3374 46.7166 0.1875 2283 +2285 4 465.8997 413.2403 46.7222 0.1685 2284 +2286 4 466.3173 412.1821 46.73 0.1528 2285 +2287 4 466.8447 411.1673 46.741 0.1425 2286 +2288 4 467.1421 410.084 46.7566 0.1469 2287 +2289 4 467.3846 408.9743 46.779 0.1556 2288 +2290 4 467.5539 407.8566 46.809 0.1692 2289 +2291 4 467.6958 406.7263 46.8465 0.2065 2290 +2292 4 468.1797 405.7196 46.905 0.2164 2291 +2293 4 468.5893 404.682 47.0128 0.1797 2292 +2294 4 468.7929 403.562 47.1282 0.1501 2293 +2295 4 469.2036 402.5084 47.2265 0.1374 2294 +2296 4 469.4518 401.4102 47.3119 0.1376 2295 +2297 4 469.8625 400.3622 47.3897 0.1379 2296 +2298 4 470.6153 399.5145 47.4639 0.1383 2297 +2299 4 470.9425 398.4586 47.542 0.1391 2298 +2300 4 470.931 397.3226 47.658 0.1407 2299 +2301 4 470.7915 396.2027 47.8416 0.1435 2300 +2302 4 470.7663 395.0735 48.0684 0.1494 2301 +2303 4 470.605 393.9444 48.2672 0.1571 2302 +2304 4 470.5432 392.8038 48.4327 0.1867 2303 +2305 4 470.5398 391.6781 48.6284 0.1669 2304 +2306 4 470.5512 390.5364 48.7794 0.15 2305 +2307 4 470.7217 389.4073 48.8824 0.1373 2306 +2308 4 471.5602 388.666 48.9552 0.1373 2307 +2309 4 471.8279 387.5746 49.0134 0.1373 2308 +2310 4 472.1883 386.4901 49.0613 0.1373 2309 +2311 4 472.4114 385.369 49.1016 0.1373 2310 +2312 4 472.5875 384.2387 49.1543 0.1373 2311 +2313 4 472.8141 383.137 49.2828 0.1373 2312 +2314 4 473.0291 382.0148 49.3954 0.1373 2313 +2315 4 473.5519 380.9989 49.4911 0.1373 2314 +2316 4 473.7361 379.8698 49.5726 0.1373 2315 +2317 4 473.9031 378.7384 49.6415 0.1373 2316 +2318 4 473.9741 377.5978 49.7036 0.1373 2317 +2319 4 474.0953 376.487 49.8319 0.1373 2318 +2320 4 474.5381 375.4368 49.938 0.1373 2319 +2321 4 474.657 374.2985 50.0251 0.1373 2320 +2322 4 474.7177 373.1591 50.0954 0.1373 2321 +2323 4 475.0266 372.0574 50.1528 0.1373 2322 +2324 4 475.3869 370.9717 50.2043 0.1373 2323 +2325 4 475.809 369.9101 50.2555 0.1373 2324 +2326 4 476.0939 368.8027 50.3244 0.1373 2325 +2327 4 476.2666 367.6724 50.4115 0.1373 2326 +2328 4 476.381 366.5479 50.5459 0.1373 2327 +2329 4 476.4874 365.4725 50.8001 0.1373 2328 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2350 +2352 4 479.6735 339.9865 52.953 0.1501 2351 +2353 4 480.003 338.8917 52.9774 0.1375 2352 +2354 4 480.3679 337.8106 53.0088 0.1377 2353 +2355 4 480.6001 336.7112 53.0474 0.1381 2354 +2356 4 480.8484 335.6324 53.1331 0.1388 2355 +2357 4 481.298 334.5925 53.2454 0.1399 2356 +2358 4 481.6309 333.5023 53.3355 0.1422 2357 +2359 4 481.7487 332.3709 53.4047 0.1463 2358 +2360 4 481.9192 331.2498 53.4565 0.1547 2359 +2361 4 482.3115 330.179 53.4943 0.1669 2360 +2362 4 482.76 329.1254 53.52 0.2053 2361 +2363 4 483.0517 328.026 53.543 0.1994 2362 +2364 4 483.1249 326.8888 53.5685 0.2202 2363 +2365 4 483.1249 325.7597 53.6469 0.2183 2364 +2366 4 483.1249 324.6237 53.7326 0.1832 2365 +2367 4 483.1341 323.4797 53.7855 0.1565 2366 +2368 4 483.2862 322.3506 53.8048 0.1499 2367 +2369 4 483.5768 321.2455 53.7911 0.1579 2368 +2370 4 483.9166 320.153 53.741 0.1883 2369 +2371 4 484.2197 319.0639 53.615 0.1698 2370 +2372 4 484.6545 318.0263 53.4344 0.1552 2371 +2373 4 484.9107 316.9269 53.2512 0.1471 2372 +2374 4 485.0526 315.7932 53.1042 0.1554 2373 +2375 4 485.5983 314.8185 53.0001 0.1718 2374 +2376 4 485.8179 313.7226 52.9348 0.1964 2375 +2377 4 485.6852 312.5923 52.904 0.2703 2376 +2378 4 486.0044 311.5181 52.8945 0.2702 2377 +2379 4 486.6393 310.5697 52.8934 0.2498 2378 +2380 4 487.0832 309.5195 52.8942 0.1953 2379 +2381 4 487.5396 308.4704 52.8951 0.1762 2380 +2382 4 487.9469 307.402 52.8962 0.1991 2381 +2383 4 488.2535 306.3003 52.8979 0.1873 2382 +2384 4 488.3816 305.1643 52.9001 0.2004 2383 +2385 4 488.4011 304.0214 52.9035 0.1687 2384 +2386 4 488.4091 302.8774 52.9082 0.1534 2385 +2387 4 488.6882 301.7712 52.9144 0.1436 2386 +2388 4 489.2648 300.7873 52.9231 0.1488 2387 +2389 4 489.5073 299.6731 52.936 0.1592 2388 +2390 4 489.9638 298.624 52.9544 0.1758 2389 +2391 4 490.0541 297.4846 52.9791 0.2188 2390 +2392 4 490.2177 296.3532 53.0102 0.2398 2391 +2393 4 490.2189 295.2092 53.0468 0.2209 2392 +2394 4 490.2189 294.0778 53.1474 0.2372 2393 +2395 4 490.2246 292.9349 53.2518 0.247 2394 +2396 4 490.2303 291.7909 53.3327 0.2489 2395 +2397 4 490.2372 290.6469 53.3918 0.2175 2396 +2398 4 490.2532 289.5052 53.4304 0.2153 2397 +2399 4 490.9076 288.5912 53.4509 0.2817 2398 +2400 4 491.0368 287.454 53.4551 0.2922 2399 +2401 4 491.1398 286.3157 53.4554 0.2854 2400 +2402 4 491.1467 285.1717 53.4556 0.286 2401 +2403 4 491.1535 284.0277 53.4559 0.2238 2402 +2404 4 491.1901 282.886 53.4568 0.187 2403 +2405 4 491.4475 281.7729 53.4576 0.1949 2404 +2406 4 491.8159 280.6907 53.4587 0.1828 2405 +2407 4 492.1042 279.5833 53.4604 0.1766 2406 +2408 4 492.3124 278.4588 53.4626 0.2 2407 +2409 4 492.492 277.3308 53.466 0.189 2408 +2410 4 492.7814 276.2245 53.4705 0.2036 2409 +2411 4 492.961 275.0977 53.4769 0.1747 2410 +2412 4 493.0137 273.9571 53.4856 0.1649 2411 +2413 4 493.2688 272.8532 53.4979 0.1622 2412 +2414 4 493.6509 271.8018 53.5156 0.1963 2413 +2415 4 493.652 270.6578 53.5405 0.1848 2414 +2416 4 493.652 269.5138 53.5738 0.1834 2415 +2417 4 493.7149 268.3755 53.6164 0.1973 2416 +2418 4 493.8808 267.251 53.6838 0.2572 2417 +2419 4 493.882 266.115 53.8 0.3184 2418 +2420 4 493.8888 264.9733 53.9269 0.3296 2419 +2421 4 493.8968 263.8293 54.0369 0.2746 2420 +2422 4 494.0147 262.707 54.1467 0.198 2421 +2423 4 494.5306 261.7152 54.2814 0.1606 2422 +2424 4 495.1804 260.7851 54.4015 0.1567 2423 +2425 4 495.4847 259.7086 54.4866 0.1745 2424 +2426 4 495.6918 258.5989 54.5476 0.2008 2425 +2427 4 496.0167 257.5052 54.5913 0.2817 2426 +2428 4 496.2603 256.3876 54.6213 0.2758 2427 +2429 4 496.3827 255.2573 54.644 0.334 2428 +2430 4 496.3919 254.1133 54.6694 0.3366 2429 +2431 4 496.5109 252.9796 54.7016 0.3957 2430 +2432 4 496.7156 251.855 54.7445 0.3544 2431 +2433 4 496.8838 250.7351 54.8358 0.3248 2432 +2434 4 497.2625 249.6849 54.9469 0.3851 2433 +2435 4 497.513 248.5981 55.0365 0.5089 2434 +2436 4 497.5839 247.4564 55.1065 0.452 2435 +2437 4 497.9878 246.421 55.16 0.3638 2436 +2438 4 498.2086 245.34 55.2006 0.2309 2437 +2439 4 498.2143 244.196 55.2325 0.1792 2438 +2440 4 498.22 243.052 55.265 0.1676 2439 +2441 4 498.2269 241.908 55.3039 0.1948 2440 +2442 4 498.3653 240.7926 55.4019 0.2376 2441 +2443 4 498.4614 239.6634 55.5134 0.3538 2442 +2444 4 498.8732 238.6304 55.6035 0.3909 2443 +2445 4 499.1158 237.5379 55.6741 0.2957 2444 +2446 4 499.1444 236.395 55.729 0.2281 2445 +2447 4 498.9808 235.2682 55.7715 0.2616 2446 +2448 4 498.9156 234.131 55.8048 0.2385 2447 +2449 4 499.022 232.9939 55.8401 0.266 2448 +2450 4 499.1455 231.8568 55.8894 0.2049 2449 +2451 4 499.5642 230.8237 55.9997 0.1778 2450 +2452 4 499.8044 229.7198 56.1081 0.1665 2451 +2453 4 500.1499 228.6318 56.1974 0.1859 2452 +2454 4 500.2632 227.5004 56.2691 0.2536 2453 +2455 4 500.2815 226.3564 56.3223 0.2263 2454 +2456 4 500.4897 225.2353 56.3595 0.2307 2455 +2457 4 501.3317 224.5283 56.4396 0.2008 2456 +2458 4 501.97 223.5879 56.5138 0.2155 2457 +2459 4 502.1176 222.4588 56.5463 0.2456 2458 +2460 4 502.4254 221.3732 56.4967 0.402 2459 +2461 4 503.0969 220.2086 56.4256 0.3186 2460 +2462 4 503.7718 219.2865 56.4074 0.2597 2461 +2463 4 504.3027 218.2741 56.4108 0.2575 2462 +2464 4 504.7694 217.2399 56.4469 0.3066 2463 +2465 4 505.4581 216.359 56.5636 0.3681 2464 +2466 4 505.9329 215.318 56.6591 0.3964 2465 +2467 4 506.4488 214.2975 56.7314 0.4075 2466 +2468 4 506.9258 213.2588 56.7818 0.4006 2467 +2469 4 507.2999 212.1788 56.8126 0.4061 2468 +2470 4 507.7049 211.1183 56.8252 0.3282 2469 +2471 4 508.2598 210.1231 56.8235 0.2679 2470 +2472 4 508.6418 209.0454 56.8204 0.205 2471 +2473 4 509.0754 208.0055 56.8162 0.1949 2472 +2474 4 509.636 207.0274 56.8106 0.2311 2473 +2475 4 510.0261 205.9566 56.8028 0.2604 2474 +2476 4 510.3601 204.8915 56.7916 0.2687 2475 +2477 4 510.5683 203.7876 56.7753 0.2787 2476 +2478 4 510.947 202.7145 56.7526 0.2096 2477 +2479 4 511.1781 201.5945 56.7235 0.1632 2478 +2480 4 511.4744 200.4929 56.6885 0.1383 2479 +2481 4 511.821 199.4141 56.6166 0.1391 2480 +2482 4 512.0727 198.3101 56.5065 0.1407 2481 +2483 4 512.202 197.1776 56.4147 0.1436 2482 +2484 4 512.4159 196.0759 56.345 0.1495 2483 +2485 4 512.75 194.9891 56.2954 0.1574 2484 +2486 4 513.1881 193.9583 56.2638 0.1874 2485 +2487 4 513.8127 193.0008 56.2478 0.1683 2486 +2488 4 514.3069 191.9746 56.2386 0.1525 2487 +2489 4 514.7142 190.9061 56.226 0.1419 2488 +2490 4 515.0402 189.8125 56.2092 0.1456 2489 +2491 4 515.1764 188.6811 56.1887 0.1534 2490 +2492 4 515.626 187.7007 56.1672 0.1644 2491 +2493 4 516.0836 186.7042 56.0966 0.2009 2492 +2494 4 516.5778 185.6987 56.016 0.1906 2493 +2495 4 517.1132 184.6908 55.9653 0.2066 2494 +2496 4 517.5124 183.6234 55.9628 0.1804 2495 +2497 4 517.9471 182.5744 56.0224 0.1747 2496 +2498 4 518.3258 181.5002 56.096 0.1846 2497 +2499 4 518.5615 180.3825 56.163 0.217 2498 +2500 4 518.8051 179.2659 56.2139 0.3244 2499 +2501 4 519.328 178.2615 56.2444 0.2934 2500 +2502 4 519.8027 177.2239 56.257 0.324 2501 +2503 4 520.0144 176.1062 56.2565 0.2962 2502 +2504 4 520.425 175.0492 56.2517 0.3114 2503 +2505 4 520.7191 173.9486 56.2456 0.2439 2504 +2506 4 521.2442 172.9431 56.2369 0.2407 2505 +2507 4 521.8436 171.9684 56.224 0.2147 2506 +2508 4 522.2131 170.893 56.2058 0.264 2507 +2509 4 522.7657 169.8977 56.1809 0.2251 2508 +2510 4 523.1329 168.8201 56.1498 0.214 2509 +2511 4 523.5859 167.7722 56.1126 0.2405 2510 +2512 4 524.1202 166.7769 56.0146 0.2894 2511 +2513 4 524.6853 165.785 55.9096 0.266 2512 +2514 4 525.0594 164.7074 55.8253 0.2057 2513 +2515 4 525.4243 163.6229 55.7623 0.1753 2514 +2516 4 525.6119 162.496 55.718 0.1813 2515 +2517 4 526.2434 161.558 55.6928 0.2326 2516 +2518 4 526.5523 160.4643 55.6808 0.2476 2517 +2519 4 526.9047 159.3764 55.6679 0.3209 2518 +2520 4 527.3279 158.3136 55.6478 0.3504 2519 +2521 4 527.9652 157.3664 55.6192 0.3499 2520 +2522 4 528.4639 156.3802 55.6802 0.2735 2521 +2523 4 528.2603 155.6481 55.5414 0.2059 2522 +2524 4 528.2203 154.5235 55.3983 0.2059 2523 +2525 4 528.3255 153.3841 55.2762 0.2059 2524 +2526 4 528.4399 152.2458 55.1718 0.2059 2525 +2527 4 528.4788 151.103 55.0822 0.2059 2526 +2528 4 528.8334 150.0196 55.0046 0.2059 2527 +2529 4 529.1275 148.9145 54.9534 0.2059 2528 +2530 4 529.3791 147.8082 54.8498 0.2059 2529 +2531 4 529.5816 146.686 54.7204 0.2059 2530 +2532 4 529.5828 145.542 54.6036 0.2059 2531 +2533 4 529.5153 144.4003 54.4961 0.2059 2532 +2534 4 529.3826 143.2643 54.3936 0.2059 2533 +2535 4 529.8184 142.2072 54.2942 0.2059 2534 +2536 4 530.2532 141.1559 54.1831 0.2059 2535 +2537 4 530.3355 140.0805 53.9409 0.2059 2536 +2538 4 530.4602 138.9571 53.7104 0.2059 2537 +2539 4 530.085 137.8817 53.5139 0.2059 2538 +2540 4 529.5999 136.8739 53.3476 0.2059 2539 +2541 4 529.5862 135.731 53.2 0.2059 2540 +2542 4 529.4581 134.6236 53.0309 0.2059 2541 +2543 4 529.0291 133.5792 52.8346 0.2059 2542 +2544 4 528.6264 132.5187 52.631 0.2059 2543 +2545 4 528.266 131.4319 52.4678 0.2059 2544 +2546 4 527.908 130.3462 52.3401 0.2059 2545 +2547 4 527.5579 129.2571 52.2441 0.2059 2546 +2548 4 527.1506 128.1932 52.169 0.2059 2547 +2549 4 526.4917 127.2849 52.0814 0.2059 2548 +2550 4 526.0112 126.4166 51.9683 0.2059 2549 +2551 4 526.4436 125.379 51.863 0.2059 2550 +2552 4 526.7525 124.3116 51.774 0.2059 2551 +2553 4 526.2503 123.3507 51.6984 0.2059 2552 +2554 4 525.5502 122.4469 51.634 0.2059 2553 +2555 4 525.2482 121.3761 51.5584 0.2059 2554 +2556 4 525.2882 120.2413 51.4567 0.2059 2555 +2557 4 525.3648 119.1018 51.3444 0.2059 2556 +2558 4 525.4209 117.959 51.2439 0.2059 2557 +2559 4 525.4712 116.8161 51.1549 0.2059 2558 +2560 4 525.2379 115.7064 51.0728 0.2059 2559 +2561 4 524.8821 114.6196 50.9944 0.2059 2560 +2562 4 524.3856 113.5906 50.9141 0.2059 2561 +2563 4 524.0572 112.5085 50.7822 0.2059 2562 +2564 4 523.9406 111.3983 50.5702 0.2059 2563 +2565 4 523.1546 110.6596 50.3524 0.2059 2564 +2566 4 522.1182 110.1773 50.1679 0.2059 2565 +2567 4 521.2956 109.3886 50.0119 0.2059 2566 +2568 4 520.6424 108.451 49.8781 0.2059 2567 +2569 4 520.5314 107.3139 49.7613 0.2059 2568 +2570 4 520.5211 106.1744 49.6138 0.2059 2569 +2571 4 520.5886 105.0469 49.3987 0.2059 2570 +2572 4 520.6584 103.9191 49.1296 0.2059 2571 +2573 4 520.806 102.7849 48.8866 0.2059 2572 +2574 4 520.9536 101.6503 48.6646 0.2059 2573 +2575 4 521.0634 100.5281 48.3907 0.2059 2574 +2576 4 521.1572 99.4198 48.0575 0.2059 2575 +2577 4 519.805 98.746 47.497 0.1373 2576 +2578 4 518.7948 98.2441 47.1797 0.1373 2577 +2579 4 518.0512 97.4488 46.858 0.1373 2578 +2580 4 517.819 96.3511 46.5867 0.1373 2579 +2581 4 517.0068 95.9996 46.2501 0.1373 2580 +2582 4 515.8754 95.9715 45.9276 0.1373 2581 +2583 4 514.8938 95.4061 45.6742 0.1373 2582 +2584 4 514.1491 94.546 45.4751 0.1373 2583 +2585 4 513.0543 94.2321 45.2766 0.1373 2584 +2586 4 511.9789 93.8757 45.0786 0.1373 2585 +2587 4 511.1975 93.04 44.9218 0.1373 2586 +2588 4 510.3773 92.2556 44.774 0.1373 2587 +2589 4 509.2619 92.0281 44.583 0.1373 2588 +2590 4 508.1831 91.697 44.3649 0.1373 2589 +2591 4 507.491 90.8002 44.1605 0.1373 2590 +2592 4 507.1947 89.7144 43.9356 0.1373 2591 +2593 4 507.1489 88.624 43.6052 0.1373 2592 +2594 4 506.7817 87.8001 43.2387 0.1373 2593 +2595 4 505.7075 87.6984 42.8442 0.1373 2594 +2596 4 504.6264 87.5546 42.5424 0.1373 2595 +2597 4 503.9274 86.7665 42.3186 0.1373 2596 +2598 4 503.67 85.6567 42.1627 0.1373 2597 +2599 4 503.463 84.5494 42.0596 0.1373 2598 +2600 4 503.7078 83.4525 41.9798 0.1373 2599 +2601 4 503.543 82.4515 41.8852 0.1373 2600 +2602 4 502.9287 81.4914 41.7511 0.1373 2601 +2603 4 502.3659 80.505 41.5778 0.1373 2602 +2604 4 502.0558 79.4291 41.3781 0.1373 2603 +2605 4 502.3613 78.3809 41.195 0.1373 2604 +2606 4 503.1587 77.5929 41.0399 0.1373 2605 +2607 4 503.5076 76.5984 40.9072 0.1373 2606 +2608 4 503.3863 75.4665 40.7873 0.1373 2607 +2609 4 503.2559 74.3305 40.668 0.1373 2608 +2610 4 503.1598 73.2383 40.4415 0.1373 2609 +2611 4 502.6725 72.3227 40.1646 0.1373 2610 +2612 4 502.073 71.3578 39.919 0.1373 2611 +2613 4 501.1772 70.7476 39.6889 0.1373 2612 +2614 4 500.7711 69.8282 39.3635 0.1373 2613 +2615 4 501.3077 68.886 39.0852 0.1373 2614 +2616 4 501.1189 67.7963 38.8724 0.1373 2615 +2617 4 500.9988 66.66 38.6954 0.1373 2616 +2618 4 501.2688 65.553 38.5314 0.1373 2617 +2619 4 502.184 64.9006 38.3729 0.1373 2618 +2620 4 502.9882 64.0932 38.1839 0.1373 2619 +2621 4 503.5705 63.1243 37.9212 0.1373 2620 +2622 4 504.3907 62.4316 37.5284 0.1373 2621 +2623 4 505.211 61.7384 37.0558 0.1373 2622 +2624 4 505.9832 60.9005 36.6506 0.1373 2623 +2625 4 506.7005 60.0097 36.3541 0.1373 2624 +2626 4 507.316 59.0453 36.1575 0.1373 2625 +2627 4 507.9955 58.1289 36.0455 0.1373 2626 +2628 4 508.9805 57.5519 35.9985 0.1373 2627 +2629 4 509.6051 56.6487 35.9836 0.1373 2628 +2630 4 508.9908 55.7008 35.9724 0.1373 2629 +2631 4 508.0355 55.0814 35.9565 0.1373 2630 +2632 4 506.9739 54.6546 35.9349 0.1373 2631 +2633 4 505.91 54.2344 35.9064 0.1373 2632 +2634 4 504.822 53.9061 35.8652 0.1373 2633 +2635 4 503.6826 53.8451 35.796 0.1373 2634 +2636 4 502.542 53.784 35.7084 0.1373 2635 +2637 4 501.4163 53.6546 35.6157 0.1373 2636 +2638 4 500.357 53.2247 35.5452 0.1373 2637 +2639 4 499.3297 52.723 35.4953 0.1373 2638 +2640 4 498.3195 52.1866 35.4645 0.1373 2639 +2641 4 497.2579 51.7814 35.4494 0.1373 2640 +2642 4 496.1242 51.6261 35.4435 0.1373 2641 +2643 4 494.9928 51.46 35.4413 0.1373 2642 +2644 4 493.8694 51.2439 35.4385 0.1373 2643 +2645 4 492.81 50.8852 35.4343 0.1373 2644 +2646 4 491.9795 50.0988 35.4284 0.1373 2645 +2647 4 491.1444 49.335 35.4203 0.1373 2646 +2648 4 490.0919 49.0734 35.4091 0.1373 2647 +2649 4 488.965 49.2179 35.3934 0.1373 2648 +2650 4 487.8371 49.0835 35.371 0.1373 2649 +2651 4 486.7606 48.7067 35.3396 0.1373 2650 +2652 4 485.7127 48.2516 35.296 0.1373 2651 +2653 4 484.6167 47.9426 35.2383 0.1373 2652 +2654 4 483.4841 47.7863 35.1551 0.1373 2653 +2655 4 482.3504 47.6811 35.0249 0.1373 2654 +2656 4 481.2179 47.6013 34.8485 0.1373 2655 +2657 4 480.0876 47.4911 34.6452 0.1373 2656 +2658 4 478.9791 47.2467 34.4448 0.1373 2657 +2659 4 477.9003 46.8703 34.2695 0.1373 2658 +2660 4 476.8203 46.494 34.1225 0.1373 2659 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481.7373 114.0019 61.8506 0.1716 3322 +3324 4 481.7304 112.8593 61.8332 0.1716 3323 +3325 4 481.0943 111.9184 61.8139 0.1716 3324 +3326 4 480.4079 111.006 61.7907 0.1716 3325 +3327 4 479.574 110.2562 61.7016 0.1716 3326 +3328 4 478.5832 109.7009 61.6328 0.1716 3327 +3329 4 477.8339 108.8421 61.6 0.1716 3328 +3330 4 476.9542 108.156 61.6557 0.1716 3329 +3331 4 475.944 107.6371 61.7095 0.1716 3330 +3332 4 475.0895 106.8781 61.7546 0.1716 3331 +3333 4 474.3207 106.0334 61.7873 0.1716 3332 +3334 4 473.6629 105.0991 61.7957 0.1716 3333 +3335 4 473.0554 104.1297 61.78 0.1716 3334 +3336 4 472.5189 103.13 61.7389 0.1716 3335 +3337 4 472.2283 102.0343 61.6599 0.1716 3336 +3338 4 471.9812 100.932 61.5507 0.1716 3337 +3339 4 471.4836 99.9125 61.4648 0.1716 3338 +3340 4 470.7343 99.0586 61.4009 0.1716 3339 +3341 4 469.9792 98.205 61.3572 0.1716 3340 +3342 4 469.517 97.182 61.3312 0.1716 3341 +3343 4 469.3924 96.054 61.32 0.1716 3342 +3344 4 469.3191 94.914 61.3152 0.1716 3343 +3345 4 468.9736 93.8407 61.3085 0.1716 3344 +3346 4 468.6373 92.757 61.2996 0.1716 3345 +3347 4 468.2232 91.7033 61.2864 0.1716 3346 +3348 4 467.8857 90.6228 61.2674 0.1716 3347 +3349 4 467.7416 89.4893 61.2416 0.1716 3348 +3350 4 467.2851 88.4786 61.2086 0.1716 3349 +3351 4 466.8138 87.4474 61.166 0.1716 3350 +3352 4 467.2451 86.5397 61.0778 0.1716 3351 +3353 4 466.911 85.7573 60.9608 0.1716 3352 +3354 4 465.8128 85.4464 60.8644 0.1716 3353 +3355 4 464.7855 84.9436 60.7858 0.1716 3354 +3356 4 463.8199 84.3344 60.7214 0.1716 3355 +3357 4 463.0432 83.5252 60.6665 0.1716 3356 +3358 4 462.6244 82.4609 60.6152 0.1716 3357 +3359 4 462.2138 81.3933 60.5545 0.1716 3358 +3360 4 461.787 80.336 60.4708 0.1716 3359 +3361 4 461.048 79.5467 60.3487 0.1716 3360 +3362 4 459.9555 79.2197 60.1745 0.1716 3361 +3363 4 458.863 78.9097 59.9463 0.1716 3362 +3364 4 457.7716 78.6522 59.642 0.1716 3363 +3365 4 456.8999 78.1697 59.2967 0.1716 3364 +3366 4 456.3954 77.1919 58.9649 0.1716 3365 +3367 4 455.4619 76.6028 58.6256 0.1716 3366 +3368 4 454.4563 76.1003 58.3167 0.1716 3367 +3369 4 453.5056 75.4807 58.0303 0.1716 3368 +3370 4 452.5035 75.4769 57.7808 0.1716 3369 +3371 4 451.4968 75.4404 57.5252 0.1716 3370 +3372 4 450.6822 74.697 57.2709 0.1716 3371 +3373 4 449.7625 74.2661 56.9828 0.1716 3372 +3374 4 448.6471 74.2639 56.6415 0.1716 3373 +3375 4 447.6861 73.7005 56.3399 0.1716 3374 +3376 4 447.86 72.5984 56.0784 0.1716 3375 +3377 4 448.1917 71.504 55.8438 0.1716 3376 +3378 4 447.312 70.9581 55.5453 0.1716 3377 +3379 4 446.4414 70.5473 55.1939 0.1716 3378 +3380 4 447.0043 69.7237 54.8484 0.1716 3379 +3381 4 447.6449 68.7976 54.4681 0.1716 3380 +3382 4 447.4813 67.7428 53.9851 0.1716 3381 +3383 4 447.0672 66.6851 53.5158 0.1716 3382 +3384 4 446.2 66.4203 52.9066 0.1716 3383 +3385 4 446.6096 65.5042 52.2099 0.1716 3384 +3386 4 447.0294 64.4746 51.5738 0.1716 3385 +3387 4 447.2571 63.3557 51.1006 0.1716 3386 +3388 4 447.1644 62.2708 50.7688 0.1716 3387 +3389 4 446.1817 61.73 50.5445 0.1716 3388 +3390 4 445.9255 60.7184 50.3986 0.1716 3389 +3391 4 446.3877 59.7289 50.2477 0.1716 3390 +3392 4 445.7653 58.9342 50.097 0.1716 3391 +3393 4 444.8524 58.2497 49.9498 0.1716 3392 +3394 4 444.0939 57.402 49.8 0.1716 3393 +3395 4 443.5494 56.4039 49.6437 0.1716 3394 +3396 4 442.7452 55.6897 49.429 0.1716 3395 +3397 4 441.6915 55.3832 49.1182 0.1716 3396 +3398 4 441.1138 54.4856 48.7654 0.1716 3397 +3399 4 440.6276 53.4674 48.3966 0.1716 3398 +3400 4 440.4308 52.3518 48.0679 0.1716 3399 +3401 4 440.5716 51.2223 47.8061 0.1716 3400 +3402 4 440.2901 50.1408 47.57 0.1716 3401 +3403 4 439.709 49.2197 47.35 0.1716 3402 +3404 4 439.701 48.1164 47.1635 0.1716 3403 +3405 4 439.0375 47.2094 46.963 0.1716 3404 +3406 4 438.1463 46.4952 46.7866 0.1716 3405 +3407 4 437.2951 45.7348 46.6385 0.1716 3406 +3408 4 436.3491 45.1013 46.492 0.1716 3407 +3409 4 435.324 44.6141 46.3434 0.1716 3408 +3410 4 434.2269 44.2922 46.2185 0.1716 3409 +3411 4 433.1287 43.9822 46.1208 0.1716 3410 +3412 4 432.1689 43.4164 46.0342 0.1716 3411 +3413 4 431.8875 42.449 45.9488 0.1716 3412 +3414 4 431.8177 41.5352 45.8576 0.1716 3413 +3415 4 430.7972 41.0586 45.752 0.1716 3414 +3416 4 429.7001 40.7477 45.6022 0.1716 3415 +3417 4 428.7998 40.1629 45.3438 0.1716 3416 +3418 4 427.9269 39.4905 45.001 0.1716 3417 +3419 4 426.8859 39.0652 44.6751 0.1716 3418 +3420 4 425.9501 38.4549 44.3993 0.1716 3419 +3421 4 425.1573 37.646 44.1224 0.1716 3420 +3422 4 424.6299 36.654 43.8656 0.1716 3421 +3423 4 424.1048 35.6607 43.591 0.1716 3422 +3424 4 423.0157 35.4713 43.349 0.1716 3423 +3425 4 421.8717 35.4497 43.1334 0.1716 3424 +3426 4 420.7438 35.3403 42.9248 0.1716 3425 +3427 4 419.657 35.3038 42.6244 0.1716 3426 +3428 4 419.7073 34.5747 42.3508 0.1716 3427 +3429 4 418.9877 33.9066 42.0563 0.1716 3428 +3430 4 417.9249 33.528 41.7105 0.1716 3429 +3431 4 416.8336 33.7917 41.3258 0.1716 3430 +3432 4 416.0545 32.9876 40.9108 0.1716 3431 +3433 4 416.0888 32.247 40.3844 0.1716 3432 +3434 4 415.7834 31.4862 39.7603 0.1716 3433 +3435 4 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65.984 0.183 3456 +3458 4 473.2739 115.6195 66.0694 0.183 3457 +3459 4 472.3061 115.0303 66.1606 0.183 3458 +3460 4 471.6472 114.1268 66.3368 0.183 3459 +3461 4 470.9093 113.28 66.5588 0.183 3460 +3462 4 470.2286 112.3616 66.7372 0.183 3461 +3463 4 469.5823 111.4307 66.8763 0.183 3462 +3464 4 468.6865 110.7207 66.981 0.183 3463 +3465 4 467.7324 110.0903 67.0552 0.183 3464 +3466 4 466.776 109.4643 67.1062 0.183 3465 +3467 4 465.8174 108.8693 67.1754 0.183 3466 +3468 4 464.8175 108.3784 67.291 0.183 3467 +3469 4 463.9069 107.6892 67.3837 0.183 3468 +3470 4 463.0397 106.9704 67.4548 0.183 3469 +3471 4 462.3064 106.0974 67.5069 0.183 3470 +3472 4 461.4702 105.3172 67.5427 0.183 3471 +3473 4 460.7449 104.4517 67.5657 0.183 3472 +3474 4 460.2621 103.4173 67.5836 0.183 3473 +3475 4 459.6764 102.4757 67.6074 0.183 3474 +3476 4 458.7062 101.9075 67.6362 0.183 3475 +3477 4 457.6332 101.5168 67.688 0.183 3476 +3478 4 456.5372 101.2324 67.7765 0.183 3477 +3479 4 455.407 101.078 67.8647 0.183 3478 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0.1487 3501 +3503 4 431.4379 89.8667 68.0557 0.1487 3502 +3504 4 430.525 89.1773 68.0442 0.1487 3503 +3505 4 429.6246 88.4717 68.028 0.1487 3504 +3506 4 428.7232 87.7663 68.0061 0.1487 3505 +3507 4 427.8331 87.0484 67.9767 0.1487 3506 +3508 4 427.0094 86.261 67.9311 0.1487 3507 +3509 4 426.2636 85.3958 67.863 0.1487 3508 +3510 4 425.5177 84.5304 67.7802 0.1487 3509 +3511 4 424.7077 83.7324 67.6953 0.1487 3510 +3512 4 423.7548 83.1077 67.6248 0.1487 3511 +3513 4 422.74 82.5801 67.5755 0.1487 3512 +3514 4 421.7905 81.9573 67.545 0.1487 3513 +3515 4 420.9485 81.1825 67.5298 0.1487 3514 +3516 4 420.1226 80.3911 67.5245 0.1487 3515 +3517 4 419.1971 79.7568 67.5242 0.1487 3516 +3518 4 418.1068 79.4197 67.5254 0.1487 3517 +3519 4 416.988 79.1831 67.5268 0.1487 3518 +3520 4 415.8566 79.0584 67.529 0.1487 3519 +3521 4 414.724 78.9518 67.5318 0.1487 3520 +3522 4 413.6704 78.5453 67.536 0.1487 3521 +3523 4 412.69 77.957 67.5419 0.1487 3522 +3524 4 411.7084 77.3684 67.55 0.1487 3523 +3525 4 410.7269 76.7802 67.5618 0.1487 3524 +3526 4 409.7465 76.1918 67.5774 0.1487 3525 +3527 4 408.7649 75.6033 67.5982 0.1487 3526 +3528 4 407.7525 75.0767 67.6301 0.1487 3527 +3529 4 406.6966 74.6383 67.6785 0.1487 3528 +3530 4 405.6327 74.2211 67.7404 0.1487 3529 +3531 4 404.5687 73.8038 67.8101 0.1487 3530 +3532 4 403.5037 73.3866 67.8818 0.1487 3531 +3533 4 402.4169 73.0395 67.9462 0.1487 3532 +3534 4 401.2946 72.8267 67.993 0.1487 3533 +3535 4 400.1609 72.6753 68.0184 0.1487 3534 +3536 4 399.0421 72.4504 68.0263 0.1487 3535 +3537 4 397.9484 72.1143 68.0187 0.1487 3536 +3538 4 396.8662 71.7438 67.9988 0.1487 3537 +3539 4 395.7862 71.3688 67.9675 0.1487 3538 +3540 4 394.7143 70.9706 67.9168 0.1487 3539 +3541 4 393.6515 70.5527 67.8426 0.1487 3540 +3542 4 392.5899 70.1345 67.7538 0.1487 3541 +3543 4 391.4882 69.8768 67.6684 0.1487 3542 +3544 4 390.3465 69.8164 67.6021 0.1487 3543 +3545 4 389.2037 69.7861 67.5559 0.1487 3544 +3546 4 388.062 69.7233 67.5273 0.1487 3545 +3547 4 386.9397 69.5258 67.5116 0.1487 3546 +3548 4 385.8346 69.2277 67.5027 0.1487 3547 +3549 4 384.7306 68.9294 67.4946 0.1487 3548 +3550 4 383.6267 68.6297 67.4836 0.1487 3549 +3551 4 382.5227 68.3277 67.4682 0.1487 3550 +3552 4 381.4199 68.0249 67.4475 0.1487 3551 +3553 4 380.3171 67.7221 67.4206 0.1487 3552 +3554 4 379.2234 67.3878 67.3789 0.1487 3553 +3555 4 378.1469 67.0087 67.3131 0.1487 3554 +3556 4 377.075 66.6161 67.228 0.1487 3555 +3557 4 375.9996 66.2294 67.142 0.1487 3556 +3558 4 374.9197 65.8513 67.0748 0.1487 3557 +3559 4 373.8398 65.4757 67.03 0.1487 3558 +3560 4 372.7587 65.1001 67.0071 0.1487 3559 +3561 4 371.6822 64.7146 67.0026 0.1487 3560 +3562 4 370.5828 64.4095 67.0124 0.1487 3561 +3563 4 369.4491 64.3064 67.0331 0.1487 3562 +3564 4 368.3051 64.3064 67.0634 0.1487 3563 +3565 4 367.1611 64.3064 67.1034 0.1487 3564 +3566 4 366.0171 64.3064 67.1552 0.1487 3565 +3567 4 364.8765 64.2946 67.2414 0.1487 3566 +3568 4 363.7508 64.1584 67.3767 0.1487 3567 +3569 4 362.6537 63.8513 67.5256 0.1487 3568 +3570 4 361.7637 63.1926 67.6656 0.1487 3569 +3571 4 361.1425 62.2479 67.8289 0.1487 3570 +3572 4 360.7844 61.1808 68.0252 0.1487 3571 +3573 4 360.5041 60.0845 68.2455 0.1487 3572 +3574 4 360.082 59.0297 68.4401 0.1487 3573 +3575 4 359.5363 58.0254 68.5952 0.1487 3574 +3576 4 359.3281 56.9268 68.721 0.1487 3575 +3577 4 359.311 55.7833 68.8223 0.1487 3576 +3578 4 359.232 54.6432 68.9144 0.1487 3577 +3579 4 358.7161 53.6767 69.0592 0.1487 3578 +3580 4 357.8855 52.9073 69.2093 0.1487 3579 +3581 4 356.9863 52.231 69.4092 0.1487 3580 +3582 4 356.0471 51.5879 69.5912 0.1487 3581 +3583 4 354.9557 51.28 69.7379 0.1487 3582 +3584 4 353.8358 51.0467 69.8561 0.1487 3583 +3585 4 352.7352 50.7385 69.951 0.1487 3584 +3586 4 351.9505 49.9331 70.0445 0.1487 3585 +3587 4 351.6725 48.8542 70.2002 0.1487 3586 +3588 4 350.9906 47.9461 70.3399 0.1487 3587 +3589 4 350.255 47.0995 70.5356 0.1487 3588 +3590 4 349.9496 45.9981 70.6899 0.1487 3589 +3591 4 349.9279 44.8543 70.8977 0.1487 3590 +3592 4 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63.4679 0.26 3703 +3705 4 509.0823 145.7525 63.5272 0.3185 3704 +3706 4 509.8042 144.8716 63.5667 0.2408 3705 +3707 4 510.6965 144.16 63.5888 0.1997 3706 +3708 4 511.3016 143.1991 63.5961 0.1951 3707 +3709 4 512.0041 142.2987 63.6014 0.1829 3708 +3710 4 512.8072 141.4842 63.609 0.1771 3709 +3711 4 513.5199 140.5907 63.6194 0.2001 3710 +3712 4 513.8516 139.4994 63.6336 0.1924 3711 +3713 4 514.6719 138.7123 63.6544 0.1943 3712 +3714 4 515.6328 138.0945 63.6843 0.2337 3713 +3715 4 516.5011 137.3498 63.7244 0.246 3714 +3716 4 517.4506 136.7114 63.7742 0.3358 3715 +3717 4 518.4265 136.1188 63.84 0.2911 3716 +3718 4 519.2124 135.3672 63.9996 0.3183 3717 +3719 4 520.3484 135.2494 64.1567 0.2926 3718 +3720 4 521.473 135.1762 64.3006 0.2707 3719 +3721 4 522.379 134.5687 64.3644 0.3339 3720 +3722 4 522.5117 133.7187 64.6582 0.2907 3721 +3723 4 523.3617 133.1868 64.9947 0.3026 3722 +3724 4 524.4062 132.7337 65.3033 0.3355 3723 +3725 4 525.3054 132.0393 65.5634 0.344 3724 +3726 4 526.1359 131.2546 65.7664 0.3861 3725 +3727 4 526.9596 130.4606 65.9151 0.3362 3726 +3728 4 527.7844 129.6667 66.003 0.2926 3727 +3729 4 528.8334 129.28 66.1097 0.318 3728 +3730 4 529.8367 128.7526 66.2435 0.4212 3729 +3731 4 530.0804 127.6578 66.3645 0.4542 3730 +3732 4 530.3378 126.5424 66.4756 0.4841 3731 +3733 4 530.991 125.6089 66.5795 0.4613 3732 +3734 4 531.6637 124.6823 66.6767 0.4562 3733 +3735 4 532.5514 123.9844 66.8242 0.495 3734 +3736 4 533.5719 123.5623 67.058 0.4475 3735 +3737 4 534.1862 122.5544 67.3907 0.3665 3736 +3738 4 534.7925 121.5843 67.4988 0.4113 3737 +3739 4 535.4435 120.644 67.5744 0.4635 3738 +3740 4 536.0395 119.667 67.622 0.3657 3739 +3741 4 536.353 118.5756 67.6567 0.3292 3740 +3742 4 536.2283 117.4545 67.7239 0.3593 3741 +3743 4 536.0258 116.3322 67.8185 0.3977 3742 +3744 4 536.0521 115.1951 67.912 0.439 3743 +3745 4 536.2706 114.0736 67.986 0.3176 3744 +3746 4 537.1629 113.445 68.0392 0.2523 3745 +3747 4 538.2268 113.0272 68.073 0.2708 3746 +3748 4 538.9087 112.13 68.0907 0.3138 3747 +3749 4 539.3045 111.0575 68.0985 0.4663 3748 +3750 4 539.7701 110.0128 68.1058 0.5107 3749 +3751 4 540.2597 108.9786 68.1159 0.4905 3750 +3752 4 541.0479 108.1507 68.1257 0.4935 3751 +3753 4 541.9185 107.4084 68.1321 0.4174 3752 +3754 4 542.7239 106.171 68.2472 0.3297 3753 +3755 4 543.2124 105.1422 68.28 0.2509 3754 +3756 4 543.3428 104.0468 68.166 0.27 3755 +3757 4 542.9069 102.9929 68.0355 0.3037 3756 +3758 4 542.9046 101.8509 67.891 0.3747 3757 +3759 4 543.0259 100.7133 67.732 0.3517 3758 +3760 4 544.3289 99.3851 67.1591 0.2577 3759 +3761 4 544.0532 98.5156 66.9032 0.2182 3760 +3762 4 543.09 98.1005 66.663 0.1781 3761 +3763 4 542.7067 97.0471 66.4149 0.1705 3762 +3764 4 542.6507 95.9299 66.1083 0.1769 3763 +3765 4 542.6987 94.8258 65.7258 0.2027 3764 +3766 4 542.6255 93.7176 65.2994 0.2969 3765 +3767 4 542.0512 92.8706 64.8824 0.2477 3766 +3768 4 541.0033 92.4248 64.4952 0.2127 3767 +3769 4 540.0492 91.8591 64.0934 0.2186 3768 +3770 4 539.6957 90.8963 63.7022 0.2281 3769 +3771 4 539.7998 89.7611 63.3587 0.254 3770 +3772 4 538.8984 89.1637 62.9894 0.3758 3771 +3773 4 538.1033 88.404 62.5932 0.357 3772 +3774 4 538.1662 87.3094 62.2098 0.2517 3773 +3775 4 537.3139 86.7423 61.8892 0.1706 3774 +3776 4 536.822 85.722 61.6025 0.1517 3775 +3777 4 536.1333 84.8184 61.3026 0.1652 3776 +3778 4 535.3085 84.0438 61.0033 0.1836 3777 +3779 4 534.8966 83.2147 60.7082 0.2492 3778 +3780 4 534.7079 82.2966 60.3562 0.2182 3779 +3781 4 533.6977 81.9301 59.8797 0.2154 3780 +3782 4 532.9827 82.4003 59.2256 0.1731 3781 +3783 4 533.1612 83.3279 58.5038 0.1612 3782 +3784 4 534.0329 83.5079 57.7867 0.1586 3783 +3785 4 534.7983 82.6607 57.1648 0.1746 3784 +3786 4 535.4972 81.7796 56.6272 0.2166 3785 +3787 4 535.4675 80.7654 56.1095 0.2349 3786 +3788 4 534.7697 79.9965 55.552 0.2152 3787 +3789 4 534.0741 79.2794 54.9637 0.2103 3788 +3790 4 533.6691 78.2196 54.4888 0.2758 3789 +3791 4 533.1326 77.3139 54.1108 0.2646 3790 +3792 4 532.0595 77.0501 53.79 0.3156 3791 +3793 4 531.3697 76.3839 53.5514 0.2901 3792 +3794 4 531.3846 75.2822 53.3924 0.3691 3793 +3795 4 532.1442 74.5855 53.2314 0.3599 3794 +3796 4 531.8994 73.9157 53.0813 0.2974 3795 +3797 4 530.9579 73.274 52.9673 0.2875 3796 +3798 4 529.8985 72.8577 52.883 0.3268 3797 +3799 4 528.8083 72.5116 52.817 0.3485 3798 +3800 4 527.7238 72.1492 52.7568 0.2948 3799 +3801 4 526.6553 71.7476 52.6865 0.3068 3800 +3802 4 525.5914 71.3326 52.5986 0.3605 3801 +3803 4 524.516 70.9492 52.5081 0.4213 3802 +3804 4 523.4235 70.6116 52.435 0.3783 3803 +3805 4 522.3264 70.2871 52.383 0.3689 3804 +3806 4 521.1961 70.1744 52.3508 0.3534 3805 +3807 4 520.0555 70.2435 52.3345 0.3156 3806 +3808 4 518.9161 70.3487 52.3289 0.2886 3807 +3809 4 517.7733 70.3571 52.3278 0.259 3808 +3810 4 516.6441 70.1974 52.3272 0.3334 3809 +3811 4 515.5184 69.9912 52.3264 0.3014 3810 +3812 4 514.3893 69.8091 52.3253 0.2655 3811 +3813 4 513.2567 69.6441 52.3236 0.2 3812 +3814 4 512.1242 69.4821 52.3214 0.1884 3813 +3815 4 510.9996 69.2779 52.3183 0.2044 3814 +3816 4 509.8934 68.9878 52.3141 0.282 3815 +3817 4 508.8112 68.6194 52.3079 0.307 3816 +3818 4 507.7587 68.1712 52.2995 0.244 3817 +3819 4 506.887 67.452 52.2878 0.2015 3818 +3820 4 506.0415 66.682 52.2707 0.219 3819 +3821 4 504.9982 66.2488 52.2472 0.2406 3820 +3822 4 503.9114 65.8898 52.2175 0.2193 3821 +3823 4 502.812 65.5755 52.1763 0.249 3822 +3824 4 501.6806 65.4856 52.0979 0.197 3823 +3825 4 500.5503 65.3515 51.9949 0.1634 3824 +3826 4 499.5631 64.8035 51.9084 0.1387 3825 +3827 4 498.5907 64.2001 51.8445 0.1397 3826 +3828 4 497.5954 63.6376 51.8011 0.1419 3827 +3829 4 496.5658 63.1396 51.7765 0.1456 3828 +3830 4 495.4515 62.9024 51.7675 0.1533 3829 +3831 4 494.3247 62.7082 51.7664 0.1645 3830 +3832 4 493.3065 62.2035 51.7664 0.2003 3831 +3833 4 492.2735 61.7119 51.7664 0.1931 3832 +3834 4 491.1718 61.4143 51.7664 0.1933 3833 +3835 4 490.061 61.1395 51.7664 0.2431 3834 +3836 4 489.3574 60.2742 51.7664 0.2102 3835 +3837 4 488.7385 59.3122 51.7664 0.1851 3836 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87.9606 73.5339 0.1373 3859 +3861 4 538.6135 86.8758 74.067 0.1373 3860 +3862 4 539.0963 85.9586 74.6094 0.1373 3861 +3863 4 539.3594 85.0477 75.1265 0.1373 3862 +3864 4 538.4362 84.4104 75.6316 0.1373 3863 +3865 4 537.4443 83.8784 76.1194 0.1373 3864 +3866 4 536.6801 83.0855 76.6326 0.1373 3865 +3867 4 535.9983 82.1899 77.117 0.1373 3866 +3868 4 535.2215 82.0087 77.5519 0.1373 3867 +3869 4 534.4379 82.8234 77.9951 0.1373 3868 +3870 4 533.5948 83.0125 78.5341 0.1373 3869 +3871 4 532.6521 82.6084 79.1622 0.1373 3870 +3872 4 531.5802 82.2676 79.7493 0.1373 3871 +3873 4 530.4922 81.9247 80.269 0.1373 3872 +3874 4 529.3837 81.8071 80.7184 0.1373 3873 +3875 4 528.4445 82.373 81.0984 0.1373 3874 +3876 4 527.5213 82.491 81.5738 0.1373 3875 +3877 4 526.844 81.7944 82.0996 0.1373 3876 +3878 4 526.8932 82.9131 82.6468 0.1373 3877 +3879 4 526.6862 83.9612 83.2098 0.1373 3878 +3880 4 525.6657 84.4335 83.7603 0.1373 3879 +3881 4 524.6819 84.7588 84.2864 0.1373 3880 +3882 4 524.0275 83.8513 84.7809 0.1373 3881 +3883 4 523.2679 83.1877 85.2404 0.1373 3882 +3884 4 522.2131 83.6217 85.6092 0.1373 3883 +3885 4 521.0977 83.7764 85.9468 0.1373 3884 +3886 4 519.988 83.5853 86.301 0.1373 3885 +3887 4 518.9905 83.1828 86.6536 0.1373 3886 +3888 4 518.2457 82.4505 86.9565 0.1373 3887 +3889 4 517.2459 82.8623 87.2998 0.1373 3888 +3890 4 516.246 82.863 87.761 0.1373 3889 +3891 4 515.4166 82.1329 88.1709 0.1373 3890 +3892 4 514.4076 81.6246 88.5408 0.1373 3891 +3893 4 513.3608 81.1731 88.8854 0.1373 3892 +3894 4 512.5257 80.4273 89.2382 0.1373 3893 +3895 4 512.0796 79.4129 89.614 0.1373 3894 +3896 4 511.6609 78.3823 90.0021 0.1373 3895 +3897 4 510.5729 78.2141 90.3272 0.1373 3896 +3898 4 509.4289 78.2151 90.5853 0.1373 3897 +3899 4 508.3044 78.0153 90.8054 0.1373 3898 +3900 4 507.2153 77.6768 91.0034 0.1373 3899 +3901 4 506.1273 77.3382 91.1826 0.1373 3900 +3902 4 505.0394 76.9993 91.3508 0.1373 3901 +3903 4 503.9514 76.6607 91.7168 0.1373 3902 +3904 4 542.6026 94.8369 67.4677 0.1602 3851 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145.9103 70.784 0.1144 1241 +1243 4 574.693 144.8201 70.6577 0.1144 1242 +1244 4 575.2204 143.8305 70.56 0.1144 1243 +1245 4 576.0509 143.0561 70.7482 0.1144 1244 +1246 4 576.9455 142.5424 71.6853 0.1144 1245 +1247 4 577.9351 142.1786 72.4679 0.1271 1246 +1248 4 578.8171 141.4991 72.6986 0.1525 1247 +1249 4 579.5069 140.5953 72.9848 0.178 1248 +1250 4 580.3501 139.9638 73.619 0.178 1249 +1251 4 581.3019 139.4193 74.2899 0.1525 1250 +1252 4 582.1633 138.6814 74.5898 0.1398 1251 +1253 4 583.0831 138.0328 74.9073 0.1398 1252 +1254 4 584.0681 137.4619 75.1097 0.1525 1253 +1255 4 585.1114 137.018 75.2102 0.1525 1254 +1256 4 586.181 136.6302 75.4656 0.1525 1255 +1257 4 587.1763 136.1509 76.1211 0.1525 1256 +1258 4 588.1842 135.7345 76.9266 0.1398 1257 +1259 4 589.2481 135.3489 77.2178 0.1271 1258 +1260 4 590.0912 134.6625 77.9108 0.1144 1259 +1261 4 590.7627 133.7485 78.1071 0.1144 1260 +1262 4 591.694 133.1044 78.12 0.1144 1261 +1263 4 592.203 132.1023 78.12 0.1144 1262 +1264 4 592.2545 130.9628 78.12 0.1144 1263 +1265 4 593.1285 130.2524 78.12 0.1398 1264 +1266 4 594.0792 129.6152 78.12 0.1907 1265 +1267 4 432.7855 379.5861 15.1883 0.3813 944 +1268 4 432.3794 378.5187 15.2043 0.3305 1267 +1269 4 431.9538 377.4639 15.4969 0.2924 1268 +1270 4 431.5832 376.4035 16.0068 0.2669 1269 +1271 4 431.2125 375.327 16.1736 0.2669 1270 +1272 4 430.859 374.2436 15.9807 0.2669 1271 +1273 4 430.4392 373.2003 15.5008 0.2415 1272 +1274 4 429.7344 372.3274 15.1698 0.2288 1273 +1275 4 428.7804 371.7188 15.0592 0.2161 1274 +1276 4 427.6821 371.4431 14.9531 0.2288 1275 +1277 4 426.5759 371.1639 15.073 0.2034 1276 +1278 4 425.743 370.5061 15.8942 0.1907 1277 +1279 4 424.9971 369.6619 16.2106 0.2034 1278 +1280 4 424.2787 368.7718 16.24 0.2796 1279 +1281 4 423.5534 367.8864 16.2397 0.3432 1280 +1282 4 422.7641 367.0593 16.238 0.3813 1281 +1283 4 421.9484 366.2573 16.2327 0.3686 1282 +1284 4 421.1053 365.484 16.2064 0.3432 1283 +1285 4 420.1649 364.8399 16.0611 0.3051 1284 +1286 4 419.1216 364.4349 15.573 0.2669 1285 +1287 4 418.2109 363.8469 14.747 0.2669 1286 +1288 4 417.6 362.9271 14.1554 0.2796 1287 +1289 4 416.9743 361.9742 14.0112 0.3051 1288 +1290 4 416.3325 361.027 14.0 0.2924 1289 +1291 4 415.542 360.2056 14.0 0.2924 1290 +1292 4 414.5925 359.5729 14.0 0.3178 1291 +1293 4 413.7116 358.8465 14.0 0.3559 1292 +1294 4 412.9474 357.9965 14.0008 0.3559 1293 +1295 4 412.2884 357.063 14.0042 0.2924 1294 +1296 4 411.7634 356.0483 14.0263 0.2161 1295 +1297 4 411.3275 354.9924 14.1529 0.1907 1296 +1298 4 410.7624 354.0097 14.4917 0.2161 1297 +1299 4 410.0782 353.0967 14.6748 0.2542 1298 +1300 4 409.4731 352.1404 14.3363 0.2542 1299 +1301 4 408.9571 351.1256 14.065 0.2415 1300 +1302 4 408.567 350.0526 14.0045 0.2161 1301 +1303 4 408.2192 348.9623 14.0022 0.1907 1302 +1304 4 408.0145 347.8378 14.0148 0.1525 1303 +1305 4 408.0991 346.7018 14.0997 0.1271 1304 +1306 4 408.3474 345.5864 14.2066 0.1144 1305 +1307 4 408.6528 344.4847 14.11 0.1144 1306 +1308 4 409.1836 343.4746 14.1131 0.1398 1307 +1309 4 409.3781 342.3637 14.2369 0.178 1308 +1310 4 408.8061 341.3788 13.9762 0.2288 1309 +1311 4 408.2055 340.4292 13.4532 0.2415 1310 +1312 4 407.6324 339.4408 13.2989 0.2542 1311 +1313 4 407.0444 338.4604 13.2255 0.2415 1312 +1314 4 406.4037 337.5223 12.9178 0.2542 1313 +1315 4 405.715 336.6083 12.8803 0.2542 1314 +1316 4 405.0366 335.6874 12.8822 0.2796 1315 +1317 4 404.3662 334.7607 12.8909 0.2796 1316 +1318 4 403.713 333.8226 12.9338 0.2669 1317 +1319 4 402.982 332.9463 13.1244 0.2288 1318 +1320 4 402.1915 332.141 13.5626 0.2034 1319 +1321 4 401.584 331.1869 13.7536 0.1907 1320 +1322 4 401.3381 330.0818 13.3683 0.2034 1321 +1323 4 401.155 328.9629 13.0029 0.2034 1322 +1324 4 400.9194 327.8441 12.9514 0.2034 1323 +1325 4 400.6322 326.7424 13.1916 0.1907 1324 +1326 4 400.2227 325.6934 13.6335 0.1907 1325 +1327 4 399.5843 324.7587 13.9412 0.178 1326 +1328 4 398.7275 324.0025 13.9969 0.1652 1327 +1329 4 397.794 323.3436 13.9838 0.178 1328 +1330 4 396.7987 322.7807 13.9121 0.2161 1329 +1331 4 395.7531 322.3403 13.627 0.2415 1330 +1332 4 394.7246 321.9399 13.1566 0.2288 1331 +1333 4 393.965 321.1231 13.4946 0.2415 1332 +1334 4 393.4388 320.1084 13.5055 0.2924 1333 +1335 4 392.8668 319.1371 13.1827 0.3559 1334 +1336 4 392.2513 318.2082 12.773 0.3432 1335 +1337 4 391.7125 317.2049 13.0141 0.2924 1336 +1338 4 391.2102 316.1959 13.1648 0.2288 1337 +1339 4 390.7675 315.1846 13.3658 0.2034 1338 +1340 4 390.2104 314.2396 14.1053 0.178 1339 +1341 4 389.7471 313.2066 14.2864 0.178 1340 +1342 4 389.4233 312.1656 13.65 0.1907 1341 +1343 4 389.2666 311.128 12.5566 0.2288 1342 +1344 4 389.0801 310.056 11.7642 0.2542 1343 +1345 4 388.8731 308.9566 11.2566 0.2415 1344 +1346 4 388.4784 307.9408 10.9614 0.2288 1345 +1347 4 387.7439 307.0656 10.8175 0.2288 1346 +1348 4 387.4122 306.0337 10.2435 0.2796 1347 +1349 4 386.6949 305.2329 10.4885 0.3305 1348 +1350 4 386.06 304.3074 10.2015 0.3559 1349 +1351 4 385.5497 303.3362 9.4259 0.3559 1350 +1352 4 385.1962 302.302 8.6097 0.3178 1351 +1353 4 384.8004 301.293 9.3607 0.2924 1352 +1354 4 384.1255 300.451 10.2819 0.2669 1353 +1355 4 383.24 299.728 10.36 0.2542 1354 +1356 4 433.4948 381.6235 18.1658 0.2924 943 +1357 4 434.1228 381.4668 20.2387 0.2034 1356 +1358 4 435.1067 381.4634 21.6325 0.1652 1357 +1359 4 436.1672 381.659 22.4507 0.178 1358 +1360 4 436.6911 382.4678 22.3471 0.2288 1359 +1361 4 436.7083 383.5981 22.1788 0.2542 1360 +1362 4 436.6362 384.7203 22.5845 0.2542 1361 +1363 4 436.6419 385.8449 23.0667 0.2415 1362 +1364 4 436.6934 386.9774 22.9566 0.2034 1363 +1365 4 437.0206 388.0288 22.3874 0.2034 1364 +1366 4 437.4542 389.0767 22.2429 0.2034 1365 +1367 4 438.0296 390.0422 21.9145 0.2542 1366 +1368 4 438.605 391.0181 22.008 0.2669 1367 +1369 4 439.2159 391.9813 22.1278 0.2924 1368 +1370 4 440.1883 392.495 22.0937 0.2669 1369 +1371 4 441.2946 392.7821 22.1236 0.2669 1370 +1372 4 442.2052 393.4319 22.2919 0.2542 1371 +1373 4 442.6628 394.4501 22.0623 0.2924 1372 +1374 4 442.8676 395.5712 21.9512 0.3178 1373 +1375 4 443.0678 396.6957 21.7955 0.3432 1374 +1376 4 443.578 397.6979 22.1746 0.3178 1375 +1377 4 444.1752 398.4712 20.8544 0.2924 1376 +1378 4 444.8215 399.4093 20.6668 0.2796 1377 +1379 4 445.5239 400.2353 21.56 0.3305 1378 +1380 4 446.2469 400.7832 21.866 0.2415 1379 +1381 4 447.2445 401.0967 22.9942 0.2542 1380 +1382 4 448.3073 401.4468 22.468 0.2415 1381 +1383 4 449.3438 401.8712 22.9359 0.2034 1382 +1384 4 450.45 401.8735 23.4702 0.1652 1383 +1385 4 451.4704 401.798 22.7223 0.1525 1384 +1386 4 452.3536 401.2237 22.8262 0.178 1385 +1387 4 453.3615 401.1001 23.6088 0.2034 1386 +1388 4 454.3076 401.5692 24.2589 0.2415 1387 +1389 4 455.1564 402.2487 25.0524 0.2669 1388 +1390 4 456.0762 402.8825 24.876 0.3051 1389 +1391 4 457.0074 403.4728 24.1343 0.3432 1390 +1392 4 457.8631 404.1638 23.4262 0.3432 1391 +1393 4 458.8138 404.6854 23.5626 0.3305 1392 +1394 4 459.6478 405.1979 24.885 0.2924 1393 +1395 4 460.4909 405.6372 26.4256 0.2669 1394 +1396 4 461.31 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0.2669 1504 +1506 2 411.0152 426.5496 8.2065 0.2288 1505 +1507 2 410.6914 427.6249 8.4067 0.2288 1506 +1508 2 410.4683 428.7243 8.9289 0.2034 1507 +1509 2 410.2933 429.8111 9.6664 0.2161 1508 +1510 2 410.0622 430.899 9.6303 0.2034 1509 +1511 2 409.8243 432.011 9.4004 0.2288 1510 +1512 2 409.0624 432.6619 9.9117 0.2415 1511 +1513 2 409.0944 433.576 10.92 0.2796 1512 +1514 3 420.0002 408.7546 10.0285 0.3051 1 +1515 3 421.0904 408.6276 9.3506 0.3686 1514 +1516 3 422.1371 408.2124 9.3579 0.4195 1515 +1517 3 423.0867 407.5866 9.5656 0.394 1516 +1518 3 424.0556 406.9952 9.8697 0.3432 1517 +1519 3 425.1138 406.5833 9.9075 0.2796 1518 +1520 3 426.2075 406.2676 9.6527 0.2161 1519 +1521 3 427.332 406.2401 9.4366 0.178 1520 +1522 3 428.4509 406.4277 9.1146 0.1652 1521 +1523 3 429.3329 406.6771 10.3457 0.178 1522 +1524 3 430.0079 407.1073 12.3208 0.178 1523 +1525 3 430.9288 407.169 13.7455 0.178 1524 +1526 3 431.765 406.4987 13.9502 0.1907 1525 +1527 3 432.4629 405.6029 13.6391 0.2415 1526 +1528 3 433.3735 404.9245 13.7175 0.2669 1527 +1529 3 434.1926 404.1558 14.2106 0.2669 1528 +1530 3 435.0655 403.4911 14.9923 0.2161 1529 +1531 3 435.864 403.3744 13.16 0.1652 1530 diff --git a/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Pvalb_469628681_m.swc b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Pvalb_469628681_m.swc new file mode 100644 index 00000000..4fe67b41 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Pvalb_469628681_m.swc @@ -0,0 +1,1250 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): K:/t301/production data/February 2015/169125.03.01.01/reconstructions/Pvalb-IRES-Cre; Ai14(IVSCC)-169125.03.01.01.DS.swc +# id,type,x,y,z,r,pid +1 1 312.0832 372.8296 27.44 5.1972 -1 +2 3 310.5926 376.7913 27.4061 0.2161 1 +3 3 310.5274 377.8643 27.3406 0.2796 2 +4 3 310.6829 378.9637 26.7414 0.2924 3 +5 3 310.6372 380.0368 25.7981 0.2542 4 +6 3 310.1784 380.9817 26.6462 0.2161 5 +7 3 309.7906 381.945 27.8124 0.1907 6 +8 3 309.6373 383.0558 27.412 0.1907 7 +9 3 308.9521 383.9264 26.7898 0.2288 8 +10 3 308.0803 384.6437 26.3326 0.2796 9 +11 3 307.3013 385.4811 26.2685 0.3686 10 +12 3 306.6515 386.418 26.0495 0.4576 11 +13 3 306.163 386.8493 25.4593 0.2542 12 +14 3 305.2078 387.4522 25.2165 0.2415 13 +15 3 304.1713 387.935 25.1997 0.2415 14 +16 3 303.1646 388.4772 25.1983 0.2034 15 +17 3 302.6612 389.4794 25.1899 0.2415 16 +18 3 302.0526 390.4449 25.1437 0.2669 17 +19 3 301.1488 391.113 24.7388 0.3051 18 +20 3 300.2908 391.8269 24.1321 0.2669 19 +21 3 299.3196 392.4252 24.0517 0.2542 20 +22 3 298.2831 392.9057 23.9078 0.2542 21 +23 3 297.186 392.9846 23.1896 0.3178 22 +24 3 296.0592 393.1093 22.8334 0.3178 23 +25 3 295.0021 393.4719 22.2421 0.3051 24 +26 3 293.9371 393.8781 22.4277 0.2415 25 +27 3 292.9361 394.3848 22.9662 0.2161 26 +28 3 292.4899 394.9992 22.9832 0.2161 27 +29 3 291.9008 395.9762 23.1025 0.2034 28 +30 3 291.2498 396.8708 23.7213 0.2288 29 +31 3 290.5634 397.7688 24.0411 0.2415 30 +32 3 289.8049 398.6234 24.0806 0.2669 31 +33 3 288.9069 399.3235 24.0828 0.2796 32 +34 3 288.05 400.0785 24.0904 0.2796 33 +35 3 287.2355 400.8816 24.1237 0.3051 34 +36 3 286.429 401.6904 24.2673 0.2924 35 +37 3 285.6545 402.4855 24.8928 0.2924 36 +38 3 284.9018 403.3321 25.1149 0.2415 37 +39 3 284.2554 404.2564 24.7358 0.2415 38 +40 3 283.5427 405.1282 24.2746 0.2669 39 +41 3 282.6424 405.7802 24.6834 0.3305 40 +42 3 281.8976 406.5742 25.4755 0.3432 41 +43 3 281.2192 407.4836 25.5206 0.3305 42 +44 3 280.3715 408.2387 25.2538 0.3051 43 +45 3 279.5433 409.0258 25.2843 0.3178 44 +46 3 278.7242 409.8083 25.6662 0.3178 45 +47 3 277.8204 410.4638 26.264 0.3051 46 +48 3 276.9144 411.1582 26.3071 0.2796 47 +49 3 275.99 411.832 26.2576 0.2669 48 +50 3 275.0862 412.523 25.9955 0.2796 49 +51 3 274.3198 413.3215 25.3011 0.3051 50 +52 3 273.7043 414.2813 25.2031 0.3432 51 +53 3 273.2352 415.3235 25.2 0.3813 52 +54 3 272.6312 416.2947 25.2 0.3813 53 +55 3 272.0638 417.2877 25.2 0.3686 54 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3 250.6882 432.5556 23.6606 0.2161 79 +81 3 249.8427 432.9193 24.5949 0.2415 80 +82 3 249.2787 433.8025 24.9186 0.2924 81 +83 3 248.7285 434.3962 23.8501 0.3178 82 +84 3 248.1485 435.3538 23.8171 0.2924 83 +85 3 247.668 436.1122 22.9415 0.2415 84 +86 3 247.0262 436.9714 22.675 0.1907 85 +87 3 246.2517 437.4587 24.2332 0.178 86 +88 3 245.5562 438.0468 24.8853 0.178 87 +89 3 245.134 438.883 24.1363 0.2034 88 +90 3 244.4739 439.6072 24.2189 0.2034 89 +91 3 243.7864 440.4022 23.6132 0.2034 90 +92 3 243.7098 441.2465 22.68 0.2034 91 +93 3 243.1275 441.8002 24.0089 0.2161 92 +94 3 242.9478 442.8413 23.6141 0.2161 93 +95 3 242.6138 443.8125 23.9828 0.178 94 +96 3 242.1299 444.6373 23.2663 0.1398 95 +97 3 241.2925 445.3638 23.4889 0.1271 96 +98 3 240.4253 445.4736 22.202 0.1398 97 +99 3 239.7824 446.0433 21.0 0.1525 98 +100 3 239.4335 446.732 21.884 0.1525 99 +101 3 238.818 447.4481 22.6209 0.1525 100 +102 3 237.7221 447.6506 22.398 0.1525 101 +103 3 236.9979 448.4366 21.6418 0.1398 102 +104 3 236.3058 449.3312 21.5261 0.1398 103 +105 3 235.6834 450.0702 21.7196 0.1398 104 +106 3 234.8907 450.8378 21.84 0.1652 105 +107 3 234.2695 451.3938 21.8378 0.1652 106 +108 3 233.6059 452.2896 21.56 0.1652 107 +109 3 232.7434 452.8227 21.56 0.1525 108 +110 3 231.835 453.4747 21.8126 0.178 109 +111 3 230.9301 454.0753 22.1673 0.2161 110 +112 3 229.9703 454.6027 21.7403 0.2542 111 +113 3 228.9716 454.8544 21.7193 0.2415 112 +114 3 227.9523 454.5226 22.12 0.2034 113 +115 3 227.0405 454.7366 22.96 0.1525 114 +116 3 226.3587 455.4573 22.96 0.1271 115 +117 3 225.5636 456.1849 22.96 0.1144 116 +118 3 224.6953 456.925 22.96 0.1271 117 +119 3 223.9014 457.5966 23.2285 0.1398 118 +120 3 223.5399 458.6731 23.24 0.1525 119 +121 3 222.8787 459.4556 22.965 0.1525 120 +122 3 221.8399 459.7736 23.5074 0.1652 121 +123 3 220.9453 460.2884 23.6846 0.178 122 +124 3 220.3905 461.2459 24.0321 0.178 123 +125 3 219.7624 461.7253 25.1622 0.1525 124 +126 3 219.7624 462.8521 25.4906 0.1271 125 +127 3 219.6011 463.94 26.1092 0.1144 126 +128 3 218.9627 464.798 26.5496 0.1144 127 +129 3 218.401 465.6538 27.594 0.1144 128 +130 3 217.7101 466.3127 28.0 0.1144 129 +131 3 217.1381 467.2794 28.1078 0.1525 130 +132 3 217.1312 468.3536 28.0 0.1907 131 +133 3 292.6455 393.8414 22.9662 0.2924 27 +134 3 292.0746 392.8542 22.9704 0.2924 133 +135 3 291.2876 392.0408 23.0171 0.2924 134 +136 3 290.2534 391.6084 23.2767 0.2669 135 +137 3 289.1517 391.4962 23.949 0.2669 136 +138 3 288.0489 391.2583 24.3723 0.2288 137 +139 3 286.9461 391.1222 25.0146 0.2288 138 +140 3 285.8227 391.0581 25.4668 0.2034 139 +141 3 284.7485 390.7835 26.0232 0.1907 140 +142 3 283.7189 390.3259 26.1685 0.1907 141 +143 3 282.5897 390.1784 26.0893 0.2161 142 +144 3 281.4812 389.985 26.3085 0.2542 143 +145 3 280.4322 389.5297 26.2489 0.2542 144 +146 3 279.3785 389.238 25.7748 0.2415 145 +147 3 278.3855 388.992 25.1258 0.2415 146 +148 3 277.515 388.3937 25.8048 0.2288 147 +149 3 276.5689 388.0963 26.8097 0.2288 148 +150 3 275.6582 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30.8286 0.4449 1049 +1051 3 314.7499 376.7741 26.964 0.1398 1 +1052 3 315.3905 377.7213 26.8498 0.178 1051 +1053 3 315.8264 378.5427 27.6531 0.2288 1052 +1054 3 316.9498 378.5324 27.44 0.2288 1053 +1055 3 317.5115 378.2064 29.1007 0.2288 1054 +1056 3 318.6543 378.2064 29.12 0.2288 1055 +1057 3 319.7903 378.3094 29.12 0.2924 1056 +1058 3 320.6632 378.2762 28.1529 0.3432 1057 +1059 3 321.3084 377.6104 28.7398 0.3686 1058 +1060 3 322.314 377.091 28.9512 0.3432 1059 +1061 3 323.3836 376.7192 28.9022 0.3051 1060 +1062 3 324.5196 376.3852 28.6958 0.2669 1061 +1063 3 325.341 376.6426 28.6843 0.2288 1062 +1064 3 325.9519 376.4424 29.4493 0.178 1063 +1065 3 327.0158 376.1472 29.4 0.1652 1064 +1066 3 327.7938 375.7274 28.28 0.1907 1065 +1067 3 328.5396 375.621 28.4964 0.2415 1066 +1068 3 329.3553 375.3624 29.5546 0.2669 1067 +1069 3 330.2305 375.5535 30.5978 0.2796 1068 +1070 3 331.1937 375.2675 31.8942 0.2669 1069 +1071 3 332.0037 375.0032 30.6956 0.2924 1070 +1072 3 332.9761 374.6886 30.0754 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390.0651 15.4031 0.2161 1182 +1184 3 429.8992 389.9896 16.3702 0.1907 1183 +1185 3 430.8304 390.2184 17.36 0.1907 1184 +1186 3 341.2243 372.5573 28.089 0.1652 1082 +1187 3 340.34 372.4098 28.9332 0.2288 1186 +1188 3 328.2433 375.4802 28.4889 0.2161 1066 +1189 3 328.7753 374.6692 29.5291 0.178 1188 +1190 3 328.6712 374.3557 31.3818 0.1907 1189 +1191 3 329.1345 373.476 31.92 0.2034 1190 +1192 3 329.2707 372.4509 32.2176 0.2669 1191 +1193 3 330.0108 371.7371 33.1324 0.2796 1192 +1194 3 330.9455 371.1182 33.32 0.2924 1193 +1195 3 331.4031 370.537 33.7296 0.2415 1194 +1196 3 331.4305 369.4994 33.847 0.2415 1195 +1197 3 331.4099 368.3897 33.7924 0.2415 1196 +1198 3 330.926 367.4837 34.44 0.2542 1197 +1199 3 330.6743 366.4026 34.839 0.2161 1198 +1200 3 330.7167 365.3375 35.5491 0.1652 1199 +1201 3 330.5016 364.3262 36.2132 0.1398 1200 +1202 3 330.0657 363.7188 35.0115 0.1398 1201 +1203 3 329.5269 362.8585 36.0828 0.1652 1202 +1204 3 329.1013 362.3483 38.2687 0.1907 1203 +1205 3 328.3646 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a/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Pvalb_470522102_m.swc b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Pvalb_470522102_m.swc new file mode 100644 index 00000000..c2700822 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Pvalb_470522102_m.swc @@ -0,0 +1,1966 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): K:/MouseCellTypes/176847.04.02.01/176847.04.02.01_MDF.swc_sorted.swc +# id,type,x,y,z,r,pid +1 1 237.4944 233.8336 35.28 5.9212 -1 +2 3 232.5043 230.7745 35.28 0.2669 1 +3 3 231.4495 231.199 35.28 0.2796 2 +4 3 230.3513 231.5181 35.28 0.2796 3 +5 3 229.2084 231.5159 35.28 0.2542 4 +6 3 228.0793 231.3282 35.28 0.2542 5 +7 3 226.9616 231.0846 35.28 0.2669 6 +8 3 225.8176 231.0617 35.28 0.3305 7 +9 3 224.6816 230.9313 35.2803 0.4576 8 +10 3 223.2768 230.103 35.2825 0.4068 9 +11 3 222.4268 229.3377 35.2898 0.3813 10 +12 3 221.523 228.6364 35.3265 0.3051 11 +13 3 220.8629 227.775 35.6944 0.4322 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+1868 3 326.9769 273.6276 19.88 0.1652 1867 +1869 3 328.0706 273.9617 19.88 0.1398 1868 +1870 3 329.1974 274.1024 19.88 0.1144 1869 +1871 3 330.3243 274.1745 20.1183 0.1144 1870 +1872 3 331.4019 274.3026 20.72 0.1144 1871 +1873 3 332.5276 274.1848 20.4565 0.1398 1872 +1874 3 333.5938 273.9891 19.88 0.1907 1873 +1875 3 334.7264 273.8736 19.7537 0.2669 1874 +1876 3 335.8452 273.9811 19.2934 0.2924 1875 +1877 3 336.9217 274.258 18.8513 0.2924 1876 +1878 3 338.0326 274.528 18.76 0.2542 1877 +1879 3 339.0576 274.981 18.5517 0.2415 1878 +1880 3 340.0574 275.5061 18.3386 0.2034 1879 +1881 3 341.0813 275.9923 18.2 0.178 1880 +1882 3 342.2127 276.0106 18.2 0.1652 1881 +1883 3 343.3384 275.8241 18.114 0.178 1882 +1884 3 344.4607 275.966 17.92 0.1907 1883 +1885 3 345.5486 276.3183 17.9068 0.1907 1884 +1886 3 346.5862 276.7565 17.64 0.1907 1885 +1887 3 347.5278 277.3994 17.5619 0.178 1886 +1888 3 348.5882 277.7632 17.36 0.1652 1887 +1889 3 349.7322 277.7632 17.36 0.1525 1888 +1890 3 350.8202 277.8902 16.8622 0.1525 1889 +1891 3 351.8967 278.2448 16.8 0.1525 1890 +1892 3 353.0373 278.3295 16.8 0.1525 1891 +1893 3 354.1721 278.3352 16.52 0.1525 1892 +1894 3 355.3058 278.2219 16.5508 0.1398 1893 +1895 3 356.4429 278.2208 16.8 0.1271 1894 +1896 3 357.5618 278.0675 17.057 0.1144 1895 +1897 3 358.7001 277.992 17.08 0.1144 1896 +1898 3 359.8383 277.9451 17.08 0.1271 1897 +1899 3 360.8233 277.5596 16.8179 0.1525 1898 +1900 3 361.5452 277.3056 18.5954 0.178 1899 +1901 3 362.6183 277.42 19.0509 0.178 1900 +1902 3 363.5998 277.3502 19.8408 0.1652 1901 +1903 3 364.7415 277.3056 19.88 0.1525 1902 +1904 3 365.8626 277.3056 20.2392 0.1525 1903 +1905 3 366.9826 277.3056 20.72 0.1398 1904 +1906 3 368.1266 277.3056 20.72 0.1271 1905 +1907 3 369.2706 277.309 20.72 0.1271 1906 +1908 3 370.402 277.436 20.72 0.1652 1907 +1909 3 371.5117 277.6911 20.72 0.2034 1908 +1910 3 372.4967 278.1728 20.72 0.2161 1909 +1911 3 373.4805 278.6304 20.72 0.178 1910 +1912 3 374.5262 278.9552 20.72 0.1398 1911 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283.712 16.6628 0.1525 1934 +1936 3 399.1588 283.5976 17.0492 0.1398 1935 +1937 3 400.265 283.4363 17.08 0.1271 1936 +1938 3 401.3793 283.2361 17.08 0.1271 1937 +1939 3 402.5187 283.1377 17.08 0.1398 1938 +1940 3 403.6432 283.0256 17.3687 0.1652 1939 +1941 3 404.7472 283.14 17.92 0.1652 1940 +1942 3 259.243 257.5876 23.8 0.2288 1804 +1943 3 259.3448 258.7236 23.8 0.2796 1942 +1944 3 259.3654 259.8619 23.5942 0.2669 1943 +1945 3 259.4569 260.999 23.52 0.2415 1944 +1946 3 259.7509 261.8959 22.96 0.2542 1945 +1947 3 260.5552 262.5572 22.12 0.2924 1946 +1948 3 261.2496 263.4655 22.12 0.3432 1947 +1949 3 261.936 264.3807 22.12 0.3432 1948 +1950 3 262.5549 265.3005 21.5533 0.3178 1949 +1951 3 263.0056 266.1894 20.4375 0.2669 1950 +1952 3 263.2207 267.1457 19.1559 0.2542 1951 +1953 3 264.0009 267.9591 19.04 0.2415 1952 +1954 3 264.7136 268.8354 18.7194 0.2288 1953 +1955 3 265.3405 269.5264 18.2 0.2161 1954 +1956 3 266.0498 270.2174 17.8942 0.2288 1955 +1957 3 266.7007 271.0113 17.36 0.2542 1956 +1958 3 267.4924 271.8258 17.36 0.2415 1957 +1959 3 268.1273 272.749 17.36 0.2034 1958 +1960 3 268.1536 273.8461 16.7597 0.1525 1959 +1961 3 268.1536 274.9741 16.2963 0.1271 1960 +1962 3 268.1925 276.0037 15.2967 0.1144 1961 +1963 3 269.2976 276.0472 15.12 0.1144 1962 diff --git a/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Rorb_325404214_m.swc b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Rorb_325404214_m.swc new file mode 100644 index 00000000..cd1d5859 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Rorb_325404214_m.swc @@ -0,0 +1,2194 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): C:/neuron tracing and reconstruction/vaa3d/vaa3d_bin_msvc_64bit_v2921_a/vaa3d_bin_msvc_64bit_v2921_a/new 168053.04.01.01.swc +# id,type,x,y,z,r,pid +1 1 415.6095 417.7339 47.8951 6.2366 -1 +2 4 413.4633 416.9525 48.2236 0.1144 1 +3 4 412.3903 416.5624 48.3879 0.1652 2 +4 4 411.3172 416.1712 48.552 0.2542 3 +5 4 410.2441 415.7811 48.7166 0.3813 4 +6 4 409.1001 415.7559 48.72 0.4576 5 +7 4 409.1871 414.7286 47.5728 0.2587 6 +8 4 408.9468 413.7368 46.8028 0.2501 7 +9 4 408.4984 412.7644 46.6435 0.2908 8 +10 4 408.6242 411.7382 45.6862 0.3458 9 +11 4 409.0727 410.9088 44.2305 0.3686 10 +12 4 409.8048 410.1068 43.4174 0.3514 11 +13 4 410.4535 409.2797 43.8572 0.3085 12 +14 4 411.0884 408.408 44.1526 0.2746 13 +15 4 411.6181 407.415 43.7422 0.2852 14 +16 4 412.0162 406.3522 43.4109 0.3197 15 +17 4 412.4715 405.3181 43.0052 0.3672 16 +18 4 413.0721 404.4749 41.925 0.4006 17 +19 4 413.5045 403.538 40.7725 0.3955 18 +20 4 413.9804 402.5095 40.8229 0.3338 19 +21 4 414.533 401.5509 40.1475 0.2932 20 +22 4 415.3326 400.9068 38.9242 0.3176 21 +23 4 416.3394 400.972 37.6132 0.572 22 +24 4 416.9869 400.3554 39.8726 0.3938 23 +25 4 417.7831 400.1895 41.337 0.3185 24 +26 4 418.8962 400.0431 41.5464 0.2855 25 +27 4 419.9006 399.5306 41.4324 0.2954 26 +28 4 420.7186 398.787 40.8402 0.3374 27 +29 4 420.6717 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277.8387 30.7269 0.3432 423 +425 4 225.0648 277.0894 30.4416 0.3708 424 +426 4 224.0375 276.6547 30.0476 0.4285 425 +427 4 222.9462 276.3401 30.0675 0.4576 426 +428 4 221.9028 275.9088 30.4612 0.4299 427 +429 4 220.9075 275.3619 30.7292 0.386 428 +430 4 219.9935 274.6836 30.7406 0.3756 429 +431 4 219.3174 273.7912 30.5004 0.3954 430 +432 4 218.9639 272.7319 30.0583 0.3926 431 +433 4 218.6756 271.6359 29.7497 0.3742 432 +434 4 218.1597 270.6292 29.68 0.3615 433 +435 4 217.3692 269.8204 29.68 0.3993 434 +436 4 216.375 269.2782 29.6797 0.4395 435 +437 4 215.358 268.7622 29.6786 0.4595 436 +438 4 214.4645 268.0586 29.6713 0.4555 437 +439 4 213.7633 267.1652 29.6344 0.4671 438 +440 4 213.102 266.2363 29.4742 0.4904 439 +441 4 212.363 265.3851 29.05 0.4957 440 +442 4 211.5565 264.5912 28.6854 0.4658 441 +443 4 210.7317 263.8007 28.5928 0.4149 442 +444 4 209.9251 262.9907 28.6689 0.3566 443 +445 4 209.1186 262.1876 28.9489 0.3154 444 +446 4 208.208 261.5184 29.2177 0.3051 445 +447 4 207.2264 260.9624 28.954 0.3281 446 +448 4 206.2678 260.3938 28.3296 0.3662 447 +449 4 205.316 259.8035 27.764 0.389 448 +450 4 204.387 259.1515 27.4935 0.4018 449 +451 4 203.5393 258.3873 27.4383 0.399 450 +452 4 202.7751 257.5373 27.4187 0.394 451 +453 4 202.0567 256.6484 27.3255 0.3784 452 +454 4 201.3028 255.8007 27.0217 0.3686 453 +455 4 200.4666 255.0342 26.6798 0.3686 454 +456 4 199.5388 254.3856 26.7848 0.3686 455 +457 4 198.6018 253.7381 27.0346 0.3445 456 +458 4 197.7427 253.007 26.7708 0.3062 457 +459 4 196.8904 252.2692 26.2962 0.276 458 +460 4 195.9317 251.6834 25.8591 0.2834 459 +461 4 194.9387 251.148 25.4038 0.3091 460 +462 4 194.099 250.3987 25.2151 0.3433 461 +463 4 193.4184 249.4824 25.1972 0.3559 462 +464 4 192.7949 248.5237 25.1854 0.3644 463 +465 4 192.1577 247.5742 25.1272 0.3772 464 +466 4 191.4129 246.715 24.9248 0.3986 465 +467 4 190.5675 245.9634 24.5428 0.4068 466 +468 4 189.7267 245.2015 24.2012 0.3981 467 +469 4 189.0025 244.3241 24.0878 0.3766 468 +470 4 188.4191 243.3425 24.0755 0.3599 469 +471 4 187.8334 242.361 24.0565 0.3383 470 +472 4 187.06 241.5304 23.9554 0.3305 471 +473 4 186.1368 240.8784 23.6239 0.3394 472 +474 4 185.1907 240.2823 23.0392 0.3521 473 +475 4 184.2687 239.6577 22.4062 0.347 474 +476 4 183.3454 239.0182 21.8744 0.3163 475 +477 4 182.3982 238.4016 21.4458 0.2961 476 +478 4 181.459 237.7621 21.1324 0.3016 477 +479 4 180.625 236.9922 21.2453 0.3327 478 +480 4 179.8185 236.1971 21.6258 0.3525 479 +481 4 178.941 235.4741 21.9047 0.3652 480 +482 4 177.9709 234.8872 22.2057 0.3592 481 +483 4 176.8818 234.6493 22.636 0.3463 482 +484 4 175.7459 234.6138 22.9093 0.3139 483 +485 4 174.6408 234.3587 22.972 0.2851 484 +486 4 173.6649 233.7787 23.0171 0.2898 485 +487 4 172.7863 233.0522 23.2008 0.3227 486 +488 4 171.9089 232.3372 23.5858 0.3709 487 +489 4 171.0383 231.6097 23.9509 0.4117 488 +490 4 170.2055 230.8306 24.1494 0.4297 489 +491 4 169.3967 230.0298 24.4166 0.422 490 +492 4 168.5947 229.2336 24.8441 0.3991 491 +493 4 167.7779 228.4408 25.132 0.3838 492 +494 4 166.9119 227.696 25.1961 0.3813 493 +495 4 165.9486 227.0829 25.2 0.3504 494 +496 4 164.9179 226.5886 25.1997 0.3226 495 +497 4 163.9386 226.0006 25.1994 0.2865 496 +498 4 163.1699 225.1644 25.1975 0.2902 497 +499 4 162.5075 224.2332 25.1877 0.2924 498 +500 4 161.7662 223.3637 25.1462 0.3137 499 +501 4 160.9551 222.5629 24.92 0.3391 500 +502 4 160.1474 221.7724 24.4986 0.3645 501 +503 4 159.3226 220.9899 24.1842 0.3792 502 +504 4 158.4005 220.3184 24.089 0.3813 503 +505 4 157.4052 219.7544 24.08 0.3813 504 +506 4 156.4763 219.0897 24.08 0.3704 505 +507 4 155.6309 218.321 24.08 0.3577 506 +508 4 154.7397 217.6048 24.08 0.3342 507 +509 4 153.7753 216.9905 24.08 0.3087 508 +510 4 152.7686 216.4482 24.0803 0.2831 509 +511 4 151.8202 215.811 24.0811 0.2796 510 +512 4 151.0194 214.9976 24.0853 0.3129 511 +513 4 150.2587 214.1431 24.1032 0.362 512 +514 4 149.4544 213.3308 24.1749 0.4019 513 +515 4 148.5964 212.5838 24.4712 0.4179 514 +516 4 147.727 211.8619 24.8931 0.4084 515 +517 4 146.9365 211.0428 25.1381 0.3621 516 +518 4 146.2307 210.1448 25.0807 0.2887 517 +519 4 145.4665 209.3154 24.6375 0.2345 518 +520 4 144.525 208.7308 23.9929 0.2288 519 +521 4 143.4782 208.2812 23.8067 0.2863 520 +522 4 142.4955 207.7001 23.9106 0.3387 521 +523 4 141.6775 206.9118 23.6776 0.3899 522 +524 4 140.9751 206.0264 23.2506 0.394 523 +525 4 140.3082 205.102 23.0317 0.3823 524 +526 4 139.6904 204.1399 23.0759 0.3578 525 +527 4 139.028 203.2236 23.4875 0.3441 526 +528 4 138.2261 202.4285 23.9173 0.3195 527 +529 4 137.2686 201.8085 24.0394 0.3059 528 +530 4 136.279 201.2365 23.9515 0.2932 529 +531 4 135.3638 200.5729 23.5306 0.3282 530 +532 4 134.5401 199.7859 23.3173 0.3305 531 +533 4 133.8194 198.8981 23.2876 0.3305 532 +534 4 133.1467 197.9898 22.8595 0.2823 533 +535 4 132.5736 197.0346 22.2306 0.2556 534 +536 4 131.8849 196.1308 21.9187 0.2058 535 +537 4 130.9319 195.505 21.9433 0.2034 536 +538 4 130.0019 194.8747 22.4608 0.2034 537 +539 4 129.3052 193.9835 22.8472 0.2651 538 +540 4 128.6371 193.0546 22.8894 0.2793 539 +541 4 128.2092 192.0021 22.5851 0.3169 540 +542 4 127.9427 190.9691 23.571 0.3178 541 +543 4 127.0492 190.309 24.2091 0.4576 542 +544 4 126.3559 189.8857 23.1672 0.3814 543 +545 4 125.5185 189.1124 22.9373 0.3559 544 +546 4 124.7212 188.2932 22.8298 0.3559 545 +547 4 123.9936 187.4318 22.3429 0.3559 546 +548 4 123.2568 186.6573 21.3503 0.3559 547 +549 4 122.3794 185.9606 20.7827 0.3559 548 +550 4 121.5603 185.1633 20.7211 0.394 549 +551 4 120.7561 184.3499 20.72 0.4449 550 +552 4 119.9678 183.5193 20.72 0.4322 551 +553 4 119.2826 182.6041 20.72 0.394 552 +554 4 118.5481 181.7267 20.72 0.3432 553 +555 4 117.7073 180.9511 20.72 0.3686 554 +556 4 116.7715 180.2933 20.72 0.3686 555 +557 4 115.9558 179.4913 20.72 0.3559 556 +558 4 115.3026 178.5521 20.72 0.3178 557 +559 4 114.7718 177.5385 20.72 0.3178 558 +560 4 113.9426 176.7503 20.7206 0.3305 559 +561 4 113.1704 176.2057 20.7239 0.3827 560 +562 4 112.2468 175.5308 20.7393 0.3836 561 +563 4 111.3422 174.8318 20.8261 0.3501 562 +564 4 110.4583 174.1122 21.063 0.312 563 +565 4 109.5664 173.4007 21.2691 0.2632 564 +566 4 108.6763 172.6857 21.4334 0.2017 565 +567 4 107.8196 171.9283 21.4046 0.1586 566 +568 4 106.9002 171.3209 20.7239 0.1416 567 +569 4 105.8424 170.9228 20.4058 0.1622 568 +570 4 104.7627 170.5464 20.3899 0.1765 569 +571 4 103.6407 170.3313 20.4075 0.2009 570 +572 4 102.516 170.194 20.7738 0.2264 571 +573 4 101.4505 169.8257 21.2038 0.2752 572 +574 4 100.4498 169.2754 21.1982 0.303 573 +575 4 99.4651 168.7068 20.8967 0.2934 574 +576 4 98.4155 168.2595 20.7446 0.2453 575 +577 4 97.289 168.0753 20.7354 0.2059 576 +578 4 96.1939 167.7482 20.8124 0.2034 577 +579 4 95.1674 167.2585 21.0865 0.1914 578 +580 4 94.0562 166.9988 20.9818 0.1786 579 +581 4 92.9713 166.6465 20.7757 0.1535 580 +582 4 92.0816 165.9326 20.778 0.1773 581 +583 4 91.1472 165.2863 21.0918 0.2274 582 +584 4 90.2014 164.6433 21.1851 0.2908 583 +585 4 89.3954 163.846 21.5404 0.3172 584 +586 4 88.3424 163.4101 21.7148 0.3178 585 +587 4 87.227 163.2374 21.2702 0.3052 586 +588 4 86.1868 162.7981 20.8211 0.3303 587 +589 4 85.2756 162.1082 20.7228 0.3557 588 +590 4 84.4582 161.3086 20.7175 0.3559 589 +591 4 83.696 160.4552 20.7091 0.2929 590 +592 4 83.4217 159.3455 20.6192 0.2035 591 +593 4 83.7408 158.3296 19.6 0.1144 592 +594 4 112.9716 175.9987 21.6857 0.3463 560 +595 4 112.3254 175.0755 21.84 0.294 594 +596 4 112.0351 173.9818 21.8406 0.2397 595 +597 4 111.7511 172.8744 21.8431 0.2185 596 +598 4 111.0932 171.9581 21.8627 0.2582 597 +599 4 110.3967 171.0532 22.0038 0.3204 598 +600 4 109.939 170.0224 22.3846 0.3521 599 +601 4 109.4909 168.9837 22.787 0.3559 600 +602 4 108.7492 168.1268 22.9446 0.3559 601 +603 4 107.7769 167.5342 22.9639 0.3783 602 +604 4 106.7066 167.1361 22.9849 0.3925 603 +605 4 105.6133 166.8055 23.1213 0.4053 604 +606 4 104.5667 166.3731 23.5024 0.3954 605 +607 4 103.6127 165.7645 23.8829 0.3827 606 +608 4 102.7241 165.0472 23.9728 0.3358 607 +609 4 101.7701 164.418 23.9137 0.2962 608 +610 4 100.76 163.8826 23.9557 0.2694 609 +611 4 99.829 163.2213 24.0495 0.2555 610 +612 4 98.9531 162.4858 24.0951 0.2312 611 +613 4 97.9642 161.916 24.1878 0.2172 612 +614 4 96.8823 161.5969 24.6061 0.2044 613 +615 4 95.7951 161.2857 25.0331 0.2034 614 +616 4 94.9175 160.5684 25.1849 0.1914 615 +617 4 94.1179 159.7504 25.1994 0.2027 616 +618 4 93.2906 158.9599 25.2 0.2154 617 +619 4 92.5872 158.0585 25.2 0.2161 618 +620 4 92.0293 157.0609 25.2006 0.204 619 +621 4 91.4189 156.0931 25.2025 0.1793 620 +622 4 90.652 155.2454 25.2106 0.1658 621 +623 4 89.7715 154.5166 25.2552 0.1409 622 +624 4 88.9659 153.733 25.7701 0.1277 623 +625 4 87.8592 153.7536 26.32 0.1144 624 +626 4 127.3878 189.7118 21.6006 0.2342 543 +627 4 127.7791 188.9911 20.5702 0.1568 626 +628 4 127.977 187.9134 21.2559 0.1217 627 +629 4 128.1051 186.8083 21.7804 0.1144 628 +630 4 128.6794 185.9137 21.8638 0.121 629 +631 4 129.6392 185.8794 21.0445 0.1349 630 +632 4 130.5315 186.4777 20.2124 0.1478 631 +633 4 131.4742 187.0943 19.9343 0.144 632 +634 4 132.4946 187.1309 20.6651 0.1303 633 +635 4 133.2954 186.512 21.8061 0.1173 634 +636 4 133.9818 185.6346 21.8011 0.1144 635 +637 4 134.8513 185.0283 20.8659 0.1144 636 +638 4 135.9129 184.6473 20.5862 0.1144 637 +639 4 136.6863 183.8316 20.683 0.1144 638 +640 4 137.145 182.7883 20.7085 0.1144 639 +641 4 137.2182 181.6523 20.6573 0.1144 640 +642 4 136.3385 180.9911 20.3974 0.1144 641 +643 4 135.4496 181.6672 20.72 0.1144 642 +644 4 346.4764 380.3869 43.5758 0.1526 280 +645 4 346.1401 379.5426 45.2712 0.1271 644 +646 4 345.5555 378.561 45.418 0.1145 645 +647 4 344.6986 377.8209 45.7789 0.1273 646 +648 4 343.6267 377.6069 46.5976 0.1401 647 +649 4 342.5639 377.3553 47.4032 0.1652 648 +650 4 341.7563 376.6631 48.4263 0.1657 649 +651 4 340.8926 375.9401 48.9006 0.1775 650 +652 4 339.8858 375.4539 49.4724 0.1652 651 +653 4 338.8448 375.2549 50.521 0.1646 652 +654 4 337.7809 375.2446 51.5494 0.1538 653 +655 4 336.7067 375.3018 52.498 0.1798 654 +656 4 335.6164 375.5054 53.1698 0.2175 655 +657 4 334.5182 375.82 53.2846 0.2408 656 +658 4 333.3879 375.971 53.3708 0.2259 657 +659 4 332.2542 375.8841 53.5382 0.1885 658 +660 4 331.1445 376.1586 53.6413 0.164 659 +661 4 330.0486 376.4652 53.7569 0.1525 660 +662 4 328.94 376.511 54.3987 0.1525 661 +663 4 327.8418 376.3199 55.0214 0.1506 662 +664 4 326.7184 376.2273 55.4316 0.1374 663 +665 4 325.5847 376.2078 55.2255 0.1246 664 +666 4 324.4853 376.3691 54.565 0.1144 665 +667 4 323.3916 376.6151 54.0952 0.1144 666 +668 4 322.3426 377.059 54.04 0.1178 667 +669 4 321.3324 377.5703 54.0921 0.1376 668 +670 4 320.3829 378.0165 54.5496 0.1834 669 +671 4 319.6828 378.7521 55.2241 0.2432 670 +672 4 318.5948 379.0701 55.1312 0.2669 671 +673 4 317.5755 379.5655 55.1396 0.2571 672 +674 4 316.7004 380.0677 53.9636 0.2542 673 +675 4 315.8458 380.7266 54.3424 0.2757 674 +676 4 315.1228 381.5995 54.5124 0.268 675 +677 4 314.2248 382.1658 53.5601 0.2551 676 +678 4 313.4377 382.9483 52.9217 0.2063 677 +679 4 312.4813 383.5695 52.8906 0.1668 678 +680 4 311.8544 384.0408 54.88 0.1144 679 +681 4 379.3618 398.12 45.3169 0.2171 202 +682 4 379.0438 397.0847 45.0506 0.2155 681 +683 4 379.204 396.0288 44.1857 0.2288 682 +684 4 379.7725 395.1582 43.4168 0.242 683 +685 4 380.5825 394.6011 42.2982 0.2474 684 +686 4 381.3993 394.1721 40.6588 0.2415 685 +687 4 382.3545 393.7099 39.8367 0.2347 686 +688 4 383.431 393.3495 39.5895 0.2219 687 +689 4 384.4595 392.9491 39.004 0.2092 688 +690 4 385.3999 392.4458 37.9982 0.2034 689 +691 4 386.3791 391.9493 37.3512 0.2242 690 +692 4 387.4007 391.4436 37.1232 0.2623 691 +693 4 388.3937 390.9014 36.7441 0.2796 692 +694 4 389.294 390.2344 36.2533 0.2655 693 +695 4 389.9873 389.3638 35.758 0.24 694 +696 4 390.5776 388.4029 35.299 0.2288 695 +697 4 391.3613 387.6158 34.8804 0.2288 696 +698 4 392.3371 387.0564 34.433 0.2215 697 +699 4 393.345 386.5301 34.1835 0.2161 698 +700 4 394.3299 385.9501 34.088 0.2087 699 +701 4 395.3492 385.4616 33.7744 0.2186 700 +702 4 396.3216 384.964 33.0114 0.2441 701 +703 4 397.2483 384.3794 32.2218 0.2931 702 +704 4 398.1486 383.6987 31.9332 0.3256 703 +705 4 399.0215 382.9597 31.9052 0.3227 704 +706 4 399.8188 382.144 31.8102 0.2858 705 +707 4 400.2341 381.1465 31.2612 0.2669 706 +708 4 400.3554 380.1272 30.0684 0.2669 707 +709 4 400.8393 379.1811 29.3429 0.2572 708 +710 4 401.5474 378.2911 29.3877 0.2345 709 +711 4 402.4878 377.6767 29.2006 0.2184 710 +712 4 403.5574 377.8335 28.6961 0.2384 711 +713 4 404.5756 378.3048 28.1753 0.2415 712 +714 4 405.6704 378.2339 27.5688 0.2534 713 +715 4 406.6325 377.6756 26.9805 0.2662 714 +716 4 407.5752 377.0532 26.5255 0.2909 715 +717 4 408.3783 376.2444 26.3754 0.3044 716 +718 4 409.0132 375.2972 26.5356 0.2809 717 +719 4 409.6881 374.3877 26.9416 0.2555 718 +720 4 410.3642 373.4702 26.7484 0.2421 719 +721 4 411.0804 372.5882 26.4272 0.278 720 +722 4 411.8411 371.7348 26.3301 0.3283 721 +723 4 412.5241 370.8173 26.3203 0.3792 722 +724 4 413.151 369.8609 26.3197 0.3934 723 +725 4 413.9667 369.0601 26.3189 0.3818 724 +726 4 414.9768 368.5259 26.3144 0.3445 725 +727 4 416.0591 368.1586 26.2828 0.3186 726 +728 4 417.0738 367.6358 26.1223 0.3302 727 +729 4 417.9432 366.9574 25.3898 0.3305 728 +730 4 418.3802 365.9656 24.5244 0.3305 729 +731 4 418.4272 364.9268 23.3587 0.293 730 +732 4 418.2281 363.8114 22.9908 0.2924 731 +733 4 418.3208 362.672 22.9393 0.2924 732 +734 4 418.7623 361.6184 22.8326 0.267 733 +735 4 419.2154 360.5934 22.265 0.229 734 +736 4 420.0676 359.8315 22.2432 0.1781 735 +737 4 421.1956 359.6782 22.4848 0.1652 736 +738 4 422.136 359.6736 24.08 0.1144 737 +739 4 380.0368 399.0386 46.48 0.4576 200 +740 4 392.4503 408.7638 48.701 0.4322 186 +741 4 391.5546 409.4754 48.6262 0.4068 740 +742 4 390.7446 410.2704 48.274 0.394 741 +743 4 390.1292 410.815 46.3336 0.2555 742 +744 4 389.5457 411.5094 44.688 0.1694 743 +745 4 389.1991 412.4726 43.5781 0.1525 744 +746 4 388.92 413.5411 42.8999 0.1674 745 +747 4 388.6465 414.6211 42.2834 0.178 746 +748 4 388.7552 415.669 41.421 0.1615 747 +749 4 389.3947 416.5498 40.8906 0.144 748 +750 4 390.0983 417.4364 40.5482 0.1578 749 +751 4 390.295 418.4924 39.94 0.2126 750 +752 4 390.0617 419.562 39.2101 0.2958 751 +753 4 389.8558 420.6591 38.6232 0.3664 752 +754 4 389.9679 421.7596 38.0198 0.4112 753 +755 4 390.3271 422.7846 37.1792 0.3796 754 +756 4 390.835 423.7765 36.5828 0.3281 755 +757 4 391.3246 424.8061 36.5327 0.2767 756 +758 4 391.3761 425.9284 36.4353 0.2669 757 +759 4 391.089 426.879 35.1607 0.2556 758 +760 4 390.7996 427.7336 33.4407 0.2428 759 +761 4 390.4518 428.7083 32.2736 0.2299 760 +762 4 390.3591 429.834 31.983 0.2407 761 +763 4 390.5433 430.9608 32.0734 0.2534 762 +764 4 390.8053 432.0739 32.0765 0.2781 763 +765 4 391.1645 433.1562 31.8752 0.2796 764 +766 4 391.4116 434.2601 31.4672 0.2676 765 +767 4 391.4871 435.3835 30.9809 0.2549 766 +768 4 391.5843 436.5172 30.686 0.2662 767 +769 4 391.7548 437.6212 30.091 0.3033 768 +770 4 391.7399 438.7492 29.636 0.3051 769 +771 4 391.5168 439.8463 30.1574 0.2683 770 +772 4 391.3052 440.9617 30.4965 0.2178 771 +773 4 391.3796 442.0736 29.8945 0.2161 772 +774 4 391.3166 443.1547 28.9971 0.2409 773 +775 4 390.8613 444.2003 28.8926 0.2541 774 +776 4 390.5994 445.302 29.2936 0.2416 775 +777 4 390.5319 446.4071 28.6017 0.2415 776 +778 4 390.342 447.5305 28.3536 0.2668 777 +779 4 389.8374 448.5544 28.5309 0.2924 778 +780 4 389.6132 449.6767 28.5538 0.3051 779 +781 4 389.3478 450.7886 28.5211 0.3051 780 +782 4 389.2586 451.9269 28.3212 0.3051 781 +783 4 389.5331 452.9931 27.5587 0.3051 782 +784 4 389.953 454.041 27.1152 0.2924 783 +785 4 390.096 455.1747 26.9539 0.2669 784 +786 4 390.0708 456.3096 27.2908 0.2288 785 +787 4 389.8741 457.4147 26.7512 0.2034 786 +788 4 389.6098 458.5152 27.1622 0.1907 787 +789 4 389.2243 459.8663 26.3494 0.2465 788 +790 4 388.8685 460.9096 25.5976 0.2748 789 +791 4 388.8856 462.0227 25.1664 0.3004 790 +792 4 389.2254 463.0981 24.7836 0.2947 791 +793 4 389.6384 464.1162 24.0285 0.2715 792 +794 4 389.9141 465.1859 23.315 0.235 793 +795 4 389.7768 466.3036 23.1669 0.218 794 +796 4 389.8386 467.4327 23.4486 0.238 795 +797 4 389.969 468.5584 23.1613 0.2747 796 +798 4 389.81 469.6806 22.9076 0.2796 797 +799 4 389.7688 470.8166 22.6212 0.235 798 +800 4 390.0033 471.9183 22.1945 0.1951 799 +801 4 390.0697 473.0406 22.58 0.1786 800 +802 4 389.3741 473.8711 23.3666 0.1902 801 +803 4 388.658 474.7497 23.5922 0.191 802 +804 4 388.5836 475.8834 23.7726 0.2034 803 +805 4 388.1775 476.9119 24.4751 0.2034 804 +806 4 387.6696 477.9106 24.9782 0.2034 805 +807 4 387.3881 479.0145 25.1429 0.2099 806 +808 4 387.2623 480.1482 25.0096 0.2481 807 +809 4 387.2749 481.2831 24.8349 0.2796 808 +810 4 387.5826 482.3813 24.9421 0.2699 809 +811 4 388.0459 483.4224 25.1451 0.2166 810 +812 4 388.6225 484.4096 25.2109 0.1578 811 +813 4 389.1659 485.4038 25.279 0.1246 812 +814 4 389.4187 486.5169 25.4892 0.1144 813 +815 4 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0.2942 2187 +2189 3 476.2323 455.6552 32.1608 0.2675 2188 +2190 3 477.3477 455.7868 31.6327 0.2163 2189 +2191 3 478.4208 456.1128 31.08 0.1144 2190 diff --git a/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Scnn1a_473845048_m.swc b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Scnn1a_473845048_m.swc new file mode 100644 index 00000000..84e566f9 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/morphologies/Scnn1a_473845048_m.swc @@ -0,0 +1,3786 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): K:/t301/production data/177300.01.02.01/reconstruction/Scnn1a-Tg3-Cre_Ai14_IVSCC_-177300.01.02.01___DS.swc +# id,type,x,y,z,r,pid +1 1 303.16 379.4648 28.56 5.4428 -1 +2 3 302.6646 375.232 23.2562 0.2524 1 +3 3 302.469 374.2356 21.9996 0.2615 2 +4 3 302.0057 373.2918 21.0081 0.3026 3 +5 3 301.2358 372.4956 20.7318 0.36 4 +6 3 300.3366 371.832 20.7847 0.3728 5 +7 3 299.7177 370.8768 20.9602 0.3575 6 +8 3 299.5049 369.798 21.7249 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390.6783 343.772 29.6909 0.1271 1069 +1071 3 391.693 343.6599 29.2886 0.1893 1070 +1072 3 392.5464 343.0055 28.3965 0.1271 1071 +1073 3 393.3919 342.3855 27.8029 0.2227 1072 +1074 3 394.2327 341.8409 27.4509 0.136 1073 +1075 3 395.3641 341.7082 27.3224 0.1144 1074 +1076 3 396.4807 341.7471 27.5232 0.1144 1075 +1077 3 397.5411 341.9896 27.7264 0.1306 1076 +1078 3 398.5399 342.2711 27.4305 0.2408 1077 +1079 3 399.5786 342.2631 27.0824 0.1652 1078 +1080 3 400.6357 342.3683 26.8537 0.1579 1079 +1081 3 401.4994 342.0457 26.0968 0.1866 1080 +1082 3 402.5233 341.6579 26.1246 0.1144 1081 +1083 3 403.4671 341.2541 26.4256 0.178 1082 +1084 3 404.42 340.809 26.1114 0.1907 1083 +1085 3 405.3089 340.4395 26.7702 0.1837 1084 +1086 3 406.0685 339.7794 26.7064 0.1661 1085 +1087 3 407.073 339.4111 26.88 0.1882 1086 +1088 3 408.0614 339.0519 27.4232 0.1144 1087 +1089 3 409.02 338.9294 26.8265 0.2616 1088 +1090 3 410.0016 339.0233 26.0212 0.157 1089 +1091 3 411.1044 339.1731 25.6119 0.1554 1090 +1092 3 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45.472 0.1144 1643 +1645 3 304.4756 322.2202 45.36 0.1922 1644 +1646 3 303.9516 321.4102 44.5449 0.1594 1645 +1647 3 303.7583 321.035 46.7432 0.1144 1646 +1648 3 302.7836 320.6884 46.9501 0.1271 1647 +1649 3 301.7483 320.2502 46.9535 0.1866 1648 +1650 3 301.0242 319.9699 47.9032 0.2034 1649 +1651 3 300.3721 319.2355 48.314 0.414 1650 +1652 3 300.0769 318.2345 48.0668 0.3417 1651 +1653 3 300.1284 317.1614 48.44 0.3773 1652 +1654 3 300.1387 316.1215 47.8092 0.2415 1653 +1655 3 300.7576 315.5278 48.7463 0.3264 1654 +1656 3 301.3113 314.9123 50.029 0.3082 1655 +1657 3 301.9016 314.8288 50.68 0.2161 1656 +1658 4 299.8081 376.1609 30.4601 0.3051 1 +1659 4 299.3013 375.1451 30.7992 0.3564 1658 +1660 4 298.8506 374.0937 30.7941 0.4198 1659 +1661 4 298.0509 373.2769 30.7602 0.4587 1660 +1662 4 297.2352 372.4795 30.5836 0.4971 1661 +1663 4 296.5694 371.6067 29.8116 0.5346 1662 +1664 4 295.8361 370.7304 29.6834 0.5439 1663 +1665 4 295.0902 369.8655 29.6951 0.4923 1664 +1666 4 294.5285 368.8702 29.7615 0.43 1665 +1667 4 294.0446 367.8429 30.109 0.3964 1666 +1668 4 293.6465 366.8076 30.7479 0.434 1667 +1669 4 293.5138 365.6727 30.8496 0.4585 1668 +1670 4 293.3433 364.5493 31.1094 0.4703 1669 +1671 4 293.1065 363.4968 32.027 0.4692 1670 +1672 4 293.0836 362.4203 32.928 0.453 1671 +1673 4 293.0436 361.2775 33.0453 0.4068 1672 +1674 4 292.7954 360.2307 33.073 0.3705 1673 +1675 4 292.6398 359.1005 33.2525 0.3928 1674 +1676 4 292.6226 357.9999 34.0102 0.4062 1675 +1677 4 292.5528 356.8628 34.1984 0.4312 1676 +1678 4 292.2382 355.7668 34.4064 0.4322 1677 +1679 4 292.0746 354.6789 35.1644 0.4198 1678 +1680 4 291.609 353.6379 35.2895 0.3568 1679 +1681 4 291.1148 352.606 35.3405 0.3054 1680 +1682 4 290.4753 351.6633 35.5897 0.2669 1681 +1683 4 289.8759 350.9495 36.5243 0.2694 1682 +1684 4 289.3016 350.0526 37.5057 0.2357 1683 +1685 4 288.6369 349.2335 37.863 0.2288 1684 +1686 4 287.7503 348.5574 37.2428 0.254 1685 +1687 4 286.9621 347.7703 36.9382 0.2927 1686 +1688 4 286.3077 346.8471 37.2775 0.312 1687 +1689 4 285.6614 345.9147 37.5234 0.3108 1688 +1690 4 284.9979 344.9835 37.5365 0.3121 1689 +1691 4 284.2691 344.106 37.5906 0.3389 1690 +1692 4 283.4946 343.2744 37.8624 0.3701 1691 +1693 4 282.8895 342.4335 38.8769 0.3885 1692 +1694 4 282.3609 341.5046 39.6357 0.3792 1693 +1695 4 281.9056 340.4624 39.6536 0.3612 1694 +1696 4 281.5373 339.4008 39.2008 0.3559 1695 +1697 4 281.0682 338.378 38.8234 0.3636 1696 +1698 4 280.4699 337.4171 39.0328 0.3764 1697 +1699 4 279.8247 336.4939 39.5248 0.3734 1698 +1700 4 279.1943 335.5489 39.7446 0.3607 1699 +1701 4 278.7859 334.4942 39.7603 0.3479 1700 +1702 4 278.2929 333.4771 39.7625 0.3512 1701 +1703 4 277.7197 332.4876 39.7743 0.364 1702 +1704 4 277.1443 331.4992 39.8362 0.3848 1703 +1705 4 276.6535 330.4844 40.2108 0.394 1704 +1706 4 276.2188 329.4468 40.7154 0.4025 1705 +1707 4 275.7921 328.3909 40.88 0.3814 1706 +1708 4 275.4134 327.3133 40.8806 0.3346 1707 +1709 4 275.132 326.2047 40.8836 0.2579 1708 +1710 4 275.0668 325.0699 40.9055 0.2029 1709 +1711 4 274.6664 324.0426 41.0228 0.1995 1710 +1712 4 273.9697 323.2418 41.8933 0.2402 1711 +1713 4 273.4377 322.314 42.7798 0.2954 1712 +1714 4 273.0614 321.2993 42.1565 0.3375 1713 +1715 4 272.6152 320.7524 41.9695 0.2288 1714 +1716 4 272.2548 319.6954 41.6987 0.3461 1715 +1717 4 271.6851 318.8751 41.6464 0.3855 1716 +1718 4 270.9999 318.175 42.4326 0.4599 1717 +1719 4 270.4393 317.2552 43.2054 0.3625 1718 +1720 4 270.0961 316.2336 43.7791 0.3183 1719 +1721 4 269.3662 315.5095 44.4444 0.2868 1720 +1722 4 269.2621 314.6458 43.127 0.3296 1721 +1723 4 268.4659 314.0692 42.4511 0.309 1722 +1724 4 267.863 313.1952 42.014 0.4068 1723 +1725 4 267.0188 312.5099 41.5904 0.3432 1724 +1726 4 266.4113 311.7343 42.0263 0.3988 1725 +1727 4 265.9251 310.9815 41.9614 0.3432 1726 +1728 4 265.7512 310.286 43.4372 0.3489 1727 +1729 4 265.3439 309.2873 43.12 0.3051 1728 +1730 4 265.2478 308.7073 42.3116 0.2998 1729 +1731 4 264.836 308.3892 41.0894 0.3316 1730 +1732 4 265.3748 307.5633 40.9175 0.2034 1731 +1733 4 265.1792 306.6675 40.1321 0.1144 1732 +1734 4 264.8474 306.258 40.0011 0.1652 1733 +1735 4 265.2501 305.4755 41.498 0.1813 1734 +1736 4 265.702 304.6049 42.56 0.317 1735 +1737 4 265.6505 303.7446 41.993 0.1675 1736 +1738 4 266.4708 303.343 41.6858 0.2187 1737 +1739 4 267.0519 302.6258 40.7162 0.2177 1738 +1740 4 267.8413 301.9966 41.0822 0.2161 1739 +1741 4 268.5288 301.3776 41.6612 0.2288 1740 +1742 4 268.5849 300.4373 41.6147 0.2875 1741 +1743 4 269.0631 299.5736 41.7836 0.4758 1742 +1744 4 269.3914 298.9936 40.8884 0.3212 1743 +1745 4 269.8318 298.3255 41.2056 0.4036 1744 +1746 4 270.2368 297.3084 40.6137 0.2486 1745 +1747 4 270.6246 296.3692 41.0295 0.3533 1746 +1748 4 270.858 295.3854 41.8037 0.2288 1747 +1749 4 271.4449 294.6418 42.6056 0.2717 1748 +1750 4 272.3452 294.1018 42.814 0.1144 1749 +1751 4 272.4539 293.6087 40.8814 0.1646 1750 +1752 4 272.7204 292.8034 41.979 0.2878 1751 +1753 4 272.8143 291.9248 40.7901 0.3051 1752 +1754 4 273.2318 291.2544 41.5232 0.2044 1753 +1755 4 273.416 290.282 42.1518 0.1271 1754 +1756 4 272.7925 289.8461 40.8962 0.198 1755 +1757 4 272.6209 288.9836 40.3808 0.2981 1756 +1758 4 272.5752 288.4493 39.2081 0.2321 1757 +1759 4 272.6678 287.5341 40.2833 0.1348 1758 +1760 4 272.7662 286.7596 38.92 0.2034 1759 +1761 4 273.0488 286.1064 40.0319 0.1923 1760 +1762 4 272.8818 285.1271 40.3096 0.2076 1761 +1763 4 272.9584 284.2691 39.5111 0.1652 1762 +1764 4 273.5498 283.6811 40.6347 0.1144 1763 +1765 4 274.1333 282.9146 39.6525 0.135 1764 +1766 4 274.2477 281.8381 39.0757 0.2477 1765 +1767 4 274.2385 280.9481 38.3975 0.2257 1766 +1768 4 273.8381 279.9242 38.4 0.1608 1767 +1769 4 273.3542 278.9278 38.1562 0.1745 1768 +1770 4 272.8348 278.0824 38.2018 0.1922 1769 +1771 4 272.3864 277.1775 38.519 0.2669 1770 +1772 4 272.1553 276.2691 37.8008 0.1528 1771 +1773 4 271.9288 275.1984 38.4185 0.1891 1772 +1774 4 272.3086 274.4124 38.0405 0.2547 1773 +1775 4 272.5752 273.4354 38.3214 0.2034 1774 +1776 4 272.3281 272.7582 38.0467 0.2834 1775 +1777 4 271.5982 272.2411 36.9163 0.2465 1776 +1778 4 271.1303 271.5684 38.2782 0.1144 1777 +1779 4 271.6863 270.8077 37.8616 0.2034 1778 +1780 4 271.875 269.9565 36.8329 0.2258 1779 +1781 4 271.6314 269.0722 37.3332 0.1783 1780 +1782 4 271.2413 268.3206 36.2782 0.2715 1781 +1783 4 271.2104 267.5198 36.0186 0.1965 1782 +1784 4 271.128 266.7373 36.9883 0.2924 1783 +1785 4 270.9244 265.726 36.4 0.2034 1784 +1786 4 270.8992 265.8656 37.24 0.2288 1785 +1787 4 271.1166 265.1277 36.12 0.1314 1785 +1788 4 271.2378 264.2434 36.2631 0.1144 1787 +1789 4 271.1177 263.1715 36.0511 0.1252 1788 +1790 4 270.9793 262.2403 37.1862 0.1181 1789 +1791 4 271.3225 261.2484 37.2089 0.1631 1790 +1792 4 271.2424 260.5483 36.1147 0.17 1791 +1793 4 271.128 259.8768 35.3027 0.2322 1792 +1794 4 271.1841 258.9398 36.1043 0.1841 1793 +1795 4 271.4712 257.9926 35.8907 0.1635 1794 +1796 4 271.5856 257.0682 35.5264 0.1904 1795 +1797 4 271.3831 256.065 34.7922 0.1447 1796 +1798 4 271.1406 254.9987 34.7516 0.2287 1797 +1799 4 271.128 254.0572 35.4824 0.2669 1798 +1800 4 271.0216 253.4772 34.2278 0.1983 1799 +1801 4 271.0948 252.5517 34.9602 0.1636 1800 +1802 4 271.3568 251.6811 35.3447 0.178 1801 +1803 4 271.8007 250.7545 35.2814 0.216 1802 +1804 4 271.9528 249.9285 34.2157 0.1446 1803 +1805 4 272.0306 249.0728 33.32 0.2889 1804 +1806 4 272.5408 248.5855 35.0 0.2669 1805 +1807 4 272.7296 247.5856 34.1986 0.1677 1806 +1808 4 272.518 246.564 34.7589 0.2193 1807 +1809 4 272.1233 245.4978 35.0 0.269 1808 +1810 4 271.8144 244.4476 35.0297 0.2444 1809 +1811 4 271.7023 243.338 35.3186 0.1659 1810 +1812 4 271.8888 242.5291 35.0 0.247 1811 +1813 4 272.5008 241.8313 35.5236 0.2798 1812 +1814 4 273.2558 241.1815 35.6112 0.2288 1813 +1815 4 273.8278 240.2755 36.2239 0.202 1814 +1816 4 274.1264 239.2516 36.0335 0.1725 1815 +1817 4 274.3266 238.1819 36.2085 0.1144 1816 +1818 4 274.6469 237.2564 35.1226 0.1695 1817 +1819 4 275.1514 236.4614 35.7316 0.1652 1818 +1820 4 275.704 235.7212 35.5776 0.1955 1819 +1821 4 275.7646 234.6676 35.6908 0.1967 1820 +1822 4 276.1353 233.7489 36.0923 0.2556 1821 +1823 4 276.276 232.7903 35.2531 0.2288 1822 +1824 4 276.7004 231.9666 34.5369 0.3238 1823 +1825 4 276.7462 230.9644 33.6 0.2204 1824 +1826 4 276.9933 230.2346 33.8254 0.2288 1825 +1827 4 277.857 229.7072 34.16 0.1144 1826 +1828 4 277.9989 228.6215 33.8125 0.2052 1827 +1829 4 278.6956 227.8299 33.6129 0.1306 1828 +1830 4 279.3431 227.1023 33.0711 0.1512 1829 +1831 4 279.2001 226.2066 33.8484 0.2924 1830 +1832 4 279.4792 225.8256 35.0 0.2415 1831 +1833 4 264.6175 308.5723 44.3792 0.3432 1729 +1834 4 264.0352 308.1101 43.7251 0.3051 1833 +1835 4 263.4106 307.3013 43.7478 0.4046 1834 +1836 4 263.0548 306.4787 43.4862 0.2063 1835 +1837 4 262.397 305.8793 43.9267 0.1913 1836 +1838 4 261.8593 305.4263 44.24 0.3159 1837 +1839 4 261.2919 304.5145 43.7203 0.405 1838 +1840 4 261.0288 303.5158 43.5868 0.3016 1839 +1841 4 261.0768 302.5445 43.787 0.2613 1840 +1842 4 260.7874 301.8112 42.4673 0.3777 1841 +1843 4 260.705 300.8548 41.2639 0.243 1842 +1844 4 260.2474 300.0071 41.8135 0.3305 1843 +1845 4 259.8985 299.3619 43.3874 0.3813 1844 +1846 4 259.1286 298.624 42.8565 0.2601 1845 +1847 4 258.655 298.0578 43.2172 0.2669 1846 +1848 4 258.1413 297.1712 43.1343 0.3247 1847 +1849 4 257.6288 296.6392 43.96 0.3686 1848 +1850 4 257.0648 295.7469 44.8678 0.276 1849 +1851 4 256.7239 294.8706 46.3028 0.1907 1850 +1852 4 256.2205 294.4965 45.6817 0.3671 1851 +1853 4 255.8956 293.5252 45.9388 0.3934 1852 +1854 4 255.7881 292.4098 45.4096 0.3386 1853 +1855 4 255.1692 291.7989 45.0251 0.486 1854 +1856 4 254.6544 291.2521 46.5273 0.3553 1855 +1857 4 254.3512 290.576 46.132 0.2844 1856 +1858 4 253.7392 289.9376 46.058 0.1661 1857 +1859 4 253.4326 289.2673 47.7621 0.275 1858 +1860 4 253.078 288.383 47.1654 0.2204 1859 +1861 4 252.7737 287.311 46.8532 0.2094 1860 +1862 4 252.3492 286.4862 47.0719 0.3208 1861 +1863 4 252.0426 285.7254 48.1449 0.2672 1862 +1864 4 251.9752 284.9143 49.3147 0.2288 1863 +1865 4 251.2064 284.2794 50.3468 0.2288 1864 +1866 4 250.5543 283.4043 50.0458 0.3202 1865 +1867 4 249.9937 282.997 48.16 0.2812 1866 +1868 4 249.4229 282.4628 49.287 0.3432 1867 +1869 4 249.1254 281.551 49.9022 0.2796 1868 +1870 4 248.5626 281.6242 48.8846 0.1683 1869 +1871 4 247.4918 281.4274 49.5628 0.2463 1870 +1872 4 246.7505 280.7982 49.3511 0.2515 1871 +1873 4 245.8525 280.3807 50.1315 0.281 1872 +1874 4 245.2816 279.8853 50.1273 0.2169 1873 +1875 4 244.5666 279.454 49.28 0.3051 1874 +1876 4 243.6812 278.7917 49.6138 0.2918 1875 +1877 4 243.0119 278.1613 50.6066 0.3382 1876 +1878 4 242.3106 277.4028 51.2865 0.2942 1877 +1879 4 241.9754 276.657 50.4053 0.2393 1878 +1880 4 241.4755 275.7978 50.8094 0.2587 1879 +1881 4 240.7239 275.1034 51.8507 0.178 1880 +1882 4 240.2343 274.2065 52.0618 0.2288 1881 +1883 4 239.7103 273.3828 52.1007 0.2203 1882 +1884 4 239.5833 272.4505 53.1185 0.3305 1883 +1885 4 238.9473 271.9196 52.7853 0.2432 1884 +1886 4 238.5938 271.1852 53.6505 0.1971 1885 +1887 4 237.8342 270.421 53.9773 0.1652 1886 +1888 4 237.0288 270.3855 54.3074 0.2339 1887 +1889 4 236.3229 269.7529 53.5836 0.2843 1888 +1890 4 235.672 269.0185 54.2688 0.2011 1889 +1891 4 235.0623 268.2451 53.7323 0.2161 1890 +1892 4 234.568 267.3791 53.5648 0.2474 1891 +1893 4 234.1368 266.6767 54.0492 0.211 1892 +1894 4 234.0464 266.4273 56.2489 0.229 1893 +1895 4 233.7192 265.3314 56.2489 0.2516 1894 +1896 4 232.8463 264.6518 56.0311 0.2193 1895 +1897 4 232.2423 264.0752 54.9332 0.217 1896 +1898 4 231.7206 263.2699 54.9489 0.2288 1897 +1899 4 231.0102 262.9015 55.5892 0.2543 1898 +1900 4 230.794 262.2483 55.9908 0.2587 1899 +1901 4 230.6007 261.3857 56.5454 0.3432 1900 +1902 4 230.0641 260.4819 55.5845 0.2424 1901 +1903 4 229.8296 259.6251 54.5714 0.4277 1902 +1904 4 229.5962 258.7854 54.6501 0.2934 1903 +1905 4 228.8092 258.3598 55.8144 0.3215 1904 +1906 4 228.5026 257.4057 56.4029 0.2382 1905 +1907 4 228.5884 256.5981 56.84 0.1876 1906 +1908 4 228.2589 255.5055 56.8851 0.1709 1907 +1909 4 227.5942 254.7322 57.3448 0.1963 1908 +1910 4 226.8598 254.1671 56.6222 0.2669 1909 +1911 4 226.1665 253.3377 56.9898 0.1986 1910 +1912 4 225.9045 252.379 57.12 0.1984 1911 +1913 4 225.3989 251.3608 57.3017 0.2666 1912 +1914 4 225.1449 250.7328 58.3472 0.2994 1913 +1915 4 224.6438 250.1505 59.6285 0.2255 1914 +1916 4 224.621 249.2078 59.0848 0.3197 1915 +1917 4 223.9094 248.5718 59.64 0.2767 1916 +1918 4 223.8808 247.9574 59.8441 0.3122 1917 +1919 4 223.0125 247.5994 59.3158 0.2549 1918 +1920 4 222.9656 246.6464 60.48 0.3051 1919 +1921 4 222.2849 246.1327 61.6753 0.1889 1920 +1922 4 221.4841 245.5882 61.88 0.1873 1921 +1923 4 221.0059 245.0402 60.1888 0.2364 1922 +1924 4 220.4705 244.8057 60.76 0.1271 1923 +1925 4 219.6491 244.1296 60.48 0.2288 1924 +1926 4 219.2224 243.7635 62.5162 0.2321 1925 +1927 4 218.6321 243.1309 63.4094 0.2669 1926 +1928 4 218.3736 242.3232 64.4028 0.3264 1927 +1929 4 217.5625 241.7592 64.3283 0.309 1928 +1930 4 216.8155 240.9344 64.12 0.3726 1929 +1931 4 216.0696 240.0947 64.3737 0.5053 1930 +1932 4 215.4655 239.3626 65.2288 0.5131 1931 +1933 4 215.0182 238.5366 66.0988 0.4272 1932 +1934 4 214.1248 237.9451 66.5414 0.3382 1933 +1935 4 213.3743 237.1054 66.6534 0.3432 1934 +1936 4 213.4693 236.236 66.9186 0.2671 1935 +1937 4 212.6204 235.6663 65.8 0.3358 1936 +1938 4 212.4374 234.7751 66.642 0.1922 1937 +1939 4 211.7498 234.3141 67.0466 0.3437 1938 +1940 4 211.2522 233.6048 67.7359 0.482 1939 +1941 4 210.5726 232.78 67.7757 0.3434 1940 +1942 4 209.9023 232.041 67.1216 0.3281 1941 +1943 4 209.4515 231.1921 66.9393 0.3765 1942 +1944 4 208.9951 230.3776 67.6855 0.353 1943 +1945 4 208.4814 229.69 68.88 0.4491 1944 +1946 4 207.9792 228.7222 68.698 0.2569 1945 +1947 4 207.5113 228.228 69.953 0.3875 1946 +1948 4 207.0137 227.4913 70.5186 0.2924 1947 +1949 4 206.3925 226.6939 70.74 0.4721 1948 +1950 4 205.5768 225.7146 68.9307 0.2469 1949 +1951 4 205.0071 225.0488 69.7799 0.3204 1950 +1952 4 204.355 224.2858 69.19 0.2288 1951 +1953 4 203.8883 223.7607 70.6776 0.3775 1952 +1954 4 203.6961 223.0514 70.6726 0.3268 1953 +1955 4 202.9788 222.6521 71.4692 0.472 1954 +1956 4 202.6138 221.6969 71.6864 0.3536 1955 +1957 4 201.9743 221.0116 72.142 0.3725 1956 +1958 4 201.36 220.1559 71.2379 0.2436 1957 +1959 4 201.1838 219.9088 72.266 0.1144 1958 +1960 4 200.812 219.171 73.67 0.1657 1959 +1961 4 200.2892 218.226 74.403 0.1977 1960 +1962 4 199.8465 217.2227 73.7769 0.2776 1961 +1963 4 199.5228 216.3704 72.6037 0.2765 1962 +1964 4 198.8009 215.5696 72.4802 0.394 1963 +1965 4 198.2403 214.6624 72.0619 0.2288 1964 +1966 4 198.0161 214.0893 74.1482 0.2818 1965 +1967 4 197.5082 213.3343 75.0126 0.4173 1966 +1968 4 196.9167 212.538 74.6911 0.2423 1967 +1969 4 196.5472 211.7372 75.3514 0.3432 1968 +1970 4 195.9706 211.0211 75.7884 0.4558 1969 +1971 4 195.6549 210.1459 75.7711 0.3156 1970 +1972 4 194.9628 209.7684 74.2092 0.4919 1971 +1973 4 194.5944 208.8052 75.0795 0.395 1972 +1974 4 194.2237 207.9346 75.8545 0.4263 1973 +1975 4 192.9001 206.7242 77.1 0.4235 1974 +1976 4 192.4151 206.031 77.5869 0.3298 1975 +1977 4 191.8946 205.3903 77.3111 0.2567 1976 +1978 4 191.5708 204.6536 77.28 0.2584 1977 +1979 4 190.8604 203.9763 77.9212 0.5339 1978 +1980 4 190.7689 203.4467 79.8238 0.3861 1979 +1981 4 190.2472 202.7317 80.0873 0.4454 1980 +1982 4 190.0276 201.7055 80.2984 0.3557 1981 +1983 4 189.2473 201.1518 80.0103 0.3756 1982 +1984 4 189.0071 200.3945 78.5932 0.3821 1983 +1985 4 188.7646 199.9083 80.1206 0.2245 1984 +1986 4 188.2612 199.2287 79.6816 0.3159 1985 +1987 4 187.3437 198.7471 79.002 0.4299 1986 +1988 4 186.6425 197.9566 79.469 0.3586 1987 +1989 4 185.9526 197.4601 80.3799 0.4454 1988 +1990 4 185.5728 197.1981 82.04 0.4195 1989 +1991 4 185.1312 196.1903 82.2343 0.3686 1990 +1992 4 184.3464 195.4181 82.7114 0.3313 1991 +1993 4 183.6074 194.623 83.4165 0.3811 1992 +1994 4 183.3294 193.5476 83.72 0.4602 1993 +1995 4 182.7197 192.732 83.5853 0.3615 1994 +1996 4 182.1214 191.8294 83.8956 0.353 1995 +1997 4 181.4773 191.1384 82.88 0.3813 1996 +1998 4 180.9716 190.2094 82.9116 0.3038 1997 +1999 4 180.315 189.4178 82.88 0.3704 1998 +2000 4 179.608 189.0014 83.54 0.2319 1999 +2001 4 179.3998 188.1811 84.5513 0.3051 2000 +2002 4 178.9124 187.33 84.6866 0.3691 2001 +2003 4 178.0201 186.758 84.9971 0.3392 2002 +2004 4 177.5728 185.8245 84.84 0.2953 2003 +2005 4 177.0923 184.8773 84.0062 0.4058 2004 +2006 4 176.6096 184.0833 84.0118 0.321 2005 +2007 4 175.8397 183.4953 84.5995 0.4174 2006 +2008 4 175.4873 182.9439 85.8561 0.3348 2007 +2009 4 174.9336 182.158 86.6746 0.2415 2008 +2010 4 174.6774 181.324 85.9636 0.4307 2009 +2011 4 174.3399 180.5953 87.08 0.3747 2010 +2012 4 173.6386 179.934 86.8935 0.4109 2011 +2013 4 173.3812 178.9754 86.8045 0.3424 2012 +2014 4 173.0769 178.3324 87.3152 0.2388 2013 +2015 4 172.8287 177.423 86.133 0.3051 2014 +2016 4 172.0576 176.6977 86.5354 0.4068 2015 +2017 4 171.6938 176.128 88.2837 0.3386 2016 +2018 4 171.1985 175.2608 89.0798 0.2352 2017 +2019 4 170.6619 174.6831 89.7005 0.3368 2018 +2020 4 170.1563 173.745 88.7788 0.2916 2019 +2021 4 169.5076 173.054 89.32 0.3295 2020 +2022 4 169.0729 172.3802 88.2059 0.3099 2021 +2023 4 168.3373 171.695 88.4366 0.1781 2022 +2024 4 167.5834 171.2614 89.6221 0.3887 2023 +2025 4 167.0675 170.5761 90.72 0.3487 2024 +2026 4 166.6922 169.5774 90.9504 0.2736 2025 +2027 4 166.0104 168.6805 90.8214 0.3615 2026 +2028 4 165.5334 167.8648 89.4449 0.4909 2027 +2029 4 164.776 167.2093 89.5843 0.4261 2028 +2030 4 164.2018 166.2575 89.6132 0.3665 2029 +2031 4 163.8826 165.6604 91.0482 0.337 2030 +2032 4 163.7007 164.641 90.5915 0.3375 2031 +2033 4 163.3964 163.616 91.0115 0.2341 2032 +2034 4 163.3312 162.7351 91.56 0.2229 2033 +2035 4 162.8347 161.9069 91.9881 0.3491 2034 +2036 4 162.0785 161.137 92.694 0.2981 2035 +2037 4 161.1759 160.5066 93.1837 0.2377 2036 +2038 4 160.7595 159.5045 93.2467 0.2034 2037 +2039 4 160.2561 158.5721 93.7045 0.2094 2038 +2040 4 159.7493 157.9235 93.0404 0.2898 2039 +2041 4 159.461 156.9625 93.5558 0.2677 2040 +2042 4 159.135 156.029 94.5098 0.2206 2041 +2043 4 158.6042 155.1664 95.1686 0.1738 2042 +2044 4 158.2884 154.0888 94.92 0.1192 2043 +2045 4 157.753 153.2171 95.2812 0.3227 2044 +2046 4 157.4236 152.1291 95.2 0.3284 2045 +2047 4 157.0575 151.0549 95.4198 0.2915 2046 +2048 4 156.6616 150.198 96.8103 0.2605 2047 +2049 4 156.2304 149.3995 97.7693 0.2601 2048 +2050 4 155.7579 148.5976 97.071 0.3911 2049 +2051 4 155.3323 147.7636 96.9422 0.3122 2050 +2052 4 154.8976 146.9548 98.4326 0.1401 2051 +2053 4 154.3245 146.0613 98.84 0.1144 2052 +2054 4 153.6049 145.3143 98.8308 0.1182 2053 +2055 4 152.8155 144.5398 98.4931 0.1898 2054 +2056 4 152.2458 143.5926 98.7792 0.1846 2055 +2057 4 151.5537 142.8616 99.12 0.3122 2056 +2058 4 150.7563 142.2644 98.7109 0.2288 2057 +2059 4 149.9933 141.8331 99.6128 0.2669 2058 +2060 4 149.7004 140.9419 100.6138 0.3392 2059 +2061 4 149.3698 139.9329 101.39 0.2084 2060 +2062 4 149.1593 138.8553 102.0124 0.124 2061 +2063 4 148.5187 137.9264 102.2 0.1144 2062 +2064 4 147.6549 137.3143 102.4666 0.1144 2063 +2065 4 146.9937 136.4243 102.48 0.1336 2064 +2066 4 146.4755 135.4599 102.7869 0.1725 2065 +2067 4 146.2146 134.4349 103.1414 0.1635 2066 +2068 4 145.7559 133.5448 103.9004 0.1144 2067 +2069 4 145.3418 132.5198 104.16 0.1144 2068 +2070 4 144.4769 131.8952 104.2272 0.1225 2069 +2071 4 143.7081 131.1173 104.4291 0.2116 2070 +2072 4 143.5617 130.5693 106.3866 0.167 2071 +2073 4 143.1201 129.7182 106.6985 0.1939 2072 +2074 4 142.7815 128.7927 106.2384 0.2288 2073 +2075 4 142.4257 127.8614 106.7878 0.1907 2074 +2076 4 142.0779 127.0458 107.8 0.1714 2075 +2077 4 141.7038 125.9922 107.7997 0.1907 2076 +2078 4 141.0678 125.109 107.8246 0.1325 2077 +2079 4 140.2292 124.5278 108.5468 0.1144 2078 +2080 4 139.4547 123.7064 108.64 0.1144 2079 +2081 4 138.8587 122.9651 109.482 0.1144 2080 +2082 4 138.5338 122.0202 109.9134 0.1312 2081 +2083 4 137.9515 121.089 110.2469 0.1191 2082 +2084 4 137.1919 120.3133 111.0547 0.1514 2083 +2085 4 136.7034 119.3112 111.517 0.2034 2084 +2086 4 136.16 118.3376 112.0 0.162 2085 +2087 4 136.033 117.3241 112.572 0.116 2086 +2088 4 136.2298 116.2876 113.3457 0.1341 2087 +2089 4 136.0857 115.1791 113.68 0.1487 2088 +2090 4 135.7676 114.0822 113.68 0.2288 2089 +2091 4 135.3981 113.5568 113.96 0.3382 2090 +2092 4 134.8776 112.7799 114.7471 0.3216 2091 +2093 4 134.8421 111.7206 115.0932 0.265 2092 +2094 4 135.4416 110.8978 115.4829 0.2255 2093 +2095 4 135.6933 110.0838 114.0 0.2614 2094 +2096 4 135.6784 109.1147 114.408 0.2728 2095 +2097 4 135.9644 108.3403 116.1345 0.2731 2096 +2098 4 135.7734 107.3832 116.247 0.2754 2097 +2099 4 135.8157 106.3778 116.713 0.1801 2098 +2100 4 135.7562 105.2764 117.2979 0.2305 2099 +2101 4 135.4313 104.1866 117.4362 0.2765 2100 +2102 4 135.1762 103.141 118.1471 0.2258 2101 +2103 4 134.6808 102.7232 116.5688 0.1973 2102 +2104 4 134.6305 101.8254 115.64 0.2256 2103 +2105 4 134.6488 100.7918 116.0944 0.1144 2104 +2106 4 134.6911 100.0884 117.6512 0.148 2105 +2107 4 134.7815 99.3526 118.72 0.3813 2106 +2108 4 135.2116 98.4157 118.2686 0.3241 2107 +2109 4 135.2437 97.7976 116.3408 0.2519 2108 +2110 4 135.54 96.8788 117.32 0.1947 2109 +2111 4 135.6384 96.0939 116.9518 0.2655 2110 +2112 4 135.5686 95.3231 116.0323 0.2111 2111 +2113 4 135.961 94.9299 114.4307 0.2341 2112 +2114 4 136.247 93.8935 113.96 0.2448 2113 +2115 4 136.3568 92.8218 114.1753 0.2625 2114 +2116 4 136.4529 91.8987 115.36 0.1849 2115 +2117 4 137.0512 91.0443 115.5636 0.2385 2116 +2118 4 136.9402 90.948 118.258 0.1332 2117 +2119 4 136.9368 90.9226 121.0527 0.1144 2118 +2120 4 137.0512 90.5832 123.489 0.1144 2119 +2121 4 137.0638 89.7208 125.1228 0.1144 2120 +2122 4 137.0215 89.0255 127.1393 0.1349 2121 +2123 4 137.0592 88.0054 127.9911 0.2262 2122 +2124 4 137.1805 86.96 128.7784 0.1232 2123 +2125 4 137.8566 86.1886 129.0635 0.1719 2124 +2126 4 138.0808 85.5975 129.8321 0.2235 2125 +2127 4 138.6082 84.7646 130.188 0.1144 2126 +2128 4 139.2008 83.8653 130.76 0.2266 2127 +2129 4 139.465 83.1791 129.3804 0.1773 2128 +2130 4 140.2121 82.5405 129.2609 0.1562 2129 +2131 4 140.8264 81.6683 129.08 0.1182 2130 +2132 4 141.6215 80.9534 129.0187 0.1144 2131 +2133 4 142.5664 80.3992 128.5889 0.1144 2132 +2134 4 143.1178 79.4848 128.3582 0.1144 2133 +2135 4 143.2288 78.4465 128.4875 0.1144 2134 +2136 4 143.0149 77.6787 129.3454 0.1675 2135 +2137 4 143.1522 77.233 131.0431 0.2472 2136 +2138 4 143.4702 76.4309 132.16 0.2478 2137 +2139 4 143.8706 75.5771 132.6601 0.2069 2138 +2140 4 143.7962 74.8564 134.1166 0.1254 2139 +2141 4 143.3032 74.0542 135.2425 0.1144 2140 +2142 4 143.0801 73.2361 136.2155 0.1468 2141 +2143 4 143.2174 72.1136 136.08 0.2382 2142 +2144 4 143.4576 71.3856 136.64 0.1398 2143 +2145 4 135.3993 113.6063 114.2098 0.3051 2090 +2146 4 134.6705 113.0582 113.1259 0.2772 2145 +2147 4 134.2839 112.9949 114.6107 0.3276 2146 +2148 4 133.7771 112.7324 116.48 0.2415 2147 +2149 4 133.0724 112.0937 117.7081 0.2775 2148 +2150 4 132.9751 111.1348 118.6234 0.2504 2149 +2151 4 132.3414 110.3951 118.454 0.1563 2150 +2152 4 131.56 110.0009 119.576 0.273 2151 +2153 4 131.2111 109.3296 121.2224 0.1453 2152 +2154 4 130.6368 108.7944 122.2931 0.2055 2153 +2155 4 129.9607 108.2954 121.2926 0.265 2154 +2156 4 129.5225 107.9852 122.703 0.1314 2155 +2157 4 129.725 107.3733 124.2497 0.1478 2156 +2158 4 129.2892 106.8544 123.7054 0.2288 2157 +2159 4 128.5948 106.3221 124.88 0.2314 2158 +2160 4 128.3431 105.2986 125.5346 0.1652 2159 +2161 4 127.4553 104.8079 126.28 0.1144 2160 +2162 4 126.8971 104.0031 127.073 0.2605 2161 +2163 4 125.9418 103.4019 127.12 0.2765 2162 +2164 4 125.1742 102.8517 127.9116 0.1907 2163 +2165 4 124.4523 102.6348 129.64 0.1271 2164 +2166 4 123.5692 101.9666 129.92 0.1333 2165 +2167 4 122.8233 101.3039 130.7348 0.1265 2166 +2168 4 121.8028 100.8097 130.76 0.131 2167 +2169 4 120.9437 100.4054 131.224 0.2008 2168 +2170 4 120.0754 99.7797 130.6836 0.1849 2169 +2171 4 119.4061 99.3095 129.1987 0.1302 2170 +2172 4 118.515 99.143 128.6659 0.1144 2171 +2173 4 117.6696 98.5148 128.24 0.1144 2172 +2174 4 116.5496 98.384 128.4363 0.1144 2173 +2175 4 115.409 98.384 128.52 0.1194 2174 +2176 4 114.5167 98.384 129.7778 0.1619 2175 +2177 4 113.8957 98.4984 132.0063 0.2288 2176 +2178 4 113.0108 98.9001 132.0852 0.2868 2177 +2179 4 112.0936 99.2735 131.3301 0.2418 2178 +2180 4 111.1802 99.3057 131.068 0.2135 2179 +2181 4 110.123 99.362 131.0397 0.2287 2180 +2182 4 109.268 99.4342 132.02 0.2431 2181 +2183 4 108.3749 99.0196 131.6311 0.3178 2182 +2184 4 107.9407 98.8416 132.7418 0.3199 2183 +2185 4 107.5813 98.384 132.7782 0.1477 2184 +2186 4 107.269 98.023 131.2394 0.2898 2185 +2187 4 106.4616 97.3663 131.6078 0.1263 2186 +2188 4 105.8261 97.391 133.2612 0.1144 2187 +2189 4 105.6227 96.6324 133.56 0.1271 2188 +2190 4 105.4783 95.6784 132.9051 0.1907 2189 +2191 4 104.7904 95.5017 131.4712 0.1504 2190 +2192 4 104.719 94.465 130.975 0.2004 2191 +2193 4 104.7546 93.4246 131.0512 0.1788 2192 +2194 4 104.9176 92.3282 131.0501 0.1566 2193 +2195 4 105.1714 91.5068 129.8564 0.1522 2194 +2196 4 104.9646 90.7354 129.5868 0.2288 2195 +2197 4 105.1305 90.376 131.8929 0.1715 2196 +2198 4 105.0192 89.9533 133.387 0.313 2197 +2199 4 104.676 89.1177 132.6399 0.1953 2198 +2200 4 104.4472 88.307 133.5298 0.2743 2199 +2201 4 104.3328 87.3038 132.4621 0.2692 2200 +2202 4 104.0619 86.414 130.9991 0.1921 2201 +2203 4 103.2351 85.8715 130.3224 0.2015 2202 +2204 4 103.0362 85.1773 128.8319 0.1907 2203 +2205 4 103.293 84.8392 127.1651 0.155 2204 +2206 4 103.6284 83.8574 126.84 0.1981 2205 +2207 4 102.9944 83.0582 127.132 0.1682 2206 +2208 4 102.325 82.3305 126.9036 0.3387 2207 +2209 4 102.1894 81.4099 126.5561 0.1382 2208 +2210 4 101.5258 80.9671 124.6666 0.2407 2209 +2211 4 101.2654 80.3004 123.9762 0.1228 2210 +2212 4 101.538 79.6142 124.1198 0.2542 2211 +2213 4 101.5872 78.8216 124.04 0.2542 2212 +2214 4 107.6236 98.956 132.314 0.249 2183 +2215 4 107.0173 99.6743 131.8738 0.2094 2214 +2216 4 106.4612 100.224 130.5581 0.1718 2215 +2217 4 105.7241 100.6961 131.5591 0.1652 2216 +2218 4 105.3258 101.2052 133.0 0.2547 2217 +2219 4 104.5105 101.914 132.7306 0.2672 2218 +2220 4 103.7482 102.3552 133.7563 0.1605 2219 +2221 4 103.0297 102.8874 134.3734 0.1922 2220 +2222 4 102.4516 103.6337 134.0268 0.2288 2221 +2223 4 101.816 103.9133 134.2132 0.2288 2222 +2224 4 101.6215 104.0517 134.9334 0.1416 2222 +2225 4 100.8323 104.4483 134.7819 0.2519 2224 +2226 4 100.2006 104.9818 135.4564 0.1777 2225 +2227 4 99.5304 105.5897 134.6374 0.1144 2226 +2228 4 99.1649 106.396 135.5973 0.142 2227 +2229 4 98.4944 106.9781 136.8906 0.1144 2228 +2230 4 97.8302 107.3853 138.1134 0.2188 2229 +2231 4 97.2462 107.7832 139.806 0.1657 2230 +2232 4 97.0504 108.4184 139.7536 0.2012 2231 +2233 4 96.6121 109.1376 140.4455 0.2718 2232 +2234 4 96.2954 109.7806 141.9558 0.2051 2233 +2235 4 95.3715 109.824 143.2668 0.2034 2234 +2236 4 94.4802 110.2816 143.5031 0.2134 2235 +2237 4 93.6863 110.4196 144.8868 0.1568 2236 +2238 4 93.1176 111.073 144.9384 0.1398 2237 +2239 4 92.3406 110.968 143.7419 0.2631 2238 +2240 4 91.5187 110.8489 145.4379 0.1954 2239 +2241 4 90.5336 110.5398 146.5738 0.1652 2240 +2242 4 89.9027 110.7393 148.6492 0.178 2241 +2243 4 88.9348 111.0824 147.9848 0.2539 2242 +2244 4 87.9145 110.9666 146.886 0.1586 2243 +2245 4 86.8543 111.2734 146.7357 0.1652 2244 +2246 4 85.9644 111.8899 147.499 0.1652 2245 +2247 4 85.1134 112.2264 148.4115 0.1905 2246 +2248 4 84.7254 112.2592 150.6086 0.1499 2247 +2249 4 83.9685 112.6759 151.5816 0.2261 2248 +2250 4 83.2832 112.4552 149.8 0.1525 2249 +2251 4 223.8064 247.0079 61.161 0.2799 1920 +2252 4 224.224 247.9048 59.92 0.2796 2251 +2253 4 248.3876 280.598 49.28 0.3178 1869 +2254 4 248.5306 279.4792 50.3552 0.2932 2253 +2255 4 249.3497 278.9861 51.3752 0.2613 2254 +2256 4 249.1907 278.0355 50.5926 0.1907 2255 +2257 4 249.5064 277.1512 51.711 0.242 2256 +2258 4 249.527 276.2806 52.5893 0.2756 2257 +2259 4 250.3232 275.6662 51.8263 0.138 2258 +2260 4 250.5463 274.6538 52.3508 0.2656 2259 +2261 4 250.9032 273.8999 52.4465 0.1332 2260 +2262 4 250.8792 272.812 52.92 0.1707 2261 +2263 4 251.227 271.8853 53.2734 0.2374 2262 +2264 4 251.6583 271.239 53.7068 0.2372 2263 +2265 4 251.7944 270.4576 54.2497 0.2288 2264 +2266 4 251.7944 269.5447 55.0642 0.2288 2265 +2267 4 251.68 268.8469 54.962 0.3373 2266 +2268 4 252.1902 268.165 55.5307 0.115 2267 +2269 4 252.7222 267.3551 54.7929 0.2075 2268 +2270 4 253.1478 266.5394 56.1554 0.2224 2269 +2271 4 253.7323 265.6059 56.203 0.2669 2270 +2272 4 253.396 264.725 56.5452 0.2542 2271 +2273 4 253.1066 263.7778 56.3046 0.2288 2272 +2274 4 253.6751 262.8489 56.3634 0.2504 2273 +2275 4 254.3112 262.2197 56.6989 0.3893 2274 +2276 4 254.6544 261.404 58.002 0.2911 2275 +2277 4 254.5812 260.3504 57.6265 0.1184 2276 +2278 4 253.9188 259.9088 56.7602 0.1326 2277 +2279 4 253.7312 259.3448 57.5523 0.3715 2278 +2280 4 253.865 258.5577 57.1096 0.178 2279 +2281 4 254.8043 258.417 57.148 0.323 2280 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243.4672 305.6127 46.7082 0.2855 2369 +2371 4 243.5542 306.4707 47.4116 0.3405 2370 +2372 4 243.577 307.3951 48.5206 0.3191 2371 +2373 4 243.9992 308.1318 48.7589 0.2129 2372 +2374 4 243.5576 308.8972 48.5593 0.1179 2373 +2375 4 243.5576 309.6362 46.7286 0.2052 2374 +2376 4 243.2144 310.4267 45.4168 0.1329 2375 +2377 4 243.434 311.3648 45.8693 0.2063 2376 +2378 4 243.4066 312.0512 47.6 0.2262 2377 +2379 4 243.0108 313.0728 47.1414 0.1305 2378 +2380 4 242.5349 313.9571 46.5548 0.2086 2379 +2381 4 242.1676 315.0187 46.4145 0.2542 2380 +2382 4 241.8256 315.99 45.876 0.1723 2381 +2383 4 241.1884 316.8388 45.64 0.2481 2382 +2384 4 240.1233 317.1145 45.6218 0.1734 2383 +2385 4 239.3202 317.3811 45.1825 0.1563 2384 +2386 4 238.349 317.6934 45.1732 0.1953 2385 +2387 4 237.7953 318.612 45.5972 0.1612 2386 +2388 4 237.2816 319.5112 44.8636 0.1991 2387 +2389 4 236.4648 320.185 44.8146 0.1443 2388 +2390 4 235.7201 320.6666 44.24 0.1144 2389 +2391 4 235.1606 321.6642 44.24 0.1144 2390 +2392 4 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2435 +2437 4 298.9192 341.6213 29.1942 0.2849 2436 +2438 4 299.9053 342.0423 29.986 0.2629 2437 +2439 4 300.6821 341.3993 29.7195 0.3051 2438 +2440 4 301.6614 341.2186 29.3048 0.1683 2439 +2441 4 302.6864 340.912 29.68 0.3548 2440 +2442 4 303.303 340.2199 28.4917 0.341 2441 +2443 4 304.2662 339.8172 27.7732 0.2851 2442 +2444 4 305.067 339.3642 28.3984 0.171 2443 +2445 4 305.6002 338.5794 28.94 0.1444 2444 +2446 4 306.1344 337.9147 29.6363 0.2804 2445 +2447 4 306.3655 337.8003 27.72 0.2755 2446 +2448 4 307.2006 337.4262 27.7794 0.3077 2447 +2449 4 307.8435 336.8886 28.7468 0.2211 2448 +2450 4 308.6501 336.241 28.2559 0.3305 2449 +2451 4 309.4074 335.9928 27.9028 0.2812 2450 +2452 4 310.1933 335.4505 27.5775 0.3529 2451 +2453 4 310.834 334.668 27.1043 0.37 2452 +2454 4 311.7549 334.1075 26.6213 0.2924 2453 +2455 4 312.8096 333.8615 27.1384 0.309 2454 +2456 4 313.5578 333.1957 27.6273 0.3557 2455 +2457 4 314.0566 332.5448 26.9713 0.3299 2456 +2458 4 314.8929 332.0815 26.5826 0.2924 2457 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287.2344 327.9974 28.0 0.2619 2546 +2548 4 286.4061 327.43 28.5463 0.1818 2547 +2549 4 285.3788 327.1485 27.9919 0.2734 2548 +2550 4 284.8766 326.3946 26.9802 0.3022 2549 +2551 4 284.0049 325.8123 26.8688 0.2417 2550 +2552 4 283.4809 325.0962 26.9111 0.2415 2551 +2553 4 283.1148 324.2988 27.1603 0.1528 2552 +2554 4 282.2671 324.0929 26.476 0.117 2553 +2555 4 281.2982 323.752 26.6874 0.1669 2554 +2556 4 280.4196 323.8161 26.63 0.1611 2555 +2557 4 279.5787 323.3745 26.857 0.2408 2556 +2558 4 278.4976 323.0702 26.6916 0.2163 2557 +2559 4 277.6259 322.9489 25.76 0.2853 2558 +2560 4 276.9498 322.2396 26.3388 0.2186 2559 +2561 4 275.9774 322.0703 25.7673 0.2288 2560 +2562 4 274.981 321.9559 24.6616 0.3012 2561 +2563 4 274.2431 321.8312 25.9028 0.2474 2562 +2564 4 273.1449 321.9216 25.716 0.3025 2563 +2565 4 272.4242 322.5028 24.638 0.2892 2564 +2566 4 271.748 322.5508 22.7035 0.2638 2565 +2567 4 270.8477 322.6892 23.0023 0.2004 2566 +2568 4 269.857 322.862 22.4932 0.1907 2567 +2569 4 269.412 322.8162 22.7172 0.2383 2568 +2570 4 268.9544 322.4936 23.8 0.1398 2569 +2571 4 269.2713 322.9512 22.4319 0.24 2568 +2572 4 268.284 323.2006 22.0886 0.2415 2571 +2573 4 267.3356 323.1892 21.7022 0.2669 2572 +2574 4 266.3507 322.989 21.1994 0.2325 2573 +2575 4 265.8301 323.4969 22.8928 0.1907 2574 +2576 4 265.2982 323.9888 21.6639 0.2246 2575 +2577 4 265.1872 324.7564 21.0683 0.1221 2576 +2578 4 264.6083 325.3536 21.2783 0.2289 2577 +2579 4 263.8007 325.6396 21.0157 0.1779 2578 +2580 4 262.6956 325.5515 21.0356 0.3021 2579 +2581 4 261.7072 325.5927 21.4749 0.1282 2580 +2582 4 260.9281 325.7517 21.7305 0.2034 2581 +2583 4 260.0575 325.7746 21.0339 0.1907 2582 +2584 4 259.3448 325.905 21.3052 0.1456 2583 +2585 4 258.7797 326.2139 19.3486 0.1671 2584 +2586 4 257.7661 326.1498 19.1464 0.2288 2585 +2587 4 257.4 326.3821 20.4674 0.1144 2586 +2588 4 256.8108 325.7929 20.6774 0.2033 2587 +2589 4 256.1473 325.2403 19.0994 0.2658 2588 +2590 4 255.0914 325.1648 19.0725 0.2533 2589 +2591 4 254.7345 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367.2274 41.7816 0.3514 2612 +2614 4 295.4952 368.2708 42.1366 0.3822 2613 +2615 4 295.6096 369.385 42.5919 0.2795 2614 +2616 4 296.2331 370.0588 43.7928 0.185 2615 +2617 4 296.5317 371.0942 43.7464 0.1497 2616 +2618 4 297.3256 371.8 43.4 0.2288 2617 +2619 4 292.4053 364.936 41.7189 0.3181 2609 +2620 4 291.8802 365.0504 43.143 0.3432 2619 +2621 4 290.7728 365.0321 43.5554 0.286 2620 +2622 4 289.8976 364.7072 42.7706 0.2392 2621 +2623 4 289.1368 364.7358 42.8515 0.2048 2622 +2624 4 288.24 364.1386 43.5266 0.3156 2623 +2625 4 287.3602 363.4614 43.68 0.4449 2624 +2626 4 286.3112 363.5632 44.5917 0.3148 2625 +2627 4 285.3834 363.832 45.808 0.1718 2626 +2628 4 284.3172 363.6776 45.6646 0.2382 2627 +2629 4 283.4443 363.0793 45.7114 0.272 2628 +2630 4 282.5383 362.9866 46.6374 0.194 2629 +2631 4 281.6173 362.6926 46.7496 0.1398 2630 +2632 4 280.8188 363.403 46.6312 0.3362 2631 +2633 4 279.7824 363.5174 47.031 0.3419 2632 +2634 4 279.1463 362.9912 48.0472 0.3432 2633 +2635 4 278.3947 362.4501 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15.1718 0.3009 3761 +3763 3 209.0077 332.8434 14.6695 0.2384 3762 +3764 3 207.9712 332.5928 15.12 0.4115 3763 +3765 3 206.9084 332.2256 14.7008 0.302 3764 +3766 3 205.9177 331.7657 14.6818 0.2727 3765 +3767 3 204.9453 332.157 14.6793 0.2608 3766 +3768 3 204.3607 332.6935 13.1911 0.3037 3767 +3769 3 203.4043 332.7175 12.3166 0.3312 3768 +3770 3 202.5727 332.904 12.0851 0.1533 3769 +3771 3 201.5362 332.8411 12.7812 0.2616 3770 +3772 3 200.6748 332.5608 13.1897 0.1541 3771 +3773 3 199.7824 332.801 14.1159 0.142 3772 +3774 3 198.7483 333.0424 13.8667 0.3057 3773 +3775 3 198.5481 333.484 12.868 0.3686 3774 +3776 3 198.1717 333.7345 11.3226 0.3026 3775 +3777 3 197.7427 334.5102 12.3385 0.2186 3776 +3778 3 196.7028 334.8694 12.9396 0.2089 3777 +3779 3 195.7636 334.8191 12.9847 0.1992 3778 +3780 3 195.5027 334.8431 11.3277 0.1794 3779 +3781 3 194.8655 334.2619 10.9175 0.1261 3780 +3782 3 194.1711 333.5263 11.3341 0.2603 3781 +3783 3 194.1368 332.904 12.04 0.1144 3782 diff --git a/docs/tutorial/04_opt_advanced_inputs/components/stimulations/xstim_coords.csv b/docs/tutorial/04_opt_advanced_inputs/components/stimulations/xstim_coords.csv new file mode 100644 index 00000000..d2b1f2f6 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/stimulations/xstim_coords.csv @@ -0,0 +1,2 @@ +ip pos_x pos_y pos_z rotation_x rotation_y rotation_z +0 6.1803398874989481 0.0 19.021130325903069 0. 0. 0. diff --git a/docs/tutorial/04_opt_advanced_inputs/components/synaptic_models/AMPA_ExcToExc.json b/docs/tutorial/04_opt_advanced_inputs/components/synaptic_models/AMPA_ExcToExc.json new file mode 100644 index 00000000..c7585403 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/components/synaptic_models/AMPA_ExcToExc.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 1.0, + "tau2": 3.0, + "erev": 0.0 +} diff --git a/docs/tutorial/04_opt_advanced_inputs/config.cell_types.json b/docs/tutorial/04_opt_advanced_inputs/config.cell_types.json new file mode 100644 index 00000000..b91161c6 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.cell_types.json @@ -0,0 +1,67 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_cell_types", + "$INPUT_DIR": "$BASE_DIR/ephys_inputs", + "$NETWORK_DIR": "$BASE_DIR/network_cell_types", + "$MODEL_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_nwb": { + "module": "IClamp", + "input_type": "allen", + "node_set": "all", + "file": "${INPUT_DIR}/488683423_ephys.nwb", + "sweep_id": "35" + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": [0], + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "$MODEL_DIR/morphologies", + "mechanisms_dir":"$MODEL_DIR/mechanisms", + "biophysical_neuron_models_dir": "$MODEL_DIR/biophysical_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/bio_488683425_fullstub_nodes.h5", + "node_types_file": "$NETWORK_DIR/bio_488683425_fullstub_node_types.csv" + } + ] + } + } \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.csv_series.json b/docs/tutorial/04_opt_advanced_inputs/config.csv_series.json new file mode 100644 index 00000000..42af2be5 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.csv_series.json @@ -0,0 +1,73 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_csv_series", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_current_clamp", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_series": { + "input_type": "csv", + "module": "IClamp", + "node_set": { + "population": "net", + "model_type": "biophysical" + }, + "file": "$INPUT_DIR/iclamp_inputs_series.csv", + "section_name": "soma" + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": {"population": "net"}, + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "components/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "components/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + } + ] + } + } + \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.csv_wave.json b/docs/tutorial/04_opt_advanced_inputs/config.csv_wave.json new file mode 100644 index 00000000..a64fac7e --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.csv_wave.json @@ -0,0 +1,73 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_csv_wave", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_current_clamp", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_series": { + "input_type": "csv", + "module": "IClamp", + "node_set": { + "population": "net", + "model_type": "biophysical" + }, + "file": "$INPUT_DIR/iclamp_damped_wave.csv", + "section_name": "soma" + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": {"population": "net"}, + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "components/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "components/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + } + ] + } + } + \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.current_clamp.json b/docs/tutorial/04_opt_advanced_inputs/config.current_clamp.json new file mode 100644 index 00000000..3db26edb --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.current_clamp.json @@ -0,0 +1,77 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_current_clamp", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_current_clamp", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_1": { + "input_type": "current_clamp", + "module": "IClamp", + "node_set": { + "population": "net", + "node_id": [0] + }, + "amp": 0.1000, + "delay": 500.0, + "duration": 1000.0, + "section_name": "soma", + "section_index": 0, + "section_dist": 0.5 + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": {"population": "net"}, + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "components/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "components/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + } + ] + } + } + \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.current_clamp_allcells.json b/docs/tutorial/04_opt_advanced_inputs/config.current_clamp_allcells.json new file mode 100644 index 00000000..fd86df58 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.current_clamp_allcells.json @@ -0,0 +1,77 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_current_clamp", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_current_clamp", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_1": { + "input_type": "current_clamp", + "module": "IClamp", + "node_set": { + "population": "net", + "model_type": "biophysical" + }, + "amp": 0.1000, + "delay": 500.0, + "duration": 1000.0, + "section_name": "soma", + "section_index": 0, + "section_dist": 0.5 + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": {"population": "net"}, + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "components/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "components/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + } + ] + } + } + \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.current_clamp_apic.json b/docs/tutorial/04_opt_advanced_inputs/config.current_clamp_apic.json new file mode 100644 index 00000000..8bd02be5 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.current_clamp_apic.json @@ -0,0 +1,76 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_current_clamp", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_current_clamp", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_apic": { + "input_type": "current_clamp", + "module": "IClamp", + "node_set": { + "population": "net", + "node_id": [0] + }, + "amp": 0.1000, + "delay": 500.0, + "duration": 1000.0, + "section_name": ["dend", "apic"], + "section_index": [500.0, 1.0e20] + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": {"population": "net"}, + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "components/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "components/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + } + ] + } + } + \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.voltage_clamp.json b/docs/tutorial/04_opt_advanced_inputs/config.voltage_clamp.json new file mode 100644 index 00000000..951cad79 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.voltage_clamp.json @@ -0,0 +1,85 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_voltage_clamp", + "$INPUT_DIR": "$BASE_DIR/inputs", + "$NETWORK_DIR": "$BASE_DIR/network_voltage_clamp", + "$COMPONENT_DIR": "$BASE_DIR/components" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "se_clamp_1": { + "input_type": "clamp", + "module": "SEClamp", + "node_set": { + "population": "net", + "node_ids": [0] + }, + "amp": 20.0, + "duration": 1000.0, + "delay": 1000.0, + "section_name": "soma", + "section_index": 0, + "section_dist": 0.5 + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": { + "population": "net" + }, + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "components/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "components/biophysical_neuron_templates", + "point_neuron_models_dir": "$COMPONENT_DIR/point_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/net_nodes.h5", + "node_types_file": "$NETWORK_DIR/net_node_types.csv" + } + ], + "edges": [ + { + "edges_file": "$NETWORK_DIR/net_net_edges.h5", + "edge_types_file": "$NETWORK_DIR/net_net_edge_types.csv" + } + ] + } + } + \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/config.xstim_custom.json b/docs/tutorial/04_opt_advanced_inputs/config.xstim_custom.json new file mode 100644 index 00000000..1b461ab0 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.xstim_custom.json @@ -0,0 +1,80 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_xstim", + "$COMPONENT_DIR": "$BASE_DIR/components", + "$STIM_DIR": "$BASE_DIR/components/stimulations", + "$NETWORK_DIR": "$BASE_DIR/network_xstim" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 10000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "Extracellular_Stim": { + "module": "xstim", + "input_type": "lfp", + "node_set": { + "model_type": "biophysical" + }, + "positions_file": "$STIM_DIR/xstim_coords.csv", + "resistance": 300.0, + "waveform": "inputs/ramping_xstim.csv" + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time" + }, + + "node_sets": { + "biophys_cells": { + "model_type": "biophysical" + } + }, + + "reports": { + + "membrane_potential": { + "cells": "biophys_cells", + "variable_name": "v", + "module": "membrane_report", + "file_name": "v_report.h5", + "sections": "soma", + "enabled": true + } + + }, + + "components": { + "morphologies_dir": "$COMPONENT_DIR/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "$COMPONENT_DIR/biophysical_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/bio_nodes.h5", + "node_types_file": "$NETWORK_DIR/bio_node_types.csv" + } + ] + } +} diff --git a/docs/tutorial/04_opt_advanced_inputs/config.xstim_dc.json b/docs/tutorial/04_opt_advanced_inputs/config.xstim_dc.json new file mode 100644 index 00000000..5a1e0b2c --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.xstim_dc.json @@ -0,0 +1,83 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_xstim", + "$COMPONENT_DIR": "$BASE_DIR/components", + "$STIM_DIR": "$BASE_DIR/components/stimulations", + "$NETWORK_DIR": "$BASE_DIR/network_xstim" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 10000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "Extracellular_Stim": { + "input_type": "lfp", + "node_set": "all", + "module": "xstim", + "positions_file": "$STIM_DIR/xstim_coords.csv", + "resistance": 300.0, + "waveform": { + "shape": "dc", + "del": 1000.0, + "amp": 0.400, + "dur": 1000.0 + } + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time" + }, + + "node_sets": { + "biophys_cells": { + "model_type": "biophysical" + } + }, + + "reports": { + + "membrane_potential": { + "cells": "biophys_cells", + "variable_name": "v", + "module": "membrane_report", + "file_name": "v_report.h5", + "sections": "soma", + "enabled": true + } + + }, + + "components": { + "morphologies_dir": "$COMPONENT_DIR/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "$COMPONENT_DIR/biophysical_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/bio_nodes.h5", + "node_types_file": "$NETWORK_DIR/bio_node_types.csv" + } + ] + } +} diff --git a/docs/tutorial/04_opt_advanced_inputs/config.xstim_sin.json b/docs/tutorial/04_opt_advanced_inputs/config.xstim_sin.json new file mode 100644 index 00000000..7dd785bf --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/config.xstim_sin.json @@ -0,0 +1,87 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_xstim", + "$COMPONENT_DIR": "$BASE_DIR/components", + "$STIM_DIR": "$BASE_DIR/components/stimulations", + "$NETWORK_DIR": "$BASE_DIR/network_xstim" + }, + + "run": { + "tstop": 4000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 10000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "Extracellular_Stim": { + "module": "xstim", + "input_type": "lfp", + "node_set": { + "model_type": "biophysical" + }, + "positions_file": "$STIM_DIR/xstim_coords.csv", + "resistance": 300.0, + "waveform": { + "shape": "sin", + "del": 1000.0, + "amp": 0.100, + "dur": 2000.0, + "freq": 8.0, + "phase": 0.0 + } + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time" + }, + + "node_sets": { + "biophys_cells": { + "model_type": "biophysical" + } + }, + + "reports": { + + "membrane_potential": { + "cells": "biophys_cells", + "variable_name": "v", + "module": "membrane_report", + "file_name": "v_report.h5", + "sections": "soma", + "enabled": true + } + + }, + + "components": { + "morphologies_dir": "$COMPONENT_DIR/morphologies", + "synaptic_models_dir": "$COMPONENT_DIR/synaptic_models", + "mechanisms_dir":"$COMPONENT_DIR/mechanisms", + "biophysical_neuron_models_dir": "$COMPONENT_DIR/biophysical_neuron_templates" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/bio_nodes.h5", + "node_types_file": "$NETWORK_DIR/bio_node_types.csv" + } + ] + } +} diff --git a/docs/tutorial/04_opt_advanced_inputs/create_ramping_stim.py b/docs/tutorial/04_opt_advanced_inputs/create_ramping_stim.py new file mode 100644 index 00000000..0f975d25 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/create_ramping_stim.py @@ -0,0 +1,16 @@ +import numpy as np +import pandas as pd +import matplotlib.pyplot as plt + + +time = np.linspace(0.0, 3000.0, 1000) +# amplitude = time*0.0003-.100 +amplitude = time*0.0002-.100 + + +pd.DataFrame({ + 'time': time, + 'amplitude': amplitude +}).to_csv('inputs/ramping_xstim.csv', sep=' ', index=False) +plt.plot(time, amplitude) +plt.show() \ No newline at end of file diff --git a/docs/tutorial/04_opt_advanced_inputs/inputs/iclamp_damped_wave.csv b/docs/tutorial/04_opt_advanced_inputs/inputs/iclamp_damped_wave.csv new file mode 100644 index 00000000..d78da59a --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/inputs/iclamp_damped_wave.csv @@ -0,0 +1,151 @@ +timestamps amps +0.0 0.0 +20.0 0.0183670915959403 +40.0 0.03536901825121116 +60.0 0.05046226716553377 +80.0 0.06319166233544364 +100.0 0.07320288483374399 +120.0 0.08025096045266605 +140.0 0.0842045747795784 +160.0 0.08504622778524194 +180.0 0.08286838563111748 +200.0 0.07786592178060531 +220.0 0.07032525835152915 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sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + run('config.current_clamp.json') + diff --git a/docs/tutorial/04_opt_advanced_inputs/run_bmtk.voltage_clamp.py b/docs/tutorial/04_opt_advanced_inputs/run_bmtk.voltage_clamp.py new file mode 100644 index 00000000..18f38f4b --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/run_bmtk.voltage_clamp.py @@ -0,0 +1,27 @@ +import os +import sys + +from bmtk.simulator import bionet +from bmtk.analyzer.compartment import plot_traces + + + +def run(config_path): + conf = bionet.Config.from_json(config_path, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + + plot_traces(config_file=config_path, report_name='membrane_potential', population='net', node_ids=[0, 1]) + # bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + run('config.current_clamp.json') + diff --git a/docs/tutorial/04_opt_advanced_inputs/run_bmtk.xstim.py b/docs/tutorial/04_opt_advanced_inputs/run_bmtk.xstim.py new file mode 100644 index 00000000..ad24fd86 --- /dev/null +++ b/docs/tutorial/04_opt_advanced_inputs/run_bmtk.xstim.py @@ -0,0 +1,23 @@ +"""Simulates an example network of 450 cell receiving two kinds of exernal input as defined in the configuration file""" +import sys + +from bmtk.simulator import bionet +from bmtk.analyzer.compartment import plot_traces + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + + plot_traces(config_file=config_file, report_name='membrane_potential', population='bio') + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + run(sys.argv[-1]) + else: + run('config.xstim.json') # Extracellular electrode stimulation diff --git a/docs/tutorial/05_opt_extracellular/Ch_extracellular.ipynb b/docs/tutorial/05_opt_extracellular/Ch_extracellular.ipynb new file mode 100644 index 00000000..d3b3a341 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/Ch_extracellular.ipynb @@ -0,0 +1,840 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "12cb39af-dc4e-4c43-8113-7c74b5f72562", + "metadata": {}, + "source": [ + "# Recording Extracellular Potentials and Current Source Densities" + ] + }, + { + "cell_type": "markdown", + "id": "9b01bb08-b268-4dcf-904f-56fdde5dbb2e", + "metadata": {}, + "source": [ + "Extracellular local field potential (LFP) recordings are among the most common electrophysiological recordings for studying brain function. Thus, many scientists might be interested in recording LFPs while simulating the activity of distinct brain areas to replicate or compare to in-vivo experimental recordings. This tutorial shows how to record extracellular LFPs with BioNet, modify the extracellular resistance, and calculate the current source density (CSD) generating the measured LFPs. \n", + "\n", + "**Note** - scripts and files for running this tutorial can be found in the directory [05_opt_extracellular/](https://github.com/AllenInstitute/bmtk/tree/develop/docs/tutorial/05_opt_extracellular)" + ] + }, + { + "cell_type": "markdown", + "id": "21999a38-59d7-4a7a-a2e6-a7aebfe6b528", + "metadata": {}, + "source": [ + "---\n", + "\n", + "## Contents\n", + "1. [Example: Recording Extracellular Local Field Potentials (LFPs)](#example-lfps)\n", + "2. [Example: Calculating the Current Source Density (CSD)](#example-cal-csd)\n", + "\n", + "\n", + "---" + ] + }, + { + "cell_type": "markdown", + "id": "8775ca0d-2a02-443c-926d-df0e4e9baadd", + "metadata": {}, + "source": [ + "## 1. Example: Recording Extracellular Local Field Potentials (LFPs) " + ] + }, + { + "cell_type": "markdown", + "id": "09f9cdc4-c577-4bad-95ce-435f3873338f", + "metadata": {}, + "source": [ + "A part of BioNet's built-in functionality is the ability to record extracellular LFPs from morphologically detailed networks of cells. Like with in-vivo experiments, it involves placing one or more electrodes by the cells that will pick up the change of the extracellular potentials throughout the experiment/simulation. This can be useful for comparing simulations to actual experiment recordings taken from an M.E.A., neuronal probe, ECoG, or EEG. It can also be used to calculate the CSD, thus capturing the slow-reacting, non-spiking dynamics of your network.\n", + "\n", + "**Note:** BMTK only supports capturing the LFPs for `biophysical` **model_type** cells. It will not work in PointNet since these are point-neuron cells, which cannot directly generate extracellular potentials - generated by spatially separated transmembrane currents. Nor will it work with BioNet/NEURON `point-neuron` models. If a network contains a mix of `biophysical` and `point-neuron` models, the `point-neuron` models will be ignored when collecting extracellular recordings.\n", + "\n", + "Also, **NOTE** that adding extracellular potential recordings to your network is computationally expensive. Expect simulations to take significantly longer than if you were just recording spikes or membrane voltages.\n" + ] + }, + { + "cell_type": "markdown", + "id": "6b8afc8f-dda6-4022-91ab-4084366a734a", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### Step 1: Building a Network (Caveats)\n", + "\n", + "Creating a network that is capable of recording LFPs is no different than the way you would normally create a SONATA-based network. And most of the time, we can dynamically add a recording electrode before any simulation. However, there are some requirements that we must be cognizant of:\n", + "\n", + "1. Our network **MUST** have attributes **x**, **y**, **z** defined for all cells being recorded by our electrode (s). Variables like spiking events or membrane potential are spatially invariant, so the exact coordinates of each cell of the network are not required. With extracellular recordings, the distance between the cells and electrodes will be very important in determining LFP.\n", + "2. You should also take care that **rotation_angle_xaxis**, **rotation_angle_yaxis**, and **rotation_angle_zaxis** are set properly. Especially if the cell is bipolar-like in morphology, it can make a big difference. However, if you want to use the default morphology from the *swc*, *asc*, *hoc*, etc, then not specifying the **rotation_angles** will just default rotations to 0.0\n", + "\n", + "In this example, we build our network cells like such:\n", + "```python\n", + "x, y, z = get_coords(20, y_range=[-300, -100])\n", + "cortcol.add_nodes(\n", + " N=20,\n", + "\n", + " # Reserved SONATA keywords used during simulation\n", + " model_type='biophysical',\n", + " model_template='ctdb:Biophys1.hoc',\n", + " model_processing='aibs_perisomatic',\n", + " dynamics_params='Scnn1a_485510712_params.json',\n", + " morphology='Scnn1a_485510712_morphology.swc',\n", + "\n", + " # The x, y, z locations and orientations (in Euler angles) of each cell\n", + " # Here, rotation around the pia-to-white-matter axis is randomized\n", + " x=x,\n", + " y=y,\n", + " z=z,\n", + " rotation_angle_xaxis=0,\n", + " rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20),\n", + " rotation_angle_zaxis=3.646878266,\n", + "\n", + " tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False),\n", + " layer='L23',\n", + " model_name='Scnn1a',\n", + " ei='e'\n", + ")\n", + "```\n", + "\n", + "In this particular example, we use a very simplified \"cortical column\" consisting of layers `L2/3`, `L4` (which receives most of the network inputs), and `L5` pyramidal cells surrounded by inhibitory interneurons. The cells are built in a column parallel to the **y** axis and centered at **x=0** and **z=0**. \n", + "\n", + "We can use VND to visualize our network to make sure the layering and rotation of our cells are correct:\n", + "\n", + "
\n", + "
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\n", + "\n", + "The full script for this example can be found in the file *build_network.lfp.py*. We encourage you to make changes and test out the effects different cell types, synapses, and geometry have on LFP recordings. You can also try using a different network altogether.\n" + ] + }, + { + "cell_type": "markdown", + "id": "1d273e38-7c3a-46c0-aeea-ae399859d55e", + "metadata": {}, + "source": [ + "### Step 2: Adding \"ecp\" recordings to simulation config.\n", + "\n", + "The next step is to open up our SONATA simulation configuration file (*config.lfp.json*) so that it contains the following in the \"reports\" section.\n", + "\n", + "```json\n", + "\"reports\": {\n", + " \"cortical_electrode\": {\n", + " \"module\": \"extracellular\",\n", + " \"variable_name\": \"v\",\n", + " \"cells\": \"all\",\n", + " \"electrode_positions\": \"./components/electrodes/linear_electrode.csv\",\n", + " \"electrode_channels\": \"all\"\n", + " }\n", + "}\n", + "```\n", + "* The **module** should always be set to value `extracellular`, which tells BMTK what type of recording to apply.\n", + "* The **variable_name** should be set to `v`, which is the voltage just outside of every compartment membrane.\n", + "* Setting **cells** to all tells BMTK to record the extracellular potential of all (biophysical) cells in the model. You can use the [node_set]() to select only a portion of cells to record from. For example, if you wanted to filter by specific node-ids:\n", + "```json\n", + " \"cells\": {\n", + " \"population\": \"cortcol\",\n", + " \"node_ids\": [0, 100, 200, 300]\n", + " },\n", + "```\n", + "     or if you wanted to record from Layer 4 excitatory cells\n", + "```json\n", + " \"cells\": {\n", + " \"layer\": \"L4\",\n", + " \"ei\": \"e\"\n", + " },\n", + "```\n", + "* **electrode_positions** will point to the csv file containing recording electrodes (see below)\n", + "* We set **electrode_channels** to `all` to indicate the use of all possible electrodes in the electrodes csv file.\n", + "\n" + ] + }, + { + "cell_type": "markdown", + "id": "d54bc46d-7fca-4a9f-8b06-7ec73fcd1d78", + "metadata": {}, + "source": [ + "### Step 3: Creating electrodes file\n", + "\n", + "We must tell BioNet the location of all recording electrodes, which we specify in the file *./componets/electrodes/linear_electrodes.csv*. This is a simple space-separated file where each line represents the `channel_id`, `x`, `y`, and `z` position for each channel that will be recording ecp from." + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "3808f6c8-38bd-4779-bfd8-95be97b3df12", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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" + ], + "text/plain": [ + " channel x_pos y_pos z_pos\n", + "0 0 0.0 0.0 0.0\n", + "1 1 0.0 -100.0 0.0\n", + "2 2 0.0 -200.0 0.0\n", + "3 3 0.0 -300.0 0.0\n", + "4 4 0.0 -400.0 0.0\n", + "5 5 0.0 -500.0 0.0\n", + "6 6 0.0 -600.0 0.0\n", + "7 7 0.0 -700.0 0.0\n", + "8 8 0.0 -800.0 0.0" + ] + }, + "execution_count": 1, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "import pandas as pd\n", + "\n", + "\n", + "pd.read_csv('components/electrodes/linear_electrode.csv', sep=' ')" + ] + }, + { + "cell_type": "markdown", + "id": "b1dbd11b-d29b-4b01-b414-70a6038fdca6", + "metadata": {}, + "source": [ + "In this example, an electrode array placed straight down the column will record the LFP at every 100-micron interval. Like with the placement of cells, we can use whatever coordinate framework for placing our network. In this case, we assume our column is centered at the axis. However, we could also use a global framework like the [CCF](https://biccn.org/standards/common-coordinate-frameworks-biccn). The important thing for BMTK is that the electrodes and cells must be in the same coordinate system. " + ] + }, + { + "cell_type": "markdown", + "id": "0c83436c-a787-4286-99cd-cdb3f72117c4", + "metadata": {}, + "source": [ + "### Step 4: Running the simulation\n", + "\n", + "Now that we've updated our simulation configuration accordingly, we can run the simulation like we normally would (be mindful that it will likely take longer and use more resources than normal):\n" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "0ab3c735-c03b-419c-bf91-0ebb6019e3a6", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "/home/shared/workspace/docs/tutorial/05_opt_extracellular/components/mechanisms\n", + "Mod files: \"modfiles/CaDynamics.mod\" \"modfiles/Ca_HVA.mod\" \"modfiles/Ca_LVA.mod\" \"modfiles/Ih.mod\" \"modfiles/Im.mod\" \"modfiles/Im_v2.mod\" \"modfiles/K_P.mod\" \"modfiles/K_T.mod\" \"modfiles/Kd.mod\" \"modfiles/Kv2like.mod\" \"modfiles/Kv3_1.mod\" \"modfiles/NaTa.mod\" \"modfiles/NaTs.mod\" \"modfiles/NaV.mod\" \"modfiles/Nap.mod\" \"modfiles/SK.mod\" \"modfiles/exp1isyn.mod\" \"modfiles/exp1syn.mod\" \"modfiles/stp1syn.mod\" \"modfiles/stp2syn.mod\" \"modfiles/stp3syn.mod\" \"modfiles/stp4syn.mod\" \"modfiles/stp5isyn.mod\" \"modfiles/stp5syn.mod\" \"modfiles/vecevent.mod\"\n", + "\n", + "COBJS=''\n", + " -> \u001b[32mCompiling\u001b[0m mod_func.c\n", + "gcc -O2 -I. -I/opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/include -I/nrnwheel/openmpi/include -fPIC -c mod_func.c -o mod_func.o\n", + " => \u001b[32mLINKING\u001b[0m shared library ./libnrnmech.so\n", + "g++ -O2 -DVERSION_INFO='8.0.0' -std=c++11 -shared -fPIC -I /opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/include -o ./libnrnmech.so -Wl,-soname,libnrnmech.so \\\n", + " ./mod_func.o ./CaDynamics.o ./Ca_HVA.o ./Ca_LVA.o ./Ih.o ./Im.o ./Im_v2.o ./K_P.o ./K_T.o ./Kd.o ./Kv2like.o ./Kv3_1.o ./NaTa.o ./NaTs.o ./NaV.o ./Nap.o ./SK.o ./exp1isyn.o ./exp1syn.o ./stp1syn.o ./stp2syn.o ./stp3syn.o ./stp4syn.o ./stp5isyn.o ./stp5syn.o ./vecevent.o -L/opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/lib -lnrniv -Wl,-rpath,/opt/conda/envs/bmtk/lib/python3.9/site-packages/neuron/.data/lib \n", + "rm -f ./.libs/libnrnmech.so ; mkdir -p ./.libs ; cp ./libnrnmech.so ./.libs/libnrnmech.so\n", + "Successfully created x86_64/special\n" + ] + } + ], + "source": [ + "! cd components/mechanisms && nrnivmodl modfiles" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "314e78ab-ef2e-4b71-9b31-e67124d3b012", + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "Warning: no DISPLAY environment variable.\n", + "--No graphics will be displayed.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "2024-10-21 17:26:00,659 [INFO] Created log file\n", + "2024-10-21 17:26:00,876 [INFO] Building cells.\n", + "2024-10-21 17:26:04,530 [INFO] Building recurrent connections\n", + "2024-10-21 17:26:05,813 [INFO] Building virtual cell stimulations for virt_exc_spikes\n", + "2024-10-21 17:26:08,413 [INFO] Running simulation for 2000.000 ms with the time step 0.100 ms\n", + "2024-10-21 17:26:08,414 [INFO] Starting timestep: 0 at t_sim: 0.000 ms\n", + "2024-10-21 17:26:08,414 [INFO] Block save every 5000 steps\n", + "2024-10-21 17:26:53,054 [INFO] step:5000 t_sim:500.00 ms\n", + "2024-10-21 17:27:38,462 [INFO] step:10000 t_sim:1000.00 ms\n", + "2024-10-21 17:28:26,600 [INFO] step:15000 t_sim:1500.00 ms\n", + "2024-10-21 17:29:10,021 [INFO] step:20000 t_sim:2000.00 ms\n", + "2024-10-21 17:29:10,085 [INFO] Simulation completed in 3.0 minutes, 1.672 seconds \n" + ] + } + ], + "source": [ + "from bmtk.simulator import bionet\n", + "\n", + "bionet.reset()\n", + "conf = bionet.Config.from_json('config.lfp.json')\n", + "conf.build_env()\n", + "\n", + "net = bionet.BioNetwork.from_config(conf)\n", + "sim = bionet.BioSimulator.from_config(conf, network=net)\n", + "sim.run()" + ] + }, + { + "cell_type": "markdown", + "id": "505e2b14-1f8b-49e6-8324-550a71a8929d", + "metadata": {}, + "source": [ + "### Step 5: Analyzing the results\n", + "\n", + "The `bmtk.analyzer.ecp` package of BMTK includes a number of tools for analyzing and visualizing the results of the electrode recordings:\n" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "d58f72c7-73a5-46b8-aad9-f616f55708c9", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "from bmtk.analyzer.ecp import plot_ecp\n", + "\n", + "_ = plot_ecp(config_file='config.lfp.json', report_name='cortical_electrode')" + ] + }, + { + "cell_type": "markdown", + "id": "502259b2-a07c-4b37-8343-606d47d5c9b3", + "metadata": {}, + "source": [ + "But if you need to analyze the results yourself, they will be saved in the *output/cortical_electrode.h5* file, which is an HDF5 following SONATA format for [Extracellular reports](https://github.com/AllenInstitute/sonata/blob/master/docs/SONATA_DEVELOPER_GUIDE.md#extracellular-report). The results are stored in the `/ecp/data` table that is $T-timestamps x N-channels$ in size.\n", + "\n", + "\n", + "
\n", + "
\n", + " \n", + "
\n", + "
\n" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "99ce85b5-106a-4f31-a1c7-795c6f401235", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "import h5py\n", + "import numpy as np\n", + "import matplotlib.pyplot as plt\n", + "\n", + "channel_id = 5\n", + "\n", + "with h5py.File('output_lfp/cortical_electrode.h5', 'r') as h5:\n", + " channel_idx = np.argwhere(h5['/ecp/channel_id'][()] == channel_id).flatten()\n", + " ts = np.arange(start=h5['/ecp/time'][0], stop=h5['/ecp/time'][1], step=h5['/ecp/time'][2])\n", + " plt.plot(ts, h5['/ecp/data'][:, channel_idx])\n", + " plt.title(f'Channel {channel_id}')\n", + " plt.xlabel(h5['/ecp/time'].attrs['units'])\n", + " plt.ylabel(h5['/ecp/data'].attrs['units'])" + ] + }, + { + "cell_type": "markdown", + "id": "db84d250-afcd-4bf2-a736-56ff816ba219", + "metadata": {}, + "source": [ + "### (Optional) Finding individual contributions\n", + "\n", + "When each channel in the electrode calculates the ECP at each given time, it does so by taking the extracellular Vm of each cell, adjusting for distance and interstitual resistance, and summing the results over all recorded cells. \n", + "\n", + "Sometimes, for deeper analysis of a simulation, you may want to measure the individual contribution each cell has on the electrode. To do so in our simulation we just need to add the **contributions_dir** option to the simulation configuration:\n", + "\n", + "```json\n", + "\"reports\": {\n", + " \"cortical_electrode\": {\n", + " \"module\": \"extracellular\",\n", + " \"variable_name\": \"v\",\n", + " \"cells\": \"all\",\n", + " \"electrode_positions\": \"./components/electrodes/linear_electrode.csv\",\n", + " \"electrode_channels\": \"all\",\n", + " \"contributions_dir\": \"cell_contributions\"\n", + " }\n", + "}\n", + "```\n", + "\n", + "Now, when you run the simulation, it will add an extra directory *output_lfp/cell_contributions/* that will contain N SONATA Extracellular reports for each N individual cell (The file name of each h5 will correspond to the node_id of each cell).\n", + "\n", + "Be **WARNED** that adding the \"contributions_dir\" option will take up a lot of disk space for even moderately sized networks. Feel free to edit *config.lfp.json* and see how different cells and cell-types differ in their LFP signatures.\n" + ] + }, + { + "cell_type": "markdown", + "id": "eda4f2e5-0c31-4e44-97fe-cdccfa476944", + "metadata": {}, + "source": [ + "## 2. Example: Calculating the Current Source Density (CSD) " + ] + }, + { + "cell_type": "markdown", + "id": "67133568-2105-47cc-8f30-056949ebe155", + "metadata": {}, + "source": [ + "Scientists are often interested in estimating the current sources or CSD generating the measured LFPs. Calculating the CSD associated with simulated LFPs can also be useful for comparing simulations to experimental data. This example demonstrates how to compute the CSD from the simulated LFPs in the first example using the iCSD method from [Pettersen et al. 2006](https://doi.org/10.1016/j.jneumeth.2005.12.005). The iCSD methods can be applied to data recorded from multielectrode arrays with various geometries.\n", + "\n", + "iCSD methods are available in the py-iCSD toolbox, which is a translation of the core functionality of the CSDplotter MATLAB package to Python. The py-ICSD toolbox can be downloaded from https://github.com/espenhgn/iCSD" + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "id": "d9ac57ad-a270-49ac-ada1-969a37994bde", + "metadata": {}, + "outputs": [], + "source": [ + "import sys\n", + "sys.path.append('icsd_scripts/')" + ] + }, + { + "cell_type": "markdown", + "id": "44016e87-e584-460b-97f3-0d194bd97e69", + "metadata": {}, + "source": [ + "For the below scripts you will also need to install third party packages [quantities](https://github.com/python-quantities/python-quantities) and [neo](https://github.com/NeuralEnsemble/python-neo) to handle dimensional units and electrophysiology calculations, respectively" + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "id": "37b5bd38-150b-4d49-b967-3a8ecfc3182c", + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "/home/shared/workspace/docs/tutorial/05_opt_extracellular/icsd_scripts/icsd.py:1292: SyntaxWarning: \"is not\" with a literal. Did you mean \"!=\"?\n", + " if f_type is not \"identity\" and f_order is None:\n" + ] + } + ], + "source": [ + "import icsd\n", + "import quantities as pq" + ] + }, + { + "cell_type": "markdown", + "id": "150dca73-2942-49d1-afeb-002af0db546f", + "metadata": {}, + "source": [ + "### Step 1: Setting up quantities for py-iCSD toolbox\n", + "\n", + "All inputs to the iCSD methods class instances are considered Quantity arrays. First, we need to patch quantities with the SI unit Siemens if it does not exist:" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "id": "eb319516-83b4-44fa-83f3-0df017eb4eb6", + "metadata": {}, + "outputs": [], + "source": [ + "for symbol, prefix, definition, u_symbol in zip(\n", + " ['siemens', 'S', 'mS', 'uS', 'nS', 'pS'],\n", + " ['', '', 'milli', 'micro', 'nano', 'pico'],\n", + " [pq.A/pq.V, pq.A/pq.V, 'S', 'mS', 'uS', 'nS'],\n", + " [None, None, None, None, u'µS', None]):\n", + " if type(definition) is str:\n", + " definition = lastdefinition / 1000\n", + " if not hasattr(pq, symbol):\n", + " setattr(pq, symbol, pq.UnitQuantity(\n", + " prefix + 'siemens',\n", + " definition,\n", + " symbol=symbol,\n", + " u_symbol=u_symbol))\n", + " lastdefinition = definition" + ] + }, + { + "cell_type": "markdown", + "id": "dd40b27a-7501-4885-8f2b-f7480dd0383a", + "metadata": {}, + "source": [ + "### Step 2: Preparing LFP data and defining parameters for the CSD calculation\n", + "\n", + "The iCSD method considers the CSD to have cylindrical symmetry with a diameter `diam` specified by the user based on the geometry of your simulated network or the area captured by your experimental recordings. Here, we calculate the CSD assuming a homogenous conductivity `sigma` throughout the column and an infinite medium (i.e., the extracellular conductivity above the cortical surface is the same as the grey matter conductivity - `sigma_top = sigma`). However, you can use a different value for `sigma_top` if you use substances such as saline (with a high conductivity) or oil (with a very low conductivity) to prevent the brain from drying during your experiments.\n", + "\n", + "In addition to these parameters, you need to input the LFP data `lfp` with units in SI, the coordinates of the electrodes `coord_electrode`, and the type `f_type` and order `f_order` of the spatial filter to be applied to the estimated CSD. You must append the corresponding quantities to `lfp`, `coord_electrode`, `diam`, `sigma`, and `sigma_top` to convert them to Quantity arrays.\n", + "\n", + "Here, we will use the delta-iCSD method for calculating the CSD, using a Gaussian 3-point filter with a standard deviation equal to 1 (`sigma = 1`). However, the py-iCSD toolbox also includes the step-iCSD, spline-iCSD, and std or standard CSD methods. More details on these methods can be found in [Pettersen et al. 2006](https://doi.org/10.1016/j.jneumeth.2005.12.005). \n", + "\n", + "These are examples of the input dictionaries for each method:\n", + "```python\n", + "delta_input = {\n", + " 'lfp' : lfp_data,\n", + " 'coord_electrode' : z_data,\n", + " 'diam' : diam, # source diameter\n", + " 'sigma' : sigma, # extracellular conductivity\n", + " 'sigma_top' : sigma, # conductivity on top of cortex\n", + " 'f_type' : 'gaussian', # gaussian filter\n", + " 'f_order' : (3, 1), # 3-point filter, sigma = 1.\n", + "}\n", + "step_input = {\n", + " 'lfp' : lfp_data,\n", + " 'coord_electrode' : z_data,\n", + " 'diam' : diam,\n", + " 'h' : h, # source thickness\n", + " 'sigma' : sigma,\n", + " 'sigma_top' : sigma,\n", + " 'tol' : 1E-12, # Tolerance in numerical integration\n", + " 'f_type' : 'gaussian',\n", + " 'f_order' : (3, 1),\n", + "}\n", + "spline_input = {\n", + " 'lfp' : lfp_data,\n", + " 'coord_electrode' : z_data,\n", + " 'diam' : diam,\n", + " 'sigma' : sigma,\n", + " 'sigma_top' : sigma,\n", + " 'num_steps' : 201, # Spatial CSD upsampling to N steps\n", + " 'tol' : 1E-12,\n", + " 'f_type' : 'gaussian',\n", + " 'f_order' : (20, 5),\n", + "}\n", + "std_input = {\n", + " 'lfp' : lfp_data,\n", + " 'coord_electrode' : z_data,\n", + " 'sigma' : sigma,\n", + " 'f_type' : 'gaussian',\n", + " 'f_order' : (3, 1),\n", + "}\n", + "```" + ] + }, + { + "cell_type": "markdown", + "id": "ede35e49-ab83-40fe-9d8d-a8cbec883e99", + "metadata": {}, + "source": [ + "Getting the simulated LFPs and electrodes' coordinates from the first example:" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "0116042e-249d-45a2-9e39-23dbb99f9e96", + "metadata": {}, + "outputs": [], + "source": [ + "import h5py\n", + "\n", + "with h5py.File('output_lfp/cortical_electrode.h5', 'r') as h5:\n", + " channel_idx = h5['/ecp/channel_id'][()]\n", + " ts = np.arange(start=h5['/ecp/time'][0], stop=h5['/ecp/time'][1], step=h5['/ecp/time'][2])\n", + " lfp = h5['/ecp/data'][()]\n", + "\n", + "\n", + "coord_ele = pd.read_csv('components/electrodes/linear_electrode.csv', sep=' ')" + ] + }, + { + "cell_type": "markdown", + "id": "5bb82f49-177e-40d0-9a43-39e7ea17c490", + "metadata": {}, + "source": [ + "Defining the input dictionary for the delta-iCSD method: " + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "id": "b4ae6192-4565-4503-9568-8bbd68ce5c73", + "metadata": {}, + "outputs": [], + "source": [ + "z_data = np.array(coord_ele['y_pos']) * (-1E-6) * pq.m # [um] --> [m] linear probe - electrodes' position in depth\n", + "diam = 800E-6 * pq.m # [m] source diameter\n", + "h = 100E-6 * pq.m # [m] distance between channels\n", + "sigma = 0.3* pq.S / pq.m # [S/m] or [1/(ohm*m)] extracellular conductivity\n", + "sigma_top = 0.3* pq.S / pq.m # [S/m] or [1/(ohm*m)] conductivity on top of cortex\n", + "\n", + "delta_input = {\n", + " 'lfp' : lfp.T*1E-3*pq.V, #[um] --> [V]\n", + " 'coord_electrode' : z_data,\n", + " 'diam' : diam,\n", + " 'sigma' : sigma,\n", + " 'sigma_top' : sigma_top,\n", + " 'f_type' : 'gaussian', # gaussian filter\n", + " 'f_order' : (3, 1), # 3-point filter, sigma = 1.\n", + " }" + ] + }, + { + "cell_type": "markdown", + "id": "f520d1cf-32f5-427f-802c-c4f3771e3b30", + "metadata": {}, + "source": [ + "### Step 3: Calculating the CSD\n", + "\n", + "First, we create the delta CSD-method class instance:\n", + "```python\n", + "csd_dict = dict(\n", + " delta_icsd = icsd.DeltaiCSD(**delta_input),\n", + ")\n", + "```\n", + "\n", + "The class methods get_csd() and filter_csd() are used below to get the raw and spatially filtered versions of the CSD estimates. The raw- and filtered CSD estimates are returned as Quantity arrays. \n", + "\n", + "In the comments below, we illustrate how to calculate the CSD using the other methods, considering their input dictionary is defined as shown in Step 2.\n" + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "4fb517ab-9be2-48e8-b03c-6982c91058e4", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "discrete filter coefficients: \n", + "b = [ 0.607 1.000 0.607 ], \n", + "a = [ 2.213 ]\n" + ] + } + ], + "source": [ + "csd_dict = dict(\n", + " delta_icsd = icsd.DeltaiCSD(**delta_input),\n", + "\n", + " #Other methods for computing CSD:\n", + " #step_icsd = icsd.StepiCSD(**step_input),\n", + " #spline_icsd = icsd.SplineiCSD(**spline_input),\n", + " #std_csd = icsd.StandardCSD(**std_input),\n", + ")\n", + "\n", + "for method, csd_obj in list(csd_dict.items()):\n", + " csd_raw = csd_obj.get_csd() # num_channels x trial_duration\n", + " csd_smooth = csd_obj.filter_csd(csd_raw) # num_channels x trial_duration" + ] + }, + { + "cell_type": "code", + "execution_count": 12, + "id": "0ba20d00-f906-479d-ad34-d3fc175e97b1", + "metadata": {}, + "outputs": [ + { + "data": { + "text/plain": [ + "Text(0.5, 0, 'timestep')" + ] + }, + "execution_count": 12, + "metadata": {}, + "output_type": "execute_result" + }, + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "fig, axes = plt.subplots(3,1, figsize=(8,8))\n", + "\n", + "lfp_data = lfp.T*1E-3*pq.V\n", + "\n", + "#plot LFP signal\n", + "ax = axes[0]\n", + "im = ax.imshow(np.array(lfp_data), origin='upper', vmin=-abs(lfp_data).max(), \\\n", + " vmax=abs(lfp_data).max(), cmap='bwr')\n", + "ax.axis(ax.axis('tight'))\n", + "cb = plt.colorbar(im, ax=ax)\n", + "cb.set_label('LFP (%s)' % lfp_data.dimensionality.string)\n", + "ax.set_xticklabels([])\n", + "ax.set_title('LFP')\n", + "ax.set_ylabel('ch #')\n", + "\n", + "#plot raw csd estimate\n", + "ax = axes[1]\n", + "im = ax.imshow(np.array(csd_raw), origin='upper', vmin=-abs(csd_raw).max(), \\\n", + " vmax=abs(csd_raw).max(), cmap='jet_r')\n", + "ax.axis(ax.axis('tight'))\n", + "ax.set_title(csd_obj.name)\n", + "cb = plt.colorbar(im, ax=ax)\n", + "cb.set_label('CSD (%s)' % csd_raw.dimensionality.string)\n", + "ax.set_xticklabels([])\n", + "ax.set_ylabel('ch #')\n", + "\n", + "#plot spatially filtered csd estimate\n", + "ax = axes[2]\n", + "im = ax.imshow(np.array(csd_smooth), origin='upper', vmin=-abs(csd_smooth).max(), \\\n", + " vmax=abs(csd_smooth).max(), cmap='jet_r')\n", + "ax.axis(ax.axis('tight'))\n", + "ax.set_title(csd_obj.name + ', filtered')\n", + "cb = plt.colorbar(im, ax=ax)\n", + "cb.set_label('CSD (%s)' % csd_smooth.dimensionality.string)\n", + "ax.set_ylabel('ch #')\n", + "ax.set_xlabel('timestep')" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "e4e75331-bb64-409e-aadc-6945c799d5a1", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3 (ipykernel)", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.9.7" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/05_opt_extracellular/_static/_tutorial_images/hdf5_ecp_format.png b/docs/tutorial/05_opt_extracellular/_static/_tutorial_images/hdf5_ecp_format.png new file mode 100755 index 00000000..35529ab1 Binary files /dev/null and b/docs/tutorial/05_opt_extracellular/_static/_tutorial_images/hdf5_ecp_format.png differ diff --git a/docs/tutorial/05_opt_extracellular/_static/_tutorial_images/lfp_cortcol_small.png b/docs/tutorial/05_opt_extracellular/_static/_tutorial_images/lfp_cortcol_small.png new file mode 100755 index 00000000..f7b45e33 Binary files /dev/null and b/docs/tutorial/05_opt_extracellular/_static/_tutorial_images/lfp_cortcol_small.png differ diff --git a/docs/tutorial/05_opt_extracellular/build_network.lfp.py b/docs/tutorial/05_opt_extracellular/build_network.lfp.py new file mode 100644 index 00000000..889811ae --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/build_network.lfp.py @@ -0,0 +1,626 @@ +import numpy as np + +from bmtk.builder.networks import NetworkBuilder +from bmtk.builder.bionet import rand_syn_locations + +# L2/3 - 150 - 300 +# L4 - 300 - 400 +# L5 - 400 - 550 +def get_coords(N, radius_min=0.0, radius_max=400.0, y_range=[300, 400]): + phi = 2.0 * np.pi * np.random.random([N]) + r = np.sqrt((radius_min ** 2 - radius_max ** 2) * np.random.random([N]) + radius_max ** 2) + x = r * np.cos(phi) + y = np.random.uniform(y_range[0], y_range[1], size=N) + z = r * np.sin(phi) + return x, y, z + + +# def generate_coords_plane(ncells, size_x=240.0, size_y=120.0): +# xs = np.random.uniform(0.0, size_x, ncells) +# ys = np.random.uniform(0.0, size_y, ncells) +# return xs, ys + + +# def exc_exc_rule(source, target, max_syns): +# """Connect rule for exc-->exc neurons, should return an integer 0 or greater""" +# if source['node_id'] == target['node_id']: +# # prevent a cell from synapsing with itself +# return 0 + +# # calculate the distance between tuning angles and use it to choose +# # number of connections using a binomial distribution. +# src_tuning = source['tuning_angle'] +# trg_tuning = target['tuning_angle'] +# tuning_dist = np.abs((src_tuning - trg_tuning + 180) % 360 - 180) +# probs = 1.0 - (np.max((tuning_dist, 10.0)) / 180.0) +# return np.random.binomial(n=max_syns, p=probs) + + +# def others_conn_rule(source, target, max_syns, max_distance=300.0, sigma=60.0): +# if source['node_id'] == target['node_id']: +# return 0 + +# dist = np.sqrt((source['x'] - target['x']) ** 2 + (source['z'] - target['z']) ** 2) +# if dist > max_distance: +# return 0 + +# prob = np.exp(-(dist / sigma) ** 2) +# return np.random.binomial(n=max_syns, p=prob) + + +# def connect_lgn_cells(source, targets, max_targets, min_syns=1, max_syns=15, lgn_size=(240, 120), +# l4_radius=400.0, ellipse=(100.0, 500.0)): +# # Map the lgn cells from the plane to the circle +# x, y = source['x'], source['y'] +# x = x / lgn_size[0] - 0.5 +# y = y / lgn_size[1] - 0.5 +# src_x = x * np.sqrt(1.0 - (y**2/2.0)) * l4_radius +# src_y = y * np.sqrt(1.0 - (x**2/2.0)) * l4_radius + +# # Find (the indices) of all the target cells that are within the given ellipse, if there are more than max_targets +# # then randomly choose them +# a, b = ellipse[0]**2, ellipse[1]**2 +# dists = [(src_x-t['x'])**2/a + (src_y-t['y'])**2/b for t in targets] +# valid_targets = np.argwhere(np.array(dists) <= 1.0).flatten() +# if len(valid_targets) > max_targets: +# valid_targets = np.random.choice(valid_targets, size=max_targets, replace=False) + +# # Create an array of all synapse count. Most targets with have 0 connection, except for the "valid_targets" which +# # will have between [min_syns, max_syns] number of connections. +# nsyns_arr = np.zeros(len(targets), dtype=int) +# for idx in valid_targets: +# nsyns_arr[idx] = np.random.randint(min_syns, max_syns) + +# return nsyns_arr + + +cortcol = NetworkBuilder('cortcol') + +x, y, z = get_coords(20, y_range=[-300, -100]) +cortcol.add_nodes( + N=20, + + # Reserved SONATA keywords used during simulation + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_perisomatic', + dynamics_params='Scnn1a_485510712_params.json', + morphology='Scnn1a_485510712_morphology.swc', + + # The x, y, z locations and orientations (in Euler angles) of each cell + # Here, rotation around the pia-to-white-matter axis is randomized + x=x, + y=y, + z=z, + rotation_angle_xaxis=[0]*20, + rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20), + rotation_angle_zaxis=[3.646878266]*20, + + tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False), + layer='L23', + model_name='Scnn1a', + ei='e' +) + + +x, y, z = get_coords(20, y_range=[-400, -300]) +cortcol.add_nodes( + N=20, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_perisomatic', + dynamics_params='Scnn1a_485510712_params.json', + morphology='Scnn1a_485510712_morphology.swc', + + # The x, y, z locations and orientations (in Euler angles) of each cell + # Here, rotation around the pia-to-white-matter axis is randomized + x=x, + y=y, + z=z, + rotation_angle_xaxis=[0]*20, + rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20), + rotation_angle_zaxis=[3.646878266]*20, + + tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False), + layer='L4', + model_name='Scnn1a', + ei='e' +) + + +x, y, z = get_coords(20, y_range=[-600, -400]) +cortcol.add_nodes( + N=20, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_perisomatic', + dynamics_params='Scnn1a_485510712_params.json', + morphology='Scnn1a_485510712_morphology.swc', + x=x, + y=y, + z=z, + rotation_angle_xaxis=[0]*20, + rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20), + rotation_angle_zaxis=[3.646878266]*20, + + tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False), + layer='L5', + model_name='Scnn1a', + ei='e' +) + +x, y, z = get_coords(20, y_range=[-600, -100]) +cortcol.add_nodes( + N=20, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing='aibs_perisomatic', + dynamics_params='Pvalb_473862421_params.json', + morphology='Pvalb_473862421_morphology.swc', + x=x, + y=y, + z=z, + rotation_angle_xaxis=np.random.uniform(0.0, 2*np.pi, size=20), + rotation_angle_yaxis=np.random.uniform(0.0, 2*np.pi, size=20), + rotation_angle_zaxis=[2.539551891]*20, + model_name='Pvalb', + ei='i' +) + +cortcol.add_edges( + source={'ei': 'e'}, + target={'ei': 'e'}, + connection_rule=lambda *_: np.random.randint(10, 15), + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + syn_weight=6.0e-03, + delay=0.5, + target_sections=['somatic', 'basal', 'apical'], + distance_range=[2.0, 1.0e20] +) + +cortcol.add_edges( + # Exc --> Inh connections + source={'ei': 'e'}, + target={'ei': 'i'}, + connection_rule=lambda *_: np.random.randint(1, 10), + dynamics_params='AMPA_ExcToInh.json', + model_template='Exp2Syn', + syn_weight=0.002, + delay=3.0, + target_sections=['somatic', 'basal'], + distance_range=[0.0, 1.0e+20]) + +cortcol.add_edges( + # Inh --> Exc connections + source={'ei': 'i'}, + target={'ei': 'e'}, + + connection_rule=lambda *_: np.random.randint(0, 6), + dynamics_params='GABA_InhToExc.json', + model_template='Exp2Syn', + syn_weight=0.0002, + delay=2.0, + target_sections=['somatic', 'basal', 'apical'], + distance_range=[0.0, 50.0] +) + +cortcol.build() +cortcol.save(output_dir='network_lfp') + + +thalamus = NetworkBuilder('thalamus') +thalamus.add_nodes( + N=50, + model_type='virtual', + model_template='lgnmodel:tOFF_TF15', + x=np.random.uniform(0.0, 240.0, 50), + y=np.random.uniform(0.0, 120.0, 50), + spatial_size=1.0, + pop_name='tOFF', + dynamics_params='tOFF_TF15.json' +) +thalamus.add_nodes( + N=50, + model_type='virtual', + model_template='lgnmodel:tON', + x=np.random.uniform(0.0, 240.0, 50), + y=np.random.uniform(0.0, 120.0, 50), + spatial_size=1.0, + pop_name='tON', + dynamics_params='tON_TF8.json' +) +thalamus.add_edges( + source=thalamus.nodes(), + target=cortcol.nodes(ei='e', layer='L4'), + connection_rule=20, + # iterator='one_to_all', + dynamics_params='AMPA_ExcToExc.json', + model_template='Exp2Syn', + delay=2.0, + syn_weight=0.00041, + target_sections=['basal', 'apical', 'somatic'], + distance_range=[0.0, 50.0] +) +# lgn.add_edges( +# target=visp.nodes(ei='i'), source=lgn.nodes(), +# connection_rule=10, +# dynamics_params='AMPA_ExcToInh.json', +# model_template='Exp2Syn', +# delay=2.0, +# syn_weight=0.00095, +# target_sections=['basal', 'apical', 'somatic'], +# distance_range=[0.0, 1e+20] +# ) + + +thalamus.build() +thalamus.save(output_dir='network_lfp') + +# def create_l4(network_dir): +# l4 = NetworkBuilder('l4') + +# x, y, z = get_coords(20) +# l4.add_nodes( +# N=20, + +# # Reserved SONATA keywords used during simulation +# model_type='biophysical', +# model_template='ctdb:Biophys1.hoc', +# model_processing='aibs_perisomatic', +# dynamics_params='Scnn1a_485510712_params.json', +# morphology='Scnn1a_485510712_morphology.swc', + +# # The x, y, z locations and orientations (in Euler angles) of each cell +# # Here, rotation around the pia-to-white-matter axis is randomized +# x=x, +# y=y, +# z=z, +# rotation_angle_xaxis=0, +# rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20), +# rotation_angle_zaxis=3.646878266, + +# # Optional parameters +# tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False), +# model_name='Scnn1a', +# ei_type='e' +# ) +# x, y, z = get_coords(20) +# l4.add_nodes( +# # Rorb excitatory cells +# N=20, +# model_type='biophysical', +# model_template='ctdb:Biophys1.hoc', +# dynamics_params='Rorb_486509958_params.json', +# morphology='Rorb_486509958_morphology.swc', +# model_processing='aibs_perisomatic', + +# x=x, y=y, z=z, +# rotation_angle_xaxis=0, +# rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20), +# rotation_angle_zaxis=4.159763785, + +# model_name='Rorb', +# ei_type='e', +# tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False), +# ) + +# x, y, z = get_coords(20) +# l4.add_nodes( +# # Nr5a1 excitatory cells +# N=20, +# model_type='biophysical', +# model_template='ctdb:Biophys1.hoc', +# dynamics_params='Nr5a1_485507735_params.json', +# morphology='Nr5a1_485507735_morphology.swc', +# model_processing='aibs_perisomatic', + +# x=x, y=y, z=z, +# rotation_angle_xaxis=0, +# rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=20), +# rotation_angle_zaxis=4.159763785, + +# model_name='Nr5a1', +# ei_type='e', +# tuning_angle=np.linspace(start=0.0, stop=360.0, num=20, endpoint=False), +# ) + +# x, y, z = get_coords(15) +# l4.add_nodes( +# # Parvalbumin inhibitory cells, note these aren't assigned a tuning angle and ei=i +# N=15, + +# model_type='biophysical', +# model_template='ctdb:Biophys1.hoc', +# dynamics_params='Pvalb_473862421_params.json', +# morphology='Pvalb_473862421_morphology.swc', +# model_processing='aibs_perisomatic', + +# x=x, y=y, z=z, +# rotation_angle_xaxis=0, +# rotation_angle_yaxis=np.random.uniform(0.0, 2 * np.pi, size=15), +# rotation_angle_zaxis=2.539551891, + +# model_name='PValb', +# ei_type='i', +# ) + +# conns = l4.add_edges( +# # filter for subpopulation or source and target nodes +# source=l4.nodes(ei_type='e'), +# target=l4.nodes(ei_type='e'), + +# # connection function + any required parameters +# connection_rule=exc_exc_rule, +# connection_params={'max_syns': 15}, + +# # edge-type parameters +# syn_weight=3.0e-05, +# delay=2.0, +# dynamics_params='AMPA_ExcToExc.json', +# model_template='Exp2Syn', +# ) +# conns.add_properties( +# ['afferent_section_id', 'afferent_section_pos', 'afferent_swc_id', 'afferent_swc_pos'], +# rule=rand_syn_locations, +# rule_params={ +# 'sections': ['basal', 'apical'], +# 'distance_range': [30.0, 150.0], +# 'morphology_dir': 'components/morphologies' +# }, +# dtypes=[int, float, int, float] +# ) + +# ## Create e --> i connections +# conns = l4.add_edges( +# source=l4.nodes(ei_type='e'), +# target=l4.nodes(ei_type='i'), +# connection_rule=others_conn_rule, +# connection_params={'max_syns': 8}, +# syn_weight=0.0006, +# delay=2.0, +# dynamics_params='AMPA_ExcToInh.json', +# model_template='Exp2Syn', +# ) +# conns.add_properties( +# ['afferent_section_id', 'afferent_section_pos', 'afferent_swc_id', 'afferent_swc_pos'], +# rule=rand_syn_locations, +# rule_params={ +# 'sections': ['somatic', 'basal'], +# 'distance_range': [0.0, 1.0e+20], +# 'morphology_dir': 'components/morphologies' +# }, +# dtypes=[int, float, int, float] +# ) + +# ## Create i --> e connections +# conns = l4.add_edges( +# source=l4.nodes(ei_type='i'), +# target=l4.nodes(ei_type='e'), +# connection_rule=others_conn_rule, +# connection_params={'max_syns': 4}, +# syn_weight=0.07, +# delay=2.0, +# dynamics_params='GABA_InhToExc.json', +# model_template='Exp2Syn', +# ) +# conns.add_properties( +# ['afferent_section_id', 'afferent_section_pos', 'afferent_swc_id', 'afferent_swc_pos'], +# rule=rand_syn_locations, +# rule_params={ +# 'sections': ['somatic', 'basal', 'apical'], +# 'distance_range': [0.0, 50.0], +# 'morphology_dir': 'components/morphologies' +# }, +# dtypes=[int, float, int, float] +# ) + +# ## Create i --> i connections +# conns = l4.add_edges( +# source=l4.nodes(ei_type='i'), +# target=l4.nodes(ei_type='i'), +# connection_rule=others_conn_rule, +# connection_params={'max_syns': 4}, +# syn_weight=0.00015, +# delay=2.0, +# dynamics_params='GABA_InhToInh.json', +# model_template='Exp2Syn', +# ) +# conns.add_properties( +# ['afferent_section_id', 'afferent_section_pos', 'afferent_swc_id', 'afferent_swc_pos'], +# rule=rand_syn_locations, +# rule_params={ +# 'sections': ['somatic', 'basal', 'apical'], +# 'distance_range': [0.0, 1.0e+20], +# 'morphology_dir': 'components/morphologies' +# }, +# dtypes=[int, float, int, float] +# ) + +# l4.build() +# l4.save(output_dir='network') + +# return l4 + + +# def create_lgn(l4): +# lgn = NetworkBuilder('lgn') + +# x, y = generate_coords_plane(100) +# lgn.add_nodes( +# N=100, +# x=x, +# y=y, +# model_type='virtual', +# model_template='lgnmodel:tON', +# dynamics_params='tON_TF15.json', +# ei_type='e' +# ) + +# conns = lgn.add_edges( +# source=lgn.nodes(), +# target=l4.nodes(ei_type='e'), +# connection_rule=connect_lgn_cells, +# connection_params={'max_targets': 6}, +# iterator='one_to_all', +# model_template='Exp2Syn', +# dynamics_params='AMPA_ExcToExc.json', +# delay=2.0, +# syn_weight=0.0019 +# ) +# conns.add_properties( +# ['afferent_section_id', 'afferent_section_pos', 'afferent_swc_id', 'afferent_swc_pos'], +# rule=rand_syn_locations, +# rule_params={ +# 'sections': ['somatic', 'basal', 'apical'], +# 'distance_range': [0.0, 1.0e+20], +# 'morphology_dir': 'components/morphologies' +# }, +# dtypes=[int, float, int, float] +# ) + +# conns = lgn.add_edges( +# source=lgn.nodes(), +# target=l4.nodes(ei_type='i'), +# connection_rule=connect_lgn_cells, +# connection_params={'max_targets': 12, 'ellipse': (400.0, 400.0)}, +# iterator='one_to_all', +# model_template='Exp2Syn', +# dynamics_params='AMPA_ExcToInh.json', +# delay=2.0, +# syn_weight=0.003 +# ) +# conns.add_properties( +# ['afferent_section_id', 'afferent_section_pos', 'afferent_swc_id', 'afferent_swc_pos'], +# rule=rand_syn_locations, +# rule_params={ +# 'sections': ['somatic', 'basal', 'apical'], +# 'distance_range': [0.0, 1.0e+20], +# 'morphology_dir': 'components/morphologies' +# }, +# dtypes=[int, float, int, float] +# ) + +# lgn.build() +# lgn.save(output_dir='network') + + +# def create_lifs(l4): +# lif = NetworkBuilder('lif') + +# # place neurons on outer ring +# x, y, z = get_coords(80, radius_min=400.0, radius_max=800.0) +# lif.add_nodes( +# N=80, +# x=x, y=y, z=z, +# model_type='point_neuron', +# model_template='nrn:IntFire1', +# dynamics_params='IntFire1_exc_1.json', +# model_name='LIF_exc', +# ei_type='e', +# ) + +# x, y, z = get_coords(20, radius_min=400.0, radius_max=800.0) +# lif.add_nodes( +# N=20, +# x=x, y=y, z=z, +# model_type='point_neuron', +# model_template='nrn:IntFire1', +# dynamics_params='IntFire1_inh_1.json', +# model_name='LIF_inh', +# ei_type='i', +# ) + +# # Connect LIFs --> L4 +# lif.add_edges( +# source=lif.nodes(ei_type='e'), target=l4.nodes(ei_type='e'), +# connection_rule=8, +# syn_weight=0.015, +# delay=2.0, +# distance_range=[30.0, 150.0], +# target_sections=['somatic', 'basal', 'apical'], +# dynamics_params='AMPA_ExcToExc.json', +# model_template='Exp2Syn' +# ) + +# lif.add_edges( +# source=lif.nodes(ei_type='e'), target=l4.nodes(ei_type='i'), +# connection_rule=5, +# syn_weight=0.015, +# delay=2.0, +# distance_range=[30.0, 150.0], +# target_sections=['somatic', 'basal'], +# dynamics_params='AMPA_ExcToInh.json', +# model_template='Exp2Syn' +# ) + +# lif.add_edges( +# source=lif.nodes(ei_type='i'), target=l4.nodes(ei_type='e'), +# connection_rule=5, +# syn_weight=0.095, +# delay=2.0, +# distance_range=[0.0, 50.0], +# target_sections=['somatic', 'basal', 'apical'], +# dynamics_params='GABA_InhToExc.json', +# model_template='Exp2Syn' +# ) + +# lif.add_edges( +# source=lif.nodes(ei_type='i'), target=l4.nodes(ei_type='i'), +# connection_rule=5, +# syn_weight=0.095, +# delay=2.0, +# distance_range=[0.0, 1e+20], +# target_sections=['somatic', 'basal'], +# dynamics_params='GABA_InhToInh.json', +# model_template='Exp2Syn' +# ) + +# # Connect L4 --> LIFs +# lif.add_edges( +# source=l4.nodes(ei_type='e'), target=lif.nodes(), +# connection_rule=lambda *_: np.random.randint(0, 12), +# syn_weight=0.015, +# delay=2.0, +# dynamics_params='instantaneousExc.json', +# model_template='Exp2Syn' +# ) + +# lif.add_edges( +# source=l4.nodes(ei_type='i'), target=lif.nodes(), +# connection_rule=lambda *_: np.random.randint(0, 12), +# syn_weight=0.05, +# delay=2.0, +# dynamics_params='instantaneousInh.json', +# model_template='Exp2Syn' +# ) + +# # Connect LIFs --> LIFs +# lif.add_edges( +# source=lif.nodes(ei_type='e'), target=lif.nodes(), +# connection_rule=lambda *_: np.random.randint(0, 12), +# syn_weight=0.005, +# delay=2.0, +# dynamics_params='instantaneousExc.json', +# model_template='Exp2Syn' +# ) + +# lif.add_edges( +# source=lif.nodes(ei_type='i'), target=lif.nodes(), +# connection_rule=lambda *_: np.random.randint(0, 12), +# syn_weight=0.020, +# delay=2.0, +# dynamics_params='instantaneousInh.json', +# model_template='Exp2Syn' +# ) + + +# if __name__ == '__main__': +# print('Building l4') +# l4 = create_l4('network') + +# print('Building lgn') +# create_lgn(l4) + +# print('Building lifs') +# create_lifs(l4) \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Nr5a1_485507735_params.json b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Nr5a1_485507735_params.json new file mode 100644 index 00000000..c5cd60b1 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Nr5a1_485507735_params.json @@ -0,0 +1,148 @@ +{ + "passive": [ + { + "ra": 324.007874602, + "cm": [ + { + "section": "soma", + "cm": 1.0 + }, + { + "section": "axon", + "cm": 1.0 + }, + { + "section": "dend", + "cm": 2.750071758412201 + }, + { + "section": "apic", + "cm": 2.750071758412201 + } + ], + "e_pas": -95.39225196838379 + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 66, + 67, + 68, + 69 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -95.39225196838379 + } + ], + "genome": [ + { + "section": "soma", + "name": "gbar_Im", + "value": 0.001208331925254469, + "mechanism": "Im" + }, + { + "section": "soma", + "name": "gbar_Ih", + "value": 3.3895743062339889e-07, + "mechanism": "Ih" + }, + { + "section": "soma", + "name": "gbar_NaTs", + "value": 0.37190842732044982, + "mechanism": "NaTs" + }, + { + "section": "soma", + "name": "gbar_Nap", + "value": 0.0039571777539675118, + "mechanism": "Nap" + }, + { + "section": "soma", + "name": "gbar_K_P", + "value": 0.16775882342830961, + "mechanism": "K_P" + }, + { + "section": "soma", + "name": "gbar_K_T", + "value": 0.0049255740705275164, + "mechanism": "K_T" + }, + { + "section": "soma", + "name": "gbar_SK", + "value": 0.0021013149742564741, + "mechanism": "SK" + }, + { + "section": "soma", + "name": "gbar_Kv3_1", + "value": 0.14828964332694683, + "mechanism": "Kv3_1" + }, + { + "section": "soma", + "name": "gbar_Ca_HVA", + "value": 0.00042916703400973334, + "mechanism": "Ca_HVA" + }, + { + "section": "soma", + "name": "gbar_Ca_LVA", + "value": 0.00082631165050270241, + "mechanism": "Ca_LVA" + }, + { + "section": "soma", + "name": "gamma_CaDynamics", + "value": 0.0011356235818674677, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "decay_CaDynamics", + "value": 805.79133357008755, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "g_pas", + "value": 0.00023989400647704806, + "mechanism": "" + }, + { + "section": "axon", + "name": "g_pas", + "value": 0.00016046138825921941, + "mechanism": "" + }, + { + "section": "dend", + "name": "g_pas", + "value": 0.00021576881414849498, + "mechanism": "" + }, + { + "section": "apic", + "name": "g_pas", + "value": 9.7428255654937774e-05, + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Pvalb_473862421_params.json b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Pvalb_473862421_params.json new file mode 100644 index 00000000..97acb020 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Pvalb_473862421_params.json @@ -0,0 +1,135 @@ +{ + "passive": [ + { + "ra": 165.043810596, + "cm": [ + { + "section": "soma", + "cm": 2.22229833871 + }, + { + "section": "axon", + "cm": 2.22229833871 + }, + { + "section": "dend", + "cm": 2.22229833871 + } + ], + "e_pas": -87.37230682373047 + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 40 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -87.37230682373047 + } + ], + "genome": [ + { + "section": "soma", + "name": "gbar_Ih", + "value": 0.00028918148171832306, + "mechanism": "Ih" + }, + { + "section": "soma", + "name": "gbar_NaV", + "value": 0.058014543022859681, + "mechanism": "NaV" + }, + { + "section": "soma", + "name": "gbar_Kd", + "value": 0.0010690585854891154, + "mechanism": "Kd" + }, + { + "section": "soma", + "name": "gbar_Kv2like", + "value": 0.0019155385688926097, + "mechanism": "Kv2like" + }, + { + "section": "soma", + "name": "gbar_Kv3_1", + "value": 1.1418319671301926, + "mechanism": "Kv3_1" + }, + { + "section": "soma", + "name": "gbar_K_T", + "value": 0.02809468489424316, + "mechanism": "K_T" + }, + { + "section": "soma", + "name": "gbar_Im_v2", + "value": 0.0063814397958922133, + "mechanism": "Im_v2" + }, + { + "section": "soma", + "name": "gbar_SK", + "value": 8.8148305240377811e-06, + "mechanism": "SK" + }, + { + "section": "soma", + "name": "gbar_Ca_HVA", + "value": 0.00073439372435641964, + "mechanism": "Ca_HVA" + }, + { + "section": "soma", + "name": "gbar_Ca_LVA", + "value": 0.00021377849056254395, + "mechanism": "Ca_LVA" + }, + { + "section": "soma", + "name": "gamma_CaDynamics", + "value": 0.0043320648574568773, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "decay_CaDynamics", + "value": 569.01425505518728, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "g_pas", + "value": 0.00057441529168598753, + "mechanism": "" + }, + { + "section": "axon", + "name": "g_pas", + "value": 9.5525621425433387e-05, + "mechanism": "" + }, + { + "section": "dend", + "name": "g_pas", + "value": 1.0002747846356705e-07, + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Rorb_486509958_params.json b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Rorb_486509958_params.json new file mode 100644 index 00000000..e9e87314 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Rorb_486509958_params.json @@ -0,0 +1,145 @@ +{ + "passive": [ + { + "ra": 28.292678117573736, + "cm": [ + { + "section": "soma", + "cm": 1.0 + }, + { + "section": "axon", + "cm": 1.0 + }, + { + "section": "dend", + "cm": 2.523057408207212 + }, + { + "section": "apic", + "cm": 2.523057408207212 + } + ], + "e_pas": -95.52335357666016 + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 49 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -95.52335357666016 + } + ], + "genome": [ + { + "section": "soma", + "name": "gbar_Ih", + "value": 2.5374725765871712e-06, + "mechanism": "Ih" + }, + { + "section": "soma", + "name": "gbar_NaV", + "value": 0.34453074059624594, + "mechanism": "NaV" + }, + { + "section": "soma", + "name": "gbar_Kd", + "value": 2.1562149985856615e-11, + "mechanism": "Kd" + }, + { + "section": "soma", + "name": "gbar_Kv2like", + "value": 0.011400590254160202, + "mechanism": "Kv2like" + }, + { + "section": "soma", + "name": "gbar_Kv3_1", + "value": 0.23775079867328591, + "mechanism": "Kv3_1" + }, + { + "section": "soma", + "name": "gbar_K_T", + "value": 0.021795766278746794, + "mechanism": "K_T" + }, + { + "section": "soma", + "name": "gbar_Im_v2", + "value": 0.011617082423130925, + "mechanism": "Im_v2" + }, + { + "section": "soma", + "name": "gbar_SK", + "value": 0.00083538434923959015, + "mechanism": "SK" + }, + { + "section": "soma", + "name": "gbar_Ca_HVA", + "value": 0.00038419005745996053, + "mechanism": "Ca_HVA" + }, + { + "section": "soma", + "name": "gbar_Ca_LVA", + "value": 0.00020569925173446801, + "mechanism": "Ca_LVA" + }, + { + "section": "soma", + "name": "gamma_CaDynamics", + "value": 0.0053165424812619414, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "decay_CaDynamics", + "value": 952.33389461845229, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "g_pas", + "value": 0.00040582050830603744, + "mechanism": "" + }, + { + "section": "axon", + "name": "g_pas", + "value": 0.00054376303566492973, + "mechanism": "" + }, + { + "section": "dend", + "name": "g_pas", + "value": 6.9660029616666359e-05, + "mechanism": "" + }, + { + "section": "apic", + "name": "g_pas", + "value": 4.6275302055329323e-05, + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Scnn1a_485510712_params.json b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Scnn1a_485510712_params.json new file mode 100644 index 00000000..09ae90db --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/biophysical_neuron_models/Scnn1a_485510712_params.json @@ -0,0 +1,145 @@ +{ + "passive": [ + { + "ra": 97.884995612, + "cm": [ + { + "section": "soma", + "cm": 1.0 + }, + { + "section": "axon", + "cm": 1.0 + }, + { + "section": "dend", + "cm": 6.60030189809047 + }, + { + "section": "apic", + "cm": 6.60030189809047 + } + ], + "e_pas": -87.3881607055664 + } + ], + "fitting": [ + { + "junction_potential": -14.0, + "sweeps": [ + 35 + ] + } + ], + "conditions": [ + { + "celsius": 34.0, + "erev": [ + { + "ena": 53.0, + "section": "soma", + "ek": -107.0 + } + ], + "v_init": -87.3881607055664 + } + ], + "genome": [ + { + "section": "soma", + "name": "gbar_Im", + "value": 3.6252897138377983e-06, + "mechanism": "Im" + }, + { + "section": "soma", + "name": "gbar_Ih", + "value": 6.8401950770180524e-13, + "mechanism": "Ih" + }, + { + "section": "soma", + "name": "gbar_NaTs", + "value": 0.80445811847908832, + "mechanism": "NaTs" + }, + { + "section": "soma", + "name": "gbar_Nap", + "value": 1.5287439466549202e-07, + "mechanism": "Nap" + }, + { + "section": "soma", + "name": "gbar_K_P", + "value": 0.086345660923300316, + "mechanism": "K_P" + }, + { + "section": "soma", + "name": "gbar_K_T", + "value": 1.7440121102829664e-07, + "mechanism": "K_T" + }, + { + "section": "soma", + "name": "gbar_SK", + "value": 0.0041070704344665643, + "mechanism": "SK" + }, + { + "section": "soma", + "name": "gbar_Kv3_1", + "value": 0.093344807514917139, + "mechanism": "Kv3_1" + }, + { + "section": "soma", + "name": "gbar_Ca_HVA", + "value": 0.00060833855377752605, + "mechanism": "Ca_HVA" + }, + { + "section": "soma", + "name": "gbar_Ca_LVA", + "value": 0.0091769239503974153, + "mechanism": "Ca_LVA" + }, + { + "section": "soma", + "name": "gamma_CaDynamics", + "value": 0.00065480591105915873, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "decay_CaDynamics", + "value": 312.55384609975539, + "mechanism": "CaDynamics" + }, + { + "section": "soma", + "name": "g_pas", + "value": 9.5062399071850021e-05, + "mechanism": "" + }, + { + "section": "axon", + "name": "g_pas", + "value": 6.7437165553712972e-06, + "mechanism": "" + }, + { + "section": "dend", + "name": "g_pas", + "value": 1.8372762835837482e-06, + "mechanism": "" + }, + { + "section": "apic", + "name": "g_pas", + "value": 0.00040237231931351884, + "mechanism": "" + } + ] +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/electrodes/linear_electrode.csv b/docs/tutorial/05_opt_extracellular/components/electrodes/linear_electrode.csv new file mode 100644 index 00000000..919496bc --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/electrodes/linear_electrode.csv @@ -0,0 +1,10 @@ +channel x_pos y_pos z_pos +0 0.0 0.0 0.0 +1 0.0 -100.0 0.0 +2 0.0 -200.0 0.0 +3 0.0 -300.0 0.0 +4 0.0 -400.0 0.0 +5 0.0 -500.0 0.0 +6 0.0 -600.0 0.0 +7 0.0 -700.0 0.0 +8 0.0 -800.0 0.0 \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/filter_models/tOFF_TF15.json b/docs/tutorial/05_opt_extracellular/components/filter_models/tOFF_TF15.json new file mode 100644 index 00000000..4e9b43af --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/filter_models/tOFF_TF15.json @@ -0,0 +1,51 @@ +{ + "sopt_msg": [ + "After ", + "8", + "function evaluations, TNC returned:", + "Converged (|f_n-f_(n-1)| ~= 0)" + ], + "sopt_nf": 8, + "sopt_res": [ + 3.4416603571978417, + -2.1155994819051305, + 8.269733598229024, + 19.99148791096526 + ], + "init_prms": [ + 3.44215357, + -2.11509939, + 8.27421573, + 20.0 + ], + "opt_wts": [ + 3.4416603571978417, + -2.1155994819051305 + ], + "prms_bds": [ + [ + 1.0, + 10.0 + ], + [ + -10.0, + -0.05 + ], + [ + 2, + 100 + ], + [ + 2, + 200 + ] + ], + "opt_delays": [ + 0, + 0 + ], + "opt_kpeaks": [ + 8.269733598229024, + 19.99148791096526 + ] +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/filter_models/tON_TF8.json b/docs/tutorial/05_opt_extracellular/components/filter_models/tON_TF8.json new file mode 100644 index 00000000..e6116ab8 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/filter_models/tON_TF8.json @@ -0,0 +1,51 @@ +{ + "sopt_msg": [ + "After ", + "20", + "function evaluations, TNC returned:", + "Converged (|f_n-f_(n-1)| ~= 0)" + ], + "sopt_nf": 20, + "sopt_res": [ + 4.21848979193672, + -2.404989833331929, + 8.524645232832142, + 23.246816713624384 + ], + "init_prms": [ + 4.222, + -2.404, + 8.545, + 23.019 + ], + "opt_wts": [ + 4.21848979193672, + -2.404989833331929 + ], + "prms_bds": [ + [ + 1.0, + 10.0 + ], + [ + -10.0, + -0.05 + ], + [ + 2, + 100 + ], + [ + 2, + 200 + ] + ], + "opt_delays": [ + 0, + 0 + ], + "opt_kpeaks": [ + 8.524645232832142, + 23.246816713624384 + ] +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/CaDynamics.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/CaDynamics.mod new file mode 100644 index 00000000..12af0659 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/CaDynamics.mod @@ -0,0 +1,40 @@ +: Dynamics that track inside calcium concentration +: modified from Destexhe et al. 1994 + +NEURON { + SUFFIX CaDynamics + USEION ca READ ica WRITE cai + RANGE decay, gamma, minCai, depth +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + FARADAY = (faraday) (coulombs) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) +} + +PARAMETER { + gamma = 0.05 : percent of free calcium (not buffered) + decay = 80 (ms) : rate of removal of calcium + depth = 0.1 (um) : depth of shell + minCai = 1e-4 (mM) +} + +ASSIGNED {ica (mA/cm2)} + +INITIAL { + cai = minCai +} + +STATE { + cai (mM) +} + +BREAKPOINT { SOLVE states METHOD cnexp } + +DERIVATIVE states { + cai' = -(10000)*(ica*gamma/(2*FARADAY*depth)) - (cai - minCai)/decay +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ca_HVA.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ca_HVA.mod new file mode 100644 index 00000000..84db2d3c --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ca_HVA.mod @@ -0,0 +1,82 @@ +: Reference: Reuveni, Friedman, Amitai, and Gutnick, J.Neurosci. 1993 + +NEURON { + SUFFIX Ca_HVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + UNITSOFF + : if((v == -27) ){ + : v = v+0.0001 + : } + :mAlpha = (0.055*(-27-v))/(exp((-27-v)/3.8) - 1) + mAlpha = 0.055 * vtrap(-27 - v, 3.8) + mBeta = (0.94*exp((-75-v)/17)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + hAlpha = (0.000457*exp((-13-v)/50)) + hBeta = (0.0065/(exp((-v-15)/28)+1)) + hInf = hAlpha/(hAlpha + hBeta) + hTau = 1/(hAlpha + hBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ca_LVA.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ca_LVA.mod new file mode 100644 index 00000000..ab151d0e --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ca_LVA.mod @@ -0,0 +1,69 @@ +: Comment: LVA ca channel. Note: mtau is an approximation from the plots +: Reference: Avery and Johnston 1996, tau from Randall 1997 +: Comment: shifted by -10 mv to correct for junction potential +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Ca_LVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1.0000/(1+ exp((v - -30.000)/-6)) + mTau = (5.0000 + 20.0000/(1+exp((v - -25.000)/5)))/qt + hInf = 1.0000/(1+ exp((v - -80.000)/6.4)) + hTau = (20.0000 + 50.0000/(1+exp((v - -40.000)/7)))/qt + v = v - 10 + UNITSON +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ih.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ih.mod new file mode 100644 index 00000000..73b97d84 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Ih.mod @@ -0,0 +1,71 @@ +: Reference: Kole,Hallermann,and Stuart, J. Neurosci. 2006 + +NEURON { + SUFFIX Ih + NONSPECIFIC_CURRENT ihcn + RANGE gbar, g, ihcn +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + ehcn = -45.0 (mV) +} + +ASSIGNED { + v (mV) + ihcn (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ihcn = g*(v-ehcn) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + : if(v == -154.9){ + : v = v + 0.0001 + : } + :mAlpha = 0.001*6.43*(v+154.9)/(exp((v+154.9)/11.9)-1) + mAlpha = 0.001 * 6.43 * vtrap(v + 154.9, 11.9) + mBeta = 0.001*193*exp(v/33.1) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Im.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Im.mod new file mode 100644 index 00000000..d6112d57 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Im.mod @@ -0,0 +1,62 @@ +: Reference: Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Im + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Im_v2.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Im_v2.mod new file mode 100644 index 00000000..fc219f71 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Im_v2.mod @@ -0,0 +1,59 @@ +: Based on Im model of Vervaeke et al. (2006) + +NEURON { + SUFFIX Im_v2 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-30)/10) + mAlpha = 0.007 * exp( (6 * 0.4 * (v - (-48))) / 26.12 ) + mBeta = 0.007 * exp( (-6 * (1 - 0.4) * (v - (-48))) / 26.12 ) + + mInf = mAlpha / (mAlpha + mBeta) + mTau = (15 + 1 / (mAlpha + mBeta)) / qt +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/K_P.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/K_P.mod new file mode 100644 index 00000000..0a1238f9 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/K_P.mod @@ -0,0 +1,71 @@ +: Comment: The persistent component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + + +NEURON { + SUFFIX K_P + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + tauF = 1 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mInf = 1 / (1 + exp(-(v - (-14.3 + vshift)) / 14.6)) + if (v < -50 + vshift){ + mTau = tauF * (1.25+175.03*exp(-(v - vshift) * -0.026))/qt + } else { + mTau = tauF * (1.25+13*exp(-(v - vshift) * 0.026))/qt + } + hInf = 1/(1 + exp(-(v - (-54 + vshift))/-11)) + hTau = (360+(1010+24*(v - (-55 + vshift)))*exp(-((v - (-75 + vshift))/48)^2))/qt + UNITSON +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/K_T.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/K_T.mod new file mode 100644 index 00000000..c31beaff --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/K_T.mod @@ -0,0 +1,68 @@ +: Comment: The transient component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + +NEURON { + SUFFIX K_T + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + mTauF = 1.0 + hTauF = 1.0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1/(1 + exp(-(v - (-47 + vshift)) / 29)) + mTau = (0.34 + mTauF * 0.92*exp(-((v+71-vshift)/59)^2))/qt + hInf = 1/(1 + exp(-(v+66-vshift)/-10)) + hTau = (8 + hTauF * 49*exp(-((v+73-vshift)/23)^2))/qt + UNITSON +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kd.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kd.mod new file mode 100644 index 00000000..82cbe59a --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kd.mod @@ -0,0 +1,62 @@ +: Based on Kd model of Foust et al. (2011) + + +NEURON { + SUFFIX Kd + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * h + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h' = (hInf - h) / hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-23)/10) + mInf = 1 - 1 / (1 + exp((v - (-43)) / 8)) + mTau = 1 + hInf = 1 / (1 + exp((v - (-67)) / 7.3)) + hTau = 1500 +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kv2like.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kv2like.mod new file mode 100644 index 00000000..1cbdf843 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kv2like.mod @@ -0,0 +1,86 @@ +: Kv2-like channel +: Adapted from model implemented in Keren et al. 2005 +: Adjusted parameters to be similar to guangxitoxin-sensitive current in mouse CA1 pyramids from Liu and Bean 2014 + + +NEURON { + SUFFIX Kv2like + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mAlpha + mBeta + mTau + hInf + h1Tau + h2Tau +} + +STATE { + m + h1 + h2 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * m * (0.5 * h1 + 0.5 * h2) + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h1' = (hInf - h1) / h1Tau + h2' = (hInf - h2) / h2Tau +} + +INITIAL{ + rates() + m = mInf + h1 = hInf + h2 = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mAlpha = 0.12 * vtrap( -(v - 43), 11.0) + mBeta = 0.02 * exp(-(v + 1.27) / 120) + mInf = mAlpha / (mAlpha + mBeta) + mTau = 2.5 * (1 / (qt * (mAlpha + mBeta))) + + hInf = 1/(1 + exp((v + 58) / 11)) + h1Tau = (360 + (1010 + 23.7 * (v + 54)) * exp(-((v + 75) / 48)^2)) / qt + h2Tau = (2350 + 1380 * exp(-0.011 * v) - 210 * exp(-0.03 * v)) / qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kv3_1.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kv3_1.mod new file mode 100644 index 00000000..e2446577 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Kv3_1.mod @@ -0,0 +1,54 @@ +: Comment: Kv3-like potassium current + +NEURON { + SUFFIX Kv3_1 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + mInf = 1/(1+exp(((v -(18.700 + vshift))/(-9.700)))) + mTau = 0.2*20.000/(1+exp(((v -(-46.560 + vshift))/(-44.140)))) + UNITSON +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaTa.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaTa.mod new file mode 100644 index 00000000..fcf7bd39 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaTa.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTa + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -48 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -69 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaTs.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaTs.mod new file mode 100644 index 00000000..f753e718 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaTs.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTs + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -40 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -66 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaV.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaV.mod new file mode 100644 index 00000000..a7023956 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/NaV.mod @@ -0,0 +1,186 @@ +TITLE Mouse sodium current +: Kinetics of Carter et al. (2012) +: Based on 37 degC recordings from mouse hippocampal CA1 pyramids + +NEURON { + SUFFIX NaV + USEION na READ ena WRITE ina + RANGE g, gbar +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) +} + +PARAMETER { + gbar = .015 (S/cm2) + + : kinetic parameters + Con = 0.01 (/ms) : closed -> inactivated transitions + Coff = 40 (/ms) : inactivated -> closed transitions + Oon = 8 (/ms) : open -> Ineg transition + Ooff = 0.05 (/ms) : Ineg -> open transition + alpha = 400 (/ms) + beta = 12 (/ms) + gamma = 250 (/ms) : opening + delta = 60 (/ms) : closing + + alfac = 2.51 + btfac = 5.32 + + : Vdep + x1 = 24 (mV) : Vdep of activation (alpha) + x2 = -24 (mV) : Vdep of deactivation (beta) +} + +ASSIGNED { + + : rates + f01 (/ms) + f02 (/ms) + f03 (/ms) + f04 (/ms) + f0O (/ms) + f11 (/ms) + f12 (/ms) + f13 (/ms) + f14 (/ms) + f1n (/ms) + fi1 (/ms) + fi2 (/ms) + fi3 (/ms) + fi4 (/ms) + fi5 (/ms) + fin (/ms) + + b01 (/ms) + b02 (/ms) + b03 (/ms) + b04 (/ms) + b0O (/ms) + b11 (/ms) + b12 (/ms) + b13 (/ms) + b14 (/ms) + b1n (/ms) + bi1 (/ms) + bi2 (/ms) + bi3 (/ms) + bi4 (/ms) + bi5 (/ms) + bin (/ms) + + v (mV) + ena (mV) + ina (milliamp/cm2) + g (S/cm2) + celsius (degC) +} + +STATE { + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + C5 FROM 0 TO 1 + I1 FROM 0 TO 1 + I2 FROM 0 TO 1 + I3 FROM 0 TO 1 + I4 FROM 0 TO 1 + I5 FROM 0 TO 1 + O FROM 0 TO 1 + I6 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * O + ina = g * (v - ena) +} + +INITIAL { + rates(v) + SOLVE seqinitial +} + +KINETIC activation +{ + rates(v) + ~ C1 <-> C2 (f01,b01) + ~ C2 <-> C3 (f02,b02) + ~ C3 <-> C4 (f03,b03) + ~ C4 <-> C5 (f04,b04) + ~ C5 <-> O (f0O,b0O) + ~ O <-> I6 (fin,bin) + ~ I1 <-> I2 (f11,b11) + ~ I2 <-> I3 (f12,b12) + ~ I3 <-> I4 (f13,b13) + ~ I4 <-> I5 (f14,b14) + ~ I5 <-> I6 (f1n,b1n) + ~ C1 <-> I1 (fi1,bi1) + ~ C2 <-> I2 (fi2,bi2) + ~ C3 <-> I3 (fi3,bi3) + ~ C4 <-> I4 (fi4,bi4) + ~ C5 <-> I5 (fi5,bi5) + + CONSERVE C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +LINEAR seqinitial { : sets initial equilibrium + ~ I1*bi1 + C2*b01 - C1*( fi1+f01) = 0 + ~ C1*f01 + I2*bi2 + C3*b02 - C2*(b01+fi2+f02) = 0 + ~ C2*f02 + I3*bi3 + C4*b03 - C3*(b02+fi3+f03) = 0 + ~ C3*f03 + I4*bi4 + C5*b04 - C4*(b03+fi4+f04) = 0 + ~ C4*f04 + I5*bi5 + O*b0O - C5*(b04+fi5+f0O) = 0 + ~ C5*f0O + I6*bin - O*(b0O+fin) = 0 + + ~ C1*fi1 + I2*b11 - I1*( bi1+f11) = 0 + ~ I1*f11 + C2*fi2 + I3*b12 - I2*(b11+bi2+f12) = 0 + ~ I2*f12 + C3*fi3 + I4*bi3 - I3*(b12+bi3+f13) = 0 + ~ I3*f13 + C4*fi4 + I5*b14 - I4*(b13+bi4+f14) = 0 + ~ I4*f14 + C5*fi5 + I6*b1n - I5*(b14+bi5+f1n) = 0 + + ~ C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +PROCEDURE rates(v(mV) ) +{ + LOCAL qt + qt = 2.3^((celsius-37)/10) + + f01 = qt * 4 * alpha * exp(v/x1) + f02 = qt * 3 * alpha * exp(v/x1) + f03 = qt * 2 * alpha * exp(v/x1) + f04 = qt * 1 * alpha * exp(v/x1) + f0O = qt * gamma + f11 = qt * 4 * alpha * alfac * exp(v/x1) + f12 = qt * 3 * alpha * alfac * exp(v/x1) + f13 = qt * 2 * alpha * alfac * exp(v/x1) + f14 = qt * 1 * alpha * alfac * exp(v/x1) + f1n = qt * gamma + fi1 = qt * Con + fi2 = qt * Con * alfac + fi3 = qt * Con * alfac^2 + fi4 = qt * Con * alfac^3 + fi5 = qt * Con * alfac^4 + fin = qt * Oon + + b01 = qt * 1 * beta * exp(v/x2) + b02 = qt * 2 * beta * exp(v/x2) + b03 = qt * 3 * beta * exp(v/x2) + b04 = qt * 4 * beta * exp(v/x2) + b0O = qt * delta + b11 = qt * 1 * beta * exp(v/x2) / btfac + b12 = qt * 2 * beta * exp(v/x2) / btfac + b13 = qt * 3 * beta * exp(v/x2) / btfac + b14 = qt * 4 * beta * exp(v/x2) / btfac + b1n = qt * delta + bi1 = qt * Coff + bi2 = qt * Coff / (btfac) + bi3 = qt * Coff / (btfac^2) + bi4 = qt * Coff / (btfac^3) + bi5 = qt * Coff / (btfac^4) + bin = qt * Ooff +} + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Nap.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Nap.mod new file mode 100644 index 00000000..ef8021ec --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/Nap.mod @@ -0,0 +1,77 @@ +:Reference : Modeled according to kinetics derived from Magistretti & Alonso 1999 +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Nap + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + hInf + hTau + hAlpha + hBeta +} + +STATE { + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + rates() + g = gbar*mInf*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1.0/(1+exp((v- -52.6)/-4.6)) : assuming instantaneous activation as modeled by Magistretti and Alonso + + hInf = 1.0/(1+exp((v- -48.8)/10)) + hAlpha = 2.88e-6 * vtrap(v + 17, 4.63) + hBeta = 6.94e-6 * vtrap(-(v + 64.4), 2.63) + + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/SK.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/SK.mod new file mode 100644 index 00000000..8bfa3b72 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/SK.mod @@ -0,0 +1,56 @@ +: SK-type calcium-activated potassium current +: Reference : Kohler et al. 1996 + +NEURON { + SUFFIX SK + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gbar, g, ik +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (milli/liter) +} + +PARAMETER { + v (mV) + gbar = .000001 (mho/cm2) + zTau = 1 (ms) + ek (mV) + cai (mM) +} + +ASSIGNED { + zInf + ik (mA/cm2) + g (S/cm2) +} + +STATE { + z FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * z + ik = g * (v - ek) +} + +DERIVATIVE states { + rates(cai) + z' = (zInf - z) / zTau +} + +PROCEDURE rates(ca(mM)) { + if(ca < 1e-7){ + ca = ca + 1e-07 + } + zInf = 1/(1 + (0.00043 / ca)^4.8) +} + +INITIAL { + rates(cai) + z = zInf +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/exp1isyn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/exp1isyn.mod new file mode 100644 index 00000000..e8700401 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/exp1isyn.mod @@ -0,0 +1,52 @@ +COMMENT +Current-based version of the conductance-based model exp1syn (refer to the exp1syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + +NEURON { + POINT_PROCESS exp1isyn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*e +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/exp1syn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/exp1syn.mod new file mode 100644 index 00000000..6763e687 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/exp1syn.mod @@ -0,0 +1,42 @@ +NEURON { + POINT_PROCESS exp1syn + RANGE tau, e, i + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (uS) = (microsiemens) +} + +PARAMETER { + tau = 0.1 (ms) <1e-9,1e9> + e = 0 (mV) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + g (uS) +} + +INITIAL { + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v-e) +} + +DERIVATIVE state { + g' = -g/tau +} + +NET_RECEIVE(weight (uS)) { + g = g + weight +} diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp1syn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp1syn.mod new file mode 100644 index 00000000..3490c587 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp1syn.mod @@ -0,0 +1,75 @@ +COMMENT +A model (# 1 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp1syn + RANGE e, i, tau_r, p0, tau_1 + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + g +} + +INITIAL { + n=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + + +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp2syn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp2syn.mod new file mode 100644 index 00000000..ce1d1625 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp2syn.mod @@ -0,0 +1,95 @@ +COMMENT +A model (# 2 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp2syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) + (uS) = (microsiemens) + +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_1 = baseline level of release probability + tau_1 = 5 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + tau_r + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p0 +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + tau_r' = (tau_r0-tau_r)/tau_FDR +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp3syn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp3syn.mod new file mode 100644 index 00000000..31869840 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp3syn.mod @@ -0,0 +1,102 @@ +COMMENT +A model (# 3 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,5,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp3syn + RANGE e, i, p0, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : p0 = baseline level of release probability + p0 = 0.3 + + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = magnitude of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D + a_D = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + tau_r + D + g +} + +INITIAL { + n=1 + tau_r=tau_r0 + g=0 + D=1 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0*D + n = n - n*p0 + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p0*n*D + +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp4syn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp4syn.mod new file mode 100644 index 00000000..1ba964be --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp4syn.mod @@ -0,0 +1,83 @@ +COMMENT +A model (# 4 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by +solving Eqs (3,4) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." + +State variables: + + n = fraction of available vesicles + p = release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where +w is the static synaptic weight and Pmax is the dynamically changing factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1 + +Implemented by Sergey L. Gratiy + +ENDCOMMENT + + + + +NEURON { + POINT_PROCESS stp4syn + RANGE e, i, tau_r, p0, tau_1,tau_f + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + : tau_r = recovery time constant + tau_r = 100 (ms) + : p0 = baseline level of release probability + p0 = 0.3 + : tau_f = facilitation time constant + tau_f = 1000 (ms) < 0, 1e9 > + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) +} + +ASSIGNED { + v (mV) + i (nA) +} + +STATE { + n + p + g +} + +INITIAL { + n=1 + p=1 + g=0 +} + +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) +} + +DERIVATIVE state { + n' = (1-n)/tau_r + g' = -g/tau_1 + p' = 0 +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*n*p0 + n = n - n*p0 + p = p + p0*(1-p) + +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp5isyn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp5isyn.mod new file mode 100644 index 00000000..d6b6c105 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp5isyn.mod @@ -0,0 +1,113 @@ +COMMENT +Current-based version of the conductance-based model stp5syn (refer to the stp5syn.mod) +In this model the synatic current is defined as i = g*e, whereas in the conductance based model i = g*(v - e). +Since the current is proportional to conductance, this model may be used to report the values of conductance by recording the current. + +ENDCOMMENT + + +NEURON { + POINT_PROCESS stp5isyn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of pb + pbtilde = 0.5 + +} + +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +STATE { + n + p + tau_r + D + pb + g +} + +INITIAL { + n=1 + pb=pbtilde + p=pb + tau_r=tau_r0 + D=1 + g=0 + +} + +BREAKPOINT { + SOLVE state METHOD cnexp +: i = g*(v - e) + i = g*e + Pmax = n*p*D + +} + +DERIVATIVE state { + g' = -g/tau_1 + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + +} + +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp5syn.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp5syn.mod new file mode 100644 index 00000000..621fe9e1 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/stp5syn.mod @@ -0,0 +1,131 @@ +COMMENT +A model (# 5 using terminology of Jung Hoon Lee) of a short-term synaptic plasticity. +The magnitude of the peak conductance is found by solving Eqs (3,4,5,8,10) in Hennig, 2013. "Theoretical models of synaptic short term plasticity. Frontiers in computational neuroscience, 7, p.45." +This file could be used to simulate simpler models by setting to zero the parameters in the unused equations(e.g, a_D,a_i,a_f). + +State variables: + + n = fraction of available vesicles + D = fraction of non-desensitized receptors + tau_r = time constant for vesicle replenishment + pb = baseline vesicle release probability + p = vesicle release probability + +After each spike the magnitude of the peak conductance changes by the factor w*Pmax, where w is the static synaptic weight and Pmax is the activity-dependent factor that could be interpreted as a probability of a transmitter release by the presynaptic terminal. + +The post_synaptic dynamics of individual synaptic events is modeled by a single exponential synapse with the time constant tau_1. + +Implemented by Sergey L. Gratiy, + +ENDCOMMENT + +: Declaring parameters as RANGE allows them to change as a function of a position alogn the cell +NEURON { + POINT_PROCESS stp5syn + RANGE e, i, tau_1, tau_r0, a_FDR, tau_FDR, a_D, tau_D, a_i, tau_i, a_f, tau_f, pbtilde, Pmax + NONSPECIFIC_CURRENT i +} + +UNITS { + (nA) = (nanoamp) + (mV) = (millivolt) + (umho) = (micromho) +} + +: Declaration of the default values of parameters +PARAMETER { + : e = synaptic reversal potential + e = 0 (mV) + + : tau_1 = baseline level of release probability + tau_1 = 10 (ms) + + : tau_r0 = baseline level of tau_r0 + tau_r0 = 1000 (ms) + + : tau_FDR = time constant for tau_r relaxation + tau_FDR = 1000 (ms) + + : a_FDR = amount of tau_r reduction after each spike + a_FDR = 0.5 + + : tau_D = relaxation time of D + tau_D = 100 (ms) + + : a_D = amount of desentization + a_D = 0.5 + + : tau_i = relaxation time for p0 + tau_i = 100 (ms) + + : a_i = amount of decrease of baseline probability after each spike + a_i = 0.5 + + : tau_f = facilitation time constant (relaxation time constant for p) + tau_f = 10 (ms) + + : a_f = amount of facilitation (increase of p after each spike) + a_f = 0.5 + + : pbtilde = baseline level of p0 + pbtilde = 0.5 + +} +: Declaration of dependent and external variables that collectively are called ASSIGNED +ASSIGNED { + v (mV) + i (nA) + Pmax +} + +: Declaration of the state variables +STATE { + n + p + tau_r + D + pb + g +} + +: Initial conditions for the state variables +INITIAL { + n=1 + p=1 + tau_r=tau_r0 + D=1 + pb=pbtilde + g=0 + +} + +: Integration method + assignment statements +BREAKPOINT { + SOLVE state METHOD cnexp + i = g*(v - e) + Pmax = n*p*D +} + +: Definition of teh dynamics between the presynaptic activations +DERIVATIVE state { + n' = (1-n)/tau_r + p' = (pb-p)/tau_f + tau_r' = (tau_r0-tau_r)/tau_FDR + D' = (1-D)/tau_D + pb' = (pbtilde-pb)/tau_i + g' = -g/tau_1 + +} + +: This block defines what happens to the state variables at the moment presynaptic activation +NET_RECEIVE(weight (umho)) { + g = g + weight*Pmax + n = n - n*p + p = p + a_f*(1-p) + tau_r = tau_r - a_FDR*tau_r + D = D - a_D*p*n*D + pb = pb - a_i*pb + +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/vecevent.mod b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/vecevent.mod new file mode 100644 index 00000000..503dfd2e --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/mechanisms/modfiles/vecevent.mod @@ -0,0 +1,71 @@ +: Vector stream of events + +NEURON { + ARTIFICIAL_CELL VecStim +} + +ASSIGNED { + index + etime (ms) + space +} + +INITIAL { + index = 0 + element() + if (index > 0) { + net_send(etime - t, 1) + } +} + +NET_RECEIVE (w) { + if (flag == 1) { + net_event(t) + element() + if (index > 0) { + net_send(etime - t, 1) + } + } +} + +VERBATIM +extern double* vector_vec(); +extern int vector_capacity(); +extern void* vector_arg(); +ENDVERBATIM + +PROCEDURE element() { +VERBATIM + { void* vv; int i, size; double* px; + i = (int)index; + if (i >= 0) { + vv = *((void**)(&space)); + if (vv) { + size = vector_capacity(vv); + px = vector_vec(vv); + if (i < size) { + etime = px[i]; + index += 1.; + }else{ + index = -1.; + } + }else{ + index = -1.; + } + } + } +ENDVERBATIM +} + +PROCEDURE play() { +VERBATIM + void** vv; + vv = (void**)(&space); + *vv = (void*)0; + if (ifarg(1)) { + *vv = vector_arg(1); + } +ENDVERBATIM +} + + diff --git a/docs/tutorial/05_opt_extracellular/components/morphologies/Nr5a1_485507735_morphology.swc b/docs/tutorial/05_opt_extracellular/components/morphologies/Nr5a1_485507735_morphology.swc new file mode 100644 index 00000000..0db07da9 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/morphologies/Nr5a1_485507735_morphology.swc @@ -0,0 +1,1793 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): 485307311/Nr5a1-Cre_Ai14-199361.04.02.01_485307311_RD_longeraxon.swc_sorted.swc_Z_T10.swc_sorted.swc.out.swc +# id,type,x,y,z,r,pid +1 1 405.0904 453.7104 27.44 4.0515 -1 +2 3 408.1414 451.991 25.7367 0.1144 1 +3 3 409.1047 451.4064 25.7243 0.2822 2 +4 3 409.9009 450.6159 25.682 0.2775 3 +5 3 410.6285 449.8402 25.8139 0.3342 4 +6 3 411.3755 449.0452 26.0763 0.3514 5 +7 3 411.9029 448.2455 26.1364 0.3517 6 +8 3 412.1786 447.1942 26.2849 0.2769 7 +9 3 412.6637 446.3202 26.6423 0.2758 8 +10 3 413.1819 445.302 26.9742 0.2921 9 +11 3 413.699 444.3273 27.2919 0.347 10 +12 3 414.2607 443.4773 27.6683 0.3298 11 +13 3 414.7915 442.4798 27.9294 0.4173 12 +14 3 415.3143 441.6046 28.2097 0.4581 13 +15 3 415.9515 440.7638 28.4936 0.4369 14 +16 3 416.9022 440.1895 28.6751 0.4101 15 +17 3 417.8574 439.6014 28.8408 0.2992 16 +18 3 418.7257 438.867 28.9811 0.277 17 +19 3 419.5609 438.1108 29.1488 0.2599 18 +20 3 420.1672 437.191 29.1967 0.3405 19 +21 3 420.968 436.4085 29.1542 0.4024 20 +22 3 421.7631 435.5917 29.1368 0.37 21 +23 3 422.5696 434.8001 29.1939 0.3381 22 +24 3 423.4768 434.1263 29.3098 0.3709 23 +25 3 424.4126 433.4708 29.4193 0.3285 24 +26 3 425.25 432.7169 29.4669 0.2931 25 +27 3 425.7133 431.6884 29.4896 0.2471 26 +28 3 425.7854 430.5753 29.6198 0.2952 27 +29 3 425.8551 429.4622 29.8301 0.3087 28 +30 3 425.8654 428.3216 30.0412 0.2439 29 +31 3 425.8654 427.1788 30.2408 0.2159 30 +32 3 425.9055 426.0382 30.4254 0.174 31 +33 3 426.1137 424.9216 30.5967 0.163 32 +34 3 426.323 423.8062 30.7353 0.162 33 +35 3 426.3391 423.6186 31.626 0.1144 34 +36 3 426.7875 422.6394 31.9164 0.1413 35 +37 3 427.7347 422.017 32.0152 0.1447 36 +38 3 428.4703 421.1487 32.0902 0.1509 37 +39 3 429.1293 420.2232 32.1423 0.1629 38 +40 3 430.0067 419.5025 32.1734 0.1833 39 +41 3 430.9254 418.8413 32.1852 0.2301 40 +42 3 431.6735 417.9764 32.1793 0.2729 41 +43 3 432.0808 416.9731 32.1709 0.2223 42 +44 3 432.0888 415.8291 32.1591 0.1879 43 +45 3 432.0888 414.6851 32.1429 0.1787 44 +46 3 432.1517 413.5457 32.1205 0.2396 45 +47 3 432.5807 412.5413 32.0894 0.2028 46 +48 3 433.4845 411.8755 32.0429 0.1748 47 +49 3 434.5129 411.3744 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259.8436 329.0613 50.4532 0.2314 1345 +1347 4 258.9204 328.4172 50.6164 0.2181 1346 +1348 4 258.0544 327.6942 50.8273 0.2839 1347 +1349 4 257.2192 326.914 51.0303 0.3107 1348 +1350 4 256.383 326.1407 51.2481 0.2506 1349 +1351 4 255.5421 325.3868 51.4996 0.2136 1350 +1352 4 254.7471 324.5825 51.7432 0.2422 1351 +1353 4 253.9428 323.7737 51.9294 0.2803 1352 +1354 4 253.0551 323.053 52.071 0.3097 1353 +1355 4 252.1788 322.3197 52.1861 0.335 1354 +1356 4 251.3242 321.559 52.283 0.4099 1355 +1357 4 250.4605 320.8096 52.3757 0.4124 1356 +1358 4 249.6654 319.9985 52.5 0.3933 1357 +1359 4 248.8921 319.1829 52.6924 0.3579 1358 +1360 4 247.9826 318.5182 52.9166 0.2921 1359 +1361 4 247.0136 317.9119 53.1188 0.2854 1360 +1362 4 246.087 317.2426 53.2983 0.3997 1361 +1363 4 245.1523 316.5906 53.4534 0.4542 1362 +1364 4 244.1639 316.0231 53.5954 0.5183 1363 +1365 4 243.2018 315.4397 53.804 0.4736 1364 +1366 4 242.3152 314.8128 54.1495 0.3841 1365 +1367 4 241.4881 314.5417 54.6795 0.2499 1366 +1368 4 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184.1794 99.918 0.1374 1609 +1611 4 130.4023 183.1647 100.3397 0.1375 1610 +1612 4 130.9846 182.1797 100.651 0.1376 1611 +1613 4 131.6 181.2153 100.8759 0.1379 1612 +1614 4 131.6996 180.1377 101.1657 0.1384 1613 +1615 4 131.7087 179.0337 101.4927 0.1398 1614 +1616 4 131.6035 177.8954 101.7391 0.1398 1615 +1617 4 131.7362 176.7583 101.9211 0.1525 1616 +1618 4 132.1251 175.6829 102.1174 0.1144 1617 +1619 4 134.5012 225.1312 82.3385 0.1144 1569 +1620 4 134.1477 224.2778 80.7937 0.1385 1619 +1621 4 134.0459 223.5136 80.0402 0.1395 1620 +1622 4 133.3309 222.6968 79.2932 0.1414 1621 +1623 4 132.8115 221.7907 78.7562 0.1449 1622 +1624 4 132.5358 220.9007 78.3289 0.1513 1623 +1625 4 132.4088 219.9077 77.7815 0.1637 1624 +1626 4 131.8849 218.9959 77.275 0.1839 1625 +1627 4 131.1985 218.1025 76.823 0.235 1626 +1628 4 130.527 217.2204 76.3619 0.2639 1627 +1629 4 130.1575 216.2377 75.8486 0.2939 1628 +1630 4 129.5283 215.3008 75.4684 0.2351 1629 +1631 4 129.2022 214.405 75.3502 0.2234 1630 +1632 4 128.7572 213.7861 75.448 0.1874 1631 +1633 4 128.843 212.9911 75.3572 0.1897 1632 +1634 4 129.4894 212.3413 75.0019 0.2028 1633 +1635 4 129.4333 211.2739 74.6035 0.2977 1634 +1636 4 128.8396 210.3187 74.1499 0.246 1635 +1637 4 127.9621 209.6357 73.6361 0.2248 1636 +1638 4 127.2711 208.7949 73.0747 0.1669 1637 +1639 4 126.7255 207.8751 72.5292 0.15 1638 +1640 4 126.15 206.9244 72.0888 0.1372 1639 +1641 4 125.2966 206.1866 71.776 0.1372 1640 +1642 4 124.299 205.6294 71.5728 0.1371 1641 +1643 4 123.4067 204.9362 71.4585 0.137 1642 +1644 4 122.8564 203.9615 71.4106 0.1368 1643 +1645 4 122.6528 202.8449 71.4 0.1364 1644 +1646 4 122.082 201.9286 71.4 0.1356 1645 +1647 4 121.0318 201.6094 71.4 0.1342 1646 +1648 4 120.2447 200.8772 71.4 0.1313 1647 +1649 4 120.2344 199.7367 71.4 0.1271 1648 +1650 4 120.2344 198.5927 71.4 0.1144 1649 +1651 4 120.12 197.4544 71.4 0.1144 1650 +1652 4 383.9092 439.5671 31.9519 0.1144 1197 +1653 4 383.3498 440.4606 33.3855 0.1569 1652 +1654 4 382.8213 441.3689 34.004 0.1715 1653 +1655 4 382.8213 442.4294 34.7152 0.2108 1654 +1656 4 382.819 443.491 35.4687 0.2244 1655 +1657 4 382.5467 444.5778 36.1533 0.1946 1656 +1658 4 382.2367 445.6578 36.7472 0.1776 1657 +1659 4 381.421 446.454 37.182 0.1892 1658 +1660 4 381.0161 447.5156 37.4923 0.2299 1659 +1661 4 381.1739 448.6471 37.7314 0.3265 1660 +1662 4 381.3547 449.735 38.015 0.4031 1661 +1663 4 381.0184 450.8046 38.3303 0.3559 1662 +1664 4 380.9223 451.9406 38.6036 0.387 1663 +1665 4 380.6294 453.0263 38.8508 0.4402 1664 +1666 4 380.4166 454.0799 39.2218 0.4469 1665 +1667 4 380.11 455.1633 39.6192 0.4475 1666 +1668 4 379.8172 456.2364 40.0523 0.3915 1667 +1669 4 379.7954 457.3563 40.4933 0.3361 1668 +1670 4 379.6982 458.4637 40.9209 0.2267 1669 +1671 4 379.5803 459.5242 41.3896 0.1714 1670 +1672 4 379.6101 460.6167 41.8636 0.1532 1671 +1673 4 379.5323 461.7333 42.2083 0.168 1672 +1674 4 379.4774 462.8624 42.4768 0.1889 1673 +1675 4 379.4248 463.9789 42.7983 0.2584 1674 +1676 4 379.2795 465.0909 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429.2723 9.0313 0.2853 1785 +1787 3 386.632 428.4108 8.5105 0.2683 1786 +1788 3 385.997 427.4728 8.0296 0.2754 1787 +1789 3 385.4902 426.4649 7.5885 0.2161 1788 +1790 3 384.7466 425.6424 6.734 0.1144 1789 diff --git a/docs/tutorial/05_opt_extracellular/components/morphologies/Pvalb_473862421_morphology.swc b/docs/tutorial/05_opt_extracellular/components/morphologies/Pvalb_473862421_morphology.swc new file mode 100644 index 00000000..67f4709e --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/morphologies/Pvalb_473862421_morphology.swc @@ -0,0 +1,1239 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): C:/Users/alexh/Desktop/Pvalb-IRES-Cre_Ai14_IVSCC_-169125.03.01.01_469628681_p.swc_Z_T10.swc_pruned.swc +# id,type,x,y,z,r,pid +1 1 312.0832 372.8296 27.44 5.1972 -1 +2 3 310.5926 376.7913 27.4061 0.2161 1 +3 3 310.5274 377.8643 27.154 0.2796 2 +4 3 310.6829 378.9637 27.0068 0.2924 3 +5 3 310.6372 380.0368 26.7489 0.2542 4 +6 3 310.1784 380.9817 26.6762 0.2161 5 +7 3 309.7906 381.945 26.7835 0.1907 6 +8 3 309.6373 383.0558 26.7836 0.1907 7 +9 3 308.9521 383.9264 26.6633 0.2288 8 +10 3 308.0803 384.6437 26.4746 0.2796 9 +11 3 307.3013 385.4811 26.2807 0.3686 10 +12 3 306.6515 386.418 26.0403 0.4576 11 +13 3 306.163 386.8493 25.7348 0.2542 12 +14 3 305.2078 387.4522 25.4678 0.2415 13 +15 3 304.1713 387.935 25.2622 0.2415 14 +16 3 303.1646 388.4772 25.0959 0.2034 15 +17 3 302.6612 389.4794 24.9498 0.2415 16 +18 3 302.0526 390.4449 24.811 0.2669 17 +19 3 301.1488 391.113 24.62 0.3051 18 +20 3 300.2908 391.8269 24.3497 0.2669 19 +21 3 299.3196 392.4252 24.0991 0.2542 20 +22 3 298.2831 392.9057 23.8647 0.2542 21 +23 3 297.186 392.9846 23.5735 0.3178 22 +24 3 296.0592 393.1093 23.3124 0.3178 23 +25 3 295.0021 393.4719 23.063 0.3051 24 +26 3 293.9371 393.8781 22.9508 0.2415 25 +27 3 292.9361 394.3848 22.9953 0.2161 26 +28 3 292.4899 394.9992 23.0872 0.2161 27 +29 3 291.9008 395.9762 23.2314 0.2034 28 +30 3 291.2498 396.8708 23.4707 0.2288 29 +31 3 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a/docs/tutorial/05_opt_extracellular/components/morphologies/Rorb_486509958_morphology.swc b/docs/tutorial/05_opt_extracellular/components/morphologies/Rorb_486509958_morphology.swc new file mode 100644 index 00000000..71b60652 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/morphologies/Rorb_486509958_morphology.swc @@ -0,0 +1,2375 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): M:/BatchProcessing/496744050 - GW/reconstructions-496744050/496744050/mylinker.ano.swc_sorted.swc +# id,type,x,y,z,r,pid +1 1 538.7096 613.4128 17.36 5.4782 -1 +2 3 538.2165 608.0932 16.358 0.572 1 +3 3 538.0564 606.9664 16.1925 0.4322 2 +4 3 537.7612 605.8613 16.0682 0.4496 3 +5 3 537.5656 604.7459 15.9826 0.4818 4 +6 3 537.5656 603.6019 15.9233 0.4272 5 +7 3 537.5656 602.4579 15.8788 0.3093 6 +8 3 537.5656 601.3139 15.8391 0.2859 7 +9 3 537.4729 600.179 15.7967 0.3245 8 +10 3 537.6491 599.1608 15.7246 0.341 9 +11 3 538.6524 598.8611 15.6127 0.2962 10 +12 3 539.3296 598.1312 15.4741 0.2353 11 +13 3 539.396 597.0033 15.3653 0.2441 12 +14 3 539.5962 595.8799 15.2956 0.2416 13 +15 3 539.6248 594.7393 15.2636 0.3279 14 +16 3 539.6248 593.5953 15.2681 0.2758 15 +17 3 539.6248 592.4513 15.3073 0.2934 16 +18 3 539.8364 591.3336 15.3934 0.2291 17 +19 3 539.9611 590.2022 15.5142 0.2368 18 +20 3 539.801 589.0719 15.6237 0.2075 19 +21 3 539.6122 587.9519 15.7612 0.2507 20 +22 3 539.007 587.2324 16.7034 0.2288 21 +23 3 538.4614 586.3458 16.2153 0.3023 22 +24 3 537.5359 585.7028 16.0247 0.3394 23 +25 3 536.9284 584.7762 15.8192 0.3301 24 +26 3 536.552 583.71 15.596 0.348 25 +27 3 535.9034 582.8257 15.4109 0.3257 26 +28 3 535.1197 581.9997 15.2862 0.3242 27 +29 3 534.296 581.2115 15.2173 0.3567 28 +30 3 533.2985 580.6658 15.1854 0.3608 29 +31 3 532.2792 580.1476 15.1812 0.412 30 +32 3 531.4097 579.412 15.1944 0.4095 31 +33 3 530.5231 578.6993 15.2523 0.3061 32 +34 3 530.0907 578.101 15.2964 0.251 33 +35 3 529.354 577.2281 15.3273 0.2857 34 +36 3 528.5543 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168.0296 427.8331 38.197 0.1371 1133 +1135 4 168.3888 426.7612 38.3225 0.137 1134 +1136 4 168.7 425.6847 38.5081 0.1368 1135 +1137 4 168.7446 424.5487 38.6641 0.1364 1136 +1138 4 168.7503 423.4047 38.7769 0.1356 1137 +1139 4 168.9425 422.279 38.8525 0.1342 1138 +1140 4 169.1759 421.1602 38.8982 0.1313 1139 +1141 4 169.4115 420.0402 38.9178 0.1271 1140 +1142 4 169.4996 418.8996 38.92 0.1144 1141 +1143 4 169.7696 417.7888 38.92 0.1144 1142 +1144 4 223.9197 487.4298 46.0043 0.3325 995 +1145 4 223.1143 488.051 46.1457 0.331 1144 +1146 4 222.6361 487.0157 46.2921 0.3979 1145 +1147 4 222.1511 485.9918 46.4069 0.4128 1146 +1148 4 221.3583 485.1784 46.4951 0.3978 1147 +1149 4 220.4419 484.5012 46.5612 0.3483 1148 +1150 4 219.5325 483.8342 46.6239 0.3617 1149 +1151 4 218.4834 483.388 46.7009 0.3336 1150 +1152 4 217.4275 482.9499 46.7634 0.4243 1151 +1153 4 216.3968 482.4614 46.839 0.471 1152 +1154 4 215.4232 481.8722 46.9174 0.4607 1153 +1155 4 214.357 481.6251 46.9753 0.455 1154 +1156 4 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193.6712 467.0998 48.3423 0.4633 1177 +1179 4 192.907 466.2658 48.4221 0.4727 1178 +1180 4 192.0284 465.5325 48.5192 0.4638 1179 +1181 4 191.3283 464.6539 48.6696 0.4601 1180 +1182 4 190.6339 463.7616 48.8846 0.5061 1181 +1183 4 189.6283 463.455 49.191 0.4485 1182 +1184 4 188.5827 463.0592 49.5242 0.4643 1183 +1185 4 187.5474 462.573 49.8274 0.4696 1184 +1186 4 186.8152 462.0719 50.297 0.3676 1185 +1187 4 185.6712 462.057 50.6724 0.2636 1186 +1188 4 184.5341 461.9918 50.9967 0.2301 1187 +1189 4 183.4107 461.8637 51.2744 0.309 1188 +1190 4 182.3444 461.4564 51.483 0.3431 1189 +1191 4 181.2908 461.0412 51.6286 0.3785 1190 +1192 4 180.204 460.7449 51.7429 0.4655 1191 +1193 4 179.4192 460.0218 51.8949 0.5227 1192 +1194 4 178.8244 459.0678 52.0758 0.562 1193 +1195 4 178.2306 458.1079 52.2735 0.501 1194 +1196 4 177.4424 457.33 52.4776 0.4663 1195 +1197 4 176.4837 456.71 52.675 0.4791 1196 +1198 4 175.7962 455.836 52.9054 0.4718 1197 +1199 4 175.0446 455.01 53.0359 0.4939 1198 +1200 4 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1264 +1266 4 158.3262 476.8284 79.8148 0.3418 1265 +1267 4 158.7174 477.5033 80.2816 0.2415 1266 +1268 4 158.5504 478.2126 80.8786 0.178 1267 +1269 4 158.7506 479.2182 81.5128 0.1525 1268 +1270 4 158.706 480.3564 82.6 0.2288 1269 +1271 4 515.5093 605.9539 19.7755 0.572 707 +1272 4 516.5389 606.4195 20.1218 0.4981 1271 +1273 4 517.5307 606.9686 20.2665 0.3704 1272 +1274 4 518.5615 607.4457 20.3891 0.2827 1273 +1275 4 519.5785 607.9536 20.5055 0.3117 1274 +1276 4 520.3999 608.7258 20.6244 0.3513 1275 +1277 4 520.9444 609.7188 20.7302 0.264 1276 +1278 4 521.8276 610.0403 20.7776 0.1957 1277 +1279 4 522.6787 609.3116 20.8482 0.1876 1278 +1280 4 523.5493 608.6023 20.9983 0.1686 1279 +1281 4 524.5091 608.0452 21.2104 0.1531 1280 +1282 4 525.5971 607.7454 21.3861 0.1431 1281 +1283 4 526.7388 607.7592 21.5338 0.1481 1282 +1284 4 527.8759 607.8861 21.6649 0.1571 1283 +1285 4 529.0073 608.0394 21.7837 0.1749 1284 +1286 4 530.133 608.0326 21.8814 0.2029 1285 +1287 4 531.2084 607.9822 21.9818 0.2792 1286 +1288 4 532.2311 608.457 22.1291 0.3019 1287 +1289 4 533.3053 608.7178 22.2492 0.2345 1288 +1290 4 534.4356 608.576 22.3005 0.1833 1289 +1291 4 535.5636 608.4558 22.3049 0.1882 1290 +1292 4 536.7053 608.5016 22.3077 0.1697 1291 +1293 4 537.8459 608.5977 22.3181 0.1552 1292 +1294 4 538.9876 608.6297 22.3436 0.1469 1293 +1295 4 540.127 608.6618 22.3951 0.1552 1294 +1296 4 541.2539 608.8128 22.501 0.17 1295 +1297 4 542.3212 609.1182 22.6895 0.2001 1296 +1298 4 543.2982 609.6719 22.9197 0.2437 1297 +1299 4 544.3301 609.935 23.1125 0.3833 1298 +1300 4 545.4684 609.8504 23.2395 0.3568 1299 +1301 4 546.6078 609.9076 23.33 0.3204 1300 +1302 4 547.7449 610.0311 23.3929 0.3049 1301 +1303 4 548.882 610.1501 23.4323 0.2529 1302 +1304 4 550.0226 610.2165 23.456 0.2691 1303 +1305 4 551.1495 610.3778 23.4732 0.2104 1304 +1306 4 552.2729 610.578 23.475 0.1888 1305 +1307 4 553.4054 610.7267 23.4629 0.1834 1306 +1308 4 554.5448 610.753 23.46 0.2341 1307 +1309 4 555.682 610.6432 23.4797 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26.3354 0.2683 1330 +1332 4 580.2986 609.9087 26.4974 0.3164 1331 +1333 4 581.2984 610.4476 26.6489 0.3212 1332 +1334 4 582.4161 610.6283 26.7697 0.2825 1333 +1335 4 583.5212 610.3549 26.8618 0.2126 1334 +1336 4 584.5565 609.8721 26.9321 0.1885 1335 +1337 4 585.6559 609.5701 26.9991 0.2037 1336 +1338 4 586.7931 609.466 27.0573 0.2844 1337 +1339 4 587.9325 609.434 27.0907 0.2937 1338 +1340 4 589.0273 609.1537 27.1323 0.3062 1339 +1341 4 590.0386 608.6515 27.2054 0.2373 1340 +1342 4 591.1654 608.4536 27.2822 0.213 1341 +1343 4 592.2957 608.2808 27.3659 0.2386 1342 +1344 4 593.434 608.1756 27.4651 0.2861 1343 +1345 4 594.5665 608.1046 27.6136 0.2597 1344 +1346 4 595.6751 608.0589 27.8443 0.1941 1345 +1347 4 596.8111 608.1596 28.0742 0.1531 1346 +1348 4 597.9459 608.2065 28.2926 0.1431 1347 +1349 4 599.0842 608.1069 28.4777 0.148 1348 +1350 4 600.2259 608.139 28.6336 0.1576 1349 +1351 4 601.339 608.3517 28.7624 0.1728 1350 +1352 4 602.4144 608.7247 28.856 0.2133 1351 +1353 4 603.5058 609.0107 28.9363 0.2288 1352 +1354 4 604.429 609.6662 29.0265 0.2038 1353 +1355 4 605.1474 610.5448 29.1194 0.1896 1354 +1356 4 605.6988 611.5469 29.2029 0.2363 1355 +1357 4 606.4722 612.2642 29.2849 0.1968 1356 +1358 4 607.5612 612.278 29.3924 0.1631 1357 +1359 4 608.632 611.8936 29.528 0.138 1358 +1360 4 609.7245 611.5893 29.6666 0.1386 1359 +1361 4 610.6866 611.9439 29.8164 0.1397 1360 +1362 4 611.587 612.6383 29.9762 0.1419 1361 +1363 4 612.6623 612.8591 30.1412 0.1457 1362 +1364 4 613.8029 612.906 30.3122 0.1533 1363 +1365 4 614.7627 613.4425 30.4758 0.1645 1364 +1366 4 615.6962 614.0248 30.7216 0.2003 1365 +1367 4 616.7762 614.3246 30.9708 0.1931 1366 +1368 4 617.8778 614.6277 31.18 0.1933 1367 +1369 4 618.9715 614.9 31.4222 0.2431 1368 +1370 4 620.0583 614.6609 31.6546 0.2094 1369 +1371 4 621.1405 614.2937 31.8604 0.1874 1370 +1372 4 622.1896 613.8773 32.0788 0.1779 1371 +1373 4 623.2844 613.565 32.2714 0.2381 1372 +1374 4 624.3998 613.7205 32.44 0.2002 1373 +1375 4 625.4374 613.3247 32.5724 0.1695 1374 +1376 4 626.5585 613.1142 32.662 0.1497 1375 +1377 4 627.6991 613.0364 32.7169 0.161 1376 +1378 4 628.8408 612.9644 32.7438 0.178 1377 +1379 4 629.9505 613.2355 32.7561 0.2288 1378 +1380 4 631.0716 613.4643 32.76 0.2288 1379 +1381 4 521.9592 610.1192 20.5659 0.3432 1278 +1382 4 522.8549 610.7519 21.4498 0.2497 1381 +1383 4 523.5848 611.5973 21.7937 0.211 1382 +1384 4 524.4439 612.2185 22.2085 0.2411 1383 +1385 4 525.3603 612.7344 22.7028 0.2603 1384 +1386 4 525.771 613.7983 23.127 0.3596 1385 +1387 4 526.5489 614.495 23.4942 0.3486 1386 +1388 4 527.3108 615.0396 23.7833 0.3604 1387 +1389 4 527.948 615.79 24.071 0.3404 1388 +1390 4 528.9547 616.0955 24.4378 0.2771 1389 +1391 4 529.1378 617.0473 24.7694 0.2877 1390 +1392 4 530.2188 617.0999 25.066 0.2583 1391 +1393 4 531.0265 617.8767 25.3723 0.2125 1392 +1394 4 532.0092 618.4292 25.688 0.2 1393 +1395 4 532.9267 619.0802 26.0122 0.1681 1394 +1396 4 533.8453 619.7323 26.335 0.1521 1395 +1397 4 534.5226 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537.7372 11.0912 0.1701 1856 +1858 3 566.5305 536.7488 10.9913 0.1873 1857 +1859 3 566.4184 535.6357 10.8503 0.1681 1858 +1860 3 566.717 534.558 10.6955 0.1521 1859 +1861 3 567.4869 533.9002 10.4342 0.1412 1860 +1862 3 567.9148 532.9782 10.1668 0.1441 1861 +1863 3 567.9377 531.8433 9.997 0.1525 1862 +1864 3 567.8896 530.7188 9.955 0.1525 1863 +1865 3 568.2248 529.672 9.8 0.2288 1864 +1866 3 558.7868 595.6282 17.3552 0.1968 1799 +1867 3 559.5979 594.8365 17.4938 0.163 1866 +1868 3 560.4101 594.046 17.6763 0.1379 1867 +1869 3 560.1253 594.2988 16.604 0.2288 1868 +1870 3 560.0978 595.3159 15.7636 0.3461 1869 +1871 3 560.7533 594.8434 15.4774 0.2699 1870 +1872 3 561.5164 594.0575 15.085 0.2461 1871 +1873 3 562.1833 593.1629 14.623 0.2039 1872 +1874 3 563.1512 592.6217 14.1362 0.2308 1873 +1875 3 563.3422 593.4752 13.5801 0.2272 1874 +1876 3 563.0356 594.4293 13.0481 0.2511 1875 +1877 3 563.6614 594.2497 12.4327 0.3766 1876 +1878 3 564.6052 593.7383 11.8517 0.3291 1877 +1879 3 564.7756 594.4579 11.3936 0.3432 1878 +1880 3 564.9587 595.5756 11.0169 0.3305 1879 +1881 3 565.3465 596.6486 10.734 0.2669 1880 +1882 3 565.2515 597.7377 10.08 0.3432 1881 +1883 3 560.5978 593.7371 17.8123 0.1384 1868 +1884 3 561.1446 592.751 17.9535 0.1393 1883 +1885 3 561.99 592.0589 18.1054 0.141 1884 +1886 3 563.102 591.9765 18.2342 0.1442 1885 +1887 3 563.6511 591.1117 18.344 0.1501 1886 +1888 3 564.6395 590.6632 18.4487 0.1608 1887 +1889 3 565.462 589.9013 18.5986 0.1818 1888 +1890 3 566.4482 589.3293 18.7457 0.2155 1889 +1891 3 567.5384 589.2836 19.0079 0.3037 1890 +1892 3 568.6435 589.5146 19.2426 0.342 1891 +1893 3 569.5324 590.2113 19.454 0.3343 1892 +1894 3 570.6466 590.3955 19.6819 0.2443 1893 +1895 3 571.7678 590.2056 19.9149 0.2169 1894 +1896 3 572.8637 589.9013 20.1533 0.1756 1895 +1897 3 573.8739 589.4654 20.3799 0.1667 1896 +1898 3 574.6804 588.6841 20.5908 0.1654 1897 +1899 3 575.6665 588.1212 20.8169 0.2028 1898 +1900 3 576.6835 587.7025 21.033 0.1941 1899 +1901 3 577.8184 587.6705 21.2458 0.213 1900 +1902 3 578.9258 587.5229 21.4815 0.1932 1901 +1903 3 580.0023 587.1614 21.7152 0.1934 1902 +1904 3 581.0937 587.1202 21.9436 0.2431 1903 +1905 3 582.2262 587.2129 22.1273 0.21 1904 +1906 3 583.3542 587.063 22.2574 0.185 1905 +1907 3 584.4902 586.9795 22.3398 0.1914 1906 +1908 3 585.5575 586.6409 22.3847 0.1755 1907 +1909 3 586.6775 586.5334 22.4067 0.1665 1908 +1910 3 587.7369 586.1456 22.4157 0.1651 1909 +1911 3 588.7665 585.6479 22.4218 0.2022 1910 +1912 3 589.8922 585.5141 22.4313 0.1931 1911 +1913 3 591.0362 585.4969 22.447 0.2112 1912 +1914 3 592.1802 585.4878 22.471 0.1892 1913 +1915 3 593.1148 584.886 22.469 0.1891 1914 +1916 3 594.0609 584.2465 22.4903 0.2205 1915 +1917 3 595.15 584.0532 22.6192 0.2397 1916 +1918 3 596.2757 584.2408 22.7502 0.2356 1917 +1919 3 597.4185 584.2397 22.8929 0.1923 1918 +1920 3 598.5545 584.1928 23.0682 0.1708 1919 +1921 3 599.6608 583.9525 23.2742 0.1886 1920 +1922 3 600.7384 583.6871 23.5353 0.1704 1921 +1923 3 601.8287 583.3508 23.7343 0.1564 1922 +1924 3 602.9052 583.1334 23.8965 0.1497 1923 +1925 3 603.9451 583.1883 24.1386 0.1576 1924 +1926 3 604.5125 582.4584 24.4352 0.1877 1925 +1927 3 605.2252 582.2159 24.6644 0.1688 1926 +1928 3 606.2925 582.6255 24.8403 0.1534 1927 +1929 3 607.4194 582.7525 24.9826 0.1435 1928 +1930 3 608.5416 582.5694 25.0945 0.1494 1929 +1931 3 609.6387 582.2491 25.1861 0.1571 1930 +1932 3 610.7507 582.0889 25.3305 0.1868 1931 +1933 3 611.8901 582.0672 25.4839 0.1671 1932 +1934 3 613.0296 582.153 25.6265 0.1503 1933 +1935 3 614.1324 581.9654 25.8187 0.1379 1934 +1936 3 615.1608 581.4998 26.0382 0.1383 1935 +1937 3 616.2785 581.263 26.2114 0.1393 1936 +1938 3 617.3951 581.057 26.3799 0.1409 1937 +1939 3 618.4144 580.54 26.5097 0.1441 1938 +1940 3 619.349 579.8913 26.6034 0.1497 1939 +1941 3 620.1887 579.2106 26.6451 0.1615 1940 +1942 3 621.3247 579.15 26.6969 0.1768 1941 +1943 3 622.4321 578.896 26.8119 0.2362 1942 +1944 3 623.4697 578.4911 26.9533 0.1966 1943 +1945 3 624.2842 577.696 27.1139 0.1628 1944 +1946 3 625.1399 577.0107 27.3115 0.1374 1945 +1947 3 625.8424 576.2202 27.5355 0.1376 1946 +1948 3 626.3835 575.2158 27.7467 0.1379 1947 +1949 3 627.0367 574.2811 27.9433 0.1384 1948 +1950 3 627.6396 573.311 28.1355 0.1393 1949 +1951 3 627.9759 572.2288 28.3237 0.141 1950 +1952 3 628.2242 571.1123 28.5242 0.1441 1951 +1953 3 628.493 570.0117 28.7927 0.15 1952 +1954 3 628.771 568.9181 29.1424 0.1607 1953 +1955 3 629.2869 567.9159 29.5302 0.1817 1954 +1956 3 629.9905 567.7844 30.032 0.2147 1955 +1957 3 630.0569 568.886 30.6054 0.3051 1956 +1958 3 630.4538 569.855 31.2511 0.3304 1957 +1959 3 631.4605 570.149 31.8578 0.3818 1958 +1960 3 632.3231 570.6672 32.5354 0.3408 1959 +1961 3 633.2109 571.3273 33.206 0.2403 1960 +1962 3 633.633 570.7107 33.8778 0.1729 1961 +1963 3 633.0885 569.807 34.5646 0.1566 1962 +1964 3 633.1926 569.1377 35.2122 0.1709 1963 +1965 3 634.2519 568.735 35.6941 0.2098 1964 +1966 3 635.2163 568.6458 36.0626 0.2225 1965 +1967 3 636.0343 568.4422 36.3118 0.1915 1966 +1968 3 636.2207 567.3416 36.4994 0.1693 1967 +1969 3 636.4404 566.399 36.8595 0.1858 1968 +1970 3 637.4254 565.8373 37.1829 0.1652 1969 +1971 3 638.2673 565.1703 37.5936 0.1469 1970 +1972 3 639.2008 564.5091 37.9081 0.1313 1971 +1973 3 640.1847 563.9268 38.134 0.1271 1972 +1974 3 641.1697 563.3445 38.2746 0.1144 1973 +1975 3 642.1547 562.7622 38.36 0.1144 1974 +1976 2 543.5133 616.0108 15.7826 0.4576 1 +1977 2 544.5028 616.5737 15.6293 0.3915 1976 +1978 2 545.4786 617.1182 15.5966 0.2818 1977 +1979 2 546.3378 617.8618 15.5744 0.2754 1978 +1980 2 547.1763 618.5688 15.5013 0.2227 1979 +1981 2 548.262 618.8056 15.4736 0.2089 1980 +1982 2 549.3339 618.5311 15.3882 0.2338 1981 +1983 2 550.1565 617.7749 15.2456 0.264 1982 +1984 2 550.7742 616.8128 15.1023 0.2823 1983 +1985 2 551.6265 616.2842 14.9437 0.2709 1984 +1986 2 552.6939 616.5714 14.7812 0.3551 1985 +1987 2 553.6846 617.1114 14.6957 0.3432 1986 +1988 2 554.6798 617.5976 14.6111 0.3356 1987 +1989 2 555.7392 617.9488 14.4588 0.3656 1988 +1990 2 556.7917 618.3675 14.2981 0.3219 1989 +1991 2 557.8007 618.904 14.1644 0.2657 1990 +1992 2 558.7982 619.4646 14.0578 0.2696 1991 +1993 2 559.7638 620.0766 13.9692 0.3241 1992 +1994 2 560.7396 620.6726 13.9045 0.31 1993 +1995 2 561.7177 621.2652 13.8583 0.3857 1994 +1996 2 562.6878 621.8647 13.786 0.374 1995 +1997 2 563.5916 622.5522 13.6807 0.4046 1996 +1998 2 564.5674 623.1322 13.5813 0.4363 1997 +1999 2 565.541 623.7294 13.5022 0.3878 1998 +2000 2 566.391 624.4879 13.4399 0.475 1999 +2001 2 567.4068 624.9695 13.3913 0.4638 2000 +2002 2 568.4364 625.4626 13.3537 0.4811 2001 +2003 2 569.3391 626.157 13.3205 0.4431 2002 +2004 2 570.3606 626.65 13.2812 0.3698 2003 +2005 2 571.4509 626.9257 13.1748 0.2474 2004 +2006 2 572.5114 627.3364 13.0772 0.1835 2005 +2007 2 573.4483 627.9851 13.0038 0.1887 2006 +2008 2 574.304 628.7435 12.9521 0.1706 2007 +2009 2 575.2673 629.3567 12.9179 0.1567 2008 +2010 2 576.3186 629.796 12.92 0.1502 2009 +2011 2 577.3962 630.1781 12.9413 0.1587 2010 +2012 2 578.2806 630.8908 12.9488 0.1897 2011 +2013 2 579.2266 631.5246 12.9121 0.1723 2012 +2014 2 580.2734 631.9616 12.822 0.161 2013 +2015 2 581.2733 632.5165 12.7431 0.1525 2014 +2016 2 582.2502 633.1113 12.6787 0.1907 2015 +2017 2 583.138 633.8321 12.6 0.1144 2016 +2018 3 541.9746 617.9442 18.8819 0.6864 1 +2019 3 542.3841 618.9704 19.1748 0.58 2018 +2020 3 542.6587 620.0743 19.4218 0.4611 2019 +2021 3 543.1415 620.9678 19.3989 0.4048 2020 +2022 3 544.0132 621.6919 19.3144 0.3047 2021 +2023 3 545.0771 621.7045 18.5861 0.4576 2022 +2024 3 545.4546 621.2801 18.6936 0.3432 2023 +2025 3 546.1628 620.3832 18.9626 0.2694 2024 +2026 3 547.1798 620.7447 19.1184 0.2765 2025 +2027 3 548.0183 621.5123 19.2905 0.2217 2026 +2028 3 548.9381 622.1083 19.4267 0.2221 2027 +2029 3 550.0306 622.3051 19.5305 0.1852 2028 +2030 3 550.9687 622.9606 19.6056 0.1849 2029 +2031 3 551.8427 623.6939 19.6548 0.1972 2030 +2032 3 552.5875 624.5588 19.6836 0.2718 2031 +2033 3 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338.2511 609.0828 39.0858 0.1356 720 +722 3 337.5795 608.6309 40.4418 0.1342 721 +723 3 337.0773 608.0223 41.9219 0.1313 722 +724 3 337.0178 607.4617 43.528 0.1271 723 +725 3 336.7192 606.8371 45.1116 0.1144 724 +726 3 336.2216 606.0912 48.16 0.1144 725 +727 3 344.7673 610.6672 32.5923 0.1373 714 +728 3 344.1861 610.9315 31.9721 0.1373 727 +729 3 343.1874 611.3216 31.439 0.1373 728 +730 3 342.3466 611.595 31.2875 0.1373 729 +731 3 341.5755 612.3477 31.376 0.1373 730 +732 3 340.9555 613.2847 31.6165 0.1373 731 +733 3 340.3789 614.2376 32.0001 0.1373 732 +734 3 339.8732 615.2592 32.3823 0.1373 733 +735 3 339.2841 616.2282 32.7076 0.1373 734 +736 3 338.5954 617.1194 33.0086 0.1373 735 +737 3 338.0726 618.094 33.3124 0.1373 736 +738 3 337.7397 619.1774 33.5558 0.1374 737 +739 3 337.7786 620.2837 33.7131 0.1374 738 +740 3 337.9353 621.4139 33.8377 0.1375 739 +741 3 337.8644 622.5362 33.9898 0.1377 740 +742 3 338.012 623.6367 34.0732 0.1381 741 +743 3 338.2957 624.7315 34.1776 0.1389 742 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765 +767 3 365.9061 600.8723 23.7283 0.1878 766 +768 3 365.3707 601.7703 23.6076 0.2015 767 +769 3 364.8937 602.6352 23.5659 0.1708 768 +770 3 364.6626 603.6762 23.6861 0.1572 769 +771 3 364.1752 604.6463 23.7038 0.1503 770 +772 3 363.9236 605.748 23.7118 0.1626 771 +773 3 363.5415 606.8108 23.7548 0.1789 772 +774 3 362.9363 607.7363 23.762 0.2401 773 +775 3 362.1812 608.5943 23.7031 0.2039 774 +776 3 361.5166 609.442 23.493 0.1767 775 +777 3 361.234 610.5334 23.2394 0.1606 776 +778 3 360.9938 611.5973 22.9019 0.1932 777 +779 3 360.8187 612.7092 22.568 0.1796 778 +780 3 360.4195 613.7446 22.3556 0.1708 779 +781 3 359.7033 614.6151 22.248 0.1886 780 +782 3 359.0227 615.5246 22.2222 0.1707 781 +783 3 358.4015 616.465 22.289 0.1567 782 +784 3 357.5229 617.1079 22.2552 0.1503 783 +785 3 356.5962 617.7611 22.1734 0.1588 784 +786 3 355.6707 618.4315 22.0846 0.1899 785 +787 3 354.9008 619.2724 22.0085 0.173 786 +788 3 354.3815 620.286 21.9536 0.161 787 +789 3 354.0989 621.3911 21.925 0.1581 788 +790 3 353.7603 622.4836 21.9357 0.1739 789 +791 3 353.3244 623.5406 21.9696 0.2152 790 +792 3 352.9091 624.6068 22.0183 0.233 791 +793 3 352.4721 625.6628 22.0794 0.2079 792 +794 3 352.0408 626.7187 22.1789 0.2148 793 +795 3 351.5672 627.7265 22.3666 0.1967 794 +796 3 350.906 628.6497 22.5522 0.1999 795 +797 3 350.3225 629.6313 22.6969 0.2551 796 +798 3 349.7849 630.6414 22.791 0.2327 797 +799 3 349.1602 631.5967 22.8505 0.2248 798 +800 3 348.4212 632.4238 22.9383 0.2758 799 +801 3 347.7852 633.3344 22.8723 0.2807 800 +802 3 347.4248 634.3938 22.6865 0.2669 801 +803 3 346.9283 635.4199 22.4545 0.2391 802 +804 3 346.3586 636.3855 22.1397 0.1979 803 +805 3 345.8461 637.4059 21.8217 0.1849 804 +806 3 345.21 638.3554 21.5168 0.1971 805 +807 3 344.4447 639.1722 21.176 0.2717 806 +808 3 343.6553 639.9799 20.8271 0.2727 807 +809 3 342.9129 640.8299 20.4725 0.2545 808 +810 3 342.4438 641.8721 20.1642 0.2037 809 +811 3 341.945 642.8948 19.87 0.1925 810 +812 3 341.2403 643.7883 19.5856 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0.2507 834 +836 3 323.7703 662.4881 9.9085 0.2326 835 +837 3 323.3207 663.5131 9.2421 0.1859 836 +838 3 322.7956 664.5256 8.773 0.1622 837 +839 3 322.076 665.403 8.4632 0.1573 838 +840 3 321.1677 666.0883 8.2666 0.187 839 +841 3 320.217 666.7232 8.1294 0.1676 840 +842 3 319.486 667.5732 8.0078 0.1512 841 +843 3 319.009 668.6074 7.8599 0.1397 842 +844 3 318.2974 669.4482 7.6512 0.1417 843 +845 3 317.3422 669.8566 7.3 0.1455 844 +846 3 316.4293 669.574 6.7928 0.1531 845 +847 3 315.5621 669.9218 6.5817 0.1638 846 +848 3 314.7258 670.6174 6.2733 0.1992 847 +849 3 313.9067 671.2946 6.0861 0.191 848 +850 3 313.75 672.3094 5.7273 0.1894 849 +851 3 313.4091 673.2234 5.312 0.2359 850 +852 3 312.8874 674.2313 4.9583 0.1961 851 +853 3 312.6174 675.0927 4.3767 0.1618 852 +854 3 312.1782 675.9015 3.668 0.1356 853 +855 3 311.1325 676.2973 3.1086 0.1342 854 +856 3 310.0572 676.6874 2.7226 0.1313 855 +857 3 309.3982 677.6084 2.4683 0.1271 856 +858 3 308.6054 678.432 2.3186 0.1144 857 +859 3 308.2851 679.5257 2.2646 0.1144 858 +860 3 384.1415 581.1943 26.9436 0.1144 666 +861 3 383.852 581.7034 28.5093 0.1373 860 +862 3 383.4551 582.439 29.2718 0.1374 861 +863 3 383.3796 583.5201 29.7307 0.1374 862 +864 3 383.2755 584.6561 30.0065 0.1376 863 +865 3 383.1782 585.7692 30.2145 0.1379 864 +866 3 383.1794 586.8743 30.3548 0.1383 865 +867 3 383.6072 587.889 30.259 0.1392 866 +868 3 383.7479 588.6395 29.7867 0.1409 867 +869 3 383.8303 589.4368 29.1236 0.1441 868 +870 3 383.9767 590.4424 28.6205 0.1497 869 +871 3 384.6711 591.1231 28.378 0.1614 870 +872 3 385.6859 591.6436 28.2643 0.1769 871 +873 3 386.5839 592.3392 28.2965 0.2361 872 +874 3 387.1468 593.3059 28.4396 0.1967 873 +875 3 387.4648 594.3892 28.6485 0.1627 874 +876 3 387.5323 595.5138 28.8686 0.1373 875 +877 3 387.5323 596.6406 28.9993 0.1373 876 +878 3 387.6684 597.6576 28.9649 0.1373 877 +879 3 388.205 598.6186 28.943 0.1374 878 +880 3 388.7804 599.5086 29.1444 0.1374 879 +881 3 388.992 600.4021 29.6212 0.1375 880 +882 3 389.3226 601.4054 30.2126 0.1377 881 +883 3 389.7082 602.4521 30.8101 0.1381 882 +884 3 390.1406 603.5103 31.3012 0.1389 883 +885 3 390.5833 604.564 31.6674 0.1403 884 +886 3 391.0444 605.6096 31.9189 0.1425 885 +887 3 391.439 606.6826 32.0597 0.1477 886 +888 3 391.8006 607.766 32.128 0.154 887 +889 3 392.2456 608.8139 32.2263 0.1808 888 +890 3 392.7444 609.8115 32.405 0.1567 889 +891 3 393.3312 610.7908 32.571 0.1271 890 +892 3 393.9456 611.7563 32.8392 0.1144 891 +893 3 393.5749 574.3177 22.1259 0.14 1 +894 3 394.0314 575.3611 21.9994 0.1423 893 +895 3 394.4249 576.2362 22.0301 0.1465 894 +896 3 393.6046 577.0027 21.9459 0.1549 895 +897 3 392.6849 577.5919 21.7517 0.1674 896 +898 3 392.4686 578.4064 21.4082 0.2061 897 +899 3 392.5647 579.3971 21.0385 0.2009 898 +900 3 392.0271 580.2906 20.8499 0.2232 899 +901 3 391.2674 581.0582 20.6664 0.2238 900 +902 3 391.1107 582.0294 20.3739 0.1932 901 +903 3 391.3189 583.0888 19.9794 0.1756 902 +904 3 391.3841 584.2042 19.5854 0.1825 903 +905 3 391.1416 585.3104 19.2392 0.2322 904 +906 3 390.5261 586.1627 18.8071 0.2589 905 +907 3 390.3706 587.2049 18.3604 0.2842 906 +908 3 389.9313 587.5161 18.0901 0.2196 907 +909 3 389.2254 588.3924 17.9411 0.1825 908 +910 3 388.5939 589.3442 17.7947 0.1711 909 +911 3 387.9956 590.2948 17.5785 0.2132 910 +912 3 387.6455 591.3633 17.3166 0.2139 911 +913 3 387.4156 592.4593 16.9957 0.2478 912 +914 3 387.117 593.5507 16.6413 0.2671 913 +915 3 386.7006 594.6146 16.3219 0.285 914 +916 3 386.2876 595.6819 16.0535 0.2911 915 +917 3 385.933 596.7687 15.8219 0.318 916 +918 3 385.7522 597.8899 15.5714 0.2933 917 +919 3 385.5898 598.987 15.2509 0.2666 918 +920 3 385.4708 600.0738 14.8587 0.2382 919 +921 3 385.6481 601.1812 14.463 0.1971 920 +922 3 385.8037 602.3137 14.1567 0.1796 921 +923 3 386.0657 603.4245 13.9539 0.2055 922 +924 3 386.5725 604.4507 13.8133 0.1995 923 +925 3 387.1285 605.4506 13.712 0.2206 924 +926 3 387.6352 606.4653 13.5888 0.2188 925 +927 3 388.0437 607.5029 13.4037 0.1844 926 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949 +951 3 381.1476 631.4251 9.815 0.1436 950 +952 3 380.5493 632.2625 9.5106 0.1494 951 +953 3 380.0036 633.1502 9.3097 0.1572 952 +954 3 379.5803 634.1695 9.0133 0.1868 953 +955 3 379.2577 635.2152 8.6451 0.1673 954 +956 3 379.2154 636.2207 8.1441 0.1506 955 +957 3 379.3207 637.2732 7.6322 0.1383 956 +958 3 379.2005 638.4 7.2719 0.1391 957 +959 3 378.9866 639.5143 7.017 0.1407 958 +960 3 378.8894 640.6103 6.8849 0.1436 959 +961 3 378.8642 641.6993 6.8548 0.1489 960 +962 3 378.6617 642.7781 6.7303 0.1588 961 +963 3 378.1561 643.6762 6.4305 0.1779 962 +964 3 378.0245 644.5102 5.9606 0.2086 963 +965 3 378.4272 645.4986 5.5346 0.2895 964 +966 3 378.8184 646.5545 5.2943 0.3216 965 +967 3 379.2131 647.6115 5.2115 0.2688 966 +968 3 379.6204 648.672 5.2492 0.2569 967 +969 3 379.9544 649.744 5.2866 0.2756 968 +970 3 380.1329 650.8513 5.2633 0.2278 969 +971 3 380.046 651.9748 5.2299 0.1949 970 +972 3 379.8595 653.0375 5.3023 0.2071 971 +973 3 379.7817 654.1335 5.3559 0.2175 972 +974 3 379.6147 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376.1426 590.1244 6.4268 0.2326 1107 +1109 3 376.0889 591.2066 5.7783 0.2175 1108 +1110 3 375.7159 592.2808 5.2625 0.1822 1109 +1111 3 374.938 593.1045 4.9073 0.1525 1110 +1112 3 374.1715 593.8367 4.4891 0.1525 1111 +1113 3 373.7906 594.8136 3.3972 0.1144 1112 +1114 3 382.3843 578.9212 13.288 0.1501 1094 +1115 3 381.4107 579.5104 13.1289 0.1374 1114 +1116 3 380.5974 580.1819 13.1901 0.1375 1115 +1117 3 380.0334 581.0685 13.3631 0.1377 1116 +1118 3 379.3046 581.9185 13.4661 0.1382 1117 +1119 3 378.664 582.8463 13.6246 0.1389 1118 +1120 3 378.1652 583.8541 13.835 0.1403 1119 +1121 3 377.7408 584.8929 13.977 0.1428 1120 +1122 3 377.0944 585.7497 14.0692 0.1475 1121 +1123 3 376.408 586.5425 14.2821 0.1566 1122 +1124 3 375.8578 587.4806 14.5527 0.171 1123 +1125 3 375.3064 588.4679 14.7198 0.21 1124 +1126 3 374.6051 589.0445 14.6119 0.2229 1125 +1127 3 373.635 589.446 14.4297 0.1921 1126 +1128 3 372.8285 590.1015 14.3964 0.1705 1127 +1129 3 372.1478 590.9801 14.4021 0.1881 1128 +1130 3 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3 352.2513 606.1026 11.3507 0.1425 1151 +1153 3 351.224 606.5682 11.0876 0.1469 1152 +1154 3 350.1795 607.0236 10.8699 0.1556 1153 +1155 3 349.1019 607.3313 10.6962 0.1692 1154 +1156 3 347.9693 607.4434 10.5957 0.2065 1155 +1157 3 346.8677 607.7042 10.5656 0.2163 1156 +1158 3 345.7912 608.0829 10.5738 0.1797 1157 +1159 3 344.8153 608.6435 10.5715 0.1501 1158 +1160 3 343.9756 609.4134 10.5115 0.1374 1159 +1161 3 343.1073 610.1501 10.4008 0.1375 1160 +1162 3 342.1395 610.7358 10.2189 0.1376 1161 +1163 3 341.2609 611.4314 9.9919 0.138 1162 +1164 3 340.4567 612.2448 9.7772 0.1384 1163 +1165 3 339.482 612.6246 9.4824 0.1395 1164 +1166 3 338.5153 613.0696 9.1088 0.1414 1165 +1167 3 337.48 613.5078 8.8307 0.1447 1166 +1168 3 336.4836 614.0614 8.6516 0.1517 1167 +1169 3 335.4105 614.4046 8.5127 0.1614 1168 +1170 3 334.4884 615.0361 8.4614 0.1946 1169 +1171 3 333.8112 615.9422 8.4656 0.1821 1170 +1172 3 332.9875 616.7201 8.5473 0.1756 1171 +1173 3 331.9922 617.2441 8.724 0.1975 1172 +1174 3 331.0507 617.8458 8.9918 0.1874 1173 +1175 3 330.2373 618.5837 9.3502 0.1858 1174 +1176 3 329.5235 619.4016 9.7696 0.2138 1175 +1177 3 328.8165 620.286 10.0916 0.2299 1176 +1178 3 328.2319 621.192 10.1847 0.2053 1177 +1179 3 327.7697 622.1861 10.2949 0.1953 1178 +1180 3 326.8946 622.8314 10.4403 0.2323 1179 +1181 3 326.0377 623.5601 10.4468 0.2591 1180 +1182 3 325.349 624.4307 10.4055 0.2845 1181 +1183 3 324.785 625.3916 10.4413 0.2203 1182 +1184 3 324.2382 626.3812 10.425 0.1837 1183 +1185 3 323.863 627.4325 10.2858 0.1734 1184 +1186 3 323.601 628.5273 10.1427 0.2182 1185 +1187 3 323.5232 629.5626 9.9747 0.2204 1186 +1188 3 323.625 630.6197 9.6944 0.272 1187 +1189 3 323.4889 631.7362 9.4578 0.2525 1188 +1190 3 323.212 632.7556 9.3938 0.2042 1189 +1191 3 323.2544 633.8526 9.2944 0.1724 1190 +1192 3 323.3024 634.9852 9.1088 0.1767 1191 +1193 3 322.7945 635.9771 8.8822 0.2211 1192 +1194 3 321.9925 636.731 8.5521 0.2408 1193 +1195 3 321.2524 637.383 8.0384 0.2378 1194 +1196 3 320.4927 637.9516 7.3921 0.1957 1195 +1197 3 319.7983 638.8199 6.9525 0.1809 1196 +1198 3 319.057 639.6722 6.7391 0.189 1197 +1199 3 318.4873 640.6114 6.7475 0.2598 1198 +1200 3 317.9462 641.617 6.8317 0.2352 1199 +1201 3 317.3078 642.5654 6.929 0.2599 1200 +1202 3 316.3789 643.2117 7.0436 0.1935 1201 +1203 3 315.5141 643.953 7.1074 0.1567 1202 +1204 3 314.7853 644.7275 6.9434 0.1271 1203 +1205 3 314.6458 645.8189 6.6622 0.1144 1204 +1206 3 314.9741 646.9137 6.0869 0.1144 1205 +1207 3 389.58 567.8976 17.7072 0.2415 1 +1208 3 388.4555 567.9033 16.78 0.2065 1207 +1209 3 387.3653 568.012 16.4035 0.1814 1208 +1210 3 386.4558 568.1859 15.7378 0.17 1209 +1211 3 385.7007 567.948 14.8109 0.208 1210 +1212 3 384.9938 567.313 13.782 0.2192 1211 +1213 3 383.8864 567.1048 12.8589 0.1849 1212 +1214 3 382.8556 567.5681 12.0635 0.1596 1213 +1215 3 381.7894 567.9491 11.3768 0.1556 1214 +1216 3 380.9749 568.4056 10.6149 0.1692 1215 +1217 3 381.5114 568.1161 9.4984 0.1144 1216 +1218 3 380.8056 567.3908 8.4869 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584.179 25.9239 0.2849 1284 +1286 3 432.6814 585.0794 26.0767 0.275 1285 +1287 3 433.4685 585.8916 26.1004 0.2513 1286 +1288 3 434.2304 586.7118 25.9928 0.2337 1287 +1289 3 434.847 587.6488 25.8712 0.1882 1288 +1290 3 435.4567 588.5788 25.8456 0.1663 1289 +1291 3 436.253 589.3659 25.9293 0.1649 1290 +1292 3 437.159 590.0466 26.0418 0.2018 1291 +1293 3 438.0079 590.7902 26.1314 0.1923 1292 +1294 3 438.7732 591.6379 26.1845 0.2097 1293 +1295 3 439.6438 592.3666 26.2288 0.1864 1294 +1296 3 440.6333 592.9283 26.262 0.184 1295 +1297 3 441.632 593.4809 26.2602 0.2104 1296 +1298 3 442.5598 594.1375 26.2221 0.2234 1297 +1299 3 443.5357 594.713 26.1791 0.1933 1298 +1300 3 444.5573 595.198 26.2035 0.1727 1299 +1301 3 445.3889 595.9279 26.2786 0.1922 1300 +1302 3 445.9255 596.9243 26.3436 0.1771 1301 +1303 3 446.6554 597.7423 26.4352 0.1689 1302 +1304 3 447.6586 598.2617 26.5096 0.1725 1303 +1305 3 448.6459 598.8085 26.4867 0.2009 1304 +1306 3 449.5542 599.4823 26.4393 0.2636 1305 +1307 3 450.4317 600.211 26.3569 0.3308 1306 +1308 3 451.3309 600.8803 26.1779 0.3501 1307 +1309 3 452.2587 601.5335 25.9628 0.3254 1308 +1310 3 453.2539 602.0449 25.8322 0.2302 1309 +1311 3 453.9804 602.856 25.6324 0.1783 1310 +1312 3 454.9528 602.5379 25.3104 0.1637 1311 +1313 3 455.9641 603.0619 25.0274 0.1995 1312 +1314 3 456.8873 603.7243 24.8156 0.188 1313 +1315 3 457.9729 604.0663 24.7047 0.2018 1314 +1316 3 459.0506 604.437 24.634 0.1714 1315 +1317 3 460.1557 604.6326 24.6299 0.1581 1316 +1318 3 461.1727 605.152 24.6135 0.1528 1317 +1319 3 462.2343 605.5581 24.572 0.1632 1318 +1320 3 463.3189 605.8773 24.4304 0.1988 1319 +1321 3 464.3542 606.2411 24.1351 0.1867 1320 +1322 3 465.3964 606.566 23.7283 0.1994 1321 +1323 3 466.434 606.9927 23.3086 0.1669 1322 +1324 3 467.483 607.3519 22.9202 0.15 1323 +1325 3 468.6156 607.4583 22.5744 0.1373 1324 +1326 3 469.747 607.4972 22.2792 0.1373 1325 +1327 3 470.8784 607.4034 22.0361 0.1373 1326 +1328 3 472.0133 607.353 21.8383 0.1373 1327 +1329 3 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34.2672 0.2136 1657 +1659 3 412.4818 568.9192 34.8536 0.2295 1658 +1660 3 413.5732 569.0187 35.434 0.2042 1659 +1661 3 414.7046 569.108 35.9327 0.1939 1660 +1662 3 415.8394 569.2327 36.3513 0.2263 1661 +1663 3 416.8725 569.5999 36.8262 0.2658 1662 +1664 3 417.7556 569.8127 37.4545 0.2094 1663 +1665 3 417.9055 569.9431 37.8952 0.1627 1664 +1666 3 418.7383 570.5872 38.2799 0.1373 1665 +1667 3 419.8549 570.7027 38.5823 0.1373 1666 +1668 3 420.968 570.9224 38.7876 0.1373 1667 +1669 3 422.0353 571.3193 38.9113 0.1374 1668 +1670 3 423.0295 571.8536 39.0709 0.1374 1669 +1671 3 423.9286 572.556 39.258 0.1376 1670 +1672 3 424.559 573.1989 39.6743 0.1379 1671 +1673 3 425.6378 573.4334 40.1097 0.1383 1672 +1674 3 426.7806 573.4586 40.4522 0.1391 1673 +1675 3 427.9201 573.5616 40.6885 0.1408 1674 +1676 3 428.9714 573.9883 40.7991 0.1437 1675 +1677 3 430.0868 574.1713 40.9251 0.1496 1676 +1678 3 431.2182 574.336 40.9931 0.1576 1677 +1679 3 432.3348 574.5031 40.9886 0.1877 1678 +1680 3 433.4559 574.5557 41.0894 0.169 1679 +1681 3 434.5816 574.4356 41.2493 0.1536 1680 +1682 3 435.7038 574.5317 41.4481 0.1441 1681 +1683 3 436.8341 574.6026 41.5332 0.1499 1682 +1684 3 437.9575 574.7319 41.5848 0.1616 1683 +1685 3 438.9974 574.7582 41.7606 0.1771 1684 +1686 3 440.1037 574.598 41.8611 0.2367 1685 +1687 3 441.1905 574.6495 41.7578 0.1976 1686 +1688 3 442.275 574.8028 41.5299 0.1645 1687 +1689 3 443.403 574.9687 41.3081 0.1407 1688 +1690 3 444.539 575.0728 41.0906 0.1435 1689 +1691 3 445.6555 575.2798 40.8862 0.1494 1690 +1692 3 446.7343 575.6528 40.7711 0.1571 1691 +1693 3 447.8245 575.9605 40.77 0.1867 1692 +1694 3 448.8976 576.2694 40.7814 0.1669 1693 +1695 3 449.894 576.7487 40.7565 0.15 1694 +1696 3 451.0266 576.8803 40.7652 0.1373 1695 +1697 3 452.1111 577.0759 40.824 0.1373 1696 +1698 3 453.1052 577.6148 40.9368 0.1373 1697 +1699 3 454.1657 578.0277 41.0474 0.1373 1698 +1700 3 455.2319 578.0243 41.2381 0.1373 1699 +1701 3 456.313 577.8024 41.5005 0.1373 1700 +1702 3 457.362 577.9019 41.8564 0.1373 1701 +1703 3 458.4363 577.9442 42.226 0.1373 1702 +1704 3 459.57 577.903 42.4668 0.1373 1703 +1705 3 460.6842 577.752 42.5729 0.1373 1704 +1706 3 461.8145 577.6319 42.6185 0.1373 1705 +1707 3 462.9139 577.8127 42.7146 0.1372 1706 +1708 3 464.019 578.0655 42.8467 0.1372 1707 +1709 3 465.1355 578.308 42.9965 0.1371 1708 +1710 3 466.2395 578.6066 43.1514 0.1368 1709 +1711 3 467.3251 578.5014 43.3964 0.1364 1710 +1712 3 468.4348 578.2359 43.6164 0.1356 1711 +1713 3 469.461 577.7383 43.8122 0.1342 1712 +1714 3 470.4254 577.1274 43.9816 0.1313 1713 +1715 3 471.4607 576.6458 44.1263 0.1271 1714 +1716 3 472.4079 576.004 44.2299 0.1144 1715 +1717 3 473.5508 575.9651 44.3369 0.1144 1716 +1718 3 417.5097 569.7349 37.6188 0.2288 1664 +1719 3 418.315 569.0942 38.9651 0.1796 1718 +1720 3 418.9763 568.7304 39.5114 0.1499 1719 +1721 3 418.6422 567.9056 40.3007 0.1371 1720 +1722 3 418.8973 567.011 41.1533 0.1368 1721 +1723 3 419.514 566.0695 41.9331 0.1364 1722 +1724 3 419.9944 565.1017 42.6798 0.1356 1723 +1725 3 420.3182 564.0778 43.3765 0.1342 1724 +1726 3 420.7769 563.0734 43.9664 0.1313 1725 +1727 3 421.2837 562.0586 44.354 0.1271 1726 +1728 3 421.4473 560.9398 44.5782 0.1144 1727 +1729 3 422.2824 560.1768 44.8983 0.1144 1728 +1730 4 393.1253 563.8627 22.0803 0.1583 1 +1731 4 392.1186 563.3514 22.9469 0.1742 1730 +1732 4 391.1439 562.7553 23.6632 0.2158 1731 +1733 4 390.231 562.1147 24.3016 0.2341 1732 +1734 4 389.4496 561.2876 24.8045 0.2097 1733 +1735 4 388.7918 560.3529 25.1352 0.2191 1734 +1736 4 388.1192 559.4263 25.3121 0.2004 1735 +1737 4 387.204 558.741 25.4645 0.2259 1736 +1738 4 386.1824 558.2628 25.6558 0.2108 1737 +1739 4 385.2363 557.668 25.888 0.2571 1738 +1740 4 384.2456 557.096 26.0664 0.2115 1739 +1741 4 384.0214 557.4689 26.2556 0.2288 1740 +1742 4 383.0924 557.9013 27.1779 0.1925 1741 +1743 4 382.2893 558.5935 27.516 0.1713 1742 +1744 4 381.6556 559.4377 27.9271 0.1896 1743 +1745 4 380.976 560.2179 28.1943 0.1723 1744 +1746 4 380.4029 561.1606 28.5485 0.1597 1745 +1747 4 380.0036 562.2074 28.9554 0.1557 1746 +1748 4 379.5289 563.1374 29.4736 0.1694 1747 +1749 4 379.2532 564.2425 29.9233 0.2069 1748 +1750 4 379.1719 565.3385 30.3789 0.2174 1749 +1751 4 378.3666 566.0501 30.9263 0.1814 1750 +1752 4 377.4697 566.7136 31.4334 0.1533 1751 +1753 4 376.6014 567.432 31.8651 0.1435 1752 +1754 4 375.8887 568.1584 32.3596 0.1486 1753 +1755 4 375.375 569.0942 32.898 0.1587 1754 +1756 4 374.9883 570.1307 33.39 0.1748 1755 +1757 4 374.9883 571.2415 33.8453 0.217 1756 +1758 4 375.3189 572.31 34.2555 0.2362 1757 +1759 4 375.6942 573.3762 34.6144 0.2145 1758 +1760 4 375.6747 574.4859 34.8936 0.2247 1759 +1761 4 375.6221 575.6173 35.0672 0.2265 1760 +1762 4 375.6324 576.7442 35.138 0.1984 1761 +1763 4 375.7388 577.8722 35.1669 0.1857 1762 +1764 4 375.4425 578.8365 35.3587 0.1986 1763 +1765 4 375.4013 579.9302 35.6345 0.2743 1764 +1766 4 375.5878 581.0548 35.8898 0.278 1765 +1767 4 375.105 581.5204 36.374 0.2619 1766 +1768 4 375.6873 582.4745 36.6752 0.1806 1767 +1769 4 376.2124 583.4331 36.7612 0.1518 1768 +1770 4 376.5556 584.5074 36.7805 0.1407 1769 +1771 4 376.9388 585.5758 36.8214 0.1435 1770 +1772 4 377.8037 586.1158 36.9914 0.1494 1771 +1773 4 378.0291 587.1855 36.9852 0.1571 1772 +1774 4 378.2819 588.2311 36.9317 0.1867 1773 +1775 4 378.5954 589.2618 36.9863 0.167 1774 +1776 4 378.664 590.3726 37.0418 0.1501 1775 +1777 4 378.6457 591.4995 37.1703 0.1375 1776 +1778 4 378.5176 592.576 37.3915 0.1376 1777 +1779 4 378.0417 593.4855 37.5777 0.138 1778 +1780 4 377.6939 594.5162 37.7308 0.1385 1779 +1781 4 377.3644 595.5367 37.9422 0.1397 1780 +1782 4 377.1757 596.6463 38.0822 0.1417 1781 +1783 4 376.8393 597.6634 37.9764 0.1456 1782 +1784 4 376.2696 598.6323 37.8042 0.1526 1783 +1785 4 375.8303 599.5361 37.476 0.1668 1784 +1786 4 375.4333 600.4536 36.9583 0.1867 1785 +1787 4 375.1451 601.5289 36.4731 0.255 1786 +1788 4 374.8865 602.6432 36.0903 0.2295 1787 +1789 4 374.5067 603.6877 35.7294 0.2337 1788 +1790 4 374.1498 604.7584 35.4872 0.2198 1789 +1791 4 373.7562 605.8292 35.3248 0.1859 1790 +1792 4 373.5629 606.9275 35.1453 0.1621 1791 +1793 4 373.341 608.0291 34.9418 0.1571 1792 +1794 4 373.0344 609.1102 34.8572 0.1867 1793 +1795 4 372.8616 610.2382 34.823 0.1669 1794 +1796 4 372.8445 611.3811 34.811 0.15 1795 +1797 4 372.8445 612.5251 34.8267 0.1373 1796 +1798 4 372.8445 613.6691 34.8662 0.1373 1797 +1799 4 372.8445 614.8131 34.9107 0.1373 1798 +1800 4 372.7518 615.9525 34.9454 0.1373 1799 +1801 4 372.6294 617.0896 34.984 0.1373 1800 +1802 4 372.3674 617.9613 35.2223 0.1373 1801 +1803 4 371.8801 618.9223 35.2797 0.1373 1802 +1804 4 371.7039 620.0194 35.2327 0.1374 1803 +1805 4 371.7863 621.0479 35.2013 0.1374 1804 +1806 4 371.8869 622.1072 35.2344 0.1376 1805 +1807 4 371.8572 623.2409 35.2758 0.1379 1806 +1808 4 371.9007 624.3815 35.362 0.1383 1807 +1809 4 371.7005 625.4831 35.4833 0.1392 1808 +1810 4 371.3424 626.4315 35.7692 0.1409 1809 +1811 4 370.9042 627.4211 36.0903 0.1439 1810 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1832 +1834 4 366.5147 652.2413 39.3036 0.1373 1833 +1835 4 365.9953 652.9769 39.5343 0.1373 1834 +1836 4 365.413 653.9161 39.8149 0.1373 1835 +1837 4 364.9166 654.8142 40.224 0.1373 1836 +1838 4 365.1053 655.8815 40.6907 0.1373 1837 +1839 4 365.1499 656.9717 41.207 0.1373 1838 +1840 4 364.8113 658.0402 41.7309 0.1372 1839 +1841 4 364.1238 658.8628 42.2789 0.1372 1840 +1842 4 363.3824 659.7059 42.8106 0.1371 1841 +1843 4 362.8802 660.692 43.3552 0.1368 1842 +1844 4 362.6205 661.7068 43.8914 0.1364 1843 +1845 4 362.7075 662.8313 44.3271 0.1356 1844 +1846 4 362.4489 663.8163 44.7488 0.1342 1845 +1847 4 362.1149 664.7978 45.2315 0.1313 1846 +1848 4 361.8918 665.8961 45.6795 0.1271 1847 +1849 4 361.4697 666.8754 46.1409 0.1144 1848 +1850 4 360.932 667.8672 46.76 0.1144 1849 +1851 4 374.2665 582.3292 37.4346 0.1797 1767 +1852 4 373.373 582.9058 37.4069 0.1502 1851 +1853 4 372.3869 583.289 37.4007 0.1376 1852 +1854 4 371.808 584.266 37.4469 0.138 1853 +1855 4 371.2966 585.283 37.5701 0.1387 1854 +1856 4 370.8127 586.3114 37.7633 0.1398 1855 +1857 4 370.2808 587.1774 38.1111 0.142 1856 +1858 4 369.623 588.0252 38.5277 0.146 1857 +1859 4 369.0224 588.9953 38.8786 0.1532 1858 +1860 4 368.4698 589.9974 39.1574 0.1677 1859 +1861 4 367.8246 590.9218 39.4262 0.1889 1860 +1862 4 367.4539 591.9639 39.5867 0.2559 1861 +1863 4 367.4665 593.0839 39.7911 0.2461 1862 +1864 4 367.4951 594.205 39.968 0.1923 1863 +1865 4 367.0638 595.2324 40.1391 0.15 1864 +1866 4 366.7138 596.2688 40.423 0.1373 1865 +1867 4 366.493 597.3728 40.7198 0.1373 1866 +1868 4 366.2402 598.4619 40.9735 0.1373 1867 +1869 4 366.0811 599.4629 41.3258 0.1373 1868 +1870 4 365.9736 600.5886 41.641 0.1373 1869 +1871 4 365.3112 601.4683 41.9093 0.1373 1870 +1872 4 364.8902 602.4453 42.2358 0.1373 1871 +1873 4 364.5596 603.5321 42.497 0.1373 1872 +1874 4 364.0986 604.5228 42.8168 0.1373 1873 +1875 4 364.0208 605.6359 43.0833 0.1373 1874 +1876 4 363.7337 606.7158 43.2426 0.1373 1875 +1877 4 363.7691 607.7557 43.468 0.1373 1876 +1878 4 363.673 608.6492 43.4669 0.1373 1877 +1879 4 363.8298 609.6582 43.601 0.1373 1878 +1880 4 363.5804 610.3423 44.1 0.1373 1879 +1881 4 363.3527 611.2998 44.7924 0.1373 1880 +1882 4 363.0209 612.2539 45.6173 0.1373 1881 +1883 4 363.1628 613.3499 46.4206 0.1373 1882 +1884 4 363.0507 614.4367 47.194 0.1373 1883 +1885 4 362.7624 615.5292 47.7988 0.1373 1884 +1886 4 362.7613 616.6732 48.2143 0.1373 1885 +1887 4 362.6297 617.6548 48.5705 0.1373 1886 +1888 4 362.1229 618.3183 48.9272 0.1373 1887 +1889 4 361.6024 619.2735 49.191 0.1373 1888 +1890 4 361.0967 620.2665 49.4735 0.1373 1889 +1891 4 361.0155 621.3956 49.6502 0.1373 1890 +1892 4 360.5568 622.4378 49.8229 0.1373 1891 +1893 4 360.082 623.4731 49.982 0.1373 1892 +1894 4 359.4711 624.4364 50.1169 0.1373 1893 +1895 4 358.7195 625.2955 50.2256 0.1374 1894 +1896 4 357.961 626.1524 50.3098 0.1374 1895 +1897 4 357.3307 627.1053 50.3944 0.1375 1896 +1898 4 356.7209 628.0732 50.4753 0.1376 1897 +1899 4 356.1112 629.041 50.5621 0.138 1898 +1900 4 355.5026 630.0088 50.666 0.1385 1899 +1901 4 354.8928 630.9766 50.7928 0.1397 1900 +1902 4 354.3014 631.9559 50.9454 0.1417 1901 +1903 4 353.9364 633.0347 51.1274 0.1453 1902 +1904 4 353.6184 634.1043 51.3985 0.1528 1903 +1905 4 353.5898 635.1568 51.7888 0.1632 1904 +1906 4 353.1505 636.1784 52.1606 0.1988 1905 +1907 4 353.1803 637.0753 52.5932 0.1868 1906 +1908 4 353.8724 637.9047 52.7954 0.1995 1907 +1909 4 354.187 638.8954 52.8209 0.1671 1908 +1910 4 354.3128 639.8781 52.9469 0.1503 1909 +1911 4 354.0977 640.815 53.2655 0.138 1910 +1912 4 354.0074 641.8892 53.6189 0.1385 1911 +1913 4 353.8426 642.8502 53.7807 0.1396 1912 +1914 4 353.6779 643.7162 54.1422 0.1414 1913 +1915 4 353.3187 644.1715 54.7478 0.1449 1914 +1916 4 353.5269 645.24 55.1508 0.1514 1915 +1917 4 353.9216 646.1564 55.5391 0.1645 1916 +1918 4 353.7431 647.2752 55.7981 0.1822 1917 +1919 4 353.5475 648.3906 55.9922 0.2471 1918 +1920 4 353.3438 649.2532 55.8816 0.2119 1919 +1921 4 353.0132 650.2096 55.7208 0.2161 1920 +1922 4 353.2672 651.2666 55.1911 0.1144 1921 +1923 4 383.4013 556.3981 26.2028 0.1914 1740 +1924 4 382.549 555.6362 26.3007 0.1851 1923 +1925 4 381.6361 554.9464 26.3656 0.2521 1924 +1926 4 380.9451 554.0346 26.4089 0.2234 1925 +1927 4 380.2404 553.1332 26.4526 0.2258 1926 +1928 4 379.6078 552.1802 26.5114 0.1897 1927 +1929 4 378.9843 551.249 26.5202 0.205 1928 +1930 4 378.4455 550.2983 26.6169 0.1772 1929 +1931 4 377.8941 549.4335 26.9217 0.1689 1930 +1932 4 377.3587 548.5228 27.352 0.1733 1931 +1933 4 376.9102 547.4818 27.7455 0.1993 1932 +1934 4 376.2181 546.578 28.028 0.2755 1933 +1935 4 375.3018 545.9431 28.1551 0.2803 1934 +1936 4 374.4392 545.2235 28.2934 0.2661 1935 +1937 4 373.8432 544.2614 28.4206 0.2373 1936 +1938 4 373.3272 543.241 28.5163 0.1955 1937 +1939 4 372.7827 542.2354 28.609 0.1765 1938 +1940 4 372.1306 541.295 28.7081 0.1999 1939 +1941 4 371.5094 540.3352 28.8123 0.1886 1940 +1942 4 370.8711 539.3891 28.9279 0.2031 1941 +1943 4 370.1572 538.546 29.1309 0.1735 1942 +1944 4 369.3839 537.7086 29.3163 0.1628 1943 +1945 4 368.6963 536.7957 29.4546 0.1583 1944 +1946 4 368.0248 535.869 29.5484 0.1891 1945 +1947 4 367.4322 534.8898 29.603 0.1713 1946 +1948 4 367.0192 533.8247 29.6204 0.1581 1947 +1949 4 366.5776 532.7722 29.5932 0.1523 1948 +1950 4 366.247 531.69 29.5162 0.1653 1949 +1951 4 366.0708 530.5849 29.549 0.1876 1950 +1952 4 365.9702 529.4478 29.6024 0.2382 1951 +1953 4 365.5812 528.3736 29.659 0.2879 1952 +1954 4 364.9383 527.4286 29.72 0.2507 1953 +1955 4 364.1524 526.5981 29.7881 0.2382 1954 +1956 4 363.3516 525.7813 29.8603 0.2826 1955 +1957 4 362.5748 524.9416 29.9312 0.3805 1956 +1958 4 361.8998 524.0195 30.0292 0.3716 1957 +1959 4 361.3484 523.0379 30.1977 0.3651 1958 +1960 4 360.7398 522.085 30.403 0.4192 1959 +1961 4 360.2227 521.0645 30.5732 0.4261 1960 +1962 4 359.8475 519.9846 30.704 0.4345 1961 +1963 4 359.4036 518.9344 30.7824 0.3582 1962 +1964 4 359.0421 517.8579 30.8039 0.3195 1963 +1965 4 358.5124 516.8729 30.9224 0.3204 1964 +1966 4 357.7414 516.0367 31.0811 0.3125 1965 +1967 4 357.0424 515.1695 31.3216 0.2299 1966 +1968 4 356.5093 514.1605 31.5605 0.178 1967 +1969 4 356.1386 513.0794 31.7657 0.163 1968 +1970 4 356.1203 511.9423 31.9141 0.1977 1969 +1971 4 356.2851 510.9642 31.8646 0.1876 1970 +1972 4 355.8366 510.7605 32.0118 0.1862 1971 +1973 4 355.1159 510.2904 32.4204 0.2147 1972 +1974 4 354.4867 509.4221 32.9154 0.2315 1973 +1975 4 353.8609 508.4748 33.329 0.2082 1974 +1976 4 353.3919 507.4372 33.6818 0.2007 1975 +1977 4 353.0212 506.379 34.0301 0.2417 1976 +1978 4 352.5728 505.3323 34.2426 0.2801 1977 +1979 4 352.1747 504.2615 34.3403 0.3062 1978 +1980 4 351.7056 503.2227 34.4361 0.3432 1979 +1981 4 351.2263 502.184 34.5184 0.3533 1980 +1982 4 350.7115 501.1635 34.5811 0.3132 1981 +1983 4 350.1498 500.1808 34.6962 0.2954 1982 +1984 4 349.7048 499.1432 34.8636 0.2168 1983 +1985 4 349.3284 498.0644 35.0179 0.1771 1984 +1986 4 348.8319 497.0348 35.1327 0.1614 1985 +1987 4 348.2851 496.0292 35.2153 0.1948 1986 +1988 4 347.6216 495.098 35.268 0.1818 1987 +1989 4 346.9443 494.176 35.2929 0.1786 1988 +1990 4 346.2236 493.2882 35.3035 0.185 1989 +1991 4 345.5784 492.3433 35.3133 0.2518 1990 +1992 4 345.218 491.2599 35.3265 0.2229 1991 +1993 4 345.3484 490.1274 35.3447 0.2241 1992 +1994 4 345.4228 488.9856 35.3704 0.1897 1993 +1995 4 345.1585 487.8737 35.4077 0.19 1994 +1996 4 344.6231 486.8624 35.4584 0.2216 1995 +1997 4 344.1358 485.8271 35.5256 0.2447 1996 +1998 4 343.6324 484.7997 35.6166 0.2306 1997 +1999 4 343.1577 483.7736 35.7764 0.2525 1998 +2000 4 342.6291 482.7794 35.9948 0.2883 1999 +2001 4 342.2676 481.6949 36.1967 0.2638 2000 +2002 4 341.9599 480.5944 36.3653 0.2016 2001 +2003 4 341.7631 479.4676 36.5047 0.1676 2002 +2004 4 341.4074 478.3808 36.6229 0.1673 2003 +2005 4 340.8399 477.3901 36.7424 0.206 2004 +2006 4 340.2908 476.4142 36.9298 0.2008 2005 +2007 4 339.8893 475.348 37.1185 0.2229 2006 +2008 4 339.434 474.3104 37.2249 0.2231 2007 +2009 4 338.926 473.2888 37.3545 0.1925 2008 +2010 4 338.4295 472.2649 37.5141 0.1714 2009 +2011 4 338.0142 471.201 37.6684 0.1897 2010 +2012 4 337.448 470.2126 37.84 0.1724 2011 +2013 4 336.8268 469.2608 37.9714 0.1599 2012 +2014 4 336.1438 468.3753 38.199 0.1562 2013 +2015 4 335.4597 467.4807 38.4667 0.1702 2014 +2016 4 334.9003 466.4843 38.7005 0.2084 2015 +2017 4 334.4507 465.433 38.922 0.2201 2016 +2018 4 333.9382 464.4228 39.1768 0.1865 2017 +2019 4 333.365 463.4538 39.4772 0.1631 2018 +2020 4 332.8388 462.4414 39.7592 0.1591 2019 +2021 4 332.3869 461.3912 40.0162 0.1905 2020 +2022 4 331.887 460.3673 40.2856 0.1739 2021 +2023 4 331.4546 459.3343 40.6104 0.1634 2022 +2024 4 331.1171 458.2601 40.9609 0.1594 2023 +2025 4 330.7842 457.1893 41.3356 0.191 2024 +2026 4 330.362 456.1368 41.6956 0.1751 2025 +2027 4 329.9067 455.0878 41.9986 0.1649 2026 +2028 4 329.3382 454.0994 42.2579 0.166 2027 +2029 4 328.6769 453.1864 42.5267 0.1851 2028 +2030 4 327.9596 452.3136 42.7966 0.252 2029 +2031 4 327.3087 451.3744 43.027 0.2235 2030 +2032 4 326.7447 450.3791 43.2244 0.2254 2031 +2033 4 326.1567 449.3998 43.4053 0.1918 2032 +2034 4 325.468 448.4869 43.5809 0.1939 2033 +2035 4 324.7599 447.6083 43.7917 0.2294 2034 +2036 4 323.9831 446.8556 44.1182 0.2567 2035 +2037 4 323.2006 446.0822 44.4954 0.2645 2036 +2038 4 322.5748 445.1338 44.7972 0.2584 2037 +2039 4 322.1195 444.1065 44.9854 0.2323 2038 +2040 4 321.7878 443.0174 45.1688 0.2558 2039 +2041 4 321.3599 441.9821 45.3984 0.2944 2040 +2042 4 320.7822 441.0097 45.6476 0.275 2041 +2043 4 320.1999 440.0499 45.9376 0.2225 2042 +2044 4 319.5924 439.0912 46.2272 0.2066 2043 +2045 4 318.9255 438.1726 46.499 0.2385 2044 +2046 4 318.342 437.2093 46.7524 0.3429 2045 +2047 4 317.786 436.2175 46.9042 0.4315 2046 +2048 4 317.1488 435.292 46.935 0.4186 2047 +2049 4 316.3869 434.4432 46.9297 0.4543 2048 +2050 4 315.5976 433.6149 46.9428 0.4601 2049 +2051 4 314.8723 432.7455 47.0126 0.4197 2050 +2052 4 314.274 431.7936 47.1657 0.3653 2051 +2053 4 313.7145 430.8018 47.3508 0.2791 2052 +2054 4 313.0773 429.8569 47.5264 0.2762 2053 +2055 4 312.3131 429.0057 47.6806 0.3114 2054 +2056 4 311.5123 428.1889 47.8173 0.2926 2055 +2057 4 310.7207 427.3641 47.9469 0.3235 2056 +2058 4 310.008 426.4786 48.0959 0.405 2057 +2059 4 309.4852 425.4799 48.3112 0.4384 2058 +2060 4 309.0962 424.4286 48.5951 0.3845 2059 +2061 4 308.7336 423.3612 48.9096 0.2751 2060 +2062 4 308.3332 422.3168 49.2447 0.2328 2061 +2063 4 307.7909 421.3146 49.5261 0.291 2062 +2064 4 307.1571 420.3628 49.7367 0.3065 2063 +2065 4 306.5142 419.4156 49.8876 0.3272 2064 +2066 4 305.853 418.4832 49.9971 0.2892 2065 +2067 4 305.1483 417.5829 50.0906 0.2466 2066 +2068 4 304.4207 416.7112 50.2088 0.2617 2067 +2069 4 303.597 415.9321 50.3826 0.2903 2068 +2070 4 302.8031 415.1221 50.545 0.3407 2069 +2071 4 302.1521 414.2161 50.68 0.3331 2070 +2072 4 301.7437 413.1968 50.9169 0.351 2071 +2073 4 301.3399 412.2015 51.2347 0.3435 2072 +2074 4 300.7096 411.2566 51.5175 0.2977 2073 +2075 4 300.1627 410.2613 51.7059 0.2542 2074 +2076 4 299.7234 409.2122 51.8182 0.2 2075 +2077 4 299.2544 408.1723 51.9201 0.2005 2076 +2078 4 298.7648 407.1427 51.9996 0.169 2077 +2079 4 298.2374 406.128 52.0484 0.1536 2078 +2080 4 297.7088 405.1133 52.0946 0.1441 2079 +2081 4 297.194 404.0917 52.1455 0.1497 2080 +2082 4 296.7467 403.0392 52.197 0.1614 2081 +2083 4 296.3006 401.9867 52.2502 0.1769 2082 +2084 4 295.756 400.9834 52.327 0.2362 2083 +2085 4 295.1783 399.9996 52.4322 0.1968 2084 +2086 4 294.7138 398.9551 52.542 0.163 2085 +2087 4 294.2643 397.9107 52.6579 0.1379 2086 +2088 4 293.6362 396.968 52.8209 0.1383 2087 +2089 4 292.9738 396.0448 52.9976 0.1391 2088 +2090 4 292.2874 395.1296 53.1387 0.1407 2089 +2091 4 291.601 394.2144 53.2543 0.1436 2090 +2092 4 290.9146 393.2992 53.3551 0.1495 2091 +2093 4 290.2271 392.384 53.447 0.1574 2092 +2094 4 289.5453 391.4665 53.5399 0.1874 2093 +2095 4 288.9023 390.5307 53.6757 0.1683 2094 +2096 4 288.3463 389.5595 53.8969 0.1525 2095 +2097 4 287.8396 388.5436 54.1346 0.1419 2096 +2098 4 287.3842 387.4945 54.3402 0.1456 2097 +2099 4 286.9198 386.4524 54.539 0.1534 2098 +2100 4 286.421 385.433 54.7509 0.1645 2099 +2101 4 285.8044 384.4778 54.9206 0.201 2100 +2102 4 285.0756 383.6084 55.0304 0.1908 2101 +2103 4 284.3847 382.7058 55.1877 0.2071 2102 +2104 4 283.7131 381.7997 55.4058 0.181 2103 +2105 4 283.0393 380.8822 55.6413 0.1766 2104 +2106 4 282.377 379.9579 55.8928 0.1843 2105 +2107 4 281.7535 379.0129 56.1674 0.2357 2106 +2108 4 281.2055 378.0176 56.4281 0.2652 2107 +2109 4 280.7811 376.956 56.6272 0.2962 2108 +2110 4 280.3612 375.8967 56.7966 0.2392 2109 +2111 4 279.946 374.8465 56.9898 0.2315 2110 +2112 4 279.454 373.8272 57.1791 0.2002 2111 +2113 4 278.8694 372.8536 57.3054 0.2254 2112 +2114 4 278.4061 371.8332 57.3286 0.2099 2113 +2115 4 277.944 370.8116 57.3555 0.2551 2114 +2116 4 277.2701 369.925 57.4818 0.2082 2115 +2117 4 276.5609 369.0452 57.6388 0.1846 2116 +2118 4 275.9911 368.0534 57.7898 0.1758 2117 +2119 4 275.4397 367.0524 57.944 0.2187 2118 +2120 4 274.8597 366.0789 58.1207 0.2396 2119 +2121 4 274.091 365.2632 58.3537 0.2204 2120 +2122 4 273.2627 364.5013 58.6104 0.2364 2121 +2123 4 272.5008 363.649 58.8426 0.2455 2122 +2124 4 271.8167 362.7361 59.0579 0.247 2123 +2125 4 271.1944 361.7786 59.2693 0.21 2124 +2126 4 270.5388 360.8702 59.5238 0.2195 2125 +2127 4 269.9199 359.9516 59.8167 0.2021 2126 +2128 4 269.4498 358.914 60.06 0.2255 2127 +2129 4 268.9452 357.9004 60.251 0.2279 2128 +2130 4 268.2829 356.968 60.4243 0.2016 2129 +2131 4 267.5953 356.1398 60.6628 0.188 2130 +2132 4 266.8735 355.3767 60.979 0.2207 2131 +2133 4 266.2122 354.4581 61.2002 0.2283 2132 +2134 4 265.6219 353.4868 61.3575 0.2717 2133 +2135 4 264.9882 352.5362 61.5037 0.3248 2134 +2136 4 264.3269 351.6084 61.6613 0.3273 2135 +2137 4 263.6828 350.6829 61.8646 0.3398 2136 +2138 4 262.9884 349.7906 62.0942 0.3254 2137 +2139 4 262.3055 348.8777 62.3179 0.2511 2138 +2140 4 261.6683 347.9304 62.5408 0.2299 2139 +2141 4 261.0185 347.0061 62.7956 0.1973 2140 +2142 4 260.3778 346.0749 63.0652 0.2194 2141 +2143 4 259.7589 345.1151 63.3097 0.202 2142 +2144 4 259.1835 344.1278 63.5281 0.2251 2143 +2145 4 258.6561 343.1119 63.7297 0.2273 2144 +2146 4 258.0841 342.1326 63.9545 0.2007 2145 +2147 4 257.1884 341.6533 64.246 0.186 2146 +2148 4 256.081 341.452 64.568 0.2178 2147 +2149 4 255.064 341.0058 64.918 0.2199 2148 +2150 4 254.206 340.2839 65.2641 0.271 2149 +2151 4 253.5001 339.3905 65.5477 0.2504 2150 +2152 4 252.9762 338.3849 65.7838 0.2005 2151 +2153 4 252.6249 337.313 66.0058 0.1651 2152 +2154 4 252.2692 336.2365 66.1973 0.1657 2153 +2155 4 251.8527 335.1726 66.3309 0.1884 2154 +2156 4 251.3963 334.1258 66.3894 0.2394 2155 +2157 4 250.9147 333.1282 66.3228 0.2907 2156 +2158 4 250.7499 332.0368 66.2152 0.2539 2157 +2159 4 250.7442 330.894 66.145 0.2527 2158 +2160 4 250.3896 329.8152 66.1343 0.2678 2159 +2161 4 249.9903 328.7433 66.1884 0.2129 2160 +2162 4 249.5911 327.6725 66.306 0.1694 2161 +2163 4 249.1918 326.6017 66.4779 0.1497 2162 +2164 4 248.7937 325.5355 66.7058 0.1608 2163 +2165 4 248.399 324.4819 66.9992 0.1782 2164 +2166 4 248.0032 323.4294 67.3406 0.2264 2165 +2167 4 247.6085 322.3781 67.7099 0.2389 2166 +2168 4 247.1932 321.3187 68.0576 0.2917 2167 +2169 4 246.7597 320.2605 68.3539 0.3591 2168 +2170 4 246.3261 319.2023 68.6059 0.4041 2169 +2171 4 245.8925 318.1441 68.8274 0.4185 2170 +2172 4 245.404 317.1145 69.0603 0.4366 2171 +2173 4 244.816 316.1673 69.3602 0.3978 2172 +2174 4 244.2177 315.2281 69.7172 0.3328 2173 +2175 4 243.6194 314.2877 70.11 0.2937 2174 +2176 4 243.0176 313.3199 70.4511 0.2833 2175 +2177 4 242.4159 312.3475 70.7154 0.3061 2176 +2178 4 241.8164 311.3728 70.9125 0.2582 2177 +2179 4 241.2158 310.3992 71.0598 0.2645 2178 +2180 4 240.6232 309.4223 71.2037 0.2722 2179 +2181 4 240.0364 308.4453 71.3714 0.3059 2180 +2182 4 239.4495 307.4683 71.5716 0.2734 2181 +2183 4 238.9576 306.4456 71.8105 0.2169 2182 +2184 4 238.5492 305.3919 72.0835 0.2083 2183 +2185 4 238.1488 304.3337 72.371 0.1835 2184 +2186 4 237.7495 303.2767 72.655 0.181 2185 +2187 4 237.3686 302.199 72.868 0.193 2186 +2188 4 236.9968 301.1168 73.0156 0.2486 2187 +2189 4 236.6764 300.0186 73.1114 0.3051 2188 +2190 4 236.2829 298.9455 73.171 0.2922 2189 +2191 4 235.5302 298.0909 73.2116 0.2674 2190 +2192 4 234.6516 297.3599 73.2497 0.2256 2191 +2193 4 233.6712 296.7719 73.3006 0.2426 2192 +2194 4 232.7457 296.1004 73.3687 0.2727 2193 +2195 4 231.9243 295.3064 73.4734 0.2219 2194 +2196 4 231.1681 294.4519 73.6254 0.1872 2195 +2197 4 230.4165 293.5939 73.8189 0.1774 2196 +2198 4 229.6649 292.7347 74.0488 0.2372 2197 +2199 4 229.0906 291.7566 74.3302 0.1985 2198 +2200 4 228.5792 290.7465 74.6578 0.1662 2199 +2201 4 228.0724 289.734 75.0165 0.1438 2200 +2202 4 227.5656 288.7216 75.3939 0.1499 2201 +2203 4 227.044 287.7274 75.7985 0.158 2202 +2204 4 226.52 286.7356 76.2143 0.1884 2203 +2205 4 225.9961 285.7437 76.6242 0.17 2204 +2206 4 225.519 284.7199 77.0017 0.1557 2205 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2271 +2273 4 204.9362 217.1095 89.9136 0.1373 2272 +2274 4 205.0746 215.9975 89.7131 0.1373 2273 +2275 4 205.1558 215.0251 89.3334 0.1373 2274 +2276 4 205.3045 213.9772 89.1321 0.1373 2275 +2277 4 205.3102 213.3194 89.2206 0.1373 2276 +2278 4 205.8822 212.7028 89.1276 0.1373 2277 +2279 4 206.3993 211.8734 88.851 0.1373 2278 +2280 4 206.4485 210.7545 88.6217 0.1373 2279 +2281 4 206.4485 209.6105 88.4514 0.1373 2280 +2282 4 206.7757 208.5318 88.3476 0.1373 2281 +2283 4 207.0846 207.445 88.2022 0.1373 2282 +2284 4 207.9003 206.6544 88.116 0.1373 2283 +2285 4 208.8097 205.9612 88.0642 0.1373 2284 +2286 4 209.5808 205.1169 88.0057 0.1373 2285 +2287 4 210.2764 204.2086 87.9337 0.1373 2286 +2288 4 211.0062 203.3288 87.8489 0.1373 2287 +2289 4 211.7784 202.4857 87.7405 0.1373 2288 +2290 4 212.4385 201.5648 87.5792 0.1373 2289 +2291 4 213.0917 200.6999 87.3396 0.1373 2290 +2292 4 213.2096 199.6028 87.0873 0.1374 2291 +2293 4 213.8365 198.6545 86.917 0.1374 2292 +2294 4 214.1408 197.5596 86.8546 0.1376 2293 +2295 4 214.246 196.4237 86.8697 0.1379 2294 +2296 4 214.3376 195.2842 86.9263 0.1383 2295 +2297 4 214.3902 194.1494 87.0075 0.1391 2296 +2298 4 214.2312 193.0351 87.1203 0.1407 2297 +2299 4 214.206 191.9071 87.2138 0.1435 2298 +2300 4 214.0596 190.7803 87.1856 0.1494 2299 +2301 4 214.0115 189.6821 86.9856 0.1571 2300 +2302 4 214.0527 188.6078 86.641 0.1867 2301 +2303 4 214.2838 187.4924 86.312 0.1669 2302 +2304 4 214.4851 186.3816 86.0418 0.15 2303 +2305 4 214.6647 185.2742 85.736 0.1373 2304 +2306 4 215.0171 184.1909 85.4515 0.1373 2305 +2307 4 215.2413 183.0755 85.1578 0.1373 2306 +2308 4 215.509 181.9715 84.8686 0.1373 2307 +2309 4 215.9849 180.9819 84.5244 0.1373 2308 +2310 4 216.1931 180.2372 83.9339 0.1373 2309 +2311 4 216.0696 179.4147 83.356 0.1373 2310 +2312 4 216.3224 178.3885 83.0147 0.1373 2311 +2313 4 216.621 177.3143 82.7193 0.1373 2312 +2314 4 216.7651 176.2321 82.3432 0.1373 2313 +2315 4 217.3451 175.5376 81.8278 0.1373 2314 +2316 4 218.0556 174.7838 81.5091 0.1373 2315 +2317 4 218.3015 173.7404 81.1798 0.1373 2316 +2318 4 218.6996 173.0883 80.666 0.1373 2317 +2319 4 218.9616 172.3024 80.1576 0.1373 2318 +2320 4 218.9113 171.1664 79.8095 0.1373 2319 +2321 4 219.1126 170.122 79.735 0.1373 2320 +2322 4 219.5004 169.0752 79.8277 0.1373 2321 +2323 4 220.0244 168.0902 80.0733 0.1373 2322 +2324 4 220.0392 167.0263 80.4572 0.1373 2323 +2325 4 220.2898 165.9235 80.7514 0.1373 2324 +2326 4 220.3344 164.8001 80.9262 0.1373 2325 +2327 4 220.3367 163.6881 81.1143 0.1373 2326 +2328 4 220.7119 162.8713 81.317 0.1373 2327 +2329 4 221.5745 162.2501 81.3753 0.1373 2328 +2330 4 221.8845 161.1999 81.3112 0.1373 2329 +2331 4 221.6958 160.1154 81.2694 0.1373 2330 +2332 4 221.7507 159.0171 81.2235 0.1373 2331 +2333 4 221.4921 158.0287 80.9827 0.1373 2332 +2334 4 221.6592 156.9202 80.6946 0.1373 2333 +2335 4 221.7083 155.8059 80.3947 0.1373 2334 +2336 4 221.7095 154.758 80.3163 0.1373 2335 +2337 4 221.944 153.7433 80.2939 0.1373 2336 +2338 4 222.2094 152.6874 80.1965 0.1373 2337 +2339 4 222.1534 151.5663 80.0117 0.1373 2338 +2340 4 222.0229 150.5149 79.641 0.1373 2339 +2341 4 222.1316 149.5071 79.0916 0.1373 2340 +2342 4 222.317 148.3985 78.5294 0.1373 2341 +2343 4 222.8569 147.4044 78.0363 0.1372 2342 +2344 4 223.223 146.3393 77.5648 0.1372 2343 +2345 4 223.557 145.2491 77.1492 0.1371 2344 +2346 4 223.7664 144.1863 76.6878 0.1368 2345 +2347 4 224.0432 143.2196 76.1183 0.1356 2346 +2348 4 224.089 142.1431 75.6056 0.1342 2347 +2349 4 224.0124 141.0014 75.2268 0.1313 2348 +2350 4 223.9369 139.8609 74.9708 0.1271 2349 +2351 4 223.8602 138.7192 74.8171 0.1144 2350 +2352 4 223.7847 137.5774 74.7376 0.1144 2351 +2353 4 201.1026 230.3924 92.12 0.2039 2258 +2354 4 200.589 229.4212 92.4202 0.1896 2353 +2355 4 200.0524 228.474 92.5288 0.2361 2354 +2356 4 199.7721 227.3906 92.5652 0.1967 2355 +2357 4 199.5616 226.2672 92.6184 0.1627 2356 +2358 4 199.0503 225.2605 92.7475 0.1373 2357 +2359 4 198.5012 224.5866 93.142 0.1373 2358 +2360 4 198.0539 223.5616 93.5656 0.1373 2359 +2361 4 197.4922 222.5972 94.0159 0.1373 2360 +2362 4 196.9488 221.5939 94.3365 0.1373 2361 +2363 4 196.4351 220.5975 94.4684 0.1373 2362 +2364 4 195.9363 219.5805 94.5801 0.1373 2363 +2365 4 195.4455 218.5715 94.6907 0.1373 2364 +2366 4 195.306 217.9206 94.726 0.1373 2365 +2367 4 195.0497 216.8074 94.7173 0.1373 2366 +2368 4 194.3027 216.2755 94.6719 0.1373 2367 +2369 4 193.2124 216.041 94.5748 0.1373 2368 +2370 4 192.2481 215.4724 94.4969 0.1373 2369 +2371 4 191.294 214.8478 94.5137 0.1373 2370 +2372 4 190.3696 214.2071 94.6702 0.1373 2371 +2373 4 189.5391 213.467 94.9595 0.1373 2372 +2374 4 188.8733 212.5609 95.303 0.1373 2373 +2375 4 188.1171 211.8082 95.7438 0.1373 2374 +2376 4 187.1252 211.3643 96.2242 0.1373 2375 +2377 4 186.4148 210.5749 96.4914 0.1373 2376 +2378 4 185.4172 210.0659 96.7403 0.1373 2377 +2379 4 184.8281 209.781 96.9604 0.1373 2378 +2380 4 183.89 209.1335 97.1936 0.1373 2379 +2381 4 183.6944 208.1039 97.5545 0.1373 2380 +2382 4 183.1555 207.104 97.902 0.1373 2381 +2383 4 182.4794 206.238 98.3447 0.1373 2382 +2384 4 182.2895 205.1741 98.8627 0.1373 2383 +2385 4 182.0138 204.1159 99.321 0.1373 2384 +2386 4 181.9132 203.1721 99.8208 0.1373 2385 +2387 4 181.1227 202.353 100.2607 0.1373 2386 +2388 4 180.7097 201.3669 100.7356 0.1373 2387 +2389 4 180.2647 200.3682 101.2407 0.1373 2388 +2390 4 179.5336 199.5479 101.6526 0.1373 2389 +2391 4 178.5143 199.151 101.9953 0.1373 2390 +2392 4 177.4607 199.0835 102.4974 0.1373 2391 +2393 4 176.6187 199.0823 103.29 0.1373 2392 +2394 4 175.5754 199.0823 104.1782 0.1373 2393 +2395 4 174.7986 198.8032 105.2234 0.1373 2394 +2396 4 174.055 198.055 106.2538 0.1373 2395 +2397 4 173.4762 197.0826 107.1246 0.1373 2396 +2398 4 172.5461 196.5346 107.9056 0.1373 2397 +2399 4 171.489 196.1102 108.5059 0.1373 2398 +2400 4 170.3576 195.9821 108.983 0.1373 2399 +2401 4 169.2331 195.9821 109.4089 0.1373 2400 +2402 4 168.1108 195.9718 109.8098 0.1373 2401 +2403 4 167.0183 195.7739 110.2086 0.1373 2402 +2404 4 165.9258 195.5748 110.5871 0.1373 2403 +2405 4 164.9168 195.0703 110.938 0.1373 2404 +2406 4 163.87 194.7145 111.1018 0.1373 2405 +2407 4 162.8598 194.5715 111.0029 0.1373 2406 +2408 4 161.7845 194.3553 110.8579 0.1373 2407 +2409 4 160.7068 194.035 110.812 0.1373 2408 +2410 4 159.6612 193.5957 110.8285 0.1373 2409 +2411 4 158.6293 193.1209 110.8495 0.1373 2410 +2412 4 157.5997 192.6302 110.9206 0.1373 2411 +2413 4 156.577 192.1234 111.0738 0.1373 2412 +2414 4 155.6343 191.6006 111.3661 0.1373 2413 +2415 4 154.7512 191.0812 111.7973 0.1373 2414 +2416 4 153.741 190.6167 112.1996 0.1373 2415 +2417 4 152.692 190.166 112.5233 0.1373 2416 +2418 4 151.6635 189.6672 112.7322 0.1373 2417 +2419 4 150.6408 189.1558 112.8103 0.1373 2418 +2420 4 149.7633 188.4351 112.7599 0.1373 2419 +2421 4 148.9374 187.6423 112.6048 0.1373 2420 +2422 4 147.9489 187.1573 112.4511 0.1373 2421 +2423 4 147.0806 186.726 112.1123 0.1373 2422 +2424 4 147.1539 186.4102 111.3776 0.1373 2423 +2425 4 147.012 185.3555 110.6353 0.1373 2424 +2426 4 146.3416 184.4906 109.9476 0.1373 2425 +2427 4 145.59 183.9701 109.2297 0.1373 2426 +2428 4 144.7663 183.6646 108.5826 0.1373 2427 +2429 4 144.2241 183.2608 108.4264 0.1373 2428 +2430 4 143.6258 182.9233 108.6876 0.1373 2429 +2431 4 142.746 183.0022 108.8102 0.1373 2430 +2432 4 141.9361 182.4703 108.7318 0.1373 2431 +2433 4 141.403 181.5814 108.7593 0.1373 2432 +2434 4 140.4226 181.2702 108.5885 0.1373 2433 +2435 4 139.8963 180.3779 108.2491 0.1373 2434 +2436 4 139.0921 179.8528 107.7437 0.1373 2435 +2437 4 138.3393 179.1287 107.2627 0.1373 2436 +2438 4 137.7204 178.2032 106.8872 0.1373 2437 +2439 4 136.827 177.8851 106.7746 0.1373 2438 +2440 4 135.7379 177.9881 106.7951 0.1373 2439 +2441 4 134.6133 177.8828 106.8561 0.1373 2440 +2442 4 133.5162 177.5717 106.9457 0.1373 2441 +2443 4 132.9877 176.7755 107.2095 0.1373 2442 +2444 4 132.1583 176.0753 107.5119 0.1373 2443 +2445 4 131.0658 175.7562 107.7174 0.1373 2444 +2446 4 129.9996 175.4759 107.7558 0.1373 2445 +2447 4 129.272 174.6064 107.7832 0.1373 2446 +2448 4 128.2985 174.0504 107.8246 0.1373 2447 +2449 4 127.4427 173.3 107.7541 0.1373 2448 +2450 4 126.5859 172.5507 107.6172 0.1373 2449 +2451 4 125.729 171.8013 107.4461 0.1373 2450 +2452 4 124.8733 171.0509 107.252 0.1373 2451 +2453 4 124.0165 170.3016 107.0278 0.1373 2452 +2454 4 123.0647 169.5351 106.8228 0.1373 2453 +2455 4 122.1746 168.8166 106.6394 0.1373 2454 +2456 4 121.224 168.1852 106.4636 0.1373 2455 +2457 4 120.3362 167.4724 106.2827 0.1373 2456 +2458 4 119.5446 166.7437 106.0122 0.1372 2457 +2459 4 118.8101 166.1786 105.5317 0.1372 2458 +2460 4 117.6741 166.3159 105.1078 0.1371 2459 +2461 4 116.5404 166.4692 104.7253 0.1368 2460 +2462 4 115.4147 166.4371 104.3731 0.1364 2461 +2463 4 114.2941 166.2083 104.0351 0.1356 2462 +2464 4 113.3283 166.3284 103.6266 0.1342 2463 +2465 4 112.4692 166.6957 103.0299 0.1313 2464 +2466 4 111.6101 167.0629 102.2899 0.1271 2465 +2467 4 110.521 166.8741 101.6935 0.1144 2466 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190.071 124.2637 0.1342 2488 +2490 4 186.0808 189.2176 124.3452 0.1313 2489 +2491 4 186.8472 188.4122 124.2718 0.1271 2490 +2492 4 187.9306 188.1571 124.2522 0.1144 2491 +2493 4 188.1388 187.0852 124.5524 0.1144 2492 +2494 4 184.1851 210.0659 95.359 0.1373 2378 +2495 4 183.1201 210.0052 94.3656 0.1373 2494 +2496 4 182.3067 209.5682 93.847 0.1373 2495 +2497 4 181.3846 208.891 93.3943 0.1373 2496 +2498 4 180.4786 208.1931 92.9989 0.1373 2497 +2499 4 179.5943 207.4861 92.6268 0.1373 2498 +2500 4 178.766 206.778 92.1998 0.1373 2499 +2501 4 178.0487 205.968 91.7445 0.1373 2500 +2502 4 177.2685 205.1913 91.3038 0.1373 2501 +2503 4 176.3213 204.5941 90.9462 0.1373 2502 +2504 4 175.2905 204.0965 90.6973 0.1373 2503 +2505 4 174.2575 203.608 90.5548 0.1373 2504 +2506 4 173.1627 203.3163 90.5136 0.1373 2505 +2507 4 172.0279 203.2167 90.5265 0.1373 2506 +2508 4 170.917 203.0406 90.58 0.1373 2507 +2509 4 170.1883 202.2684 90.5568 0.1373 2508 +2510 4 169.2411 201.7261 90.4151 0.1373 2509 +2511 4 168.4128 200.9676 90.1897 0.1373 2510 +2512 4 167.5377 200.4849 89.7523 0.1373 2511 +2513 4 166.5881 199.8911 89.2945 0.1373 2512 +2514 4 165.785 199.5582 88.6547 0.1373 2513 +2515 4 164.752 199.1052 88.1045 0.1373 2514 +2516 4 163.7613 198.5446 87.7285 0.1373 2515 +2517 4 162.9262 197.92 87.6288 0.1373 2516 +2518 4 161.8451 197.5471 87.5935 0.1373 2517 +2519 4 160.7995 197.0906 87.5717 0.1373 2518 +2520 4 159.8008 196.6193 87.4796 0.1374 2519 +2521 4 158.9828 196.0564 87.2351 0.1374 2520 +2522 4 158.3273 195.1698 87.0878 0.1375 2521 +2523 4 157.3755 194.6962 87.0988 0.1376 2522 +2524 4 156.4077 194.5052 87.2253 0.138 2523 +2525 4 155.322 194.4514 87.1923 0.1385 2524 +2526 4 154.2066 194.3176 87.0797 0.1397 2525 +2527 4 153.0775 194.2695 86.9142 0.1417 2526 +2528 4 151.993 194.0682 86.6998 0.1455 2527 +2529 4 151.0217 193.5751 86.525 0.1524 2528 +2530 4 150.0184 193.2296 86.4525 0.1658 2529 +2531 4 148.9797 192.8704 86.329 0.1878 2530 +2532 4 147.9386 192.4425 86.2445 0.2422 2531 +2533 4 147.052 191.7767 86.2512 0.2776 2532 +2534 4 146.194 191.1258 86.3839 0.3167 2533 +2535 4 145.2502 190.5046 86.5113 0.2906 2534 +2536 4 144.4334 189.7862 86.7412 0.2616 2535 +2537 4 143.6509 188.9659 86.9711 0.2291 2536 +2538 4 142.968 188.1674 87.2735 0.1798 2537 +2539 4 142.174 187.6732 87.2214 0.1504 2538 +2540 4 141.1604 187.3277 87.0341 0.138 2539 +2541 4 140.3002 186.5967 86.8902 0.1387 2540 +2542 4 139.5634 185.7215 86.7362 0.1398 2541 +2543 4 138.6848 185.0843 86.5606 0.142 2542 +2544 4 137.6529 184.6805 86.3621 0.146 2543 +2545 4 136.8327 183.9026 86.1991 0.1532 2544 +2546 4 135.9587 183.2425 86.0062 0.1677 2545 +2547 4 134.9085 182.8249 85.8085 0.1889 2546 +2548 4 133.943 182.2586 85.7212 0.2559 2547 +2549 4 133.1044 181.5082 85.6506 0.2461 2548 +2550 4 132.5244 180.5426 85.6528 0.1923 2549 +2551 4 131.7224 179.7384 85.6719 0.15 2550 +2552 4 130.7901 179.1126 85.6178 0.1373 2551 +2553 4 130.3485 178.0933 85.6016 0.1373 2552 +2554 4 130.5521 177.0214 85.6531 0.1373 2553 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2575 +2577 4 193.9091 211.2945 99.1925 0.1691 2576 +2578 4 193.3085 210.329 99.5728 0.2065 2577 +2579 4 192.3236 209.4824 100.9296 0.1373 2578 +2580 4 191.2791 209.4103 103.5684 0.1373 2579 +2581 4 190.5469 210.1334 104.629 0.1373 2580 +2582 4 190.2804 210.6196 106.1189 0.1373 2581 +2583 4 190.0367 211.0874 107.9417 0.1373 2582 +2584 4 189.7301 211.4501 109.9944 0.1372 2583 +2585 4 189.2714 211.5508 112.1772 0.1372 2584 +2586 4 188.6925 211.5508 114.375 0.1371 2585 +2587 4 187.9901 211.5508 116.5021 0.1368 2586 +2588 4 187.2019 211.5313 118.4868 0.1364 2587 +2589 4 186.4423 211.1755 120.2762 0.1356 2588 +2590 4 185.9412 210.3599 121.8694 0.1342 2589 +2591 4 185.5534 209.7192 123.3618 0.1313 2590 +2592 4 185.1804 209.0969 124.7512 0.1271 2591 +2593 4 184.2458 208.6359 125.8729 0.1144 2592 +2594 4 183.2299 208.3453 126.8277 0.1144 2593 +2595 4 192.8258 209.7581 99.752 0.1796 2578 +2596 4 192.3361 208.7846 99.8841 0.15 2595 +2597 4 192.2686 207.6566 99.99 0.1373 2596 +2598 4 192.3247 206.5137 100.1389 0.1373 2597 +2599 4 192.4471 205.3777 100.3696 0.1373 2598 +2600 4 192.359 204.2761 100.7667 0.1373 2599 +2601 4 192.2778 203.2602 101.3995 0.1373 2600 +2602 4 192.3098 202.186 102.1513 0.1373 2601 +2603 4 192.2698 201.0969 102.977 0.1373 2602 +2604 4 192.2584 199.9884 103.8246 0.1373 2603 +2605 4 192.2755 198.8821 104.6828 0.1373 2604 +2606 4 192.3899 197.809 105.5695 0.1373 2605 +2607 4 192.5363 196.7474 106.4846 0.1373 2606 +2608 4 192.9493 195.783 107.4492 0.1373 2607 +2609 4 193.4149 194.8175 108.4398 0.1373 2608 +2610 4 193.7902 193.8142 109.447 0.1373 2609 +2611 4 194.5921 193.2822 110.5042 0.1373 2610 +2612 4 195.5451 192.8464 111.5537 0.1373 2611 +2613 4 195.7132 192.0353 112.6952 0.1373 2612 +2614 4 196.2372 191.5777 114.8392 0.1144 2613 +2615 4 197.2839 191.4026 115.5706 0.1373 2614 +2616 4 198.4188 191.2791 115.8427 0.1373 2615 +2617 4 199.4198 190.7712 116.165 0.1373 2616 +2618 4 200.438 190.3055 116.5847 0.1373 2617 +2619 4 201.479 189.864 117.0691 0.1373 2618 +2620 4 202.5269 189.4167 117.5798 0.1373 2619 +2621 4 203.5199 189.0529 118.2255 0.1373 2620 +2622 4 204.5323 188.7165 118.9826 0.1373 2621 +2623 4 205.5139 188.4145 119.8422 0.1373 2622 +2624 4 206.1042 187.5394 120.7217 0.1372 2623 +2625 4 207.096 187.1275 121.5883 0.1372 2624 +2626 4 208.1176 186.8244 122.4325 0.1371 2625 +2627 4 209.2296 186.8232 123.1709 0.1368 2626 +2628 4 210.3427 186.8232 123.8129 0.1364 2627 +2629 4 211.473 186.8655 124.3388 0.1356 2628 +2630 4 212.6078 186.9285 124.7554 0.1342 2629 +2631 4 213.745 186.9937 125.0796 0.1313 2630 +2632 4 214.8844 187.06 125.3165 0.1271 2631 +2633 4 216.025 187.1264 125.4932 0.1144 2632 +2634 4 217.1278 186.8232 125.6951 0.1144 2633 +2635 4 195.5828 191.2539 113.6741 0.1373 2613 +2636 4 195.3998 190.1557 114.546 0.1373 2635 +2637 4 195.2167 189.0574 115.3284 0.1373 2636 +2638 4 195.0337 187.9592 116.0376 0.1373 2637 +2639 4 194.8507 186.861 116.6981 0.1373 2638 +2640 4 194.6676 185.7627 117.3264 0.1373 2639 +2641 4 194.4846 184.6645 117.9559 0.1373 2640 +2642 4 194.3015 183.5662 118.5786 0.1373 2641 +2643 4 194.1185 182.468 119.1935 0.1373 2642 +2644 4 193.9355 181.3698 119.7983 0.1373 2643 +2645 4 193.7524 180.2715 120.3902 0.1373 2644 +2646 4 193.5694 179.1733 120.9634 0.1373 2645 +2647 4 193.5751 178.9902 121.4461 0.1373 2646 +2648 4 193.6048 177.9423 121.989 0.1373 2647 +2649 4 193.5236 177.5328 122.4586 0.1373 2648 +2650 4 193.3657 176.4426 122.8864 0.1373 2649 +2651 4 193.1758 175.3466 123.1434 0.1373 2650 +2652 4 192.8635 174.2976 123.3145 0.1373 2651 +2653 4 192.6805 173.2188 123.5707 0.1373 2652 +2654 4 192.2423 172.2624 123.8356 0.1373 2653 +2655 4 191.572 171.3529 124.094 0.1373 2654 +2656 4 191.3043 170.3004 124.3925 0.1373 2655 +2657 4 190.929 169.2582 124.6448 0.1373 2656 +2658 4 190.2346 168.3659 124.7842 0.1373 2657 +2659 4 189.8525 167.3306 124.9181 0.1373 2658 +2660 4 189.3366 166.3273 124.9881 0.1373 2659 +2661 4 189.2771 165.2016 124.9814 0.1373 2660 +2662 4 189.054 164.0805 124.9976 0.1373 2661 +2663 4 188.9591 163.5863 125.06 0.1373 2662 +2664 4 188.7463 162.472 125.1905 0.1373 2663 +2665 4 188.5323 161.3589 125.3613 0.1373 2664 +2666 4 188.3196 160.2447 125.5626 0.1373 2665 +2667 4 188.2841 159.8934 125.7203 0.1373 2666 +2668 4 188.1708 158.7552 125.8264 0.1373 2667 +2669 4 188.0564 157.6169 125.8872 0.1373 2668 +2670 4 187.918 156.4809 125.9101 0.1373 2669 +2671 4 187.7007 155.3644 125.9076 0.1373 2670 +2672 4 187.2019 154.3348 125.8894 0.1372 2671 +2673 4 186.7088 153.3029 125.8642 0.1372 2672 +2674 4 186.1631 152.2984 125.8292 0.1371 2673 +2675 4 185.8131 151.2448 125.781 0.1368 2674 +2676 4 185.8359 150.1042 125.7136 0.1364 2675 +2677 4 185.8931 148.9625 125.6086 0.1356 2676 +2678 4 185.6769 147.8609 125.4733 0.1342 2677 +2679 4 185.2479 146.8004 125.316 0.1313 2678 +2680 4 185.1667 146.2547 125.0556 0.1271 2679 +2681 4 184.7285 145.4722 124.6904 0.1144 2680 +2682 4 184.1577 144.5284 123.7208 0.1144 2681 +2683 4 377.9078 551.0785 23.4343 0.232 1929 +2684 4 377.1047 550.3853 22.0767 0.1851 2683 +2685 4 376.3337 549.5707 21.5393 0.16 2684 +2686 4 375.3967 549.025 20.8831 0.1563 2685 +2687 4 374.4095 548.8512 20.0711 0.1703 2686 +2688 4 373.77 547.9268 19.298 0.2088 2687 +2689 4 374.0091 546.8572 18.4995 0.2207 2688 +2690 4 373.3822 546.3618 16.2865 0.268 2689 +2691 4 372.6157 546.6307 14.1202 0.182 2690 +2692 4 371.7645 547.134 13.2055 0.15 2691 +2693 4 370.9592 547.8193 12.2098 0.1582 2692 +2694 4 370.8219 548.7253 11.1138 0.189 2693 +2695 4 371.2017 549.6474 9.9869 0.171 2694 +2696 4 371.0438 550.6678 8.9299 0.1582 2695 +2697 4 370.1892 551.3966 8.0882 0.1497 2696 +2698 4 369.2077 551.9651 7.4061 0.1738 2697 +2699 4 368.6002 552.7579 6.7136 0.1398 2698 +2700 4 368.7512 553.8298 5.3788 0.1144 2699 +2701 4 374.3511 546.2348 17.788 0.1875 2689 +2702 4 375.1473 545.7955 16.9719 0.1652 2701 +2703 4 376.0019 545.9157 16.0166 0.1628 2702 +2704 4 376.9846 546.2726 15.1073 0.1973 2703 +2705 4 378.1 546.4419 14.3016 0.187 2704 +2706 4 378.9168 545.8619 13.496 0.1851 2705 +2707 4 379.395 544.8746 12.705 0.2124 2706 +2708 4 379.8263 543.8336 11.9711 0.228 2707 +2709 4 380.4143 542.995 11.1707 0.1985 2708 +2710 4 381.3055 542.7296 10.2422 0.1977 2709 +2711 4 382.3843 542.6633 9.3371 0.1638 2710 +2712 4 383.4516 542.3612 8.5221 0.144 2711 +2713 4 384.1312 541.6211 7.7053 0.1271 2712 +2714 4 385.1104 541.0765 7.0247 0.1144 2713 +2715 4 386.1206 540.5652 5.9738 0.1144 2714 +2716 2 397.9141 573.6977 22.4084 0.1952 1 +2717 2 398.1921 574.6358 21.5354 0.2565 2716 +2718 2 398.6886 575.6585 21.2051 0.3009 2717 +2719 2 399.5523 576.4078 20.9259 0.3761 2718 +2720 2 400.225 577.3322 20.6983 0.3205 2719 +2721 2 400.7947 578.3252 20.5194 0.3644 2720 +2722 2 401.425 579.2793 20.3815 0.3051 2721 +2723 2 402.0897 580.2071 20.2598 0.3051 2722 +2724 2 402.6926 581.1348 19.7456 0.4576 2723 +2725 3 398.9883 571.9142 23.7664 0.1374 1 +2726 3 399.8509 572.5731 24.9493 0.1374 2725 +2727 3 400.7135 573.2321 25.457 0.1376 2726 +2728 3 401.5692 573.9025 26.0442 0.1379 2727 +2729 3 402.2659 574.7044 26.6901 0.1383 2728 +2730 3 403.1822 575.3634 27.2517 0.1392 2729 +2731 3 404.0139 576.1287 27.7582 0.1408 2730 +2732 3 405.1362 576.3117 28.1296 0.1438 2731 +2733 3 405.9759 576.9958 28.3436 0.1499 2732 +2734 3 406.7961 577.6136 28.7277 0.1581 2733 +2735 3 407.6015 578.3721 29.1766 0.1885 2734 +2736 3 408.3256 579.2175 29.6696 0.1703 2735 +2737 3 408.8816 580.0927 30.2711 0.1563 2736 +2738 3 409.4342 580.834 30.8722 0.1494 2737 +2739 3 410.4718 581.0971 31.3099 0.1571 2738 +2740 3 411.4476 581.5501 31.7288 0.1868 2739 +2741 3 412.2862 582.3097 32.1059 0.1671 2740 +2742 3 413.0835 583.1071 32.4234 0.1503 2741 +2743 3 413.985 583.7718 32.6735 0.1379 2742 +2744 3 414.843 584.4593 32.9675 0.1383 2743 +2745 3 415.2983 585.4111 33.3466 0.1391 2744 +2746 3 415.5992 586.4876 33.6787 0.1407 2745 +2747 3 416.4721 587.1134 33.9819 0.1435 2746 +2748 3 417.258 587.881 34.1177 0.1494 2747 +2749 3 417.8975 588.8225 34.2544 0.1571 2748 +2750 3 418.5713 589.7423 34.3994 0.1867 2749 +2751 3 419.3458 590.5797 34.5038 0.1669 2750 +2752 3 420.0059 591.472 34.7077 0.15 2751 +2753 3 420.9337 592.1115 34.9765 0.1373 2752 +2754 3 421.826 592.7785 35.3175 0.1373 2753 +2755 3 422.7446 593.4523 35.644 0.1373 2754 +2756 3 423.6678 594.1215 35.9386 0.1373 2755 +2757 3 424.2272 595.0436 36.1584 0.1373 2756 +2758 3 424.8507 595.8307 36.3947 0.1373 2757 +2759 3 425.743 596.3603 36.7802 0.1373 2758 +2760 3 426.6182 596.9323 37.2669 0.1373 2759 +2761 3 427.6352 597.3957 37.6737 0.1373 2760 +2762 3 428.6705 597.8796 37.9854 0.1373 2761 +2763 3 429.7001 598.3784 38.2231 0.1373 2762 +2764 3 430.708 598.9195 38.3953 0.1373 2763 +2765 3 431.4768 599.7203 38.5932 0.1373 2764 +2766 3 431.8783 600.7579 38.7316 0.1373 2765 +2767 3 432.6871 601.498 38.8245 0.1373 2766 +2768 3 433.3209 602.4201 39.046 0.1374 2767 +2769 3 433.7064 603.3994 39.468 0.1375 2768 +2770 3 434.2086 604.1704 40.1044 0.1376 2769 +2771 3 434.5026 605.16 40.7896 0.1379 2770 +2772 3 434.982 605.6645 41.4669 0.1384 2771 +2773 3 434.9854 606.797 41.9591 0.1395 2772 +2774 3 435.2748 607.8964 42.2598 0.1413 2773 +2775 3 435.7771 608.9157 42.4276 0.1445 2774 +2776 3 436.4085 609.863 42.5099 0.1512 2775 +2777 3 437.0011 610.8319 42.5225 0.1605 2776 +2778 3 437.5663 611.8181 42.56 0.193 2777 +2779 3 438.0113 612.8259 42.6406 0.1792 2778 +2780 3 438.1978 613.931 42.7602 0.17 2779 +2781 3 438.4243 615.0304 42.9159 0.1873 2780 +2782 3 438.3934 616.1538 43.0377 0.1679 2781 +2783 3 438.0262 617.2292 43.1332 0.1518 2782 +2784 3 437.747 618.3228 43.204 0.1407 2783 +2785 3 437.421 619.4028 43.251 0.1435 2784 +2786 3 437.0069 620.4198 43.1945 0.1494 2785 +2787 3 436.8581 621.4082 43.2188 0.1571 2786 +2788 3 436.6065 622.3795 43.433 0.1867 2787 +2789 3 436.3788 623.464 43.6528 0.1669 2788 +2790 3 436.4234 624.4696 43.6517 0.15 2789 +2791 3 435.6878 624.9363 43.4193 0.1373 2790 +2792 3 435.2565 625.8538 43.3432 0.1373 2791 +2793 3 434.3265 626.2954 43.4476 0.1373 2792 +2794 3 433.6744 627.1053 43.5929 0.1373 2793 +2795 3 433.4685 628.183 43.647 0.1373 2794 +2796 3 433.2397 629.2103 43.5632 0.1373 2795 +2797 3 433.3758 630.2765 43.5408 0.1373 2796 +2798 3 433.0349 631.3198 43.5943 0.1373 2797 +2799 3 432.8141 632.2957 43.5806 0.1373 2798 +2800 3 432.4709 633.1479 43.4865 0.1373 2799 +2801 3 432.0694 634.2142 43.4134 0.1373 2800 +2802 3 431.5465 635.2049 43.3989 0.1373 2801 +2803 3 431.4379 636.3328 43.3163 0.1373 2802 +2804 3 431.399 637.4608 43.2144 0.1373 2803 +2805 3 431.1107 638.5064 43.0469 0.1373 2804 +2806 3 430.7583 639.5669 42.959 0.1373 2805 +2807 3 430.7091 640.6892 43.0024 0.1373 2806 +2808 3 430.5192 641.7851 43.1547 0.1373 2807 +2809 3 430.2001 642.8273 43.4171 0.1373 2808 +2810 3 429.7139 643.8409 43.6453 0.1373 2809 +2811 3 429.1007 644.7836 43.7676 0.1373 2810 +2812 3 428.2839 645.3899 43.8038 0.1373 2811 +2813 3 427.459 646.0854 43.748 0.1374 2812 +2814 3 427.1353 647.1265 43.7259 0.1375 2813 +2815 3 427.093 648.2545 43.7606 0.1376 2814 +2816 3 427.1227 649.3779 43.7559 0.138 2815 +2817 3 427.2714 650.4956 43.715 0.1384 2816 +2818 3 427.1639 651.6064 43.6458 0.1398 2817 +2819 3 426.5598 652.4644 43.7248 0.1398 2818 +2820 3 426.1446 653.3567 44.0003 0.1525 2819 +2821 3 425.9444 654.4538 44.3369 0.1144 2820 diff --git a/docs/tutorial/05_opt_extracellular/components/synaptic_models/AMPA_ExcToExc.json b/docs/tutorial/05_opt_extracellular/components/synaptic_models/AMPA_ExcToExc.json new file mode 100644 index 00000000..c7585403 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/synaptic_models/AMPA_ExcToExc.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 1.0, + "tau2": 3.0, + "erev": 0.0 +} diff --git a/docs/tutorial/05_opt_extracellular/components/synaptic_models/AMPA_ExcToInh.json b/docs/tutorial/05_opt_extracellular/components/synaptic_models/AMPA_ExcToInh.json new file mode 100644 index 00000000..43887996 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/synaptic_models/AMPA_ExcToInh.json @@ -0,0 +1,6 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 0.1, + "tau2": 0.5, + "erev": 0.0 +} diff --git a/docs/tutorial/05_opt_extracellular/components/synaptic_models/GABA_InhToExc.json b/docs/tutorial/05_opt_extracellular/components/synaptic_models/GABA_InhToExc.json new file mode 100644 index 00000000..702ce9b4 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/synaptic_models/GABA_InhToExc.json @@ -0,0 +1,7 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 2.7, + "tau2": 15.0, + "erev": -70.0 +} + diff --git a/docs/tutorial/05_opt_extracellular/components/synaptic_models/GABA_InhToInh.json b/docs/tutorial/05_opt_extracellular/components/synaptic_models/GABA_InhToInh.json new file mode 100644 index 00000000..ed4130ab --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/components/synaptic_models/GABA_InhToInh.json @@ -0,0 +1,7 @@ +{ + "level_of_detail": "exp2syn", + "tau1": 0.2, + "tau2": 8.0, + "erev": -70.0 +} + diff --git a/docs/tutorial/05_opt_extracellular/config.lfp.json b/docs/tutorial/05_opt_extracellular/config.lfp.json new file mode 100755 index 00000000..a914a382 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/config.lfp.json @@ -0,0 +1,74 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$OUTPUT_DIR": "$BASE_DIR/output_lfp", + "$NETWORK_DIR": "$BASE_DIR/network_lfp", + "$COMPONENTS_DIR": "$BASE_DIR/components" + }, + "target_simulator": "NEURON", + "run": { + "tstart": 0.0, + "tstop": 2000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15.0, + "nsteps_block": 5000 + }, + "conditions": { + "celsius": 34.0, + "v_init": -80.0 + }, + "inputs": { + "virt_exc_spikes": { + "input_type": "spikes", + "module": "sonata", + "input_file": "$BASE_DIR/inputs/virt_spikes.h5", + "node_set": "thalamus" + } + }, + "components": { + "filter_models_dir": "$COMPONENTS_DIR/filter_models", + "biophysical_neuron_models_dir": "$COMPONENTS_DIR/biophysical_neuron_models", + "mechanisms_dir": "$COMPONENTS_DIR/mechanisms", + "synaptic_models_dir": "$COMPONENTS_DIR/synaptic_models", + "morphologies_dir": "$COMPONENTS_DIR/morphologies", + "point_neuron_models_dir": "$COMPONENTS_DIR/point_neuron_models" + }, + "output": { + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv" + }, + "reports": { + "cortical_electrode": { + "cells": "all", + "variable_name": "v", + "module": "extracellular", + "electrode_positions": "$COMPONENTS_DIR/electrodes/linear_electrode.csv", + "electrode_channels": "all" + } + }, + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/cortcol_nodes.h5", + "node_types_file": "$NETWORK_DIR/cortcol_node_types.csv" + }, + { + "nodes_file": "$NETWORK_DIR/thalamus_nodes.h5", + "node_types_file": "$NETWORK_DIR/thalamus_node_types.csv" + } + ], + "edges": [ + { + "edge_types_file": "$NETWORK_DIR/cortcol_cortcol_edge_types.csv", + "edges_file": "$NETWORK_DIR/cortcol_cortcol_edges.h5" + }, + { + "edge_types_file": "$NETWORK_DIR/thalamus_cortcol_edge_types.csv", + "edges_file": "$NETWORK_DIR/thalamus_cortcol_edges.h5" + } + ] + } +} \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/icsd_scripts/demo_icsd.py b/docs/tutorial/05_opt_extracellular/icsd_scripts/demo_icsd.py new file mode 100755 index 00000000..ba4e7f2e --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/icsd_scripts/demo_icsd.py @@ -0,0 +1,234 @@ +# -*- coding: utf-8 -*- +'''iCSD toolbox demonstration script''' +import matplotlib.pyplot as plt +import numpy as np +import icsd +from scipy import io +import neo +import quantities as pq + +#patch quantities with the SI unit Siemens if it does not exist +for symbol, prefix, definition, u_symbol in zip( + ['siemens', 'S', 'mS', 'uS', 'nS', 'pS'], + ['', '', 'milli', 'micro', 'nano', 'pico'], + [pq.A/pq.V, pq.A/pq.V, 'S', 'mS', 'uS', 'nS'], + [None, None, None, None, u'µS', None]): + if type(definition) is str: + definition = lastdefinition / 1000 + if not hasattr(pq, symbol): + setattr(pq, symbol, pq.UnitQuantity( + prefix + 'siemens', + definition, + symbol=symbol, + u_symbol=u_symbol)) + lastdefinition = definition + +#loading test data +test_data = io.loadmat('test_data.mat') + +#prepare lfp data for use, by changing the units to SI and append quantities, +#along with electrode geometry, conductivities and assumed source geometry +lfp_data = test_data['pot1'] * 1E-6 * pq.V # [uV] -> [V] +z_data = np.linspace(100E-6, 2300E-6, 23) * pq.m # [m] +diam = 500E-6 * pq.m # [m] +h = 100E-6 * pq.m # [m] +sigma = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] +sigma_top = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] + +# Input dictionaries for each method +delta_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'diam' : diam, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. +} +step_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'diam' : diam, + 'h' : h, # source thickness + 'sigma' : sigma, + 'sigma_top' : sigma, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} +spline_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'diam' : diam, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : 201, # Spatial CSD upsampling to N steps + 'tol' : 1E-12, + 'f_type' : 'gaussian', + 'f_order' : (20, 5), +} +std_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} + + +#Create the different CSD-method class instances. We use the class methods +#get_csd() and filter_csd() below to get the raw and spatially filtered +#versions of the current-source density estimates. +csd_dict = dict( + delta_icsd = icsd.DeltaiCSD(**delta_input), + step_icsd = icsd.StepiCSD(**step_input), + spline_icsd = icsd.SplineiCSD(**spline_input), + std_csd = icsd.StandardCSD(**std_input), +) + +#plot +for method, csd_obj in list(csd_dict.items()): + fig, axes = plt.subplots(3,1, figsize=(8,8)) + + #plot LFP signal + ax = axes[0] + im = ax.imshow(np.array(lfp_data), origin='upper', vmin=-abs(lfp_data).max(), \ + vmax=abs(lfp_data).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + cb = plt.colorbar(im, ax=ax) + cb.set_label('LFP (%s)' % lfp_data.dimensionality.string) + ax.set_xticklabels([]) + ax.set_title('LFP') + ax.set_ylabel('ch #') + + #plot raw csd estimate + csd = csd_obj.get_csd() + ax = axes[1] + im = ax.imshow(np.array(csd), origin='upper', vmin=-abs(csd).max(), \ + vmax=abs(csd).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(csd_obj.name) + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_xticklabels([]) + ax.set_ylabel('ch #') + + #plot spatially filtered csd estimate + ax = axes[2] + csd = csd_obj.filter_csd(csd) + im = ax.imshow(np.array(csd), origin='upper', vmin=-abs(csd).max(), \ + vmax=abs(csd).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(csd_obj.name + ', filtered') + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_ylabel('ch #') + ax.set_xlabel('timestep') + + +# ############################################################################ # +# Demonstrate icsd.estimate_csd() function on neo.AnalogSignalArray object # +# ############################################################################ # +lfp_data = neo.AnalogSignalArray(lfp_data.T, sampling_rate=2.*pq.kHz) + +# Input dictionaries for each method +delta_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'delta', + 'diam' : diam, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. +} +step_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'step', + 'diam' : diam, + 'h' : h, # source thickness + 'sigma' : sigma, + 'sigma_top' : sigma, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} +spline_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'spline', + 'diam' : diam, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : 201, # Spatial CSD upsampling to N steps + 'tol' : 1E-12, + 'f_type' : 'gaussian', + 'f_order' : (20, 5), +} +std_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'standard', + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} + +#compute CSD and filtered CSD estimates. Note that the returned argument of the +#function is a tuple of neo.AnalogSignalArray objects (csd, csd_filtered) +csd_dict = dict( + delta_icsd = icsd.estimate_csd(**delta_input), + step_icsd = icsd.estimate_csd(**step_input), + spline_icsd = icsd.estimate_csd(**spline_input), + std_csd = icsd.estimate_csd(**std_input), +) + +#plot +for method, csd_obj in list(csd_dict.items()): + fig, axes = plt.subplots(3,1, figsize=(8,8)) + + #plot LFP signal + ax = axes[0] + im = ax.imshow(lfp_data.magnitude.T, origin='upper', + vmin=-abs(lfp_data.magnitude).max(), + vmax=abs(lfp_data.magnitude).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + cb = plt.colorbar(im, ax=ax) + cb.set_label('LFP (%s)' % lfp_data.dimensionality.string) + ax.set_xticklabels([]) + ax.set_title('LFP') + ax.set_ylabel('ch #') + + #plot raw csd estimate + csd = csd_obj[0] + ax = axes[1] + im = ax.imshow(csd.magnitude.T, origin='upper', + vmin=-abs(csd.magnitude).max(), + vmax=abs(csd.magnitude).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(method) + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_xticklabels([]) + ax.set_ylabel('ch #') + + #plot spatially filtered csd estimate + ax = axes[2] + csd = csd_obj[1] + im = ax.imshow(csd.magnitude.T, origin='upper', + vmin=-abs(csd.magnitude).max(), + vmax=abs(csd.magnitude).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(method + ', filtered') + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_ylabel('ch #') + ax.set_xlabel('timestep') + + + +plt.show() diff --git a/docs/tutorial/05_opt_extracellular/icsd_scripts/icsd.py b/docs/tutorial/05_opt_extracellular/icsd_scripts/icsd.py new file mode 100755 index 00000000..6d634e80 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/icsd_scripts/icsd.py @@ -0,0 +1,1331 @@ +# -*- coding: utf-8 -*- +""" +py-iCSD toolbox! +Translation of the core functionality of the CSDplotter MATLAB package +to python. + +The methods were originally developed by Klas H. Pettersen, as described in: +Klas H. Pettersen, Anna Devor, Istvan Ulbert, Anders M. Dale, Gaute T. Einevoll, +Current-source density estimation based on inversion of electrostatic forward +solution: Effects of finite extent of neuronal activity and conductivity +discontinuities, Journal of Neuroscience Methods, Volume 154, Issues 1-2, +30 June 2006, Pages 116-133, ISSN 0165-0270, +http://dx.doi.org/10.1016/j.jneumeth.2005.12.005. +(http://www.sciencedirect.com/science/article/pii/S0165027005004541) + +The method themselves are implemented as callable subclasses of the base +CSD class object, which sets some common attributes, +and a basic function for calculating the iCSD, and a generic spatial filter +implementation. + +The raw- and filtered CSD estimates are returned as Quantity arrays. + +Requires pylab environment to work, i.e numpy+scipy+matplotlib, with the +addition of quantities (http://pythonhosted.org/quantities) and +neo (https://pythonhosted.org/neo)- + +Original implementation from CSDplotter-0.1.1 +(http://software.incf.org/software/csdplotter) by Klas. H. Pettersen 2005. + +Written by: +- Espen.Hagen@umb.no, 2010, +- e.hagen@fz-juelich.de, 2015-2016 + + +Example +------- +#!/usr/env/python + +import matplotlib.pyplot as plt +import numpy as np +import icsd +from scipy import io +import quantities as pq + +#loading test data +test_data = io.loadmat('test_data.mat') + +#prepare lfp data for use, by changing the units to SI and append quantities, +#along with electrode geometry and conductivities +lfp_data = test_data['pot1'] * 1E-6 * pq.V # [uV] -> [V] +z_data = np.linspace(100E-6, 2300E-6, 23) * pq.m # [m] +diam = 500E-6 * pq.m # [m] +h = 100E-6 * pq.m # [m] +sigma = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] +sigma_top = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] + +# Input dictionaries for each method +delta_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'diam' : diam, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. +} +step_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'diam' : diam, + 'h' : h, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} +spline_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'diam' : diam, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : 201, # Spatial CSD upsampling to N steps + 'tol' : 1E-12, + 'f_type' : 'gaussian', + 'f_order' : (20, 5), +} +std_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} + + +#Create the different CSD-method class instances. We use the class methods +#get_csd() and filter_csd() below to get the raw and spatially filtered +#versions of the current-source density estimates. +csd_dict = dict( + delta_icsd = icsd.DeltaiCSD(**delta_input), + step_icsd = icsd.StepiCSD(**step_input), + spline_icsd = icsd.SplineiCSD(**spline_input), + std_csd = icsd.StandardCSD(**std_input), +) + +#plot +for method, csd_obj in csd_dict.items(): + fig, axes = plt.subplots(3,1, figsize=(8,8)) + + #plot LFP signal + ax = axes[0] + im = ax.imshow(np.array(lfp_data), origin='upper', vmin=-abs(lfp_data).max(), \ + vmax=abs(lfp_data).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + cb = plt.colorbar(im, ax=ax) + cb.set_label('LFP (%s)' % lfp_data.dimensionality.string) + ax.set_xticklabels([]) + ax.set_title('LFP') + ax.set_ylabel('ch #') + + #plot raw csd estimate + csd = csd_obj.get_csd() + ax = axes[1] + im = ax.imshow(np.array(csd), origin='upper', vmin=-abs(csd).max(), \ + vmax=abs(csd).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(csd_obj.name) + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_xticklabels([]) + ax.set_ylabel('ch #') + + #plot spatially filtered csd estimate + ax = axes[2] + csd = csd_obj.filter_csd(csd) + im = ax.imshow(np.array(csd), origin='upper', vmin=-abs(csd).max(), \ + vmax=abs(csd).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(csd_obj.name + ', filtered') + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_ylabel('ch #') + ax.set_xlabel('timestep') + +plt.show() + + +Example +------- +#!/usr/env/python + +import matplotlib.pyplot as plt +import numpy as np +import icsd +from scipy import io +import neo +import quantities as pq + +#loading test data +test_data = io.loadmat('test_data.mat') + +#prepare lfp data for use, by changing the units to SI and append quantities, +#along with electrode geometry and conductivities +lfp_data = neo.AnalogSignalArray(test_data['pot1'].T * 1E-6 * pq.V, + sampling_rate=2.*pq.kHz) +z_data = np.linspace(100E-6, 2300E-6, 23) * pq.m # [m] +diam = 500E-6 * pq.m # [m] +h = 100E-6 * pq.m # [m] +sigma = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] +sigma_top = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] + +# Input dictionaries for each method +delta_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'delta', + 'diam' : diam, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. +} +step_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'step', + 'diam' : diam, + 'h' : h, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} +spline_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'spline', + 'diam' : diam, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : 201, # Spatial CSD upsampling to N steps + 'tol' : 1E-12, + 'f_type' : 'gaussian', + 'f_order' : (20, 5), +} +std_input = { + 'lfp' : lfp_data, + 'coord_electrode' : z_data, + 'method' : 'standard', + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), +} + +#compute CSD and filtered CSD estimates. Note that the returned argument of the +#function is a tuple of neo.AnalogSignalArray objects (csd, csd_filtered) +csd_dict = dict( + delta_icsd = icsd.estimate_csd(**delta_input), + step_icsd = icsd.estimate_csd(**step_input), + spline_icsd = icsd.estimate_csd(**spline_input), + std_csd = icsd.estimate_csd(**std_input), +) + +#plot +for method, csd_obj in csd_dict.items(): + fig, axes = plt.subplots(3,1, figsize=(8,8)) + + #plot LFP signal + ax = axes[0] + im = ax.imshow(lfp_data.magnitude.T, origin='upper', + vmin=-abs(lfp_data.magnitude).max(), + vmax=abs(lfp_data.magnitude).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + cb = plt.colorbar(im, ax=ax) + cb.set_label('LFP (%s)' % lfp_data.dimensionality.string) + ax.set_xticklabels([]) + ax.set_title('LFP') + ax.set_ylabel('ch #') + + #plot raw csd estimate + csd = csd_obj[0] + ax = axes[1] + im = ax.imshow(csd.magnitude.T, origin='upper', + vmin=-abs(csd.magnitude).max(), + vmax=abs(csd.magnitude).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(method) + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_xticklabels([]) + ax.set_ylabel('ch #') + + #plot spatially filtered csd estimate + ax = axes[2] + csd = csd_obj[1] + im = ax.imshow(csd.magnitude.T, origin='upper', + vmin=-abs(csd.magnitude).max(), + vmax=abs(csd.magnitude).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(method + ', filtered') + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_ylabel('ch #') + ax.set_xlabel('timestep') + +plt.show() + + + +""" + +import numpy as np +import scipy.integrate as si +import scipy.signal as ss +import quantities as pq +import neo + + +# patch quantities with the SI unit Siemens if it does not exist +for symbol, prefix, definition, u_symbol in zip( + ["siemens", "S", "mS", "uS", "nS", "pS"], + ["", "", "milli", "micro", "nano", "pico"], + [pq.A / pq.V, pq.A / pq.V, "S", "mS", "uS", "nS"], + [None, None, None, None, "µS", None], +): + if type(definition) is str: + definition = lastdefinition / 1000 + if not hasattr(pq, symbol): + setattr( + pq, + symbol, + pq.UnitQuantity( + prefix + "siemens", definition, symbol=symbol, u_symbol=u_symbol + ), + ) + lastdefinition = definition + + +class CSD(object): + """Base iCSD class""" + + def __init__(self, lfp, f_type="gaussian", f_order=(3, 1)): + """Initialize parent class iCSD + + Parameters + ---------- + lfp : np.ndarray * quantity.Quantity + LFP signal of shape (# channels, # time steps) + f_type : str + type of spatial filter, must be a scipy.signal filter design method + f_order : list + settings for spatial filter, arg passed to filter design function + """ + self.name = "CSD estimate parent class" + self.lfp = lfp + self.f_matrix = np.eye(lfp.shape[0]) * pq.m**3 / pq.S + self.f_type = f_type + self.f_order = f_order + + def get_csd( + self, + ): + """ + Perform the CSD estimate from the LFP and forward matrix F, i.e as + CSD=F**-1*LFP + + Arguments + --------- + + Returns + ------- + csd : np.ndarray * quantity.Quantity + Array with the csd estimate + """ + csd = np.linalg.solve(self.f_matrix, self.lfp) + + return csd * (self.f_matrix.units**-1 * self.lfp.units).simplified + + def filter_csd(self, csd, filterfunction="convolve"): + """ + Spatial filtering of the CSD estimate, using an N-point filter + + Arguments + --------- + csd : np.ndarrray * quantity.Quantity + Array with the csd estimate + filterfunction : str + 'filtfilt' or 'convolve'. Apply spatial filter using + scipy.signal.filtfilt or scipy.signal.convolve. + """ + if self.f_type == "gaussian": + try: + assert len(self.f_order) == 2 + except AssertionError as ae: + raise ae("filter order f_order must be a tuple of length 2") + else: + try: + assert self.f_order > 0 and isinstance(self.f_order, int) + except AssertionError as ae: + raise ae("Filter order must be int > 0!") + try: + assert filterfunction in ["filtfilt", "convolve"] + except AssertionError as ae: + raise ae("{} not equal to 'filtfilt' or 'convolve'".format(filterfunction)) + + if self.f_type == "boxcar": + num = ss.windows.boxcar(self.f_order) + denom = np.array([num.sum()]) + elif self.f_type == "hamming": + num = ss.windows.hamming(self.f_order) + denom = np.array([num.sum()]) + elif self.f_type == "triangular": + num = ss.windows.triang(self.f_order) + denom = np.array([num.sum()]) + elif self.f_type == "gaussian": + num = ss.windows.gaussian(self.f_order[0], self.f_order[1]) + denom = np.array([num.sum()]) + elif self.f_type == "identity": + num = np.array([1.0]) + denom = np.array([1.0]) + else: + print("%s Wrong filter type!" % self.f_type) + raise + + num_string = "[ " + for i in num: + num_string = num_string + "%.3f " % i + num_string = num_string + "]" + denom_string = "[ " + for i in denom: + denom_string = denom_string + "%.3f " % i + denom_string = denom_string + "]" + + print( + ( + "discrete filter coefficients: \nb = {}, \na = {}".format( + num_string, denom_string + ) + ) + ) + + if filterfunction == "filtfilt": + return ss.filtfilt(num, denom, csd, axis=0) * csd.units + elif filterfunction == "convolve": + csdf = csd / csd.units + for i in range(csdf.shape[1]): + csdf[:, i] = ss.convolve(csdf[:, i], num / denom.sum(), "same") + return csdf * csd.units + + +class StandardCSD(CSD): + """ + Standard CSD method with and without Vaknin electrodes + """ + + def __init__( + self, + lfp, + coord_electrode=np.linspace(0, 1400e-6, 15) * pq.m, + sigma=0.3 * pq.S / pq.m, + vaknin_el=True, + f_type="gaussian", + f_order=(3, 1), + ): + """ + Initialize standard CSD method class with and without Vaknin electrodes. + + Parameters + ---------- + lfp : np.ndarray * quantity.Quantity + LFP signal of shape (# channels, # time steps) in units of V + coord_electrode : np.ndarray * quantity.Quantity + depth of evenly spaced electrode contact points of shape + (# contacts, ) in units of m, must be monotonously increasing + sigma : float * quantity.Quantity + conductivity of tissue in units of S/m or 1/(ohm*m) + vaknin_el : bool + flag for using method of Vaknin to endpoint electrodes + f_type : str + type of spatial filter, must be a scipy.signal filter design method + f_order : list + settings for spatial filter, arg passed to filter design function + """ + CSD.__init__(self, lfp, f_type, f_order) + + self.name = "Standard CSD method" + self.coord_electrode = coord_electrode + self.sigma = sigma + self.vaknin_el = vaknin_el + + try: + assert np.all(np.diff(np.diff(coord_electrode))) == 0 + except AssertionError as ae: + print("coord_electrode not monotonously varying") + raise ae + + if vaknin_el: + # extend array of lfps by duplicating potential at endpoint contacts + if lfp.ndim == 1: + self.lfp = np.empty((lfp.shape[0] + 2,)) * lfp.units + else: + self.lfp = np.empty((lfp.shape[0] + 2, lfp.shape[1])) * lfp.units + self.lfp[0,] = lfp[0,] + self.lfp[1:-1,] = lfp + self.lfp[-1,] = lfp[-1,] + else: + self.lfp = lfp + + self.f_inv_matrix = self.get_f_inv_matrix() + + def get_f_inv_matrix(self): + """Calculate the inverse F-matrix for the standard CSD method""" + h_val = abs(np.diff(self.coord_electrode)[0]) + + f_inv = -np.eye(self.lfp.shape[0]) + + # Inner matrix elements is just the discrete laplacian coefficients + for j in range(1, f_inv.shape[0] - 1): + f_inv[j, j - 1 : j + 2] = np.array([1.0, -2.0, 1.0]) + + return f_inv * -self.sigma / h_val + + def get_csd(self): + """ + Perform the iCSD calculation, i.e: iCSD=F_inv*LFP + + Returns + ------- + csd : np.ndarray * quantity.Quantity + Array with the csd estimate + """ + csd = np.dot(self.f_inv_matrix, self.lfp)[1:-1,] + # `np.dot()` does not return correct units, so the units of `csd` must + # be assigned manually + csd_units = (self.f_inv_matrix.units * self.lfp.units).simplified + csd = csd.magnitude * csd_units + + return csd + + +class DeltaiCSD(CSD): + """ + delta-iCSD method + """ + + def __init__( + self, + lfp, + coord_electrode=np.linspace(0, 1400e-6, 15) * pq.m, + diam=500e-6 * pq.m, + sigma=0.3 * pq.S / pq.m, + sigma_top=0.3 * pq.S / pq.m, + f_type="gaussian", + f_order=(3, 1), + ): + """ + Initialize the delta-iCSD method class object + + Parameters + ---------- + lfp : np.ndarray * quantity.Quantity + LFP signal of shape (# channels, # time steps) in units of V + coord_electrode : np.ndarray * quantity.Quantity + depth of evenly spaced electrode contact points of shape + (# contacts, ) in units of m + diam : float * quantity.Quantity + diamater of the assumed circular planar current sources centered + at each contact + sigma : float * quantity.Quantity + conductivity of tissue in units of S/m or 1/(ohm*m) + sigma_top : float * quantity.Quantity + conductivity on top of tissue in units of S/m or 1/(ohm*m) + f_type : str + type of spatial filter, must be a scipy.signal filter design method + f_order : list + settings for spatial filter, arg passed to filter design function + + """ + CSD.__init__(self, lfp) + + try: + assert diam.units == coord_electrode.units + except AssertionError as ae: + print( + "units of coord_electrode ({}) and diam ({}) differ".format( + coord_electrode.units, diam.units + ) + ) + raise ae + + try: + assert np.all(np.diff(coord_electrode) > 0) + except AssertionError as ae: + print("values of coord_electrode not continously increasing") + raise ae + + try: + assert diam.size == 1 or diam.size == coord_electrode.size + if diam.size == coord_electrode.size: + assert np.all(diam > 0 * diam.units) + else: + assert diam > 0 * diam.units + except AssertionError as ae: + print("diam must be positive scalar or of same shape as coord_electrode") + raise ae + if diam.size == 1: + diam = np.ones(coord_electrode.size) * diam + + self.name = "delta-iCSD method" + self.coord_electrode = coord_electrode + self.diam = diam + self.sigma = sigma + self.sigma_top = sigma_top + self.f_type = f_type + self.f_order = f_order + + # initialize F- and iCSD-matrices + self.f_matrix = np.empty((self.coord_electrode.size, self.coord_electrode.size)) + + self.f_matrix = self.get_f_matrix() + + def get_f_matrix(self): + """Calculate the F-matrix""" + f_matrix = ( + np.empty((self.coord_electrode.size, self.coord_electrode.size)) + * self.coord_electrode.units + ) + for j in range(self.coord_electrode.size): + for i in range(self.coord_electrode.size): + f_matrix[j, i] = ( + np.sqrt( + (self.coord_electrode[j] - self.coord_electrode[i]) ** 2 + + (self.diam[j] / 2) ** 2 + ) + - abs(self.coord_electrode[j] - self.coord_electrode[i]) + ) + (self.sigma - self.sigma_top) / (self.sigma + self.sigma_top) * ( + np.sqrt( + (self.coord_electrode[j] + self.coord_electrode[i]) ** 2 + + (self.diam[j] / 2) ** 2 + ) + - abs(self.coord_electrode[j] + self.coord_electrode[i]) + ) + + f_matrix /= 2 * self.sigma + return f_matrix + + +class StepiCSD(CSD): + """step-iCSD method""" + + def __init__( + self, + lfp, + coord_electrode=np.linspace(0, 1400e-6, 15) * pq.m, + diam=500e-6 * pq.m, + h=np.ones(15) * 100e-6 * pq.m, + sigma=0.3 * pq.S / pq.m, + sigma_top=0.3 * pq.S / pq.m, + tol=1e-6, + f_type="gaussian", + f_order=(3, 1), + ): + """ + Initializing step-iCSD method class object + + Parameters + ---------- + lfp : np.ndarray * quantity.Quantity + LFP signal of shape (# channels, # time steps) in units of V + coord_electrode : np.ndarray * quantity.Quantity + depth of evenly spaced electrode contact points of shape + (# contacts, ) in units of m + diam : float or np.ndarray * quantity.Quantity + diameter(s) of the assumed circular planar current sources centered + at each contact + h : float or np.ndarray * quantity.Quantity + assumed thickness of the source cylinders at all or each contact + sigma : float * quantity.Quantity + conductivity of tissue in units of S/m or 1/(ohm*m) + sigma_top : float * quantity.Quantity + conductivity on top of tissue in units of S/m or 1/(ohm*m) + tol : float + tolerance of numerical integration + f_type : str + type of spatial filter, must be a scipy.signal filter design method + f_order : list + settings for spatial filter, arg passed to filter design function + + """ + CSD.__init__(self, lfp, f_type, f_order) + + try: + assert diam.units == coord_electrode.units + except AssertionError as ae: + print( + "units of coord_electrode ({}) and diam ({}) differ".format( + coord_electrode.units, diam.units + ) + ) + raise ae + try: + assert np.all(np.diff(coord_electrode) > 0) + except AssertionError as ae: + print("values of coord_electrode not continously increasing") + raise ae + + try: + assert diam.size == 1 or diam.size == coord_electrode.size + if diam.size == coord_electrode.size: + assert np.all(diam > 0 * diam.units) + else: + assert diam > 0 * diam.units + except AssertionError as ae: + print("diam must be positive scalar or of same shape as coord_electrode") + raise ae + if diam.size == 1: + diam = np.ones(coord_electrode.size) * diam + + try: + assert h.size == 1 or h.size == coord_electrode.size + if h.size == coord_electrode.size: + assert np.all(h > 0 * h.units) + except AssertionError as ae: + print("h must be scalar or of same shape as coord_electrode") + raise ae + if h.size == 1: + h = np.ones(coord_electrode.size) * h + + self.name = "step-iCSD method" + self.coord_electrode = coord_electrode + self.diam = diam + self.h = h + self.sigma = sigma + self.sigma_top = sigma_top + self.tol = tol + + # compute forward-solution matrix + self.f_matrix = self.get_f_matrix() + + def get_f_matrix(self): + """Calculate F-matrix for step iCSD method""" + el_len = self.coord_electrode.size + f_matrix = np.zeros((el_len, el_len)) + for j in range(el_len): + for i in range(el_len): + lower_int = self.coord_electrode[i] - self.h[j] / 2 + if lower_int < 0: + lower_int = self.h[j].units + upper_int = self.coord_electrode[i] + self.h[j] / 2 + + # components of f_matrix object + f_cyl0 = si.quad( + self._f_cylinder, + a=lower_int, + b=upper_int, + args=( + float(self.coord_electrode[j]), + float(self.diam[j]), + float(self.sigma), + ), + epsabs=self.tol, + )[0] + f_cyl1 = si.quad( + self._f_cylinder, + a=lower_int, + b=upper_int, + args=( + -float(self.coord_electrode[j]), + float(self.diam[j]), + float(self.sigma), + ), + epsabs=self.tol, + )[0] + + # method of images coefficient + mom = (self.sigma - self.sigma_top) / (self.sigma + self.sigma_top) + + f_matrix[j, i] = f_cyl0 + mom * f_cyl1 + + # assume si.quad trash the units + return f_matrix * self.h.units**2 / self.sigma.units + + def _f_cylinder(self, zeta, z_val, diam, sigma): + """function used by class method""" + f_cyl = ( + 1.0 + / (2.0 * sigma) + * (np.sqrt((diam / 2) ** 2 + ((z_val - zeta)) ** 2) - abs(z_val - zeta)) + ) + return f_cyl + + +class SplineiCSD(CSD): + """spline iCSD method""" + + def __init__( + self, + lfp, + coord_electrode=np.linspace(0, 1400e-6, 15) * pq.m, + diam=500e-6 * pq.m, + sigma=0.3 * pq.S / pq.m, + sigma_top=0.3 * pq.S / pq.m, + tol=1e-6, + f_type="gaussian", + f_order=(3, 1), + num_steps=200, + ): + """ + Initializing spline-iCSD method class object + + Parameters + ---------- + lfp : np.ndarray * quantity.Quantity + LFP signal of shape (# channels, # time steps) in units of V + coord_electrode : np.ndarray * quantity.Quantity + depth of evenly spaced electrode contact points of shape + (# contacts, ) in units of m + diam : float * quantity.Quantity + diamater of the assumed circular planar current sources centered + at each contact + sigma : float * quantity.Quantity + conductivity of tissue in units of S/m or 1/(ohm*m) + sigma_top : float * quantity.Quantity + conductivity on top of tissue in units of S/m or 1/(ohm*m) + tol : float + tolerance of numerical integration + f_type : str + type of spatial filter, must be a scipy.signal filter design method + f_order : list + settings for spatial filter, arg passed to filter design function + num_steps : int + number of data points for the spatially upsampled LFP/CSD data + + """ + CSD.__init__(self, lfp, f_type, f_order) + + try: + assert diam.units == coord_electrode.units + except AssertionError as ae: + print( + "units of coord_electrode ({}) and diam ({}) differ".format( + coord_electrode.units, diam.units + ) + ) + raise + try: + assert np.all(np.diff(coord_electrode) > 0) + except AssertionError as ae: + print("values of coord_electrode not continously increasing") + raise ae + + try: + assert diam.size == 1 or diam.size == coord_electrode.size + if diam.size == coord_electrode.size: + assert np.all(diam > 0 * diam.units) + except AssertionError as ae: + print("diam must be scalar or of same shape as coord_electrode") + raise ae + if diam.size == 1: + diam = np.ones(coord_electrode.size) * diam + + self.name = "spline-iCSD method" + self.coord_electrode = coord_electrode + self.diam = diam + self.sigma = sigma + self.sigma_top = sigma_top + self.tol = tol + self.num_steps = num_steps + + # compute stuff + self.f_matrix = self.get_f_matrix() + + def get_f_matrix(self): + """Calculate the F-matrix for cubic spline iCSD method""" + el_len = self.coord_electrode.size + z_js = np.zeros(el_len + 1) + z_js[:-1] = np.array(self.coord_electrode) + z_js[-1] = z_js[-2] + float(np.diff(self.coord_electrode).mean()) + + # Define integration matrixes + f_mat0 = np.zeros((el_len, el_len + 1)) + f_mat1 = np.zeros((el_len, el_len + 1)) + f_mat2 = np.zeros((el_len, el_len + 1)) + f_mat3 = np.zeros((el_len, el_len + 1)) + + # Calc. elements + for j in range(el_len): + for i in range(el_len): + f_mat0[j, i] = si.quad( + self._f_mat0, + a=z_js[i], + b=z_js[i + 1], + args=(z_js[j + 1], float(self.sigma), float(self.diam[j])), + epsabs=self.tol, + )[0] + f_mat1[j, i] = si.quad( + self._f_mat1, + a=z_js[i], + b=z_js[i + 1], + args=(z_js[j + 1], z_js[i], float(self.sigma), float(self.diam[j])), + epsabs=self.tol, + )[0] + f_mat2[j, i] = si.quad( + self._f_mat2, + a=z_js[i], + b=z_js[i + 1], + args=(z_js[j + 1], z_js[i], float(self.sigma), float(self.diam[j])), + epsabs=self.tol, + )[0] + f_mat3[j, i] = si.quad( + self._f_mat3, + a=z_js[i], + b=z_js[i + 1], + args=(z_js[j + 1], z_js[i], float(self.sigma), float(self.diam[j])), + epsabs=self.tol, + )[0] + + # image technique if conductivity not constant: + if self.sigma != self.sigma_top: + f_mat0[j, i] = ( + f_mat0[j, i] + + (self.sigma - self.sigma_top) + / (self.sigma + self.sigma_top) + * si.quad( + self._f_mat0, + a=z_js[i], + b=z_js[i + 1], + args=(-z_js[j + 1], float(self.sigma), float(self.diam[j])), + epsabs=self.tol, + )[0] + ) + f_mat1[j, i] = ( + f_mat1[j, i] + + (self.sigma - self.sigma_top) + / (self.sigma + self.sigma_top) + * si.quad( + self.f_mat1, + a=z_js[i], + b=z_js[i + 1], + args=( + -z_js[j + 1], + z_js[i], + float(self.sigma), + float(self.diam[j]), + ), + epsabs=self.tol, + )[0] + ) + f_mat2[j, i] = ( + f_mat2[j, i] + + (self.sigma - self.sigma_top) + / (self.sigma + self.sigma_top) + * si.quad( + self._f_mat2, + a=z_js[i], + b=z_js[i + 1], + args=( + -z_js[j + 1], + z_js[i], + float(self.sigma), + float(self.diam[j]), + ), + epsabs=self.tol, + )[0] + ) + f_mat3[j, i] = ( + f_mat3[j, i] + + (self.sigma - self.sigma_top) + / (self.sigma + self.sigma_top) + * si.quad( + self._f_mat3, + a=z_js[i], + b=z_js[i + 1], + args=( + -z_js[j + 1], + z_js[i], + float(self.sigma), + float(self.diam[j]), + ), + epsabs=self.tol, + )[0] + ) + + e_mat0, e_mat1, e_mat2, e_mat3 = self._calc_e_matrices() + + # Calculate the F-matrix + f_matrix = np.eye(el_len + 2) + f_matrix[1:-1, :] = ( + np.dot(f_mat0, e_mat0) + + np.dot(f_mat1, e_mat1) + + np.dot(f_mat2, e_mat2) + + np.dot(f_mat3, e_mat3) + ) + + return f_matrix * self.coord_electrode.units**2 / self.sigma.units + + def get_csd(self): + """ + Calculate the iCSD using the spline iCSD method + + Returns + ------- + csd : np.ndarray * quantity.Quantity + Array with csd estimate + + + """ + e_mat = self._calc_e_matrices() + + el_len = self.coord_electrode.size + + # padding the lfp with zeros on top/bottom + if self.lfp.ndim == 1: + cs_lfp = np.r_[[0], np.asarray(self.lfp), [0]].reshape(1, -1).T + csd = np.zeros(self.num_steps) + else: + cs_lfp = np.vstack( + ( + np.zeros(self.lfp.shape[1]), + np.asarray(self.lfp), + np.zeros(self.lfp.shape[1]), + ) + ) + csd = np.zeros((self.num_steps, self.lfp.shape[1])) + cs_lfp *= self.lfp.units + + # CSD coefficients + csd_coeff = np.linalg.solve(self.f_matrix, cs_lfp) + + # The cubic spline polynomial coefficients + a_mat0 = np.dot(e_mat[0], csd_coeff) + a_mat1 = np.dot(e_mat[1], csd_coeff) + a_mat2 = np.dot(e_mat[2], csd_coeff) + a_mat3 = np.dot(e_mat[3], csd_coeff) + + # Extend electrode coordinates in both end by min contact interdistance + h = np.diff(self.coord_electrode).min() + z_js = np.zeros(el_len + 2) + z_js[0] = self.coord_electrode[0] - h + z_js[1:-1] = self.coord_electrode + z_js[-1] = self.coord_electrode[-1] + h + + # create high res spatial grid + out_zs = np.linspace(z_js[1], z_js[-2], self.num_steps) + + # Calculate iCSD estimate on grid from polynomial coefficients. + i = 0 + for j in range(self.num_steps): + if out_zs[j] >= z_js[i + 1]: + i += 1 + csd[j,] = ( + a_mat0[i, :] + + a_mat1[i, :] * (out_zs[j] - z_js[i]) + + a_mat2[i, :] * (out_zs[j] - z_js[i]) ** 2 + + a_mat3[i, :] * (out_zs[j] - z_js[i]) ** 3 + ) + + csd_unit = (self.f_matrix.units**-1 * self.lfp.units).simplified + + return csd * csd_unit + + def _f_mat0(self, zeta, z_val, sigma, diam): + """0'th order potential function""" + return ( + 1.0 + / (2.0 * sigma) + * (np.sqrt((diam / 2) ** 2 + ((z_val - zeta)) ** 2) - abs(z_val - zeta)) + ) + + def _f_mat1(self, zeta, z_val, zi_val, sigma, diam): + """1'th order potential function""" + return (zeta - zi_val) * self._f_mat0(zeta, z_val, sigma, diam) + + def _f_mat2(self, zeta, z_val, zi_val, sigma, diam): + """2'nd order potential function""" + return (zeta - zi_val) ** 2 * self._f_mat0(zeta, z_val, sigma, diam) + + def _f_mat3(self, zeta, z_val, zi_val, sigma, diam): + """3'rd order potential function""" + return (zeta - zi_val) ** 3 * self._f_mat0(zeta, z_val, sigma, diam) + + def _calc_k_matrix(self): + """Calculate the K-matrix used by to calculate E-matrices""" + el_len = self.coord_electrode.size + h = float(np.diff(self.coord_electrode).min()) + + c_jm1 = np.eye(el_len + 2, k=0) / h + c_jm1[0, 0] = 0 + + c_j0 = np.eye(el_len + 2) / h + c_j0[-1, -1] = 0 + + c_jall = c_j0 + c_jall[0, 0] = 1 + c_jall[-1, -1] = 1 + + tjp1 = np.eye(el_len + 2, k=1) + tjm1 = np.eye(el_len + 2, k=-1) + + tj0 = np.eye(el_len + 2) + tj0[0, 0] = 0 + tj0[-1, -1] = 0 + + # Defining K-matrix used to calculate e_mat1-3 + return np.dot( + np.linalg.inv( + np.dot(c_jm1, tjm1) + + 2 * np.dot(c_jm1, tj0) + + 2 * c_jall + + np.dot(c_j0, tjp1) + ), + 3 + * ( + np.dot(np.dot(c_jm1, c_jm1), tj0) + - np.dot(np.dot(c_jm1, c_jm1), tjm1) + + np.dot(np.dot(c_j0, c_j0), tjp1) + - np.dot(np.dot(c_j0, c_j0), tj0) + ), + ) + + def _calc_e_matrices(self): + """Calculate the E-matrices used by cubic spline iCSD method""" + el_len = self.coord_electrode.size + ## expanding electrode grid + h = float(np.diff(self.coord_electrode).min()) + + ## Define transformation matrices + c_mat3 = np.eye(el_len + 1) / h + + # Get K-matrix + k_matrix = self._calc_k_matrix() + + # Define matrixes for C to A transformation: + tja = np.eye(el_len + 2)[:-1,] + tjp1a = np.eye(el_len + 2, k=1)[:-1,] + + # Define spline coefficients + e_mat0 = tja + e_mat1 = np.dot(tja, k_matrix) + e_mat2 = 3 * np.dot(c_mat3**2, (tjp1a - tja)) - np.dot( + np.dot(c_mat3, (tjp1a + 2 * tja)), k_matrix + ) + e_mat3 = 2 * np.dot(c_mat3**3, (tja - tjp1a)) + np.dot( + np.dot(c_mat3**2, (tjp1a + tja)), k_matrix + ) + + return e_mat0, e_mat1, e_mat2, e_mat3 + + +def estimate_csd( + lfp, + coord_electrode, + sigma, + method="standard", + diam=None, + h=None, + sigma_top=None, + tol=1e-6, + num_steps=200, + f_type="identity", + f_order=None, +): + """ + Estimates current source density (CSD) from local field potential (LFP) + recordings from multiple depths of the cortex. + + Parameters + ---------- + lfp : neo.AnalogSignalArray + LFP signals from which CSD is estimated. + coord_electrode : Quantity array + Depth of evenly spaced electrode contact points. + sigma : Quantity float + Conductivity of tissue. + method : string + CSD estimation method, either of 'standard': the standard + double-derivative method, 'delta': delta-iCSD method, 'step': + step-iCSD method, 'spline': spline-iCSD method. Default is 'standard' + diam : Quantity float + Diamater of the assumed circular planar current sources centered at + each contact, required by iCSD methods (= 'delta', 'step', + 'spline'). Default is `None`. + h : float or np.ndarray * quantity.Quantity + assumed thickness of the source cylinders at all or each contact + sigma_top : Quantity float + Conductivity on top of tissue. When set to `None`, the same value as + sigma: is used. Default is `None`. + tol : float + Tolerance of numerical integration, required by step- and + spline-iCSD methods. Default is 1E-6. + num_steps : int + Number of data points for the spatially upsampled LFP/CSD data, + required by spline-iCSD method. Default is 200. + f_type : string + Type of spatial + used for smoothing of the result, either of + 'boxcar' (uses `scipy.signal.baxcar()`), 'hamming' ( + `scipy.signal.hamming()`), 'triangular' (`scipy.signal.tri()`), + 'gaussian' (`scipy.signal.gaussian`), 'identity' (no smoothing is + applied). Default is 'identity'. + f_order : float tuple + Parameters to be passed to the scipy.signal function associated with + the specified filter type. + + + Returns + ------- + tuple : (csd, csd_filtered) + csd : neo.AnalogSignalArray + Estimated CSD + csd_filtered : neo.AnalogSignalArray + Estimated CSD, spatially filtered + + + Example + ------- + import numpy as np + import matplotlib.pyplot as plt + from scipy import io + import quantities as pq + import neo + + import icsd + + + #loading test data + test_data = io.loadmat('test_data.mat') + + #prepare lfp data for use, by changing the units to SI and append + #quantities, along with electrode geometry and conductivities + lfp_data = test_data['pot1'] * 1E-3 * pq.V # [mV] -> [V] + z_data = np.linspace(100E-6, 2300E-6, 23) * pq.m # [m] + diam = 500E-6 * pq.m # [m] + sigma = 0.3 * pq.S / pq.m # [S/m] or [1/(ohm*m)] + sigma_top = 0. * pq.S / pq.m # [S/m] or [1/(ohm*m)] + + lfp = neo.AnalogSignalArray(lfp_data.T, sampling_rate=2.0*pq.kHz) + + # Input dictionaries for each method + params = {} + params['delta'] = { + 'method': 'delta', + 'lfp' : lfp, + 'coord_electrode' : z_data, + 'diam' : diam, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma, # conductivity on top of cortex + } + params['step'] = { + 'method': 'step', + 'lfp' : lfp, + 'coord_electrode' : z_data, + 'diam' : diam, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'tol' : 1E-12, # Tolerance in numerical integration + } + params['spline'] = { + 'method': 'spline', + 'lfp' : lfp, + 'coord_electrode' : z_data, + 'diam' : diam, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : 201, # Spatial CSD upsampling to N steps + 'tol' : 1E-12, + } + params['standard'] = { + 'method': 'standard', + 'lfp' : lfp, + 'coord_electrode' : z_data, + 'sigma' : sigma, + } + + #plot LFP signal + fig, axes = plt.subplots(len(params)+1, 1, figsize=(6, 8)) + ax = axes[0] + im = ax.imshow(lfp.magnitude.T, origin='upper', vmin=-abs(lfp).max(), + vmax=abs(lfp).max(), cmap='jet_r', interpolation='nearest') + ax.axis(ax.axis('tight')) + cb = plt.colorbar(im, ax=ax) + cb.set_label('LFP (%s)' % lfp_data.dimensionality.string) + ax.set_xticklabels([]) + ax.set_title('LFP') + ax.set_ylabel('ch #') + i_ax = 1 + for method, param in params.items(): + ax = axes[i_ax] + i_ax += 1 + csd = icsd.estimate_csd(**param) + im = ax.imshow(csd.magnitude.T, origin='upper', vmin=-abs(csd).max(), + vmax=abs(csd).max(), cmap='jet_r', + interpolation='nearest') + ax.axis(ax.axis('tight')) + ax.set_title(method) + cb = plt.colorbar(im, ax=ax) + cb.set_label('CSD (%s)' % csd.dimensionality.string) + ax.set_xticklabels([]) + ax.set_ylabel('ch #') + + plt.show() + """ + + supported_methods = ("standard", "delta", "step", "spline") + icsd_methods = ("delta", "step", "spline") + + if method not in supported_methods: + print( + "Pamareter `method` must be either of {}".format( + ", ".join(supported_methods) + ) + ) + raise ValueError + elif method in icsd_methods and diam is None: + print( + "Parameter `diam` must be specified for iCSD methods: {}".format( + ", ".join(icsd_methods) + ) + ) + raise ValueError + + if not isinstance(lfp, neo.AnalogSignalArray): + print("Parameter `lfp` must be neo.AnalogSignalArray") + raise TypeError + + if f_type is not "identity" and f_order is None: + print("The order of {} filter must be specified".format(f_type)) + raise ValueError + + lfp_pqarr = lfp.magnitude.T * lfp.units + if sigma_top is None: + sigma_top = sigma + + arg_dict = { + "lfp": lfp_pqarr, + "coord_electrode": coord_electrode, + "sigma": sigma, + "f_type": f_type, + "f_order": f_order, + } + if method == "standard": + csd_estimator = StandardCSD(**arg_dict) + else: + arg_dict["diam"] = diam + arg_dict["sigma_top"] = sigma_top + if method == "delta": + csd_estimator = DeltaiCSD(**arg_dict) + else: + arg_dict["tol"] = tol + if method == "step": + arg_dict["h"] = h + csd_estimator = StepiCSD(**arg_dict) + else: + arg_dict["num_steps"] = num_steps + csd_estimator = SplineiCSD(**arg_dict) + csd_pqarr = csd_estimator.get_csd() + csd_pqarr_filtered = csd_estimator.filter_csd(csd_pqarr) + csd = neo.AnalogSignalArray( + csd_pqarr.T, t_start=lfp.t_start, sampling_rate=lfp.sampling_rate + ) + csd_filtered = neo.AnalogSignalArray( + csd_pqarr_filtered.T, t_start=lfp.t_start, sampling_rate=lfp.sampling_rate + ) + + return csd, csd_filtered diff --git a/docs/tutorial/05_opt_extracellular/icsd_scripts/test_icsd.py b/docs/tutorial/05_opt_extracellular/icsd_scripts/test_icsd.py new file mode 100755 index 00000000..ac57a9f2 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/icsd_scripts/test_icsd.py @@ -0,0 +1,1243 @@ +# -*- coding: utf-8 -*- +'''icsd testing suite''' + +import os +import numpy as np +import numpy.testing as nt +import quantities as pq +import scipy.integrate as si +from scipy.interpolate import interp1d +import icsd +import unittest + +#patch quantities with the SI unit Siemens if it does not exist +for symbol, prefix, definition, u_symbol in zip( + ['siemens', 'S', 'mS', 'uS', 'nS', 'pS'], + ['', '', 'milli', 'micro', 'nano', 'pico'], + [pq.A/pq.V, pq.A/pq.V, 'S', 'mS', 'uS', 'nS'], + [None, None, None, None, u'µS', None]): + if type(definition) is str: + definition = lastdefinition / 1000 + if not hasattr(pq, symbol): + setattr(pq, symbol, pq.UnitQuantity( + prefix + 'siemens', + definition, + symbol=symbol, + u_symbol=u_symbol)) + lastdefinition = definition + + +def potential_of_plane(z_j, z_i=0.*pq.m, + C_i=1*pq.A/pq.m**2, + sigma=0.3*pq.S/pq.m): + ''' + Return potential of infinite horizontal plane with constant + current source density at a vertical offset z_j. + + Arguments + --------- + z_j : float*pq.m + distance perpendicular to source layer + z_i : float*pq.m + z-position of source layer + C_i : float*pq.A/pq.m**2 + current source density on circular disk in units of charge per area + sigma : float*pq.S/pq.m + conductivity of medium in units of S/m + + Notes + ----- + The potential is 0 at the plane, as the potential goes to infinity for + large distances + + ''' + try: + assert(z_j.units == z_i.units) + except AssertionError as ae: + print('units of z_j ({}) and z_i ({}) not equal'.format(z_j.units, + z_i.units)) + raise ae + + return -C_i/(2*sigma)*abs(z_j-z_i).simplified + + +def potential_of_disk(z_j, + z_i=0.*pq.m, + C_i=1*pq.A/pq.m**2, + R_i=1E-3*pq.m, + sigma=0.3*pq.S/pq.m): + ''' + Return potential of circular disk in horizontal plane with constant + current source density at a vertical offset z_j. + + Arguments + --------- + z_j : float*pq.m + distance perpendicular to center of disk + z_i : float*pq.m + z_j-position of source disk + C_i : float*pq.A/pq.m**2 + current source density on circular disk in units of charge per area + R_i : float*pq.m + radius of disk source + sigma : float*pq.S/pq.m + conductivity of medium in units of S/m + ''' + try: + assert(z_j.units == z_i.units == R_i.units) + except AssertionError as ae: + print('units of z_j ({}), z_i ({}) and R_i ({}) not equal'.format( + z_j.units, z_i.units, R_i.units)) + raise ae + + return C_i/(2*sigma)*(np.sqrt((z_j-z_i)**2 + R_i**2) - abs(z_j-z_i)).simplified + + +def potential_of_cylinder(z_j, + z_i=0.*pq.m, + C_i=1*pq.A/pq.m**3, + R_i=1E-3*pq.m, + h_i=0.1*pq.m, + sigma=0.3*pq.S/pq.m, + ): + ''' + Return potential of cylinder in horizontal plane with constant homogeneous + current source density at a vertical offset z_j. + + + Arguments + --------- + z_j : float*pq.m + distance perpendicular to center of disk + z_i : float*pq.m + z-position of center of source cylinder + h_i : float*pq.m + thickness of cylinder + C_i : float*pq.A/pq.m**3 + current source density on circular disk in units of charge per area + R_i : float*pq.m + radius of disk source + sigma : float*pq.S/pq.m + conductivity of medium in units of S/m + + Notes + ----- + Sympy can't deal with eq. 11 in Pettersen et al 2006, J neurosci Meth, + so we numerically evaluate it in this function. + + Tested with + + >>>from sympy import * + >>>C_i, z_i, h, z_j, z_j, sigma, R = symbols('C_i z_i h z z_j sigma R') + >>>C_i*integrate(1/(2*sigma)*(sqrt((z-z_j)**2 + R**2) - abs(z-z_j)), (z, z_i-h/2, z_i+h/2)) + + + ''' + try: + assert(z_j.units == z_i.units == R_i.units == h_i.units) + except AssertionError as ae: + print('units of z_j ({}), z_i ({}), R_i ({}) and h ({}) not equal'.format( + z_j.units, z_i.units, R_i.units, h_i.units)) + raise ae + + #speed up tests by stripping units + _sigma = float(sigma) + _R_i = float(R_i) + _z_i = float(z_i) + _z_j = float(z_j) + + #evaluate integrand using quad + def integrand(z): + return 1/(2*_sigma)*(np.sqrt((z-_z_j)**2 + _R_i**2) - abs(z-_z_j)) + + phi_j, abserr = C_i*si.quad(integrand, z_i-h_i/2, z_i+h_i/2) + + return (phi_j * z_i.units**2 / sigma.units) + + + +def get_lfp_of_planes(z_j=np.arange(21)*1E-4*pq.m, + z_i=np.array([8E-4, 10E-4, 12E-4])*pq.m, + C_i=np.array([-.5, 1., -.5])*pq.A/pq.m**2, + sigma=0.3*pq.S/pq.m, + plot=True): + ''' + Compute the lfp of spatially separated planes with given current source + density + ''' + phi_j = np.zeros(z_j.size)*pq.V + for i, (zi, Ci) in enumerate(zip(z_i, C_i)): + for j, zj in enumerate(z_j): + phi_j[j] += potential_of_plane(zj, zi, Ci, sigma) + + #test plot + if plot: + import matplotlib.pyplot as plt + plt.figure() + plt.subplot(121) + ax = plt.gca() + ax.plot(np.zeros(z_j.size), z_j, 'r-o') + for i, C in enumerate(C_i): + ax.plot((0, C), (z_i[i], z_i[i]), 'r-o') + ax.set_ylim(z_j.min(), z_j.max()) + ax.set_ylabel('z_j ({})'.format(z_j.units)) + ax.set_xlabel('C_i ({})'.format(C_i.units)) + ax.set_title('planar CSD') + + plt.subplot(122) + ax = plt.gca() + ax.plot(phi_j, z_j, 'r-o') + ax.set_ylim(z_j.min(), z_j.max()) + ax.set_xlabel('phi_j ({})'.format(phi_j.units)) + ax.set_title('LFP') + + return phi_j, C_i + + +def get_lfp_of_disks(z_j=np.arange(21)*1E-4*pq.m, + z_i=np.array([8E-4, 10E-4, 12E-4])*pq.m, + C_i=np.array([-.5, 1., -.5])*pq.A/pq.m**2, + R_i = np.array([1, 1, 1])*1E-3*pq.m, + sigma=0.3*pq.S/pq.m, + plot=True): + ''' + Compute the lfp of spatially separated disks with a given + current source density + ''' + phi_j = np.zeros(z_j.size)*pq.V + for i, (zi, Ci, Ri) in enumerate(zip(z_i, C_i, R_i)): + for j, zj in enumerate(z_j): + phi_j[j] += potential_of_disk(zj, zi, Ci, Ri, sigma) + + #test plot + if plot: + import matplotlib.pyplot as plt + plt.figure() + plt.subplot(121) + ax = plt.gca() + ax.plot(np.zeros(z_j.size), z_j, 'r-o') + for i, C in enumerate(C_i): + ax.plot((0, C), (z_i[i], z_i[i]), 'r-o') + ax.set_ylim(z_j.min(), z_j.max()) + ax.set_ylabel('z_j ({})'.format(z_j.units)) + ax.set_xlabel('C_i ({})'.format(C_i.units)) + ax.set_title('disk CSD\nR={}'.format(R_i)) + + plt.subplot(122) + ax = plt.gca() + ax.plot(phi_j, z_j, 'r-o') + ax.set_ylim(z_j.min(), z_j.max()) + ax.set_xlabel('phi_j ({})'.format(phi_j.units)) + ax.set_title('LFP') + + return phi_j, C_i + + +def get_lfp_of_cylinders(z_j=np.arange(21)*1E-4*pq.m, + z_i=np.array([8E-4, 10E-4, 12E-4])*pq.m, + C_i=np.array([-.5, 1., -.5])*pq.A/pq.m**3, + R_i = np.array([1, 1, 1])*1E-3*pq.m, + h_i=np.array([1, 1, 1])*1E-4*pq.m, + sigma=0.3*pq.S/pq.m, + plot=True): + ''' + Compute the lfp of spatially separated disks with a given + current source density + ''' + phi_j = np.zeros(z_j.size)*pq.V + for i, (zi, Ci, Ri, hi) in enumerate(zip(z_i, C_i, R_i, h_i)): + for j, zj in enumerate(z_j): + phi_j[j] += potential_of_cylinder(zj, zi, Ci, Ri, hi, sigma) + + #test plot + if plot: + import matplotlib.pyplot as plt + plt.figure() + plt.subplot(121) + ax = plt.gca() + ax.plot(np.zeros(z_j.size), z_j, 'r-o') + ax.barh(np.asarray(z_i-h_i/2), + np.asarray(C_i), + np.asarray(h_i), color='r') + ax.set_ylim(z_j.min(), z_j.max()) + ax.set_ylabel('z_j ({})'.format(z_j.units)) + ax.set_xlabel('C_i ({})'.format(C_i.units)) + ax.set_title('cylinder CSD\nR={}'.format(R_i)) + + plt.subplot(122) + ax = plt.gca() + ax.plot(phi_j, z_j, 'r-o') + ax.set_ylim(z_j.min(), z_j.max()) + ax.set_xlabel('phi_j ({})'.format(phi_j.units)) + ax.set_title('LFP') + + return phi_j, C_i + + + + + + +class TestICSD(unittest.TestCase): + ''' + Set of test functions for each CSD estimation method comparing + estimate to LFPs calculated with known ground truth CSD + ''' + + def test_StandardCSD_00(self): + '''test using standard SI units''' + #set some parameters for ground truth csd and csd estimates. + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #uniform conductivity + sigma = 0.3*pq.S/pq.m + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_planes(z_j, z_i, C_i, sigma, plot) + std_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + std_csd = icsd.StandardCSD(**std_input) + csd = std_csd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StandardCSD_01(self): + '''test using non-standard SI units 1''' + #set some parameters for ground truth csd and csd estimates. + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*1E3*pq.A/pq.m**2 + + #uniform conductivity + sigma = 0.3*pq.S/pq.m + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_planes(z_j, z_i, C_i, sigma, plot) + std_input = { + 'lfp' : phi_j*1E3*pq.mV/pq.V, + 'coord_electrode' : z_j, + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + std_csd = icsd.StandardCSD(**std_input) + csd = std_csd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StandardCSD_02(self): + '''test using non-standard SI units 2''' + #set some parameters for ground truth csd and csd estimates. + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #uniform conductivity + sigma = 0.3*pq.S/pq.m + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_planes(z_j, z_i, C_i, sigma, plot) + std_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j*1E3*pq.mm/pq.m, + 'sigma' : sigma, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + std_csd = icsd.StandardCSD(**std_input) + csd = std_csd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StandardCSD_03(self): + '''test using non-standard SI units 3''' + #set some parameters for ground truth csd and csd estimates. + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #uniform conductivity + sigma = 0.3*pq.mS/pq.m + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_planes(z_j, z_i, C_i, sigma, plot) + std_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'sigma' : sigma*1E3*pq.mS/pq.S, + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + std_csd = icsd.StandardCSD(**std_input) + csd = std_csd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_DeltaiCSD_00(self): + '''test using standard SI units''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_disks(z_j, z_i, C_i, R_i, sigma, + plot) + delta_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i.mean()*2, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma_top, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. + } + + delta_icsd = icsd.DeltaiCSD(**delta_input) + csd = delta_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_DeltaiCSD_01(self): + '''test using non-standard SI units 1''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_disks(z_j, z_i, C_i, R_i, sigma, + plot) + delta_input = { + 'lfp' : phi_j*1E3*pq.mV/pq.V, + 'coord_electrode' : z_j, + 'diam' : R_i.mean()*2, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma_top, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. + } + + delta_icsd = icsd.DeltaiCSD(**delta_input) + csd = delta_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_DeltaiCSD_02(self): + '''test using non-standard SI units 2''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_disks(z_j, z_i, C_i, R_i, sigma, + plot) + delta_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j*1E3*pq.mm/pq.m, + 'diam' : R_i.mean()*2*1E3*pq.mm/pq.m, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma_top, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. + } + + delta_icsd = icsd.DeltaiCSD(**delta_input) + csd = delta_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_DeltaiCSD_03(self): + '''test using non-standard SI units 3''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_disks(z_j, z_i, C_i, R_i, sigma, + plot) + delta_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i.mean()*2, # source diameter + 'sigma' : sigma*1E3*pq.mS/pq.S, # extracellular conductivity + 'sigma_top' : sigma_top*1E3*pq.mS/pq.S, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. + } + + delta_icsd = icsd.DeltaiCSD(**delta_input) + csd = delta_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_DeltaiCSD_04(self): + '''test non-continous z_j array''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**2 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**2 + + #source radius (delta, step) + R_i = np.ones(z_j.size)*1E-3*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_disks(z_j, z_i, C_i, R_i, sigma, + plot) + inds = np.delete(np.arange(21), 5) + delta_input = { + 'lfp' : phi_j[inds], + 'coord_electrode' : z_j[inds], + 'diam' : R_i[inds]*2, # source diameter + 'sigma' : sigma, # extracellular conductivity + 'sigma_top' : sigma_top, # conductivity on top of cortex + 'f_type' : 'gaussian', # gaussian filter + 'f_order' : (3, 1), # 3-point filter, sigma = 1. + } + + delta_icsd = icsd.DeltaiCSD(**delta_input) + csd = delta_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i[inds], csd) + + + def test_StepiCSD_units_00(self): + '''test using standard SI units''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i, C_i, R_i, h_i, + sigma, plot) + + step_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i.mean()*2, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'h' : h_i, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + step_icsd = icsd.StepiCSD(**step_input) + csd = step_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StepiCSD_01(self): + '''test using non-standard SI units 1''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i, C_i, R_i, h_i, + sigma, plot) + + step_input = { + 'lfp' : phi_j*1E3*pq.mV/pq.V, + 'coord_electrode' : z_j, + 'diam' : R_i.mean()*2, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'h' : h_i, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + step_icsd = icsd.StepiCSD(**step_input) + csd = step_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StepiCSD_02(self): + '''test using non-standard SI units 2''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i, C_i, R_i, h_i, + sigma, plot) + + step_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j*1E3*pq.mm/pq.m, + 'diam' : R_i.mean()*2*1E3*pq.mm/pq.m, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'h' : h_i*1E3*pq.mm/pq.m, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + step_icsd = icsd.StepiCSD(**step_input) + csd = step_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StepiCSD_03(self): + '''test using non-standard SI units 3''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i, C_i, R_i, h_i, + sigma, plot) + + step_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i.mean()*2, + 'sigma' : sigma*1E3*pq.mS/pq.S, + 'sigma_top' : sigma*1E3*pq.mS/pq.S, + 'h' : h_i, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + step_icsd = icsd.StepiCSD(**step_input) + csd = step_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd) + + + def test_StepiCSD_units_04(self): + '''test non-continous z_j array''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i, C_i, R_i, h_i, + sigma, plot) + inds = np.delete(np.arange(21), 5) + step_input = { + 'lfp' : phi_j[inds], + 'coord_electrode' : z_j[inds], + 'diam' : R_i[inds]*2, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'h' : h_i[inds], + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + step_icsd = icsd.StepiCSD(**step_input) + csd = step_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i[inds], csd) + + + def test_SplineiCSD_00(self): + '''test using standard SI units''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #construct interpolators, spline method assume underlying source + #pattern generating LFPs that are cubic spline interpolates between + #contacts so we generate CSD data relying on the same assumption + f_C = interp1d(z_i, C_i, kind='cubic') + f_R = interp1d(z_i, R_i) + num_steps = 201 + z_i_i = np.linspace(float(z_i[0]), float(z_i[-1]), num_steps)*z_i.units + C_i_i = f_C(np.asarray(z_i_i))*C_i.units + R_i_i = f_R(z_i_i)*R_i.units + + h_i_i = np.ones(z_i_i.size)*np.diff(z_i_i).min() + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i_i, C_i_i, R_i_i, h_i_i, + sigma, plot) + + spline_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i*2, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : num_steps, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + spline_icsd = icsd.SplineiCSD(**spline_input) + csd = spline_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd, decimal=3) + + + def test_SplineiCSD_01(self): + '''test using standard SI units, deep electrode coordinates''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(10, 31)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #construct interpolators, spline method assume underlying source + #pattern generating LFPs that are cubic spline interpolates between + #contacts so we generate CSD data relying on the same assumption + f_C = interp1d(z_i, C_i, kind='cubic') + f_R = interp1d(z_i, R_i) + num_steps = 201 + z_i_i = np.linspace(float(z_i[0]), float(z_i[-1]), num_steps)*z_i.units + C_i_i = f_C(np.asarray(z_i_i))*C_i.units + R_i_i = f_R(z_i_i)*R_i.units + + h_i_i = np.ones(z_i_i.size)*np.diff(z_i_i).min() + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i_i, C_i_i, R_i_i, h_i_i, + sigma, plot) + + spline_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i*2, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : num_steps, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + spline_icsd = icsd.SplineiCSD(**spline_input) + csd = spline_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd, decimal=3) + + + def test_SplineiCSD_02(self): + '''test using non-standard SI units''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #construct interpolators, spline method assume underlying source + #pattern generating LFPs that are cubic spline interpolates between + #contacts so we generate CSD data relying on the same assumption + f_C = interp1d(z_i, C_i, kind='cubic') + f_R = interp1d(z_i, R_i) + num_steps = 201 + z_i_i = np.linspace(float(z_i[0]), float(z_i[-1]), num_steps)*z_i.units + C_i_i = f_C(np.asarray(z_i_i))*C_i.units + R_i_i = f_R(z_i_i)*R_i.units + + h_i_i = np.ones(z_i_i.size)*np.diff(z_i_i).min() + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i_i, C_i_i, R_i_i, h_i_i, + sigma, plot) + + spline_input = { + 'lfp' : phi_j*1E3*pq.mV/pq.V, + 'coord_electrode' : z_j, + 'diam' : R_i*2, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : num_steps, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + spline_icsd = icsd.SplineiCSD(**spline_input) + csd = spline_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd, decimal=3) + + + def test_SplineiCSD_03(self): + '''test using standard SI units''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #construct interpolators, spline method assume underlying source + #pattern generating LFPs that are cubic spline interpolates between + #contacts so we generate CSD data relying on the same assumption + f_C = interp1d(z_i, C_i, kind='cubic') + f_R = interp1d(z_i, R_i) + num_steps = 201 + z_i_i = np.linspace(float(z_i[0]), float(z_i[-1]), num_steps)*z_i.units + C_i_i = f_C(np.asarray(z_i_i))*C_i.units + R_i_i = f_R(z_i_i)*R_i.units + + h_i_i = np.ones(z_i_i.size)*np.diff(z_i_i).min() + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i_i, C_i_i, R_i_i, h_i_i, + sigma, plot) + + spline_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j*1E3*pq.mm/pq.m, + 'diam' : R_i*2*1E3*pq.mm/pq.m, + 'sigma' : sigma, + 'sigma_top' : sigma, + 'num_steps' : num_steps, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + spline_icsd = icsd.SplineiCSD(**spline_input) + csd = spline_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd, decimal=3) + + + def test_SplineiCSD_04(self): + '''test using standard SI units''' + #set some parameters for ground truth csd and csd estimates., e.g., + #we will use same source diameter as in ground truth + + #contact point coordinates + z_j = np.arange(21)*1E-4*pq.m + + #source coordinates + z_i = z_j + + #current source density magnitude + C_i = np.zeros(z_i.size)*pq.A/pq.m**3 + C_i[7:12:2] += np.array([-.5, 1., -.5])*pq.A/pq.m**3 + + #source radius (delta, step) + R_i = np.ones(z_i.size)*1E-3*pq.m + + #source height (cylinder) + h_i = np.ones(z_i.size)*1E-4*pq.m + + #conductivity, use same conductivity for top layer (z_j < 0) + sigma = 0.3*pq.S/pq.m + sigma_top = sigma + + #construct interpolators, spline method assume underlying source + #pattern generating LFPs that are cubic spline interpolates between + #contacts so we generate CSD data relying on the same assumption + f_C = interp1d(z_i, C_i, kind='cubic') + f_R = interp1d(z_i, R_i) + num_steps = 201 + z_i_i = np.linspace(float(z_i[0]), float(z_i[-1]), num_steps)*z_i.units + C_i_i = f_C(np.asarray(z_i_i))*C_i.units + R_i_i = f_R(z_i_i)*R_i.units + + h_i_i = np.ones(z_i_i.size)*np.diff(z_i_i).min() + + #flag for debug plots + plot = False + + #get LFP and CSD at contacts + phi_j, C_i = get_lfp_of_cylinders(z_j, z_i_i, C_i_i, R_i_i, h_i_i, + sigma, plot) + + spline_input = { + 'lfp' : phi_j, + 'coord_electrode' : z_j, + 'diam' : R_i*2, + 'sigma' : sigma*1E3*pq.mS/pq.S, + 'sigma_top' : sigma*1E3*pq.mS/pq.S, + 'num_steps' : num_steps, + 'tol' : 1E-12, # Tolerance in numerical integration + 'f_type' : 'gaussian', + 'f_order' : (3, 1), + } + spline_icsd = icsd.SplineiCSD(**spline_input) + csd = spline_icsd.get_csd() + + self.assertEqual(C_i.units, csd.units) + nt.assert_array_almost_equal(C_i, csd, decimal=3) + + +#def suite(verbosity=2): +# ''' +# Run unittests for the CSD toolbox +# +# +# Arguments +# --------- +# verbosity : int +# verbosity level +# +# ''' +# suite = unittest.TestLoader().loadTestsFromTestCase(TestICSD) +# unittest.TextTestRunner(verbosity=verbosity).run(suite) +# +# +# +#if __name__ == '__main__': +# suite() + + +def suite(): + suite = unittest.makeSuite(TestICSD, 'test') + return suite + +if __name__ == "__main__": + runner = unittest.TextTestRunner(verbosity=2) + runner.run(suite()) diff --git a/docs/tutorial/05_opt_extracellular/inputs/virt_spikes.h5 b/docs/tutorial/05_opt_extracellular/inputs/virt_spikes.h5 new file mode 100644 index 00000000..da165786 Binary files /dev/null and b/docs/tutorial/05_opt_extracellular/inputs/virt_spikes.h5 differ diff --git a/docs/tutorial/05_opt_extracellular/plot_ecp.py b/docs/tutorial/05_opt_extracellular/plot_ecp.py new file mode 100644 index 00000000..6f715a63 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/plot_ecp.py @@ -0,0 +1,3 @@ +from bmtk.analyzer.ecp import plot_ecp + +_ = plot_ecp(config_file='config.lfp.json', report_name='cortical_electrode') \ No newline at end of file diff --git a/docs/tutorial/05_opt_extracellular/run_bionet.lfp.py b/docs/tutorial/05_opt_extracellular/run_bionet.lfp.py new file mode 100755 index 00000000..78af3307 --- /dev/null +++ b/docs/tutorial/05_opt_extracellular/run_bionet.lfp.py @@ -0,0 +1,20 @@ +# -*- coding: utf-8 -*- +import os, sys +from bmtk.simulator import bionet + + +def run(config_file): + conf = bionet.Config.from_json(config_file, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + run(sys.argv[-1]) + else: + run('/local1/workspace/Notebooks/bmtk-workshop/opt_external_models/config.hay_syns.json') diff --git a/docs/tutorial/06_opt_hpc_resources/Ch_External_Resources.ipynb b/docs/tutorial/06_opt_hpc_resources/Ch_External_Resources.ipynb new file mode 100644 index 00000000..6091ec54 --- /dev/null +++ b/docs/tutorial/06_opt_hpc_resources/Ch_External_Resources.ipynb @@ -0,0 +1,344 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "6a6332d1-d4a2-4461-857c-64f65fb3b666", + "metadata": {}, + "source": [ + "# External Resources for Large-Scale Network Modeling\n", + "\n", + "Building, simulating, and analyzing large-scale network models can often be a resource intensive process. Although BMTK can often run simple-to-moderate network models using commerically available hardware - many scientists will find themselves quickly requiring access to High-Performance Computing (HPC) resources when the computational, memory, and/or storage requirements become greater than even the best modern laptops and desktops. When this happens you will have to rely on the resources of you institution's computing resources or even on the many Cloud Computing Platforms.\n", + "\n", + "Beyond access to higher computing and memory resources, there are other benefits to using Cloud and HPC resources in your workflow! Using a shared environment can improve collaboration and replication, reducing the headaches and pitfalls when every one has to install the software requirements on their own machines.\n", + "\n", + "In this tutorial we provide a list of tools and resources that neuroscientists can take advantage of.\n", + "\n", + "**Note** - scripts and files for running this tutorial can be found in the directory [06_opt_hpc_resources/](https://github.com/AllenInstitute/bmtk/tree/develop/docs/tutorial/06_opt_hpc_resources)" + ] + }, + { + "cell_type": "markdown", + "id": "7b36a2ec", + "metadata": {}, + "source": [ + "---\n", + "\n", + "## Contents\n", + "1. [Neuroscience Gateway (NSG)](#nsg)\n", + "\n", + " 1.1. [Example: Using NSG to run L4 Model simulations](#example-1)\n", + " \n", + " 1.2. [Example: Using NSG for BioNet (eg. NEURON) Simulations](#example-2)\n", + "\n", + "\n", + "---" + ] + }, + { + "cell_type": "markdown", + "id": "0bdf3e5e-4f4f-4a7e-bd2a-fdfed55612f6", + "metadata": {}, + "source": [ + "## 1. Neuroscience Gateway (NSG) " + ] + }, + { + "cell_type": "markdown", + "id": "1e8b4601-23fc-4283-b661-703d4acb45f5", + "metadata": {}, + "source": [ + "[The Neuroscience Gateway (NSG)](http://www.nsgportal.org/) is an NSF funded project that provides HPC resouces for neuroscienists. It provides access to extensive CPU, GPU, and Memory resources that may not always be available locally. But just as important, NSG includes a wide variety of pre-installed computational neuroscience software that can be used for everything from modeling, data analysis, and even AI and ML applications. For modeling and simulation not only does NSG include BMTK, but also other population tools like NEURON, NEST, Brian, PyNN, NetPyNE, among others. See [https://nsgprod.sdsc.edu:8443/portal2/tools.action](https://nsgprod.sdsc.edu:8443/portal2/tools.action) for a list of available software.\n", + "\n", + "The Neuroscience Gateway can be accessed in two different ways, either through the Web Portal interface or through the REST API. The focus in this tutorial will be on using the Web Portal." + ] + }, + { + "cell_type": "markdown", + "id": "a00fbcc0-f7ab-4b03-9568-e89fa328e9e3", + "metadata": { + "jp-MarkdownHeadingCollapsed": true + }, + "source": [ + "### 1.1. Example: Using NSG to run L4 Model simulations \n", + "\n", + "As an example of how one can utilize NSG running BMTK we will take the [existing Mouse V1 Layer 4 Model](../Ch6_l4model/6.%20More%20Realistic%20Model%20and%20Advanced%20Features.ipynb) network and use it to run simulations of the Expanse computing cluster. " + ] + }, + { + "cell_type": "markdown", + "id": "6abab8e1-7d63-47f9-9790-2b3e07dbcc5e", + "metadata": {}, + "source": [ + "#### Step 1: (Register and) Log-in\n", + "\n", + "If you are planning to use NSG for your research you will first need to register an account at [https://www.nsgportal.org/gest/reg.php](https://www.nsgportal.org/gest/reg.php). It is freely available to most researchers, educators, and students working in the neuroscience field. \n", + "\n", + "After you have registered go ahead and log in to the [NSG Portal](https://nsgprod.sdsc.edu:8443/portal2)\n" + ] + }, + { + "cell_type": "markdown", + "id": "d1a49cda-d9e1-48ed-8fa5-56b3c895ad2d", + "metadata": {}, + "source": [ + "#### Step 2: Packaging the environment files\n", + "\n", + "We will need to package all necessacary files into a .zip folder to be uploaded to NSG. This will include the run_pointnet.py python script to execute, plus any necessary SONATA and model files." + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "3913c8f3-0913-466c-9a8d-ce7143f619ec", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "/bin/sh: 1: zip: not found\r\n" + ] + } + ], + "source": [ + "! cd ../ && zip -rq l4model.zip Ch6_l4model/ " + ] + }, + { + "cell_type": "markdown", + "id": "896ab6e9-921c-418d-9673-476d9b3dfc4b", + "metadata": {}, + "source": [ + "#### Step 3: Setting Up an NSG Folder\n", + "\n", + "1. When you login you will be prompted to \"`Create New Folder`\" that can be used for running BMTK applications. Click on that button, we will give it a \"Label\" of **bmtk_simulations** and an appropiate description:\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "2. Navagate to **bmtk_simulations** > **Data** and select the \"`Upload Data`\" button. Provide an appropiate label (we'll use Label **pointnet_l4model**) and click the \"`Choose File`\" button to upload the previously created zip file. Click \"`Save`\" to upload the file.\n", + "\n", + "
\n", + " \n", + "
\n", + "
" + ] + }, + { + "cell_type": "markdown", + "id": "b0a5f004-efec-418b-8fbd-4decf57188f9", + "metadata": {}, + "source": [ + "#### Step 4: Setup and Run Task\n", + "\n", + "1. Navigate to the **bmtk_simulations** > **Tasks** folder and select \"`Create New Task`\" button.\n", + "\n", + "2. First give a `Description` for the task - we'll call it **pointnet_l4model`\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "\n", + "3. Under the `Select Data` panel choose the uploaded zip file for with the **pointent_l4model** Label and click \"`Select Data`\"\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "\n", + "4. Under the `Select Tool` panel we select the **Python on Expanse** option\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "5. Finally Select the `Set Parameters` Panel to give the run-time parameters. Enter the parameters as specified below and click the \"`Save Parameters`\" button.\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + " - Set **Max Hours to Run: 1.0** - Depending on the size of the network, the **tstop** and **dt**, and the number of Cores we may have to increase/decrease this value.\n", + " - Unselect **Do you require nrnivmodl compilation** - flag only applies to NEURON (eg. BioNet) applications.\n", + " - Set **Enter Main Input Python Filename: run_pointnet.py** - Entry python script to run.\n", + " - Set **Enter sub-directory name: Ch6_l4model** - This is required since the way we zipped up our data everything is under the *Ch6_l4model/* folder.\n", + " - To speed up the simulation we will run it parallelized using MPI with 8 processs. \n", + " - Set **Enter Number of Nodes: 1** - Since we are significantly less than the avaialbe cores/memory per node we can benefit from keeping all MPI Tasks on the same node.\n", + " - Set **Enter Number of MPI Tasks per Node: 8** and **Enter Number of Cores per Node: 8**\n", + " - Set **Enter Number of GB Memory per Node: 16**\n", + "\n", + "---\n", + "**Notes: Building the Network and LGN Inputs**\n", + "\n", + "Running the **run_pointnet.py** script requires that the SONATA network files (*network/*) and lgn inputs (*inputs/spikes.gratings.90deg_4Hz.h5*) already exists. If they don't, or you want to re-generate them, you can replace the **Enter Main Input Python Filename:** option with the *build_network.py* or *run_filternet.py* scripts.\n", + "\n", + "---\n", + "\n", + "6. Now that you have selected the data, the tool, and the parameters click the \"`Save and Run Task`\" button to submit the task to the HPC." + ] + }, + { + "cell_type": "markdown", + "id": "e3bf8ed6-3d5f-41ce-8a4a-629308ca045d", + "metadata": {}, + "source": [ + "#### Fetching the Results\n", + "\n", + "After clickin on `Save and Run Task` the task will be submitted to the NSQ queue. Depending on current HPC usage and the requested resources there may be a delay between the time when a job is submitted and the time the task starts running. You can select the `View Status` button to see the **Status** of the current task.\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n" + ] + }, + { + "cell_type": "markdown", + "id": "5f027d47-1960-488a-a926-8c0a373d4ccf", + "metadata": {}, + "source": [ + "### 1.2. Example: Using NSG for BioNet (eg. NEURON) Simulations
\n", + "\n", + "The above tutorial will work when running *PointNet*, *FilterNet*, *Builder*, or *Analysis* components of BMTK. However running *BioNet* simulations require a slight changes to intialize a task properly since it requires using the NEURON simulation underneath. In this example we will show a how to run a multi-core BioNet simualation using the network generated in [Chapter 3](http://127.0.0.1:8889/lab/tree/bmtk-workshop/Ch3_multicells/3.%20Multipopulation%20biophysical.ipynb) taking note to highlight difference from the example above." + ] + }, + { + "cell_type": "markdown", + "id": "1531052f-e284-462f-9428-5930688fb19f", + "metadata": {}, + "source": [ + "#### Step 1: Packaging Data\n", + "\n", + "We first want to package the *Ch3_mutlicells/* folder into a single zip file so it will contain the python scripts and all necessary network and model files to run the full simulation. **HOWEVER** The modfiles stored in the *components/mechanisms/modfiles/* folder need to be moved to the root directory. NSG will need to compile these files before running the simulation and if they are stored under the *components/* sub-directory they will not be found." + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "dcca7f4e-9945-48b9-b232-e2f683d630cb", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "cp: cannot stat '../Ch3_multicells': No such file or directory\n", + "mv: cannot stat 'Ch3_multicells/components/mechanisms/modfiles/*': No such file or directory\n", + "/bin/sh: 1: zip: not found\n" + ] + } + ], + "source": [ + "! cp -r ../Ch3_multicells .\n", + "! mv Ch3_multicells/components/mechanisms/modfiles/* Ch3_multicells/\n", + "! zip -rq bionet_multicells.zip Ch3_multicells/" + ] + }, + { + "cell_type": "markdown", + "id": "0cdee10f-defa-4ee8-8588-c9ef762860c5", + "metadata": {}, + "source": [ + "#### Step 2: Uploading the Data\n", + "1. Log into NSGPortal and either select `Create New Folder`, or select the existing **bmtk_simulations** folder if it exists.\n", + "\n", + "2. Under **bmtk_simulations** > **Data** folder select `Upload Data` to upload the created *bionet_multicells.zip* folder with an descriptive Label\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + " " + ] + }, + { + "cell_type": "markdown", + "id": "6b7f06a5-4555-4302-89a9-8c6d51df9f82", + "metadata": {}, + "source": [ + "#### Step 3: Creating and Submitting a Task\n", + "\n", + "Under **bmtk_simulations** > **Tasks** select the `Create New Task` button\n", + "\n", + "1. In the `Task Summary` tab give the task an appropiate description (we'll call it **bionet_multicells**)\n", + "\n", + "\n", + "2. In the `Select Data` tab make sure to select the **bionet_multicell** data we uploaded in the previous step and click `Select Data`.\n", + "\n", + "\n", + "3. In the `Select Tool` tab choose option **NEURON on Expanse** \n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "4. In the `Set Parameters` tab we can use the following setup, the click `Save Parameters` button.\n", + "\n", + "
\n", + " \n", + "
\n", + "
\n", + "\n", + "The following parameters are important\n", + "- Make sure the select **Do you required nrnivmodl compilation** in order to compile the .mod file necessacary to run the models\n", + "- Set **Enter Main Input Filename: run_bionet.py** as this is the script to execute the BMTK simulation\n", + "- Make sure to select **Please click here if your code is in python and not HOC**\n", + "- Due to the structure of the zip file we need to set **Enter sub-directory name: Ch3_mutlicells**\n", + "\n", + "Other settings that can be adjusted:\n", + "- For a modest network **Maximum Hours to Run: 0.5** is plenty enough. But for longer simulations or larger networks you may need to increase this value otherwise the job will stop before BMTK has finished.\n", + "- We will set **Number of MPI Tasks per Node** and **Number of Cores per Node** to **8**, and **Number of Nodes** to **1** to parallelize the simulation on 8 cores.\n", + "- For current network **Number of GB Memory per Node: 32** will suffice.\n", + "\n", + "\n", + "5. Finally select `Save and Run Task` to submit the Task to the queue." + ] + }, + { + "cell_type": "markdown", + "id": "73619795-4b8b-4ff6-b613-07ab7e419241", + "metadata": {}, + "source": [ + "---" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "5d65754c-315e-4aad-a2d7-a4d0be89d7f3", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3 (ipykernel)", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.9.7" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/tutorial/06_opt_hpc_resources/_static/_tutorial_images/nsgport_initial.png b/docs/tutorial/06_opt_hpc_resources/_static/_tutorial_images/nsgport_initial.png new file mode 100755 index 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