diff --git a/bmtk/simulator/bionet/__init__.py b/bmtk/simulator/bionet/__init__.py index 3ee0740f..565de735 100644 --- a/bmtk/simulator/bionet/__init__.py +++ b/bmtk/simulator/bionet/__init__.py @@ -20,7 +20,7 @@ # WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # -from bmtk.simulator.bionet.pyfunction_cache import synapse_model, synaptic_weight, cell_model, add_weight_function +from bmtk.simulator.bionet.pyfunction_cache import synapse_model, synaptic_weight, cell_model, add_weight_function, model_processing from bmtk.simulator.bionet.config import Config from bmtk.simulator.bionet.bionetwork import BioNetwork from bmtk.simulator.bionet.biosimulator import BioSimulator diff --git a/bmtk/simulator/core/pyfunction_cache.py b/bmtk/simulator/core/pyfunction_cache.py index 98bb1311..2af2b5c6 100644 --- a/bmtk/simulator/core/pyfunction_cache.py +++ b/bmtk/simulator/core/pyfunction_cache.py @@ -274,6 +274,31 @@ def func_wrapper(*args, **kwargs): return func(*args, **kwargs) return func_wrapper return decorator + + +def model_processing(*wargs, **wkwargs): + if len(wargs) == 1 and callable(wargs[0]): + # for the case without decorator arguments, grab the function object in wargs and create a decorator + func = wargs[0] + py_modules.add_cell_processor(func.__name__, func) # add function assigned to its original name + + @wraps(func) + def func_wrapper(*args, **kwargs): + return func(*args, **kwargs) + return func_wrapper + else: + # for the case with decorator arguments + assert(all(k in ['name'] for k in wkwargs.keys())) + + def decorator(func): + # store the function in py_modules but under the name given in the decorator arguments + py_modules.add_cell_processor(wkwargs['name'], func) + + @wraps(func) + def func_wrapper(*args, **kwargs): + return func(*args, **kwargs) + return func_wrapper + return decorator def add_weight_function(func, name=None, overwrite=True): diff --git a/examples/bio_all_active_sweep/README.md b/examples/bio_all_active_sweep/README.md new file mode 100644 index 00000000..99585e61 --- /dev/null +++ b/examples/bio_all_active_sweep/README.md @@ -0,0 +1,50 @@ +# bio_all_active_sweep + +Contains an example of how to download and "Biophysical, all-active" models from the +(Allen Cell Types Database)[https://celltypes.brain-map.org/data]. Also includes examples of how to +alter the "model_processing" function so that you can run cells with their full axon rather than with +the replacement stubs that is created by default (warning: model parameters were optimized by replacing +morphologicial axon with stubs) + +## Directory Structure +* ephys_inputs/ - Folder containing NWB files downloaded for the Allen Cell Types Database. These files contain different experimental stimulus sweeps (eg square-waves, ramps, noise). Used during simulation to create current clamp stimulus onto the cells. +* network/ - Folder containing SONATA network files, created by the `build_network.py` script and used when running the +* models/ - Folder containing model files (especially parameters json file and morphology swc) downloaded from Allen Cell Types Database. +* output\*/ - Folders containing results from simulations, created by running the run_bionet.py scripts. By default contains simulation spike-times and soma membrane voltage potentials. +* build_network.py - Python script to (re)build the SONATA network/ files, can also be used to download models/ and ephys_inputs/ files. +* run_bionet.py - Python script to execute a simulation. +* config.simulation.\*.json - SONATA config file containg all the information bmtk will require to run a full simulation - including location of network files, download Cell Types DB files, simulation parameters, etc. + + + +## (Re)Building the network + +To download the Cell-Type files and create the SONATA network files required to run a simulation sweep you can run: +```bash + $ python build_network.py +``` + +If you want to try running a simulation with a different cell model just replace the `specimen_id` value in the python file. The script will also attempt to automatically download the model files and ephys_data nwb files if not already in the folder (if you've manually download these files the script will not try to download it again). + +You can also change `axon_type` to either **full** or **stub**. The default behavior is a stub axon (eg. It removes the axon and replaces it with a simple small column). But you can change it full in which case when the simulation runs it will simulate the cell using full morphology reconstruction. + + + +## Running the simulation + +To run a simulation with a given `config.simulation.\*.json` file run the following python command: + +```bash + $ python run_bionet.py config.simulation.json +``` + +BMTK will automatically load in the configuration file, run a full simulation, and save the results into the output\*/ folder (as specified in the configuration json). + +If you want to run a simulation with a different cell model, sweep number, or axon type you can do the following: +1. Copy one of the existing `config.simulation.\*.json` files and open it up with your preferred text editor. +2. In the "manifest" section update the following params as needed. + 1. **SWEEP_NUM** - Change to run with a different stimulus sweep. + 2. **AXON_TYPE** - Set to either *fullaxon* or *stubaxon* depending on how to handle the model's axon. + 3. **MODEL_ID** - Set the the model being used. +3. For some stimulus sweeps you may also need to adjust **run/tstop** (the run time in ms) otherwise the simulation may stop before sweep has finished. + diff --git a/examples/bio_all_active_sweep/build_network.py b/examples/bio_all_active_sweep/build_network.py new file mode 100644 index 00000000..aca15a15 --- /dev/null +++ b/examples/bio_all_active_sweep/build_network.py @@ -0,0 +1,33 @@ +from bmtk.builder.networks import NetworkBuilder +from download_data import download_ephys_data, download_model, compile_mechanisms + + +def build_network(specimen_id=488683425, network_dir='network', axon_type='full', fetch_data=True): + processing_func = 'aibs_allactive' if axon_type == 'stub' else 'aibs_allactive_fullaxon' + + # Get data from Allen CellTypes database. + if fetch_data: + model_dir = download_model(specimen_id=specimen_id) + download_ephys_data(specimen_id=specimen_id) + else: + model_dir='models/neuronal_model_48868368425' + + # Builds a SONATA network consisting of a single cell + net = NetworkBuilder(f'bio_{axon_type}axon') + net.add_nodes( + N=1, + model_type='biophysical', + model_template='ctdb:Biophys1.hoc', + model_processing=processing_func, + morphology=f'reconstruction.swc', + dynamics_params=f'fit_parameters.json', + + specimen_id=specimen_id + ) + net.build() + net.save(output_dir=network_dir) + + +if __name__ == '__main__': + build_network(axon_type='full') + build_network(axon_type='stub') diff --git a/examples/bio_all_active_sweep/config.simulation.491766131_fullaxon.sweep35.json b/examples/bio_all_active_sweep/config.simulation.491766131_fullaxon.sweep35.json new file mode 100644 index 00000000..3d595185 --- /dev/null +++ b/examples/bio_all_active_sweep/config.simulation.491766131_fullaxon.sweep35.json @@ -0,0 +1,70 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$SWEEP_NUM": "35", + "$AXON_TYPE": "fullaxon", + "$MODEL_ID": "491766131", + "$OUTPUT_DIR": "$BASE_DIR/output_${MODEL_ID}_${AXON_TYPE}_sweep${SWEEP_NUM}", + "$INPUT_DIR": "$BASE_DIR/ephys_inputs", + "$NETWORK_DIR": "$BASE_DIR/network", + "$MODEL_DIR": "$BASE_DIR/models/neuronal_model_${MODEL_ID}" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_nwb": { + "module": "IClamp", + "input_type": "allen", + "node_set": "all", + "file": "${INPUT_DIR}/488683423_ephys.nwb", + "sweep_id": "${SWEEP_NUM}" + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": [0], + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "$MODEL_DIR", + "mechanisms_dir":"$MODEL_DIR", + "biophysical_neuron_models_dir": "$MODEL_DIR" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/bio_${AXON_TYPE}_nodes.h5", + "node_types_file": "$NETWORK_DIR/bio_${AXON_TYPE}_node_types.csv" + } + ] + } + } \ No newline at end of file diff --git a/examples/bio_all_active_sweep/config.simulation.491766131_stubaxon.sweep35.json b/examples/bio_all_active_sweep/config.simulation.491766131_stubaxon.sweep35.json new file mode 100644 index 00000000..92082c47 --- /dev/null +++ b/examples/bio_all_active_sweep/config.simulation.491766131_stubaxon.sweep35.json @@ -0,0 +1,70 @@ +{ + "manifest": { + "$BASE_DIR": ".", + "$SWEEP_NUM": "35", + "$AXON_TYPE": "stubaxon", + "$MODEL_ID": "491766131", + "$OUTPUT_DIR": "$BASE_DIR/output_${MODEL_ID}_${AXON_TYPE}_sweep${SWEEP_NUM}", + "$INPUT_DIR": "$BASE_DIR/ephys_inputs", + "$NETWORK_DIR": "$BASE_DIR/network", + "$MODEL_DIR": "$BASE_DIR/models/neuronal_model_${MODEL_ID}" + }, + + "run": { + "tstop": 3000.0, + "dt": 0.1, + "dL": 20.0, + "spike_threshold": -15, + "nsteps_block": 5000 + }, + + "target_simulator":"NEURON", + + "conditions": { + "celsius": 34.0, + "v_init": -80 + }, + + "inputs": { + "current_clamp_nwb": { + "module": "IClamp", + "input_type": "allen", + "node_set": "all", + "file": "${INPUT_DIR}/488683423_ephys.nwb", + "sweep_id": 35 + } + }, + + "output":{ + "log_file": "log.txt", + "output_dir": "$OUTPUT_DIR", + "spikes_file": "spikes.h5", + "spikes_file_csv": "spikes.csv", + "spikes_sort_order": "time", + "overwrite_output_dir": true + }, + + "reports": { + "membrane_potential": { + "cells": [0], + "variable_name": "v", + "module": "membrane_report", + "sections": "soma" + } + }, + + "components": { + "morphologies_dir": "$MODEL_DIR", + "mechanisms_dir":"$MODEL_DIR", + "biophysical_neuron_models_dir": "$MODEL_DIR" + }, + + "networks": { + "nodes": [ + { + "nodes_file": "$NETWORK_DIR/bio_${AXON_TYPE}_nodes.h5", + "node_types_file": "$NETWORK_DIR/bio_${AXON_TYPE}_node_types.csv" + } + ] + } + } \ No newline at end of file diff --git a/examples/bio_all_active_sweep/download_data.py b/examples/bio_all_active_sweep/download_data.py new file mode 100644 index 00000000..6813c563 --- /dev/null +++ b/examples/bio_all_active_sweep/download_data.py @@ -0,0 +1,96 @@ +from pathlib import Path +import os +import io + +import xml.etree.ElementTree as ET +import requests +import zipfile + + +def download_model(specimen_id, model_type='Biophysical - all active', base_dir='models', overwrite=False): + """Uses the Allen REST API to find and download the model files for a given cell. All relevant files (parameters json and morphology swc) + will be saved in a separate folder, the path to which will be returned. + + Notes: Using the REST API because at the moment I don't think the AllenSDK is capable of downloading parameters_fit.json files, see + https://community.brain-map.org/t/cell-types-database-api/3016 for more info on how to use the API. + """ + # Set a request to get fetch model data available for a given speciment_id. It will return a xml string that needs to be parsed so + # that we can find the correct model_id. + api_url = f"http://api.brain-map.org/api/v2/data/query.xml?criteria=model::NeuronalModel,rma::critera,[specimen_id$eq{specimen_id}]" + response = requests.get(api_url) + xml_root = ET.fromstring(response.content) + model_id = None + for (model_name, model_id) in zip(xml_root.iter('name'), xml_root.iter('id')): + if 'Biophysical - all active' in model_name.text: + model_id = int(model_id.text) + break + + if model_id is None: + raise ValueError(f'Could not find a "{model_type}" model for cell {specimen_id}') + + # Now that we have the model_id for the given cell we can download and unzip the files into the correct directory. To prevent downloading + # the zip everytime we'll check to see if the directory already exists. + model_dir = Path(f'{base_dir}/neuronal_model_{model_id}') + if model_dir.exists() and not overwrite: + print(f'> {model_dir} already exits, skipping donwloadng data') + return model_dir + + zip_uri = f'http://api.brain-map.org/neuronal_model/download/{model_id}' + zip_response = requests.get(zip_uri) + zip_file = zipfile.ZipFile(io.BytesIO(zip_response.content)) + zip_file.extractall(model_dir) + return model_dir + + +def download_ephys_data(specimen_id, base_dir='ephys_inputs'): + """Download nwb file containing sweeps.""" + api_url = f'http://api.brain-map.org/api/v2/data/query.xml?criteria=model::Specimen,rma::criteria,[id$eq{specimen_id}],rma::include,ephys_result(well_known_files(well_known_file_type[name$eqNWBDownload]))' + response = requests.get(api_url) + # print(response.content) + download_uri = None + xml_root = ET.fromstring(response.content) + for dl_link in xml_root.iter('download-link'): + download_uri = dl_link.text + break + + ephys_id = None + for erid in xml_root.iter('ephys-result-id'): + ephys_id = erid.text + break + + nwb_path = Path(f'{base_dir}/{ephys_id}_ephys.nwb') + if nwb_path.exists(): + print(f'> {nwb_path} already exits, skipping donwload.') + return + + if not Path(base_dir).exists(): + os.makedirs(base_dir) + + url_req = f'https://celltypes.brain-map.org/{download_uri}' + nwb_req = requests.get(url_req, stream=True) + with open(nwb_path, 'wb') as fh: + for chunk in nwb_req.iter_content(): + fh.write(chunk) + + +def compile_mechanisms(model_dir, modfiles_dir='modfiles', overwrite=False): + from subprocess import call + import platform + + print(model_dir) + if not Path(f'{model_dir}/{modfiles_dir}').is_dir(): + print(f'> Could not find directory {model_dir}/{modfiles_dir}, skipping compiling.') + + if Path(platform.machine()).is_dir(): + print(f'> {Path(platform.machine())} already existing, skipping compiling') + + cwd = os.getcwd() + os.chdir(model_dir) + call(['nrnivmodl', 'modfiles']) + os.chdir(cwd) + + +if __name__ == '__main__': + model_dir = download_model(specimen_id=488683425) + download_ephys_data(specimen_id=488683425) + # compile_mechanisms(model_dir) diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/README b/examples/bio_all_active_sweep/models/neuronal_model_491766131/README new file mode 100644 index 00000000..557730be --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/README @@ -0,0 +1 @@ +Please visit http://alleninstitute.github.io/AllenSDK/ for instructions on running this model. diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/ephys_sweeps.json b/examples/bio_all_active_sweep/models/neuronal_model_491766131/ephys_sweeps.json new file mode 100644 index 00000000..97e0e8fe --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/ephys_sweeps.json @@ -0,0 +1,1130 @@ +[ + { + "bridge_balance_mohm": 14.1025638580322, + "id": 488683610, + "leak_pa": -73.1305465698242, + "num_spikes": null, + "peak_deflection": null, + "post_noise_rms_mv": null, + "post_vm_mv": null, + "pre_noise_rms_mv": null, + "pre_vm_mv": null, + "slow_noise_rms_mv": null, + "slow_vm_mv": null, + "specimen_id": 488683425, + "stimulus_absolute_amplitude": null, + "stimulus_description": "EXTPGGAEND141203[0]", + "stimulus_duration": null, + "stimulus_interval": null, + "stimulus_name": "Test", + "stimulus_relative_amplitude": 1.0, + "stimulus_start_time": null, + "stimulus_units": "Volts", + "sweep_number": 71, + "vm_delta_mv": null + }, + { + "bridge_balance_mohm": 12.3931617736816, + "id": 488683504, + "leak_pa": 13.0544996261597, + "num_spikes": 1, + "peak_deflection": null, + "post_noise_rms_mv": 0.0333027169108391, + "post_vm_mv": -67.9999313354492, + "pre_noise_rms_mv": 0.0345665886998177, + "pre_vm_mv": -68.0134506225586, + "slow_noise_rms_mv": 0.142555892467499, + "slow_vm_mv": -68.0134506225586, + "specimen_id": 488683425, + "stimulus_absolute_amplitude": 690.000001579705, + "stimulus_description": "C1SSFINEST150112[0]", + "stimulus_duration": 0.002995, + "stimulus_interval": null, + "stimulus_name": "Short Square", + "stimulus_relative_amplitude": 6.90000009536743, + "stimulus_start_time": 1.02, + "stimulus_units": "Amps", + "sweep_number": 18, + "vm_delta_mv": 0.013519287109375 + }, + { + "bridge_balance_mohm": 14.1025638580322, + "id": 488683600, + "leak_pa": -73.1305465698242, + "num_spikes": 43, + "peak_deflection": null, + "post_noise_rms_mv": 0.0450246930122375, + "post_vm_mv": -68.7638702392578, + "pre_noise_rms_mv": 0.037558414041996, + "pre_vm_mv": -69.4275207519531, + "slow_noise_rms_mv": 0.231182977557182, + "slow_vm_mv": -69.4275207519531, + "specimen_id": 488683425, + "stimulus_absolute_amplitude": 249.999992929517, + "stimulus_description": "C2SQRHELNG150216[0]", + "stimulus_duration": 1.999995, + "stimulus_interval": null, + "stimulus_name": "Square - 2s Suprathreshold", + "stimulus_relative_amplitude": 2.5, + "stimulus_start_time": 1.02, + "stimulus_units": "Amps", + "sweep_number": 66, + "vm_delta_mv": 0.663650512695312 + }, + { + "bridge_balance_mohm": 14.1025638580322, + "id": 488683564, + "leak_pa": -33.7769317626953, + "num_spikes": 165, + "peak_deflection": null, + "post_noise_rms_mv": 0.0322532691061497, + "post_vm_mv": -68.2352447509766, + "pre_noise_rms_mv": 0.0386939980089664, + "pre_vm_mv": -68.9396209716797, + "slow_noise_rms_mv": 0.225896999239922, + "slow_vm_mv": -68.9396209716797, + "specimen_id": 488683425, + "stimulus_absolute_amplitude": 400.750016504858, + "stimulus_description": "C1NSSEED_2150216[0]", + "stimulus_duration": 18.999995, + "stimulus_interval": 8.0, + "stimulus_name": "Noise 2", + "stimulus_relative_amplitude": 1.70000004768372, + "stimulus_start_time": 2.02, + "stimulus_units": "Amps", + "sweep_number": 48, + "vm_delta_mv": 0.704376220703125 + }, + { + "bridge_balance_mohm": 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"slow_noise_rms_mv": 0.218209832906723, + "slow_vm_mv": -69.6565170288086, + "specimen_id": 488683425, + "stimulus_absolute_amplitude": 129.99999687846, + "stimulus_description": "C1LSCOARSE150216[12]", + "stimulus_duration": 0.999995, + "stimulus_interval": null, + "stimulus_name": "Long Square", + "stimulus_relative_amplitude": 1.0, + "stimulus_start_time": 1.02, + "stimulus_units": "Amps", + "sweep_number": 34, + "vm_delta_mv": 0.460174560546875 + }, + { + "bridge_balance_mohm": 12.3931617736816, + "id": 488683496, + "leak_pa": 13.0544996261597, + "num_spikes": null, + "peak_deflection": null, + "post_noise_rms_mv": 0.0362660810351372, + "post_vm_mv": -68.5066604614258, + "pre_noise_rms_mv": 0.0341960713267326, + "pre_vm_mv": -68.358757019043, + "slow_noise_rms_mv": 0.105923421680927, + "slow_vm_mv": -68.358757019043, + "specimen_id": 488683425, + "stimulus_absolute_amplitude": 629.999996615282, + "stimulus_description": "C1SSFINEST150112[0]", + "stimulus_duration": 0.002995, + 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"488683423_ephys.nwb", + "key": "stimulus_path" + }, + { + "parent_key": "WORKDIR", + "type": "file", + "format": "NWB", + "spec": "488683423_ephys.nwb", + "key": "output_path" + } + ] +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/model_metadata.json b/examples/bio_all_active_sweep/models/neuronal_model_491766131/model_metadata.json new file mode 100644 index 00000000..99bd1361 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/model_metadata.json @@ -0,0 +1,143 @@ +{ + "id": 491766131, + "name": "Biophysical - all active_Rbp4-Cre_KL100;Ai14-203503.04.01.01", + "neuron_reconstruction_id": 527109145, + "neuronal_model_template_id": 491455321, + "specimen_id": 488683425, + "specimen": { + "cell_prep_sample_id": null, + "cell_reporter_id": 491913822, + "cortex_layer_id": null, + "data": null, + "donor_id": 485250259, + "ephys_result_id": 488683423, + "external_specimen_name": null, + "failed_facet": 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485250259, + "initial_kps": null, + "mgmt_ihc": null, + "mgmt_methylation": null, + "molecular_subtype": null, + "multifocal": null, + "name": "Rbp4-Cre_KL100;Ai14-203503", + "organism_id": 2, + "pmi": null, + "primary_tissue_source": null, + "pten_deletion": null, + "race_only": null, + "radiation_therapy": null, + "recurrence_by_six_months": null, + "sex": "M", + "sex_full_name": "Male", + "sleep_state": null, + "smoker": null, + "strain": null, + "strain_only": "", + "survival_days": null, + "tags": "Rbp4-Cre_KL100;Ai14-203503 ", + "theiler_stage": null, + "time_to_progression_or_recurrence": null, + "transgenic_mouse_id": 34, + "tumor_status": null, + "weight_grams": null, + "transgenic_lines": [ + { + "description": "tdTomato fluorescent protein is expressed in cytoplasm", + "id": 177836291, + "name": "Ai14(RCL-tdT)", + "originating_lab": "Allen Institute for Brain Science", + "stock_number": "007914", + "sub_image_annotation_id": 21592, + "transgenic_line_source_name": "JAX", + "transgenic_line_type_code": "R", + "transgenic_line_type_name": "reporter", + "url_prefix": "http://jaxmice.jax.org/strain/", + "url_suffix": ".html" + }, + { + "description": "Enriched in cortical layer 5 and the dentate gyrus.", + "id": 177838435, + "name": "Rbp4-Cre_KL100", + "originating_lab": "Nathaniel Heintz and Charles Gerfen", + "stock_number": "031125", + "sub_image_annotation_id": 120818676, + "transgenic_line_source_name": "MMRRC", + "transgenic_line_type_code": "D", + "transgenic_line_type_name": "driver", + "url_prefix": "http://www.mmrrc.org/catalog/getSDS.jsp?mmrrc_id=", + "url_suffix": null + } + ] + }, + "specimen_tags": [ + { + "name": "apical - intact" + }, + { + "name": "dendrite type - spiny" + } + ], + "structure": { + "acronym": "VISp5", + "atlas_id": 1087, + "color_hex_triplet": "08858C", + "depth": 8, + "failed": false, + "failed_facet": 734881840, + "graph_id": 1, + "graph_order": 189, + "hemisphere_id": 3, + "id": 778, + "name": "Primary visual area, layer 5", + "neuro_name_structure_id": null, + "neuro_name_structure_id_path": null, + "ontology_id": 1, + "parent_structure_id": 385, + "safe_name": "Primary visual area layer 5", + "sphinx_id": 190, + "st_level": 11, + "structure_id_path": "/997/8/567/688/695/315/669/385/778/", + "structure_name_facet": 1453946728, + "weight": 8690 + } + }, + "neuronal_model_runs": [ + { + "explained_variance_ratio": 0.783997426309877, + "rheobase_feature_average": 3.75733173243137 + } + ] +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/CaDynamics.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/CaDynamics.mod new file mode 100644 index 00000000..12af0659 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/CaDynamics.mod @@ -0,0 +1,40 @@ +: Dynamics that track inside calcium concentration +: modified from Destexhe et al. 1994 + +NEURON { + SUFFIX CaDynamics + USEION ca READ ica WRITE cai + RANGE decay, gamma, minCai, depth +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + FARADAY = (faraday) (coulombs) + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) +} + +PARAMETER { + gamma = 0.05 : percent of free calcium (not buffered) + decay = 80 (ms) : rate of removal of calcium + depth = 0.1 (um) : depth of shell + minCai = 1e-4 (mM) +} + +ASSIGNED {ica (mA/cm2)} + +INITIAL { + cai = minCai +} + +STATE { + cai (mM) +} + +BREAKPOINT { SOLVE states METHOD cnexp } + +DERIVATIVE states { + cai' = -(10000)*(ica*gamma/(2*FARADAY*depth)) - (cai - minCai)/decay +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ca_HVA.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ca_HVA.mod new file mode 100644 index 00000000..84db2d3c --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ca_HVA.mod @@ -0,0 +1,82 @@ +: Reference: Reuveni, Friedman, Amitai, and Gutnick, J.Neurosci. 1993 + +NEURON { + SUFFIX Ca_HVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + UNITSOFF + : if((v == -27) ){ + : v = v+0.0001 + : } + :mAlpha = (0.055*(-27-v))/(exp((-27-v)/3.8) - 1) + mAlpha = 0.055 * vtrap(-27 - v, 3.8) + mBeta = (0.94*exp((-75-v)/17)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + hAlpha = (0.000457*exp((-13-v)/50)) + hBeta = (0.0065/(exp((-v-15)/28)+1)) + hInf = hAlpha/(hAlpha + hBeta) + hTau = 1/(hAlpha + hBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ca_LVA.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ca_LVA.mod new file mode 100644 index 00000000..ab151d0e --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ca_LVA.mod @@ -0,0 +1,69 @@ +: Comment: LVA ca channel. Note: mtau is an approximation from the plots +: Reference: Avery and Johnston 1996, tau from Randall 1997 +: Comment: shifted by -10 mv to correct for junction potential +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Ca_LVA + USEION ca READ eca WRITE ica + RANGE gbar, g, ica +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + eca (mV) + ica (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ica = g*(v-eca) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + v = v + 10 + mInf = 1.0000/(1+ exp((v - -30.000)/-6)) + mTau = (5.0000 + 20.0000/(1+exp((v - -25.000)/5)))/qt + hInf = 1.0000/(1+ exp((v - -80.000)/6.4)) + hTau = (20.0000 + 50.0000/(1+exp((v - -40.000)/7)))/qt + v = v - 10 + UNITSON +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ih.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ih.mod new file mode 100644 index 00000000..73b97d84 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Ih.mod @@ -0,0 +1,71 @@ +: Reference: Kole,Hallermann,and Stuart, J. Neurosci. 2006 + +NEURON { + SUFFIX Ih + NONSPECIFIC_CURRENT ihcn + RANGE gbar, g, ihcn +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + ehcn = -45.0 (mV) +} + +ASSIGNED { + v (mV) + ihcn (mA/cm2) + g (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ihcn = g*(v-ehcn) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + : if(v == -154.9){ + : v = v + 0.0001 + : } + :mAlpha = 0.001*6.43*(v+154.9)/(exp((v+154.9)/11.9)-1) + mAlpha = 0.001 * 6.43 * vtrap(v + 154.9, 11.9) + mBeta = 0.001*193*exp(v/33.1) + mInf = mAlpha/(mAlpha + mBeta) + mTau = 1/(mAlpha + mBeta) + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Im.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Im.mod new file mode 100644 index 00000000..d6112d57 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Im.mod @@ -0,0 +1,62 @@ +: Reference: Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones +: Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Im + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Im_v2.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Im_v2.mod new file mode 100644 index 00000000..fc219f71 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Im_v2.mod @@ -0,0 +1,59 @@ +: Based on Im model of Vervaeke et al. (2006) + +NEURON { + SUFFIX Im_v2 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-30)/10) + mAlpha = 0.007 * exp( (6 * 0.4 * (v - (-48))) / 26.12 ) + mBeta = 0.007 * exp( (-6 * (1 - 0.4) * (v - (-48))) / 26.12 ) + + mInf = mAlpha / (mAlpha + mBeta) + mTau = (15 + 1 / (mAlpha + mBeta)) / qt +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/K_P.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/K_P.mod new file mode 100644 index 00000000..0a1238f9 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/K_P.mod @@ -0,0 +1,71 @@ +: Comment: The persistent component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + + +NEURON { + SUFFIX K_P + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + tauF = 1 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mInf = 1 / (1 + exp(-(v - (-14.3 + vshift)) / 14.6)) + if (v < -50 + vshift){ + mTau = tauF * (1.25+175.03*exp(-(v - vshift) * -0.026))/qt + } else { + mTau = tauF * (1.25+13*exp(-(v - vshift) * 0.026))/qt + } + hInf = 1/(1 + exp(-(v - (-54 + vshift))/-11)) + hTau = (360+(1010+24*(v - (-55 + vshift)))*exp(-((v - (-75 + vshift))/48)^2))/qt + UNITSON +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/K_T.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/K_T.mod new file mode 100644 index 00000000..c31beaff --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/K_T.mod @@ -0,0 +1,68 @@ +: Comment: The transient component of the K current +: Reference: Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats:subtypes and gradients,Korngreen and Sakmann, J. Physiology, 2000 + +NEURON { + SUFFIX K_T + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) + mTauF = 1.0 + hTauF = 1.0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*m*h + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1/(1 + exp(-(v - (-47 + vshift)) / 29)) + mTau = (0.34 + mTauF * 0.92*exp(-((v+71-vshift)/59)^2))/qt + hInf = 1/(1 + exp(-(v+66-vshift)/-10)) + hTau = (8 + hTauF * 49*exp(-((v+73-vshift)/23)^2))/qt + UNITSON +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kd.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kd.mod new file mode 100644 index 00000000..82cbe59a --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kd.mod @@ -0,0 +1,62 @@ +: Based on Kd model of Foust et al. (2011) + + +NEURON { + SUFFIX Kd + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + hInf + hTau +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * h + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h' = (hInf - h) / hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-23)/10) + mInf = 1 - 1 / (1 + exp((v - (-43)) / 8)) + mTau = 1 + hInf = 1 / (1 + exp((v - (-67)) / 7.3)) + hTau = 1500 +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kv2like.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kv2like.mod new file mode 100644 index 00000000..6b45acd6 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kv2like.mod @@ -0,0 +1,89 @@ +: Kv2-like channel +: Adapted from model implemented in Keren et al. 2005 +: Adjusted parameters to be similar to guangxitoxin-sensitive current in mouse CA1 pyramids from Liu and Bean 2014 + + +NEURON { + SUFFIX Kv2like + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mAlpha + mBeta + mTau + hInf + h1Tau + h2Tau +} + +STATE { + m + h1 + h2 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * m * m * (0.5 * h1 + 0.5 * h2) + ik = g * (v - ek) +} + +DERIVATIVE states { + rates() + m' = (mInf - m) / mTau + h1' = (hInf - h1) / h1Tau + h2' = (hInf - h2) / h2Tau +} + +INITIAL{ + rates() + m = mInf + h1 = hInf + h2 = hInf +} + +PROCEDURE rates() { + LOCAL qt + qt = 2.3^((celsius-21)/10) + UNITSOFF + mAlpha = 0.12 * vtrap( -(v - 43), 11.0) + mBeta = 0.02 * exp(-(v + 1.27) / 120) + mInf = mAlpha / (mAlpha + mBeta) + mTau = 2.5 * (1 / (qt * (mAlpha + mBeta))) + + hInf = 1/(1 + exp((v + 58) / 11)) + h1Tau = (360 + (1010 + 23.7 * (v + 54)) * exp(-((v + 75) / 48)^2)) / qt + h2Tau = (2350 + 1380 * exp(-0.011 * v) - 210 * exp(-0.03 * v)) / qt + if (h2Tau < 0) { + h2Tau = 1e-3 + } + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kv3_1.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kv3_1.mod new file mode 100644 index 00000000..e2446577 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Kv3_1.mod @@ -0,0 +1,54 @@ +: Comment: Kv3-like potassium current + +NEURON { + SUFFIX Kv3_1 + USEION k READ ek WRITE ik + RANGE gbar, g, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + vshift = 0 (mV) +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + g (S/cm2) + mInf + mTau +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m + ik = g*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + UNITSOFF + mInf = 1/(1+exp(((v -(18.700 + vshift))/(-9.700)))) + mTau = 0.2*20.000/(1+exp(((v -(-46.560 + vshift))/(-44.140)))) + UNITSON +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaTa.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaTa.mod new file mode 100644 index 00000000..fcf7bd39 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaTa.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTa + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -48 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -69 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) : ng - adjusted this to match actual Colbert & Pan values for soma model + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaTs.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaTs.mod new file mode 100644 index 00000000..f753e718 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaTs.mod @@ -0,0 +1,95 @@ +: Reference: Colbert and Pan 2002 + +NEURON { + SUFFIX NaTs + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + + malphaF = 0.182 + mbetaF = 0.124 + mvhalf = -40 (mV) + mk = 6 (mV) + + halphaF = 0.015 + hbetaF = 0.015 + hvhalf = -66 (mV) + hk = 6 (mV) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + mTau + mAlpha + mBeta + hInf + hTau + hAlpha + hBeta +} + +STATE { + m + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar*m*m*m*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + m = mInf + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-23)/10) + + UNITSOFF + mAlpha = malphaF * vtrap(-(v - mvhalf), mk) + mBeta = mbetaF * vtrap((v - mvhalf), mk) + + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + + hAlpha = halphaF * vtrap(v - hvhalf, hk) + hBeta = hbetaF * vtrap(-(v - hvhalf), hk) + + hInf = hAlpha/(hAlpha + hBeta) + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaV.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaV.mod new file mode 100644 index 00000000..a7023956 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/NaV.mod @@ -0,0 +1,186 @@ +TITLE Mouse sodium current +: Kinetics of Carter et al. (2012) +: Based on 37 degC recordings from mouse hippocampal CA1 pyramids + +NEURON { + SUFFIX NaV + USEION na READ ena WRITE ina + RANGE g, gbar +} + +UNITS { + (mV) = (millivolt) + (S) = (siemens) +} + +PARAMETER { + gbar = .015 (S/cm2) + + : kinetic parameters + Con = 0.01 (/ms) : closed -> inactivated transitions + Coff = 40 (/ms) : inactivated -> closed transitions + Oon = 8 (/ms) : open -> Ineg transition + Ooff = 0.05 (/ms) : Ineg -> open transition + alpha = 400 (/ms) + beta = 12 (/ms) + gamma = 250 (/ms) : opening + delta = 60 (/ms) : closing + + alfac = 2.51 + btfac = 5.32 + + : Vdep + x1 = 24 (mV) : Vdep of activation (alpha) + x2 = -24 (mV) : Vdep of deactivation (beta) +} + +ASSIGNED { + + : rates + f01 (/ms) + f02 (/ms) + f03 (/ms) + f04 (/ms) + f0O (/ms) + f11 (/ms) + f12 (/ms) + f13 (/ms) + f14 (/ms) + f1n (/ms) + fi1 (/ms) + fi2 (/ms) + fi3 (/ms) + fi4 (/ms) + fi5 (/ms) + fin (/ms) + + b01 (/ms) + b02 (/ms) + b03 (/ms) + b04 (/ms) + b0O (/ms) + b11 (/ms) + b12 (/ms) + b13 (/ms) + b14 (/ms) + b1n (/ms) + bi1 (/ms) + bi2 (/ms) + bi3 (/ms) + bi4 (/ms) + bi5 (/ms) + bin (/ms) + + v (mV) + ena (mV) + ina (milliamp/cm2) + g (S/cm2) + celsius (degC) +} + +STATE { + C1 FROM 0 TO 1 + C2 FROM 0 TO 1 + C3 FROM 0 TO 1 + C4 FROM 0 TO 1 + C5 FROM 0 TO 1 + I1 FROM 0 TO 1 + I2 FROM 0 TO 1 + I3 FROM 0 TO 1 + I4 FROM 0 TO 1 + I5 FROM 0 TO 1 + O FROM 0 TO 1 + I6 FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE activation METHOD sparse + g = gbar * O + ina = g * (v - ena) +} + +INITIAL { + rates(v) + SOLVE seqinitial +} + +KINETIC activation +{ + rates(v) + ~ C1 <-> C2 (f01,b01) + ~ C2 <-> C3 (f02,b02) + ~ C3 <-> C4 (f03,b03) + ~ C4 <-> C5 (f04,b04) + ~ C5 <-> O (f0O,b0O) + ~ O <-> I6 (fin,bin) + ~ I1 <-> I2 (f11,b11) + ~ I2 <-> I3 (f12,b12) + ~ I3 <-> I4 (f13,b13) + ~ I4 <-> I5 (f14,b14) + ~ I5 <-> I6 (f1n,b1n) + ~ C1 <-> I1 (fi1,bi1) + ~ C2 <-> I2 (fi2,bi2) + ~ C3 <-> I3 (fi3,bi3) + ~ C4 <-> I4 (fi4,bi4) + ~ C5 <-> I5 (fi5,bi5) + + CONSERVE C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +LINEAR seqinitial { : sets initial equilibrium + ~ I1*bi1 + C2*b01 - C1*( fi1+f01) = 0 + ~ C1*f01 + I2*bi2 + C3*b02 - C2*(b01+fi2+f02) = 0 + ~ C2*f02 + I3*bi3 + C4*b03 - C3*(b02+fi3+f03) = 0 + ~ C3*f03 + I4*bi4 + C5*b04 - C4*(b03+fi4+f04) = 0 + ~ C4*f04 + I5*bi5 + O*b0O - C5*(b04+fi5+f0O) = 0 + ~ C5*f0O + I6*bin - O*(b0O+fin) = 0 + + ~ C1*fi1 + I2*b11 - I1*( bi1+f11) = 0 + ~ I1*f11 + C2*fi2 + I3*b12 - I2*(b11+bi2+f12) = 0 + ~ I2*f12 + C3*fi3 + I4*bi3 - I3*(b12+bi3+f13) = 0 + ~ I3*f13 + C4*fi4 + I5*b14 - I4*(b13+bi4+f14) = 0 + ~ I4*f14 + C5*fi5 + I6*b1n - I5*(b14+bi5+f1n) = 0 + + ~ C1 + C2 + C3 + C4 + C5 + O + I1 + I2 + I3 + I4 + I5 + I6 = 1 +} + +PROCEDURE rates(v(mV) ) +{ + LOCAL qt + qt = 2.3^((celsius-37)/10) + + f01 = qt * 4 * alpha * exp(v/x1) + f02 = qt * 3 * alpha * exp(v/x1) + f03 = qt * 2 * alpha * exp(v/x1) + f04 = qt * 1 * alpha * exp(v/x1) + f0O = qt * gamma + f11 = qt * 4 * alpha * alfac * exp(v/x1) + f12 = qt * 3 * alpha * alfac * exp(v/x1) + f13 = qt * 2 * alpha * alfac * exp(v/x1) + f14 = qt * 1 * alpha * alfac * exp(v/x1) + f1n = qt * gamma + fi1 = qt * Con + fi2 = qt * Con * alfac + fi3 = qt * Con * alfac^2 + fi4 = qt * Con * alfac^3 + fi5 = qt * Con * alfac^4 + fin = qt * Oon + + b01 = qt * 1 * beta * exp(v/x2) + b02 = qt * 2 * beta * exp(v/x2) + b03 = qt * 3 * beta * exp(v/x2) + b04 = qt * 4 * beta * exp(v/x2) + b0O = qt * delta + b11 = qt * 1 * beta * exp(v/x2) / btfac + b12 = qt * 2 * beta * exp(v/x2) / btfac + b13 = qt * 3 * beta * exp(v/x2) / btfac + b14 = qt * 4 * beta * exp(v/x2) / btfac + b1n = qt * delta + bi1 = qt * Coff + bi2 = qt * Coff / (btfac) + bi3 = qt * Coff / (btfac^2) + bi4 = qt * Coff / (btfac^3) + bi5 = qt * Coff / (btfac^4) + bin = qt * Ooff +} + diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Nap.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Nap.mod new file mode 100644 index 00000000..ef8021ec --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/Nap.mod @@ -0,0 +1,77 @@ +:Reference : Modeled according to kinetics derived from Magistretti & Alonso 1999 +:Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +NEURON { + SUFFIX Nap + USEION na READ ena WRITE ina + RANGE gbar, g, ina +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + g (S/cm2) + celsius (degC) + mInf + hInf + hTau + hAlpha + hBeta +} + +STATE { + h +} + +BREAKPOINT { + SOLVE states METHOD cnexp + rates() + g = gbar*mInf*h + ina = g*(v-ena) +} + +DERIVATIVE states { + rates() + h' = (hInf-h)/hTau +} + +INITIAL{ + rates() + h = hInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((celsius-21)/10) + + UNITSOFF + mInf = 1.0/(1+exp((v- -52.6)/-4.6)) : assuming instantaneous activation as modeled by Magistretti and Alonso + + hInf = 1.0/(1+exp((v- -48.8)/10)) + hAlpha = 2.88e-6 * vtrap(v + 17, 4.63) + hBeta = 6.94e-6 * vtrap(-(v + 64.4), 2.63) + + hTau = (1/(hAlpha + hBeta))/qt + UNITSON +} + +FUNCTION vtrap(x, y) { : Traps for 0 in denominator of rate equations + UNITSOFF + if (fabs(x / y) < 1e-6) { + vtrap = y * (1 - x / y / 2) + } else { + vtrap = x / (exp(x / y) - 1) + } + UNITSON +} \ No newline at end of file diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/SK.mod b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/SK.mod new file mode 100644 index 00000000..8bfa3b72 --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/modfiles/SK.mod @@ -0,0 +1,56 @@ +: SK-type calcium-activated potassium current +: Reference : Kohler et al. 1996 + +NEURON { + SUFFIX SK + USEION k READ ek WRITE ik + USEION ca READ cai + RANGE gbar, g, ik +} + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (milli/liter) +} + +PARAMETER { + v (mV) + gbar = .000001 (mho/cm2) + zTau = 1 (ms) + ek (mV) + cai (mM) +} + +ASSIGNED { + zInf + ik (mA/cm2) + g (S/cm2) +} + +STATE { + z FROM 0 TO 1 +} + +BREAKPOINT { + SOLVE states METHOD cnexp + g = gbar * z + ik = g * (v - ek) +} + +DERIVATIVE states { + rates(cai) + z' = (zInf - z) / zTau +} + +PROCEDURE rates(ca(mM)) { + if(ca < 1e-7){ + ca = ca + 1e-07 + } + zInf = 1/(1 + (0.00043 / ca)^4.8) +} + +INITIAL { + rates(cai) + z = zInf +} diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/reconstruction.marker b/examples/bio_all_active_sweep/models/neuronal_model_491766131/reconstruction.marker new file mode 100644 index 00000000..15bee36d --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/reconstruction.marker @@ -0,0 +1,18 @@ +##x,y,z,radius,shape,name,comment, color_r,color_g,color_b +398.7229,550.8966,14.3469,0.0, 1, 10, , 19,247,59 +456.591,582.344,16.035,0.0, 1, 10, , 19,247,59 +419.9418,611.9279,14.3469,0.0, 1, 10, , 19,247,59 +400.0877,621.5489,14.3469,0.0, 1, 10, , 19,247,59 +339.6822,619.0973,14.9097,0.0, 1, 10, , 19,247,59 +384.8565,645.9836,14.9097,0.0, 1, 10, , 19,247,59 +350.3031,679.7774,15.4722,0.0, 1, 10, , 19,247,59 +469.6131,687.8597,16.035,0.0, 1, 10, , 19,247,59 +293.5744,672.4741,16.035,0.0, 1, 10, , 19,247,59 +396.4772,708.7949,14.9097,0.0, 1, 10, , 19,247,59 +322.5176,694.3279,15.4722,0.0, 1, 10, , 19,247,59 +324.491,713.4773,14.9097,0.0, 1, 10, , 19,247,59 +249.4526,725.6506,15.4722,0.0, 1, 10, , 19,247,59 +420.3285,787.6703,14.3469,0.0, 1, 10, , 19,247,59 +454.4197,766.4789,16.5978,0.0, 1, 10, , 19,247,59 +357.6121,705.6455,13.16,0.0, 1, 30, , 19,247,59 +330.7418,762.6922,22.7872,0.0, 1, 20, , 19,247,59 diff --git a/examples/bio_all_active_sweep/models/neuronal_model_491766131/reconstruction.swc b/examples/bio_all_active_sweep/models/neuronal_model_491766131/reconstruction.swc new file mode 100644 index 00000000..f7c402fe --- /dev/null +++ b/examples/bio_all_active_sweep/models/neuronal_model_491766131/reconstruction.swc @@ -0,0 +1,4855 @@ +# generated by Vaa3D Plugin sort_neuron_swc +# source file(s): C:/Users/alexh/Desktop/Working/Rbp4-Cre_KL100;Ai14-203503.04.01.01_515570710_p_Dendrite.swc +# id,type,x,y,z,r,pid +1 1 357.4977 705.5311 27.0085 6.9553 -1 +2 3 351.3693 708.5444 25.6116 0.4347 1 +3 3 350.3283 709.0054 25.3873 0.4347 2 +4 3 349.2403 709.3349 25.1681 0.4347 3 +5 3 348.1341 709.6083 24.9556 0.4347 4 +6 3 347.0221 709.5328 24.7604 0.4347 5 +7 3 345.909 709.2754 24.5857 0.4347 6 +8 3 344.7959 709.018 24.4289 0.4347 7 +9 3 343.6713 708.8235 24.288 0.4347 8 +10 3 342.5948 709.0752 24.1598 0.4347 9 +11 3 341.5423 709.518 24.043 0.4347 10 +12 3 340.4899 709.9618 23.9364 0.4347 11 +13 3 339.5163 710.5476 23.8728 0.4347 12 +14 3 338.5828 711.2054 23.8571 0.4347 13 +15 3 337.6516 711.8666 23.8748 0.4347 14 +16 3 336.6106 712.3093 23.8879 0.4347 15 +17 3 335.494 712.5404 23.87 0.4347 16 +18 3 334.3672 712.7257 23.819 0.4347 17 +19 3 333.2472 712.9534 23.742 0.4347 18 +20 3 332.1764 713.3423 23.66 0.4347 19 +21 3 331.1674 713.8777 23.5827 0.4347 20 +22 3 330.4147 714.7129 23.5127 0.4347 21 +23 3 329.6745 715.5834 23.445 0.4347 22 +24 3 328.6323 715.5571 23.3433 0.4347 23 +25 3 327.4997 715.4553 23.212 0.4347 24 +26 3 326.5068 715.9655 23.088 0.4347 25 +27 3 325.4074 716.247 22.9754 0.4347 26 +28 3 324.2668 716.3122 22.867 0.4347 27 +29 3 323.5198 717.1393 22.7256 0.4347 28 +30 3 322.6503 717.8692 22.5649 0.4347 29 +31 3 321.5532 718.1769 22.4045 0.4347 30 +32 3 320.4516 718.4732 22.2466 0.4347 31 +33 3 319.4345 718.9937 22.1113 0.4347 32 +34 3 318.5514 719.7213 22.0153 0.4347 33 +35 3 317.2621 719.91 21.9128 0.3362 34 +36 3 316.1295 720.0771 21.882 0.2502 35 +37 3 315.0301 720.3848 21.8529 0.2163 36 +38 3 313.9857 720.8481 21.819 0.2301 37 +39 3 312.9561 721.3446 21.7762 0.3421 38 +40 3 312.0569 722.0356 21.6896 0.3575 39 +41 3 311.1142 722.6717 21.5709 0.2901 40 +42 3 309.9988 722.8238 21.4634 0.2884 41 +43 3 308.8766 723.0366 21.3716 0.2589 42 +44 3 307.8412 723.5136 21.2934 0.2167 43 +45 3 306.7419 723.8122 21.2128 0.1931 44 +46 3 305.6093 723.9552 21.1221 0.2272 45 +47 3 304.4745 724.0834 21.0207 0.2552 46 +48 3 303.3613 724.3362 20.9188 0.2498 47 +49 3 302.2997 724.7583 20.8342 0.2886 48 +50 3 301.2575 725.2285 20.755 0.3534 49 +51 3 300.2245 725.7158 20.6774 0.393 50 +52 3 299.1915 726.2043 20.6041 0.4004 51 +53 3 298.115 726.5876 20.5495 0.3903 52 +54 3 296.9801 726.6127 20.5514 0.3735 53 +55 3 295.8407 726.6345 20.5946 0.3331 54 +56 3 294.8077 727.1081 20.6315 0.3029 55 +57 3 293.7838 727.6172 20.6553 0.3739 56 +58 3 292.6661 727.8437 20.65 0.4602 57 +59 3 291.5255 727.9078 20.6108 0.4971 58 +60 3 290.385 727.9638 20.545 0.4775 59 +61 3 289.2432 728.0187 20.4672 0.4102 60 +62 3 288.1233 728.2452 20.4036 0.3205 61 +63 3 287.0285 728.5758 20.3655 0.3283 62 +64 3 285.9348 728.9122 20.3524 0.4131 63 +65 3 284.8011 729.0655 20.3599 0.4576 64 +66 3 283.6662 729.2039 20.3815 0.4002 65 +67 3 282.5669 728.9248 20.4495 0.4006 66 +68 3 281.4618 728.6548 20.5484 0.3405 67 +69 3 280.463 729.2131 20.6184 0.2965 68 +70 3 279.4643 729.7725 20.6595 0.3438 69 +71 3 278.4668 730.3307 20.6713 0.3789 70 +72 3 277.468 730.889 20.6548 0.4704 71 +73 3 276.4579 731.4244 20.5906 0.5113 72 +74 3 275.4489 731.9587 20.4932 0.5261 73 +75 3 274.4387 732.4929 20.3762 0.5061 74 +76 3 273.4286 733.0272 20.246 0.4713 75 +77 3 271.8979 733.0477 19.9578 0.1716 76 +78 3 270.7791 732.8693 19.8022 0.1716 77 +79 3 269.7014 732.494 19.6636 0.1716 78 +80 3 268.5815 732.3854 19.5378 0.1716 79 +81 3 267.4386 732.4243 19.4177 0.1716 80 +82 3 266.2969 732.3751 19.2959 0.1716 81 +83 3 265.8839 731.6017 19.0691 0.1716 82 +84 3 265.7912 730.4818 18.797 0.1716 83 +85 3 265.2684 729.4899 18.5422 0.1716 84 +86 3 264.3349 729.2439 18.3067 0.1716 85 +87 3 264.0798 730.3388 18.0603 0.1716 86 +88 3 263.3522 731.1281 17.7324 0.1716 87 +89 3 262.2597 731.2803 17.3813 0.1716 88 +90 3 261.1409 731.0812 17.0551 0.1716 89 +91 3 260.0278 730.8295 16.7664 0.1716 90 +92 3 258.997 730.8215 16.525 0.1716 91 +93 3 258.1573 731.4644 16.3548 0.1716 92 +94 3 257.1689 731.1567 16.24 0.1716 93 +95 3 256.6976 730.1271 16.133 0.1716 94 +96 3 256.272 729.1067 16.0152 0.1716 95 +97 3 255.3717 728.609 15.9485 0.1716 96 +98 3 254.373 729.046 15.8774 0.1716 97 +99 3 253.7014 729.9143 15.724 0.1716 98 +100 3 252.8618 730.1637 15.5246 0.1716 99 +101 3 251.8447 729.6558 15.3518 0.1716 100 +102 3 250.8037 729.2039 15.1816 0.1716 101 +103 3 249.9834 728.4397 15.0405 0.1716 102 +104 3 249.1106 727.7053 14.9579 0.1716 103 +105 3 249.0202 726.6276 14.9654 0.1716 104 +106 3 249.3382 725.5362 15.1922 0.1716 105 +107 3 272.1176 734.1551 20.12 0.1144 76 +108 3 271.2504 734.8987 20.0903 0.1748 107 +109 3 270.3844 735.6435 20.0665 0.22 108 +110 3 269.2598 735.7716 20.0802 0.2263 109 +111 3 268.1639 736.0931 20.1522 0.2718 110 +112 3 267.1949 736.6914 20.295 0.3061 111 +113 3 266.2271 737.2943 20.4966 0.3835 112 +114 3 265.5418 738.1832 20.7838 0.3469 113 +115 3 264.8955 739.0984 21.1299 0.3517 114 +116 3 264.5511 740.1634 21.5026 0.2359 115 +117 3 264.2446 741.2411 21.8756 0.1694 116 +118 3 264.4516 742.3645 22.1519 0.1271 117 +119 3 264.6941 743.4833 22.3378 0.1144 118 +120 3 264.9355 744.601 22.5072 0.1144 119 +121 3 317.3033 718.8187 21.324 0.1144 34 +122 3 316.5242 718.0305 21.0554 0.2505 121 +123 3 315.6971 717.693 20.6049 0.2232 122 +124 3 314.8265 717.5465 20.1555 0.2127 123 +125 3 314.2751 716.5455 19.7809 0.2263 124 +126 3 313.3141 716.6702 19.423 0.3204 125 +127 3 312.2342 716.4403 19.0828 0.3891 126 +128 3 311.2961 715.8031 18.7816 0.3437 127 +129 3 311.2321 714.6671 18.5545 0.2969 128 +130 3 310.3672 715.2196 18.3579 0.2569 129 +131 3 309.5058 714.4703 18.1692 0.2994 130 +132 3 308.6501 714.6122 17.9458 0.385 131 +133 3 308.3057 713.5048 17.33 0.3961 132 +134 3 307.2212 713.4545 16.9532 0.3319 133 +135 3 306.0806 713.4053 16.6474 0.3949 134 +136 3 305.0064 713.0335 16.4164 0.3149 135 +137 3 303.9917 712.5061 16.2481 0.321 136 +138 3 302.9301 712.823 16.1249 0.2698 137 +139 3 301.8078 713.0232 16.0292 0.2493 138 +140 3 301.3651 713.5837 15.9006 0.1932 139 +141 3 301.7643 714.6248 15.6934 0.1762 140 +142 3 301.8604 715.7253 15.4669 0.1808 141 +143 3 301.7723 716.8601 15.2566 0.2429 142 +144 3 301.134 717.6792 15.118 0.2119 143 +145 3 300.1948 718.3268 15.0679 0.178 144 +146 3 299.2555 718.9743 15.1922 0.2288 145 +147 3 350.1509 704.7177 26.6764 0.4004 1 +148 3 349.0115 704.6205 26.6613 0.4004 147 +149 3 347.8687 704.6068 26.6689 0.4004 148 +150 3 346.735 704.5232 26.7347 0.4004 149 +151 3 345.6093 704.6502 26.8467 0.4004 150 +152 3 344.582 705.1364 26.9564 0.4004 151 +153 3 343.462 705.3103 27.0567 0.4004 152 +154 3 342.4038 704.8893 27.1471 0.4004 153 +155 3 341.2895 705.0941 27.2602 0.4004 154 +156 3 340.1741 705.3263 27.3888 0.4004 155 +157 3 339.0336 705.2634 27.5153 0.4004 156 +158 3 337.9365 704.9397 27.6284 0.4004 157 +159 3 336.8268 704.6628 27.7393 0.4004 158 +160 3 335.6954 704.5198 27.8681 0.4004 159 +161 3 334.5628 704.3791 28.0076 0.4004 160 +162 3 333.4291 704.2487 28.1537 0.4004 161 +163 3 332.2931 704.132 28.3021 0.4004 162 +164 3 331.1571 704.0142 28.4491 0.4004 163 +165 3 330.0211 703.8975 28.588 0.4004 164 +166 3 328.8817 703.7991 28.7062 0.4004 165 +167 3 327.7434 703.6996 28.8044 0.4004 166 +168 3 326.604 703.6012 28.8828 0.4004 167 +169 3 325.4657 703.5028 28.9425 0.4004 168 +170 3 324.3263 703.401 28.9828 0.4004 169 +171 3 323.2017 703.2168 28.9582 0.4004 170 +172 3 322.076 703.0338 28.8862 0.4004 171 +173 3 320.9503 702.8358 28.8033 0.4004 172 +174 3 319.8246 702.6299 28.728 0.4004 173 +175 3 318.7378 702.273 28.6608 0.4004 174 +176 3 317.6899 701.8165 28.6009 0.4004 175 +177 3 316.6409 701.3589 28.5477 0.4004 176 +178 3 315.7566 701.4894 28.6966 0.1144 177 +179 3 314.6252 701.6564 28.6978 0.1373 178 +180 3 313.4938 701.8234 28.6983 0.1373 179 +181 3 312.431 702.2066 28.6989 0.1374 180 +182 3 311.4334 702.7661 28.7 0.1374 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374.9906 652.5639 30.3106 0.3598 1598 +1600 4 375.2137 651.4416 30.3551 0.4005 1599 +1601 4 375.8612 650.5024 30.3808 0.4354 1600 +1602 4 376.4549 649.5243 30.3906 0.4682 1601 +1603 4 376.6837 648.4043 30.3932 0.4543 1602 +1604 4 376.9423 647.2901 30.3965 0.4492 1603 +1605 4 377.2718 646.1941 30.401 0.4525 1604 +1606 4 377.6218 645.105 30.4074 0.5119 1605 +1607 4 378.0119 644.0297 30.4167 0.4773 1606 +1608 4 378.2693 642.9154 30.4296 0.4305 1607 +1609 4 378.4466 641.7851 30.4478 0.4869 1608 +1610 4 378.7132 640.672 30.4724 0.469 1609 +1611 4 378.9992 639.5646 30.5032 0.4581 1610 +1612 4 379.7176 639.202 29.5534 0.1144 1611 +1613 4 380.7358 638.8782 28.7255 0.3636 1612 +1614 4 381.8214 638.6311 28.3968 0.3216 1613 +1615 4 382.5845 637.9081 27.9944 0.2481 1614 +1616 4 382.4289 636.8877 27.592 0.2047 1615 +1617 4 381.9107 635.881 27.1933 0.2468 1616 +1618 4 382.3168 635.0207 26.8103 0.301 1617 +1619 4 381.961 634.2096 26.439 0.2878 1618 +1620 4 380.8296 634.2485 26.0848 0.244 1619 +1621 4 379.7245 634.3263 25.6995 0.2568 1620 +1622 4 378.5931 634.2668 25.3607 0.282 1621 +1623 4 377.6081 633.7668 25.0463 0.3216 1622 +1624 4 376.8416 632.934 24.7386 0.3146 1623 +1625 4 376.09 632.1069 24.4577 0.2346 1624 +1626 4 375.4345 631.44 24.164 0.1834 1625 +1627 4 374.3328 631.7111 23.8602 0.1883 1626 +1628 4 373.4416 631.297 23.5488 0.17 1627 +1629 4 372.4441 631.5921 23.1963 0.1557 1628 +1630 4 371.3916 631.9605 22.813 0.1477 1629 +1631 4 370.4684 631.3278 22.4325 0.1571 1630 +1632 4 369.8666 630.3703 22.1458 0.1713 1631 +1633 4 368.7878 630.7021 21.9052 0.2135 1632 +1634 4 367.7674 631.21 21.7076 0.2142 1633 +1635 4 366.7447 631.7202 21.5678 0.2488 1634 +1636 4 365.7219 632.2327 21.4852 0.266 1635 +1637 4 364.6992 632.7464 21.4393 0.2977 1636 +1638 4 363.6787 633.2623 21.4141 0.2428 1637 +1639 4 362.6709 633.8057 21.4085 0.2347 1638 +1640 4 361.6664 634.3526 21.4152 0.2217 1639 +1641 4 360.6471 634.8502 21.427 0.1894 1640 +1642 4 359.5123 634.8994 21.4404 0.1686 1641 +1643 4 358.4335 634.5562 21.4715 0.1691 1642 +1644 4 357.4977 633.9144 21.5298 0.2095 1643 +1645 4 356.594 633.3653 21.5614 0.2069 1644 +1646 4 355.546 633.8035 21.5494 0.2349 1645 +1647 4 354.5645 634.0803 21.4659 0.2428 1646 +1648 4 353.4891 633.7382 21.3038 0.2414 1647 +1649 4 352.678 633.1491 21.0921 0.2023 1648 +1650 4 351.9688 632.3426 20.8516 0.1928 1649 +1651 4 350.8991 631.9891 20.6161 0.2123 1650 +1652 4 349.8066 631.6882 20.3675 0.2972 1651 +1653 4 348.7907 631.186 20.1228 0.3332 1652 +1654 4 347.8332 630.5991 19.8523 0.3021 1653 +1655 4 347.617 631.5097 19.5639 0.262 1654 +1656 4 346.6023 631.6882 19.3239 0.2175 1655 +1657 4 345.5029 631.3873 19.1349 0.2185 1656 +1658 4 344.6655 630.614 18.9871 0.2726 1657 +1659 4 343.9562 629.724 18.8451 0.3474 1658 +1660 4 342.9895 629.1256 18.6805 0.382 1659 +1661 4 342.0045 628.5479 18.517 0.3797 1660 +1662 4 341.1946 627.7586 18.3565 0.3538 1661 +1663 4 340.8491 626.6729 18.1686 0.297 1662 +1664 4 340.531 625.6399 17.9382 0.2344 1663 +1665 4 339.5243 625.1388 17.691 0.2534 1664 +1666 4 338.4158 624.9397 17.409 0.205 1665 +1667 4 337.5578 624.4844 17.1324 0.1782 1666 +1668 4 337.6802 623.377 16.9078 0.1665 1667 +1669 4 338.4124 622.7089 16.7177 0.1892 1668 +1670 4 339.4099 623.1128 16.5222 0.2445 1669 +1671 4 340.4247 623.2912 16.345 0.2827 1670 +1672 4 341.3044 622.6712 16.1804 0.3228 1671 +1673 4 341.4234 621.6073 15.9799 0.317 1672 +1674 4 340.7564 620.7664 15.8147 0.2386 1673 +1675 4 339.6376 620.6623 15.7181 0.1921 1674 +1676 4 338.5451 620.3294 15.6206 0.1992 1675 +1677 4 338.4947 619.3124 15.3566 0.2161 1676 +1678 4 339.5678 618.9829 14.6297 0.1144 1677 +1679 4 371.2349 630.8565 21.9428 0.1144 1631 +1680 4 372.2633 630.3875 21.5429 0.2655 1679 +1681 4 373.135 629.6919 21.4203 0.3344 1680 +1682 4 374.0022 629.0032 21.2836 0.3567 1681 +1683 4 374.6726 629.1554 21.037 0.3381 1682 +1684 4 374.6646 630.2776 20.7547 0.2512 1683 +1685 4 375.4894 630.6094 20.4842 0.2295 1684 +1686 4 376.0179 629.5947 20.2636 0.1994 1685 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1773 +1775 4 333.4657 603.9268 33.4281 0.1769 1774 +1776 4 332.3469 604.1578 33.5423 0.2361 1775 +1777 4 331.2155 604.3306 33.6109 0.1967 1776 +1778 4 330.084 604.4999 33.6395 0.1627 1777 +1779 4 328.9538 604.6704 33.6378 0.1373 1778 +1780 4 327.8143 604.763 33.6014 0.1373 1779 +1781 4 326.6761 604.7321 33.5216 0.1373 1780 +1782 4 325.5378 604.8099 33.4281 0.1373 1781 +1783 4 324.4613 605.1932 33.3598 0.1373 1782 +1784 4 323.3859 605.5833 33.3178 0.1373 1783 +1785 4 322.3117 605.9768 33.3029 0.1373 1784 +1786 4 321.2009 606.2525 33.3158 0.1373 1785 +1787 4 320.0901 606.5259 33.3547 0.1373 1786 +1788 4 318.9792 606.7982 33.4121 0.1373 1787 +1789 4 317.8615 607.0396 33.49 0.1373 1788 +1790 4 316.7324 607.083 33.6356 0.1373 1789 +1791 4 315.6021 607.1208 33.8288 0.1373 1790 +1792 4 314.4639 607.1974 34.0158 0.1373 1791 +1793 4 313.3256 607.3118 34.1603 0.1373 1792 +1794 4 312.1919 607.4594 34.265 0.1373 1793 +1795 4 311.0593 607.6219 34.335 0.1373 1794 +1796 4 309.9268 607.7832 34.3767 0.1373 1795 +1797 4 308.7942 607.9445 34.4047 0.1373 1796 +1798 4 307.6822 608.2133 34.4378 0.1373 1797 +1799 4 306.6595 608.7235 34.4901 0.1373 1798 +1800 4 305.6379 609.2361 34.5562 0.1373 1799 +1801 4 304.6163 609.7486 34.6304 0.1373 1800 +1802 4 303.5947 610.2611 34.7074 0.1373 1801 +1803 4 302.5514 610.729 34.7777 0.1373 1802 +1804 4 301.4646 611.0848 34.827 0.1373 1803 +1805 4 300.3766 611.4394 34.8583 0.1373 1804 +1806 4 299.2887 611.794 34.8757 0.1373 1805 +1807 4 298.179 612.072 34.8838 0.1373 1806 +1808 4 297.035 612.056 34.886 0.1373 1807 +1809 4 295.891 612.04 34.886 0.1373 1808 +1810 4 294.747 612.0228 34.886 0.1373 1809 +1811 4 293.603 612.0068 34.886 0.1373 1810 +1812 4 292.4602 611.9908 34.886 0.1373 1811 +1813 4 291.3162 611.9748 34.886 0.1373 1812 +1814 4 290.1722 611.9588 34.886 0.1372 1813 +1815 4 289.0282 611.9428 34.886 0.1372 1814 +1816 4 287.8842 611.9256 34.886 0.1371 1815 +1817 4 286.7402 611.9096 34.886 0.1368 1816 +1818 4 285.5962 611.8936 34.886 0.1364 1817 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0.1374 1883 +1885 4 370.6846 570.1044 27.2317 0.1374 1884 +1886 4 370.0977 569.1228 27.111 0.1376 1885 +1887 4 369.512 568.1413 26.9903 0.1379 1886 +1888 4 368.932 567.1563 26.8699 0.1383 1887 +1889 4 368.352 566.1713 26.7484 0.1392 1888 +1890 4 367.772 565.1863 26.6244 0.1409 1889 +1891 4 367.192 564.2014 26.4972 0.144 1890 +1892 4 366.5937 563.229 26.3626 0.1497 1891 +1893 4 365.8958 562.3286 26.2052 0.1608 1892 +1894 4 365.0893 561.5576 26.0285 0.1788 1893 +1895 4 364.0059 561.1903 25.8661 0.2248 1894 +1896 4 363.0381 560.6961 25.7082 0.2484 1895 +1897 4 362.3803 559.7695 25.5321 0.249 1896 +1898 4 361.6276 558.9138 25.3613 0.2294 1897 +1899 4 360.8039 558.121 25.2118 0.1803 1898 +1900 4 359.9813 557.3282 25.0796 0.1511 1899 +1901 4 359.1577 556.5354 24.9609 0.1393 1900 +1902 4 358.3134 555.7666 24.8592 0.1411 1901 +1903 4 357.4085 555.0665 24.7811 0.1444 1902 +1904 4 356.4601 554.4304 24.7201 0.1503 1903 +1905 4 355.4385 553.9191 24.6674 0.1619 1904 +1906 4 354.3689 553.5141 24.6156 0.1809 1905 +1907 4 353.2718 553.2041 24.5473 0.2281 1906 +1908 4 352.1507 552.9936 24.4507 0.2574 1907 +1909 4 351.2057 552.544 24.3407 0.2515 1908 +1910 4 350.6017 551.5807 24.2432 0.3033 1909 +1911 4 349.6979 550.9561 24.1604 0.3241 1910 +1912 4 348.6065 550.6152 24.0887 0.2698 1911 +1913 4 347.5827 550.1324 24.0153 0.2767 1912 +1914 4 346.6354 549.4929 23.9268 0.225 1913 +1915 4 345.6882 548.8546 23.8221 0.2138 1914 +1916 4 344.7398 548.2162 23.7006 0.2401 1915 +1917 4 343.7651 547.6214 23.5749 0.2887 1916 +1918 4 342.7699 547.0597 23.4441 0.2647 1917 +1919 4 341.7894 546.4774 23.294 0.2034 1918 +1920 4 340.7884 545.9317 23.1269 0.1705 1919 +1921 4 339.7028 545.7097 22.9594 0.1755 1920 +1922 4 338.7407 545.3173 22.7772 0.2063 1921 +1923 4 337.8461 544.6355 22.5624 0.2744 1922 +1924 4 336.7936 544.2283 22.335 0.347 1923 +1925 4 335.6725 544.0406 22.1119 0.399 1924 +1926 4 334.5662 544.2077 21.9131 0.3243 1925 +1927 4 333.81 544.9478 21.7126 0.3302 1926 +1928 4 333.3925 546.0037 21.5054 0.3268 1927 +1929 4 333.0516 547.094 21.3212 0.2749 1928 +1930 4 332.6683 548.1705 21.1436 0.286 1929 +1931 4 332.0037 549.0617 20.932 0.2436 1930 +1932 4 330.9455 549.2699 20.7264 0.2417 1931 +1933 4 329.8404 549.5204 20.5201 0.3231 1932 +1934 4 328.8348 550.0238 20.2756 0.2911 1933 +1935 4 327.7274 550.1622 20.008 0.3184 1934 +1936 4 326.9724 550.9332 19.7386 0.2922 1935 +1937 4 326.1452 551.7134 19.4995 0.273 1936 +1938 4 325.0848 552.1264 19.3242 0.3239 1937 +1939 4 324.0334 552.576 19.206 0.341 1938 +1940 4 322.9604 552.973 19.1349 0.4049 1939 +1941 4 321.8507 553.2498 19.0949 0.3672 1940 +1942 4 320.9709 553.9694 19.0742 0.3689 1941 +1943 4 320.1415 554.7565 19.0498 0.368 1942 +1944 4 319.136 555.2884 18.9916 0.2715 1943 +1945 4 318.1098 555.7941 18.9375 0.2095 1944 +1946 4 317.063 556.2414 18.9431 0.2132 1945 +1947 4 316.0048 556.6681 18.9907 0.2161 1946 +1948 4 314.8757 556.8512 19.0347 0.2415 1947 +1949 4 313.7466 557.0342 19.075 0.3051 1948 +1950 4 312.6174 557.2172 19.131 0.4576 1949 +1951 4 383.0764 597.0158 29.0072 0.1144 1858 +1952 4 383.5889 596.1441 28.3777 0.1381 1951 +1953 4 384.0522 595.3147 28.1249 0.1388 1952 +1954 4 383.7033 594.2737 27.7782 0.14 1953 +1955 4 383.9767 593.5118 27.3722 0.1423 1954 +1956 4 385.0098 593.0427 26.9564 0.1467 1955 +1957 4 386.005 592.4959 26.5457 0.1546 1956 +1958 4 386.6411 591.6196 26.0966 0.1697 1957 +1959 4 386.7635 590.5511 25.5861 0.1956 1958 +1960 4 386.9443 589.5318 24.9866 0.2534 1959 +1961 4 387.4751 588.548 24.4625 0.3145 1960 +1962 4 387.1376 587.5401 23.9952 0.3072 1961 +1963 4 386.6354 586.5231 23.5631 0.3075 1962 +1964 4 387.6204 586.117 23.1333 0.2406 1963 +1965 4 388.3605 585.2567 22.7461 0.2152 1964 +1966 4 389.2151 584.5108 22.3826 0.2613 1965 +1967 4 390.1715 583.9068 22.0192 0.238 1966 +1968 4 390.5227 582.8302 21.6642 0.265 1967 +1969 4 391.224 581.9425 21.3209 0.2028 1968 +1970 4 392.1083 581.2538 20.9975 0.1748 1969 +1971 4 392.3577 580.1418 20.7281 0.1571 1970 +1972 4 392.2513 579.0081 20.466 0.1868 1971 +1973 4 392.1895 577.887 20.2065 0.1671 1972 +1974 4 392.8016 576.9787 19.9178 0.1503 1973 +1975 4 393.8266 576.616 19.6658 0.1379 1974 +1976 4 394.0851 575.535 19.3998 0.1383 1975 +1977 4 393.9799 574.3978 19.1618 0.1391 1976 +1978 4 393.8117 573.2721 18.9417 0.1407 1977 +1979 4 393.6813 572.1487 18.7216 0.1436 1978 +1980 4 393.9467 571.142 18.548 0.1494 1979 +1981 4 393.9215 570.4236 18.4019 0.1571 1980 +1982 4 393.7362 569.4992 18.2339 0.1868 1981 +1983 4 394.4638 568.6732 18.0482 0.1671 1982 +1984 4 395.3904 568.0029 17.8783 0.1503 1983 +1985 4 396.4143 567.519 17.6893 0.1379 1984 +1986 4 397.4782 567.1151 17.4726 0.1383 1985 +1987 4 398.4277 566.5019 17.2483 0.1392 1986 +1988 4 398.7092 565.4517 16.9929 0.1409 1987 +1989 4 399.6084 564.874 16.758 0.1439 1988 +1990 4 400.0213 563.8856 16.4858 0.1502 1989 +1991 4 400.0076 562.7519 16.2201 0.1586 1990 +1992 4 399.6598 561.6823 15.9298 0.1894 1991 +1993 4 399.1816 560.6481 15.6545 0.1721 1992 +1994 4 398.6943 559.6173 15.3992 0.1594 1993 +1995 4 398.231 558.5786 15.1455 0.1551 1994 +1996 4 398.0125 557.4701 14.8932 0.1683 1995 +1997 4 398.39 556.397 14.6983 0.2048 1996 +1998 4 398.3511 555.261 14.5228 0.2132 1997 +1999 4 398.104 554.1513 14.35 0.1738 1998 +2000 4 398.08 553.0085 14.2257 0.1398 1999 +2001 4 398.3305 551.8919 14.1459 0.1144 2000 +2002 4 398.6085 550.7822 14.0669 0.1144 2001 +2003 4 388.8467 605.5764 31.5028 0.1992 1848 +2004 4 389.5778 604.6967 31.4191 0.2544 2003 +2005 4 389.9358 603.6202 31.3113 0.2285 2004 +2006 4 390.1818 602.5151 31.1864 0.2319 2005 +2007 4 390.4278 601.41 31.0607 0.2164 2006 +2008 4 391.1794 601.0919 30.3848 0.1144 2007 +2009 4 392.2536 600.7121 30.1896 0.1373 2008 +2010 4 393.3221 600.8025 30.1185 0.1373 2009 +2011 4 394.3482 601.0187 30.0023 0.1373 2010 +2012 4 395.4385 600.7281 29.822 0.1373 2011 +2013 4 396.5401 600.4581 29.6187 0.1373 2012 +2014 4 397.6601 600.2271 29.4406 0.1373 2013 +2015 4 398.7915 600.0703 29.2992 0.1373 2014 +2016 4 399.9332 600.0269 29.1894 0.1373 2015 +2017 4 401.0624 599.9067 29.0984 0.1373 2016 +2018 4 402.1675 599.6127 29.0074 0.1374 2017 +2019 4 403.2646 599.2958 28.9047 0.1374 2018 +2020 4 404.3628 598.979 28.7854 0.1375 2019 +2021 4 405.4553 598.6449 28.6496 0.1377 2020 +2022 4 406.5261 598.2514 28.4959 0.1382 2021 +2023 4 407.5786 597.8098 28.3262 0.139 2022 +2024 4 408.6299 597.3671 28.1476 0.1405 2023 +2025 4 409.6813 596.9255 27.9644 0.1432 2024 +2026 4 410.7326 596.4839 27.7799 0.1483 2025 +2027 4 411.7965 596.0698 27.608 0.1581 2026 +2028 4 412.8742 595.6831 27.4604 0.1738 2027 +2029 4 413.9438 595.2804 27.326 0.2151 2028 +2030 4 414.9391 594.7404 27.1802 0.2321 2029 +2031 4 415.7262 593.9362 26.9903 0.2099 2030 +2032 4 416.3885 593.0164 26.7498 0.2009 2031 +2033 4 417.1402 592.1824 26.465 0.2569 2032 +2034 4 418.0599 591.5452 26.1405 0.236 2033 +2035 4 419.093 591.0991 25.8079 0.2317 2034 +2036 4 420.2049 590.8829 25.5203 0.2855 2035 +2037 4 421.3455 590.7936 25.2949 0.3135 2036 +2038 4 422.1657 590.2148 25.1138 0.2564 2037 +2039 4 422.8167 589.2824 24.9466 0.2217 2038 +2040 4 423.7891 588.8134 24.7453 0.2695 2039 +2041 4 424.7764 588.2951 24.5171 0.2716 2040 +2042 4 425.8243 587.9291 24.2455 0.2382 2041 +2043 4 426.9488 587.8638 23.9072 0.2424 2042 +2044 4 428.0642 587.746 23.5136 0.2722 2043 +2045 4 429.1338 587.4417 23.0759 0.221 2044 +2046 4 430.0616 586.8789 22.5921 0.185 2045 +2047 4 431.0798 586.7576 22.0962 0.1758 2046 +2048 4 431.6678 587.6728 21.7202 0.2226 2047 +2049 4 432.6448 587.0779 21.4444 0.228 2048 +2050 4 433.6549 586.546 21.245 0.2897 2049 +2051 4 434.736 586.2863 21.0893 0.268 2050 +2052 4 435.8411 586.5574 20.925 0.3153 2051 +2053 4 436.9325 586.8731 20.739 0.3228 2052 +2054 4 438.0021 587.2335 20.5246 0.2676 2053 +2055 4 439.1324 587.2495 20.3143 0.2726 2054 +2056 4 440.2478 587.1168 20.1158 0.2176 2055 +2057 4 441.3632 587.3319 19.9072 0.1995 2056 +2058 4 442.4717 587.6099 19.7254 0.2155 2057 +2059 4 443.586 587.8387 19.5742 0.2337 2058 +2060 4 444.563 587.5801 19.434 0.2091 2059 +2061 4 444.9485 586.5288 19.2634 0.2172 2060 +2062 4 445.4553 585.6548 19.0585 0.201 2061 +2063 4 446.5181 585.2532 18.8479 0.2078 2062 +2064 4 447.5236 584.7659 18.6007 0.2703 2063 +2065 4 448.1814 583.9205 18.277 0.2583 2064 +2066 4 449.1573 584.0772 17.964 0.285 2065 +2067 4 450.2681 584.2545 17.6336 0.326 2066 +2068 4 451.2199 583.6745 17.3216 0.3288 2067 +2069 4 452.2312 583.1677 17.0075 0.3461 2068 +2070 4 453.358 583.3039 16.7418 0.3192 2069 +2071 4 454.4929 583.3016 16.4909 0.3263 2070 +2072 4 455.5397 582.8714 16.2484 0.2924 2071 +2073 4 456.4766 582.2296 15.755 0.2288 2072 +2074 4 390.8796 600.0269 31.1041 0.1797 2007 +2075 4 391.2308 598.9538 31.2757 0.1502 2074 +2076 4 391.5809 597.8807 31.512 0.1376 2075 +2077 4 392.0477 596.8385 31.7447 0.138 2076 +2078 4 392.5945 595.8341 31.9284 0.1384 2077 +2079 4 393.1436 594.8308 32.0589 0.1395 2078 +2080 4 393.4605 593.7326 32.1479 0.1414 2079 +2081 4 393.6458 592.6034 32.2151 0.1449 2080 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2102 +2104 4 373.5595 580.2871 35.2439 0.1372 2103 +2105 4 372.5539 579.7426 35.343 0.1372 2104 +2106 4 371.6787 579.0093 35.4242 0.1371 2105 +2107 4 370.8379 578.2325 35.4903 0.1368 2106 +2108 4 369.9993 577.4546 35.5485 0.1364 2107 +2109 4 369.1608 576.6767 35.6135 0.1356 2108 +2110 4 368.2227 576.0234 35.686 0.1342 2109 +2111 4 367.2194 575.472 35.7658 0.1313 2110 +2112 4 366.1143 575.2021 35.8873 0.1271 2111 +2113 4 364.984 575.1906 36.0651 0.1144 2112 +2114 4 363.8549 575.1803 36.5742 0.1144 2113 +2115 4 394.3391 589.3419 32.4814 0.1846 2083 +2116 4 394.6892 588.2631 32.6626 0.2325 2115 +2117 4 395.0381 587.1832 32.8756 0.2779 2116 +2118 4 395.4705 586.125 33.0649 0.229 2117 +2119 4 395.9224 585.0736 33.2192 0.2121 2118 +2120 4 396.3811 584.0269 33.3606 0.1669 2119 +2121 4 396.8433 582.9847 33.4986 0.15 2120 +2122 4 397.3078 581.9425 33.6344 0.1373 2121 +2123 4 397.8615 580.9415 33.7492 0.1373 2122 +2124 4 398.4152 579.9416 33.8512 0.1374 2123 +2125 4 398.9689 578.9406 33.9413 0.1374 2124 +2126 4 399.5226 577.9396 34.018 0.1376 2125 +2127 4 400.0774 576.9398 34.0838 0.1379 2126 +2128 4 400.6311 575.9388 34.1421 0.1383 2127 +2129 4 401.1848 574.9389 34.1964 0.1392 2128 +2130 4 401.7385 573.9379 34.2457 0.1409 2129 +2131 4 401.9444 572.6978 34.3011 0.144 2130 +2132 4 402.1961 571.5836 34.3176 0.1503 2131 +2133 4 402.41 570.4625 34.323 0.1589 2132 +2134 4 402.5221 569.3276 34.3101 0.1901 2133 +2135 4 402.6182 568.1905 34.2908 0.1732 2134 +2136 4 403.0037 567.162 34.3512 0.1621 2135 +2137 4 403.5506 566.1805 34.496 0.1571 2136 +2138 4 404.0711 565.168 34.6475 0.1867 2137 +2139 4 404.4566 564.095 34.7774 0.1669 2138 +2140 4 404.698 562.9784 34.886 0.15 2139 +2141 4 404.5664 561.8687 34.9706 0.1373 2140 +2142 4 404.4967 560.743 35.0431 0.1373 2141 +2143 4 404.6317 559.6105 35.1218 0.1373 2142 +2144 4 404.8033 558.4814 35.2139 0.1373 2143 +2145 4 404.9417 557.3476 35.3021 0.1373 2144 +2146 4 405.0526 556.2094 35.3718 0.1373 2145 +2147 4 405.1579 555.0699 35.4217 0.1373 2146 +2148 4 405.2631 553.9305 35.4547 0.1373 2147 +2149 4 405.3707 552.7922 35.4757 0.1373 2148 +2150 4 405.4816 551.6528 35.4914 0.1373 2149 +2151 4 405.5915 550.5145 35.5085 0.1373 2150 +2152 4 405.7253 549.3785 35.5309 0.1373 2151 +2153 4 406.0331 548.2837 35.567 0.1373 2152 +2154 4 406.4232 547.2084 35.6177 0.1373 2153 +2155 4 406.8133 546.1342 35.6782 0.1373 2154 +2156 4 407.2045 545.0588 35.7442 0.1373 2155 +2157 4 407.5946 543.9846 35.8117 0.1373 2156 +2158 4 407.9847 542.9092 35.8764 0.1373 2157 +2159 4 408.3874 541.8384 35.9293 0.1374 2158 +2160 4 408.8073 540.7745 35.9654 0.1375 2159 +2161 4 409.5772 539.9886 35.9876 0.1376 2160 +2162 4 410.5187 539.3388 35.9988 0.1379 2161 +2163 4 411.1044 538.4099 36.0024 0.1384 2162 +2164 4 411.4865 537.3322 36.0013 0.1393 2163 +2165 4 411.8572 536.25 35.9976 0.1411 2164 +2166 4 412.3525 535.2227 35.9923 0.1441 2165 +2167 4 412.8845 534.2102 35.9845 0.1506 2166 +2168 4 413.0218 533.1051 35.9736 0.1594 2167 +2169 4 412.9874 531.9611 35.959 0.1909 2168 +2170 4 412.9691 530.8171 35.9408 0.1748 2169 +2171 4 413.4874 529.8676 35.9078 0.1645 2170 +2172 4 414.2321 529.0016 35.8602 0.1646 2171 +2173 4 414.978 528.1345 35.8072 0.1859 2172 +2174 4 415.7227 527.2673 35.7585 0.2374 2173 +2175 4 416.4789 526.4105 35.7314 0.2744 2174 +2176 4 417.2465 525.5628 35.7389 0.2829 2175 +2177 4 418.0176 524.7173 35.7832 0.363 2176 +2178 4 418.6651 523.7861 35.894 0.4279 2177 +2179 4 419.1982 522.7897 36.0847 0.4967 2178 +2180 4 419.7553 521.8036 36.3278 0.5298 2179 +2181 4 420.4806 520.9296 36.5616 0.4785 2180 +2182 4 421.2643 520.0956 36.7688 0.3578 2181 +2183 4 421.667 519.082 37.0185 0.2585 2182 +2184 4 422.0319 518.0226 37.3024 0.2719 2183 +2185 4 422.7206 517.1338 37.5463 0.2523 2184 +2186 4 423.4756 516.2746 37.7476 0.2039 2185 +2187 4 424.0751 515.3102 37.9445 0.1713 2186 +2188 4 424.6253 514.3138 38.1391 0.1771 2187 +2189 4 425.1756 513.3174 38.3214 0.2094 2188 +2190 4 425.727 512.3198 38.4938 0.2793 2189 +2191 4 426.108 511.2479 38.6316 0.3596 2190 +2192 4 426.4443 510.1542 38.7282 0.405 2191 +2193 4 426.8722 509.0949 38.7881 0.4194 2192 +2194 4 427.3275 508.0458 38.8206 0.4418 2193 +2195 4 427.7828 506.9956 38.836 0.3902 2194 +2196 4 428.3365 505.9969 38.843 0.4034 2195 +2197 4 429.1533 505.2133 38.85 0.3574 2196 +2198 4 429.8786 504.3358 38.8604 0.2706 2197 +2199 4 430.0113 503.225 38.8746 0.2311 2198 +2200 4 430.0502 502.081 38.8945 0.2544 2199 +2201 4 430.6142 501.1132 38.9211 0.4013 2200 +2202 4 431.4116 500.2952 38.9575 0.3991 2201 +2203 4 431.7982 499.2256 39.0188 0.3553 2202 +2204 4 432.1609 498.1422 39.0995 0.2386 2203 +2205 4 432.5224 497.0588 39.1927 0.1946 2204 +2206 4 432.8953 495.9778 39.2907 0.191 2205 +2207 4 433.3278 494.9196 39.3739 0.2629 2206 +2208 4 433.7636 493.8614 39.4442 0.244 2207 +2209 4 434.1983 492.8032 39.5058 0.2611 2208 +2210 4 434.6434 491.7495 39.5685 0.2688 2209 +2211 4 435.1387 490.7211 39.6564 0.2844 2210 +2212 4 435.6375 489.6938 39.7681 0.3082 2211 +2213 4 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0.1401 2343 +2345 4 479.6918 347.9533 52.661 0.1425 2344 +2346 4 479.6918 346.8093 52.7604 0.1469 2345 +2347 4 479.6918 345.6653 52.8276 0.1556 2346 +2348 4 479.6918 344.5213 52.8752 0.1692 2347 +2349 4 479.6106 343.3842 52.906 0.2065 2348 +2350 4 479.4916 342.2493 52.9228 0.2164 2349 +2351 4 479.4939 341.1053 52.9351 0.1797 2350 +2352 4 479.6735 339.9865 52.953 0.1501 2351 +2353 4 480.003 338.8917 52.9774 0.1375 2352 +2354 4 480.3679 337.8106 53.0088 0.1377 2353 +2355 4 480.6001 336.7112 53.0474 0.1381 2354 +2356 4 480.8484 335.6324 53.1331 0.1388 2355 +2357 4 481.298 334.5925 53.2454 0.1399 2356 +2358 4 481.6309 333.5023 53.3355 0.1422 2357 +2359 4 481.7487 332.3709 53.4047 0.1463 2358 +2360 4 481.9192 331.2498 53.4565 0.1547 2359 +2361 4 482.3115 330.179 53.4943 0.1669 2360 +2362 4 482.76 329.1254 53.52 0.2053 2361 +2363 4 483.0517 328.026 53.543 0.1994 2362 +2364 4 483.1249 326.8888 53.5685 0.2202 2363 +2365 4 483.1249 325.7597 53.6469 0.2183 2364 +2366 4 483.1249 324.6237 53.7326 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531.9726 86.638 47.8276 0.3811 2719 +2721 4 532.9221 86.0128 47.8276 0.3057 2720 +2722 4 534.065 85.9612 47.8276 0.3385 2721 +2723 4 535.2078 85.9025 47.8276 0.3629 2722 +2724 4 536.3404 85.7452 47.8276 0.3562 2723 +2725 4 537.4695 85.575 47.8274 0.3671 2724 +2726 4 538.4522 85.0403 47.8274 0.3888 2725 +2727 4 539.0288 84.0522 47.8274 0.3067 2726 +2728 4 539.7232 83.1864 47.8271 0.2916 2727 +2729 4 540.8123 82.8359 47.8271 0.2842 2728 +2730 4 541.8853 82.441 47.8268 0.3989 2729 +2731 4 542.9378 82.0098 47.8262 0.4456 2730 +2732 4 544.0738 81.8907 47.826 0.4229 2731 +2733 4 545.1663 81.7585 47.8251 0.3391 2732 +2734 4 546.0118 80.9882 47.8243 0.3871 2733 +2735 4 546.9052 80.2817 47.8229 0.4031 2734 +2736 4 547.8982 79.7182 47.8206 0.33 2735 +2737 4 548.7219 78.9561 47.8181 0.236 2736 +2738 4 549.3282 78.0103 47.815 0.2027 2737 +2739 4 550.2869 77.4069 47.8097 0.2579 2738 +2740 4 551.3611 77.0122 47.7988 0.2497 2739 +2741 4 552.4124 76.5707 47.7862 0.1992 2740 +2742 4 553.3951 75.995 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533.2996 151.0343 57.4986 0.3488 2786 +2788 4 534.1004 150.3182 57.6414 0.348 2787 +2789 4 534.7182 149.4636 57.8385 0.3792 2788 +2790 4 535.3806 148.5656 58.0507 0.393 2789 +2791 4 536.3964 148.1354 58.2512 0.4035 2790 +2792 4 537.2945 147.481 58.4542 0.3808 2791 +2793 4 537.6892 146.4595 58.7661 0.43 2792 +2794 4 538.4774 145.7822 59.1982 0.422 2793 +2795 4 539.6214 145.7925 59.5647 0.4301 2794 +2796 4 540.5858 145.6301 59.9012 0.3342 2795 +2797 4 540.2529 144.6027 60.3042 0.352 2796 +2798 4 539.8547 143.7894 60.6567 0.3495 2797 +2799 4 540.8454 143.2894 60.9739 0.4035 2798 +2800 4 541.2847 142.2953 61.2424 0.4474 2799 +2801 4 541.2744 141.1513 61.4706 0.4586 2800 +2802 4 541.3969 140.0302 61.6739 0.3631 2801 +2803 4 542.0535 139.1859 61.8898 0.4074 2802 +2804 4 542.955 138.5064 62.134 0.3295 2803 +2805 4 543.8587 137.8863 62.4361 0.2752 2804 +2806 4 544.7019 137.2606 62.8365 0.1946 2805 +2807 4 544.3896 136.2584 63.1809 0.1777 2806 +2808 4 545.013 135.3787 63.446 0.1888 2807 +2809 4 545.8436 134.5939 63.6535 0.2315 2808 +2810 4 546.7256 133.8732 63.8352 0.318 2809 +2811 4 547.4578 133.0014 63.9881 0.443 2810 +2812 4 547.5321 131.8609 64.092 0.5038 2811 +2813 4 547.6019 130.7192 64.1777 0.5304 2812 +2814 4 547.6168 129.5752 64.2547 0.426 2813 +2815 4 547.5653 128.4323 64.3294 0.4065 2814 +2816 4 547.5012 127.2906 64.4104 0.3265 2815 +2817 4 547.3846 126.1523 64.5098 0.2764 2816 +2818 4 547.2988 125.0129 64.6554 0.2789 2817 +2819 4 547.2862 123.886 64.8886 0.3925 2818 +2820 4 547.2736 122.7604 65.1846 0.5318 2819 +2821 4 546.2737 122.4572 65.7947 0.4576 2820 +2822 4 545.2052 122.1197 66.3611 0.4578 2821 +2823 4 544.2191 121.5489 66.5731 0.4436 2822 +2824 4 543.5716 120.644 66.8175 0.3869 2823 +2825 4 543.3439 119.5572 67.1076 0.3154 2824 +2826 4 542.8383 118.6397 67.4114 0.2474 2825 +2827 4 543.1575 117.641 67.8266 0.2661 2826 +2828 4 542.844 116.6628 68.1946 0.285 2827 +2829 4 541.8648 116.124 68.5672 0.3965 2828 +2830 4 540.7802 115.8106 68.9646 0.4599 2829 +2831 4 539.7358 115.3884 69.3554 0.3584 2830 +2832 4 538.8217 114.7604 69.7654 0.3178 2831 +2833 4 537.9077 114.5247 70.2369 0.3278 2832 +2834 4 536.8369 114.5762 70.7176 0.3901 2833 +2835 4 536.0006 113.8684 71.1668 0.5209 2834 +2836 4 535.3039 112.9713 71.5918 0.5759 2835 +2837 4 534.7285 112.0097 72.0269 0.5579 2836 +2838 4 533.9345 111.2338 72.455 0.42 2837 +2839 4 533.2836 110.3156 72.8294 0.3291 2838 +2840 4 532.9564 109.267 73.2542 0.3904 2839 +2841 4 532.1224 108.5409 73.687 0.418 2840 +2842 4 531.0711 108.1176 74.0695 0.4292 2841 +2843 4 530.5792 107.3455 74.464 0.415 2842 +2844 4 531.1512 106.4428 74.965 0.445 2843 +2845 4 531.8799 105.6001 75.4975 0.3409 2844 +2846 4 532.1911 104.6193 76.0547 0.2365 2845 +2847 4 532.6464 103.7833 76.6514 0.1848 2846 +2848 4 533.7263 103.567 77.2075 0.2009 2847 +2849 4 534.4379 102.7834 77.6664 0.2599 2848 +2850 4 535.487 102.664 78.0707 0.3415 2849 +2851 4 536.6115 102.4967 78.428 0.2853 2850 +2852 4 537.2968 101.8433 78.8026 0.2725 2851 +2853 4 536.7648 101.0382 79.2803 0.2628 2852 +2854 4 537.084 100.0499 79.669 0.2918 2853 +2855 4 538.2097 99.915 79.9929 0.2313 2854 +2856 4 539.3148 100.1176 80.2922 0.2163 2855 +2857 4 540.3478 100.5582 80.6134 0.1747 2856 +2858 4 541.4243 100.8896 80.9203 0.1642 2857 +2859 4 542.3921 100.6952 81.1796 0.1642 2858 +2860 4 542.4997 99.7021 81.4299 0.1842 2859 +2861 4 542.4539 98.6687 81.6766 0.238 2860 +2862 4 543.2238 98.52 82.0302 0.2575 2861 +2863 4 544.1196 99.0111 82.5306 0.3397 2862 +2864 4 544.989 99.6767 83.0505 0.3836 2863 +2865 4 545.8424 100.4334 83.4893 0.4186 2864 +2866 4 546.7748 100.1769 83.9009 0.3667 2865 +2867 4 547.6843 100.7756 84.2072 0.2644 2866 +2868 4 548.5903 101.4746 84.4105 0.2192 2867 +2869 4 549.4952 102.1741 84.5426 0.2345 2868 +2870 4 550.4894 102.7309 84.6754 0.3543 2869 +2871 4 551.4995 103.2565 84.8271 0.3618 2870 +2872 4 552.6218 103.1462 84.9629 0.3867 2871 +2873 4 553.7486 103.2533 85.0993 0.3805 2872 +2874 4 554.673 103.9222 85.251 0.394 2873 +2875 4 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481.2568 123.0544 62.3246 0.1716 3314 +3316 4 481.076 121.9355 62.2745 0.1716 3315 +3317 4 481.0646 120.8075 62.1592 0.1716 3316 +3318 4 481.1927 119.6727 62.0603 0.1716 3317 +3319 4 481.2877 118.5321 61.9833 0.1716 3318 +3320 4 481.2934 117.3893 61.927 0.1716 3319 +3321 4 481.2934 116.2453 61.8887 0.1716 3320 +3322 4 481.4936 115.1196 61.8649 0.1716 3321 +3323 4 481.7373 114.0019 61.8506 0.1716 3322 +3324 4 481.7304 112.8593 61.8332 0.1716 3323 +3325 4 481.0943 111.9184 61.8139 0.1716 3324 +3326 4 480.4079 111.006 61.7907 0.1716 3325 +3327 4 479.574 110.2562 61.7016 0.1716 3326 +3328 4 478.5832 109.7009 61.6328 0.1716 3327 +3329 4 477.8339 108.8421 61.6 0.1716 3328 +3330 4 476.9542 108.156 61.6557 0.1716 3329 +3331 4 475.944 107.6371 61.7095 0.1716 3330 +3332 4 475.0895 106.8781 61.7546 0.1716 3331 +3333 4 474.3207 106.0334 61.7873 0.1716 3332 +3334 4 473.6629 105.0991 61.7957 0.1716 3333 +3335 4 473.0554 104.1297 61.78 0.1716 3334 +3336 4 472.5189 103.13 61.7389 0.1716 3335 +3337 4 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60.5545 0.1716 3358 +3360 4 461.787 80.336 60.4708 0.1716 3359 +3361 4 461.048 79.5467 60.3487 0.1716 3360 +3362 4 459.9555 79.2197 60.1745 0.1716 3361 +3363 4 458.863 78.9097 59.9463 0.1716 3362 +3364 4 457.7716 78.6522 59.642 0.1716 3363 +3365 4 456.8999 78.1697 59.2967 0.1716 3364 +3366 4 456.3954 77.1919 58.9649 0.1716 3365 +3367 4 455.4619 76.6028 58.6256 0.1716 3366 +3368 4 454.4563 76.1003 58.3167 0.1716 3367 +3369 4 453.5056 75.4807 58.0303 0.1716 3368 +3370 4 452.5035 75.4769 57.7808 0.1716 3369 +3371 4 451.4968 75.4404 57.5252 0.1716 3370 +3372 4 450.6822 74.697 57.2709 0.1716 3371 +3373 4 449.7625 74.2661 56.9828 0.1716 3372 +3374 4 448.6471 74.2639 56.6415 0.1716 3373 +3375 4 447.6861 73.7005 56.3399 0.1716 3374 +3376 4 447.86 72.5984 56.0784 0.1716 3375 +3377 4 448.1917 71.504 55.8438 0.1716 3376 +3378 4 447.312 70.9581 55.5453 0.1716 3377 +3379 4 446.4414 70.5473 55.1939 0.1716 3378 +3380 4 447.0043 69.7237 54.8484 0.1716 3379 +3381 4 447.6449 68.7976 54.4681 0.1716 3380 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123.0921 64.4137 0.183 3448 +3450 4 478.9825 122.1655 64.8096 0.183 3449 +3451 4 478.1188 121.431 65.109 0.183 3450 +3452 4 477.008 121.2491 65.3192 0.183 3451 +3453 4 476.2689 120.4449 65.5138 0.183 3452 +3454 4 475.7644 119.4222 65.6779 0.183 3453 +3455 4 475.1924 118.4315 65.7961 0.183 3454 +3456 4 474.442 117.5689 65.8938 0.183 3455 +3457 4 473.9432 116.5404 65.984 0.183 3456 +3458 4 473.2739 115.6195 66.0694 0.183 3457 +3459 4 472.3061 115.0303 66.1606 0.183 3458 +3460 4 471.6472 114.1268 66.3368 0.183 3459 +3461 4 470.9093 113.28 66.5588 0.183 3460 +3462 4 470.2286 112.3616 66.7372 0.183 3461 +3463 4 469.5823 111.4307 66.8763 0.183 3462 +3464 4 468.6865 110.7207 66.981 0.183 3463 +3465 4 467.7324 110.0903 67.0552 0.183 3464 +3466 4 466.776 109.4643 67.1062 0.183 3465 +3467 4 465.8174 108.8693 67.1754 0.183 3466 +3468 4 464.8175 108.3784 67.291 0.183 3467 +3469 4 463.9069 107.6892 67.3837 0.183 3468 +3470 4 463.0397 106.9704 67.4548 0.183 3469 +3471 4 462.3064 106.0974 67.5069 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135.3672 63.9996 0.3183 3717 +3719 4 520.3484 135.2494 64.1567 0.2926 3718 +3720 4 521.473 135.1762 64.3006 0.2707 3719 +3721 4 522.379 134.5687 64.3644 0.3339 3720 +3722 4 522.5117 133.7187 64.6582 0.2907 3721 +3723 4 523.3617 133.1868 64.9947 0.3026 3722 +3724 4 524.4062 132.7337 65.3033 0.3355 3723 +3725 4 525.3054 132.0393 65.5634 0.344 3724 +3726 4 526.1359 131.2546 65.7664 0.3861 3725 +3727 4 526.9596 130.4606 65.9151 0.3362 3726 +3728 4 527.7844 129.6667 66.003 0.2926 3727 +3729 4 528.8334 129.28 66.1097 0.318 3728 +3730 4 529.8367 128.7526 66.2435 0.4212 3729 +3731 4 530.0804 127.6578 66.3645 0.4542 3730 +3732 4 530.3378 126.5424 66.4756 0.4841 3731 +3733 4 530.991 125.6089 66.5795 0.4613 3732 +3734 4 531.6637 124.6823 66.6767 0.4562 3733 +3735 4 532.5514 123.9844 66.8242 0.495 3734 +3736 4 533.5719 123.5623 67.058 0.4475 3735 +3737 4 534.1862 122.5544 67.3907 0.3665 3736 +3738 4 534.7925 121.5843 67.4988 0.4113 3737 +3739 4 535.4435 120.644 67.5744 0.4635 3738 +3740 4 536.0395 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66.663 0.1781 3761 +3763 4 542.7067 97.0471 66.4149 0.1705 3762 +3764 4 542.6507 95.9299 66.1083 0.1769 3763 +3765 4 542.6987 94.8258 65.7258 0.2027 3764 +3766 4 542.6255 93.7176 65.2994 0.2969 3765 +3767 4 542.0512 92.8706 64.8824 0.2477 3766 +3768 4 541.0033 92.4248 64.4952 0.2127 3767 +3769 4 540.0492 91.8591 64.0934 0.2186 3768 +3770 4 539.6957 90.8963 63.7022 0.2281 3769 +3771 4 539.7998 89.7611 63.3587 0.254 3770 +3772 4 538.8984 89.1637 62.9894 0.3758 3771 +3773 4 538.1033 88.404 62.5932 0.357 3772 +3774 4 538.1662 87.3094 62.2098 0.2517 3773 +3775 4 537.3139 86.7423 61.8892 0.1706 3774 +3776 4 536.822 85.722 61.6025 0.1517 3775 +3777 4 536.1333 84.8184 61.3026 0.1652 3776 +3778 4 535.3085 84.0438 61.0033 0.1836 3777 +3779 4 534.8966 83.2147 60.7082 0.2492 3778 +3780 4 534.7079 82.2966 60.3562 0.2182 3779 +3781 4 533.6977 81.9301 59.8797 0.2154 3780 +3782 4 532.9827 82.4003 59.2256 0.1731 3781 +3783 4 533.1612 83.3279 58.5038 0.1612 3782 +3784 4 534.0329 83.5079 57.7867 0.1586 3783 +3785 4 534.7983 82.6607 57.1648 0.1746 3784 +3786 4 535.4972 81.7796 56.6272 0.2166 3785 +3787 4 535.4675 80.7654 56.1095 0.2349 3786 +3788 4 534.7697 79.9965 55.552 0.2152 3787 +3789 4 534.0741 79.2794 54.9637 0.2103 3788 +3790 4 533.6691 78.2196 54.4888 0.2758 3789 +3791 4 533.1326 77.3139 54.1108 0.2646 3790 +3792 4 532.0595 77.0501 53.79 0.3156 3791 +3793 4 531.3697 76.3839 53.5514 0.2901 3792 +3794 4 531.3846 75.2822 53.3924 0.3691 3793 +3795 4 532.1442 74.5855 53.2314 0.3599 3794 +3796 4 531.8994 73.9157 53.0813 0.2974 3795 +3797 4 530.9579 73.274 52.9673 0.2875 3796 +3798 4 529.8985 72.8577 52.883 0.3268 3797 +3799 4 528.8083 72.5116 52.817 0.3485 3798 +3800 4 527.7238 72.1492 52.7568 0.2948 3799 +3801 4 526.6553 71.7476 52.6865 0.3068 3800 +3802 4 525.5914 71.3326 52.5986 0.3605 3801 +3803 4 524.516 70.9492 52.5081 0.4213 3802 +3804 4 523.4235 70.6116 52.435 0.3783 3803 +3805 4 522.3264 70.2871 52.383 0.3689 3804 +3806 4 521.1961 70.1744 52.3508 0.3534 3805 +3807 4 520.0555 70.2435 52.3345 0.3156 3806 +3808 4 518.9161 70.3487 52.3289 0.2886 3807 +3809 4 517.7733 70.3571 52.3278 0.259 3808 +3810 4 516.6441 70.1974 52.3272 0.3334 3809 +3811 4 515.5184 69.9912 52.3264 0.3014 3810 +3812 4 514.3893 69.8091 52.3253 0.2655 3811 +3813 4 513.2567 69.6441 52.3236 0.2 3812 +3814 4 512.1242 69.4821 52.3214 0.1884 3813 +3815 4 510.9996 69.2779 52.3183 0.2044 3814 +3816 4 509.8934 68.9878 52.3141 0.282 3815 +3817 4 508.8112 68.6194 52.3079 0.307 3816 +3818 4 507.7587 68.1712 52.2995 0.244 3817 +3819 4 506.887 67.452 52.2878 0.2015 3818 +3820 4 506.0415 66.682 52.2707 0.219 3819 +3821 4 504.9982 66.2488 52.2472 0.2406 3820 +3822 4 503.9114 65.8898 52.2175 0.2193 3821 +3823 4 502.812 65.5755 52.1763 0.249 3822 +3824 4 501.6806 65.4856 52.0979 0.197 3823 +3825 4 500.5503 65.3515 51.9949 0.1634 3824 +3826 4 499.5631 64.8035 51.9084 0.1387 3825 +3827 4 498.5907 64.2001 51.8445 0.1397 3826 +3828 4 497.5954 63.6376 51.8011 0.1419 3827 +3829 4 496.5658 63.1396 51.7765 0.1456 3828 +3830 4 495.4515 62.9024 51.7675 0.1533 3829 +3831 4 494.3247 62.7082 51.7664 0.1645 3830 +3832 4 493.3065 62.2035 51.7664 0.2003 3831 +3833 4 492.2735 61.7119 51.7664 0.1931 3832 +3834 4 491.1718 61.4143 51.7664 0.1933 3833 +3835 4 490.061 61.1395 51.7664 0.2431 3834 +3836 4 489.3574 60.2742 51.7664 0.2102 3835 +3837 4 488.7385 59.3122 51.7664 0.1851 3836 +3838 4 488.1231 58.3472 51.7664 0.1917 3837 +3839 4 487.1907 57.702 51.7664 0.1761 3838 +3840 4 486.1462 57.2356 51.7664 0.1674 3839 +3841 4 485.0983 56.7783 51.7664 0.1675 3840 +3842 4 483.9967 56.4717 51.7664 0.2034 3841 +3843 4 482.8835 56.2085 51.7664 0.2105 3842 +3844 4 482.4271 55.1594 51.7664 0.1694 3843 +3845 4 481.9718 54.1101 51.7664 0.1271 3844 +3846 4 481.5153 53.061 51.7664 0.1144 3845 +3847 4 542.5866 99.4322 68.64 0.3432 3759 +3848 4 542.2205 98.3619 68.8534 0.2963 3847 +3849 4 541.9414 97.2558 69.078 0.3361 3848 +3850 4 541.7504 96.1279 69.2826 0.2855 3849 +3851 4 541.5593 94.9999 69.475 0.2239 3850 +3852 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81.8071 80.7184 0.1373 3873 +3875 4 528.4445 82.373 81.0984 0.1373 3874 +3876 4 527.5213 82.491 81.5738 0.1373 3875 +3877 4 526.844 81.7944 82.0996 0.1373 3876 +3878 4 526.8932 82.9131 82.6468 0.1373 3877 +3879 4 526.6862 83.9612 83.2098 0.1373 3878 +3880 4 525.6657 84.4335 83.7603 0.1373 3879 +3881 4 524.6819 84.7588 84.2864 0.1373 3880 +3882 4 524.0275 83.8513 84.7809 0.1373 3881 +3883 4 523.2679 83.1877 85.2404 0.1373 3882 +3884 4 522.2131 83.6217 85.6092 0.1373 3883 +3885 4 521.0977 83.7764 85.9468 0.1373 3884 +3886 4 519.988 83.5853 86.301 0.1373 3885 +3887 4 518.9905 83.1828 86.6536 0.1373 3886 +3888 4 518.2457 82.4505 86.9565 0.1373 3887 +3889 4 517.2459 82.8623 87.2998 0.1373 3888 +3890 4 516.246 82.863 87.761 0.1373 3889 +3891 4 515.4166 82.1329 88.1709 0.1373 3890 +3892 4 514.4076 81.6246 88.5408 0.1373 3891 +3893 4 513.3608 81.1731 88.8854 0.1373 3892 +3894 4 512.5257 80.4273 89.2382 0.1373 3893 +3895 4 512.0796 79.4129 89.614 0.1373 3894 +3896 4 511.6609 78.3823 90.0021 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802.5732 35.4673 0.1373 4795 +4797 3 405.3581 803.4747 35.4746 0.1373 4796 +4798 3 406.096 804.3487 35.485 0.1373 4797 +4799 3 407.0226 805.0031 35.4995 0.1373 4798 +4800 3 408.0053 805.5876 35.5194 0.1373 4799 +4801 3 408.988 806.1722 35.5454 0.1373 4800 +4802 3 409.7705 806.9879 35.588 0.1373 4801 +4803 3 410.4718 807.8917 35.6485 0.1373 4802 +4804 3 411.1673 808.7988 35.7224 0.1373 4803 +4805 3 411.8629 809.706 35.8056 0.1373 4804 +4806 3 412.5573 810.6144 35.894 0.1373 4805 +4807 3 413.3226 811.4621 35.9836 0.1373 4806 +4808 3 414.2538 812.1142 36.0696 0.1373 4807 +4809 3 415.2262 812.7148 36.1508 0.1373 4808 +4810 3 416.1998 813.3142 36.2261 0.1373 4809 +4811 3 417.1745 813.9137 36.295 0.1373 4810 +4812 3 418.1469 814.5143 36.3563 0.1373 4811 +4813 3 419.1204 815.1137 36.4095 0.1374 4812 +4814 3 420.1203 815.6697 36.4479 0.1374 4813 +4815 3 421.1075 816.2463 36.463 0.1376 4814 +4816 3 422.0342 816.9144 36.4526 0.1379 4815 +4817 3 422.9448 817.6054 36.4246 0.1383 4816 +4818 3 423.8543 818.2975 36.3899 0.1392 4817 +4819 3 424.7649 818.9885 36.3583 0.1409 4818 +4820 3 425.6595 819.7012 36.3418 0.144 4819 +4821 3 426.3688 820.5855 36.3796 0.1499 4820 +4822 3 427.0289 821.5144 36.4697 0.1604 4821 +4823 3 427.6878 822.4445 36.596 0.1811 4822 +4824 3 428.3468 823.3746 36.7424 0.2142 4823 +4825 3 429.0103 824.3046 36.8861 0.3012 4824 +4826 3 429.6795 825.2313 36.9972 0.3376 4825 +4827 3 430.3511 826.1568 37.0703 0.3256 4826 +4828 3 431.0226 827.0834 37.1134 0.2307 4827 +4829 3 431.8692 827.843 37.1353 0.1787 4828 +4830 3 432.8244 828.4688 37.1445 0.1678 4829 +4831 3 433.7956 829.074 37.149 0.1893 4830 +4832 3 434.7246 829.7398 37.1538 0.2566 4831 +4833 3 435.5814 830.4971 37.1602 0.2473 4832 +4834 3 436.6842 830.719 37.1697 0.1948 4833 +4835 3 437.7825 831.0348 37.1826 0.1546 4834 +4836 3 438.454 831.942 37.2008 0.1455 4835 +4837 3 438.7 833.0574 37.226 0.1532 4836 +4838 3 438.9196 834.1796 37.2613 0.1642 4837 +4839 3 438.9208 835.3225 37.3122 0.1997 4838 +4840 3 438.9093 836.4665 37.3831 0.1922 4839 +4841 3 438.8613 837.6105 37.4777 0.1915 4840 +4842 3 438.8121 838.7533 37.5967 0.2398 4841 +4843 3 438.6988 839.8699 37.814 0.2033 4842 +4844 3 438.573 840.9761 38.1147 0.1749 4843 +4845 3 438.446 842.0824 38.4667 0.161 4844 +4846 3 438.4632 843.2127 38.8091 0.1759 4845 +4847 3 438.7606 844.3166 39.0628 0.2344 4846 +4848 3 439.0592 845.4217 39.2344 0.1935 4847 +4849 3 439.455 846.4948 39.3316 0.1567 4848 +4850 3 439.8669 847.5621 39.3756 0.1271 4849 +4851 3 440.2776 848.6295 39.3876 0.1144 4850 +4852 3 440.6894 849.6968 39.3876 0.1144 4851 diff --git a/examples/bio_all_active_sweep/network/bio_fullaxon_node_types.csv b/examples/bio_all_active_sweep/network/bio_fullaxon_node_types.csv new file mode 100644 index 00000000..22fb7b0f --- /dev/null +++ b/examples/bio_all_active_sweep/network/bio_fullaxon_node_types.csv @@ -0,0 +1,2 @@ +node_type_id model_processing model_type morphology specimen_id model_template dynamics_params +100 aibs_allactive_fullaxon biophysical reconstruction.swc 488683425 ctdb:Biophys1.hoc fit_parameters.json diff --git a/examples/bio_all_active_sweep/network/bio_fullaxon_nodes.h5 b/examples/bio_all_active_sweep/network/bio_fullaxon_nodes.h5 new file mode 100644 index 00000000..158e8204 Binary files /dev/null and b/examples/bio_all_active_sweep/network/bio_fullaxon_nodes.h5 differ diff --git a/examples/bio_all_active_sweep/network/bio_stubaxon_node_types.csv b/examples/bio_all_active_sweep/network/bio_stubaxon_node_types.csv new file mode 100644 index 00000000..335dd688 --- /dev/null +++ b/examples/bio_all_active_sweep/network/bio_stubaxon_node_types.csv @@ -0,0 +1,2 @@ +node_type_id model_processing model_type morphology specimen_id model_template dynamics_params +100 aibs_allactive biophysical reconstruction.swc 488683425 ctdb:Biophys1.hoc fit_parameters.json diff --git a/examples/bio_all_active_sweep/network/bio_stubaxon_nodes.h5 b/examples/bio_all_active_sweep/network/bio_stubaxon_nodes.h5 new file mode 100644 index 00000000..1479a081 Binary files /dev/null and b/examples/bio_all_active_sweep/network/bio_stubaxon_nodes.h5 differ diff --git a/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/membrane_potential.h5 b/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/membrane_potential.h5 new file mode 100644 index 00000000..fce1c994 Binary files /dev/null and b/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/membrane_potential.h5 differ diff --git a/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/spikes.csv b/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/spikes.csv new file mode 100644 index 00000000..041d3bc6 --- /dev/null +++ b/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/spikes.csv @@ -0,0 +1,4 @@ +timestamps population node_ids +1097.09999999965 bio_fullaxon 0 +1396.5999999993776 bio_fullaxon 0 +1719.1999999990842 bio_fullaxon 0 diff --git a/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/spikes.h5 b/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/spikes.h5 new file mode 100644 index 00000000..c2a64705 Binary files /dev/null and b/examples/bio_all_active_sweep/output_491766131_fullaxon_sweep35/spikes.h5 differ diff --git a/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/membrane_potential.h5 b/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/membrane_potential.h5 new file mode 100644 index 00000000..60642733 Binary files /dev/null and b/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/membrane_potential.h5 differ diff --git a/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/spikes.csv b/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/spikes.csv new file mode 100644 index 00000000..80914ddd --- /dev/null +++ b/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/spikes.csv @@ -0,0 +1,5 @@ +timestamps population node_ids +1095.6999999996513 bio_stubaxon 0 +1297.099999999468 bio_stubaxon 0 +1549.4999999992383 bio_stubaxon 0 +1801.799999999009 bio_stubaxon 0 diff --git a/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/spikes.h5 b/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/spikes.h5 new file mode 100644 index 00000000..bd28f0f6 Binary files /dev/null and b/examples/bio_all_active_sweep/output_491766131_stubaxon_sweep35/spikes.h5 differ diff --git a/examples/bio_all_active_sweep/run_bionet.py b/examples/bio_all_active_sweep/run_bionet.py new file mode 100644 index 00000000..2b44e3f1 --- /dev/null +++ b/examples/bio_all_active_sweep/run_bionet.py @@ -0,0 +1,43 @@ +import sys + +from bmtk.simulator import bionet +from bmtk.simulator.bionet.io_tools import io +from bmtk.simulator.bionet.default_setters.cell_models import set_params_allactive +# from bmtk.simulator.bionet.pyfunction_cache import add_cell_processor +from bmtk.simulator.bionet import model_processing +from bmtk.analyzer.compartment import plot_traces + + +@model_processing +def aibs_allactive_fullaxon(hobj, cell, dynamics_params): + # This is essentially the same method used to intilize cell parameters as found + # in bmtk.simulator.bionet.default_setters.cell_models.aibs_allactive function. + # The main difference is that in the original the axon is cut and replaced by a + # stub. Here we leave the full axon intact + io.log_info('Initializing Cell Model Params') + # fix_axon_allactive(hobj) + set_params_allactive(hobj, dynamics_params) + return hobj + + +def run(config_path): + conf = bionet.Config.from_json(config_path, validate=True) + conf.build_env() + + graph = bionet.BioNetwork.from_config(conf) + sim = bionet.BioSimulator.from_config(conf, network=graph) + sim.run() + + plot_traces(config_file=config_path, report_name='membrane_potential') + # bionet.nrn.quit_execution() + + +if __name__ == '__main__': + if __file__ != sys.argv[-1]: + config_path = sys.argv[-1] + run(config_path) + else: + # run('config.simulation_syns.json') + # run('config.simulation.491766131_stubaxon.sweep35.json') + run('config.simulation.491766131_fullaxon.sweep35.json') +